CN106689066A - Method for quickly breeding aphidius gifuensis - Google Patents
Method for quickly breeding aphidius gifuensis Download PDFInfo
- Publication number
- CN106689066A CN106689066A CN201611156232.1A CN201611156232A CN106689066A CN 106689066 A CN106689066 A CN 106689066A CN 201611156232 A CN201611156232 A CN 201611156232A CN 106689066 A CN106689066 A CN 106689066A
- Authority
- CN
- China
- Prior art keywords
- aphid
- branch
- honeybee
- tree
- aphidius gifuensis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241001305034 Aphidius gifuensis Species 0.000 title claims abstract description 72
- 238000009395 breeding Methods 0.000 title claims abstract description 40
- 230000001488 breeding effect Effects 0.000 title claims abstract description 40
- 238000000034 method Methods 0.000 title claims abstract description 30
- 241001124076 Aphididae Species 0.000 claims abstract description 228
- 241000256844 Apis mellifera Species 0.000 claims abstract description 150
- 241000196324 Embryophyta Species 0.000 claims abstract description 101
- 235000006040 Prunus persica var persica Nutrition 0.000 claims abstract description 45
- 244000144730 Amygdalus persica Species 0.000 claims abstract description 40
- 230000024241 parasitism Effects 0.000 claims abstract description 21
- 235000002413 Prunus ansu Nutrition 0.000 claims abstract description 14
- 235000002637 Nicotiana tabacum Nutrition 0.000 claims abstract description 12
- 239000003337 fertilizer Substances 0.000 claims abstract description 3
- 244000061176 Nicotiana tabacum Species 0.000 claims abstract 3
- 244000018633 Prunus armeniaca Species 0.000 claims description 59
- 235000009827 Prunus armeniaca Nutrition 0.000 claims description 58
- 235000019504 cigarettes Nutrition 0.000 claims description 44
- 230000012010 growth Effects 0.000 claims description 31
- 230000003071 parasitic effect Effects 0.000 claims description 27
- 238000011081 inoculation Methods 0.000 claims description 21
- 238000005286 illumination Methods 0.000 claims description 16
- 230000032669 eclosion Effects 0.000 claims description 15
- 239000002689 soil Substances 0.000 claims description 15
- 239000007788 liquid Substances 0.000 claims description 13
- 210000003608 fece Anatomy 0.000 claims description 12
- 239000010871 livestock manure Substances 0.000 claims description 12
- 238000013138 pruning Methods 0.000 claims description 12
- 235000016709 nutrition Nutrition 0.000 claims description 11
- 230000035764 nutrition Effects 0.000 claims description 11
- 230000004083 survival effect Effects 0.000 claims description 10
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 claims description 8
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 claims description 7
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 claims description 7
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 claims description 7
- 241000819999 Nymphes Species 0.000 claims description 7
- 239000007864 aqueous solution Substances 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 7
- 239000007921 spray Substances 0.000 claims description 7
- 239000011347 resin Substances 0.000 claims description 6
- 229920005989 resin Polymers 0.000 claims description 6
- 240000005809 Prunus persica Species 0.000 claims description 5
- 241000596168 Robbauera albescens Species 0.000 claims description 5
- 241000607479 Yersinia pestis Species 0.000 claims description 5
- 238000005520 cutting process Methods 0.000 claims description 5
- 201000010099 disease Diseases 0.000 claims description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 5
- 230000008901 benefit Effects 0.000 claims description 4
- TWFZGCMQGLPBSX-UHFFFAOYSA-N carbendazim Chemical group C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 4
- 238000001784 detoxification Methods 0.000 claims description 4
- 239000000618 nitrogen fertilizer Substances 0.000 claims description 4
- 238000010899 nucleation Methods 0.000 claims description 4
- 238000004321 preservation Methods 0.000 claims description 4
- 230000008569 process Effects 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 238000003306 harvesting Methods 0.000 claims description 3
- 208000015181 infectious disease Diseases 0.000 claims description 3
- 238000005086 pumping Methods 0.000 claims description 3
- 230000003716 rejuvenation Effects 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 206010044565 Tremor Diseases 0.000 claims description 2
- 230000002015 leaf growth Effects 0.000 claims description 2
- 230000035699 permeability Effects 0.000 claims description 2
- 238000002054 transplantation Methods 0.000 claims description 2
- 230000008859 change Effects 0.000 claims 1
- 230000002040 relaxant effect Effects 0.000 claims 1
- 230000014599 transmission of virus Effects 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 7
- 241000238631 Hexapoda Species 0.000 abstract description 5
- 238000005265 energy consumption Methods 0.000 abstract description 2
- 230000001788 irregular Effects 0.000 abstract description 2
- 230000009467 reduction Effects 0.000 abstract description 2
- 241000256816 Braconidae Species 0.000 abstract 1
- 241000721621 Myzus persicae Species 0.000 abstract 1
- 241000208125 Nicotiana Species 0.000 description 9
- 241001600407 Aphis <genus> Species 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 238000010276 construction Methods 0.000 description 4
- 244000045947 parasite Species 0.000 description 4
- 239000003814 drug Substances 0.000 description 3
- 230000002265 prevention Effects 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 235000013311 vegetables Nutrition 0.000 description 3
- 208000035240 Disease Resistance Diseases 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 238000007605 air drying Methods 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 235000012907 honey Nutrition 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000003612 virological effect Effects 0.000 description 2
- 241000256837 Apidae Species 0.000 description 1
- 235000008744 Brassica perviridis Nutrition 0.000 description 1
- 241000712024 Brassica rapa var. perviridis Species 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241001468872 Fumagospora capnodioides Species 0.000 description 1
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- 241001464837 Viridiplantae Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000000266 injurious effect Effects 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 235000006286 nutrient intake Nutrition 0.000 description 1
- 230000017448 oviposition Effects 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 230000029264 phototaxis Effects 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 239000002985 plastic film Substances 0.000 description 1
- 229920006255 plastic film Polymers 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 230000027272 reproductive process Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000004513 sizing Methods 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 230000002277 temperature effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Botany (AREA)
- Cultivation Of Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a method for quickly breeding aphidius gifuensis. The method includes steps of host plant cultivation, insect species breeding, bee species breeding and aphidius gifuensis breeding, a wild ansu apricot tree or peach tree seedling is selected as a host plant, and a puerile treetop produced from the seedling after water-fertilizer culture is subjected bee breeding for about 35-45 days, which is a reduction of 30 days when compared with using tobacco for seedling breeding. The host plant can be grafted with aphid after leaf buds germinate and leaves unfold on March of early spring, so that time for first-time breeding of aphidius gifuensis in tobacco-growing areas of Northeast China is shortened, and the problems of frost influence, high breeding energy consumption, low activity of aphidius gifuensis, staggering of bee breeding peak and aphid appearing peak and irregular mummified aphidius gifuensis forming are solved. By the method, bee breeding cost is lowered, and using range of aphidius gifuensis is widened; the host plant of five years old can breed more than 50 thousand myzus persicae, braconid parasitism rate is up to 80%, mummified aphid forming rate of the host plant is up to higher than 75%, and each host plant can breed mummified aphidius gifuensis and can prevent and control different crops by more than 60 mu.
