CN104342388B - Streptomycete bacterial strain and prevent and treat tomato wilt use in conjunction - Google Patents
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Abstract
The invention discloses streptomycete bacterial strain and prevent and treat tomato wilt use in conjunction, relate to microbial technology field, actinomycetes of the present invention are to use actinomycetes isolation technics to separate from 10 ~ 15 cm deep layer soil samples that salt-soda soil, suburb, Tai'an, Shandong Province gathers to obtain, identify through microbial taxonomy, named streptomyces rimosus (Streptomyces rimosus) M527 and styreptomyces globispotus strain (Streptomyces globisporus) M927,S. rimosus 2013 are preserved in China typical culture collection center on June 19th, 2013, and deposit number is: CCTCC NO:M 2013270；S. globisporusM927 is preserved in China typical culture collection center on 25th in December in 2013, and deposit number is: CCTCC NO:M 2013709.Two bacterial strains all can ferment produce to tomato wilt bacterium (Fusarium oxysporumF. sp. Lycopersici Snyder et Hansen) there is the active substance of stronger antagonism, can be used for preventing and treating the plant diseases such as tomato wilt.When the tunning percentage by weight of two bacterial strains is 4:1 7:3 mixing, biocontrol effect is especially pronounced when using respectively as unitary agent compared with two kinds of metabolites.
Description
Technical field
The present invention relates to microbial technology field, relate to two strain biocontrol actinomycetes streptomyces rimosus M527 and ball spore strepto-s
The application in preventing and treating tomato wilt of the bacterium M927 metabolite.
Technical background
Fructus Lycopersici esculenti (Lycopersicon esculentum Mill) it is a kind of global vegetable crop, the master of Ye Shi China
Plant one of vegetable.Tomato wilt is one of Major Diseases of Fructus Lycopersici esculenti, and this disease infectiousness is strong, has had a strong impact on the yield of Fructus Lycopersici esculenti
And quality.
The pathogen of tomato wilt is Fusarium oxysporum specialized formFusarium oxysporum f. sp.
Lycopersici Snyder et Hansen, the specialization of tomato wilt bacterium is extremely strong, only endangers Fructus Lycopersici esculenti.Source of disease is Fungi Imperfecti
Subphylum, the Fructus Lycopersici esculenti wilting germ of Fusarium.Pathogenic bacteria survives the winter in soil, and large bamboo hat with a conical crown and broad brim year is with rainwater, irrigation water and soil-borne.Pathogen
Immerse from young root or wound, extend in plant dimension pipe card and spread, obstruction conduit, and produce the wilting element of poisonous Fructus Lycopersici esculenti, with defeated
Lead tissue development, cause vascular bundle browning, lose transporting function, thus cause plant to wither.For tomato wilt evil at present
Pathogen killed by main employing chemical pesticide, and a large amount of life-time service of these medicaments not only make tomato plant develop immunity to drugs,
Pesticide residues also can affect the quality of Fructus Lycopersici esculenti, brings hidden danger to agricultural product security.Therefore, Biological control causes the attention of people.
There are some researches show the active component in plant extract at present to there being certain inhibitory action, but effective ingredients in plant extracts operation
Step is many, and efficiency is low；Current research is concentrated mainly on and utilizes the metabolite of microorganism to prevent and treat tomato wilt bacterium, Qi Zhongtong
Green pseudomonas, Fusarium spp. have potential pathogenic as antagonistic microbe, though separation screening bacillus cereus has wide coverage,
But the poor stability of bacterial strain own, prevention effect is the most notable.Actinomycetes are easily generated anti-microbial pathogen composition isoreactivity material because of it,
Become Main Resources and the main source of biological pesticide exploitation of Biological control plant pest.Soil is the weight of actinomycetes perch
Want place, from soil, obtain that to have the actinomycetes of high-efficiency antimicrobial activity be to develop new medicine and new in current world wide
The requisite element task of pesticide, but from soil, screen the Biological control report of Antagonistic Actinomycetes bacterium former to tomato wilt
The most actually rare.
Summary of the invention
In order to overcome the deficiencies in the prior art, first purpose of the present invention is to be provided tomato wilt by screening
Former bacterium has two strain biocontrol microorganisms bacterium streptomyces rimosus M527 and styreptomyces globispotus strain M927 of stronger antagonism.
