CN107254504A - A kind of method that microbial bacterial agent improves lamp-dish flower acetic content - Google Patents
A kind of method that microbial bacterial agent improves lamp-dish flower acetic content Download PDFInfo
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- CN107254504A CN107254504A CN201710403066.9A CN201710403066A CN107254504A CN 107254504 A CN107254504 A CN 107254504A CN 201710403066 A CN201710403066 A CN 201710403066A CN 107254504 A CN107254504 A CN 107254504A
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P39/00—Processes involving microorganisms of different genera in the same process, simultaneously
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
- C12P17/02—Oxygen as only ring hetero atoms
- C12P17/06—Oxygen as only ring hetero atoms containing a six-membered hetero ring, e.g. fluorescein
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Abstract
The present invention relates to a kind of method that microbial bacterial agent improves lamp-dish flower acetic content, belong to Chinese medicine technical field of microbe application.The method be characterized in that the endophyte A Shi Bacillus strains and fusarium species strain that are obtained using being separated out of wild fleabane flower plant, carry out artificial liquid state fermentation and prepare 2 plants of endophyte microbial inoculums.Fleabane flower herb powder is first subjected to pretreatment sterilizing or microbial inoculum is directly added into, then 2 plants of microbial inoculums are proportionally added into fleabane flower herb powder aqueous solution, scutellarin transformation fermentation is carried out.2 kinds of microbial inoculums can be using fleabane flower herb powder or using the residue for extracting scutellarin, and microbe conversion obtains scutellarin, so as to improve the pick-up rate of scutellarin, the control for being relatively not added with microbial inoculum improves more than 20%.This provides new approach to improve effective medicinal ingredient scutellarin in fleabane flower, also alleviates Chinese medicine and strives ground with grain, with important economy and application value.
Description
Technical field
The present invention relates to a kind of method that microbial bacterial agent improves lamp-dish flower acetic content, belong to Chinese medicine microbial technique
Application field.
Background technology
Fleabane flower [Erigeron breviscapus(Vaniot) Hand.Mazz] category composite family (Compositae) bitter fleabane
Category (Erigeron), also known as erigeron breviscapus, Erigeron breviscapus, perennial wild herb originate in Yunnan, Hunan, Guangxi, expensive
The provinces and regions such as state, Sichuan, Tibet, abound with Yunnan.Its mildly bitter flavor cold in nature, Gan Wenxin, with radiating inducing diaphoresis, activate blood circulation and disperse blood clots, stimulate the menstrual flow
It is active, relax through Zhitan, dispelling wind and eliminating dampness, anti-inflammatory analgetic the effects such as, many index successively be repeatedly put into《People's Republic of China's medicine
Allusion quotation》In.Flavones and its Breviscapinun (oil lamp A prime, scutellarin) in fleabane flower, caffeoyl class compound is considered as lamp
The principle active component of small cup flower, has extensive pharmacological activity.Clinically fleabane flower be mainly used in treatment apoplexy, treatment cerebral infarction,
Outside angina pectoris and coronary heart disease, in the treatment for being also applied to diabetes, nephrosis, geriatric disease etc., with preferable curative effect, at present
One of required medicines as treatment cardiovascular and cerebrovascular disease, are classified as national Chinese medicine protection kind, have in pharmaceutical industry wide
Application prospect.
The acquisition of scutellarin in fleabane flower is main from fleabane flower herb, and research report improves scutellarin at present
Method, mainly the field production stage is using different cultivation step or uses different breeding methods.Such as Zhang Lei
Et al. induced by methyl jasmonate (MeJA) in field, realize the quick purpose for improving scutellarin content.Su Wen
China et al. finds that the difference of N supplys in soil is to cause the conjunction of the phenols such as flavones secondary metabolism active ingredient in medicinal plant body
Into the different one of the main reasons of accumulation.Yang Sheng superfine people research shows flavones content in fleabane flower mainly by the shadow of genotype
Ring, and the influence of environment occupies a secondary and subordinate position.Microbe particularly endophyte come improve the research of fleabane flower flavones content compared with
Few, Shen Rui et al. carries out seed treatment using a variety of endophyte microbial inoculums of fleabane flower, improves the biological yield and lamp of fleabane flower
Small cup B prime content.
