CN107266193B - Aquatic weed carrier, its preparation method and special agar solution - Google Patents

Aquatic weed carrier, its preparation method and special agar solution Download PDF

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Publication number
CN107266193B
CN107266193B CN201710569585.2A CN201710569585A CN107266193B CN 107266193 B CN107266193 B CN 107266193B CN 201710569585 A CN201710569585 A CN 201710569585A CN 107266193 B CN107266193 B CN 107266193B
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aquatic
agar
carrier
final concentration
aquatic weed
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CN107266193A (en
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涂子璐
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B7/00Fertilisers based essentially on alkali or ammonium orthophosphates
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00Fertilisers characterised by their form
    • C05G5/20Liquid fertilisers

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Catching Or Destruction (AREA)
  • Fertilizers (AREA)

Abstract

The invention provides a waterweed carrier, a preparation method thereof and a special agar solution, relating to the technical field of ecological breeding. The agar solution provided by the invention comprises agar, a water plant liquid fertilizer and cytokinin. In addition, the invention also provides a waterweed carrier which is prepared by utilizing the agar solution and is suitable for the growth of waterweeds, and a preparation method of the waterweed carrier. The method for manufacturing the aquatic weed carrier provided by the invention has wide application range and is suitable for various aquatic weeds and fixed carriers. Moreover, the aquatic weed carrier provided by the invention contains short-acting and long-acting nutrient substances required by the growth of aquatic weeds, provides a stable and suitable environment for the growth of aquatic weeds, and a consumer only needs to simply put the aquatic weed carrier into an aquarium when planting the aquatic weed carrier, so that the fussy planting steps are simplified, the damage to plants is reduced, the survival rate of the aquatic weeds is improved, and the aquatic weed carrier has the characteristics of attractiveness and easiness in transportation.

Description

Aquatic weed carrier, its preparation method and special agar solution
Technical Field
The invention relates to the technical field of ecological breeding, in particular to a waterweed carrier, a manufacturing method thereof and a special agar solution.
Background
The existing aquatic weed production mode is to wrap the aquatic leaves or the aquatic leaves of aquatic weeds with asbestos or aquatic weed mud, and then put the aquatic leaves or the aquatic leaves into a special aquatic weed cup for sale. This mode of production has a number of disadvantages. For example, after the consumer purchases the aquatic weed, the aquatic weed mud or the aquatic weed cup needs to be removed, the aquatic weed, especially the aquatic weed root, which is very important for the growth of the aquatic weed, can be seriously damaged, and the damage of the aquatic weed root can directly cause the death of the aquatic weed. In addition, when the aquatic weeds are transplanted into the aquarium of the consumer, the aquatic weeds are not suitable for a new environment and die due to large environmental change difference and poor adaptability of the aquatic weeds. In addition, after asbestos, float grass or float grass is removed, nutrients are not provided, and float grass can die. In addition, the product of the production mode needs the consumers to have rich processing and planting experiences and perfectly control the planting conditions, density and other factors of the aquatic weeds, which also increases the difficulty for the planting of the consumers. If the production process is infected with pathogenic bacteria or parasites, the consumer's own aquarium is also directly affected. In addition, the method also has the disadvantages of inconvenient transportation, export and the like.
In addition, for epiphytic aquatic weeds, another mode of planting and selling by using carriers such as stainless steel mesh sheets, wood, stones and the like is provided. For example, Moss is stirred and broken, then spread on a stainless steel mesh, and then bound and fixed by fine fish wire until it grows. The production mode of using wood or stone is to bind and fix the epiphytic aquatic weeds on the stone wood by using fish wire or cotton thread, maintain the aquatic weeds in a greenhouse under a long-term spraying high-humidity environment, enable the aquatic weeds to grow leaves adapting to the environment and roots attached to a carrier, and then take the aquatic weeds to market for sale. However, the product also has the problems that the treatment of consumers is complicated, the aquatic weeds are not easy to use in a new environment, and the like. In addition, the carrier can not provide nutrient substances for the aquatic weeds at all, and is not beneficial to the growth of the aquatic weeds. Moreover, the method has large limitation and can only be applied to epiphytic aquatic weeds.
Therefore, the invention is especially provided for facilitating the planting of consumers, accelerating the aquatic weed to adapt to new environment and improving the survival rate of the aquatic weed.
Disclosure of Invention
The invention aims to provide an agar solution, a method for preparing a aquatic weed carrier, and a third aquatic weed carrier prepared by the method, so as to solve the problems of complicated planting operation and low survival rate of aquatic weed products in the prior art.
The present invention provides an agar solution comprising: agar with the final concentration of 12.5-21 g/L, a water plant liquid fertilizer with the final concentration of 4.0-5.5% (v/v), and cytokinin with the final concentration of 0.0045-0.0055% (v/v); the agar solution is used to provide nutrients to the aquatic weeds.
Further, the agar solution further comprises: biochemical fulvic acid potassium with the final concentration of 0.0405% -0.0495% (v/v), and/or aolvgao high potassium formula slow release fertilizer with the final concentration of 9-11 g/L.
In addition, the invention also provides a preparation method of the aquatic weed carrier, which comprises the following steps: heating and dissolving the agar solution, attaching the agar solution to a fixed carrier to form an agar jelly, immersing the aquatic weed in the agar jelly, and cooling and solidifying to form the aquatic weed carrier.
Further, the aquatic weeds are epiphytic aquatic weeds;
the agar solution is: agar with the final concentration of 17-21 g/L, a water plant liquid fertilizer with the final concentration of 4.0-5.0% (v/v), and cytokinin with the final concentration of 0.0045% -0.0055% (v/v); preferably, the agar solution is: agar at a final concentration of 19g/L, a liquid manure of aquatic weeds at a final concentration of 4.5% (v/v), and cytokinin at a final concentration of 0.005% (v/v);
the manufacturing method comprises the following steps: heating and dissolving the agar solution, and attaching the agar solution to a fixed carrier to form an agar jelly; and directly covering the epiphytic aquatic weeds on the agar jelly, extruding the epiphytic aquatic weeds to be close to the fixed carrier, and cooling and solidifying to form the aquatic weed carrier.
Further, the aquatic weeds are foreground aquatic weeds;
the agar solution is: agar with the final concentration of 16-20 g/L, a water-grass liquid fertilizer with the final concentration of 4.5-5.5% (v/v), cytokinin with the final concentration of 0.0045% -0.0055% (v/v) and biochemical fulvic acid potassium with the final concentration of 0.0405% -0.0495% (v/v); preferably, the agar solution is: agar with the final concentration of 18g/L, aquatic weed liquid fertilizer with the final concentration of 5% (v/v), cytokinin with the final concentration of 0.005% (v/v) and biochemical fulvic acid potassium with the final concentration of 0.045% (v/v);
the manufacturing method comprises the following steps: heating and dissolving the agar solution, and attaching the agar solution to a fixed carrier to form an agar jelly; and directly covering the foreground aquatic weeds on the agar jelly, extruding the foreground aquatic weeds to be close to the fixed carrier, and cooling and solidifying to form the aquatic weed carrier.
Further, the aquatic weeds are tuber aquatic weeds;
the agar solution is: agar with the final concentration of 14.5-17.5 g/L, a water-grass liquid fertilizer with the final concentration of 4.5-5.5% (v/v), cytokinin with the final concentration of 0.0045-0.0055% (v/v), biochemical fulvic acid potassium with the final concentration of 0.0405-0.0495% (v/v), and an ultramarine high-potassium formula slow release fertilizer with the final concentration of 9-11 g/L; preferably, the agar solution is: agar with the final concentration of 16g/L, a waterweed liquid fertilizer with the final concentration of 5% (v/v), cytokinin with the final concentration of 0.005% (v/v), biochemical fulvic acid potassium with the final concentration of 0.045% (v/v) and an ultramarine high-potassium formula slow release fertilizer with the final concentration of 10 g/L;
the manufacturing method comprises the following steps: heating and dissolving the prepared agar solution, and filling the agar solution on a fixed carrier to obtain an agar jelly attached to the fixed carrier; and removing all leaves of the tuber type aquatic weeds, only keeping 1-2 cm leaf stalks, and immersing tubers of the tuber type aquatic weeds in the agar jelly to ensure that growing points of new leaves of the tuber type aquatic weeds are exposed out of the agar jelly.