Description
Technical field
The present invention relates to the technical field of biological control of agriculture and forestry insect, and in particular to one kind is in biological control agriculture and forestry injurious insect
During quickly bred aphidius gifuensis using tall graminaceous plant wild apricot tree or peach seedling method.
Background technology
Aphis propagation virosis, forms sooty mould, has a strong impact on the quality of agricultural product, and people mainly pass through spraying
The method of medicine of learning to farm is prevented and treated so that the drug resistance and the resistance to the action of a drug of aphid are constantly strengthening, and the usage amount of chemical pesticide is year by year
Increase, environment is polluted, the quality safety to agriculture and forestry product threatens, influence people's is healthy.By wild
Ansu apricot tree large-scaled propugation aphid parasite, effectively controls the generation of aphid, has reached the target of green health, sustainable development.
In Liaoning, aphid is that largely occur in the season of drought, and life habit has very big difference, Liaoning with south
It is again the solidification core base unit of the Chinese-style cigarette such as cigarette in cigarette, Guizhou in Fujian, but the whole province's vega is generally all subject to cigarette aphid
Harm, due to long-term use chemical insecticide, make the cigarette aphid resistance to the action of a drug strengthen, tobacco leaf security reduction.To solve the above problems,
The anti-technology that eliminates aphis of aphid parasite is promoted, has been to prevent the biological prevention that eliminates aphis using natural enemy insect, this technology is in China
Main Tobacco-growing Regions In South has obtained extensive use, and obtains better effects, and it is numerous to be mainly that host plant is carried out with crops such as tobacco, vegetables
Grow.Because northern area has larger difference with southern each cigarette district in weather conditions, this technology is implemented also to exist and is permitted in the north
Many problems.Northeast cigarette district frost-free period is up to 200~240 days, when being eliminated aphis using aphidius gifuensis are anti-, when first time expanding numerous,
Because temperature is low, host plant culture needs the long period, causes the problem of avoiding the peak hour of numerous honeybee peak period and aphid occurrence in peak period；
In the particularly northern time in the early spring, how warm the earth is also is trembled with fear, and is difficult to grow by low temperature effect green plants, and aphidius gifuensis expand numerous for the first time
Time is long, survival rate is low, it is impossible to meet being actually needed for modern agriculture and forestry green production.
The content of the invention
Present invention aim to overcome that the deficiency that prior art is present, there is provided one kind is using tall graminaceous plant wild apricot tree
Or the method that peach seedling quickly breeds aphidius gifuensis.Can be solved well northeast early spring temperature is low, Ji Zhuzhi using the present invention
Thing culture needs the time long, and peak period and the insect for breeding honeybee largely occur that the phase avoids the peak hour and the honeybee kind survival ability introduced is poor, post
The weak problem of natural disposition.
The present invention has selected cold-resistant, drought-enduring, wild manageability, has told green Qiao for blooming (late March) at first in the Northeast
Graminaceous plant wild apricot tree or peach seedling carry out numerous honeybee, as worm after local vega, field of vegetables flutter aphid, the stiff aphid detoxification caught
Kind, aphid kind, worm kind, aphid kind than introduction improve survival ability, and strong adaptability is fast using tall graminaceous plant scale first
The method of speed breeding.
The technical scheme is that：A kind of method of quick breeding aphidius gifuensis, its feature exists
In：Cultivated including host plant, worm kind is bred, honeybee kind is bred and bred with aphidius gifuensis, the host plant of selection is that Qiao Benke plants
Thing wild apricot tree or peach seedling, after carrying out liquid manure culture, the young tender of its pumping newly slightly carries out numerous honeybee, specifically breeds including as follows
Method：
(1) host plant is cultivated：The tall graminaceous plant of the strong no disease and pests harm of selection, wild apricot of its age of tree in 5~6 years
Tree, 2~3 years raw peach seedlings are transplanted, and Second Year spring concentration is that the nitrogenous aqueous solution of 750~1000ppm is poured,
Blade is promoted to sprout, grow；Second Year after transplant survival is cultivated by dwarf treatment, pruning and liquid manure, and wild apricot tree exists
Need to carry out determining hat, pruning, pinching in growth course and draw branch；
Reach more than 5 DEG C in ground temperature, young leaves sprout after by the host plant after transplanting is covered on the numerous honeybee bred in room
In canopy, in 10~20 DEG C of temperature, humidity 40~60% is cultivated host plant, is posted within 7~15 days when cultivating under the sufficient environment of illumination
Main plant grow 10~40 centimetres it is new slightly when, the 3rd~5 leaf is inoculated with for aphid after growing point；
(2) worm kind is bred：The cigarette aphid in field is gathered, is purificated and rejuvenated using tobacco seedlings or Luo Bumiao or Chinese cabbage seedling, prevent viral biography
Broadcast；Can be placed on aphid after detoxification in growth cabinet and cultivate standby by winter；
The easily parasitic wild apricot tree of aphid or peach transplantation of seedlings are chosen to after breeding room, is planted indoor selection host is bred
The strain number of thing 10~15%, is cultivated by the epidemic disaster condition in step (1)；When host plant young sprout growth reach it is predetermined
During 10~40 centimetres of length, collection III ages, IV ages are inoculated into what is cultivated not by parasitic aptery larvae aphid as aphid worm kind
The vacuum side of blade of the 3rd~5 leaf position after wild apricot tree or the new slightly growing point of 10~40 centimetres of peach seedling, each is newly slightly inoculated with 6~9
Head aphid, to 40000~60000, aphid adult breeds standby during nymph on host plant for every plant of tree cigarette aphid breeding；
By aphid worm kind, the vacuum side of blade raising of the 3rd~5 leaf position is bred 7~12 after growing point, control aphid inoculation
10~25 DEG C of numerous honeybee room temperature afterwards, humidity 40~60%, make cigarette aphid on wild apricot tree each new slightly branch reach 400~
500, every plant of upper cigarette aphid of tree reaches 40000~60000, and peach seedling individual plant cigarette aphid reaches 8000~10000, breeds mid-term
Spray a carbendazol to carry out disinfection, obtain aphid parasitism branch；
(3) honeybee kind is bred：In the aphidius gifuensis that autumn last year collection field is asked, using tobacco seedlings or the parasitic aphid of Chinese cabbage seedling,
Aphidius gifuensis are put into according to 1: 100 honeybee aphid ratio, kind of a honeybee child care is carried out in growth cabinet；
The wild apricot tree of 50~60% numbers that step (2) is obtained or the aphid parasitism branch of peach seedling, outer cover 80
~90 mesh fly nets, choose the stiff aphid of aphidius gifuensis, reject hyperparasite honeybee, collection sprouted wings into honeybee or the stiff aphid that will sprout wings
