CN106551422A - Preparation method and application of the cigarette with Semen Ziziphi Spinosae extractum - Google Patents
Preparation method and application of the cigarette with Semen Ziziphi Spinosae extractum Download PDFInfo
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- CN106551422A CN106551422A CN201611082854.4A CN201611082854A CN106551422A CN 106551422 A CN106551422 A CN 106551422A CN 201611082854 A CN201611082854 A CN 201611082854A CN 106551422 A CN106551422 A CN 106551422A
- Authority
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- China
- Prior art keywords
- ziziphi spinosae
- semen ziziphi
- cigarette
- extractum
- extracting solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- 210000000582 semen Anatomy 0.000 title claims abstract description 57
- 235000019504 cigarettes Nutrition 0.000 title claims abstract description 45
- 238000002360 preparation method Methods 0.000 title claims abstract description 18
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims abstract description 114
- 241000894006 Bacteria Species 0.000 claims abstract description 44
- 238000011218 seed culture Methods 0.000 claims abstract description 43
- 239000012531 culture fluid Substances 0.000 claims abstract description 33
- 235000013305 food Nutrition 0.000 claims abstract description 29
- 241000193755 Bacillus cereus Species 0.000 claims abstract description 28
- 241000196324 Embryophyta Species 0.000 claims abstract description 27
- 239000007788 liquid Substances 0.000 claims abstract description 26
- 238000000855 fermentation Methods 0.000 claims abstract description 17
- 230000004151 fermentation Effects 0.000 claims abstract description 17
- 239000000706 filtrate Substances 0.000 claims abstract description 13
- 240000001080 Grifola frondosa Species 0.000 claims abstract description 11
- 235000007710 Grifola frondosa Nutrition 0.000 claims abstract description 11
- 239000000203 mixture Substances 0.000 claims abstract description 10
- 244000183278 Nephelium litchi Species 0.000 claims abstract description 8
- 239000012530 fluid Substances 0.000 claims abstract description 8
- 238000001914 filtration Methods 0.000 claims abstract description 6
- 239000002244 precipitate Substances 0.000 claims abstract description 6
- 239000002904 solvent Substances 0.000 claims abstract description 6
- 239000001963 growth medium Substances 0.000 claims description 24
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 18
- 244000061176 Nicotiana tabacum Species 0.000 claims description 16
- 235000002637 Nicotiana tabacum Nutrition 0.000 claims description 16
- 239000002609 medium Substances 0.000 claims description 16
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 14
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 12
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 claims description 11
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 claims description 11
- 239000008103 glucose Substances 0.000 claims description 11
- 229920001817 Agar Polymers 0.000 claims description 10
- 239000001888 Peptone Substances 0.000 claims description 10
- 108010080698 Peptones Proteins 0.000 claims description 10
- 241000588746 Raoultella planticola Species 0.000 claims description 10
- 239000008272 agar Substances 0.000 claims description 10
- 238000009630 liquid culture Methods 0.000 claims description 10
- 235000019319 peptone Nutrition 0.000 claims description 10
- 238000002156 mixing Methods 0.000 claims description 9
- 239000011780 sodium chloride Substances 0.000 claims description 9
- 241000563903 Bacillus velezensis Species 0.000 claims description 7
- UGFAIRIUMAVXCW-UHFFFAOYSA-N Carbon monoxide Chemical compound [O+]#[C-] UGFAIRIUMAVXCW-UHFFFAOYSA-N 0.000 claims description 7
- 239000003546 flue gas Substances 0.000 claims description 7
- 239000003205 fragrance Substances 0.000 claims description 7
- 244000061456 Solanum tuberosum Species 0.000 claims description 6
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 6
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 6
- 229930006000 Sucrose Natural products 0.000 claims description 6
- 239000005720 sucrose Substances 0.000 claims description 6
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 5
- 239000002253 acid Substances 0.000 claims description 5
- 238000007654 immersion Methods 0.000 claims description 5
- 238000010992 reflux Methods 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 235000014121 butter Nutrition 0.000 claims description 4
- 235000009508 confectionery Nutrition 0.000 claims description 4
- 239000001965 potato dextrose agar Substances 0.000 claims description 4
- 238000000605 extraction Methods 0.000 claims description 3
- 230000006872 improvement Effects 0.000 claims description 2
- 230000003213 activating effect Effects 0.000 claims 1
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 abstract description 6
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract description 6
- 102000004190 Enzymes Human genes 0.000 abstract description 6
- 108090000790 Enzymes Proteins 0.000 abstract description 6
- 229910052799 carbon Inorganic materials 0.000 abstract description 6
- 239000000463 material Substances 0.000 abstract description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 4