Further, the aquatic weeds are water weeds with stems;
the agar solution is: agar with the final concentration of 12.5-15.5 g/L, a water-grass liquid fertilizer with the final concentration of 4.5-5.5% (v/v), cytokinin with the final concentration of 0.0045-0.0055% (v/v), biochemical fulvic acid potassium with the final concentration of 0.0405-0.0495% (v/v), and an ultramarine high-potassium formula slow release fertilizer with the final concentration of 9-11 g/L; preferably, the agar solution is: agar with the final concentration of 14g/L, a waterweed liquid fertilizer with the final concentration of 5% (v/v), cytokinin with the final concentration of 0.005% (v/v), biochemical fulvic acid potassium with the final concentration of 0.045% (v/v) and an ultramarine high-potassium formula slow release fertilizer with the final concentration of 10 g/L;
the manufacturing method comprises the following steps: heating and dissolving the prepared agar solution, and filling the agar solution on a fixed carrier to obtain an agar jelly attached to the fixed carrier; removing the top, the bottom and original leaves of the stemmed aquatic weeds, then cutting the stemmed aquatic weeds into a plurality of stemmed aquatic weeds sections with the lengths of 6-8 cm, putting the stemmed aquatic weeds on the agar jelly, and naturally immersing the stemmed aquatic weeds in the agar jelly.
In addition, the invention also provides the aquatic weed carrier manufactured by the aquatic weed carrier manufacturing method.
Further, after the aquatic plant carrier is manufactured, the method further comprises a cultivation process, wherein the cultivation process comprises the following steps:
(a) carrying out first-stage culture on the aquatic weed carrier until the aquatic weed grows out a new bud;
the culture conditions of the first stage culture include: the air humidity is 85-100%, the temperature is 20-35 ℃, the illumination intensity is 10000-30000 LX, and the illumination time is more than 8h per day;
(b) performing second-stage culture on the aquatic weed carrier;
the culture conditions of the second stage culture include: the air humidity is 80-90%, the temperature is 25-35 ℃, the illumination intensity is 20000-50000 LX, and the illumination time is more than 10h per day;
spraying water twice a day, and spraying 0.5% potassium dihydrogen phosphate solution once every three days after the water is sprayed;
(c) carrying out third-stage culture on the aquatic weed carrier;
the culture conditions of the third stage culture include: the air humidity is 80-90%, the temperature is 25-35 ℃, the illumination intensity is 20000-50000 LX, and the illumination time is more than 10h per day;
managing the aquatic weeds, wherein the rules of management comprise: removing the top end of the ludwigia octovalvis exuberant in leaf growth, and spraying cytokinin on the ludwigia octovalvis exuberant in leaf growth;
(d) carrying out fourth-stage culture on the aquatic weed carrier;
the culture conditions of the fourth stage culture include: the air humidity is 50% -70%, the illumination intensity is 10000-30000 LX, and the illumination time is 6-8 h every day;
watering every other day.
The invention prepares the aquatic weed carrier suitable for the growth of aquatic weeds by optimizing a proper agar solution formula and creatively utilizing the characteristics of agar. The aquatic plant carrier provided by the invention contains nutrient substances required by the growth of aquatic plants, provides a stable and suitable environment for the growth of aquatic plants, and a consumer only needs to simply put the aquatic plant carrier into an aquarium when planting the aquatic plant carrier, so that the fussy planting steps are simplified, the damage to plants is reduced, and the survival rate of the aquatic plants is improved.
Detailed Description
The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments, and it should be understood that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The aquatic weed liquid fertilizer involved in the invention is as follows:
21g/L potassium nitrate, 5g/L urea, 30g/L potassium dihydrogen phosphate, 41g/L potassium sulfate, 8.053g/L ferrous sulfate heptahydrate, 15.5g/L magnesium sulfate, 1.66g/L boric acid, 1.17g/L manganese sulfate, 0.05g/L copper sulfate pentahydrate, 0.255g/L zinc sulfate heptahydrate, 15g/L disodium diamine tetraacetate, 2 g/L-ascorbic acid and 20g/L potassium fulvate.
The ultramarine high-potassium formula slow release fertilizer related in the invention is a conventional 328S type precision ultramarine fertilizer sold in the market.
The agar jelly, agar solid, referred to in the present invention, is explained as follows: after the solution containing the agar is heated and dissolved, the solution can be gradually solidified into a solid in the cooling process, and the solution is called as the agar solid after being completely solidified into the solid; before completely solidifying, it will take on a jelly-like semi-solid state called agar jelly.
The fixing carrier of the invention refers to all objects which can be adhered with agar jelly on the surface, and all objects which can play a bearing role can be used as the fixing carrier, including but not limited to stone, wood, pudding cup and other objects. In addition, it is essential that some porous objects can also be used as fixing carriers to allow the agar gel to adhere to the pores, including but not limited to porous stone.
The invention relates to a aquatic weed carrier, which is a product formed by wrapping an immobilized carrier and aquatic weed by agar solid.
The epiphytic aquatic weeds related to the invention can be, for example, but are not limited to: moss and Cassia nomame.
The foreground aquatic weeds related to the invention can be, for example, but are not limited to: mini short pearl, lawn pennywort herb, mozzie grass and groveling ground pearl.
The tuber aquatic weeds related to the invention can be, but are not limited to: pepper grass, water lily and water banyan aquatic grass.
The stemmed aquatic weeds related to the present invention may be, for example, but not limited to: red green palace, small red berry, and Newton grass.
When evaluating the growth condition of the aquatic weeds, the invention simulates the condition of culturing the aquatic weeds by consumers, which is called as a common culture environment in the application and is specifically represented as follows: placing the fish in an aquarium, wherein the illumination intensity is 20000LX, the daily illumination time is 8h, and the temperature is 28 ℃.
When the survival rate of the aquatic weeds is evaluated, the death standard is evaluated by professionals and mainly shows that leaves are wilted and plants stop growing.
First, the present invention provides an agar solution comprising: agar with the final concentration of 12.5-21 g/L, a water plant liquid fertilizer with the final concentration of 4-5.5% (v/v), and cytokinin with the final concentration of 0.0045-0.0055% (v/v); the agar solution is used to provide nutrients to aquatic weeds.
In addition, the invention also provides a preparation method of the aquatic weed carrier, which comprises the following steps: heating and dissolving the prepared agar solution, attaching the agar solution to a fixed carrier to form an agar jelly attached to the fixed carrier, immersing the aquatic weed in the agar jelly, and cooling and solidifying to form the aquatic weed carrier.
Wherein the agar jelly is attached to the immobilization carrier by a variety of methods including, but not limited to, spreading, pouring, soaking, and the like.
In addition, the invention also provides the aquatic weed carrier prepared by the method for preparing the aquatic weed carrier.
The invention creatively utilizes the characteristics of the agar to prepare the aquatic weed carrier. On one hand, nutrient substances required by growth of the aquatic weeds are uniformly distributed in the agar solid, so that the normal growth of the aquatic weeds is ensured; on the other hand, the agar solid wraps the aquatic weeds, so that the damage to aquatic weeds, especially the roots of the aquatic weeds, in the processes of transportation, transfer and the like is reduced; on the other hand, when the aquatic plant carrier is cultured, agar solid does not need to be removed, so that the damage to aquatic plant plants, especially to the root system of the aquatic plant, in the culture process is reduced; on the other hand, the aquatic weed carrier can purposefully control the density of the aquatic weeds in the manufacturing process, and the phenomena of over-dense planting or over-sparse planting and the like of the aquatic weeds caused by insufficient experience of consumers are avoided; on the other hand, the characteristics of the agar are utilized, the aquatic weeds can be conveniently adhered to the fixed carrier, and the arrangement of the aquarium by a consumer is facilitated; on the other hand, the agar has the characteristic of liquid before being completely solidified and can be attached to an irregular object or a porous structure fixing carrier, so that the selection of the fixing carrier is increased, and more possibilities are provided for the aquarium scene; on the other hand, the agar has good stability, and the aquatic weed carrier can be preserved in the aquarium for a long time; on the other hand, the agar is nontoxic and harmless, and has no adverse effect on the growth of the aquatic weeds; on the other hand, the preparation of the agar solution can be aseptic and pollution-free, is convenient to transport and store and is convenient for the export of aquatic weed carriers. After purchasing the aquatic weed carrier provided by the invention, consumers can achieve the purposes of cultivation and scenery arrangement by simply placing the aquatic weed carrier in own aquarium, and the aquatic weed carrier is simple and convenient and has high survival rate.
In addition, the invention also provides a targeted agar solution aiming at different types of aquatic weeds.
