CN104450191A - Lactobacillus-fermented tea leaf extract for cigarettes and preparation method of lactobacillus-fermented tea leaf extract for cigarettes - Google Patents
Lactobacillus-fermented tea leaf extract for cigarettes and preparation method of lactobacillus-fermented tea leaf extract for cigarettes Download PDFInfo
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- CN104450191A CN104450191A CN201410733930.8A CN201410733930A CN104450191A CN 104450191 A CN104450191 A CN 104450191A CN 201410733930 A CN201410733930 A CN 201410733930A CN 104450191 A CN104450191 A CN 104450191A
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- 235000019504 cigarettes Nutrition 0.000 title claims abstract description 40
- 229940092665 tea leaf extract Drugs 0.000 title claims abstract description 33
- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- 238000001914 filtration Methods 0.000 claims abstract description 17
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 9
- 229930006000 Sucrose Natural products 0.000 claims abstract description 9
- 239000005720 sucrose Substances 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 9
- 239000007788 liquid Substances 0.000 claims abstract description 6
- 238000010992 reflux Methods 0.000 claims abstract description 6
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 26
- 244000269722 Thea sinensis Species 0.000 claims description 16
- 235000014655 lactic acid Nutrition 0.000 claims description 13
- 241000894006 Bacteria Species 0.000 claims description 12
- 239000000706 filtrate Substances 0.000 claims description 12
- 235000006468 Thea sinensis Nutrition 0.000 claims description 8
- 239000000047 product Substances 0.000 claims description 7
- 235000020279 black tea Nutrition 0.000 claims description 5
- 235000009569 green tea Nutrition 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 3
- 235000020333 oolong tea Nutrition 0.000 claims description 3
- 239000012141 concentrate Substances 0.000 claims description 2
- 239000000796 flavoring agent Substances 0.000 abstract description 8
- 235000019634 flavors Nutrition 0.000 abstract description 8
- 239000003205 fragrance Substances 0.000 abstract description 8
- 235000009508 confectionery Nutrition 0.000 abstract description 7
- 238000001816 cooling Methods 0.000 abstract description 4
- 238000010438 heat treatment Methods 0.000 abstract description 4
- 238000002156 mixing Methods 0.000 abstract description 2
- 241000186660 Lactobacillus Species 0.000 abstract 2
- 229940039696 lactobacillus Drugs 0.000 abstract 2
- 241001122767 Theaceae Species 0.000 abstract 1
- 230000003247 decreasing effect Effects 0.000 abstract 1
- 230000007794 irritation Effects 0.000 abstract 1
- 241000208125 Nicotiana Species 0.000 description 17
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 17
- 238000000855 fermentation Methods 0.000 description 14
- 230000004151 fermentation Effects 0.000 description 14
- 238000000034 method Methods 0.000 description 12
- 235000013616 tea Nutrition 0.000 description 7
- 239000004744 fabric Substances 0.000 description 6
- 239000012535 impurity Substances 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 238000000967 suction filtration Methods 0.000 description 6
- 235000019633 pungent taste Nutrition 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- UGFAIRIUMAVXCW-UHFFFAOYSA-N Carbon monoxide Chemical compound [O+]#[C-] UGFAIRIUMAVXCW-UHFFFAOYSA-N 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 239000003546 flue gas Substances 0.000 description 3
- 239000002054 inoculum Substances 0.000 description 3
- 150000008442 polyphenolic compounds Chemical class 0.000 description 3
- 235000013824 polyphenols Nutrition 0.000 description 3
- 238000004062 sedimentation Methods 0.000 description 3
- 230000000391 smoking effect Effects 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- 235000019640 taste Nutrition 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- GLZPCOQZEFWAFX-UHFFFAOYSA-N Geraniol Chemical compound CC(C)=CCCC(C)=CCO GLZPCOQZEFWAFX-UHFFFAOYSA-N 0.000 description 2
- 150000001299 aldehydes Chemical class 0.000 description 2
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 description 2
- 150000002576 ketones Chemical class 0.000 description 2
- 239000004310 lactic acid Substances 0.000 description 2
- 229920002521 macromolecule Polymers 0.000 description 2
- 235000013599 spices Nutrition 0.000 description 2
- 235000012976 tarts Nutrition 0.000 description 2
- PFTAWBLQPZVEMU-DZGCQCFKSA-N (+)-catechin Chemical compound C1([C@H]2OC3=CC(O)=CC(O)=C3C[C@@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-DZGCQCFKSA-N 0.000 description 1
