CN107353201B - 一种高含量天然莽草酸提取物及其制备方法 - Google Patents
一种高含量天然莽草酸提取物及其制备方法 Download PDFInfo
- Publication number
- CN107353201B CN107353201B CN201610300286.4A CN201610300286A CN107353201B CN 107353201 B CN107353201 B CN 107353201B CN 201610300286 A CN201610300286 A CN 201610300286A CN 107353201 B CN107353201 B CN 107353201B
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- Prior art keywords
- shikimic acid
- solution
- extract
- exchange resin
- concentrated solution
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- JXOHGGNKMLTUBP-HSUXUTPPSA-N shikimic acid Chemical compound O[C@@H]1CC(C(O)=O)=C[C@@H](O)[C@H]1O JXOHGGNKMLTUBP-HSUXUTPPSA-N 0.000 title claims abstract description 166
- JXOHGGNKMLTUBP-JKUQZMGJSA-N shikimic acid Natural products O[C@@H]1CC(C(O)=O)=C[C@H](O)[C@@H]1O JXOHGGNKMLTUBP-JKUQZMGJSA-N 0.000 title claims abstract description 166
- 239000000284 extract Substances 0.000 title claims abstract description 41
- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- 238000000605 extraction Methods 0.000 title description 8
- 238000004519 manufacturing process Methods 0.000 claims abstract description 46
- 239000002351 wastewater Substances 0.000 claims abstract description 41
- 239000009429 Ginkgo biloba extract Substances 0.000 claims abstract description 40
- 239000003957 anion exchange resin Substances 0.000 claims abstract description 24
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 claims abstract description 18
- 239000003729 cation exchange resin Substances 0.000 claims abstract description 17
- 239000002994 raw material Substances 0.000 claims abstract description 9
- 238000001953 recrystallisation Methods 0.000 claims abstract description 6
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims abstract description 5
- 229910052708 sodium Inorganic materials 0.000 claims abstract description 5
- 239000011734 sodium Substances 0.000 claims abstract description 5
- 239000000243 solution Substances 0.000 claims description 137
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 75
- 238000010828 elution Methods 0.000 claims description 37
- 238000005406 washing Methods 0.000 claims description 34
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 32
- 239000007864 aqueous solution Substances 0.000 claims description 31
- 238000001914 filtration Methods 0.000 claims description 29
- 239000002244 precipitate Substances 0.000 claims description 29
- 238000001035 drying Methods 0.000 claims description 26
- 238000000034 method Methods 0.000 claims description 26
- 238000011068 loading method Methods 0.000 claims description 25
- 239000013078 crystal Substances 0.000 claims description 20
- 239000007788 liquid Substances 0.000 claims description 15
- 239000011347 resin Substances 0.000 claims description 14
- 229920005989 resin Polymers 0.000 claims description 14
- 239000012535 impurity Substances 0.000 claims description 13
- 239000013049 sediment Substances 0.000 claims description 12
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims description 11
- 238000000926 separation method Methods 0.000 claims description 9
- 229910001415 sodium ion Inorganic materials 0.000 claims description 9
- 238000003916 acid precipitation Methods 0.000 claims description 4
- 239000002253 acid Substances 0.000 claims description 3
- 238000001179 sorption measurement Methods 0.000 claims description 3
- PPBRXRYQALVLMV-UHFFFAOYSA-N Styrene Chemical compound C=CC1=CC=CC=C1 PPBRXRYQALVLMV-UHFFFAOYSA-N 0.000 claims 2
- NIXOWILDQLNWCW-UHFFFAOYSA-M Acrylate Chemical group [O-]C(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-M 0.000 claims 1
- 241000218628 Ginkgo Species 0.000 abstract description 17
- 235000011201 Ginkgo Nutrition 0.000 abstract description 17
- 235000008100 Ginkgo biloba Nutrition 0.000 abstract description 17
- 239000003480 eluent Substances 0.000 abstract description 5
- 238000005185 salting out Methods 0.000 abstract 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 18
- 238000012546 transfer Methods 0.000 description 17
- 239000000047 product Substances 0.000 description 11
- 239000000126 substance Substances 0.000 description 11
- 240000007232 Illicium verum Species 0.000 description 10
- 235000008227 Illicium verum Nutrition 0.000 description 10