For example, when the aquatic weed is epiphytic aquatic weed, the agar solution (hereinafter referred to as "epiphytic aquatic weed agar solution") is: agar with the final concentration of 17-21 g/L, a water plant liquid fertilizer with the final concentration of 4.0-5.0% (v/v), and cytokinin with the final concentration of 0.0045% -0.0055% (v/v); preferably, the agar solution is: agar at a final concentration of 19g/L, a liquid manure of aquatic weeds at a final concentration of 4.5% (v/v), and cytokinin at a final concentration of 0.005% (v/v).
In addition, the present invention provides a method for producing a aquatic weed carrier in which aquatic weeds are epiphytic aquatic weeds (hereinafter, referred to as "epiphytic aquatic weed carrier"), comprising: heating and dissolving the epiphytic aquatic weed agar solution, and attaching the solution to a fixed carrier to form an agar jelly; directly covering the epiphytic aquatic weeds on the agar jelly, pressing the epiphytic aquatic weeds to be close to the fixed carrier, and cooling and solidifying to form the epiphytic aquatic weeds carrier.
When the epiphytic aquatic weeds are directly covered on the agar jelly, the fixing carrier has an upward surface and a downward surface in the actual use or placement process, preferably, the epiphytic aquatic weeds are immersed in the agar jelly on the upward surface of the fixing carrier, so that the epiphytic aquatic weeds can be ensured to grow normally, more preferably, the area of the epiphytic aquatic weeds is 75% -85% of the area of the upward surface of the fixing carrier, and thus, the growth density and the ornamental value of the epiphytic aquatic weeds can be coordinated; when the epiphytic aquatic weeds are pressed to be close to the fixed carrier, the distance between the epiphytic aquatic weeds and the surface of the fixed carrier close to the epiphytic aquatic weeds is preferably less than 2 mm; in the aquatic weed carrier of the epiphytic aquatic weed, the epiphytic aquatic weed can be completely immersed in the agar solid or partially immersed in the agar solid.
In addition, the invention also provides the epiphytic aquatic weed carrier prepared by the preparation method.
The epiphytic aquatic weed carrier provided by the invention has the advantages shared by aquatic weed carriers and also has unique characteristics. On one hand, the agar solution used in the epiphytic aquatic weed carrier is the epiphytic aquatic weed agar solution which has pertinence to the growth of the epiphytic aquatic weeds; on the other hand, a method for manufacturing the specific epiphytic aquatic weed carrier is selected according to the general characteristics of the epiphytic aquatic weeds.
For example, when the aquatic weed is a foreground aquatic weed, the agar solution (hereinafter referred to as "foreground aquatic weed agar solution") is: agar with the final concentration of 16-20 g/L, a water plant liquid fertilizer with the final concentration of 4.5-5.5% (v/v), cytokinin with the final concentration of 0.0045-0.0055% (v/v) and biochemical fulvic acid potassium with the final concentration of 0.00405-0.00495% (v/v); preferably, the agar solution is: agar at a final concentration of 18g/L, a liquid manure of waterweeds at a final concentration of 5% (v/v), cytokinin at a final concentration of 0.005% (v/v), and biochemical fulvic acid potassium at a final concentration of 0.0045% (v/v).
The water plants with the prospect have the characteristics of short plants and short and shallow root systems, and have low requirements on nutrients. Cytokinin and biochemical fulvic acid potassium are added on the basis of agar and a waterweed liquid fertilizer, so that the growth and survival of the foreground waterweed are better promoted. The cytokinin is used as a short-acting fertilizer, can provide nutrition required by the aquatic weeds in a short period, and can promote the growth of the root systems of the aquatic weeds; the biochemical potassium fulvate is used as a long-acting fertilizer, and can provide long-acting and long-lasting nutrition for the prospect aquatic weeds.
In addition, the invention also provides a method for manufacturing a aquatic weed carrier (hereinafter referred to as a 'foreground aquatic weed carrier') taking aquatic weeds as foreground aquatic weeds, which comprises the following steps: heating and dissolving the foreground waterweed agar solution, and attaching the foreground waterweed agar solution to a fixed carrier to form an agar jelly; and directly covering the foreground aquatic weeds on the agar jelly, pressing the foreground aquatic weeds to be close to the fixed carrier, and cooling and solidifying to form the foreground aquatic weeds carrier.
When the foreground aquatic weeds are directly covered on the agar jelly, the fixing carrier has an upward surface and a downward surface in the actual use or placing process, and preferably, the foreground aquatic weeds are immersed in the agar jelly on the upward surface of the fixing carrier, so that the aquatic weeds can be ensured to grow normally; more preferably, the area of the foreground aquatic weeds is 75-85% of the area of the upward surface of the fixed carrier, so that the growth density and the ornamental value of the aquatic weeds can be coordinated; when the foreground aquatic weeds are pressed to be close to the fixed carrier, the distance between the foreground aquatic weeds and the surface of the fixed carrier close to the foreground aquatic weeds is preferably less than 2 mm; in the aquatic weed carrier of the foreground aquatic weed, the foreground aquatic weed can be completely immersed in the agar solid or partially immersed in the agar solid.
In addition, the invention also provides the foreground aquatic weed carrier prepared by the preparation method.
The foreground aquatic weed carrier provided by the invention has the advantages of the aquatic weed carrier, and also has unique characteristics. On one hand, the agar solution used in the foreground aquatic weed carrier is the foreground aquatic weed agar solution, wherein the foreground aquatic weed agar solution not only comprises a short-acting fertilizer, but also comprises a long-acting fertilizer, and long-acting and long-lasting nutrients are provided for the growth of the foreground aquatic weeds; on the other hand, a method for manufacturing the targeted foreground aquatic weed carrier is selected according to the general characteristics of the foreground aquatic weeds.
For example, when the aquatic weed is a tuber aquatic weed, the agar solution (hereinafter referred to as "tuber aquatic weed agar solution") is: agar with the final concentration of 14.5-17.5 g/L, a water-grass liquid fertilizer with the final concentration of 4.5-5.5% (v/v), cytokinin with the final concentration of 0.0045-0.0055% (v/v), biochemical fulvic acid potassium with the final concentration of 0.0405-0.0495% (v/v), and an ultramarine high-potassium formula slow release fertilizer with the final concentration of 9-11 g/L; preferably, the agar solution is: agar with the final concentration of 16g/L, a waterweed liquid fertilizer with the final concentration of 5% (v/v), cytokinin with the final concentration of 0.005% (v/v), biochemical fulvic acid potassium with the final concentration of 0.045% (v/v), and an ultramarine high-potassium formula slow-release fertilizer with the final concentration of 10 g/L.
In addition, the invention also provides a method for preparing a aquatic weed carrier (hereinafter referred to as 'tuber aquatic weed carrier') with aquatic weeds being tuber aquatic weeds, which comprises the following steps: heating and dissolving prepared tuber pasture water agar solution, and attaching to a fixed carrier to form an agar jelly attached to the fixed carrier; removing all original leaves of the tuber waterweed, only keeping 1-2 cm leafstalks, immersing tubers of the tuber waterweed in the agar jelly, and ensuring that growing points of new leaves of the tuber waterweed are exposed out of the agar jelly.
Wherein, when the tuber of the tuber aquatic weed is immersed in the agar jelly, preferably, the side with the largest surface area of the tuber aquatic weed is immersed in the agar jelly and is flatly attached to the surface of the carrier.
In addition, the invention also provides a tuber aquatic plant carrier prepared by the preparation method.
The tuber aquatic weed carrier provided by the invention has the advantages shared by aquatic weed carriers, and also has unique characteristics. On one hand, the agar solution used in the tuber aquatic weed carrier is tuber aquatic weed agar solution, and has pertinence to the growth of tuber aquatic weeds; on the other hand, according to the characteristics of the tuber aquatic weeds, a targeted tuber aquatic weed carrier manufacturing method is selected, and the leaves are removed, so that important tuber parts are reserved.
For example, when the aquatic weeds are stemmed aquatic weeds, the agar solution (hereinafter referred to as "stemmed aquatic weeds agar solution") is: agar with the final concentration of 12.5-15.5 g/L, a water-grass liquid fertilizer with the final concentration of 4.5-5.5% (v/v), cytokinin with the final concentration of 0.0045-0.0055% (v/v), biochemical fulvic acid potassium with the final concentration of 0.0405-0.0495% (v/v), and an ultramarine high-potassium formula slow release fertilizer with the final concentration of 9-11 g/L; preferably, the agar solution is: agar with the final concentration of 14g/L, a waterweed liquid fertilizer with the final concentration of 5% (v/v), cytokinin with the final concentration of 0.005% (v/v), biochemical fulvic acid potassium with the final concentration of 0.045% (v/v), and an ultramarine high-potassium formula slow-release fertilizer with the final concentration of 10 g/L.