Though fleabane flower has realized extensive artificial growth now, still lack improved seeds and more ripe cultivation technique,
Seminal propagation coefficient is low, and price is high, and cultivation technique difficulty is big, and biological yield and active constituent content are low, and with grain and warp
The problem of Ji crop " strive ground ", all seriously constrain the development of fleabane flower, the yield of fleabane flower and Breviscapinun far can not expire
The sufficient market demand.Meet market to the demand of scutellarin in addition to fleabane flower cultivated area and seed selection improved seeds are expanded, such as
What improves the content of oil lamp element in fleabane flower, is a current important matter of science and technology for facing and needing to solve.
Some endophytes of medicinal plant can produce the compound as plant, and this laboratory is in early-stage Study oil lamp
A collection of endogenetic bacteria and fungi are obtained during flower, some of which bacterial strain can utilize fleabane flower herb powder or utilize and carry
The residue of scutellarin was taken, conversion obtains scutellarin, so as to improve the pick-up rate of scutellarin.This is raising fleabane flower
In the effectively medicinal ingredient such as scutellarin provide new approach, also provide effective method with striving to solve medicine grain.
Current production and research far can not meet demand of the market to fleabane flower and scutellarin, extract fleabane flower
Primary medicinal component Flavonoid substances（Scutellarin）Technical process in, the Huang of fleabane flower is improved using microbial fermentation
The rarely seen research report of technical method of ketone content, this is a kind of method and approach for solving current fleabane flower Problems, is had
Important economy and application value.Medicine production is carried out using medicinal plant endophyte resource simultaneously, wild preciousness is also beneficial to
The protection of resources of medicinal plant, with important ecological significance.
The content of the invention
It is an object of the invention to solve the problem of above-mentioned prior art is present to utilize after fleabane flower harvest there is provided one kind
Fleabane flower endophyte improves the method and approach of Breviscapinun content, with more preferable economic benefit and ecological significance.
The invention mainly comprises following steps：
Present invention selection separates the 2 plants of endophytes obtained out of wild fleabane flower plant, and bacterium is A Shi bacillus J-6（Bacillus aryabhattaiJ-6）Bacterial strain, the bacterial strain is deposited in Chinese Typical Representative culture on October 17th, 2013
Thing collection, deposit number is CCTCC NO：M2013475.Fungal bacterial strain B-11 be fusarium (Fusariumsp.),
Now it is stored in institute of microbiology of Yunnan University.
Culture medium prescription：
Potato culture：Potato 200g, sucrose 20g, agar 15g, water 1000ml, PH are natural.
LB culture mediums：Peptone 10.0g, yeast extract 5.0g, Nacl5g, agar 15g, PH7.0, water 1000ml.
1. it is prepared by microbial inoculum：
A Shi Bacillus and fusarium strain are taken respectively, aseptically（Desinfection chamber or superclean bench）, with connecing
Pin or the sterilizing a little strain of bamboo stick picking are planted, is transferred respectively in sterilized solid LB media and PDA culture medium test tube（15
×150mm）In, put in incubator 28 DEG C of activation cultures 2~3 days.
The activation slant strains of 2~3 days are taken out, aseptically, strain are respectively connected in the same fashion to having sterilized
Seed LB liquid and PDA liquid medium in, the shaking table culture 2~5 days under 28 DEG C, rotating speed 120rpm~200rpm, be
A Shi bacillus J-6 strain fermentations seed liquor and fusarium B-11 strain fermentation seed liquors.
Fermented using 250ml Erlenmeyer flasks, be respectively charged into LB fluid nutrient mediums and PDA liquid medium 100ml, 121
After DEG C sterilizing 30min, take out and 28 DEG C of incubators are placed after cooling stay overnight, no living contaminants can confirm that sterilizing is thorough, can be used for
Subsequent fermentation culture.Aseptically, A Shi bacillus J-6 seed liquors and Fusarium B-11 seed liquors are taken respectively, point
It is not inoculated in the Erlenmeyer flask equipped with LB fluid nutrient mediums, inoculum concentration is 5~20%（v/w）, put 28 DEG C of ventilation cultures 2~5
My god, wherein A Shi bacillus is cultivated 1~3 day, fusarium culture 2~5 days.
