CN106720952A - It is a kind of to utilize micro- corn core carrier low cost production specific yolk antibody preparation and preparation method cut and help interaction mode to process - Google Patents
It is a kind of to utilize micro- corn core carrier low cost production specific yolk antibody preparation and preparation method cut and help interaction mode to process Download PDFInfo
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- CN106720952A CN106720952A CN201611185405.2A CN201611185405A CN106720952A CN 106720952 A CN106720952 A CN 106720952A CN 201611185405 A CN201611185405 A CN 201611185405A CN 106720952 A CN106720952 A CN 106720952A
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- yolk antibody
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
A kind of to utilize micro- corn core carrier low cost production specific yolk antibody preparation and preparation method cut and help interaction mode to process, by mass percentage, the product is made up of following components：Specific band shell shell egg 30% 80%, with micro- corn core carrier 20% 70% cut and help interaction technical finesse, potassium sorbate 0.05% 0.2%, calcium propionate 0.1% 0.5%.Product of the invention is suitable to the animals such as direct feeding stichopus japonicus, turbot, milk cow and piglet, can be used to prevent and treat bacterium infection or other immunological diseases, greatly improves the survival rate and immunity of animal.The production technology of product of the present invention is greatly simplified, and production cost is greatly lowered, and is more suitable for large-scale production.In addition, the material such as calcium, magnesium in eggshell can additionally supplement animal needed for trace element, for strengthening the immunity of animal, promoting the growth of animal there is certain effect.
Description
Technical field
The invention belongs to biological feedstuff technical field, it is related to a kind of new for the special of Animal diseases prevention and treatment
Property yolk antibody preparation formula and preparation method.
Background technology
Due to medicament residue caused by the use of antibiotic, it has also become the aquatic products product competitiveness in the international market of restriction China
A subject matter.IgY (Yolk antibody) has the advantages that nontoxic, noresidue, does not develop immunity to drugs, and development prospect is enjoyed looks steadily
Mesh.In addition IgY preparation costs are low, output capacity is high, purification is simple and efficient, and have the advantages that heat-resisting, good stability, wide
Product is made generally to carry out the prevention and treatment of Animal diseases.For example sea cucumber is prevented and treated with the IgY preparations being made come feeding sea cucumber
Skin ulcer syndrome etc..
Current IgY products are mostly freeze-dried powder or sheet, ointment preparation, but freeze-dried powder and sheet, ointment preparation
Preparation technology it is complicated, it is relatively costly, and active ingredient has greater loss during purification is prepared.Examined based on more than
Consider, we directly by the egg containing special yolk antibody and cut the corn core carrier knot for helping interaction technical finesse by micro-
Close, be made feed addictive, not only solve the problems, such as loss of effective components but also preparation technology is greatly simplified, save a large amount of energy
Source, production cost is greatly lowered.
By micro- preparation cut and help interaction technology for feed addictive, fracturing cell walls and modified natural active material are
Develop a new application field of starting in recent years.Micro- cutting helps interaction technology to come from mechanochemistry, and its general principle is in powder
During broken, by micro- shear, cell membrane is crushed solid material cluster of grains, and cell surface produces fresh tangent plane, chemical assistant with
There is group or intermolecular mutual absorption or effect between active ingredient, change the micro-property of effective ingredients in plant, surface
Can increase, specific surface area increase, adsorptivity, polarity increase, change its dissolubility, and then improve the biology profit of target effective composition
Expenditure.
The content of the invention
Inventor has thought better of the purification preparation method of characteristic Yolk antibody from cost-effective, and therefrom inhales
The experience of preciousness is taken, is directly mixed with by micro- corn core carrier for helping interaction treated of cutting using by specific band shell egg pulp
Method, product is not only functionally fully met requirement, and to save and need consumption in Yolk antibody purification process
Big energy, reduces production cost.
The present invention is adopted the following technical scheme that：A kind of specific yolk antibody preparation, according to mass ratio, by following component groups
Into：
Described micro- corn core carrier of cutting is helped during interaction technical finesse maize cob meal with micro- cutting, and the auxiliary agent for using is carbon
Sour calcium or calcium bicarbonate, auxiliary dosage are 1%-8% (w/w), and the ultramicro grinding time is 5min-15min.
The quality proportioning of specific band shell shell egg and maize cob meal is 1:1-3:1.