Second object of the present invention is to be mixed the tunning of two strain biological and ecological methods to prevent plant disease, pests, and erosion streptomycetes by different ratios, it is provided that on
State bacterium streptomyces rimosus and the styreptomyces globispotus strain use in conjunction in tomato wilt is prevented and treated.
It is an object of the invention to be achieved by techniques below means:
The present invention first with the soil sample that gathers in salt-soda soil, suburb, Tai'an, Shandong Province as object of study, separated, fermentation
Cultivate, broth extraction and the steps such as plant pathogenic fungi inhibitory activity detection are obtained two strains bacterium former to tomato wilt tool
There is the streptomycete of stronger bacteriostatic activity；Wherein a strain is identified and named streptomyces rimosus through microbial taxonomy
(Streptomyces rimosus) M527.This bacterium is preserved in China typical culture collection center on June 19th, 2013,
Deposit number is: CCTCC NO:M 2013270；Another strain is identified and named styreptomyces globispotus strain through microbial taxonomy
(Streptomyces globisporus) M927, this bacterium is preserved in China typical culture collection on 25th in December in 2013
Center, deposit number is CCTCC NO:M 2013709.China typical culture collection center address: wuchang, wuhan Luo Ka
Mountain, postcode: 430072.
Streptomyces rimosus of the present invention (Streptomyces rimosus) M527 cultivates on PDA solid medium
Seasonal epidemic pathogens silk white is to Lycoperdon polymorphum Vitt, base silk creamy.Microexamination findsStreptomyces rimosus It is threaded at the beginning of M527 fibrillae of spores
Spiral type.Spore Long Circle is to cylindricality, smooth surface.Of the present inventionStreptomyces rimosus M527, fermentation liquid
Tomato wilt pathogenic fungi can be had stronger inhibitory action after extracted concentration.
Styreptomyces globispotus strain of the present invention (Streptomyces globisporus) M927 is on PDA solid medium
Cultivating seasonal epidemic pathogens silk dark straw grass color, base silk is colourless.Microexamination findsStreptomyces globisporusM927 spore filament length
Branch shape, spore oval, smooth surface.Of the present inventionStreptomyces globisporusM927, fermentation liquor
Extract and tomato wilt pathogenic fungi can be had stronger inhibitory action after concentrating.
The screening of described tomato wilt former bacterium Antagonistic Actinomycetes and bacteriostatic activity detection process are as described below:
(1) gather soil sample from field (salt-soda soil, suburb, Tai'an, Shandong Province), and carry out drying process；
(2) separating payingoff bacteria from soil sample, and utilize the former bacterium of tomato wilt to have antagonism as indicator bacteria screening
Actinomycetes；
(3) by the actinomycetes grown dibbling respectively in being mixed with tomato wilt former bacterium spore suspension (1 × 106 cfu·mL-1) PDA plate on, by observing inhibition zone size, select the obvious bacterial strain of inhibition zone and enter multiple sieve；
(4) bacterial strain chosen being carried out fermentation culture, after fermentation ends, centrifugal collection fermentation liquid, is extracted with ethyl acetate,
Preparing extractum after vacuum-concentrcted, the preventive effect for tomato wilt detects, and thus filters out preferable two strains of prevention effect
Antagonistic microbe streptomyces rimosus M527 and styreptomyces globispotus strain M927.
Beneficial effects of the present invention:
The present invention separates bacterium former to tomato wilt from soil and has two strain antagonism chains of relatively high inhibition effect
MyceteStreptomyces rimosus M527 andStreptomyces globisporusM927, the fermentation of two strain bacterial strains is produced
The mix preparation that thing is made constantly for 4:1 7:3 by weight percentage can be used for the preventing and treating of tomato wilt disease, for biological agriculture
The exploitation of medicine provides new approach.