- 241000722824 Ardisia crenata Species 0.000 description 1
- WVDDGKGOMKODPV-UHFFFAOYSA-N Benzyl alcohol Chemical compound OCC1=CC=CC=C1 WVDDGKGOMKODPV-UHFFFAOYSA-N 0.000 description 1
- 239000004215 Carbon black (E152) Substances 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 239000005792 Geraniol Substances 0.000 description 1
- GLZPCOQZEFWAFX-YFHOEESVSA-N Geraniol Natural products CC(C)=CCC\C(C)=C/CO GLZPCOQZEFWAFX-YFHOEESVSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 125000003118 aryl group Chemical group 0.000 description 1
- 229960004217 benzyl alcohol Drugs 0.000 description 1
- 235000019445 benzyl alcohol Nutrition 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- ADRVNXBAWSRFAJ-UHFFFAOYSA-N catechin Natural products OC1Cc2cc(O)cc(O)c2OC1c3ccc(O)c(O)c3 ADRVNXBAWSRFAJ-UHFFFAOYSA-N 0.000 description 1
- 235000005487 catechin Nutrition 0.000 description 1
- 229950001002 cianidanol Drugs 0.000 description 1
- -1 cis-3-enol Chemical compound 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 229940113087 geraniol Drugs 0.000 description 1
- 229930195733 hydrocarbon Natural products 0.000 description 1
- 150000002430 hydrocarbons Chemical class 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 150000002596 lactones Chemical class 0.000 description 1
- CDOSHBSSFJOMGT-UHFFFAOYSA-N linalool Chemical compound CC(C)=CCCC(C)(O)C=C CDOSHBSSFJOMGT-UHFFFAOYSA-N 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 150000002927 oxygen compounds Chemical class 0.000 description 1
- 239000002304 perfume Substances 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 235000019260 propionic acid Nutrition 0.000 description 1
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 1
- 230000001953 sensory effect Effects 0.000 description 1
- 210000000697 sensory organ Anatomy 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 235000019505 tobacco product Nutrition 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/0026—Essential oils; Perfumes compounds containing an alicyclic ring not condensed with another ring
- C11B9/003—Essential oils; Perfumes compounds containing an alicyclic ring not condensed with another ring the ring containing less than six carbon atoms
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B3/00—Preparing tobacco in the factory
- A24B3/12—Steaming, curing, or flavouring tobacco
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Manufacture Of Tobacco Products (AREA)
- Tea And Coffee (AREA)
Abstract
The invention provides a preparation method of a lactobacillus-fermented tea leaf extract for cigarettes. The preparation method comprises the following steps: (1) mixing tea leaves with water at a material-liquid ratio of 1 to (6-10), carrying out heating reflux for 1-3 hours, cooling, and filtering to obtain a tea leaf extract; (2) fermenting the tea leaf extract obtained in the step (1) with lactobacillus and sucrose at 28-37 DEG C for 3 days to obtain a fermented product, wherein the volume of the lactobacillus is equal to 1.0%-3.0% of that of the tea leaf extract, and the volume of the added sucrose is 6% of that of the tea leaf extract; and (3) filtering the fermented product, and concentrating the filtered fermented product so as to obtain the lactobacillus-fermented tea leaf extract for the cigarettes. By adding the lactobacillus-fermented tea leaf extract for the cigarettes, the cigarettes have relatively rich flavors and relatively obvious sweetness, furthermore, the fresh and sweet fragrances are increased, the elegancy is relatively high, and the irritation is decreased; the preparation method has the very strong practicability.
Description
Technical field
The present invention relates to a kind of preparation method of tobacco aromatics using, specifically a kind of preparation method of lactobacillus-fermented type cigarette tealeaves medicinal extract.
Background technology
In tobacco product, fragrance and the suction taste of tobacco are vital, interpolation spices is the key link in cigarette process, is also to improve one of product style and the effective means improving cigarette flavor quality, can provide some fragrance to human consumer and inhale the cigarette products of taste satisfaction.At present, the tobacco aromatics using added in China tobacco uses conventional methods preparation mostly, namely form with natural extract monomer and synthon mixing preparation, although tobacco aromatics using market operation obtained is like this simple, but there is fragrance dullness, not enough to tobacco improvement, and improve the shortcomings such as cigarette smoking quality DeGrain.
