CN107236827B - 猪传染性胃肠炎病毒检测试剂盒及检测方法 - Google Patents
猪传染性胃肠炎病毒检测试剂盒及检测方法 Download PDFInfo
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- CN107236827B CN107236827B CN201710675424.1A CN201710675424A CN107236827B CN 107236827 B CN107236827 B CN 107236827B CN 201710675424 A CN201710675424 A CN 201710675424A CN 107236827 B CN107236827 B CN 107236827B
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
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Abstract
Description
组A | 组B | 组C | |
正向***引物F3 | 0.2μmol | 0.3μmol | 0.1μmol |
反向***引物B3 | 0.1μmol | 0.2μmol | 0.1μmol |
交叉引物CPR | 1μmol | 1μmol | 0.9μmol |
正向探针DF5F | 0.4μmol | 0.4μmol | 0.3μmol |
反向探针DF5B | 0.8μmol | 0.8μmol | 0.7μmol |
MgSO4 | 2mmol | 3mmol | 1mmol |
dNTP溶液 | 0.4mmol | 0.5mmol | 0.3mmol |
Betaine | 1mol | 1mol | 0.9mol |
GspF酶8U/μL | 10U | 10U | 9U |
10×GspF Buffer | 2μL | 2μL | 2μL |
AMV | 0.2U | 0.2U | 0.1U |
25×RNA Secure | 0.8μL | 0.8μL | 0.8μL |
无菌双蒸水 | 补足到16μL | 补足到16μL | 补足到16μL |
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Families Citing this family (2)
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CN110438249A (zh) * | 2019-04-29 | 2019-11-12 | 浙江省疾病预防控制中心 | 一种弓形虫核酸恒温扩增检测试剂盒及应用方法 |
CN112725530A (zh) * | 2021-01-19 | 2021-04-30 | 中国计量大学 | 检测菜豆荚斑驳病毒的交叉引物扩增引物组、试剂盒及应用 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101358246A (zh) * | 2008-08-28 | 2009-02-04 | 中华人民共和国上海出入境检验检疫局 | 用于检测猪瘟病毒的lamp试剂盒及其制备方法 |
CN102373301A (zh) * | 2011-11-29 | 2012-03-14 | 重庆出入境检验检疫局检验检疫技术中心 | 猪传染性胃肠炎病毒等温扩增技术快速检测试剂盒及检测方法 |
CN104593525A (zh) * | 2015-02-04 | 2015-05-06 | 四川农业大学 | 猪传染性胃肠炎病毒的lamp检测试剂盒 |
KR20150069220A (ko) * | 2013-12-13 | 2015-06-23 | 한남대학교 산학협력단 | 실시간 중합효소 연쇄반응을 통한 전염성 위장염 바이러스의 정량 검출방법 |
CN105256058A (zh) * | 2015-11-20 | 2016-01-20 | 浙江省疾病预防控制中心 | 一种基孔肯雅热病毒的恒温扩增检测试剂盒及检测方法 |
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- 2017-08-09 CN CN201710675424.1A patent/CN107236827B/zh active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101358246A (zh) * | 2008-08-28 | 2009-02-04 | 中华人民共和国上海出入境检验检疫局 | 用于检测猪瘟病毒的lamp试剂盒及其制备方法 |
CN102373301A (zh) * | 2011-11-29 | 2012-03-14 | 重庆出入境检验检疫局检验检疫技术中心 | 猪传染性胃肠炎病毒等温扩增技术快速检测试剂盒及检测方法 |
KR20150069220A (ko) * | 2013-12-13 | 2015-06-23 | 한남대학교 산학협력단 | 실시간 중합효소 연쇄반응을 통한 전염성 위장염 바이러스의 정량 검출방법 |
CN104593525A (zh) * | 2015-02-04 | 2015-05-06 | 四川农业大学 | 猪传染性胃肠炎病毒的lamp检测试剂盒 |
CN105256058A (zh) * | 2015-11-20 | 2016-01-20 | 浙江省疾病预防控制中心 | 一种基孔肯雅热病毒的恒温扩增检测试剂盒及检测方法 |
Non-Patent Citations (2)
Title |
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"Portable and amplicon contamination prevention cartridges for DNA amplification coupled to lateral flow detection";Rui Wang et al.,;《Analytical Methods》;20151231;第7卷;第3692-3696页 * |
"猪圆环病毒2型交叉引物恒温扩增检测方法的建立";袁旦一等;《畜牧兽医学报》;20161231;第47卷(第9期);第1906、1908页 * |
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