Detailed description of the invention
Embodiment 1:(Streptomyces rimosus M527 andStreptomyces globisporusM927 divides
From with screening)
Sequentially include the following steps:
(1) material and culture medium: for separating the material of streptomyces rimosus M527 and styreptomyces globispotus strain M927 for picking up from mountain
The soil sample in salt-soda soil, suburb, Dong Sheng Tai'an, is the PDA culture medium containing potassium dichromate for the culture medium used by actinomycetes separation
(formula is: weigh 200g Rhizoma Solani tuber osi, cleans peeling chopping, adds distilled water 1L and boil half an hour, filtered through gauze, then adds 20g glucose
Sugar and 20g agar, filtered through gauze while hot after fully dissolving, in subpackage to conical flask or teat glass, 121 DEG C, autoclaving
20min；Potassium dichromate 20 μ g mL is added during use-1；In tests below, actinomycetes cellar culture used medium is all to steam
Distilled water configures)；
(2) isolated and purified: the soil sample of above-mentioned fresh collection being loaded in sampler bag, after taking back laboratory, subpackage dries one week
Left and right；Soil sample every part after drying weighs 5g, is separately added in the conical flask containing 45ml sterilized water, adds 3-5 in conical flask
The bead that grain is aseptic, 28 DEG C of shaking tables are cultivated 2h, are made the microorganism in soil sample well in water；Draw cultivate after sample with
10 times of gradient dilutions, take 10 respectively-2、10-3、10-4Concentration dilution liquid 200 μ L, coats containing 20 μ g mL-1The PDA of potassium dichromate
On solid plate, cultivate 72h for 28 DEG C；The actinomycetes picking list bacterium colony respectively that will grow on flat board, is inoculated in containing potassium dichromate
On fresh PDA flat board, under the conditions of 28 DEG C ± l DEG C, purification cultivates 72h, it is thus achieved that actinomycetes after purification；
(3) primary dcreening operation and multiple sieve: by the dibbling respectively of the actinomycetes of isolated in being mixed with tomato wilt former bacterium spore suspension
(1×106cfu·mL-1) PDA solid plate on, by observe inhibition zone size, select the obvious bacterial strain of inhibition zone and carry out
Multiple sieve；
Multiple sieve uses flat board face-off method that the actinomycetes of primary dcreening operation are carried out Antibacterial Activity, method particularly includes: at PDA solid
It is respectively symmetrically on flat board and places the actinomycetes bacterium cake of a diameter 4mm and a tomato wilt former bacterium bacterium cake, put in incubator
Cultivate 72h under the conditions of 28 DEG C ± l DEG C, process as comparison using sterilized water, be repeated 3 times；Decussation method is used to measure bacterium colony straight
Footpath.Bacteriostasis rate is calculated by below equation:
Bacteriostasis rate (%)=(1-processes colony growth radius/comparison colony growth radius) × 100
Obtaining the preferable two strain actinomycetes of bacteriostatic activity according to result, the bacteriostasis rate of bacterium former to tomato wilt is all 75%
Above.The comprehensive comparison result of 16SrDNA sequence, morphological characteristic, cultural characteristic and physio-biochemical characteristics, wherein a strain bacterial strain mirror
It is set to streptomyces rimosus, named streptomyces rimosus M527(Streptomyces rimosusM527)；Another strain identification of strains
For styreptomyces globispotus strain, named styreptomyces globispotus strain M927(Streptomyces globisporusM927)；Preserve.
(4) Streptomyces rimosus M527 andStreptomyces globisporusM929 bacteriostatic activity
Measure: potato dextrose broth will be utilized culturedStreptomyces rimosus M527 andStreptomyces globisporusM929 is by 5%(v/v) it is respectively connected in fermentation medium, under the conditions of 28 DEG C ± l DEG C,
180 r/min, after 120h cultivated by shaking table, 7800 r/min are centrifuged 7 min, obtain ferment filtrate with filter paper filtering.Learn from else's experience 0.22 μm
The fermented supernatant fluid 1mL of membrane filtration is in sterile petri dish, and the PDA culture medium being cooled to 50 DEG C with 9mL mixes rapidly, after cooling
Each culture medium plane central put respectively 1 a diameter of 4mm tomato wilt bacterium (Fusarium oxysporum f.
Sp. Lycopersici Snyder et Hansen) bacterium cake, put in incubator 28 DEG C and cultivate 72h, using sterilized water process as
Comparison, is repeated 3 times；Statistical result when about the 2/3 of comparison colony diameter length to plate diameter, uses decussation method to measure
Colony diameter, with following equation calculating suppression ratio:
Mycelial growth inhibition rate (%)=[(comparison colony diameter-process colony diameter)/(comparison colony diameter-4)] ×
100
Measurement result shows:Streptomyces rimosus M527 andStreptomyces globisporus M927
Metabolite is respectively 77.1% and 75.0% to tomato wilt bacterium mycelial growth inhibition rate.