Containing abundant aroma substance in tealeaves, up to now, the tea aroma material of isolation identification about has 700 kinds, and the composition of tea aroma material comprises hydrocarbon polymer (14.22%), alcohols (12.76%), aldehydes (10.30%), ketone (15.35%), ester class and lactone (12.44%), containing N compound (13.41%), acids, phenols, assorted oxygen compound, sulfocompound class etc.But its main component is only tens of kinds, as Geraniol, cis-3-enol, phantol and oxide compound, phenylcarbinol etc.It is total that they have plenty of black tea, green tea, fresh leaf, have plenty of unique respectively separately, has plenty of to synthesize in fresh leaf process of growth, and what have is then formed in Tea Processing process.Generally speaking, in tea fresh leaves, the aroma substance kind contained is less, and about more than 80 plant; More than 260 are had to plant in green tea; Black tea then has kind more than 400.
Natural tobacco aromatics using used in the prior art mostly is the direct extract of spice berry, and is that the method for prepared using microbial fermentation technology production characteristic tobacco aromatics using has no report with tealeaves.
Summary of the invention
The object of the invention is the preparation method that a kind of lactobacillus-fermented type cigarette tealeaves medicinal extract is provided according to the deficiencies in the prior art, the cigarette tealeaves medicinal extract prepared by the inventive method is added in cigarette has pleasant sour-sweet fragrance, reduce pungency, and coordinate fragrance.
The present invention is achieved through the following technical solutions: a kind of preparation method of lactobacillus-fermented type cigarette tealeaves medicinal extract, its step comprises: tealeaves mixes according to solid-liquid ratio 1:6 ~ 10 with water by (1), reflux is after 1 ~ 3 hour, and cold filtration obtains tea leaf extract; (2) fermented together with 28 ~ 37 DEG C 3 days with milk-acid bacteria, sucrose by the tea leaf extract in step (1), the volume of described milk-acid bacteria is 1.0 ~ 3.0% of tea leaf extract, and the volume adding sucrose is 6% of tea leaf extract; (3) concentrate after fermented product being filtered and obtain described lactobacillus-fermented type cigarette tealeaves medicinal extract.
Preferably, concentrated in described step (3) refers to, first by filtrate concentrating under reduced pressure at 80 DEG C, then the massfraction adding 3 ~ 5 times of volumes is the spirituous solution back dissolving of 95%, is then cooled to-20 DEG C and carries out settlement treatment 18 ~ 24h; Solution after settlement treatment is carried out coarse filtration, the filtrate obtained again is filtered under 0.45 μm of aperture, it is 1.2000 ~ 1.5000 ± 0.010 that the filtrate obtaining secondary filtration is evaporated to density at 65 DEG C, is described lactobacillus-fermented type cigarette tealeaves medicinal extract.
Preferably, the pressure of described concentrating under reduced pressure is-0.01 ~-0.1Mpa.
Preferably, described tealeaves is one or more mixtures in black tea, green tea or oolong tea.
Utilize milk-acid bacteria fermentable saccharide, produce the soft lactic acid of tart flavour and multiple enzyme, eliminate the negative impact of some macromolecular complex confrontation cigarette.In addition, also the organic acid such as acetic acid, propionic acid can be produced in fermenting process.They are while imparting goods are with tart flavour, also can with the matter interaction such as alcohol, aldehyde, ketone that produces in lactic fermentation process, form multiple new taste compound, improve goods local flavor, the sour Studies of The Aromatic Substances of generation can play the effect increasing delicate fragrance note and elegant sense in cigarette.Containing more tea polyphenols material in tealeaves, catechin wherein adds in tobacco leaf can cause detrimentally affect to cigarette quality as tobacco extract, and milk-acid bacteria is exactly to tea-polyphenol material sensitive, its content can be reduced by fermenting process, play the effect improving cigarette quality.
