CN107356515A - 一种荧光图像的荧光强度确定方法和*** - Google Patents
一种荧光图像的荧光强度确定方法和*** Download PDFInfo
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- CN107356515A CN107356515A CN201710596449.2A CN201710596449A CN107356515A CN 107356515 A CN107356515 A CN 107356515A CN 201710596449 A CN201710596449 A CN 201710596449A CN 107356515 A CN107356515 A CN 107356515A
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- 238000000034 method Methods 0.000 title claims abstract description 49
- 238000001514 detection method Methods 0.000 claims abstract description 86
- 238000007621 cluster analysis Methods 0.000 claims abstract description 32
- 238000003384 imaging method Methods 0.000 claims abstract description 25
- 238000004458 analytical method Methods 0.000 claims abstract description 16
- 230000011218 segmentation Effects 0.000 claims abstract description 13
- 238000000799 fluorescence microscopy Methods 0.000 claims abstract description 10
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- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
- G06V20/00—Scenes; Scene-specific elements
- G06V20/60—Type of objects
- G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1429—Signal processing
- G01N15/1431—Signal processing the electronics being integrated with the analyser, e.g. hand-held devices for on-site investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1429—Signal processing
- G01N15/1433—Signal processing using image recognition
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1434—Optical arrangements
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1434—Optical arrangements
- G01N2015/144—Imaging characterised by its optical setup
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N2015/1493—Particle size
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- General Physics & Mathematics (AREA)
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- Biomedical Technology (AREA)
- Molecular Biology (AREA)
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- Theoretical Computer Science (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Computer Vision & Pattern Recognition (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
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Abstract
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CN201710596449.2A CN107356515B (zh) | 2017-07-20 | 2017-07-20 | 一种荧光图像的荧光强度确定方法和*** |
PCT/CN2018/095239 WO2019015514A1 (zh) | 2017-07-20 | 2018-07-11 | 一种荧光图像的荧光强度确定方法和*** |
JP2020523477A JP6851670B2 (ja) | 2017-07-20 | 2018-07-11 | 蛍光画像の蛍光強度確定方法及びシステム |
EP18835191.0A EP3657154A4 (en) | 2017-07-20 | 2018-07-11 | METHOD AND SYSTEM FOR DETERMINING THE FLUORESCENT INTENSITY OF A FLUORESCENT IMAGE |
US16/629,400 US11249005B2 (en) | 2017-07-20 | 2018-07-11 | Method and system for determining fluorescence intensity of fluorescence image |
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CN201710596449.2A CN107356515B (zh) | 2017-07-20 | 2017-07-20 | 一种荧光图像的荧光强度确定方法和*** |
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CN107356515A true CN107356515A (zh) | 2017-11-17 |
CN107356515B CN107356515B (zh) | 2020-04-03 |
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CN201710596449.2A Active CN107356515B (zh) | 2017-07-20 | 2017-07-20 | 一种荧光图像的荧光强度确定方法和*** |
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US (1) | US11249005B2 (zh) |
EP (1) | EP3657154A4 (zh) |
JP (1) | JP6851670B2 (zh) |
CN (1) | CN107356515B (zh) |
WO (1) | WO2019015514A1 (zh) |
Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2019015514A1 (zh) * | 2017-07-20 | 2019-01-24 | 上海睿钰生物科技有限公司 | 一种荧光图像的荧光强度确定方法和*** |
CN109800631A (zh) * | 2018-12-07 | 2019-05-24 | 天津大学 | 基于掩膜区域卷积神经网络的荧光编码微球图像检测方法 |
CN109815870A (zh) * | 2019-01-17 | 2019-05-28 | 华中科技大学 | 细胞表型图像定量分析的高通量功能基因筛选方法及*** |
CN110009619A (zh) * | 2019-04-02 | 2019-07-12 | 清华大学深圳研究生院 | 一种基于荧光编码的液相生物芯片的图像分析方法 |
CN111289484A (zh) * | 2020-03-11 | 2020-06-16 | 哈尔滨工业大学(威海) | 一种基于罗丹明b荧光特性的冷表皮检测方法 |
CN112819795A (zh) * | 2021-02-05 | 2021-05-18 | 复旦大学附属中山医院 | 基于多色荧光图片的细胞数量和面积的统计方法 |
CN113237818A (zh) * | 2021-05-28 | 2021-08-10 | 上海睿钰生物科技有限公司 | 一种细胞分析方法和*** |