- 230000008901 benefit Effects 0.000 abstract description 4
- 238000000034 method Methods 0.000 abstract description 4
- 230000000813 microbial effect Effects 0.000 abstract description 4
- KSEBMYQBYZTDHS-HWKANZROSA-M (E)-Ferulic acid Natural products COC1=CC(\C=C\C([O-])=O)=CC=C1O KSEBMYQBYZTDHS-HWKANZROSA-M 0.000 abstract description 3
- IVOMOUWHDPKRLL-KQYNXXCUSA-N Cyclic adenosine monophosphate Chemical compound C([C@H]1O2)OP(O)(=O)O[C@H]1[C@@H](O)[C@@H]2N1C(N=CN=C2N)=C2N=C1 IVOMOUWHDPKRLL-KQYNXXCUSA-N 0.000 abstract description 3
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 abstract description 3
- 229930003268 Vitamin C Natural products 0.000 abstract description 3
- 229930013930 alkaloid Natural products 0.000 abstract description 3
- 150000003797 alkaloid derivatives Chemical class 0.000 abstract description 3
- 235000001014 amino acid Nutrition 0.000 abstract description 3
- 150000001413 amino acids Chemical class 0.000 abstract description 3
- KSEBMYQBYZTDHS-HWKANZROSA-N ferulic acid Chemical compound COC1=CC(\C=C\C(O)=O)=CC=C1O KSEBMYQBYZTDHS-HWKANZROSA-N 0.000 abstract description 3
- 229940114124 ferulic acid Drugs 0.000 abstract description 3
- KSEBMYQBYZTDHS-UHFFFAOYSA-N ferulic acid Natural products COC1=CC(C=CC(O)=O)=CC=C1O KSEBMYQBYZTDHS-UHFFFAOYSA-N 0.000 abstract description 3
- 235000001785 ferulic acid Nutrition 0.000 abstract description 3
- 229930003935 flavonoid Natural products 0.000 abstract description 3
- 150000002215 flavonoids Chemical class 0.000 abstract description 3
- 235000017173 flavonoids Nutrition 0.000 abstract description 3
- 235000002378 plant sterols Nutrition 0.000 abstract description 3
- QURCVMIEKCOAJU-UHFFFAOYSA-N trans-isoferulic acid Natural products COC1=CC=C(C=CC(O)=O)C=C1O QURCVMIEKCOAJU-UHFFFAOYSA-N 0.000 abstract description 3
- -1 triterpeness Chemical class 0.000 abstract description 3
- 235000019154 vitamin C Nutrition 0.000 abstract description 3
- 239000011718 vitamin C Substances 0.000 abstract description 3
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 2
- 239000000758 substrate Substances 0.000 abstract description 2
- 229960001031 glucose Drugs 0.000 description 10
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 9
- 239000002304 perfume Substances 0.000 description 6
- 239000002994 raw material Substances 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 240000005373 Panax quinquefolius Species 0.000 description 3
- 235000013599 spices Nutrition 0.000 description 3
- 108020004465 16S ribosomal RNA Proteins 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 235000005979 Citrus limon Nutrition 0.000 description 2
- 244000131522 Citrus pyriformis Species 0.000 description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- SIKJAQJRHWYJAI-UHFFFAOYSA-N Indole Chemical compound C1=CC=C2NC=CC2=C1 SIKJAQJRHWYJAI-UHFFFAOYSA-N 0.000 description 2
- 102100021079 Ornithine decarboxylase Human genes 0.000 description 2
- PYMYPHUHKUWMLA-LMVFSUKVSA-N aldehydo-D-ribose Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 2
- 229940024606 amino acid Drugs 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 2
- 238000005660 chlorination reaction Methods 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000003912 environmental pollution Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 235000013336 milk Nutrition 0.000 description 2
- 239000008267 milk Substances 0.000 description 2
- 210000004080 milk Anatomy 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- MGADZUXDNSDTHW-UHFFFAOYSA-N 2H-pyran Chemical compound C1OC=CC=C1 MGADZUXDNSDTHW-UHFFFAOYSA-N 0.000 description 1
- 108010082340 Arginine deiminase Proteins 0.000 description 1
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- QNAYBMKLOCPYGJ-UHFFFAOYSA-N D-alpha-Ala Natural products CC([NH3+])C([O-])=O QNAYBMKLOCPYGJ-UHFFFAOYSA-N 0.000 description 1
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 1
- SRBFZHDQGSBBOR-SOOFDHNKSA-N D-ribopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@@H]1O SRBFZHDQGSBBOR-SOOFDHNKSA-N 0.000 description 1
- 241000192125 Firmicutes Species 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 229920002527 Glycogen Polymers 0.000 description 1
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 description 1
- QNAYBMKLOCPYGJ-UWTATZPHSA-N L-Alanine Natural products C[C@@H](N)C(O)=O QNAYBMKLOCPYGJ-UWTATZPHSA-N 0.000 description 1
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
- 102000004317 Lyases Human genes 0.000 description 1
- 108090000856 Lyases Proteins 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- OVRNDRQMDRJTHS-UHFFFAOYSA-N N-acelyl-D-glucosamine Natural products CC(=O)NC1C(O)OC(CO)C(O)C1O OVRNDRQMDRJTHS-UHFFFAOYSA-N 0.000 description 1
- OVRNDRQMDRJTHS-FMDGEEDCSA-N N-acetyl-beta-D-glucosamine Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O OVRNDRQMDRJTHS-FMDGEEDCSA-N 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 241000219100 Rhamnaceae Species 0.000 description 1
- NGFMICBWJRZIBI-JZRPKSSGSA-N Salicin Natural products O([C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@H](CO)O1)c1c(CO)cccc1 NGFMICBWJRZIBI-JZRPKSSGSA-N 0.000 description 1
- 240000008866 Ziziphus nummularia Species 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 230000009471 action Effects 0.000 description 1
