CN107238645B - 在线监测用葡萄糖氧化酶丝网印刷电极及其制备方法 - Google Patents
在线监测用葡萄糖氧化酶丝网印刷电极及其制备方法 Download PDFInfo
- Publication number
- CN107238645B CN107238645B CN201710329483.3A CN201710329483A CN107238645B CN 107238645 B CN107238645 B CN 107238645B CN 201710329483 A CN201710329483 A CN 201710329483A CN 107238645 B CN107238645 B CN 107238645B
- Authority
- CN
- China
- Prior art keywords
- electrode
- enzyme
- mol
- pei
- screen printing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000007650 screen-printing Methods 0.000 title claims abstract description 26
- 235000019420 glucose oxidase Nutrition 0.000 title claims abstract description 17
- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- 108010015776 Glucose oxidase Proteins 0.000 title claims abstract description 12
- 239000004366 Glucose oxidase Substances 0.000 title claims abstract description 12
- 229940116332 glucose oxidase Drugs 0.000 title claims abstract description 12
- 238000012544 monitoring process Methods 0.000 title claims abstract description 7
- 108090000790 Enzymes Proteins 0.000 claims abstract description 47
- 102000004190 Enzymes Human genes 0.000 claims abstract description 47
- 229940088598 enzyme Drugs 0.000 claims abstract description 47
- DCYOBGZUOMKFPA-UHFFFAOYSA-N iron(2+);iron(3+);octadecacyanide Chemical compound [Fe+2].[Fe+2].[Fe+2].[Fe+3].[Fe+3].[Fe+3].[Fe+3].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-] DCYOBGZUOMKFPA-UHFFFAOYSA-N 0.000 claims abstract description 35
- 239000013225 prussian blue Substances 0.000 claims abstract description 35
- 229960003351 prussian blue Drugs 0.000 claims abstract description 35
- 239000007788 liquid Substances 0.000 claims abstract description 25
- 238000004519 manufacturing process Methods 0.000 claims abstract description 23
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 21
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 21
- 238000000034 method Methods 0.000 claims abstract description 12
- 230000004048 modification Effects 0.000 claims abstract description 10
- 238000012986 modification Methods 0.000 claims abstract description 10
- 239000003365 glass fiber Substances 0.000 claims abstract description 5
- 239000000463 material Substances 0.000 claims abstract description 5
- 239000000872 buffer Substances 0.000 claims description 24
- 239000011734 sodium Substances 0.000 claims description 24
- 239000000243 solution Substances 0.000 claims description 23
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 22
- 229920002873 Polyethylenimine Polymers 0.000 claims description 21
- 230000015572 biosynthetic process Effects 0.000 claims description 14
- 238000003786 synthesis reaction Methods 0.000 claims description 14
- 239000007864 aqueous solution Substances 0.000 claims description 13
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 claims description 12
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 12
- 239000003960 organic solvent Substances 0.000 claims description 12
- 238000007639 printing Methods 0.000 claims description 12
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 claims description 10
- 238000006243 chemical reaction Methods 0.000 claims description 10
- 239000000203 mixture Substances 0.000 claims description 10
- 239000000758 substrate Substances 0.000 claims description 9
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 8
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims description 8
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 8
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 8
- 239000003822 epoxy resin Substances 0.000 claims description 8
- 239000004570 mortar (masonry) Substances 0.000 claims description 8
- 239000002245 particle Substances 0.000 claims description 8
- 229920000647 polyepoxide Polymers 0.000 claims description 8
- 229920000915 polyvinyl chloride Polymers 0.000 claims description 8
- 239000004800 polyvinyl chloride Substances 0.000 claims description 8
- 239000013049 sediment Substances 0.000 claims description 8
- 239000000725 suspension Substances 0.000 claims description 8
- 238000000108 ultra-filtration Methods 0.000 claims description 8
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims description 7
- 238000013019 agitation Methods 0.000 claims description 7
- 229940098773 bovine serum albumin Drugs 0.000 claims description 7
- 239000001768 carboxy methyl cellulose Substances 0.000 claims description 7
- 238000002156 mixing Methods 0.000 claims description 7
- -1 alkene imines Chemical class 0.000 claims description 5
- 239000000126 substance Substances 0.000 claims description 5
- 229960000583 acetic acid Drugs 0.000 claims description 4
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 claims description 4
- 239000000853 adhesive Substances 0.000 claims description 4