In addition, the present invention provides a method for preparing a vector for aquatic weeds having stems (hereinafter, referred to as "vector for aquatic weeds having stems"), comprising: heating and dissolving the prepared stemmed waterweed agar solution, and attaching the solution to a fixed carrier to obtain an agar jelly attached to the fixed carrier; removing the top, bottom and original leaves of the stemmed aquatic weed, cutting into several stemmed aquatic weed segments, putting the stemmed aquatic weed segments into agar jelly, naturally soaking the stemmed aquatic weed segments into the agar jelly, and cooling to obtain the stemmed aquatic weed carrier.
Wherein, when the top end of the stemmed aquatic weed is removed, preferably, the top end of the stemmed aquatic weed is removed by 5cm below the top end of the upper leaf; when the bottom of the stemmed aquatic weed is removed, preferably, the bottom of the white light-colored rhizome is removed by more than 5 cm; when the water plants with stems are cut into a plurality of water plant sections with stems, preferably, the water plants with stems are cut into a plurality of water plant sections with stems and lengths of 6-8 cm; when the stemmed aquatic weed pieces are placed in the agar jelly, it is preferable that the stemmed aquatic weed pieces cover 50% of the surface of the agar jelly.
In addition, the invention also provides the stemmed aquatic weed carrier prepared by the preparation method.
The stemmed aquatic weed carrier provided by the invention has the advantages shared by aquatic weed carriers, and also has unique characteristics. On one hand, the agar solution used in the stemmed aquatic weed carrier is a stemmed aquatic weed agar solution which has pertinence to the growth of the stemmed aquatic weeds; on the other hand, according to the characteristics of the stemmed aquatic weeds, a method for manufacturing the stemmed aquatic weed carrier is selected, the leaves are removed, and the stems are reserved for proliferation.
In addition, the aquatic weed carrier also comprises a culture process after the aquatic weed carrier is manufactured, and according to the unique characteristics of aquatic weed carrier products, the culture environment is optimized, the culture process is carried out, the aquatic weeds can grow well, and finally, excellent aquatic weeds with high survival rate and strong quality are obtained.
In addition, the invention also provides preparation before shipment of the aquatic weed carrier, which comprises the following steps: (1) and (4) observing by naked eyes, and selecting the carriers with the surface of the carriers with the aquatic weeds growing vigorously and the aquatic weeds growing coverage area reaching more than 90%. (2) The bottom of the vector was examined for normal growth, with the normal vector having a large number of new white roots and no black necrotic roots. (3) Checking whether abnormal objects including snail, egg, worm and the like exist or not, and whether the abnormal objects do not exist is a qualified vector. Then the carrier is soaked in potassium permanganate with the concentration of 0.02 percent for 5 minutes for disinfection. (4) And (7) discharging.
To facilitate a better understanding of the present invention, reference will now be made in detail to the following specific examples.
Example 1-1 Epimedium solution
The embodiment provides a epiphytic aquatic weed agar solution, which comprises the following components in percentage by weight:
Figure 898528DEST_PATH_IMAGE001
the agar solution provided by the embodiment can provide nutrition for the epiphytic aquatic weeds.
Example 1-2 Epimedium solution
The embodiment provides a epiphytic aquatic weed agar solution, which comprises the following components in percentage by weight:
Figure 202470DEST_PATH_IMAGE002
examples 1-3 Epimedium solution
The embodiment provides a epiphytic aquatic weed agar solution, which comprises the following components in percentage by weight:
Figure 477593DEST_PATH_IMAGE003
examples 1-4 preparation of Ephedra aquatica Carriers
Compounding the epiphytic aquatic weed agar solution according to the formula, heating at high temperature for sterilization, and attaching the agar solution to a fixed carrier when the solution is cooled to about 40 ℃ to form an agar jelly attached to the fixed carrier.
Directly cutting the grass or grass seeds into a size of about 80% of the coverage area, directly covering the agar jelly, squeezing the aquatic weeds by fingers or other tools to be close to the carrier as much as possible, keeping the agar jelly (agar with the thickness of less than 2 mm) with less contact area with the aquatic weeds, and keeping the rest squeezed agar jelly and the agar jelly which is not covered with the aquatic weeds to allow the aquatic weeds to grow and cover naturally.
Concentration optimization of aquatic weed liquid fertilizer in epiphytic aquatic weed agar solution
In the case of Moss (Epimedium aquilinum) as an example, Epimedium aquilinum carriers were prepared from different agar solutions according to the methods of examples 1-4 to determine the optimum concentrations of the components in the agar solutions. In the optimization experiment, the concentrations of the agar and the cytokinin are fixed (wherein, the concentration of the agar is 19g/L, and the concentration of the cytokinin is 0.005% (v/v)), the concentration of the liquid manure of the aquatic weeds is changed, the aquatic weeds are cultured for 20 days in a common culture environment, the survival rate of the aquatic weeds is calculated, and the survival rate of the aquatic weeds is recorded as the following table.
Figure 121064DEST_PATH_IMAGE004
Note: represents a significant difference compared to the 2% group (P < 0.05);
# represents a significant difference compared to the 7% group (P < 0.05).
According to the table, the survival rate of the epiphytic aquatic weeds can reach over 67% when the concentration of the aquatic weed liquid fertilizer is 4-5% (v/v), and particularly reaches 76% when the concentration of the aquatic weed liquid fertilizer is 4.5% (v/v). And when the concentration of the aquatic weed liquid fertilizer is too low (2%) or too high (7%), the growth of the epiphytic aquatic weed is not facilitated.
Concentration optimization of cytokinin in epiphytic aquatic weed agar solution
In the case of Moss (Epimedium aquilinum) as an example, Epimedium aquilinum carriers were prepared from different agar solutions according to the methods of examples 1-4 to determine the optimum concentrations of the components in the agar solutions. In the optimization experiment, the concentrations of the agar and the aquatic weed liquid fertilizer are fixed (wherein, the concentration of the agar is 19g/L, and the concentration of the aquatic weed liquid fertilizer is 4.5% (v/v)), the concentration of the cytokinin is changed, the aquatic weed is cultured for 20 days in a common culture environment, the survival rate of the aquatic weed is calculated, and the survival rate record of the aquatic weed is as follows.
Figure 518548DEST_PATH_IMAGE005
Note: represents a significant difference compared to the 0.001% group (P < 0.05);
# represents a significant difference compared to the 0.02% group (P < 0.05).
According to the table, the survival rate of the epiphytic aquatic weed can reach more than 68% when the concentration of the cytokinin is 0.0045% -0.0055% (v/v), and particularly reaches 76% when the concentration is 0.005% ((v/v)). When the concentration of cytokinin is too low (0.001%) or too high (0.02%), the growth of epiphytic aquatic weeds is not facilitated.
Example 2-1 prospect Water grass agar solution
The embodiment provides a foreground aquatic weed agar solution, which comprises the following components in percentage by weight:
Figure 309786DEST_PATH_IMAGE006
the agar formula provided by the embodiment is used for cultivating the foreground aquatic weeds.
Example 2-2 prospect Water weed agar solution
The embodiment provides a foreground aquatic weed agar solution, which comprises the following components in percentage by weight:
Figure 388601DEST_PATH_IMAGE007
example 2-3 prospect Water grass agar solution
The embodiment provides a foreground aquatic weed agar solution, which comprises the following components in percentage by weight:
Figure 972332DEST_PATH_IMAGE008
EXAMPLE 2-4 preparation of the Formica Fusca Carrier
Preparing the foreground aquatic weed agar solution according to a formula, heating at high temperature for sterilization, and attaching the agar solution to a fixed carrier when the temperature is cooled to about 40 ℃ to form an agar jelly attached to the fixed carrier.
Directly cutting the grass or grass seeds into a size of about 80% of the coverage area, directly covering the agar jelly, squeezing the aquatic weeds by fingers or other tools to be close to the carrier as much as possible, keeping the agar jelly (agar with the thickness of less than 2 mm) with less contact area with the aquatic weeds, and keeping the rest squeezed agar jelly and the agar jelly which is not covered with the aquatic weeds to allow the aquatic weeds to grow and cover naturally.
Concentration optimization of water plant liquid fertilizer in foreground water plant agar solution
Taking mini short pearl (prospect aquatic weed) as an example, preparing a prospect aquatic weed carrier by using different agar solutions according to the method of the example 2-4 so as to determine the optimal concentration of each component in the agar solution. In the optimization experiment, the concentrations of agar, cytokinin and biochemical potassium fulvate are fixed (wherein the concentration of the agar is 18g/L, the concentration of the cytokinin is 0.005% (v/v), and the concentration of the biochemical potassium fulvate is 0.045% (v/v)), the concentration of the liquid fertilizer of the waterweed is changed, the waterweed is cultured in a common culture environment for 40 days, the survival rate of the waterweed is calculated, and the survival rate of the waterweed is recorded as the following table.