During fermentation, note fermenting cellar temperature change, check for contaminated bacteria phenomenon.
Microbial inoculum is preserved：2 plants of bacterium carry out artificial liquid state fermentation respectively, and fermentation is finished, collection zymotic fluid, and rotating speed 5000rpm~
The 15000 rpm centrifugation min of 10min~2, abandon supernatant, obtain thalline, every gram of thalline of A Shi bacillus containing 2.6 × 1013~
1015CFU thalline, fusarium is the polymer of mycelium and spore.2 kinds of bacterium thalline press 1g with preservative fluid respectively：10mL~30mL
It is made into microbial inoculum.Preservative fluid for thallus is 0.5% Tween 80-physiological saline.The microbial inoculum of preservation is diluted in proportion, distinguished again when using
It is added in the fleabane flower herb powder liquid for preparing fermentation.
Fleabane flower herb powder pre-treating：
By fleabane flower herb powder add water, can arbitrarily be stirred and be defined by suspension, using high-temperature sterilization (121 DEG C, 15~
30min) or ethanol 70%~75%（30~50min）Method sterilizes, or is directly added into microbial inoculum.
Fleabane flower herb powder ferments：
After the fleabane flower herb powder pre-processed using high-temperature sterilization is terminated, 2 kinds of microbial inoculums are put into, the ratio of microbial inoculum and sample is 1:
In proportion 1~50 between 3~20 (W/W), 2 plants of microbial inoculums:50~1 (W/W) are prepared, and have been added to fleabane flower herb powder aqueous solution
In, stir, fleabane flower herb powder and water ratio are 1~6：50 (W/W), are placed on 20~40 DEG C of progress 8h~7 of temperature
Its scutellarin transformation fermentation, fermentation process needs to vibrate or stirred.Bacteria fermentation within 72h can surpass without sterilizing
Crossing 72h needs to ferment again after sterilizing.
After 70%~75% alcohol pre-treatment fleabane flower herb powder, scutellarin is first extracted, then by scutellarin
Residue after extraction carries out ethanol volatilization 1hr~10hr, adds ratio 1~50 between 2 kinds of microbial inoculums and water by fermentation, 2 plants of microbial inoculums:50
~1, the ratio of microbial inoculum and sample is 3~35:100.It is seated in 25~30 DEG C of temperature and carries out 8h~7 day scutellarin conversion hair
Ferment, fermentation process needs to vibrate or stirred.
2 kinds of processing modes of the above can relatively compare the extraction content of substantially increase scutellarin.
Scutellarin is extracted：
After fermentation ends, progress is ultrasonically treated, and supersonic frequency is 10~50kHZ, and ultrasonic power is 10~40kW, ultrasonic extraction temperature
20~50 DEG C of degree, 10min~3hr.Again by filtering, centrifugation, fleabane flower aqueous extract is obtained.The residue of filtering is added 1~
70%~75% ethanol of 20 times of water, then carry out it is ultrasonically treated, supersonic frequency be 10~50kHZ, ultrasonic power be 10~
40kW, ultrasonic extraction temperature is 20~50 DEG C, processing time 10min~60min.Filtering, centrifugation, filtrate is incorporated to for the first time
Extract solution mixed.
Scutellarin assay reference《Chinese Pharmacopoeia》The scutellarin content assaying method of fleabane flower is carried out.The present invention
In control CK choose fleabane flower herb only handled without microbial inoculum, other processing routines are completely the same.
Specific embodiment
The present invention is further illustrated in following specific embodiment, this does not limit the scope of the invention.Not
In the case of departing from above-mentioned technological thought of the invention, according to ordinary skill knowledge and conventional means, make various replacements and
Change, all should be included within the scope of the invention.