The preparation method of described specific yolk antibody preparation, comprises the following steps：
(1) by maize cob meal with it is micro- cut help interaction technical finesse after, cross 60-200 mesh sieve it is standby；
(2) after high-immunity egg is cleaned, band shell is squeezed into container, is stirred；
(3) specific band shell egg pulp is stirred to volumetric expansion, lighter, in thick；
(4) addition potassium sorbate, calcium propionate, stir；
(5) by the corn core carrier addition egg yolk liquid after sieving, it is stirred until homogeneous, forms neither too hard, nor too soft bulk and consolidate
Body, is made soft particle
(6) dry at room temperature；
(7) pulverize and sieve；
(8) it is made feed addictive.
Compared with prior art, the beneficial effects of the invention are as follows：
The present invention plays the purpose of optimum efficiency and energy saving reduces cost from IgY, changes traditional system
The method of standby IgY products, is added in the middle of feed after IgY no longer is made into freeze-dried powder or purification as feed addictive.
But directly hybrid process will be carried out containing the specific band shell egg pulp rich in specific IgY and maize cob meal, so not only
Reduce separating, in purification and freeze-drying process IgY loss, and also simplify production technology, this product is critically important
It is exactly a little that natural corn core carrier needs largely to consume the difficulty of the energy in solving conventional Yolk antibody production purification process
Topic, greatly reduces cost.
The various methods of table 1 produce IgY preparation Integrated comparatives
As it can be seen from table 1 in the case where the factors such as cost of labor and equipment depreciation are not considered, this method early investment
Equipment cost it is minimum, it is even more many that specific energy consumption falls below original 1/5, and significantly, and production process is simple for energy-saving effect,
Flexibly, restraining factors are less, with very strong actual application value for the mode of production.
Brief description of the drawings
Fig. 1 is the flow chart of low cost production specific yolk antibody preparation；
Fig. 2 is conventional corn slug particle figure；
Fig. 3 is micro- corn core carrier cut and help interaction technical finesse；
Fig. 4 standard IgY curves；
The light absorption value of Fig. 5 different times IgY preparations.
Specific embodiment
Embodiments of the invention are described in detail below in conjunction with technical scheme.
Embodiment 1
Corncob meal is carried out into ultramicro grinding, auxiliary agent is added during ultramicro grinding, the auxiliary agent for using is calcium carbonate, auxiliary agent is used
Measure is 5% (w/w), grinding time 10min.
Contrasted by stereoscan photograph, it can be found that using micro- corn core carrier cut after helping interaction technical finesse
Its surface is more coarse, and surface area becomes much larger, and it is stronger that corresponding its combines adsorption capacity.Experiment shows, relative to common jade
Rice core carrier, 30%-100% can be increased using micro- corn core carrier adsorption capacity cut after helping interaction technical finesse.
Embodiment 2
By maize cob meal with it is micro- cut help interaction technical finesse after, cross 100 mesh sieve, weigh 300g standby；Will be by injection
High-immunity egg after inactivation Huanghai Sea Shewanella is immune, takes yolk 600g and squeezes into container with Yolk separator, stirs；By egg yolk liquid
It is stirred to volumetric expansion, lighter, in thick；Addition 0.9g potassium sorbates, 2.7g calcium propionates, stir；Will sieving
Maize cob meal afterwards is added in band shell egg pulp, is stirred until homogeneous, and forms the moderate lumps solid of soft durometer；Then with system
Grain machine is made the soft particles of diameter 3mm；Dry at room temperature；100 mesh sieve, packaging are crossed after being crushed with pulverizer；It is made feed addition
Agent.
Embodiment 3
By maize cob meal with it is micro- cut help interaction technical finesse after, cross 60 mesh sieve, weigh 500g standby；To be gone out by injection
High-immunity egg after Vibrio splindidus living are immune, takes yolk 900g and squeezes into container with Yolk separator, stirs；Egg yolk liquid is stirred to
Volumetric expansion, lighter, in thick；Addition 1.5g potassium sorbates, 4.5g calcium propionates, stir；By the jade after sieving
Rice core powder is added in band shell egg pulp, is stirred until homogeneous, and forms the moderate lumps solid of soft durometer；Then granulation mechanism is used
Into the soft particles of diameter 3mm；Dry at room temperature；100 mesh sieve, packaging are crossed after being crushed with pulverizer；It is made feed addictive.