- 230000000694 effects Effects 0.000 description 9
- 239000012141 concentrate Substances 0.000 description 8
- 235000008504 concentrate Nutrition 0.000 description 8
- 238000011835 investigation Methods 0.000 description 8
- 239000002904 solvent Substances 0.000 description 8
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 6
- 238000000746 purification Methods 0.000 description 6
- 238000011160 research Methods 0.000 description 6
- 229940068052 ginkgo biloba extract Drugs 0.000 description 5
- 235000020686 ginkgo biloba extract Nutrition 0.000 description 5
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 4
- 241000018650 Pinus massoniana Species 0.000 description 4
- 235000011609 Pinus massoniana Nutrition 0.000 description 4
- 239000000706 filtrate Substances 0.000 description 4
- 150000007524 organic acids Chemical class 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 238000012216 screening Methods 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- 241000208682 Liquidambar Species 0.000 description 3
- 235000006552 Liquidambar styraciflua Nutrition 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 238000003786 synthesis reaction Methods 0.000 description 3
- YQUVCSBJEUQKSH-UHFFFAOYSA-N 3,4-dihydroxybenzoic acid Chemical compound OC(=O)C1=CC=C(O)C(O)=C1 YQUVCSBJEUQKSH-UHFFFAOYSA-N 0.000 description 2
- FJKROLUGYXJWQN-UHFFFAOYSA-N 4-hydroxybenzoic acid Chemical compound OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 description 2
- 240000004760 Pimpinella anisum Species 0.000 description 2
- 235000012550 Pimpinella anisum Nutrition 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 239000012043 crude product Substances 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 150000002016 disaccharides Chemical class 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 238000003912 environmental pollution Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- LNTHITQWFMADLM-UHFFFAOYSA-N gallic acid Chemical compound OC(=O)C1=CC(O)=C(O)C(O)=C1 LNTHITQWFMADLM-UHFFFAOYSA-N 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 229920002521 macromolecule Polymers 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 150000002772 monosaccharides Chemical class 0.000 description 2
- 235000005985 organic acids Nutrition 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000007858 starting material Substances 0.000 description 2
- 239000006188 syrup Substances 0.000 description 2
- 235000020357 syrup Nutrition 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 238000003809 water extraction Methods 0.000 description 2
- 229940090248 4-hydroxybenzoic acid Drugs 0.000 description 1
- AEUAEICGCMSYCQ-UHFFFAOYSA-N 4-n-(7-chloroquinolin-1-ium-4-yl)-1-n,1-n-diethylpentane-1,4-diamine;dihydrogen phosphate Chemical compound OP(O)(O)=O.ClC1=CC=C2C(NC(C)CCCN(CC)CC)=CC=NC2=C1 AEUAEICGCMSYCQ-UHFFFAOYSA-N 0.000 description 1
- 241000208140 Acer Species 0.000 description 1
- 230000005526 G1 to G0 transition Effects 0.000 description 1
- 208000002979 Influenza in Birds Diseases 0.000 description 1
- 235000007265 Myrrhis odorata Nutrition 0.000 description 1
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 1
- 241000018646 Pinus brutia Species 0.000 description 1
- 235000011613 Pinus brutia Nutrition 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 238000003811 acetone extraction Methods 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- 150000003797 alkaloid derivatives Chemical class 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 150000004982 aromatic amines Chemical class 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 206010064097 avian influenza Diseases 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- WORJEOGGNQDSOE-UHFFFAOYSA-N chloroform;methanol Chemical compound OC.ClC(Cl)Cl WORJEOGGNQDSOE-UHFFFAOYSA-N 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 239000012156 elution solvent Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 235000004515 gallic acid Nutrition 0.000 description 1
- 229940074391 gallic acid Drugs 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 239000003456 ion exchange resin Substances 0.000 description 1
