CN107227204A - A kind of cichory root tobacco aromaticss and its preparation method and application - Google Patents
A kind of cichory root tobacco aromaticss and its preparation method and application Download PDFInfo
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- CN107227204A CN107227204A CN201710662174.8A CN201710662174A CN107227204A CN 107227204 A CN107227204 A CN 107227204A CN 201710662174 A CN201710662174 A CN 201710662174A CN 107227204 A CN107227204 A CN 107227204A
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/0003—Compounds of unspecified constitution defined by the chemical reaction for their preparation
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- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/20—Biochemical treatment
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- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B3/00—Preparing tobacco in the factory
- A24B3/12—Steaming, curing, or flavouring tobacco
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/15—Corynebacterium
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Abstract
The invention discloses a kind of cichory root tobacco aromaticss and its preparation method and application.After cichory root is crushed, the water of 10~20 times of amounts is added, fermentation medium is made in sterilizing under high-temperature and high-pressure conditions, and utilizes bacillus subtilis Bacillus subtilis（CGMCC NO.4091）It is fermented, filters, concentrate after the completion of fermentation, produce required tobacco aromaticss.Equably it is sprayed at when using as bed material on pipe tobacco.The present invention prepares essence spice for cigarette using fermentation of bacillus subtilis cichory root initiatively, active ingredient and its distinctive smell in cichory root with pipe tobacco after being combined, the quality of cigarette can be effectively improved, correct cigarette odor-absorbing, improve the aroma quality of cigarette, this method is suitable to popularization and application, has positive effect to promoting cigarette industry to develop in a healthy way.
Description
Technical field
The present invention relates to a kind of tobacco aromaticss, and in particular to a kind of cigarette prepared using cichory root as raw material is fragrant
Material.Meanwhile, the invention further relates to the preparation method and application of the volume tobacco aromaticss, belong to cigarette additive technical field.
Background technology
Essence and flavoring agent plays key effect to the taste of cigarette product.Current most spices is all by chemistry conjunction
Obtained into method, but not only cost is high, synthesis is difficult for the spices of chemical synthesis, and one can be caused to be fixed to environment and health
Ring.There is extraction cost height, the problems such as raw material is limited again in the spices directly extracted by natural animal-plant.Utilize microbial technique
The spices that fermenting plant material is prepared belongs to natural perfume material rank, and while product quality is improved, can save
Resource, reduces cost.
There is bacillus subtilis Van3 to be used for the research for improving quality of tobacco shreds at present, however, its fermentation time is up to 30 days,
And inhale bitter and puckery flavor, it is impossible to improve excitant well, perfume quantity is not enough.
Witloof is a kind of brushy herbaceos perennial, root meat, short and thick.In Yunnan Province of China, Sichuan（Chengdu）
And there is introducing and planting in Guangdong etc..Witloof is medical and edible dual purpose plant, and the modulated romaine lettuce of chicory leaf, root contains synanthrin and aromatic substance,
With clearing heat and detoxicating, inducing diuresis to remove edema, the effects such as stomach invigorating, coffee, beverage, tea, health products, food, Chinese medicine are currently widely used for
In.But fermentation extraction in the prior art, is carried out using microorganism to the nutriment and fragrance of witloof, and is used as flavouring essence for tobacco fragrant
There is presently no relevant report for the method that material is applied in cigarette.
The content of the invention
In view of the above-mentioned deficiencies in the prior art, it is an object of the present invention to provide a kind of cichory root tobacco aromaticss, applied
In cigarette, to lift the fragrance level and perfume quantity of cigarette product.
It is a further object of the present invention to provide a kind of method for preparing the cichory root tobacco aromaticss.
The present invention also aims to provide application of the cichory root tobacco aromaticss in production of cigarettes.
The purpose of the present invention is achieved by following technical proposals.
* unless otherwise indicated, the percentage employed in the present invention is mass percent.
A kind of cichory root tobacco aromaticss, it is characterised in that：After cichory root is crushed, bacillus subtilis is usedBacillus subtilisFermented, fermentation gains are required tobacco aromaticss；Described bacillus subtilisBacillus subtilisIt is stored in China Committee for Culture Collection of Microorganisms's common micro-organisms center；Preserving number is CGMCC
NO.4091；Preservation date：On 08 16th, 2010.Bacillus subtilisBacillus subtilisFrom flue-cured tobacco surface separation and
.