Figure 806296DEST_PATH_IMAGE009
Note: represents a significant difference compared to the 2% group (P < 0.05);
# represents a significant difference compared to the 7% group (P < 0.05).
According to the table, the survival rate of the foreground aquatic weeds can reach more than 68% when the concentration of the aquatic weed liquid fertilizer is 4.5% -5.5% (v/v), and particularly reaches 82% when the concentration of the aquatic weed liquid fertilizer is 5% (v/v). And when the concentration of the aquatic weed liquid fertilizer is too low (2%) or too high (7%), the growth of the foreground aquatic weeds is not facilitated.
Concentration optimization of cytokinin in foreground waterweed agar solution
Taking mini short pearl (prospect aquatic weed) as an example, preparing a prospect aquatic weed carrier by using different agar solutions according to the method of the example 2-4 so as to determine the optimal concentration of each component in the agar solution. In the optimization experiment, the concentrations of the agar, the aquatic weed liquid fertilizer and the biochemical potassium fulvate are fixed (wherein the concentration of the agar is 18g/L, the concentration of the aquatic weed liquid fertilizer is 5% (v/v), and the concentration of the biochemical potassium fulvate is 0.045% (v/v)), the concentration of cytokinin is changed, the aquatic weed liquid fertilizer is cultured in a common culture environment for 40 days, the survival rate of the aquatic weed is calculated, and the survival rate of the aquatic weed is recorded as the following table.
Figure 756935DEST_PATH_IMAGE010
Note: represents a significant difference compared to the 0.001% group (P < 0.05);
# represents a significant difference compared to the 0.2% group (P < 0.05).
The table shows that the survival rate of the foreground aquatic weeds can reach more than 63% when the concentration of the cytokinin is 0.0045% -0.0055% (v/v), and particularly reaches 82% when the concentration of the cytokinin is 0.005% (v/v). When the concentration of cytokinin is too low (0.001%) or too high (0.02%), the growth of the foreground aquatic weeds is not facilitated.
Concentration optimization of biochemical fulvic acid potassium in foreground aquatic weed agar solution
Taking mini short pearl (prospect aquatic weed) as an example, preparing a prospect aquatic weed carrier by using different agar solutions according to the method of the example 2-4 so as to determine the optimal concentration of each component in the agar solution. In the optimization experiment, the concentrations of the agar, the aquatic weed liquid fertilizer and the cytokinin are fixed (wherein the concentration of the agar is 18g/L, the concentration of the aquatic weed liquid fertilizer is 5% (v/v), and the concentration of the cytokinin is 0.005% (v/v)), the concentration of the biochemical potassium fulvate is changed, the aquatic weed is cultured in a common culture environment for 40 days, the survival rate of the aquatic weed is calculated, and the survival rate of the aquatic weed is recorded as the following table.
Figure 701757DEST_PATH_IMAGE011
Note: represents a significant difference compared to the 0.001% group (P < 0.05);
# represents a significant difference compared to the 0.2% group (P < 0.05).
According to the table, the survival rate of the foreground aquatic weeds can reach over 74% when the concentration of the biochemical potassium fulvate is 0.0405% -0.0495% (v/v), and particularly reaches 82% when the concentration of the biochemical potassium fulvate is 0.045% (v/v). When the concentration of the biochemical potassium fulvate is too low (0%) or too high (0.2%), the growth of the foreground aquatic weeds is not facilitated.
EXAMPLE 3-1 Tuber pasture and Water agar solution
The embodiment provides a tuber aquatic weed agar solution, which comprises the following components in percentage by weight:
Figure 54241DEST_PATH_IMAGE012
the agar formulation provided in this example was used to cultivate tuber pasture.
Example 3-2 Tuber Water weed agar solution
The embodiment provides a tuber aquatic weed agar solution, which comprises the following components in percentage by weight:
Figure 59106DEST_PATH_IMAGE013
example 3-3 Tuber Water weed agar solution
The embodiment provides a tuber aquatic weed agar solution, which comprises the following components in percentage by weight:
Figure 497041DEST_PATH_IMAGE014
EXAMPLE 3-4 preparation of tuber waterweed vectors
The tuber aquatic weed agar solution is prepared according to the formula, and then is heated and sterilized at high temperature, when the tuber aquatic weed agar solution is cooled to about 40 ℃, the agar solution is attached to a fixed carrier to form an agar jelly attached to the fixed carrier.
Removing all original leaves of the tuber type aquatic weeds, only keeping about 1-2 cm of leafstalks, implanting the surface with the largest surface area of the tubers into the agar jelly, flatly pasting the surface of the carrier as much as possible, and exposing the growth points of the new leaves of the aquatic weeds to the agar.
Concentration optimization of water plant liquid fertilizer in tuber water plant agar solution
Using Pepper grass (tuber waterweed) as an example, tuber waterweed carriers were prepared according to the methods of examples 3-4 using different agar solutions to determine the optimal concentrations of the components in the agar solutions. In the optimization experiment, the concentrations of agar, cytokinin, biochemical potassium fulvate and ultramarine high potassium formula slow release fertilizer are fixed (wherein the concentration of agar is 16g/L, the concentration of cytokinin is 0.005% (v/v), the concentration of biochemical potassium fulvate is 0.045% (v/v), and the concentration of ultramarine high potassium formula slow release fertilizer is 10 g/L), the concentration of the liquid fertilizer of the waterweed is changed, the cultivation is carried out for 40 days in a common cultivation environment, the survival rate of the waterweed is calculated, and the survival rate record of the waterweed is as shown in the following table.
Figure 979975DEST_PATH_IMAGE015
Note: represents a significant difference compared to the 2% group (P < 0.05);
# represents a significant difference compared to the 7% group (P < 0.05).
According to the table, the survival rate of the tuber aquatic weeds can reach more than 63% when the concentration of the aquatic weed liquid fertilizer is 4.5% -5.5% (v/v), and particularly reaches 79% when the concentration of the aquatic weed liquid fertilizer is 5% (v/v). When the concentration of the liquid fertilizer for the aquatic weeds is too low (2%) or too high (7%), the growth of tuber aquatic weeds is not facilitated.
Concentration optimization of cytokinin in tuber water plant agar solution
Using Pepper grass (tuber waterweed) as an example, tuber waterweed carriers were prepared according to the methods of examples 3-4 using different agar solutions to determine the optimal concentrations of the components in the agar solutions. In the optimization experiment, the concentrations of agar, a waterweed liquid fertilizer, biochemical fulvic acid potassium and an ultramarine high-potassium formula slow release fertilizer are fixed (wherein the concentration of the agar is 16g/L, the concentration of the waterweed liquid fertilizer is 5% (v/v), the concentration of the biochemical fulvic acid potassium is 0.045% (v/v), and the concentration of the ultramarine high-potassium formula slow release fertilizer is 10 g/L), the concentration of cytokinin is changed, the cultivation is carried out for 40 days in a common cultivation environment, the survival rate of the waterweed is calculated, and the survival rate record of the waterweed is as shown in the following table.
Figure 452544DEST_PATH_IMAGE016
Note: represents a significant difference compared to the 0.001% group (P < 0.05);
# represents a significant difference compared to the 0.2% group (P < 0.05).
According to the table, the survival rate of the tuber aquatic weeds can reach more than 63% when the concentration of the cytokinin is 0.0045% -0.0055% (v/v), and particularly reaches 79% when the concentration of the cytokinin is 0.005% (v/v). When the concentration of cytokinin is too low (0.001%) or too high (0.02%), the growth of tuber aquatic weeds is not facilitated.
Concentration optimization of biochemical fulvic acid potassium in tuber waterweed agar solution
Using Pepper grass (tuber waterweed) as an example, tuber waterweed carriers were prepared according to the methods of examples 3-4 using different agar solutions to determine the optimal concentrations of the components in the agar solutions. In the optimization experiment, the concentrations of agar, a waterweed liquid fertilizer, cytokinin and an ultramarine high-potassium formula slow release fertilizer are fixed (wherein, the concentration of the agar is 16g/L, the concentration of the waterweed liquid fertilizer is 5% (v/v), the concentration of the cytokinin is 0.005% (v/v), and the concentration of the ultramarine high-potassium formula slow release fertilizer is 10 g/L), the concentration of biochemical fulvic acid potassium is changed, the cultivation is carried out for 40 days in a common cultivation environment, the survival rate of the waterweed is calculated, and the survival rate of the waterweed is recorded as the following table.