CN116124754A (zh) * | 2023-04-17 | 2023-05-16 | 山东省中源联科生物工程集团有限公司 | 通过图像分析检测透明质酸钠凝胶中交联剂残留量的方法 |
CN116519650A (zh) * | 2023-04-17 | 2023-08-01 | 北京大学口腔医学院 | 一种骨组织结构状态检测方法 |
Families Citing this family (3)
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US20220236164A1 (en) * | 2021-01-25 | 2022-07-28 | Becton, Dickinson And Company | Method and systems for determing drop delay using scatter signals across spatially separated lasers |
CN112907582B (zh) * | 2021-03-24 | 2023-09-29 | 中国矿业大学 | 面向矿井的图像显著性提取去雾方法、装置及人脸检测 |
CN113450279B (zh) * | 2021-07-01 | 2023-03-14 | 维柯基科技(上海)有限公司 | 一种多孔荧光微阵列图像的荧光强度检测方法、装置、计算机设备及计算机可读存储介质 |
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CN104266956A (zh) * | 2014-09-02 | 2015-01-07 | 上海凯度机电科技有限公司 | 高内涵图像流式生物显微分析方法 |
US20150024385A1 (en) * | 2013-07-22 | 2015-01-22 | Wisconsin Alumni Research Foundation | Prediction of fertility in males |
CN105092550A (zh) * | 2015-07-01 | 2015-11-25 | 上海睿钰生物科技有限公司 | 一种荧光显微成像方法和装置 |
CN105928921A (zh) * | 2016-06-07 | 2016-09-07 | 河南科技大学 | 一种通过荧光染色图片数字化进行细胞pH值计算的方法 |
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JP3411115B2 (ja) * | 1994-12-26 | 2003-05-26 | シスメックス株式会社 | フローサイトメータ |
DE10218706B4 (de) * | 2002-04-26 | 2008-02-28 | Leica Microsystems Cms Gmbh | Verfahren zur zeitoptimierten Erfassung von speziellen Spektren mit einem Scanmikroskop |
JP2005315862A (ja) * | 2004-03-30 | 2005-11-10 | Sysmex Corp | 子宮頸癌のスクリーニング方法及び子宮頸癌診断薬 |
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EP1844426A4 (en) * | 2005-02-01 | 2016-09-07 | Amnis Corp | BLOOD ANALYSIS BY MEANS OF FLOW IMAGING CYTOMETER |
WO2011099565A1 (ja) * | 2010-02-10 | 2011-08-18 | シスメックス株式会社 | 血液中癌細胞の検出方法及びそれに用いるプログラム |
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CN107356515B (zh) | 2017-07-20 | 2020-04-03 | 上海睿钰生物科技有限公司 | 一种荧光图像的荧光强度确定方法和*** |
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- 2017-07-20 CN CN201710596449.2A patent/CN107356515B/zh active Active
-
2018
- 2018-07-11 WO PCT/CN2018/095239 patent/WO2019015514A1/zh unknown
- 2018-07-11 JP JP2020523477A patent/JP6851670B2/ja active Active
- 2018-07-11 EP EP18835191.0A patent/EP3657154A4/en active Pending
- 2018-07-11 US US16/629,400 patent/US11249005B2/en active Active
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CN105092550A (zh) * | 2015-07-01 | 2015-11-25 | 上海睿钰生物科技有限公司 | 一种荧光显微成像方法和装置 |
CN105928921A (zh) * | 2016-06-07 | 2016-09-07 | 河南科技大学 | 一种通过荧光染色图片数字化进行细胞pH值计算的方法 |
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Cited By (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2019015514A1 (zh) * | 2017-07-20 | 2019-01-24 | 上海睿钰生物科技有限公司 | 一种荧光图像的荧光强度确定方法和*** |
US11249005B2 (en) | 2017-07-20 | 2022-02-15 | Shanghai Ruiyu Biotech Co., Ltd. | Method and system for determining fluorescence intensity of fluorescence image |
CN109800631B (zh) * | 2018-12-07 | 2023-10-24 | 天津大学 | 基于掩膜区域卷积神经网络的荧光编码微球图像检测方法 |
CN109800631A (zh) * | 2018-12-07 | 2019-05-24 | 天津大学 | 基于掩膜区域卷积神经网络的荧光编码微球图像检测方法 |
CN109815870A (zh) * | 2019-01-17 | 2019-05-28 | 华中科技大学 | 细胞表型图像定量分析的高通量功能基因筛选方法及*** |
CN110009619A (zh) * | 2019-04-02 | 2019-07-12 | 清华大学深圳研究生院 | 一种基于荧光编码的液相生物芯片的图像分析方法 |
CN111289484A (zh) * | 2020-03-11 | 2020-06-16 | 哈尔滨工业大学(威海) | 一种基于罗丹明b荧光特性的冷表皮检测方法 |
CN112819795A (zh) * | 2021-02-05 | 2021-05-18 | 复旦大学附属中山医院 | 基于多色荧光图片的细胞数量和面积的统计方法 |
CN112819795B (zh) * | 2021-02-05 | 2023-03-31 | 复旦大学附属中山医院 | 基于多色荧光图片的细胞数量和面积的统计方法 |
CN113237818A (zh) * | 2021-05-28 | 2021-08-10 | 上海睿钰生物科技有限公司 | 一种细胞分析方法和*** |
CN116519650A (zh) * | 2023-04-17 | 2023-08-01 | 北京大学口腔医学院 | 一种骨组织结构状态检测方法 |
CN116124754A (zh) * | 2023-04-17 | 2023-05-16 | 山东省中源联科生物工程集团有限公司 | 通过图像分析检测透明质酸钠凝胶中交联剂残留量的方法 |
CN116519650B (zh) * | 2023-04-17 | 2024-03-12 | 北京大学口腔医学院 | 一种骨组织结构状态检测方法 |
Also Published As
Publication number | Publication date |
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EP3657154A1 (en) | 2020-05-27 |
CN107356515B (zh) | 2020-04-03 |
WO2019015514A1 (zh) | 2019-01-24 |
US11249005B2 (en) | 2022-02-15 |
JP6851670B2 (ja) | 2021-03-31 |
US20200141856A1 (en) | 2020-05-07 |
EP3657154A4 (en) | 2021-04-21 |
JP2020527274A (ja) | 2020-09-03 |
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