The preparation method of above-mentioned cichory root extractive from fermentative, it is characterised in that：Comprise the following steps：
（1）Bacillus subtillis CGMCC NO.4091 activation cultures are taken, bacillus subtilis activation bacterium solution is obtained；Actication of culture
Condition of culture is 30 DEG C~38 DEG C, 100~180r/min shaken cultivations, and incubation time is 6~24h；
（2）After cichory root is crushed, the distilled water of 10~20 times of amounts is added, sterilizing, which is made, treats fermented sample；
（3）The bacterium solution activated is inoculated in and treated in fermented sample, inoculum concentration is 0.5%-2.5%；Fermented and cultured bar after inoculation
Part is with activation culture condition；Incubation time is 6~24h；
（4）Zymotic fluid takes supernatant after filtering, centrifugation, is concentrated under reduced pressure into medicinal extract under the conditions of temperature is 45 DEG C~65 DEG C, i.e.,
Cichory root extractive from fermentative for needed for.
Application of the above-mentioned cichory root tobacco aromaticss in cigarette, it is characterised in that：In cigarette primary processing feeding stage, by institute
The cichory root tobacco aromaticss stated equably are sprayed on pipe tobacco, and addition is 5~20g/ case pipe tobaccos.
Compared with prior art, the present invention has advantages below：
1. the present invention utilizes bacillus subtilis initiativelyBacillus subtilisIt is fragrant that fermentation cichory root prepares flavouring essence for tobacco
Material, and be added in bed material form in cigarette.
2. being made after spices, the active ingredient and its distinctive fragrance in cichory root with pipe tobacco after being combined, Neng Goufeng
Rich cigarette perfume, lifts perfume quantity, further reduces blue foreign smell.
3. present invention process is simple, operation is easy, and cost is low, suitable for popularization and application, to promoting cigarette industry to develop in a healthy way
With positive effect.
Embodiment
It is clearly and completely described below in conjunction with the technical scheme in embodiment, it is clear that described embodiment is only
Only it is the example to a part of example of the invention, rather than whole.Based on the embodiment in the present invention, ordinary skill people
The every other embodiment that member is obtained under the premise of creative work is not paid, belongs to the scope of protection of the invention.Should
Work as understanding, the specific embodiments described herein are merely illustrative of the present invention, be not intended to limit the present invention.In embodiment not
Particular technique or condition person are indicated, is entered according to the technology or condition described by document in the art or according to product description
OK.Agents useful for same or the unreceipted production firm person of instrument, being can be by buying the conventional products obtained.LB used
(Luria-Bertani) culture medium：1% peptone, 1% sodium chloride, 0. 5% yeast extract.
Embodiment 1
The preparation method of the cichory root tobacco aromaticss of the present embodiment, comprises the following steps：
（1）Take Bacillus subtillis CGMCC NO.4091 to carry out activation culture in LB fluid nutrient mediums, obtain bacillus subtilis
Bacterium activates bacterium solution；Strain activation and culture condition is 37 DEG C, 120r/min shaken cultivations, and incubation time is 12h；
（2）The cichory root powder 100g after crushing is taken, the water of 15 times of amounts is added, sterilize 30min at 121 DEG C, is made and treats fermentation-like
Product；
（3）It is fermented using bacillus subtilis CGMCC NO.4091, by step（1）The middle bacterium solution inoculation activated
In treating in fermented sample, inoculation amount is 1%, and fermentation condition is：Rotating speed 120rmp, 37 DEG C of fermentation temperature, fermentation time 12h；
（4）Zymotic fluid takes supernatant after filtering, centrifugation, medicinal extract is concentrated under reduced pressure under the conditions of temperature is 50 DEG C, needed for being
Cichory root extractive from fermentative, preserve it is stand-by.
（5）With GC-MS to step（4）The aroma component of the tobacco aromaticss of gained is detected that testing result shows, the hair
The higher flavor matter of content mainly has furfural, palmitic acid, linoleic acid, ethyl palmitate, caproic acid, 2- acetyl group in ferment gains
Furans, 2- acetyl pyrroles, furfuryl alcohol, ethyl linoleate, 2- hydroxyl -4,6- dimethoxy-acetophenones, 3 methylvaleric acid, 2- methyl
Tetrahydrofuran-3-one, 2- cyclopentene -1,4- diketone, apiol, phenylacetaldehyde, 4- vinyl guaiacols, methyl hexadecanoate, pyrrole
Pyridine, vanillic aldehyde, eugenol, 2- acetylpyridines, pyrazine etc..