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- 230000000996 additive effect Effects 0.000 description 1
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- 150000001298 alcohols Chemical class 0.000 description 1
- 150000001299 aldehydes Chemical class 0.000 description 1
- NGFMICBWJRZIBI-UHFFFAOYSA-N alpha-salicin Natural products OC1C(O)C(O)C(CO)OC1OC1=CC=CC=C1CO NGFMICBWJRZIBI-UHFFFAOYSA-N 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
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- 230000032050 esterification Effects 0.000 description 1
- 238000005886 esterification reaction Methods 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 235000019197 fats Nutrition 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000013355 food flavoring agent Nutrition 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 229940096919 glycogen Drugs 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- PZOUSPYUWWUPPK-UHFFFAOYSA-N indole Natural products CC1=CC=CC2=C1C=CN2 PZOUSPYUWWUPPK-UHFFFAOYSA-N 0.000 description 1
- RKJUIXBNRJVNHR-UHFFFAOYSA-N indolenine Natural products C1=CC=C2CC=NC2=C1 RKJUIXBNRJVNHR-UHFFFAOYSA-N 0.000 description 1
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 1
- 229960000367 inositol Drugs 0.000 description 1
- 239000010977 jade Substances 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical class CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 229960002160 maltose Drugs 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 229960001855 mannitol Drugs 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 150000002772 monosaccharides Chemical class 0.000 description 1
- 229950006780 n-acetylglucosamine Drugs 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 150000003222 pyridines Chemical class 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- NGFMICBWJRZIBI-UJPOAAIJSA-N salicin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC=CC=C1CO NGFMICBWJRZIBI-UJPOAAIJSA-N 0.000 description 1
- 229940120668 salicin Drugs 0.000 description 1
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
- 238000002864 sequence alignment Methods 0.000 description 1
- 230000000391 smoking effect Effects 0.000 description 1
- 235000010356 sorbitol Nutrition 0.000 description 1
- 229960002920 sorbitol Drugs 0.000 description 1
- 229960004793 sucrose Drugs 0.000 description 1
- 230000035900 sweating Effects 0.000 description 1
- 150000003505 terpenes Chemical class 0.000 description 1
- 235000007586 terpenes Nutrition 0.000 description 1
- 235000019505 tobacco product Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/24—Treatment of tobacco products or tobacco substitutes by extraction; Tobacco extracts
- A24B15/26—Use of organic solvents for extraction
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B3/00—Preparing tobacco in the factory
- A24B3/12—Steaming, curing, or flavouring tobacco
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
Landscapes
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of preparation method and application of cigarette with Semen Ziziphi Spinosae extractum, the method will plant raw Raoul bacterium seed culture fluid and food methanol bacillus cereuss seed culture fluid mixes, and obtain mixed bacteria liquid；Mixed bacteria liquid is inoculated into into fermentation in mixed extract and obtains fermentation liquid；To be fermented liquid precipitate sucking filtration, and filtrate reduced in volume is removed solvent, and prepared relative density is 1.20～1.36 cigarette Semen Ziziphi Spinosae extractum.The present invention is using the mixed extract of Semen Ziziphi Spinosae extracting solution, Grifola frondosa extracting solution and litchi nucleus extract fluid composition as sole carbon source, nitrogen source and substrate, containing alkaloid, triterpeness, flavonoid, 17 kinds of aminoacid etc., containing ferulic acid, vitamin C and plant sterol, cyclic adenosine monophosphate etc., it is easier to make microbial enzyme system fragrant using producing to ferment than single carbon source, wide material sources are easy to get, with the harmless advantage of natural environmental-protective.
Description
Technical field
The present invention relates to tobacco aromatics using technical field, specifically refers to a kind of preparation method of cigarette Semen Ziziphi Spinosae extractum and answers
With.
Background technology
In tobacco product, the fragrance and suction taste of Nicotiana tabacum L. is most important, and it is the key link in cigarette process to add spice,
And improve product style and improve the effective means of cigarette flavor quality.Tobacco aromatics using has chemically composite perfume and natural perfume material
Point.In recent years, natural plant pefume, because which is with Chinese characteristics, natural harmless, the reason such as raw material is easy to get, composition research and development weight
Point and focus.Microbial fermentation engineering is applied in the biosynthesiss of essence and flavoring agent, with Chinese herbal medicine as raw material, using micro-
Biology can produce various natural perfume materials, be a kind of good solution route for obtaining natural perfume material.