Artificial infection is carried out, each new slightly inoculation reaches 400~500 when wild apricot tree, and every plant is set up to 40000~60000, peach
When sapling individual plant cigarette aphid reaches 8000~10000, it is put into net by 1: 25~30 honeybee, aphid ratio and is manually connect honeybee, treats cigarette
Aphid parasite carries out parasitism after sprouting wings to aphid；
10~25 DEG C of the temperature of room, humidity 30~60%, into honeybee are bred in the control after aphidius gifuensis inoculation of wild apricot tree
Temperature control is in 15~25 DEG C, humidity 60~80% during emergence；Peach seedling aphidius gifuensis inoculation after temperature of shed be 10~
20 DEG C, humidity 70~80% is 22~30 DEG C, humidity 50~60% into temperature during bee eclosion；Simultaneously to host plant liquid manure
Cultivation is suitably managed, is cultivated and is formed within 7~15 days a large amount of deadlock aphids, thus obtained honeybee kind parasitism branch；
(4) aphidius gifuensis Ashmaed scale is bred：By remaining 90~85% the wild apricot trees or peach seedling of number in step (2),
The mesh fly net of outer cover 80~90, training orientation is carried out by the epidemic disaster condition in step (1)；By the CMC model aphid of step (2)
Worm parasitism branch：The aphid parasitism branch cultivated in selecting step (2) is inoculated into the wild apricot tree or peach of remainder
Seedling treated on parasitic branch, is taken and is trembled connection and forming the vacuum side of blade of new slightly the 3rd~5 leaf position branch of parasitic aphid gently
Shake, it is upper that each branch has 6~9 aphids to fall, and in dither process, III ages, IV age aphids is fallen and connect on branch
Aphid；
After connecing aphid 10~12 days, wild apricot tree reaches each branch breeding cigarette aphid 400~500, every plant 40000~
60000, when peach seedling individual plant cigarette aphid reaches 8000~10000, the honeybee kind parasitism branch cultivated in selecting step (3),
Carry out connecing honeybee according to 1: 25~30 honeybee aphid ratio, when on each branch aphidius gifuensis parasitic rate reach 70~80% by put honeybee；
(5) honeybee is put：The branch that stiff aphid will be formed is cut apart from the centimeters of leaf tip 30~40, ansu apricot with stiff aphid or
The reservoir of cuttage branch is placed in column type and puts honeybee by peach branch cuttage in the nitrogenous fertilizer reservoir that weight is 5 ‰
In device, the mesh fly net of bee-escape outer cover 50~60 after the stiff aphid on branch sprouts wings, flies out to vega to find from fly net and posts
Main, aphid is blocked in bee-escape；Putting can be used as breeding aphid next time after the aphid remained on the branch after honeybee is withdrawn deposit
Plant aphid；
(6) collection and preservation of aphidius gifuensis：After apricot or the new aphid nymph for slightly going up of peach are changed into stiff aphid, host is planted
The leaf harvest that thing carries stiff aphid gets off, and removes aphid remaining on blade, obtains stiff aphid blade, and stiff aphid blade is placed on into interior
Natural air drying 0.5~3 day, then the stiff aphid on air-dried stiff aphid blade is separated, it is fitted into appearance honeybee bottle, and honeybee bottle will be held in 1
It is stored refrigerated in~5 DEG C of environment.
The growth temperature of described wild apricot tree is 10~20 DEG C, humidity 40~60%, in the sufficient environment of illumination
Cultivated, when cultivating 7~15 days, wild apricot tree young sprout implements aphid inoculation when being up to 30~40 centimetres.
It is new slightly for carrying out numerous honeybee that described wild apricot tree sprouts, by determining hat, pruning, drawing branch and pinching to be trained
Support, fixed hat：To wild apricot tree in the cutting of 1.5 meters or so of the height of tree, tree crown radius is controlled at 1.5 meters, tree-like for ease of connecing aphid, connect
Honeybee operation, increases the happy shape of cup-shaped of branch permeability；Prune：Carried out before rudiment after transplanting, by each of wild apricot tree
Individual branch is pruned, and branch bounces back to the spring of the previous year slightly, to promote Second Year leaf bud to sprout；Draw branch：Sprouted in spring then
After bud, in time by position should not, quantity excessively consume the close crowded young shoot or spray of nutrition and erase, the branch for leaving is obtained sufficient
Nutrition and illumination, to the upright branch of vigorous growth, to before rudiment after the resin flowing, pulled into and relaxed growing way
Angle；Pinching：Position to being sprouted in tree crown is suitable, illumination condition is straight compared with the branch of excessive growth of benefit, more young sprout, extension branch, back
The golden mean of the Confucian school branch of vertical growth carries out pinching, and the pinching time is before resin flows.
Described wild apricot tree controls 10~20 DEG C of numerous honeybee temperature of shed after aphidius gifuensis inoculation, and humidity 40~
55%, it is 15~25 DEG C, humidity 60~80% into temperature during bee eclosion；Peach seedling aphidius gifuensis inoculation after temperature of shed be
10~20 DEG C, humidity 75~80%, into 22~27 DEG C of temperature, humidity 50~60% during bee eclosion.
The described host plant selection age of tree in 5~6 years, the wild apricot tree of healthy and strong no disease and pests harm, last year 10 below the moon
Ten days~early November, the soil that span is equal to by tether panel soil and with tree crown is transplanted to interior is bred, in spring next year late March
Put tree disk in order, the tree well of 8~10 centimetres of lower digging is standby；Before late April tree rudiment, tree disk in pour concentration be 750~
The nitrogenous aqueous solution of 1000ppm, promotes the leaf growth sprouted；Second Year after transplant survival is raw by dwarf treatment, pruning
Need to carry out determining hat, pruning, pinching in growth process and draw branch.
Described host plant selects 2~3 years raw peach seedling cuttage growth and breedings in Nutrition Soil, and Nutrition Soil composition is to educate
Seedling matrix and vega soil 1: 1 mixture by weight, 10~15 centimetres of cuttage seeding row spacing；Second Year spring concentration after surviving
For the nitrogenous aqueous solution of 750~1000ppm is poured, blade is promoted to sprout, grow；10~20 DEG C of temperature of cultivation, humidity 40~
60%, host plant is cultivated in the sufficient environment of illumination, cultivate 10, when peach seedling grows 20~30 centimetres new standby after a while
With；Once pruned every 30 days, liquid manure is cultivated, it is ensured that whole Growing season has host plant standby.
The present invention takes full advantage of existing cold-resistant, drought resisting examination material, by determining the technical finesses such as hat, sizing, pruning, drawing branch
Afterwards, the method for being managed using host plant training orientation, the management of aphid worm kind and aphidius gifuensis honeybee kind, it is ensured that in spring host
Plant fast culture, can reach the requirement of quick numerous honeybee.The weather conditions that make use of field aphid, cocoon honeybee to occur, it is ensured that
Early breeding, early preventing and treating, it is low cost, easily operated, solve the problems, such as that aphidius gifuensis expand hard to tackle for the first time in northeast, realize this
Item biological prevention is from laboratory to the developing goal of comprehensive industrialization of crop field popularization and application.