The present invention's lactobacillus-fermented process tea leaf extract, macromolecular substance and tea polyphenols substances content in tealeaves, reduce cigarette pungency and it be converted into the sour odor type materials such as lactic acid, becoming unfavorable into favourable.Fermentation type tobacco tealeaves medicinal extract prepared by the present invention has pleasant sour-sweet fragrance.Add in cigarette by fermentation type tobacco tealeaves medicinal extract, have and enrich cigarette perfume (or spice), increase flue gas concentration, sweet flue gas, increases the effects such as fresh and sweet note, and flue gas also more has elegant sense simultaneously.With not with compared with the tealeaves medicinal extract of milk-acid bacteria process, this medicinal extract can make cigarette flavor abundanter, and sweet sense is more obvious, and increases fresh and sweet note, and elegant sense is better, and pungency reduces.Its major cause is to cause the large macromolecular substance of cigarette pungency and tea polyphenols material to be utilized by milk-acid bacteria in tealeaves medicinal extract, make cigarette flavor abundanter, thus improve the quality of tealeaves medicinal extract, achieve the application of tealeaves medicinal extract in cigarette, there is very strong practicality.
Embodiment
By the following specific examples further illustrate the invention:
Embodiment 1
1) tea leaf extract: get black tea leaf, adds distilled water, and by tealeaves and water solid-liquid ratio 1:8, heating and refluxing extraction 2h, after then extracting solution being left standstill cooling, filter-cloth filtering obtains tea leaf extract;
2) lactobacillus-fermented: take tea leaf extract as substrate, under sterile closed state, it is fermented, the condition of fermentation is: the volume that sucrose adds is 6% of tea leaf extract volume, the inoculum size volume of milk-acid bacteria is 1.0% of tea leaf extract, leavening temperature is 28 DEG C, and fermentation time is 3 days;
3) aftertreatment of fermented product: filter and remove after large granular impurity, 80 DEG C under-0.01 ~-0.1Mpa first time concentrated filtrate, the massfraction adding 4 times of volumes is the spirituous solution back dissolving of 95% ,-20 DEG C of cooling-sedimentation process 18h; Use filter-cloth filtering large granular impurity, then use the millipore filtration suction filtration in 0.45 μm of aperture, the clear filtrate obtained by suction filtration second time concentrating under reduced pressure is collected, and temperature 65 DEG C, being concentrated into medicinal extract density is 1.2000-1.5000 ± 0.010 (20 DEG C) g/cm
3obtain fermentation type tobacco tealeaves medicinal extract 1.
Embodiment 2
1) tea leaf extract: get green tea, adds distilled water, and by tealeaves and water solid-liquid ratio 1:6, heating and refluxing extraction 1h, after then extracting solution being left standstill cooling, filter-cloth filtering obtains tea leaf extract;
2) lactobacillus-fermented: take tea leaf extract as substrate, under sterile closed state, it is fermented, the condition of fermentation is: the volume that sucrose adds is 6% of tea leaf extract volume, the inoculum size volume of milk-acid bacteria is 4.0% of tea leaf extract, leavening temperature is 37 DEG C, and fermentation time is 2 days;
3) aftertreatment of fermented product: filter and remove after large granular impurity, 80 DEG C under-0.01 ~-0.1Mpa first time concentrated filtrate, the massfraction adding 3 times of volumes is the spirituous solution back dissolving of 95% ,-20 DEG C of cooling-sedimentation process 20h; Use filter-cloth filtering large granular impurity, then use the millipore filtration suction filtration in 0.45 μm of aperture, the clear filtrate obtained by suction filtration second time concentrating under reduced pressure is collected, and temperature 65 DEG C, being concentrated into medicinal extract density is 1.2000-1.5000 ± 0.010 (20 DEG C) g/cm
3obtain fermentation type tobacco tealeaves medicinal extract 2.
Embodiment 3
1) tea leaf extract: get frozen fresh oolong tea, adds distilled water, and by tealeaves and water solid-liquid ratio 1:10, heating and refluxing extraction 3h, after then extracting solution being left standstill cooling, filter-cloth filtering obtains tea leaf extract;
2) lactobacillus-fermented: take tea leaf extract as substrate, under sterile closed state, it is fermented, the condition of fermentation is: the volume that sucrose adds is 6% of tea leaf extract volume, the inoculum size volume of milk-acid bacteria is 2.0% of tea leaf extract, leavening temperature is 30 DEG C, and fermentation time is 1 day;
3) aftertreatment of fermented product: filter and remove after large granular impurity, 80 DEG C under-0.01 ~-0.1Mpa first time concentrated filtrate, the massfraction adding 5 times of volumes is the spirituous solution back dissolving of 95% ,-20 DEG C of cooling-sedimentation process 24h; Use filter-cloth filtering large granular impurity, then use the millipore filtration suction filtration in 0.45 μm of aperture, the clear filtrate obtained by suction filtration second time concentrating under reduced pressure is collected, and temperature 65 DEG C, being concentrated into medicinal extract density is 1.2000-1.5000 ± 0.010 (20 DEG C) g/cm
3obtain fermentation type tobacco tealeaves medicinal extract 3.