Semen Ziziphi Spinosae (Spina Date Seed), another name Semen Ziziphi Spinosae, Fructus Choerospondiatis, the mature seed of rhamnaceae plant wild jujube.Beginning is loaded in
《Sheng Nong's herbal classic》, top grade is classified as, nourishing the liver, mind calming are calmed the nerves, arresting sweating.Semen Ziziphi Spinosae contains alkaloid, triterpeness, flavonoid, 17 kinds of ammonia
Base acid etc., contains ferulic acid, vitamin C and plant sterol, cyclic adenosine monophosphate etc. again.But Semen Ziziphi Spinosae extract is used for Medicated cigarette, then
Can produce that zest is big, the significant deficiency more than miscellaneous QI, it is difficult to allocate cigarette use.
Microorganism can produce huge high activity enzyme system, such as polysaccharide hydrolysis enzyme, protease, esterification in breeding
Enzyme, redox enzymes and lyases etc., the collaboration of complicated metabolism in enzymatic catalysis, chemical action and microbial body are made
With under, the effect such as decomposed as raw material with Semen Ziziphi Spinosae, degraded, being aoxidized, being reduced, being polymerized, being coupled, being converted forms complicated low
Molecular compound, including various perfume compounds, such as alcohols, aldehydes, ketone, acids, lipid, phenols, pyran, pyridine
Class and terpenes etc., these aroma substances are also undoubtedly the aroma component of Nicotiana tabacum L. or the additive for Nicotiana tabacum L..
Chemosynthesis tobacco extract extraction step is complicated at present, and environmental pollution is serious, the fragrance single taste of tobacco extract
And aroma strength is still inadequate.Semen Ziziphi Spinosae fermented product extractum carries out cigarette and is reported with yet there are no disclosure.
The content of the invention
Complicated for current chemosynthesis tobacco extract extraction step, environmental pollution is serious, the fragrance taste of tobacco extract
The still inadequate defect of single and aroma strength, the invention provides a kind of preparation method and application of cigarette with Semen Ziziphi Spinosae extractum.
To achieve these goals, there is provided a kind of cigarette preparation method of Semen Ziziphi Spinosae extractum, comprise the following steps：
1) will filter after Semen Ziziphi Spinosae and water 1: 20～40 reflux, extract, in mass ratio 1～3 hour, filtrate is sterilized；Obtain final product
To Semen Ziziphi Spinosae extracting solution；
2) weigh by ratio of weight and the number of copies 40～80 parts of Semen Ziziphi Spinosae extracting solution, 20～40 parts of Grifola frondosa extracting solution and 5～
20 parts of litchi nucleus extract fluid, mix homogeneously obtain mixed extract；
3) plant raw Raoul bacterium actication of culture：Raw Raoul bacterium Raoultella planticola VP4-4, preservation will be planted
Numbering CCTCC NO:M 2012005 is inoculated on the raw Raoul bacterium slant medium of plant, at 25～38 DEG C, quiescent culture 24～
Activated spawn is obtained after 72h, is placed in refrigerator；
4) prepare and plant raw Raoul bacterium seed culture fluid：By step 3) in gained activated spawn, be inoculated into plant Sheng Lawu
That bacterium seed culture medium, under the conditions of 25～38 DEG C, 100～150r/min shaken cultivation 24h～72h is continuously transferred 1～3 time,
It is obtained and plants raw Raoul bacterium seed culture fluid；
5) eat methanol bacillus cereuss slant culture：Will food methanol bacillus
Methylotrophicus VJ4-1, deposit number CCTCC NO:M 2012004 is inoculated into potato glucose fine jade
On fat slant medium, under the conditions of 25～35 DEG C, pH5～9,24～72h of quiescent culture；
6) prepare food methanol bacillus cereuss seed culture fluid：With step 5) obtained by slant culture, be inoculated into food methanol
Bacillus cereuss seed culture medium, under the conditions of 25～35 DEG C, 100～150rpm shaken cultivation 24h～72h, continuously switching 1～4
It is secondary, food methanol bacillus cereuss seed culture fluid is obtained；
7) raw Raoul bacterium seed culture fluid will be planted and food methanol bacillus cereuss seed culture fluid is mixed by weight 1: 1～3
Close, obtain mixed bacteria liquid；
8) ferment：Mixed bacteria liquid 0.5～2% is inoculated in above-mentioned mixed extract by volume, postvaccinal mixing
The tinning amount of extracting solution be 20～40%, pH4～10,25～40 DEG C of temperature, 100～200rpm of rotating speed, not lucifuge condition issue
Ferment 7～14 days, obtains fermentation liquid；
9) it is 80～90% ethanol by volume 1: 2～6 by fermentation liquid and volume parts, sucking filtration after precipitates overnight will filter
Liquid concentrating under reduced pressure removes solvent, and prepared relative density is 1.20～1.36 cigarette Semen Ziziphi Spinosae extractum.
Preferably, the mixed extract is carried by 50～70 parts of Nicotiana tabacum L. extracting solution, 25～35 parts of Grifola frondosa
Take the litchi nucleus extract fluid composition of liquid and 8～18 parts.
Preferably, the mixed extract is by 60 parts of Nicotiana tabacum L. extracting solution, 30 parts of Grifola frondosa extracting solution and 10
The litchi nucleus extract fluid composition of part.
Preferably, the step 3) in, the life Raoul bacterium slant medium of planting is：Beerwort：120mL,
Sucrose：20g/L, agar：15g/L.