The present invention carries out numerous honeybee from the wild apricot tree and peach seedling of Northeast Mountain Areas as host plant, and examination material is easy to be obtained
Arrive, and cigarette aphid and aphidius gifuensis can be met for the first time in the ecological environment of northern low temperature using the new slightly quick breeding of pumping
The need for expansion is numerous.Due also to such host plant March in early spring leaf bud sprout, blade unfold after by connect aphid, substantially reduce
Aphidius gifuensis breed the time in the first time of northeast cigarette district, solve frost influence and breed high energy consumption, and techniqueflow is cumbersome, cigarette
Aphid parasite poor activity, aphidius gifuensis deadlock aphid form irregular problem.The method also reduces numerous honeybee expense simultaneously, can be preferably
Lifting aphidius gifuensis in the widely used scope of agricultural leaf, make aphidius gifuensis northeast cigarette district promote find one it is practicable
Method, it is maximally effective to solve the problems, such as time, cost, practicality.This method reproductive process about 35~45 days, educates with tobacco
Seedling is bred to compare and is reduced about 30 days.Raw strain host plant can cultivate cigarette aphid more than 50,000 within 5 years, and cocoon honeybee parasitic rate reaches 80%
More than, stiff every plant of host plant of aphid formation rate reaches more than 75%, and every plant of host plant breeds aphidius gifuensis deadlock aphid, and preventing and treating is different
Crops can reach more than 60 mu.
Specific embodiment
The present invention is elaborated below by way of specific embodiment.
(the host plant of embodiment 1：Wild apricot tree)
(1) room construction is bred：The numerous honeybee canopy of indoor setting is bred, numerous honeybee canopy uses the connector structure of the zinc-plated tubing of steelframe,
It is easily installed, dismantles, numerous honeybee canopy is provided with outer cover fly net, thermal insulation plastic film and shade net three-layer network altogether, with being incubated, shelter from heat or light,
The function of rain-proof.Whole numerous honeybee canopy 25m long × 20m wide × 3.54m high, construction area is 480m2Two Conjoined solar greenhouses；Its
Neutral intercolumniation 3m, span 6m, spring base 0.75m, whole greenhouse are provided with 4 sets of film-coiling devices and are separately mounted to greenhouse both sides and ceiling,
Using semi-automatic sun shading net device control sunshade net in the road of sliding door 2, front and rear each 1 road, canopy, to strengthen the stability of greenhouse, greatly
Fixed using a horizon bar per 3m in canopy；80~90 mesh fly nets are used as the cut-off of numerous honeybee canopy in canopy, if being divided into
Between dry numerous honeybee.The aisle for staying 1.5m wide in the middle of greenhouse, both sides are divided into length × width × height respectively is：6m × 6m × 1.gm's is numerous
12 between honeybee, 4 between numerous honeybee of 6m × 3m × 1.gm, numerous honeybee is interior to transplant 3~4 years raw 56 plants of ansu apricot trees respectively, and every with 80
~90 mesh fly nets are spaced, and every plant is bred cigarette aphid more than 40000~60000, and breeding aphidius gifuensis are 35000~50000.
(2) host plant cultivating and managing：Requirement of the present invention to host plant kind be：Growth cycle is long, without frequent
Plantation, transplants in spring numerous honeybee, and sprouting after early, host plant applies rich water newly slightly can constantly increase, aphid eating, winter resistance
By force, disease resistance is strong, and blade is more, blade face is smooth, beneficial to the breeding of cigarette aphid.By screening host plant from the tall wild mountain of graminaceous plant
Apricot or peach seedling.
Wild apricot tree, strong with cold-resistant, drought resisting, disease resistance, aphid is easily parasitic, and blade is more, blade face is smooth.Selection physical features
It is flat, the age of tree in 5~6 years, the tall graminaceous plant wild apricot tree of healthy and strong no disease and pests harm, on late October last year~November
Ten days, high 1~1.5 meter with 3 × 3 meters of seeding row spacing, the soil that span is equal to by tether panel soil and with tree crown is transplanted to breeding interior；
Tree disk is put in order in spring next year late March, the tree well of 8~10 centimetres of lower digging is standby；Before late April tree rudiment, in tree disk
The nitrogenous aqueous solution that concentration is 750~1000ppm is poured, to promote the growth of sprouting blade, the Second Year warp after transplant survival
Cross dwarf treatment, prune, need determine hat, prune and draw branch in growth course.
Due to cultivating with demand of bearing fruit conversely, making full use of the new numerous slightly for carrying out of apricot sprouting for wild apricot tree
Honeybee.It is divided into and determines hat, pruning, draws the measures such as branch to cultivate.Fixed hat：To wild apricot tree in 1.5 meters or so cuttings of the height of tree, tree crown half
Footpath is controlled at 1.5 meters or so, is easy to connect aphid and is connect honeybee etc. and manually operates.It is tree-like based on the happy shape of cup-shaped, increase branch it is penetrating
Property, it is easy to connect aphid, connects honeybee operation.Prune：Carried out before rudiment after transplanting, each branch of ansu apricot tree is pruned, done
To " lopwood is asked ", branch bounces back to the spring of the previous year slightly, to promote the sprouting of Second Year leaf bud.Draw branch：Sprouted in spring then
After bud, in time by position should not, the excessive close crowded young shoot of quantity or spray erase, to reduce nutrient consumption, make the branch for leaving
Obtain sufficient nutrition and illumination.To the upright branch of vigorous growth, can be pulled into after resin flowing to before rudiment
Certain angle, to relax growing way.Pinching：Position to being sprouted in tree crown is suitable, illumination condition compared with benefit the branch of excessive growth, update
The tip, extension branch, the golden mean of the Confucian school branch of the vertical growth of back, all can pinching, before time of pinching is for resin flowing.
Second Year after transplant survival reaches more than 5 DEG C by dwarf treatment, pruning in ground temperature, and young leaves will by sprouting
Ansu apricot tree after transplanting is covered in numerous honeybee canopy, and temperature control is trained in 10~20 DEG C, the environment of humidity 40~60%, illumination abundance
Educate host plant, when cultivate 7~10 days wild apricot tree young sprouts grow 30~40 centimetres newly slightly when, the 3rd~5 after growing point
The behind of leaf is inoculated with for aphid.
(3) aphid inoculation：The cigarette aphid in field is gathered, is purificated and rejuvenated using tobacco seedlings or Luo Bumiao or Chinese cabbage seedling, prevent viral biography
Broadcast；Can be placed on aphid after detoxification in growth cabinet and cultivate by winter.
Choose the easily parasitic wild apricot of aphid to transplant to room is bred, breed 5,6 years raw wild apricot trees of indoor selection
About 10% number is 6, is cultivated by the epidemic disaster condition of culture of step (2)；When young sprout reaches 30~40 centimetres
Aphid inoculation is carried out on young sprout, collection III ages, IV ages are inoculated into step (2) not by parasitic aptery larvae aphid as aphid worm kind
In new the 3rd~5 leaf position slightly of the centimeters of wild apricot tree 30~40 that cultivate vacuum side of blade, each is newly slightly inoculated with 6~9
Aphid is more suitable, and every plant of ansu apricot tree cigarette aphid is bred to 40000~60000, if making aphid adult be bred on host plant
Worm；The number of aphid amount is connect, also can be short according to honeybee interval depending on putting honeybee interval length, is such as put, but each newly slightly connects aphid amount and is
10~20 heads/plant；If interval is shorter, each newly slightly connects aphid amount for 20~30 heads/plant.
Connect aphid period：After wild apricot is newly slightly sprouted 7~10 days, its kind of aphid source is essentially from peach and greenhouse protecting field
On vegetables, under the conditions of 10~20 DEG C, cigarette aphid completes to develop from generation to generation by only needing 6~7 days.