（6）By step（4）The tobacco aromaticss of gained are equably sprayed on pipe tobacco as bed material, and addition is 8g/ casees, and
Using the tobacco leaf without spices as control.The pipe tobacco for the treatment of group and control group is put into after climatic chamber inner equilibrium 24h, is made
Cigarette, sample and the equal random number of control, the group of smokeing panel test being made up of the expert that smokes panel test carry out sensory evaluating smoking.Perfuming, which is smoked panel test, tests knot
Fruit shows, the perfume additive harmony cigarette perfume, can significantly improve the fine and smooth alcohol of flue gas concentration, flue gas and, reduce excitant, cover miscellaneous
Gas, pleasant impression are clean, and assign cigarette a kind of pure and fresh special note.
Embodiment 2
The preparation method of the cichory root tobacco aromaticss of the present embodiment, comprises the following steps：
（1）Take Bacillus subtillis CGMCC NO.4091 to carry out activation culture in LB fluid nutrient mediums, obtain bacillus subtilis
Bacterium activates bacterium solution；Strain activation and culture condition is 37 DEG C, 120r/min shaken cultivations, and incubation time is 12h；
（2）The cichory root powder 100g after crushing is taken, the water of 20 times of amounts is added, sterilize 30min at 121 DEG C, is made and treats fermentation-like
Product；
（3）It is fermented using bacillus subtilis CGMCC NO.4091, by step（1）The middle bacterium solution inoculation activated
In treating in fermented sample, inoculation amount is 2%, and fermentation condition is：Rotating speed 120rmp, 37 DEG C of fermentation temperature, fermentation time 12h；
（4）Zymotic fluid takes supernatant after filtering, centrifugation, medicinal extract is concentrated under reduced pressure under the conditions of temperature is 50 DEG C, needed for being
Cichory root extractive from fermentative, preserve it is stand-by.
（5）To step（4）The aroma component of the tobacco aromaticss of gained is detected that testing result shows, obtained by the fermentation
In thing the higher flavor matter of content mainly have furfural, palmitic acid, linoleic acid, ethyl palmitate, caproic acid, 2- acetyl furans,
2- acetyl pyrroles, furfuryl alcohol, ethyl linoleate, 2- hydroxyl -4,6- dimethoxy-acetophenones, 3 methylvaleric acid, 2- methyl tetrahydrochysenes
Furans -3- ketone, 2- cyclopentene -1,4- diketone, apiol, phenylacetaldehyde, 4- vinyl guaiacols, methyl hexadecanoate, pyridine, perfume (or spice)
Lan Su, eugenol, 2- acetylpyridines, pyrazine etc..
（6）By step（4）The tobacco aromaticss of gained are equably sprayed on pipe tobacco as bed material, and addition is 10g/ casees,
And using the tobacco leaf without spices as control.The pipe tobacco for the treatment of group and control group is put into after climatic chamber inner equilibrium 24h, made
Into cigarette, sample and the equal random number of control, the group of smokeing panel test being made up of the expert that smokes panel test carry out sensory evaluating smoking.Perfuming is smoked panel test experiment
As a result show, the perfume additive harmony cigarette perfume, cover miscellaneous gas, reduce excitant, the soft exquisiteness of flue gas, pleasant impression is clean, promotes the production of body fluid.
Embodiment 3
The preparation method of the cichory root tobacco aromaticss of the present embodiment, comprises the following steps：
（1）Take Bacillus subtillis CGMCC NO.4091 to carry out activation culture in LB fluid nutrient mediums, obtain bacillus subtilis
Bacterium activates bacterium solution；Strain activation and culture condition is 33 DEG C, 160r/min shaken cultivations, and incubation time is 18h；
（2）The cichory root powder 100g after crushing is taken, the water of 15 times of amounts is added, sterilize 30min at 121 DEG C, is made and treats fermentation-like
Product；
（3）It is fermented using bacillus subtilis CGMCC NO.4091, by step（1）The middle bacterium solution inoculation activated
In treating in fermented sample, inoculation amount is 1%, and fermentation condition is：Rotating speed 160rmp, 33 DEG C of fermentation temperature, fermentation time 18h；
（4）Zymotic fluid takes supernatant after filtering, centrifugation, medicinal extract is concentrated under reduced pressure under the conditions of temperature is 50 DEG C, needed for being
Cichory root extractive from fermentative, preserve it is stand-by.