- 230000001070 adhesive effect Effects 0.000 claims description 4
- 239000004020 conductor Substances 0.000 claims description 4
- 238000001816 cooling Methods 0.000 claims description 4
- 238000000502 dialysis Methods 0.000 claims description 4
- 238000010790 dilution Methods 0.000 claims description 4
- 239000012895 dilution Substances 0.000 claims description 4
- 239000006185 dispersion Substances 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 4
- 239000012362 glacial acetic acid Substances 0.000 claims description 4
- 238000007654 immersion Methods 0.000 claims description 4
- 230000035484 reaction time Effects 0.000 claims description 4
- 238000007789 sealing Methods 0.000 claims description 4
- 238000000926 separation method Methods 0.000 claims description 4
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 claims description 4
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 claims description 4
- 239000007787 solid Substances 0.000 claims description 4
- 239000002904 solvent Substances 0.000 claims description 4
- 239000004698 Polyethylene Substances 0.000 claims 1
- 230000004913 activation Effects 0.000 claims 1
- 238000000605 extraction Methods 0.000 claims 1
- 150000002466 imines Chemical class 0.000 claims 1
- 229920000573 polyethylene Polymers 0.000 claims 1
- 238000003756 stirring Methods 0.000 claims 1
- 230000004151 fermentation Effects 0.000 abstract description 17
- 238000000855 fermentation Methods 0.000 abstract description 15
- 238000004458 analytical method Methods 0.000 abstract description 11
- 239000004615 ingredient Substances 0.000 abstract description 3
- 238000005234 chemical deposition Methods 0.000 abstract description 2
- 230000000694 effects Effects 0.000 abstract description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 25
- 239000008103 glucose Substances 0.000 description 21
- 239000000047 product Substances 0.000 description 7
- 230000004044 response Effects 0.000 description 7
- 238000001514 detection method Methods 0.000 description 6
- 238000004445 quantitative analysis Methods 0.000 description 6
- 238000005259 measurement Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- NOWKCMXCCJGMRR-UHFFFAOYSA-N Aziridine Chemical compound C1CN1 NOWKCMXCCJGMRR-UHFFFAOYSA-N 0.000 description 3
- 229920002134 Carboxymethyl cellulose Polymers 0.000 description 3
- 150000001299 aldehydes Chemical class 0.000 description 3
- 235000010948 carboxy methyl cellulose Nutrition 0.000 description 3
- 239000008112 carboxymethyl-cellulose Substances 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- BHTJEPVNHUUIPV-UHFFFAOYSA-N pentanedial;hydrate Chemical compound O.O=CCCCC=O BHTJEPVNHUUIPV-UHFFFAOYSA-N 0.000 description 3
- 239000008363 phosphate buffer Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 239000002262 Schiff base Substances 0.000 description 2
- 150000004753 Schiff bases Chemical class 0.000 description 2
- 239000002585 base Substances 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 238000012512 characterization method Methods 0.000 description 2
- 230000005611 electricity Effects 0.000 description 2
- 230000005518 electrochemistry Effects 0.000 description 2
- 238000009655 industrial fermentation Methods 0.000 description 2
- 238000009776 industrial production Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000001681 protective effect Effects 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- 108010093096 Immobilized Enzymes Proteins 0.000 description 1
- 244000283207 Indigofera tinctoria Species 0.000 description 1
- 229910021607 Silver chloride Inorganic materials 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 238000010960 commercial process Methods 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 239000008121 dextrose Substances 0.000 description 1
- 238000000835 electrochemical detection Methods 0.000 description 1
- 238000004070 electrodeposition Methods 0.000 description 1
- 238000006056 electrooxidation reaction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000009144 enzymatic modification Effects 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 238000004451 qualitative analysis Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000011946 reduction process Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- HKZLPVFGJNLROG-UHFFFAOYSA-M silver monochloride Chemical compound [Cl-].[Ag+] HKZLPVFGJNLROG-UHFFFAOYSA-M 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
- G01N27/3277—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction being a redox reaction, e.g. detection by cyclic voltammetry
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/48—Systems using polarography, i.e. measuring changes in current under a slowly-varying voltage
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/902—Oxidoreductases (1.)