(4) aphid breeds：During numerous aphid, it is ensured that water, fertilizer supply, nitrogenous fertilizer is supplemented in time, branch blade is kept tender
Green shape, is conducive to the growth of aphis population quantity；By aphid worm kind newly slightly the leaf of 5~10 centimeters the 3rd~5 position blade back
Raising is bred 10 days, and control aphid is postvaccinal to breed indoor temperature control in 10~20 DEG C, humidity 40~60%, makes ansu apricot tree
Cigarette aphid reaches 400~500 on each new slightly branch, and every plant of ansu apricot tree cigarette aphid reaches 40000~60000, is breeding mid-term
Spray a carbendazol to carry out disinfection, obtain aphid parasitism branch.
(5) aphidius gifuensis inoculation is bred (a small amount of)：The aphidius gifuensis in field are gathered in autumn last year, using tobacco seedlings or Chinese cabbage
The parasitic aphid of seedling, aphidius gifuensis (the i.e. parasitic aphid of aphidius gifuensis and tobacco seedlings or Chinese cabbage seedling is put into according to 1: 100 honeybee aphid ratio
Ratio be 1: 100), kind of a honeybee child care is carried out in growth cabinet；
What collection had been sprouted wings carries out artificial infection into honeybee or the stiff aphid that will sprout wings, when each young sprout reaches in step (4)
400~500, when every plant of ansu apricot tree reaches 40000~60000,50% number (3) aphid in step (3) is parasitic
Wild apricot tree, the mesh fly net of outer cover 80~90, choose aphidius gifuensis stiff aphid, reject hyperparasite honeybee, collection sprouted wings into
Honeybee or the stiff aphid that will sprout wings, are put into net in the ratio of honeybee, aphid 1: 30 and are manually connect honeybee, to aphid after aphidius gifuensis are sprouted wings
Worm carries out parasitism.
10~20 DEG C of temperature, the humidity 40~60% of room are bred in control after aphidius gifuensis inoculation, into temperature during bee eclosion
Control is in 15~25 DEG C, humidity 60~80%, while suitably being managed host plant liquid manure cultivation, cultivates 10~15 days shapes
Into a large amount of stiff aphids, honeybee kind parasitism branch is thus obtained；
Honeybee method is connect using stiff aphid, i.e., the stiff aphid that will sprout wings is placed in numerous honeybee canopy, into bee eclosion after voluntarily find cigarette aphid
It is parasitic.Generally, each numerous honeybee hut connects honeybee 9000~15000 or so and is advisable, and aphid parasitic rate living reaches in 2~3 days
More than 80%.This kind of to connect honeybee method, filial generation is concentrated into bee eclosion, is easy to the further expanding propagation of kind of honeybee.
Plant honeybee expanding propagation：Under 15~25 DEG C of constant temperature, aphidius gifuensis complete one needs 12 days or so from generation to generation,
Under field conditions (factors), it is about 15~20 days to the time largely sprouted wings into honeybee from connecing honeybee, thus calculates, can then enters within about 20 days in advance
Row kind honeybee expanding propagation.While numerous aphidius gifuensis are expanded, the culture of host plant should be focused on, increasing connects aphid amount, make its compared with
Substantial amounts of cigarette aphid is bred out in the short time.
(6) aphidius gifuensis Ashmaed scale is bred：By remaining about 90% wild apricot tree of number (50), outer cover in step (3)
80~90 mesh fly nets, training orientation is carried out by the epidemic disaster condition in step (2)；By the CMC model aphid of step (3,4)
Parasitic branch, connecing aphid method is：The aphid parasitism branch (3 aphid parasitism branches of tree) cultivated in selecting step (4) connects
Plant to remaining 50 wild apricot trees and treat on parasitic branch, take and tremble connection i.e. in the vacuum side of blade of new slightly the 3rd~5 leaf position of formation
Gently shaken with the branch of parasitic aphid, it is upper that each branch there are 6~9 aphids to fall, and in dither process, makes III ages, IV ages
Aphid to be fallen carried out on branch and connects aphid；
After connecing aphid 10~12 days, each branch breeding cigarette aphid 400~500 is reached, every plant of wild apricot tree reaches 40000
At~60000, the honeybee kind parasitism branch (3 honeybee kind parasitism branches of tree) cultivated in selecting step (5), according to 1: 30
Honeybee aphid ratio carries out connecing honeybee, to accelerate aphidius gifuensis reproduction speed and breeding amount, when aphidius gifuensis parasitic rate reaches on each branch
80% can put honeybee；
The regulation of temperature of shed, humidity and illumination：Numerous 10~20 DEG C of honeybee canopy temperature, humidity 40 are controlled after aphidius gifuensis inoculation
~55%；Into temperature control during bee eclosion in 15~25 DEG C, humidity 60~80%, while being carried out suitably to host plant liquid manure
Management is cultivated, and is cultivated and is formed within 10~15 days a large amount of deadlock aphids；When carrying out warm Room management, to take into account host plant growth,
Cigarette aphid and the growth of aphidius gifuensis., when 10~20 DEG C, relative humidity are 60~70%, sprout wings due into bee eclosion, survival
Rate is usually 70~80%；And temperature is higher than 27 DEG C or relative humidity when being less than 40%, then eclosion rate is remarkably decreased, therefore into honeybee
, at 18 ± 2 DEG C, humidity more than 60% is conveniently for temperature control during emergence.There is stronger phototaxis and upward property into honeybee in addition,
At the drift angle of the phototropic face that numerous honeybee canopy is combined in into bee eclosion post-concentration, illumination herein is strong, temperature is high, easily makes into honeybee over-activity,
The mortality of honeybee is easily caused into, therefore by installing shade net in canopy, the occurrence of this can be reduced.
Feeding honey：25% G/W and 10% hydromel can also be fed under above-mentioned control condition into honeybee
Preferably, both feeds can extend life span of adult 3~4 days to effect, specific method：5% honey is being speckled with into the suspension of honeybee habitat
The meche or cotton swab of water take food for honeybee, can extend its life-span and increase egg laying amount, improve parasitic effects.
(7) honeybee is put：The branch that stiff aphid will be formed is cut apart from the centimeters of leaf tip 30~40, by with the wild of stiff aphid
Be placed on for the feeding solution of cuttage branch in the nitrogenous fertilizer feeding solution that weight is 5 ‰ by the branch cutting of ansu apricot or peach
In column type bee-escape, the mesh fly net of bee-escape outer cover 50~60 can extend and put the honeybee time, improve prevention effect, every 2 branches
1~2 mu of vega can be prevented and treated, preventive effect can reach more than 75%；After the stiff aphid on branch sprouts wings, flown out from fly net to vega
Host is found, and aphid is blocked in bee-escape；Putting can be used as next time after the aphid remained on the branch after honeybee is withdrawn deposit
Breed the kind aphid of aphid.Bee-escape is made up of the mesh fly net of frame cover 60~80 of cylinder and puts honeybee cage, and feeding solution is consolidated
It is scheduled in the middle of bee-escape, can so exempts tobacco leaf field and put the workload that aphid is rejected before honeybee；Bee-escape top disposes rain-proof
Eaves, has an impact with preventing rain wash to putting honeybee effect；
(8) collection and preservation of aphidius gifuensis：After the aphid nymph that apricot newly slightly goes up in warmhouse booth is changed into stiff aphid, will post
The leaf harvest that main plant has stiff aphid gets off, and removes aphid remaining on blade, obtains stiff aphid blade, and stiff aphid blade is placed on into room
Interior natural air drying 0.5 day, then the stiff aphid on air-dried stiff aphid blade is separated, is fitted into appearance honeybee bottle, and will hold honeybee bottle in 1~
It is stored refrigerated in 3 DEG C of environment.