（5）To step（4）The aroma component of the tobacco aromaticss of gained is detected that testing result shows, obtained by the fermentation
In thing the higher flavor matter of content mainly have furfural, palmitic acid, linoleic acid, ethyl palmitate, caproic acid, 2- acetyl furans,
2- acetyl pyrroles, furfuryl alcohol, ethyl linoleate, 2- hydroxyl -4,6- dimethoxy-acetophenones, 3 methylvaleric acid, 2- methyl tetrahydrochysenes
Furans -3- ketone, 2- cyclopentene -1,4- diketone, apiol, phenylacetaldehyde, 4- vinyl guaiacols, methyl hexadecanoate, pyridine, perfume (or spice)
Lan Su, eugenol, 2- acetylpyridines, pyrazine etc..
（6）By step（4）The tobacco aromaticss of gained are equably sprayed on pipe tobacco as bed material, and addition is 12g/ casees,
And using the tobacco leaf without spices as control.The pipe tobacco for the treatment of group and control group is put into after climatic chamber inner equilibrium 24h, made
Into cigarette, sample and the equal random number of control, the group of smokeing panel test being made up of the expert that smokes panel test carry out sensory evaluating smoking.Perfuming is smoked panel test experiment
As a result show, the perfume additive harmony cigarette perfume, lift aroma quality, flue gas concentration covers miscellaneous gas, reduces excitant, and flue gas is soft
And exquisiteness, pleasant impression is totally comfortable.
Embodiment 4
The preparation method of the cichory root tobacco aromaticss of the present embodiment, comprises the following steps：
（1）Take Bacillus subtillis CGMCC NO.4091 to carry out activation culture in LB fluid nutrient mediums, obtain bacillus subtilis
Bacterium activates bacterium solution；Strain activation and culture condition is 33 DEG C, 160r/min shaken cultivations, and incubation time is 18h；
（2）The cichory root powder 100g after crushing is taken, the water of 20 times of amounts is added, sterilize 30min at 121 DEG C, is made and treats fermentation-like
Product；
（3）It is fermented using bacillus subtilis CGMCC NO.4091, by step（1）The middle bacterium solution inoculation activated
In treating in fermented sample, inoculation amount is 2%, and fermentation condition is：Rotating speed 160rmp, 33 DEG C of fermentation temperature, fermentation time 18h；
（4）Zymotic fluid takes supernatant after filtering, centrifugation, medicinal extract is concentrated under reduced pressure under the conditions of temperature is 50 DEG C, needed for being
Cichory root extractive from fermentative, preserve it is stand-by.
（5）To step（4）The aroma component of the tobacco aromaticss of gained is detected that testing result shows, obtained by the fermentation
In thing the higher flavor matter of content mainly have furfural, palmitic acid, linoleic acid, ethyl palmitate, caproic acid, 2- acetyl furans,
2- acetyl pyrroles, furfuryl alcohol, ethyl linoleate, 2- hydroxyl -4,6- dimethoxy-acetophenones, 3 methylvaleric acid, 2- methyl tetrahydrochysenes
Furans -3- ketone, 2- cyclopentene -1,4- diketone, apiol, phenylacetaldehyde, 4- vinyl guaiacols, methyl hexadecanoate, pyridine, perfume (or spice)
Lan Su, eugenol, 2- acetylpyridines, pyrazine etc..
（6）By step（4）The tobacco aromaticss of gained are equably sprayed on pipe tobacco as bed material, and addition is 15g/ casees,
And using the tobacco leaf without spices as control.The pipe tobacco for the treatment of group and control group is put into after climatic chamber inner equilibrium 24h, made
Into cigarette, sample and the equal random number of control, the group of smokeing panel test being made up of the expert that smokes panel test carry out sensory evaluating smoking.Perfuming is smoked panel test experiment
As a result show, the perfume additive harmony cigarette perfume, lifting aroma quality, flue gas concentration, the soft exquisiteness of flue gas cover miscellaneous gas, reduce
Excitant, pleasant impression is totally comfortable.