Preferably, the step 4) in, the sub- liquid culture medium of raw Raoul strain of planting is consisted of：Maltose：
50g/L, peptone：10g/L, Sodium Chloride：5g/L.
Preferably, the step 5) in, potato glucose slant medium：Rhizoma Solani tuber osi immersion 200mL/L, Portugal
Grape sugar 20g/L, agar 13g/L；
Preferably, the step 6) in, food methanol bacillus cereuss seed liquid culture medium is consisted of：Maltose 50g/
L, peptone 10g/L, Sodium Chloride 5g/L.
Present invention also offers a kind of application of gained cigarette with Semen Ziziphi Spinosae extractum, it is characterised in that：The cigarette Semen Ziziphi Spinosae
During extractum adds Medicated cigarette, for coordinating flue gas, increasing fragrant and sweet butter, the fragrance of a flower, make the soft comfortable, improvement of flue gas inhale taste, volume is added
In cigarette, the mass ratio of cigarette Semen Ziziphi Spinosae extractum is 0.01～0.05%.
Above-mentioned food methanol bacillus cereuss, the entitled Bacillus methylotrophicus VJ4-1 of the bacterial strain, in 2012
January 11 was preserved in Wuhan University's China typical culture collection center, deposit number CCTCC NO:M 2012004, which is biological
Be characterized in that：It is gram positive bacteria, can grow in 10%NaCl have arginine dihydrolase, ODC Ornithine decarboxylase to live
Property, acid can be produced, make milk liquefy；Can with N-Acetyl-D-glucosamine, Mannitol, glucose, salicin, D- 6-(.alpha.-D-galactosido)-D-glucose .s, D-ribose,
Inositol, sucrose, maltose, D-glucitol, L-arabinose, DL-LACTIC ACID salt, L-Alanine glycogen, L-PROLINE histidine, lemon
16S of the lemon hydrochlorate for growth, its 16S rRNA sequence and Bacillus methylotrophicus in the culture medium of carbon source
RRNA sequence alignment similaritys reach 100%.
Raoul bacterium, the entitled Raoultella planticola VP4-4 of the bacterial strain, in January 11 in 2012 are given birth in above-mentioned plant
It is preserved in Wuhan University's China typical culture collection center day, preserving number is CCTCC NO:M 2012005, plants raw Raoul
Bacterium Raoultella planticola VP4-4CCTCC NO:The biological property of M 2012005 is：Gram negative bacteria, energy
Produce acid, make milk liquefy and generate indole generation, E.C. 4.1.1.18 experiment to be positive；ODC Ornithine decarboxylase experiment, H2S are produced
Raw, gelatin liquefaction is negative；Can be grown for sole carbon source with monosaccharide, polysaccharide, esters and aminoacid,
The Molecular Identification for planting raw Raoul bacterium Raoultella planticola VP4-4 is characterized as：Its 16SrRNA sequence
Total length 1408bp, 16S rRNA sequences reach 99.563% with the similarity of Raoultella planticola.
It is an advantage of the invention that：
1st, the present invention using Semen Ziziphi Spinosae extracting solution, Grifola frondosa extracting solution and litchi nucleus extract fluid composition mixed extract as
Sole carbon source, nitrogen source and substrate, containing alkaloid, triterpeness, flavonoid, 17 kinds of aminoacid etc., containing ferulic acid, vitamin C and
Plant sterol, cyclic adenosine monophosphate etc., are easier to make microbial enzyme system fragrant using producing to ferment than single carbon source, and wide material sources are easy
, with the harmless advantage of natural environmental-protective.
2nd, using food methanol bacillus methylotrophicus VJ4-1, deposit number CCTCC NO：
It is sweet that M 2012004 and the raw Raoul bacterium Raoultella planticola of plant come fermenting and producing coordination flue gas, increase butter jointly
Perfume, the fragrance of a flower, make flue gas it is soft it is comfortable, improve the cigarette Semen Ziziphi Spinosae extractum for inhaling taste, can be with other essence spice for cigarette mixing preparations
Use.
3rd, the inventive method technical process is simple, and raw material sources extensively, have prospect useful industrially.
Specific embodiment
A part of embodiment is set forth below to be described in further detail the relevant technical problem of the present invention, it is necessary to
This points out that specific examples below is simply further illustrated to the present invention, does not represent limiting the scope of the invention.Its
Other people still fall within protection scope of the present invention according to some nonessential modifications and adjustment that the present invention makes.