Largely formed in stiff aphid, stiff aphid branch can be collected when not sprouting wings into honeybee be placed in and put in honeybee canopy in field adaptability, or into
Honeybee should in time collect into honeybee in (must generally being put in 3 hours in field adaptability in vial or sandbag after largely sprouting wings
Honeybee), collected should be In Shade into honeybee, it is to avoid direct sunlight and extruding.Due to some biological habits of hyperparasite honeybee
Similar to aphidius gifuensis, two kinds of honeybees easily mix at one, and honeybee quality is put in influence, therefore, glass was used before aphidius gifuensis are collected
The suction buzzer that bottle makes, honeybee is received after hyperparasite honeybee is rejected again.Putting can be under after the aphid remained on the branch after honeybee is withdrawn deposit
Once breed the kind aphid of aphid.
(the host plant of embodiment 2：Peach seedling)
(1) room construction is bred：Room is bred for steelframe glass structure, 25m long, width 20m, 4m high, construction area 500m2, it is whole
Individual heliogreenhouse is provided with shading screen, 1m aisle wide, using 80~90 mesh fly nets as numerous honeybee canopy separation layer.
(2) host plant cultivating and managing：Breed indoor selection raw peach seedling cuttage in 2~3 years grown in Nutrition Soil it is numerous
Grow, Nutrition Soil composition is seedling medium and vega soil 1: 1 mixture by weight, and the seeding row spacing of cuttage is 10~15 centimetres.Cuttage
After sapling survives, Second Year spring concentration is that the nitrogenous aqueous solution of 750~1000ppm is poured, promote blade to sprout,
Growth；10~20 DEG C of temperature is cultivated, humidity 40~60% cultivates host plant in the suitable environment of illumination abundance humiture,
Cultivate 10, when host plant grows 20~30 centimetres new standby after a while；Once pruned every 30 days, liquid manure is cultivated,
Ensure that whole Growing season has host plant standby.
(3) aphid inoculation：Collection III ages, IV ages are inoculated into step not by parasitic aptery larvae aphid as aphid worm kind
(2) vacuum side of blade of the 3rd~5 leaf position, each peach seedling branch after the new slightly growing point of 20~30 centimetres of the peach seedling cultivated in
Aphid 6~9 is connect, center is dry to reach 15~20, make aphid adult that nymph is bred on host plant, individual plant cigarette aphid reaches 8000
~10000.
(4) aphid breeds：By the new postvaccinal branch in the slightly leaf of blade back the 3rd~5 position of peach seedling or center is dry is raised
Support and breed, aphid is postvaccinal breeds 10~20 DEG C of indoor temperature for control, and humidity 40~60% makes host plant suitable in liquid manure
Under the conditions of breed 10~12 days, individual plant cigarette aphid reaches 8000~10000, breeds mid-term and sprays a carbendazol and is disappeared
Poison.
(5) aphidius gifuensis inoculation：Collection sprouted wings into honeybee or the stiff aphid that will sprout wings, the individual plant cigarette aphid in (4) step
Amount is when reaching 8000~10000, carries out artificial connecing honeybee according to 1: 30 honeybee, aphid ratio.
(6) aphidius gifuensis Ashmaed scale is bred：Room temperature control is bred after aphidius gifuensis inoculation at 10~20 DEG C, humidity 75~
80%, host plant liquid manure suitably manages cultivation, into breeding room temperature control during bee eclosion at 22~27 DEG C, humidity 50~
60%, cultivate and form within 10th a large amount of deadlock aphids.
(7) collection and preservation of aphidius gifuensis：Breed after the aphid nymph in room is changed into stiff aphid, host plant blades are adopted
Collection gets off, and removes aphid remaining on blade, obtains stiff aphid blade, and stiff aphid blade is placed on into Indoor Natural air-dries 2~3 days, will
Stiff aphid on air-dried stiff aphid blade separates, and is fitted into appearance honeybee bottle, holds honeybee bottle and is stored in 3~5 DEG C of refrigerating chamber.
Embodiment 2 other do not repeat embodiment 1 technology contents with embodiment 1.
Claims (6)
1. a kind of method of quick breeding aphidius gifuensis, it is characterised in that：Cultivated including host plant, worm kind is bred, honeybee kind is numerous
Educate and bred with aphidius gifuensis, the host plant of selection is tall graminaceous plant wild apricot tree or peach seedling, after carrying out liquid manure culture,
The young tender of its pumping newly slightly carries out numerous honeybee, specifically breeds including following method：
(1) host plant is cultivated：The tall graminaceous plant of the strong no disease and pests harm of selection, its age of tree 5~6 years wild apricot tree, 2~
Raw peach seedling is transplanted within 3 years, and Second Year spring concentration is that the nitrogenous aqueous solution of 750~1000ppm is poured, and promotes leaf
Piece is sprouted, growth；Second Year after transplant survival is cultivated by dwarf treatment, pruning and liquid manure, and wild apricot tree was growing
Cheng Zhongxu is carried out determining hat, pruning, pinching and is drawn branch；
Reach more than 5 DEG C in ground temperature, young leaves sprout after by the host plant after transplanting is covered in the numerous honeybee canopy bred in room,
In 10~20 DEG C of temperature, humidity 40~60% cultivates host plant under the sufficient environment of illumination, is planted when 7~15 days hosts are cultivated
Thing grow 10~40 centimetres it is new slightly when, the 3rd~5 leaf is inoculated with for aphid after growing point；
(2) worm kind is bred：The cigarette aphid in field is gathered, is purificated and rejuvenated using tobacco seedlings or Luo Bumiao or Chinese cabbage seedling, prevent viral transmission；
Can be placed on aphid after detoxification in growth cabinet and cultivate standby by winter；
The easily parasitic wild apricot tree of aphid or peach transplantation of seedlings are chosen to after breeding room, indoor selection host plant 10 is being bred
~15% strain number, is cultivated by the epidemic disaster condition in step (1)；When the growth of host plant young sprout reaches predetermined length
At 10~40 centimetres, collection III ages, IV ages are inoculated into cultivate wild as aphid worm kind not by parasitic aptery larvae aphid
The vacuum side of blade of the 3rd~5 leaf position after ansu apricot tree or the new slightly growing point of 10~40 centimetres of peach seedling, each is newly slightly inoculated with 6~9 aphids
Worm, to 40000~60000, aphid adult breeds standby during nymph on host plant for every plant of tree cigarette aphid breeding；
By aphid worm kind, the vacuum side of blade raising of the 3rd~5 leaf position is bred 7~12 after growing point, and control aphid is postvaccinal
10~25 DEG C of numerous honeybee room temperature, humidity 40~60% makes cigarette aphid on wild apricot tree each new slightly branch reach 400~500,
Every plant of upper cigarette aphid of tree reaches 40000~60000, and peach seedling individual plant cigarette aphid reaches 8000~10000, breeds mid-term and sprays one