- 229920003303 ion-exchange polymer Polymers 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- PGZUMBJQJWIWGJ-ONAKXNSWSA-N oseltamivir phosphate Chemical compound OP(O)(O)=O.CCOC(=O)C1=C[C@@H](OC(CC)CC)[C@H](NC(C)=O)[C@@H](N)C1 PGZUMBJQJWIWGJ-ONAKXNSWSA-N 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 238000011028 process validation Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000000284 resting effect Effects 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 238000005063 solubilization Methods 0.000 description 1
- 230000007928 solubilization Effects 0.000 description 1
- 230000003381 solubilizing effect Effects 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 229940061367 tamiflu Drugs 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C51/00—Preparation of carboxylic acids or their salts, halides or anhydrides
- C07C51/42—Separation; Purification; Stabilisation; Use of additives
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C51/00—Preparation of carboxylic acids or their salts, halides or anhydrides
- C07C51/42—Separation; Purification; Stabilisation; Use of additives
- C07C51/43—Separation; Purification; Stabilisation; Use of additives by change of the physical state, e.g. crystallisation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C51/00—Preparation of carboxylic acids or their salts, halides or anhydrides
- C07C51/42—Separation; Purification; Stabilisation; Use of additives
- C07C51/47—Separation; Purification; Stabilisation; Use of additives by solid-liquid treatment; by chemisorption
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Crystallography & Structural Chemistry (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Extraction Or Liquid Replacement (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
Description
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CN201610300286.4A CN107353201B (zh) | 2016-05-09 | 2016-05-09 | 一种高含量天然莽草酸提取物及其制备方法 |
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CN201610300286.4A CN107353201B (zh) | 2016-05-09 | 2016-05-09 | 一种高含量天然莽草酸提取物及其制备方法 |
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CN107353201A CN107353201A (zh) | 2017-11-17 |
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Families Citing this family (13)
Publication number | Priority date | Publication date | Assignee | Title |
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CN108191639A (zh) * | 2018-01-29 | 2018-06-22 | 万邦德制药集团股份有限公司 | 一种从银杏叶提取物层析废液中制备莽草酸提取物的方法 |
CN109593034B (zh) * | 2018-11-22 | 2022-02-11 | 桂林莱茵生物科技股份有限公司 | 一种从银杏叶提取废液中制备莽草酸的方法 |
CN109369373B (zh) * | 2018-12-18 | 2021-01-08 | 长江师范学院 | 一种从银杏叶提取物层析废液中制备莽草酸提取物的方法 |
CN109721487B (zh) * | 2019-01-15 | 2021-11-30 | 浙江海正药业股份有限公司 | 一种利用连续离子交换技术高效纯化莽草酸的工艺 |
CN112094184B (zh) * | 2019-06-17 | 2021-07-13 | 江苏得乐康生物科技有限公司 | 一种从银杏叶提取物层析废水中提取莽草酸的方法 |
CN110835297A (zh) * | 2019-10-29 | 2020-02-25 | 贵州贝因特生物技术有限公司 | 一种从银杏叶提取物生产废液中提取多种活性物质的方法 |
CN111087296A (zh) * | 2019-12-20 | 2020-05-01 | 东莞市东阳光生物合成药有限公司 | 提取莽草酸的方法和莽草酸提取物 |
CN111072468A (zh) * | 2019-12-25 | 2020-04-28 | 东莞市东阳光生物合成药有限公司 | 提取莽草酸的方法和莽草酸提取物 |
CN111056941B (zh) * | 2019-12-26 | 2022-07-05 | 浙江康恩贝制药股份有限公司 | 一种利用银杏叶提取物层析废液制备高纯度莽草酸的方法 |
CN113511967B (zh) * | 2020-04-10 | 2024-03-22 | 江苏得乐康生物科技有限公司 | 一种从银杏叶提取物层析废水中提取奎宁酸的方法 |
CN112250564B (zh) * | 2020-09-11 | 2023-10-20 | 宜昌东阳光生化制药有限公司 | 一种发酵液中提取莽草酸的方法 |
CN114436816B (zh) * | 2021-12-25 | 2024-05-28 | 新疆阜丰生物科技有限公司 | 一种离子交换技术高效提取莽草酸的方法 |
CN115006435B (zh) * | 2022-06-21 | 2023-11-28 | 浙江康恩贝制药股份有限公司 | 一种银杏叶有机酸提取物及其制备方法和用途 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1931823A (zh) * | 2006-09-29 | 2007-03-21 | 广西万山香料有限责任公司 | 从八角中提取分离莽草酸的方法 |
CN102584571A (zh) * | 2011-11-21 | 2012-07-18 | 河南孟成生物药业股份有限公司 | 一种发酵液中莽草酸的提取工艺 |
-
2016
- 2016-05-09 CN CN201610300286.4A patent/CN107353201B/zh active Active
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1931823A (zh) * | 2006-09-29 | 2007-03-21 | 广西万山香料有限责任公司 | 从八角中提取分离莽草酸的方法 |
CN102584571A (zh) * | 2011-11-21 | 2012-07-18 | 河南孟成生物药业股份有限公司 | 一种发酵液中莽草酸的提取工艺 |
Non-Patent Citations (2)
Title |
---|
Extraction and isolation of shikimic acid from Ginkgo biloba leaves utilizing an ionic liquid that dissolves cellulose;Toyonobu Usuki,et al.,;《Chem.Comm.》;20110825;第47卷;第10560-10562页 * |
莽草酸新资源及其分离纯化方法的研究;王祖磊;《中国优秀硕士学位论文全文数据库 农业科技辑》;20101115(第11期);论文的中文摘要 * |
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