Embodiment 1
The cigarette preparation method of Semen Ziziphi Spinosae extractum 1, comprises the following steps：
1) Semen Ziziphi Spinosae and water 1: 20 reflux, extract, in mass ratio are filtered after 3 hours, by filtrate in 115 DEG C of high temperature sterilizes 25
Minute, that is, obtain Semen Ziziphi Spinosae extracting solution；
2) Semen Litchi of 80 parts of Semen Ziziphi Spinosae extracting solution, 20 parts of Grifola frondosa extracting solution and 10 parts is weighed by ratio of weight and the number of copies
Extracting solution, mix homogeneously obtain mixed extract；
3) actication of culture：Raw Raoul bacterium Raoultella planticola VP4-4, deposit number CCTCC will be planted
NO:M 2012005 is inoculated on the raw Raoul bacterium slant medium of plant, at 38 DEG C, obtains activated spawn after quiescent culture 24h,
It is placed in 4 DEG C of refrigerators；Wherein, planting raw Raoul bacterium slant medium is：Beerwort：120mL, sucrose：20g/L, agar：15g/
L。
4) prepare and plant raw Raoul bacterium seed culture fluid：By step 3) in gained activated spawn, be inoculated into plant Sheng Lawu
That bacterium seed culture medium, under the conditions of 25 DEG C, 100r/min shaken cultivation 72h, continuous switching 3 times are obtained and plant life Raoul bacterium
Seed culture fluid；Wherein, plant the sub- liquid culture medium of raw Raoul strain to consist of：Maltose：50g/L, peptone：10g/L, chlorination
Sodium：5g/L.
5) eat methanol bacillus cereuss slant culture：Will food methanol bacillus methylotrophicus
VJ4-1, deposit number CCTCC NO:M 2012004 is inoculated in potato dextrose agar slant culture medium, in 30 DEG C, pH7
Under the conditions of, quiescent culture 48h；Wherein, potato glucose slant medium：Rhizoma Solani tuber osi immersion 200mL/L, glucose 20g/L,
Agar 13g/L；
6) prepare food methanol bacillus cereuss seed culture fluid：With step 5) obtained by slant culture, be inoculated into food methanol
Bacillus cereuss seed culture medium, under the conditions of 30 DEG C, 100rpm shaken cultivation 48h, continuous switching 2 times are obtained food methanol spore
Bacillus seed culture fluid；Wherein, eat methanol bacillus cereuss seed liquid culture medium to consist of：Maltose 50g/L, peptone 10g/L,
Sodium Chloride 5g/L；
7) raw Raoul bacterium seed culture fluid and food methanol bacillus cereuss seed culture fluid will be planted by weight 1: 1 mixing,
Obtain mixed bacteria liquid；
8) ferment：Mixed bacteria liquid 2% is inoculated into into above-mentioned mixed extract, the tinning amount of postvaccinal filtrate by volume
For 40%, in pH 4,40 DEG C of temperature, rotating speed 100rpm, not lucifuge condition bottom fermentation 14 days；Obtain fermentation liquid；
9) by step 8) in gained fermentation liquid and 95% ethanol by volume 1: 4, filtrate is subtracted by sucking filtration after precipitates overnight
Pressure concentration removes solvent, and prepared relative density is 1.20 cigarette Semen Ziziphi Spinosae extractum 1.
Embodiment 2
The cigarette preparation method of Semen Ziziphi Spinosae extractum 2, comprises the following steps：
1) by Semen Ziziphi Spinosae and water in mass ratio 1:Filter after 30 reflux, extract, 2 hours, by filtrate in 115 DEG C of high temperature sterilizes 25
Minute；Semen Ziziphi Spinosae extracting solution is obtained；
2) Semen Litchi of 60 parts of Semen Ziziphi Spinosae extracting solution, 30 parts of Grifola frondosa extracting solution and 10 parts is weighed by ratio of weight and the number of copies
Extracting solution, mix homogeneously obtain mixed extract；
3) actication of culture：Raw Raoul bacterium Raoultella planticola VP4-4, deposit number CCTCC will be planted
NO:M 2012005 is inoculated on the raw Raoul bacterium slant medium of plant, at 30 DEG C, obtains activated spawn after quiescent culture 72h,
It is placed in 4 DEG C of refrigerators；Wherein, planting raw Raoul bacterium slant medium is：Beerwort：120mL, sucrose：20g/L, agar：15g/
L；
4) prepare and plant raw Raoul bacterium seed culture fluid：By step 3) in gained activated spawn, be inoculated into plant Sheng Lawu
That bacterium seed culture medium, under the conditions of 32 DEG C, 100r/min shaken cultivation 48h, continuous switching 3 times are obtained and plant life Raoul bacterium
Seed culture fluid；Wherein plant the sub- liquid culture medium of raw Raoul strain to consist of：Maltose：50g/L, peptone：10g/L, chlorination
Sodium：5g/L；
5) eat methanol bacillus cereuss slant culture：Will food methanol bacillus methylotrophicus
VJ4-1, deposit number CCTCC NO:M 2012004 is inoculated in potato dextrose agar slant culture medium, in 25 DEG C, pH9
Under the conditions of, quiescent culture 24h；Wherein, potato glucose slant medium：Rhizoma Solani tuber osi immersion 200mL/L, glucose 20g/L,
Agar 13g/L；
6) prepare food methanol bacillus cereuss seed culture fluid：With step 5) obtained by slant culture, be inoculated into food methanol
Bacillus cereuss seed culture medium, under the conditions of 25 DEG C, 100rpm shaken cultivation 72h, continuous switching 4 times are obtained food methanol spore
Bacillus seed culture fluid；Wherein, eat methanol bacillus cereuss seed liquid culture medium to consist of：Maltose 50g/L, peptone 10g/L,
Sodium Chloride 5g/L；
7) raw Raoul bacterium seed culture fluid and food methanol bacillus cereuss seed culture fluid will be planted by weight 1: 3 mixing,
Obtain mixed bacteria liquid；
8) ferment：Mixed bacteria liquid 1% is inoculated into into above-mentioned mixed extract, the tinning amount of postvaccinal filtrate by volume
For 30%, in pH 8,30 DEG C of temperature, rotating speed 150rpm, lucifuge condition bottom fermentation 10 days, do not obtain fermentation liquid；
9) by step 8) in gained fermentation liquid and 95% ethanol by volume 1: 3, filtrate is subtracted by sucking filtration after precipitates overnight
Pressure concentration removes solvent, and prepared relative density is 1.36 cigarette Semen Ziziphi Spinosae extractum 2.