Secondary carbendazol carries out disinfection, and obtains aphid parasitism branch；
(3) honeybee kind is bred：The aphidius gifuensis in field are gathered in autumn last year, using tobacco seedlings or the parasitic aphid of Chinese cabbage seedling, according to 1
: 100 honeybee aphid ratio is put into aphidius gifuensis, and kind of a honeybee child care is carried out in growth cabinet；
The wild apricot tree of 50~60% numbers that step (2) is obtained or the aphid parasitism branch of peach seedling, outer cover 80~90
Mesh fly net, chooses the stiff aphid of aphidius gifuensis, rejects hyperparasite honeybee, and what collection had been sprouted wings is carried out into honeybee or the stiff aphid that will sprout wings
Artificial infection, when wild apricot tree, each new slightly inoculation reaches 400~500, and every plant is set up to 40000~60000, peach seedling
When individual plant cigarette aphid reaches 8000~10000, it is put into net by 1: 25~30 honeybee, aphid ratio and is manually connect honeybee, treats cigarette aphid cocoon
Parasitism is carried out to aphid after bee eclosion；
10~25 DEG C of the temperature of room, humidity 30~60%, into bee eclosion are bred in the control after aphidius gifuensis inoculation of wild apricot tree
Period temperature control is in 15~25 DEG C, humidity 60~80%；Peach seedling temperature of shed after aphidius gifuensis inoculation is 10~20 DEG C,
Humidity 70~80%, is 22~30 DEG C, humidity 50~60% into temperature during bee eclosion；Host plant liquid manure is fitted simultaneously
Cultivation is preferably managed, is cultivated and is formed within 7~15 days a large amount of deadlock aphids, thus obtain honeybee kind parasitism branch；
(4) aphidius gifuensis Ashmaed scale is bred：By remaining 90~85% the wild apricot trees or peach seedling of number, outer cover in step (2)
80~90 mesh fly nets, training orientation is carried out by the epidemic disaster condition in step (1)；Posted by the CMC model aphid of step (2)
Raw branch：The wild apricot tree or peach seedling that the aphid parasitism branch cultivated in selecting step (2) is inoculated into remainder are treated
On parasitic branch, take and tremble connection and gently trembled in the branch of the parasitic aphid of vacuum side of blade for forming new slightly the 3rd~5 leaf position
Dynamic, it is upper that each branch has 6~9 aphids to fall, and in dither process, III ages, IV age aphids is fallen and connect on branch
Aphid；
After connecing aphid 10~12 days, wild apricot tree reaches each branch breeding cigarette aphid 400~500, every plant 40000~60000
Head, when peach seedling individual plant cigarette aphid reaches 8000~10000, the honeybee kind parasitism branch cultivated in selecting step (3), according to 1:
25~30 honeybee aphid ratio carries out connecing honeybee, when on each branch aphidius gifuensis parasitic rate reach 70~80% by put honeybee；
(5) honeybee is put：The branch that stiff aphid will be formed is cut apart from the centimeters of leaf tip 30~40, ansu apricot or peach with stiff aphid
Be placed in the reservoir of cuttage branch in column type bee-escape in the nitrogenous fertilizer reservoir that weight is 5 ‰ by branch cutting,
The mesh fly net of bee-escape outer cover 50~60, after the stiff aphid on branch sprouts wings, flies out to vega from fly net and finds host, aphid
Worm is blocked in bee-escape；Putting can be used as the kind aphid for breeding aphid next time after the aphid remained on the branch after honeybee is withdrawn deposit；
(6) collection and preservation of aphidius gifuensis：After apricot or the new aphid nymph for slightly going up of peach are changed into stiff aphid, by host plant band
The leaf harvest for having stiff aphid gets off, and removes aphid remaining on blade, obtains stiff aphid blade, and stiff aphid blade is placed on into Indoor Natural
Air-dry 0.5~3 day, then the stiff aphid on air-dried stiff aphid blade is separated, be fitted into appearance honeybee bottle, and honeybee bottle will be held in 1~5 DEG C
It is stored refrigerated in environment.
2. the method for quick breeding aphidius gifuensis according to claim 1, it is characterised in that：Described wild apricot tree
Growth temperature is 10~20 DEG C, humidity 40~60%, is cultivated in the sufficient environment of illumination, wild when cultivating 7~15 days
Ansu apricot tree young sprout implements aphid inoculation when being up to 30~40 centimetres.
3. the method for quick breeding aphidius gifuensis according to claim 1, it is characterised in that：Described wild apricot tree sprouts
The new of hair is slightly used for carrying out numerous honeybee, by determining hat, pruning, drawing branch and pinching to be cultivated, fixed hat：To wild apricot tree in the height of tree
1.5 meters or so cuttings, tree crown radius is controlled at 1.5 meters, tree-like for ease of connecing aphid, connecing honeybee operation, increases the cup of branch permeability
The happy shape of shape；Prune：Carried out before rudiment after transplanting, each branch of wild apricot tree is pruned, branch is bounced back to
The spring of the previous year slightly, with promote Second Year leaf bud sprout；Draw branch：After spring rudiment then, in time by position is unsuitable, quantity mistake
The close crowded young shoot or spray of many consumption nutrition are erased, and the branch for leaving is obtained sufficient nutrition and illumination, to vigorous growth
Upright branch, to before rudiment after the resin flowing, is pulled into the angle for relaxing growing way；Pinching：To what is sprouted in tree crown
Position is suitable, illumination condition carries out pinching compared with the golden mean of the Confucian school branch of the branch of excessive growth of benefit, more young sprout, extension branch, back vertical growth, plucks
The heart time is before resin flows.
4. the method for quick breeding aphidius gifuensis according to claim 1, it is characterised in that：Described wild apricot tree exists
10~20 DEG C of numerous honeybee temperature of shed is controlled after aphidius gifuensis inoculation, humidity 40~55% is 15~25 into temperature during bee eclosion
DEG C, humidity 60~80%；Peach seedling temperature of shed after aphidius gifuensis inoculation is 10~20 DEG C, humidity 75~80%, into honeybee plumage
22~27 DEG C of temperature during change, humidity 50~60%.
5. the method for quick breeding aphidius gifuensis according to claim 1, it is characterised in that：Selection the age of tree 5~6 years,
The tall graminaceous plant wild apricot tree of healthy and strong no disease and pests harm, in late October last year~early November, by tether panel soil and with tree
The soil of the equivalent span of hat is transplanted to interior is bred, and tree disk, the tree of 8~10 centimetres of lower digging are put in order in spring next year late March
Hole is standby；Before late April tree rudiment, the nitrogenous aqueous solution that concentration is 750~1000ppm is poured in tree disk, promote what is sprouted
Leaf growth；Second Year after transplant survival by dwarf treatment, prune, need to carry out in growth course to determine hat, pruning, pinching and
Draw branch.