Specific implementation 1
Test tubes of the A Shi gemma bar J-6 and fusarium B-11 on equipped with LB culture mediums and PDA culture medium is taken respectively（15×
150mm）Middle activation, puts culture 2~3 days in 28 DEG C of incubators.The bacterial strain activated is respectively connected to sterilized seed LB liquid
In body culture medium and PDA liquid medium, using the 250mL triangular flasks equipped with 100mL culture mediums, every bottle is inoculated with 3~5 rings,
28 DEG C, seed liquor is made in rotating speed 150rpm shaking table cultures 2~3 days.
The seed liquor of 2 plants of bacterium is enlarged fermentation, fluid nutrient medium and seed bacteria liquid culture by 5% inoculum concentration respectively
Base is identical, and fermentation condition is identical with preparation-seed liquor, at 28 DEG C, rotating speed 150rpm shaking table cultures 3 days, and fermentation is finished, and collects
Zymotic fluid, rotating speed 10000rpm centrifugation 3min, abandons supernatant, obtains thalline, and every gram of thalline contains 4.1 × 1013 CFU thalline, sickle
Spore is mould for mycelium and the polymer of spore.2 kinds of bacterium thalline press 1g with preservative fluid：20ml is made into the microbial inoculum that can be preserved, thalline
Preservative fluid is 0.5% Tween 80-physiological saline.
Fleabane flower herb powder 5g is taken, is put into 30mL water, 121 DEG C are carried out, 20min high-temperature sterilizations are cooled to 30 DEG C
It is put into 2mL mix bacterium agents.The ratio 1 of A Shi Bacillus and fusarium strain：15（W：W）, stir.It is not added with microbial inoculum
Processing for control, it is other processing operation it is identical.
It is placed on 28 DEG C, rotating speed 150rpm shaking table culture 96hr after fermentation ends, carry out ultrasonically treated, supersonic frequency is
50kHZ, ultrasonic power is 30kW, and ultrasonic extraction temperature is room temperature DEG C, 30min.Again by filtering, centrifugation, fleabane flower water is obtained
Extract solution.75% ethanol 30mL is added in residue, residue is submerged in, then carries out ultrasonically treated, ultrasonic power is 30kW, is surpassed
Sound Extracting temperature is 28 DEG C, 30min.Filtering, centrifugation, the extract solution that filtrate is incorporated into first time are mixed.To the extract solution of acquisition
It is measured, the scutellarin content in the sample handled by microbial inoculum exceedes control 23.6%.
Specific implementation 2
The test tube of A Shi Bacillus and fusarium strain on equipped with LB culture mediums and PDA culture medium is taken respectively（15×
150mm）Middle activation, puts culture 2~3 days in 28 DEG C of incubators.The bacterial strain activated is respectively connected to sterilized seed LB liquid
In body and PDA liquid medium, using the 250mL triangular flasks equipped with 100mL culture mediums, every bottle of 3~5 rings of inoculation, at 28 DEG C, turn
Shaking table culture under fast 180rpm, A Shi bacillus is cultivated 2 days, and seed liquor is made in fusarium culture 4 days.
The seed liquor of 2 plants of bacterium is enlarged fermentation, fluid nutrient medium and seed bacteria liquid culture by 15% inoculum concentration respectively
Base is identical, and fermentation condition is identical with preparation-seed liquor, shaking table culture 3 days under 28 DEG C, rotating speed 200rpm, and fermentation is finished, and receives
Collect zymotic fluid, rotating speed 8000rpm centrifugation 5min abandon supernatant, obtain thalline, every gram of thalline of A Shi bacillus containing 2.9 ×
1014CFU thalline, fusarium is the polymer of mycelium and spore.2 kinds of bacterium thalline press 1g with preservative fluid：20ml is made into bacterium
Agent, preservative fluid for thallus is 0.5% Tween 80-physiological saline.
Fleabane flower herb powder 5g is weighed, by 1:10（W：W）Ratio be put into water, be put into microbial inoculum 3mL, A Shi gemma bar
The ratio 8 of bacterium strain and fusarium strain：25（W：W）, stir.It is control to be not added with the processing of microbial inoculum, other processing operations
It is identical.