Figure 628311DEST_PATH_IMAGE017
Note: represents a significant difference compared to the 0% group (P < 0.05);
# represents a significant difference compared to the 0.2% group (P < 0.05).
According to the table, the survival rate of the tuber aquatic weeds can reach more than 63% when the concentration of the biochemical potassium fulvate is 0.0405% -0.0495% (v/v), and particularly reaches 79% when the concentration of the biochemical potassium fulvate is 0.045% (v/v). When the concentration of the biochemical potassium fulvate is too low (0%) or too high (0.2%), the tuber aquatic weeds are not beneficial to growth.
Concentration optimization of aolv high-potassium formula slow-release fertilizer in tuber waterweed agar solution
Using Pepper grass (tuber waterweed) as an example, tuber waterweed carriers were prepared according to the methods of examples 3-4 using different agar solutions to determine the optimal concentrations of the components in the agar solutions. In the optimization experiment, the concentrations of agar, the aquatic weed liquid fertilizer, the cytokinin and the biochemical potassium fulvate (wherein the concentration of the agar is 16g/L, the concentration of the aquatic weed liquid fertilizer is 5% (v/v), the concentration of the cytokinin is 0.005% (v/v) and the concentration of the biochemical potassium fulvate is 0.045% (v/v)) are fixed, the concentration of the aolv high-potassium formula slow-release fertilizer is changed, the cultivation is carried out for 40 days in a common cultivation environment, the survival rate of the aquatic weed is calculated, and the survival rate of the aquatic weed is recorded as the following table.
Figure 553541DEST_PATH_IMAGE018
Note: represents a significant difference compared to the 2g/L group (P < 0.05);
# represents a significant difference compared to the 20g/L group (P < 0.05).
According to the table, the survival rate of the tuber aquatic weeds can reach over 68% when the concentration of the aolv high-potassium formula slow release fertilizer is 9-11 g/L, and particularly reaches 79% when the concentration of the aolv high-potassium formula slow release fertilizer is 10 g/L. When the concentration of the AoLVHEKAI formula slow release fertilizer is too low (2 g/L) or too high (20 g/L), the growth of tuber aquatic weeds is not facilitated.
Example 4-1 agar solution for use in growing stemmed aquatic weeds
The embodiment provides a stemmed aquatic weed agar solution, which comprises the following components in percentage by weight:
Figure 777849DEST_PATH_IMAGE019
the agar formulation provided in this example was used to cultivate stemmed aquatic weeds.
Example 4-2 agar solution for cultivation of stemmed waterweed
The embodiment provides a stemmed aquatic weed agar solution, which comprises the following components in percentage by weight:
Figure 901663DEST_PATH_IMAGE020
example 4-3 agar solution for cultivation of stemmed waterweed
The embodiment provides a stemmed aquatic weed agar solution, which comprises the following components in percentage by weight:
Figure 186014DEST_PATH_IMAGE021
example 4-4 preparation of stemmed waterweed vectors
The preparation method comprises preparing herba Oenotherae Erythrosepalae agar solution according to formula, heating at high temperature for sterilization, cooling to about 40 deg.C, and attaching the agar solution to the fixed carrier to form agar jelly attached to the fixed carrier.
Removing the top end of fresh ornamental aquatic weed with stem and leaf (5 CM below the top end), removing the root with white light color (5 CM above the bottom), and removing all the original leaves with scissors. Averagely cutting the stemmed grass into a plurality of sections, keeping about 6-8 CM in each section, uniformly and alternately paving the sections on the formula agar jelly, covering about 50% of the surface area of the jelly, naturally covering the stems without extruding the stems, and keeping more agar jelly on the surface of the carrier as much as possible.
Concentration optimization of water plant liquid fertilizer in water plant agar solution with stems
Using red and green palace (having stem aquatic plants) as an example, the stem aquatic plant carriers were prepared according to the methods of examples 4-4 using different agar solutions to determine the optimum concentrations of the components in the agar solutions. In the optimization experiment, the concentrations of agar, cytokinin, biochemical potassium fulvate and ultramarine high-potassium formula slow release fertilizer are fixed (wherein the concentration of agar is 14g/L, the concentration of cytokinin is 0.005% (v/v), the concentration of biochemical potassium fulvate is 0.045% (v/v), and the concentration of ultramarine high-potassium formula slow release fertilizer is 10 g/L), the concentration of the liquid fertilizer of the waterweed is changed, the cultivation is carried out for 40 days in a common cultivation environment, the survival rate of the waterweed is calculated, and the survival rate record of the waterweed is as shown in the following table.
Figure 660857DEST_PATH_IMAGE022
Note: represents a significant difference compared to the 2% group (P < 0.05);
# represents a significant difference compared to the 7% group (P < 0.05).
According to the table, the survival rate of the water plants with stems can reach more than 69% when the concentration of the water plant liquid fertilizer is 4.5% -5.5% (v/v), and particularly reaches 85% when the concentration of the water plant liquid fertilizer is 5% (v/v). When the concentration of the aquatic weed liquid fertilizer is too low (2%) or too high (7%), the growth of the stem aquatic weeds is not facilitated.
Concentration optimization of cytokinin in stemmed waterweed agar solution
Using red and green palace (having stem aquatic plants) as an example, the stem aquatic plant carriers were prepared according to the methods of examples 4-4 using different agar solutions to determine the optimum concentrations of the components in the agar solutions. In the optimization experiment, the concentrations of agar, a waterweed liquid fertilizer, biochemical fulvic acid potassium and an ultramarine high-potassium formula slow release fertilizer are fixed (wherein the concentration of the agar is 14g/L, the concentration of the waterweed liquid fertilizer is 5% (v/v), the concentration of the biochemical fulvic acid potassium is 0.045% (v/v), and the concentration of the ultramarine high-potassium formula slow release fertilizer is 10 g/L), the concentration of cytokinin is changed, the cultivation is carried out for 40 days in a common cultivation environment, the survival rate of the waterweed is calculated, and the survival rate record of the waterweed is as shown in the following table.
Figure 688856DEST_PATH_IMAGE023
Note: represents a significant difference compared to the 0.001% group (P < 0.05);
# represents a significant difference compared to the 0.2% group (P < 0.05).
From the above table, it can be found that the survival rate of the water plants with stems can be more than 67% when the concentration of the cytokinin is 0.0045% -0.0055% (v/v), and particularly the survival rate of the water plants with stems reaches 85% when the concentration of the cytokinin is 0.005% (v/v). When the concentration of cytokinin is too low (0.001%) or too high (0.02%), the growth of the stemmed aquatic weeds is not favored.
Concentration optimization of biochemical fulvic acid potassium in stemmed waterweed agar solution
Using red and green palace (having stem aquatic plants) as an example, the stem aquatic plant carriers were prepared according to the methods of examples 4-4 using different agar solutions to determine the optimum concentrations of the components in the agar solutions. In the optimization experiment, the concentrations of agar, a waterweed liquid fertilizer, cytokinin and an ultramarine high-potassium formula slow release fertilizer are fixed (wherein the concentration of the agar is 14g/L, the concentration of the waterweed liquid fertilizer is 5% (v/v), the concentration of the cytokinin is 0.005% (v/v), and the concentration of the ultramarine high-potassium formula slow release fertilizer is 10 g/L), the concentration of biochemical fulvic acid potassium is changed, the cultivation is carried out for 40 days in a common cultivation environment, the survival rate of the waterweed is calculated, and the survival rate of the waterweed is recorded as the following table.
Figure 932756DEST_PATH_IMAGE024
Note: represents a significant difference compared to the 0% group (P < 0.05);
# represents a significant difference compared to the 0.2% group (P < 0.05).
The table shows that the survival rate of the stemmed aquatic weeds can be more than 79% when the concentration of the biochemical potassium fulvate is 0.0405% -0.0495% (v/v), and particularly reaches 85% when the concentration of the biochemical potassium fulvate is 0.045% (v/v). When the concentration of the biochemical potassium fulvate is too low (0%) or too high (0.2%), the growth of the stemmed aquatic weeds is not facilitated.