6. the method for quick breeding aphidius gifuensis according to claim 1, it is characterised in that：Described host plant selection
2~3 years raw peach seedling cuttage growth and breedings in Nutrition Soil, Nutrition Soil composition is seedling medium and vega soil by weight 1: 1
Mixture, 10~15 centimetres of cuttage seeding row spacing；Second Year spring concentration after surviving is the nitrogenous water-soluble of 750~1000ppm
Liquid is poured, and promotes blade to sprout, grow；10~20 DEG C of temperature is cultivated, humidity 40~60% is cultivated in the sufficient environment of illumination
Host plant, cultivates 10, when peach seedling grows 20~30 centimetres new standby after a while；Once pruned every 30 days, water
Fertilizer is cultivated, it is ensured that whole Growing season has host plant standby.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611156232.1A CN106689066A (en) | 2016-12-08 | 2016-12-08 | Method for quickly breeding aphidius gifuensis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611156232.1A CN106689066A (en) | 2016-12-08 | 2016-12-08 | Method for quickly breeding aphidius gifuensis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106689066A true CN106689066A (en) | 2017-05-24 |
Family
ID=58937669
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611156232.1A Pending CN106689066A (en) | 2016-12-08 | 2016-12-08 | Method for quickly breeding aphidius gifuensis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106689066A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107296015A (en) * | 2017-08-23 | 2017-10-27 | 云南省烟草公司红河州公司 | A kind of aphidius gifuensis product industrial production method |
| CN108207822A (en) * | 2018-03-12 | 2018-06-29 | 内蒙古农业大学 | A kind of artificial a large amount of fast breeding technical methods of brown band aphid chalcid fly |
| CN109221022A (en) * | 2018-10-29 | 2019-01-18 | 云南省烟草公司昆明市公司 | Aphid parasite product automation process units |
| CN110122429A (en) * | 2019-06-18 | 2019-08-16 | 河南省农业科学院烟草研究所 | A kind of aphid parasite deadlock aphid preservation test method |
| CN111406713A (en) * | 2019-01-08 | 2020-07-14 | 中国科学院动物研究所 | Method for breeding parasitic wasps on large scale |
| CN112385608A (en) * | 2019-08-19 | 2021-02-23 | 云南省烟草公司大理州公司 | Breeding method of lugworm |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103478083A (en) * | 2013-09-29 | 2014-01-01 | 任伟 | Large-scale breeding method for breeding mummified aphids and adult bees of aphidiusgifuensis by yellow-green myzuspersicae |
| CN104585135A (en) * | 2014-12-21 | 2015-05-06 | 辽宁省烟草公司朝阳市公司 | Overwintering breeding method for aphidiusgifuensis in northern area |
-
2016
- 2016-12-08 CN CN201611156232.1A patent/CN106689066A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103478083A (en) * | 2013-09-29 | 2014-01-01 | 任伟 | Large-scale breeding method for breeding mummified aphids and adult bees of aphidiusgifuensis by yellow-green myzuspersicae |
| CN104585135A (en) * | 2014-12-21 | 2015-05-06 | 辽宁省烟草公司朝阳市公司 | Overwintering breeding method for aphidiusgifuensis in northern area |
Non-Patent Citations (2)
| Title |
|---|
| 张克俊: "《果树整形修剪技术问答》", 30 June 2000, 中国农业出版社 * |
| 韩金声: "《植物医院实用技术指南》", 30 September 1996, 中国农业大学出版社 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107296015A (en) * | 2017-08-23 | 2017-10-27 | 云南省烟草公司红河州公司 | A kind of aphidius gifuensis product industrial production method |
| CN108207822A (en) * | 2018-03-12 | 2018-06-29 | 内蒙古农业大学 | A kind of artificial a large amount of fast breeding technical methods of brown band aphid chalcid fly |
| CN108207822B (en) * | 2018-03-12 | 2020-10-02 | 内蒙古农业大学 | Artificial mass rapid breeding technical method for Aphidius fusceolatus |
| CN109221022A (en) * | 2018-10-29 | 2019-01-18 | 云南省烟草公司昆明市公司 | Aphid parasite product automation process units |
| CN109221022B (en) * | 2018-10-29 | 2023-09-26 | 云南省烟草公司昆明市公司 | Automatic aphidiidae product production device |
| CN111406713A (en) * | 2019-01-08 | 2020-07-14 | 中国科学院动物研究所 | Method for breeding parasitic wasps on large scale |
| CN111406713B (en) * | 2019-01-08 | 2021-08-24 | 中国科学院动物研究所 | Method for breeding parasitic wasps on large scale |
| CN110122429A (en) * | 2019-06-18 | 2019-08-16 | 河南省农业科学院烟草研究所 | A kind of aphid parasite deadlock aphid preservation test method |
| CN110122429B (en) * | 2019-06-18 | 2021-07-02 | 河南省农业科学院烟草研究所 | Stiff aphid preservation test method for aphidiidae |
| CN112385608A (en) * | 2019-08-19 | 2021-02-23 | 云南省烟草公司大理州公司 | Breeding method of lugworm |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106689066A (en) | Method for quickly breeding aphidius gifuensis | |
| CN102301982B (en) | Method for propagating Aphidius gifuensis by pea aphids | |
| CN104585135A (en) | Overwintering breeding method for aphidiusgifuensis in northern area | |
| CN104365465B (en) | The interplanting device and its cultural method of Herba Dendrobii and Herba Anoectochili roxburghii | |
| CN105638362A (en) | Cultivation method for interplanting cherry trees in tea garden | |
| CN103798010B (en) | A kind ofly santal is promoted early to become a useful person and the cultivation method of high yield | |
| CN104996111A (en) | Dendrobium officinale cultivation method | |
| CN101185394A (en) | High quality high-efficiency cultivation method for imperial crown watermelon | |
| CN109328891A (en) | A kind of method of eucalyptus underwood planting Baphicanthus cusia | |
| CN106818378A (en) | Orchard pest ecological control method and satsuma orange cultural method based on " push-and-pull " strategy | |
| CN104025830A (en) | Greenhouse cherry cultivation method | |
| CN103583340A (en) | Gardenia multi-branch floating bed water planting cuttage method | |
| CN104718938A (en) | Tobacco planting method combining half-dry land bed seedling and warm-hole type transplanting | |
| CN104081990A (en) | Method for stereoscopic planting of vegetables in greenhouse | |
| CN108887124A (en) | A kind of Wild gastrodia artificial cultivation method | |
| CN103918459A (en) | Method for cutting propagation of cephalomappa sinensis | |
| CN104718968B (en) | The artificial cultivation method of Rubus chingii | |
| CN1522561A (en) | Potted loquat and its cultivation method | |
| CN104206140B (en) | The cultural method of gold silver grass 2 | |
| CN109757274B (en) | High-yield and high-efficiency transplanting method for momordica grosvenori tissue culture seedlings | |
| CN102450151A (en) | Technology for planting Huangpi watermelon | |
| CN102792831B (en) | High-efficiency rapid propagation technique for Chinese yew | |
| CN111357574A (en) | High-yield planting method of Epimedium sagittatum | |
| CN105660190A (en) | Stereoscopic cultivation and breeding method for complementary symbiosis of ganoderma lucidum and wood frogs | |
| CN104663398A (en) | Water mist cultivation method for melon vine type vegetables and fruits |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170524 |
|
| RJ01 | Rejection of invention patent application after publication |