Embodiment 5
The preparation method of the cichory root tobacco aromaticss of the present embodiment, comprises the following steps：
（1）Take Bacillus subtillis CGMCC NO.4091 to carry out activation culture in LB fluid nutrient mediums, obtain bacillus subtilis
Bacterium activates bacterium solution；Strain activation and culture condition is 30 DEG C, 160r/min shaken cultivations, and incubation time is 24h；
（2）The cichory root powder 100g after crushing is taken, the water of 15 times of amounts is added, sterilize 30min at 121 DEG C, is made and treats fermentation-like
Product；
（3）It is fermented using bacillus subtilis CGMCC NO.4091, by step（1）The middle bacterium solution inoculation activated
In treating in fermented sample, inoculation amount is 1%, and fermentation condition is：Rotating speed 160rmp, 30 DEG C of fermentation temperature, fermentation time 24h；
（4）Zymotic fluid takes supernatant after filtering, centrifugation, medicinal extract is concentrated under reduced pressure under the conditions of temperature is 50 DEG C, needed for being
Cichory root extractive from fermentative, preserve it is stand-by.
（5）To step（4）The aroma component of the tobacco aromaticss of gained is detected that testing result shows, obtained by the fermentation
In thing the higher flavor matter of content mainly have furfural, palmitic acid, linoleic acid, ethyl palmitate, caproic acid, 2- acetyl furans,
2- acetyl pyrroles, furfuryl alcohol, ethyl linoleate, 2- hydroxyl -4,6- dimethoxy-acetophenones, 3 methylvaleric acid, 2- methyl tetrahydrochysenes
Furans -3- ketone, 2- cyclopentene -1,4- diketone, apiol, phenylacetaldehyde, 4- vinyl guaiacols, methyl hexadecanoate, pyridine, perfume (or spice)
Lan Su, eugenol, 2- acetylpyridines, pyrazine etc..
（6）By step（4）The tobacco aromaticss of gained are equably sprayed on pipe tobacco as bed material, and addition is 10g/ casees,
And using the tobacco leaf without spices as control.The pipe tobacco for the treatment of group and control group is put into after climatic chamber inner equilibrium 24h, made
Into cigarette, sample and the equal random number of control, the group of smokeing panel test being made up of the expert that smokes panel test carry out sensory evaluating smoking.Perfuming is smoked panel test experiment
As a result show, the perfume additive harmony cigarette perfume, flue gas concentration lifting is obvious, and fragrance exquisiteness is soft, suppress miscellaneous gas, pleasant impression is done
Only.
Embodiment 6
The preparation method of the cichory root tobacco aromaticss of the present embodiment, comprises the following steps：
（1）Take Bacillus subtillis CGMCC NO.4091 to carry out activation culture in LB fluid nutrient mediums, obtain bacillus subtilis
Bacterium activates bacterium solution；Strain activation and culture condition is 30 DEG C, 160r/min shaken cultivations, and incubation time is 24h；
（2）The cichory root powder 100g after crushing is taken, the water of 20 times of amounts is added, sterilize 30min at 121 DEG C, is made and treats fermentation-like
Product；
（3）It is fermented using bacillus subtilis CGMCC NO.4091, by step（1）The middle bacterium solution inoculation activated
In treating in fermented sample, inoculation amount is 2%, and fermentation condition is：Rotating speed 160rmp, 30 DEG C of fermentation temperature, fermentation time 24h；
（4）Zymotic fluid takes supernatant after filtering, centrifugation, medicinal extract is concentrated under reduced pressure under the conditions of temperature is 50 DEG C, needed for being
Cichory root extractive from fermentative, preserve it is stand-by.
（5）To step（4）The aroma component of the tobacco aromaticss of gained is detected that testing result shows, obtained by the fermentation
In thing the higher flavor matter of content mainly have furfural, palmitic acid, linoleic acid, ethyl palmitate, caproic acid, 2- acetyl furans,
2- acetyl pyrroles, furfuryl alcohol, ethyl linoleate, 2- hydroxyl -4,6- dimethoxy-acetophenones, 3 methylvaleric acid, 2- methyl tetrahydrochysenes
Furans -3- ketone, 2- cyclopentene -1,4- diketone, apiol, phenylacetaldehyde, 4- vinyl guaiacols, methyl hexadecanoate, pyridine, perfume (or spice)
Lan Su, eugenol, 2- acetylpyridines, pyrazine etc..