It is placed on 28 DEG C, rotating speed 180rpm shaking table culture 72hr after fermentation ends, carry out ultrasonically treated, supersonic frequency is
30kHZ, ultrasonic power is 30kW, and ultrasonic extraction temperature is 28 DEG C, 50min.Again by filtering, centrifugation, fleabane flower water extraction is obtained
Take liquid.50mL75% ethanol is added in residue, being submerged in residue makes residue be readily able to rotation, then carries out ultrasonically treated.Cross
Filter, centrifugation, the extract solution that filtrate is incorporated into first time are mixed.
The extract solution of acquisition is measured, the scutellarin content in the sample handled by microbial inoculum is higher by control
24.7%。
Specific implementation 3
The test tube of A Shi Bacillus and fusarium strain on equipped with LB culture mediums and PDA culture medium is taken respectively（15×
150mm）Middle activation, puts culture 2~3 days in 28 DEG C of incubators.The bacterial strain activated is respectively connected to sterilized seed LB liquid
In body and PDA liquid medium, using the 250mL triangular flasks equipped with 100mL culture mediums, every bottle of 3~5 rings of inoculation, at 28 DEG C, turn
Shaking table culture under fast 180rpm, A Shi bacillus is cultivated 2~3 days, and seed liquor is made in fusarium culture 3~4 days.
The seed liquor of 2 plants of bacterium is enlarged fermentation, fluid nutrient medium and seed army Liquid Culture by 15% inoculum concentration respectively
Base is identical, and fermentation condition is identical with preparation-seed liquor, shaking table culture 3 days under 28 DEG C, rotating speed 200rpm, and fermentation is finished, and receives
Collect zymotic fluid, rotating speed 8000rpm centrifugation 5min abandon supernatant, obtain thalline, every gram of thalline of A Shi bacillus containing 2.9 ×
1014CFU thalline, fusarium is the polymer of mycelium and spore.2 kinds of bacterium thalline press 1g with preservative fluid：20ml is made into bacterium
Agent, preservative fluid for thallus is 0.5% Tween 80-physiological saline.
Fleabane flower herb powder 5g is weighed, by 1:8（W：W）Ratio be put into 70% ethanol, in 28 DEG C, rotating speed
Shaking table vibrates 1hr under 200rpm, and progress is ultrasonically treated, and supersonic frequency is 30kHZ, and ultrasonic power is 30kW, ultrasonic extraction temperature
For 28 DEG C, 30min.Again by filtering, centrifugation, extract solution is obtained.
Residue is placed on ventilation 1hr, 2 kinds of microbial inoculum 3mL, A Shi Bacillus and fusarium strain is added
Ratio 3：20（W：W）, stir.It is control to be not added with the processing of microbial inoculum, and other processing operations are identical.28 DEG C are placed on,
After rotating speed 150rpm shaking table culture 72hr, fermentation ends, progress is ultrasonically treated, and supersonic frequency is 30kHZ, and ultrasonic power is
30kW, ultrasonic extraction temperature is room temperature, 40min.Again by filtering, centrifugation, second of fleabane flower aqueous extract is obtained.Survey respectively
The scutellarin content of fixed No. 2 extract solutions.The scutellarin content of the sample handled by microbial inoculum exceedes control 28.4%.
Claims (3)
1. a kind of method that microbial bacterial agent improves lamp-dish flower acetic content, including prepared by microbial inoculum, fleabane flower herb powder is located in advance
Reason, the fermentation of fleabane flower herb powder, scutellarin are extracted, it is characterised in that：
1. prepared by microbial inoculum, and A Shi Bacillus strains and fusarium strain are taken respectively, aseptically, is transferred respectively in having gone out
The solid LB media and PDA culture medium activated strains of bacterium, then using fluid nutrient medium, at 28 DEG C, rotating speed 120rpm~
Shaking table culture 2~5 days under 200rpm, prepare A Shi bacillus J-6 bacterial strains and fusarium B-11 strain fermentation seed bacterium solutions and
Preservation microbial inoculum；2. fleabane flower herb powder pre-treating, adds water by fleabane flower herb powder, is defined, adopted by can arbitrarily stir
With high-temperature sterilization (121 DEG C, 15~30min) or ethanol 70%~75%（30~50min）Method sterilizes, or is directly added into bacterium
Agent；3. fleabane flower herb powder ferments, and 2 kinds of microbial inoculums are added in fleabane flower herb powder aqueous solution, stir, microbial inoculum with
The ratio of sample is 3~35:100 (W/W) are prepared, and fleabane flower herb powder and water ratio are 1~6：50 (W/W), are placed on
20~40 DEG C of progress 8h~7 day scutellarin transformation fermentations of temperature, fermentation process needs to vibrate or stirred；
4. scutellarin is extracted, after fermentation ends, and progress is ultrasonically treated, then by filtering, centrifugation, obtains the extraction of fleabane flower water
Liquid, then the residue after filtering is added into 70%~75% ethanol, then ultrasonically treated, centrifugation is carried out, filtrate is incorporated to carrying for first time
Liquid is taken to detect or detect respectively.