Concentration optimization of AoLVHIGH-POTASSIUM formulated slow release fertilizer in stemmed waterweed agar solution
Using red and green palace (having stem aquatic plants) as an example, the stem aquatic plant carriers were prepared according to the methods of examples 4-4 using different agar solutions to determine the optimum concentrations of the components in the agar solutions. In the optimization experiment, the concentrations of agar, the aquatic weed liquid fertilizer, the cytokinin and the biochemical potassium fulvate (wherein the concentration of the agar is 16g/L, the concentration of the aquatic weed liquid fertilizer is 5% (v/v), the concentration of the cytokinin is 0.005% (v/v) and the concentration of the biochemical potassium fulvate is 0.045% (v/v)) are fixed, the concentration of the aolv high-potassium formula slow-release fertilizer is changed, the cultivation is carried out for 40 days in a common cultivation environment, the survival rate of the aquatic weed is calculated, and the survival rate of the aquatic weed is recorded as the following table.
Figure 184746DEST_PATH_IMAGE025
Note: represents a significant difference compared to the 2g/L group (P < 0.05);
# represents a significant difference compared to the 20g/L group (P < 0.05).
According to the table, the survival rate of the aquatic weeds with stems can reach over 73% when the concentration of the AoLVHENGGASUI formula slow release fertilizer is 9-11 g/L, and particularly reaches 85% when the concentration of the aquatic weeds with stems is 10 g/L. When the concentration of the AoLVHEKAI formula slow release fertilizer is too low (2 g/L) or too high (20 g/L), the growth of the stemmed aquatic weeds is not facilitated.
Example 9 cultivation of aquatic weed carriers
After the aquatic weed carrier provided by the invention is manufactured, the aquatic weed carrier can also comprise a culture process, and specifically comprises the following steps:
a preparation stage: the float grass carrier is put into clean water for washing, and the attached impurities are removed gently. And (4) drying excessive water on the surface of the float grass carrier, and simultaneously ensuring that the float grass is fixed on the surface of the fixed carrier. Spraying 0.1% cytokinin solution on the surface of the aquatic weed.
The first stage is as follows: in the first week, the agar carrier is placed in a greenhouse, the humidity of the culture air is controlled within the range of 85% -100%, the temperature is controlled within 20-35 ℃, the illumination intensity is controlled within 10000-30000 LX, the illumination time is kept above 8 hours per day, and an LED plant lamp is additionally used for light supplement in cloudy days. No additional watering and fertilizer addition is required. And observing the growth condition of the agar carrier aquatic weeds from 7 days to 10 days, wherein the aquatic weeds grow new buds under normal conditions, and the aquatic weeds are inspected to see whether the aquatic weeds die and are necrotic without the new buds, so that defective products with problems are treated. The aquatic weeds survive but have no carriers of the new buds, and are continuously cultivated under the condition of the first week. The carrier with the sprout enters the next stage for cultivation.
And a second stage: on the 10 th day to the 25 th day, the temperature of the greenhouse is controlled within 25 ℃ to 35 ℃, the illumination intensity is controlled within 20000LX to 50000LX, the illumination time is kept above 10 hours per day, an LED plant lamp is additionally used for light supplement on a cloudy day, the air humidity is controlled within 80 percent to 90 percent, water is sprayed for 2 times per day, and water is sprayed for 2 minutes to 3 minutes in the morning and evening each day; after spraying water every three days, 0.5% potassium dihydrogen phosphate solution was sprayed. Checking whether the pathogenic bacteria occur every day, and spraying water-soluble pesticide in time for treatment; checking whether the pH value of the aquaculture water body is kept between 6.0 and 7.5 every day.
And a third stage: observing the growth condition of the carrier after 20-25 days, wherein the exuberant growth of the leaves belongs to a qualified product; the sparse part of the plant part needs to be removed at the top end (5 CM below the top end is cut off), and the sparse part is sprayed with a cytokinin solution and is continuously cultivated in a second-stage environment. The carriers which have been grown well and have uniform density enter the next stage for cultivation.
A fourth stage: and (3) controlling the air humidity of the carrier culture environment to be 50% -70% in 25-30 days, so that the water content of the plants is reduced to adapt to a drier common environment, the illumination intensity is controlled within 10000-30000 LX, the illumination time is reduced to 6-8 hours per day, and the growth of the aquatic weeds is controlled. Keeping the bottom of the carrier moist, watering for 1 time every other day, stopping fertilizing, spraying pesticide, and the like.
EXAMPLE 10 preparation of aquatic weed Carrier for shipment
Aquatic weed carriers were grown according to the method provided in example 9 and reached shipment standards within a normal week. After shipment inspection and preparation before shipment, the product can be sold to consumers. The shipment inspection method comprises the following steps of observing by naked eyes, selecting carriers with the growth of aquatic weeds on the surfaces of the carriers being vigorous and the growth coverage area of the aquatic weeds reaching more than 90%; checking whether the bottom of the carrier grows normally (the normal carrier has a large amount of white new roots and does not have black and necrotic roots); whether abnormal objects exist or not is checked, wherein the abnormal objects comprise snails, eggs, worms and the like (the abnormal objects do not exist is a qualified carrier). After the test, the carrier is soaked in potassium permanganate with the concentration of 0.02 percent for 5 minutes for disinfection. And (5) delivering the finished product after disinfection to obtain the aquatic weed carrier.
Effect example aquatic weed carrier transplanting survival rate evaluation
Aiming at different varieties of aquatic weeds, the survival rate of the aquatic weed carrier obtained by the agar solution, the aquatic weed carrier manufacturing method and the culture and shipment preparation process is evaluated, and the specific experimental contents and results are as follows.
Experimental group 1: the carrier of the epiphytic aquatic weed is obtained by taking Moss (epiphytic aquatic weed) as an example and adopting the methods of the examples 1-1, 1-4, 9 and 10.
Control group 1: the conventional waterweed mud is commercially available to wrap the moss (epiphytic waterweed) on the roots.
Experimental group 2: taking mini short pearl (prospect aquatic weed) as an example, the prospect aquatic weed carrier is obtained according to the methods of the examples 2-1, 2-4, 9 and 10.
Control group 2: the mini short pearl (prospect waterweed) at the root is wrapped by the conventional waterweed mud in the market.
Experimental group 3: tuber waterweed vectors obtained in the same manner as in examples 3-1, 3-4, 9 and 10, using Pepper waterweed (tuber waterweed) as an example.
Control group 3: the conventional waterweed mud is commercially available to wrap the roots of the peppers (tuber waterweeds).
Experimental group 4: using red and green palace (stemmed aquatic weeds) as an example, the stemmed aquatic weeds carriers obtained by the methods of examples 4-1, 4-4, 9 and 10 were obtained.
Control group 4: red and green palace (with stem aquatic plants) with roots wrapped by conventional aquatic plant mud is sold in the market.
50 products are respectively selected from each experimental group and each control group, and are cultured in a common culture environment to simulate the culture process of consumers. Directly putting the experimental group products into an aquarium in a common culture environment for culture; and (4) cleaning the aquatic plant mud wrapping the roots of the contrast group product, and then putting the aquatic plant mud into an aquarium in a common culture environment for culture. And after culturing for 60 days, recording the survival rate of the aquatic weeds in each group.
Figure 84569DEST_PATH_IMAGE026
As can be seen from the data in the table above, the aquatic weed carriers prepared by the agar solution provided by the invention are cultured and screened, and the obtained aquatic weed carriers can survive completely without special treatment after being transplanted into a common culture environment; in contrast, the survival rate of the commercial common aquatic weed products is only about 50%. Moreover, the growth conditions of the aquatic weeds of each experimental group and the control group are observed at any time during the culture period, and the experimental group aquatic weeds are developed in root systems and healthy in color and are light white; the roots of many aquatic weeds in the control group are dark and rotten.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.

Claims (10)

1. A method for manufacturing a float grass carrier, which is characterized in that,
the aquatic weeds are epiphytic aquatic weeds;
the manufacturing method comprises the following steps: heating and dissolving the agar solution, and attaching the agar solution to a fixed carrier to form an agar jelly; directly covering the epiphytic aquatic weeds on the agar jelly, pressing the epiphytic aquatic weeds to be close to the fixed carrier, and cooling and solidifying to form the aquatic weed carrier;
the agar solution is: agar with the final concentration of 17-21 g/L, a water plant liquid fertilizer with the final concentration of 4.0-5.0% (v/v), and cytokinin with the final concentration of 0.0045% -0.0055% (v/v);
the aquatic weed liquid fertilizer is 21g/L of potassium nitrate, 5g/L of urea, 30g/L of monopotassium phosphate, 41g/L of potassium sulfate, 8.053g/L of ferrous sulfate heptahydrate, 15.5g/L of magnesium sulfate, 1.66g/L of boric acid, 1.17g/L of manganese sulfate, 0.05g/L of copper sulfate pentahydrate, 0.255g/L of zinc sulfate heptahydrate, 15g/L of disodium diamine tetraacetate, 2g/L of L-ascorbic acid and 20g/L of biochemical potassium fulvate; the area of the epiphytic aquatic weed is 75-85% of the area of the upward surface of the fixed carrier;
the distance between the epiphytic aquatic weed and the surface of the fixed carrier which is close to the epiphytic aquatic weed is less than 2 mm.