（6）By step（4）The tobacco aromaticss of gained are equably sprayed on pipe tobacco as bed material, and addition is 12g/ casees,
And using the tobacco leaf without spices as control.The pipe tobacco for the treatment of group and control group is put into after climatic chamber inner equilibrium 24h, made
Into cigarette, sample and the equal random number of control, the group of smokeing panel test being made up of the expert that smokes panel test carry out sensory evaluating smoking.Perfuming is smoked panel test experiment
As a result show, the perfume additive harmony cigarette perfume, fragrance exquisiteness is soft, cover miscellaneous gas, reduce excitant, increase sweet tea sense, pleasant impression
Totally, promote the production of body fluid.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
Any modifications, equivalent substitutions and improvements made within refreshing and principle etc., should be included in the scope of the protection.
Claims (3)
1. a kind of cichory root tobacco aromaticss, it is characterised in that：By cichory root fermentation of bacillus subtilis, fermentation gains pass through
It is required tobacco aromaticss after filter, concentration；Described bacillus subtilisBacillus subtilisIt is stored in Chinese micro- life
Thing culture presevation administration committee common micro-organisms center；Preserving number is CGMCC NO.4091；Preservation date：08 month 2010
16 days.
2. the preparation method of the cichory root tobacco aromaticss described in claim 1, it is characterised in that：Comprise the following steps：
（1）Bacillus subtillis CGMCC NO.4091 activation cultures are taken, bacillus subtilis activation bacterium solution is obtained；Actication of culture
Condition of culture is 30 DEG C~38 DEG C, 100~180r/min shaken cultivations, and incubation time is 6~24h；
（2）After cichory root is crushed, the water of 10~20 times of amounts is added, sterilizing, which is made, treats fermented sample；
（3）Step（1）The middle bacterium solution activated, which is inoculated in, to be treated in fermented sample, and inoculation amount is 0.5-2.5%, fermentation condition
For：Rotating speed 100-180rmp, 30-38 DEG C of fermentation temperature, fermentation time 6-24h；
（4）Zymotic fluid takes supernatant after filtering, centrifugation, is concentrated under reduced pressure into medicinal extract under the conditions of temperature is 45 DEG C~65 DEG C, i.e.,
Cichory root extractive from fermentative for needed for.
3. application of the cichory root tobacco aromaticss in cigarette described in claim 1, it is characterised in that：In cigarette primary processing charging rank
Section, described witloof tobacco aromaticss are equably sprayed on pipe tobacco, and addition is 5~20g/ case pipe tobaccos.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108018121A (en) * | 2018-01-05 | 2018-05-11 | 贵州省香瑞莱香料有限公司 | A kind of natural peppermint flavouring essence for tobacco of oil ben and preparation method thereof |
| CN109105953A (en) * | 2018-09-11 | 2019-01-01 | 云南中烟工业有限责任公司 | A kind of soft quasi- carbonization ferment particulate of sorghum pueraria lobata Bai Xuecha, preparation method and the usage |
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Cited By (8)
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|---|---|---|---|---|
| CN108018121A (en) * | 2018-01-05 | 2018-05-11 | 贵州省香瑞莱香料有限公司 | A kind of natural peppermint flavouring essence for tobacco of oil ben and preparation method thereof |
| CN109105953A (en) * | 2018-09-11 | 2019-01-01 | 云南中烟工业有限责任公司 | A kind of soft quasi- carbonization ferment particulate of sorghum pueraria lobata Bai Xuecha, preparation method and the usage |
| CN109222230A (en) * | 2018-09-11 | 2019-01-18 | 云南中烟工业有限责任公司 | A kind of soft quasi- carbonization ferment particulate of corn Radix Glycyrrhizae blood-snow tea, preparation method and the usage |
| CN109222230B (en) * | 2018-09-11 | 2021-04-06 | 云南中烟工业有限责任公司 | Corn, liquorice, black snow tea soft and quasi-carbonized fermentation particles and preparation method and application thereof |
| CN110292203A (en) * | 2018-10-18 | 2019-10-01 | 云南中烟工业有限责任公司 | A kind of soft quasi- carbonization ferment particulate of plant, preparation method and the usage |
| CN110292203B (en) * | 2018-10-18 | 2021-08-03 | 云南中烟工业有限责任公司 | Plant soft-simulated carbonized fermentation particles, and preparation method and application thereof |
| CN114041621A (en) * | 2021-11-22 | 2022-02-15 | 红云红河烟草(集团)有限责任公司 | Method for preparing tobacco flavor sugar material by utilizing industrial tobacco waste |
| CN116676138A (en) * | 2023-07-25 | 2023-09-01 | 内蒙古昆明卷烟有限责任公司 | Oncidium essential oil for cigarettes and preparation method and application thereof |
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