Embodiment 2:(Streptomyces rimosus M527 andStreptomyces globisporusM927 ferments
The preparation method of metabolite)
Sequentially include the following steps:
(1) activation culture of strain: by preserveStreptomyces rimosus M527 andStreptomyces globisporusM927 spore suspension (1 × 108 cfu·mL-1) access potato dextrose broth, at 28 DEG C ± l
48h is cultivated for fermentation under the conditions of DEG C；
(2) fermentation culture: containing soybean cake powder 10g, Semen Maydis powder 4g, soluble starch 20g in every 1 L of fermentation medium,
Glucose 5g, peptone 5g, CoCl2 0.02g, Carnis Bovis seu Bubali cream 5g, CaCO3 5g, yeast extract 5g, the bottled fermentation training of every 300mL triangle
Nutrient solution 50mL；Prepare rear 121 DEG C of sterilizing 20min, potato dextrose broth will be utilized culturedStreptomyces rimosus M527 andStreptomyces globisporusM927 presses 5%(v/v respectively) access fermentation
In culture medium, under the conditions of 28 DEG C ± l DEG C, shaking speed 180 r/min, fermentation culture 84 h；
(3) fermented product extracts: ferment complete, centrifugal, abandons thalline, and collection fermentation liquid is in separatory funnel, by bodies such as additions
Long-pending ethyl acetate extraction, fully mixes, and after being layered, takes upper phase to round-bottomed flask, after utilizing Rotary Evaporators to concentrate
Obtain extract extractum, be used for measuring bacteriostatic activity.
Described in example 3 below and test exampleStreptomyces rimosus M527 andStreptomyces globisporusM927 metabolite is embodiment 2 products made thereby；The percentage composition of described product is mass/volume hundred
Proportion by subtraction.
Embodiment 3:(Streptomyces rimosus M527 andStreptomyces globisporusM927 metabolism
The product inhibitory action to tomato wilt bacterium)
(1) the most accurately weighStreptomyces rimosus M527 andStreptomyces globisporus
M927 metabolite 0.1g, and add the sterilized water of 10mL volume, ultrasonic wave concussion fully dissolves, and is respectively configured final concentration of 10
The mother solution of g/L；
(2) Antibacterial Activity: take above-mentioned mother solution and be diluted to the solution that concentration is 1g/L and 0.5g/L respectively, take each concentration
Solution 1mL in sterile petri dish, with 9mL be cooled to the PDA culture medium of 50 DEG C mix rapidly (Streptomyces rimosus
M527 andStreptomyces globisporusM927 metabolite final concentration is 0.1g/L and 0.05g/L respectively), cold
But put the tomato wilt bacterium bacterium cake of 1 a diameter of 4mm after respectively in each culture medium plane, bacterium cake is connected to culture dish central authorities
(a diameter of 9cm of culture dish), puts 28 DEG C of cultivation 36h in incubator, processes as comparison, weight using conventional chemical medicament and sterilized water
Multiple 3 times；Decussation method is used to measure colony diameter, with following equation calculating suppression ratio:
Mycelial growth inhibition rate (%)=[(comparison colony diameter-process colony diameter)/(comparison colony diameter-4)] ×
100
Test example: (product of the present invention and conventional pesticide prevention effect contrast test)
Result of the test is shown in Table l.Find out from table l,Streptomyces rimosus M527 andStreptomyces globisporusThe M927 metabolite suppression tomato wilt more conventional chemical agent of bacterium effect is the most excellent, when both metabolites
When being mixed and made into mix preparation according to 4:1 7:3 mass percent, the effect preventing and treating the former bacterium of tomato wilt is made respectively compared with both
For the most notable during unitary agent.
Table l Streptomyces rimosus M527 andStreptomyces globisporusM927 metabolite
To supplying the inhibition of the former bacterium of tomato wilt tried and comparing
"-" is not test (N.T.).