2. the method according to claim 1 for improving lamp-dish flower acetic content, it is characterised in that：2 plants of endophytes Ah
Family name bacillus J-6（ Bacillus aryabhattai J-6）With fusarium B-11 ( FusariumSp.) bacterial strain from
Separation is obtained inside wild fleabane flower plant, after microorganism fluid zymotechnique individually fermentation, in proportion 1 between 2 plants of microbial inoculums
~50:50~1 (W/W) are prepared, well mixed to use.
3. the application of the A Shi bacillus J-6 and fusarium B-11 described in claim 1 or 2, it is characterised in that this 2 plants of bacterium
Strain is as preparation is for fleabane flower herb or extracts residue fermentation, the application of final raising lamp-dish flower acetic pick-up rate.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112980913A (en) * | 2021-04-14 | 2021-06-18 | 北京欣康研医药科技有限公司 | A method for preparing baicalein from baicalin by fermentation |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102154123A (en) * | 2011-03-16 | 2011-08-17 | 都晓伟 | Dioscorea nipponica Makino Fusarium sp. and application thereof |
| CN103477837A (en) * | 2013-09-17 | 2014-01-01 | 云南大学 | Erigeron breviscapus cultivating method for increasing content of breviscapinum |
| CN103614319A (en) * | 2013-11-12 | 2014-03-05 | 云南省烟草公司曲靖市公司 | Bacillus aryabhattai and application thereof in preventing and treating tobacco black shank |
| CN105063156A (en) * | 2015-05-29 | 2015-11-18 | 云南大学 | New method for enriching rubrosterone through fermentation modification on Asparagus filicinus root tuber with Fusarium oxysporum |
| WO2017049353A1 (en) * | 2015-09-24 | 2017-03-30 | Agriculture Victoria Services Pty Ltd | Bioactive fungi |
-
2017
- 2017-06-01 CN CN201710403066.9A patent/CN107254504A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102154123A (en) * | 2011-03-16 | 2011-08-17 | 都晓伟 | Dioscorea nipponica Makino Fusarium sp. and application thereof |
| CN103477837A (en) * | 2013-09-17 | 2014-01-01 | 云南大学 | Erigeron breviscapus cultivating method for increasing content of breviscapinum |
| CN103614319A (en) * | 2013-11-12 | 2014-03-05 | 云南省烟草公司曲靖市公司 | Bacillus aryabhattai and application thereof in preventing and treating tobacco black shank |
| CN105063156A (en) * | 2015-05-29 | 2015-11-18 | 云南大学 | New method for enriching rubrosterone through fermentation modification on Asparagus filicinus root tuber with Fusarium oxysporum |
| WO2017049353A1 (en) * | 2015-09-24 | 2017-03-30 | Agriculture Victoria Services Pty Ltd | Bioactive fungi |
Non-Patent Citations (2)
| Title |
|---|
| 何永美等: "灯盏花产黄酮内生真菌的筛选及其产黄酮能力的初步研究", 《农业环境科学学报》 * |
| 李治滢等: "灯盏细辛内生真菌的研究I：菌种分离及其分类鉴定", 《云南大学学报(自然科学版)》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112980913A (en) * | 2021-04-14 | 2021-06-18 | 北京欣康研医药科技有限公司 | A method for preparing baicalein from baicalin by fermentation |
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