Added in proportion in pipe tobacco by golden flower bacterium fermentation type tobacco tealeaves medicinal extract 1 in above-described embodiment 1, its consumption in cigarette is 0.01 ~ 0.5% for its application quantity, smokes panel test to the sense organ of cigarette.Smoking result is as shown in table 1:
Table 1 adds the cigarette sensory evaluation effect of tealeaves medicinal extract
According to smoking result, add lactobacillus-fermented type cigarette tealeaves medicinal extract of the present invention and cigarette flavor can be made abundanter, sweet sense is more obvious, and increases fresh and sweet note, and elegant sense is better, and pungency reduces.
Claims (4)
1. a lactobacillus-fermented type cigarette preparation method for tealeaves medicinal extract, its step comprises: tealeaves mixes according to solid-liquid ratio 1:6 ~ 10 with water by (1), and reflux is after 1 ~ 3 hour, and cold filtration obtains tea leaf extract; (2) fermented together with 28 ~ 37 DEG C 3 days with milk-acid bacteria, sucrose by the tea leaf extract in step (1), the volume of described milk-acid bacteria is 1.0 ~ 3.0% of tea leaf extract, and the volume adding sucrose is 6% of tea leaf extract; (3) concentrate after fermented product being filtered and obtain described lactobacillus-fermented type cigarette tealeaves medicinal extract.
2. the lactobacillus-fermented type cigarette preparation method of tealeaves medicinal extract, it is characterized in that: concentrated in described step (3) refers to, first by filtrate at 80 DEG C after concentrating under reduced pressure, the massfraction adding 3 ~ 5 times of volumes is again the spirituous solution back dissolving of 95%, is then cooled to-20 DEG C and carries out settlement treatment 18 ~ 24h; Solution after settlement treatment is carried out coarse filtration, the filtrate obtained again is filtered under 0.45 μm of aperture, it is 1.2000 ~ 1.5000 ± 0.010 that the filtrate obtaining secondary filtration is evaporated to density at 65 DEG C, is described lactobacillus-fermented type cigarette tealeaves medicinal extract.
3. the lactobacillus-fermented type cigarette preparation method of tealeaves medicinal extract according to claim 2, is characterized in that: the pressure of described concentrating under reduced pressure is-0.01 ~-0.1Mpa.
4. the lactobacillus-fermented type cigarette preparation method of tealeaves medicinal extract according to claim 1, is characterized in that: described tealeaves is one or more mixtures in black tea, green tea or oolong tea.
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Cited By (6)
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| CN104946388A (en) * | 2015-04-29 | 2015-09-30 | 湖北中烟工业有限责任公司 | Fermented black tea spice for cigarettes and preparation method of fermented black tea spice |
| CN105779307A (en) * | 2016-04-18 | 2016-07-20 | 云南中烟工业有限责任公司 | Trichoderma aureoviride strain and method for preparing Pu'er Tea tobacco flavor |
| CN106136303A (en) * | 2016-08-31 | 2016-11-23 | 湖北中烟工业有限责任公司 | A kind of preparation method and applications of Fructus Gardeniae cigarette extract |
| CN106497906A (en) * | 2016-10-13 | 2017-03-15 | 湖北中烟工业有限责任公司 | A kind of preparation method of cigarette chamomile extract |
| CN106957873A (en) * | 2016-10-13 | 2017-07-18 | 武汉黄鹤楼香精香料有限公司 | A kind of fixing lactic acid bacteria prepares method of the cigarette with Acacia extract |
| CN108192724A (en) * | 2018-01-10 | 2018-06-22 | 湖北中烟工业有限责任公司 | A kind of preparation method and applications of fermentation type tobacco cherry extract |
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| CN106136303A (en) * | 2016-08-31 | 2016-11-23 | 湖北中烟工业有限责任公司 | A kind of preparation method and applications of Fructus Gardeniae cigarette extract |
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