2. The method of claim 1, wherein the agar solution is: agar at a final concentration of 19g/L, a liquid manure of aquatic weeds at a final concentration of 4.5% (v/v), and cytokinin at a final concentration of 0.005% (v/v).
3. The method for manufacturing the aquatic weed carrier is characterized in that the aquatic weed is a foreground aquatic weed;
the manufacturing method comprises the following steps: heating and dissolving the agar solution, and attaching the agar solution to a fixed carrier to form an agar jelly; directly covering the foreground aquatic weeds on the agar jelly, pressing the foreground aquatic weeds to be close to the fixed carrier, and cooling and solidifying to form the aquatic weeds carrier;
the agar solution is: agar with the final concentration of 16-20 g/L, a water-grass liquid fertilizer with the final concentration of 4.5-5.5% (v/v), cytokinin with the final concentration of 0.0045% -0.0055% (v/v) and biochemical fulvic acid potassium with the final concentration of 0.0405% -0.0495% (v/v);
the aquatic weed liquid fertilizer is 21g/L of potassium nitrate, 5g/L of urea, 30g/L of monopotassium phosphate, 41g/L of potassium sulfate, 8.053g/L of ferrous sulfate heptahydrate, 15.5g/L of magnesium sulfate, 1.66g/L of boric acid, 1.17g/L of manganese sulfate, 0.05g/L of copper sulfate pentahydrate, 0.255g/L of zinc sulfate heptahydrate, 15g/L of disodium diamine tetraacetate, 2g/L of L-ascorbic acid and 20g/L of biochemical potassium fulvate;
the area of the foreground aquatic weeds is 75% -85% of the area of the upward surface of the fixed carrier;
the distance between the foreground aquatic weed and the surface of the fixed carrier to which the foreground aquatic weed is close to is less than 2 mm.
4. The method of claim 3, wherein the agar solution is: agar with the final concentration of 18g/L, waterweed liquid fertilizer with the final concentration of 5% (v/v), cytokinin with the final concentration of 0.005% (v/v), and biochemical fulvic acid potassium with the final concentration of 0.045% (v/v).
5. A method for manufacturing a float grass carrier, which is characterized in that,
the aquatic weeds are tuber aquatic weeds;
the manufacturing method comprises the following steps: heating and dissolving the agar solution, and attaching the agar solution to a fixed carrier to form an agar jelly; removing leaves of the tuber aquatic weed, only reserving 1-2 cm leaf stalks, immersing tubers of the tuber aquatic weed into the agar jelly to ensure that growth points of new leaves of the tuber aquatic weed are exposed out of the agar jelly, and cooling and solidifying to form the tuber aquatic weed carrier;
the agar solution is: agar with the final concentration of 14.5-17.5 g/L, a water-grass liquid fertilizer with the final concentration of 4.5-5.5% (v/v), cytokinin with the final concentration of 0.0045-0.0055% (v/v), biochemical fulvic acid potassium with the final concentration of 0.0405-0.0495% (v/v), and an ultramarine high-potassium formula slow release fertilizer with the final concentration of 9-11 g/L;
the aquatic weed liquid fertilizer is 21g/L of potassium nitrate, 5g/L of urea, 30g/L of monopotassium phosphate, 41g/L of potassium sulfate, 8.053g/L of ferrous sulfate heptahydrate, 15.5g/L of magnesium sulfate, 1.66g/L of boric acid, 1.17g/L of manganese sulfate, 0.05g/L of copper sulfate pentahydrate, 0.255g/L of zinc sulfate heptahydrate, 15g/L of disodium diamine tetraacetate, 2g/L of L-ascorbic acid and 20g/L of biochemical potassium fulvate;
the ultramarine high-potassium formula slow release fertilizer is a conventional 328S type precision ultramarine fertilizer sold in the market.
6. The method of claim 5, wherein the agar solution is: agar with the final concentration of 16g/L, a waterweed liquid fertilizer with the final concentration of 5% (v/v), cytokinin with the final concentration of 0.005% (v/v), biochemical fulvic acid potassium with the final concentration of 0.045% (v/v), and an ultramarine high-potassium formula slow-release fertilizer with the final concentration of 10 g/L.
7. A method for manufacturing a float grass carrier, which is characterized in that,
the aquatic weeds are water weeds with stems;
the manufacturing method comprises the following steps: heating and dissolving the agar solution, and attaching the agar solution to a fixed carrier to form an agar jelly; removing the top, the bottom and original leaves of the stemmed aquatic weeds, then cutting the stemmed aquatic weeds into a plurality of stemmed aquatic weeds sections with the lengths of 6-8 cm, putting the stemmed aquatic weeds on the agar jelly, naturally immersing the stemmed aquatic weeds in the agar jelly, and cooling and solidifying to form the stemmed aquatic weed carrier;
the agar solution is: agar with the final concentration of 12.5-15.5 g/L, a water-grass liquid fertilizer with the final concentration of 4.5-5.5% (v/v), cytokinin with the final concentration of 0.0045-0.0055% (v/v), biochemical fulvic acid potassium with the final concentration of 0.0405-0.0495% (v/v), and an ultramarine high-potassium formula slow release fertilizer with the final concentration of 9-11 g/L;
the aquatic weed liquid fertilizer is 21g/L of potassium nitrate, 5g/L of urea, 30g/L of monopotassium phosphate, 41g/L of potassium sulfate, 8.053g/L of ferrous sulfate heptahydrate, 15.5g/L of magnesium sulfate, 1.66g/L of boric acid, 1.17g/L of manganese sulfate, 0.05g/L of copper sulfate pentahydrate, 0.255g/L of zinc sulfate heptahydrate, 15g/L of disodium diamine tetraacetate, 2g/L of L-ascorbic acid and 20g/L of biochemical potassium fulvate;
the ultramarine high-potassium formula slow release fertilizer is a commercially available accurate ultramarine high-potassium fertilizer with a conventional 328S model;
the stemmed aquatic weed segments covered 50% of the area of the surface of the agar jelly.
8. The method of claim 7, wherein the agar solution is: agar with the final concentration of 14g/L, a waterweed liquid fertilizer with the final concentration of 5% (v/v), cytokinin with the final concentration of 0.005% (v/v), biochemical fulvic acid potassium with the final concentration of 0.045% (v/v), and an ultramarine high-potassium formula slow-release fertilizer with the final concentration of 10 g/L.
9. The aquatic weed carrier produced by the method for producing an aquatic weed carrier according to any one of claims 1 to 8.
10. The aquatic weed carrier of claim 9, further comprising a cultivation process after the aquatic weed carrier is manufactured, wherein the cultivation process comprises:
(a) carrying out first-stage culture on the aquatic weed carrier until the aquatic weed grows out a new bud;
the culture conditions of the first stage culture include: the air humidity is 85-100%, the temperature is 20-35 ℃, the illumination intensity is 10000-30000 LX, and the illumination time is more than 8h per day;
(b) performing second-stage culture on the aquatic weed carrier;
the culture conditions of the second stage culture include: the air humidity is 80-90%, the temperature is 25-35 ℃, the illumination intensity is 20000-50000 LX, and the illumination time is more than 10h per day;
spraying water twice a day, and spraying 0.5% potassium dihydrogen phosphate solution once every three days after the water is sprayed;
(c) carrying out third-stage culture on the aquatic weed carrier;
the culture conditions of the third stage culture include: the air humidity is 80-90%, the temperature is 25-35 ℃, the illumination intensity is 20000-50000 LX, and the illumination time is more than 10h per day;
managing the aquatic weeds, wherein the rules of management comprise: removing the top end of the ludwigia octovalvis exuberant in leaf growth, and spraying cytokinin on the ludwigia octovalvis exuberant in leaf growth;
(d) carrying out fourth-stage culture on the aquatic weed carrier;
the culture conditions of the fourth stage culture include: the air humidity is 50% -70%, the illumination intensity is 10000-30000 LX, and the illumination time is 6-8 h every day;
watering every other day.
CN201710569585.2A 2017-07-12 2017-07-12 Aquatic weed carrier, its preparation method and special agar solution Expired - Fee Related CN107266193B (en)

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