- G01N2333/904—Oxidoreductases (1.) acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Organic Chemistry (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- General Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Description
Claims (1)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201710329483.3A CN107238645B (zh) | 2017-05-11 | 2017-05-11 | 在线监测用葡萄糖氧化酶丝网印刷电极及其制备方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201710329483.3A CN107238645B (zh) | 2017-05-11 | 2017-05-11 | 在线监测用葡萄糖氧化酶丝网印刷电极及其制备方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN107238645A CN107238645A (zh) | 2017-10-10 |
CN107238645B true CN107238645B (zh) | 2019-04-30 |
Family
ID=59984323
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201710329483.3A Active CN107238645B (zh) | 2017-05-11 | 2017-05-11 | 在线监测用葡萄糖氧化酶丝网印刷电极及其制备方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN107238645B (zh) |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110146562A (zh) * | 2018-12-17 | 2019-08-20 | 浙江大学山东工业技术研究院 | 一种基于普鲁士蓝的无酶尿酸传感器及其制备方法 |
CN110146563A (zh) * | 2018-12-17 | 2019-08-20 | 浙江大学山东工业技术研究院 | 一种基于普鲁士蓝的无酶尿酸传感器 |
CN110643499A (zh) * | 2019-09-27 | 2020-01-03 | 南京工业大学 | 一种微生物发酵在线浓度分析仪的上下位机通讯方法 |
CN110804616B (zh) * | 2019-10-31 | 2021-06-22 | 山东省科学院生物研究所 | 一种密码子优化的葡萄糖氧化酶基因及其应用 |
CN111855774A (zh) * | 2020-05-19 | 2020-10-30 | 大连理工大学 | 一种基于普鲁士蓝的智能可穿戴酶基生物传感器及其制备方法和应用 |
CN112415062B (zh) * | 2020-11-27 | 2022-09-16 | 山东省科学院生物研究所 | 一种快速检测乙醇的电极***及使用电极***对乙醇检测的方法 |
CN114002292B (zh) * | 2021-11-02 | 2024-03-08 | 深圳清华大学研究院 | 一种唾液血糖校正装置及方法 |
CN114334223B (zh) * | 2021-11-22 | 2023-01-13 | 北京大学 | 一种导电浆料及应用 |
CN114002283A (zh) * | 2021-11-26 | 2022-02-01 | 南京伊桥科技有限公司 | 一种高选择性电化学硫化氢传感器及工作电极制备方法 |
CN115372448B (zh) * | 2022-10-26 | 2023-02-14 | 可孚医疗科技股份有限公司 | 一种葡萄糖检测卡及其制备方法 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4288552A (en) * | 1978-04-19 | 1981-09-08 | Novo Industri A/S | Immobilized intracellular enzymes |
CN101929977A (zh) * | 2010-08-31 | 2010-12-29 | 刘丹 | 酶生物电化学传感芯片及其制备和使用方法 |
CN103757042A (zh) * | 2014-01-15 | 2014-04-30 | 华南理工大学 | 基于自聚集短肽诱导的固定化腈水解酶及制备方法和应用 |
CN104833713A (zh) * | 2015-04-23 | 2015-08-12 | 南京工业大学 | 一种简易的酶生物传感器的制备方法 |
CN104962546A (zh) * | 2015-06-19 | 2015-10-07 | 浙江工业大学 | 一种含葡萄糖异构酶细胞的固定化方法 |
-
2017
- 2017-05-11 CN CN201710329483.3A patent/CN107238645B/zh active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4288552A (en) * | 1978-04-19 | 1981-09-08 | Novo Industri A/S | Immobilized intracellular enzymes |
CN101929977A (zh) * | 2010-08-31 | 2010-12-29 | 刘丹 | 酶生物电化学传感芯片及其制备和使用方法 |