Claims (3)
1. streptomycete bacterial strain combination, it is characterised in that: bacterial strain M527 Classification And Nomenclature be streptomyces rimosus (Streptomyces rimosus) M527, depositary institution: China typical culture collection center, preservation day: on June 19th, 2013, preservation registration number
For CCTCC NO:M 2013270；Another kind of streptomycete bacterial strain M927, Classification And Nomenclature be styreptomyces globispotus strain (Streptomyces globisporus) M927, depositary institution: China typical culture collection center, preservation day: on December 25th, 2013, preservation is stepped on
Mark is CCTCC NO:M 2013709.
2. the application of a streptomycete bacterial strain as claimed in claim 1 combination, it is characterised in that: the be full of cracks strepto-described in utilization
Bacterium M527 and styreptomyces globispotus strain M927 carries out fermentation and prepares metabolite, prepares the metabolite weight hundred of M527 and M927
Proportion by subtraction is the mixing formula preparation of 4:1 7:3, is used for preventing and treating tomato wilt disease.
3. the application described in claim 2, it is characterised in that: described mixing formula formulation preparation method is as follows:
(1) the streptomyces rimosus M527 and styreptomyces globispotus strain M927 of isolated are coated the MS that mannitol soybean cake powder is made
On flat board, collect spore inoculating in potato dextrose broth；
(2) containing soybean cake powder 10g during fermentation medium is every 1 L, Semen Maydis powder 4g, soluble starch 20g, glucose 5g, egg
White peptone 5g, CoCl2 0.02g, Carnis Bovis seu Bubali cream 5g, CaCO3 5g, yeast extract 5g, every 300mL triangle bottled fermentation culture 50mL；Join
Make rear 121 DEG C of sterilizing 20min, potato dextrose broth cultured streptomyces rimosus M527 and ball spore chain will be utilized
Mycete M927 presses 5%(v/v respectively) access in fermentation medium, under the conditions of 28 DEG C ± l DEG C, shaking speed 180 r/min, sends out
Ferment cultivates 84 h；
(3) centrifugal collection fermentation liquid after fermentation ends, adds isopyknic ethyl acetate, fully shakes mixing, take upper strata, very
It is concentrated into extractum under empty condition；
(4) mix preparation preparation: by streptomyces rimosus M527 and styreptomyces globispotus strain M927 metabolite water dissolution, mixing, join
Final concentration of 0.08g/L streptomyces rimosus M527 metabolite and the mixing of 0.02g/L styreptomyces globispotus strain M927 metabolite is become to join
Square preparation.
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| CN105349455B (en) * | 2015-11-09 | 2018-10-12 | 中国热带农业科学院环境与植物保护研究所 | One plant of Malaysian streptomycete and its application |
| CN110791458B (en) * | 2019-12-04 | 2022-07-15 | 河南农业大学 | Biocontrol strain and biocontrol microbial inoculum for preventing and treating bitter gourd fusarium wilt as well as preparation method and application of biocontrol strain and biocontrol microbial inoculum |
| CN114058530A (en) * | 2020-08-04 | 2022-02-18 | 复旦大学 | Streptomyces macrolepis endophytic strain for producing mycochromene and preparation method thereof |
| CN112746037B (en) * | 2020-12-16 | 2022-12-13 | 西南林业大学 | Streptomyces castochromogenes strain CPAT-W03 and application thereof |
| CN116376708B (en) * | 2022-12-05 | 2024-06-07 | 青岛农业大学 | Cladosporium fungus and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101961014A (en) * | 2010-03-19 | 2011-02-02 | 中国计量学院 | Method for preparing microbial pesticide for preventing and controlling fungal diseases of vegetables |
| CN102911970A (en) * | 2012-09-23 | 2013-02-06 | 江苏丘陵地区镇江农业科学研究所 | Application of metabolic product of streptomyces corchorusii NF0919 bacterial strain to preventing and treating tomato diseases |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101961014A (en) * | 2010-03-19 | 2011-02-02 | 中国计量学院 | Method for preparing microbial pesticide for preventing and controlling fungal diseases of vegetables |
| CN102911970A (en) * | 2012-09-23 | 2013-02-06 | 江苏丘陵地区镇江农业科学研究所 | Application of metabolic product of streptomyces corchorusii NF0919 bacterial strain to preventing and treating tomato diseases |
Non-Patent Citations (1)
| Title |
|---|
| 防病促生功能性微生物的筛选及应用研究;王占武 等;《河北农业科学》;20040630(第2期);摘要 * |
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