Embodiment 3
The cigarette preparation method of Semen Ziziphi Spinosae extractum 3, comprises the following steps：
1) by Semen Ziziphi Spinosae and water in mass ratio 1:Filter after 40 reflux, extract, 1 hour, by filtrate in 115 DEG C of high temperature sterilizes 25
Minute；Semen Ziziphi Spinosae extracting solution is obtained；
2) Semen Litchi of 70 parts of Semen Ziziphi Spinosae extracting solution, 35 parts of Grifola frondosa extracting solution and 15 parts is weighed by ratio of weight and the number of copies
Extracting solution, mix homogeneously obtain mixed extract；
3) actication of culture：Raw Raoul bacterium Raoultella planticola VP4-4, deposit number CCTCC will be planted
NO:M 2012005 is inoculated on slant medium, at 25 DEG C, obtains activated spawn, be placed in refrigerator after quiescent culture 24h；Its
In, slant medium is：Beerwort：120mL, sucrose：20g/L, agar：15g/L.
4) prepare and plant raw Raoul bacterium seed culture fluid：By step 3) in gained activated spawn, be inoculated into seed culture
Base, under the conditions of 25 DEG C, 100r/min shaken cultivation 72h, continuous switching 1 time are obtained and plant raw Raoul bacterium seed culture fluid；Its
In, plant the sub- liquid culture medium of raw Raoul strain and consist of：Maltose：50g/L, peptone：10g/L, Sodium Chloride：5g/L.
5) eat methanol bacillus cereuss slant culture：Will food methanol bacillus methylotrophicus
VJ4-1, deposit number CCTCC NO:M 2012004 is inoculated in potato dextrose agar slant culture medium, in 35 DEG C, pH5
Under the conditions of, quiescent culture 24h；Wherein, potato glucose slant medium：Rhizoma Solani tuber osi immersion 200mL/L, glucose 20g/L,
Agar 13g/L；
6) prepare food methanol bacillus cereuss seed culture fluid：With step 5) obtained by slant culture, be inoculated into food methanol
Bacillus cereuss seed culture medium, under the conditions of 35 DEG C, 150rpm shaken cultivation 24h, continuous switching 2 times are obtained food methanol spore
Bacillus seed culture fluid；Wherein, eat methanol bacillus cereuss seed liquid culture medium to consist of：Maltose 50g/L, peptone 10g/L,
Sodium Chloride 5g/L；
7) raw Raoul bacterium seed culture fluid and food methanol bacillus cereuss seed culture fluid will be planted by weight 1: 2 mixing,
Obtain mixed bacteria liquid；
8) ferment：Seed culture fluid 0.5% is inoculated into into above-mentioned mixed extract, the dress of postvaccinal filtrate by volume
Tank amount is 40%, in pH10,25 DEG C of temperature, and rotating speed 200rpm, not lucifuge condition bottom fermentation 7 days；Obtain fermentation liquid；
5) by step 8) in gained fermentation liquid and 95% ethanol by volume 1: 3, filtrate is subtracted by sucking filtration after precipitates overnight
Pressure concentration removes solvent, and prepared relative density is 1.30 cigarette Semen Ziziphi Spinosae extractum 3.
Embodiment 4
Above-mentioned cigarette is extracted in the ratio of tobacco shred amount 0.5%, 0.1%, 0.05%, Semen Ziziphi Spinosae by inventor with Semen Ziziphi Spinosae extractum
Thing extractum is smoked panel test in being respectively added to tobacco shred in tobacco shred amount ratio 0.01%, smokes panel test according to GB GB 5606.4-1996
Carry out.Smoking result is as shown in table 2 below.Jing expert group of smokeing panel test smokes panel test repeatedly, it is believed that：This cigarette Semen Ziziphi Spinosae extractum, with association
Adjust flue gas, increase fragrant and sweet butter, the fragrance of a flower, soft advantage that is comfortable, improving suction taste, can be with other essence spice for cigarette mixing preparations
Use, improve Semen Ziziphi Spinosae extract extractum and be added in Medicated cigarette, miscellaneous QI is more, the shortcoming that zest is big.