CN103757042A (zh) * | 2014-01-15 | 2014-04-30 | 华南理工大学 | 基于自聚集短肽诱导的固定化腈水解酶及制备方法和应用 |
CN104833713A (zh) * | 2015-04-23 | 2015-08-12 | 南京工业大学 | 一种简易的酶生物传感器的制备方法 |
CN104962546A (zh) * | 2015-06-19 | 2015-10-07 | 浙江工业大学 | 一种含葡萄糖异构酶细胞的固定化方法 |
Non-Patent Citations (2)
Title |
---|
Ganapati D. Yadav等.Enzyme-catalysed optical resolution of mandelic acid via RS(∓)-methyl mandelate in non-aqueous media.《Biochemical Engineering Journal》.2004,第19卷第101-107页. * |
果汁原浆中葡萄糖含量测定的酶电极设计;綦翠华等;《食品科学》;20150731;第36卷(第14期);全文 * |
Also Published As
Publication number | Publication date |
---|---|
CN107238645A (zh) | 2017-10-10 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN107238645B (zh) | 在线监测用葡萄糖氧化酶丝网印刷电极及其制备方法 | |
CN2372689Y (zh) | 电流型生物传感器 | |
CN104833713B (zh) | 一种简易的酶生物传感器的制备方法 | |
Wen et al. | Application of a biosensor for monitoring of ethanol | |
CN112034026B (zh) | 一种用于检测亚硝酸盐的电化学传感器、制备方法及应用 | |
JPH01219661A (ja) | 酵素電極型のセンサーを用いての分析方法及び装置 | |
CN108776163B (zh) | 一种检测胆固醇的酶生物传感器及其制备方法与应用 | |
Liu et al. | A mediator‐free tyrosinase biosensor based on ZnO sol‐gel matrix | |
Mascini et al. | Glucose electrochemical probe with extended linearity for whole blood | |
CN105954346B (zh) | 三维纸基电化学比率计的制备方法 | |
CN107449816A (zh) | 全固态离子选择性电极、制备方法和生物传感器 | |
CN111537589A (zh) | 一种基于钴基金属有机框架无酶葡萄糖传感器检测葡萄糖的方法 | |
CN105572199A (zh) | 工作电极生物反应物及电极式测试条 | |
CN104359966A (zh) | 一种贵金属掺杂氧化锌纳米棒的葡萄糖传感器的制备方法 | |
CN111458516A (zh) | 一种检测细菌耐药性的电化学发光生物传感器及其制备方法 | |
CN113340958A (zh) | 一种高灵敏度槲皮素电化学传感器的工作电极及其应用 | |
CN105866226A (zh) | 一种葡萄糖氧化酶生物传感器的制备及使用方法 | |
EP0326421B1 (en) | An electroanalytical method | |
US8968825B1 (en) | Disposable palladium nanoparticle-modified graphite pencil electrode | |
CN113092553B (zh) | 一种无酶葡萄糖传感器检测葡萄糖的方法 | |
CN106568827A (zh) | 电化学检测体液5‑羟基吲哚乙酸的电极制备及检测方法 | |
CN112010359B (zh) | 一种NiO/C纳米复合电极材料及制备方法和应用 | |
CN111398378B (zh) | 用于检测葡萄糖的复合材料修饰电极的制备方法及电极 | |
CN104198553A (zh) | 一种生物传感器敏感膜及其制备方法与用途 | |
CN115097149A (zh) | 同时检测葡萄糖和胆固醇的方法、电极及电极制作方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right |
Effective date of registration: 20240307 Address after: E-E-101, Dezhou Zhongyuan Technology Innovation and Entrepreneurship Park, No. 6596 Dongfanghong East Road, Yuanqiao Town, Economic and Technological Development Zone, Dezhou City, Shandong Province, 253000 Patentee after: Zhigan Biotechnology (Shandong) Co.,Ltd. Country or region after: China Address before: 250014 No. 19, ASTRI Road, Lixia District, Shandong, Ji'nan Patentee before: BIOLOGY INSTITUTE OF SHANDONG ACADEMY OF SCIENCES Country or region before: China |
|
TR01 | Transfer of patent right |