Embodiment 4
IgY content detections
Obtained IgY preparations 1g, plus 30ml coating buffers are weighed, ultrasonic oscillation 15min after 100 times of dilution, adds per hole
100ul is coated with 96 hole elisa Plates, and 4 DEG C overnight.ELISA Plate is washed with cleaning solution 3 times, 100ul confining liquids, 37 DEG C of incubations are added per hole
Dried after 2h, washed 3 times, then add rabbit-anti chicken IgG-HRP100ul, 37 DEG C of reaction lh to each hole；Cleaning solution washs each hole 5 times, often
Hole adds 100ul tmb substrate buffer solutions, and develop the color 20min at room temperature, and 50ul 2M sulfuric acid terminating reactions are added per hole, is surveyed with ELIASA
OD450/630nm.Then with standard IgY curve comparisons, the specific IgY content in different times sample is calculated.
As seen from Figure 4, this product has no in half a year internal specific IgY content and substantially reduces and maintain shape higher
State, illustrate this product can in longer time continuous and effective.
Claims (4)
1. a kind of using micro- corn core carrier low cost production specific yolk antibody preparation cut and help interaction mode to process, it is special
Levy and be, each component is configured by following mass percent：
2. a kind of utilization according to claim 1 is micro- cuts the corn core carrier low cost production for helping interaction mode to process specifically
Property yolk antibody preparation, it is characterised in that described micro- corn core carrier of cutting helps interaction technical finesse maize cob meal mistake with micro- cutting
Cheng Zhong, the auxiliary agent for using is calcium carbonate or calcium bicarbonate, and auxiliary dosage is 1%-8% (w/w), and the ultramicro grinding time is 5min-
15min。
3. a kind of utilization according to claim 1 is micro- cuts the corn core carrier low cost production for helping interaction mode to process specifically
Property yolk antibody preparation, it is characterised in that the quality proportioning of specific band shell shell egg and maize cob meal be 1:1-3:1.
4. a kind of preparation method of specific yolk antibody preparation as claimed in claim 1, comprises the following steps：
(1) by maize cob meal with it is micro- cut help interaction technical finesse after, cross 60-200 mesh sieve it is standby；
(2) after the height of collection being exempted from into egg cleaning, band shell is squeezed into container, is stirred；
(3) specific band shell egg pulp is stirred to volumetric expansion, lighter, in thick；
(4) addition potassium sorbate, calcium propionate, stir；
(5) by sieving after it is micro- cut corn core carrier add specific band shell egg pulp in, stir, formed it is neither too hard, nor too soft
Bulk solid；
(6) soft particle is made of granulator；
(7) dry at room temperature；
(8) separating twice sieving；
(9) finished product.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611185405.2A CN106720952A (en) | 2016-12-20 | 2016-12-20 | It is a kind of to utilize micro- corn core carrier low cost production specific yolk antibody preparation and preparation method cut and help interaction mode to process |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611185405.2A CN106720952A (en) | 2016-12-20 | 2016-12-20 | It is a kind of to utilize micro- corn core carrier low cost production specific yolk antibody preparation and preparation method cut and help interaction mode to process |
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| CN106720952A true CN106720952A (en) | 2017-05-31 |
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| CN201611185405.2A Pending CN106720952A (en) | 2016-12-20 | 2016-12-20 | It is a kind of to utilize micro- corn core carrier low cost production specific yolk antibody preparation and preparation method cut and help interaction mode to process |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113307825A (en) * | 2021-05-24 | 2021-08-27 | 浙江工业大学 | Method for extracting calcium phytate from rice bran waste residues |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102696922A (en) * | 2012-06-14 | 2012-10-03 | 昆山贝瑞康生物科技有限公司 | Intestine protecting compound for sea cucumbers and preparation thereof |
| CN105198989A (en) * | 2015-09-30 | 2015-12-30 | 大连理工大学 | Shewanella-smarisflavi-resistant egg yolk antibody and preparation method thereof |
-
2016
- 2016-12-20 CN CN201611185405.2A patent/CN106720952A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102696922A (en) * | 2012-06-14 | 2012-10-03 | 昆山贝瑞康生物科技有限公司 | Intestine protecting compound for sea cucumbers and preparation thereof |
| CN105198989A (en) * | 2015-09-30 | 2015-12-30 | 大连理工大学 | Shewanella-smarisflavi-resistant egg yolk antibody and preparation method thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113307825A (en) * | 2021-05-24 | 2021-08-27 | 浙江工业大学 | Method for extracting calcium phytate from rice bran waste residues |
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Application publication date: 20170531 |
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