Application of 2. cigarette of table with Semen Ziziphi Spinosae extractum in cigarette charging
Claims (8)
1. preparation method of a kind of cigarette with Semen Ziziphi Spinosae extractum, it is characterised in that：Comprise the following steps：
1) will filter after Semen Ziziphi Spinosae and water 1: 20～40 reflux, extract, in mass ratio 1～3 hour, filtrate is sterilized；Acid is obtained
Semen Ziziphi Spinosae extracting solution；
2) 40～80 parts of Semen Ziziphi Spinosae extracting solution, 20～40 parts of Grifola frondosa extracting solution and 5～20 parts are weighed by ratio of weight and the number of copies
Litchi nucleus extract fluid, mix homogeneously obtains mixed extract；
3) plant raw Raoul bacterium actication of culture：Raw Raoul bacterium Raoultella planticola VP4-4CCTCC M will be planted
2012005 are inoculated on the raw Raoul bacterium slant medium of plant, at 25～38 DEG C, obtain activating bacterium after 24～72h of quiescent culture
Kind, it is placed in refrigerator；
4) prepare and plant raw Raoul bacterium seed culture fluid：By step 3) in gained activated spawn, be inoculated into the raw Raoul bacterium of plant
Seed culture medium, under the conditions of 25～38 DEG C, 100～150r/min shaken cultivation 24h～72h is continuously transferred 1～3 time, is obtained
Plant raw Raoul bacterium seed culture fluid；
5) eat methanol bacillus cereuss slant culture：Will food methanol bacillus methylotrophicus CCTCC
M 2012004 is inoculated in potato dextrose agar slant culture medium, under the conditions of 25～35 DEG C, pH5～9, quiescent culture
24～72h；
6) prepare food methanol bacillus cereuss seed culture fluid：With step 5) obtained by slant culture, be inoculated into food methanol spore
Bacillus seed culture medium, under the conditions of 25～35 DEG C, 100～150rpm shaken cultivation 24h～72h is continuously transferred 1～4 time, system
Methanol bacillus cereuss seed culture fluid must be eaten；
7) raw Raoul bacterium seed culture fluid and food methanol bacillus cereuss seed culture fluid will be planted by weight 1: 1～3 mixing, is obtained
To mixed bacteria liquid；
8) ferment：Mixed bacteria liquid 0.5～2% is inoculated in above-mentioned mixed extract by volume, postvaccinal mixed extraction
The tinning amount of liquid is 20～40%, pH4～10,25～40 DEG C of temperature, 100～200rpm of rotating speed, not lucifuge condition bottom fermentation 7～
14 days, obtain fermentation liquid；
9) it is 80～90% ethanol by volume 1: 2～6 by fermentation liquid and volume parts, filtrate is subtracted by sucking filtration after precipitates overnight
Pressure concentration removes solvent, and prepared relative density is 1.20～1.36 cigarette Semen Ziziphi Spinosae extractum.
2. preparation method of the cigarette with Semen Ziziphi Spinosae extractum according to claim 1, it is characterised in that：The step 2) in, mixing
Litchi nucleus extract fluid group of the extracting solution by 50～70 parts of Nicotiana tabacum L. extracting solution, 25～35 parts of Grifola frondosa extracting solution and 8～18 parts
Into.
3. preparation method of the cigarette with Semen Ziziphi Spinosae extractum according to claim 2, it is characterised in that：The step 2) in, mixing
Extracting solution is made up of the litchi nucleus extract fluid of 60 parts of Nicotiana tabacum L. extracting solution, 30 parts of Grifola frondosa extracting solution and 10 parts.
4. preparation method of the cigarette with Semen Ziziphi Spinosae extractum according to Claims 2 or 3, it is characterised in that：The step 3) in, institute
Stating the raw Raoul bacterium slant medium of plant is：Beerwort：120mL, sucrose：20g/L, agar：15g/L.
5. preparation method of the cigarette with Semen Ziziphi Spinosae extractum according to Claims 2 or 3, it is characterised in that：The step 4) in, institute
State the sub- liquid culture medium of the raw Raoul strain of plant to consist of：Maltose：50g/L, peptone：10g/L, Sodium Chloride：5g/L.
6. preparation method of the cigarette with Semen Ziziphi Spinosae extractum according to Claims 2 or 3, it is characterised in that：The step 5) in, horse
Bell potato glucose slant medium：Rhizoma Solani tuber osi immersion 200mL/L, glucose 20g/L, agar 13g/L.
7. preparation method of the cigarette with Semen Ziziphi Spinosae extractum according to Claims 2 or 3, it is characterised in that：The step 6) in, food
Methanol bacillus cereuss seed liquid culture medium is consisted of：Maltose 50g/L, peptone 10g/L, Sodium Chloride 5g/L.
8. a kind of according to the application of Semen Ziziphi Spinosae extractum of claim 1 gained cigarette, it is characterised in that：Cigarette Semen Ziziphi Spinosae extractum
In adding Medicated cigarette, for coordinating flue gas, increasing fragrant and sweet butter, the fragrance of a flower, make the soft comfortable, improvement of flue gas inhale taste, in adding Medicated cigarette
The mass ratio of cigarette Semen Ziziphi Spinosae extractum is 0.01～0.05%.
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