CN106578357A - Specificity egg yolk antibody preparation produced by combining ultramicro-pulverized zeolite powder and specificity egg yolk liquid and preparation method thereof - Google Patents
Specificity egg yolk antibody preparation produced by combining ultramicro-pulverized zeolite powder and specificity egg yolk liquid and preparation method thereof Download PDFInfo
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- CN106578357A CN106578357A CN201611185362.8A CN201611185362A CN106578357A CN 106578357 A CN106578357 A CN 106578357A CN 201611185362 A CN201611185362 A CN 201611185362A CN 106578357 A CN106578357 A CN 106578357A
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- zeolite powder
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Abstract
The invention provides a specificity egg yolk antibody preparation produced by combining ultramicro-pulverized zeolite powder and specificity egg yolk liquid and a preparation method thereof. The product provided by the invention is prepared from the following components in percentage by mass: 20 percent to 80 percent of the specificity egg yolk liquid, 20 percent to 80 percent of an ultramicro-pulverized zeolite powder carrier, 0.05 percent to 0.2 percent of potassium sorbate and 0.1 percent to 0.5 percent of calcium propionate. The product provided by the invention is suitable for directly feeding animals including stichopus japonicus, turbots, dairy cattle, piglets and the like, and can be used for preventing and treating bacterial infection or other immunological diseases, so that the survival rate and immunity of the animals are greatly improved. Furthermore, a production process of the product is greatly simplified and the production cost is greatly reduced, so that the production process is more suitable for large-scale production.
Description
Technical field
The invention belongs to biological feedstuff technical field, is related to a kind of special yolk for Animal diseases prevention and treatment
Antibody preparation formula and preparation method.
Background technology
The caused drug residue due to the use of antibiotic, it has also become restriction China Aquatic product product competitiveness in the international market
A subject matter.IgY (yolk antibody) has the advantages that nontoxic, noresidue, does not develop immunity to drugs, and development prospect is enjoyed looks steadily
Mesh.In addition IgY preparation costs are low, output capacity is high, purification is simple and efficient, and have the advantages that heat-resisting, good stability, wide
Make product generally to carry out the prevention and treatment of Animal diseases.IgY preparations prevent and treat Stichopus japonicuss come feeding Stichopus japonicuss for example with made by
Skin ulcerative syndrome etc..
Current IgY products are mostly lyophilized powder or lamellar, ointment preparation, but lyophilized powder and lamellar, ointment preparation
Complicated process of preparation, it is relatively costly, and effective ingredient prepare purification during have greater loss.Examine based on more than
Consider, we are directly combined the special yolk liquid containing special yolk antibody with the zeolite powder of micronizing, are made feedstuff and are added
Plus agent, not only solve the problems, such as loss of effective components but also preparation technology is greatly simplified, save mass energy, production cost is big
To reduce.
The content of the invention
Inventor has thought better of the purification preparation method of characteristic yolk antibody from cost-effective, and therefrom inhales
The experience of preciousness is taken, the method directly mixed with special yolk liquid using the zeolite powder of micronizing makes product not only in work(
Can on fully meet requirement, and save need in yolk antibody purification process consume big energy, reduce and produce into
This.
A kind of specific yolk antibody preparation of the present invention, according to mass ratio, is made up of following components:
In micronizing, the auxiliary agent for using is sodium carbonate or sodium bicarbonate, and auxiliary dosage is 1%-8% (w/w), ultra micro
Grinding time is 3min-15min.
The quality proportioning of special yolk liquid and micronizing zeolite powder is 1:4-3:1.
A kind of preparation method of described specific yolk antibody preparation, comprises the steps:
(1) by after zeolite powder powder micronizing, 60-200 mesh sieve is crossed standby;
(2) high-immunity egg Yolk separator is isolated into egg yolk to squeeze in container, stirs;
(3) special yolk liquid is stirred to into volumetric expansion, lighter, in thick;
(4) add potassium sorbate, calcium propionate, stir;
(5) zeolite powder carrier after sieving is added in special yolk liquid, is stirred, and forms neither too hard, nor too soft bulk
Solid;
(6) soft granule is made with granulator;
(7) dry under room temperature;
(8) separating twice sieves;
(9) feed additive is made.
Compared with prior art, the invention has the beneficial effects as follows:
The present invention plays the purpose of optimum efficiency and energy saving reduces cost from IgY, changes traditional system
The method of standby IgY products, is added in the middle of feedstuff after no longer IgY being made into lyophilized powder or purification as feed additive.
But directly hybrid process will be carried out with the zeolite powder of micronizing containing the special yolk liquid rich in specific IgY, so
The loss of the IgY in separation, purification and freeze-drying process is not only reduced, and also simplify production technology, this product is very heavy
What is wanted is exactly a little that natural zeolite powder carrier is solved to be needed to consume the energy in a large number in conventional yolk antibody production purification process
A difficult problem, greatly reduce cost.
The various methods of table 1 produce IgY preparation Integrated comparatives
As it can be seen from table 1 in the case where the factors such as cost of labor and equipment depreciation are not considered, this method early investment
Equipment cost it is minimum, it is even more many that specific energy consumption falls below original 1/5, and significantly, and production process is simple for energy-saving effect,
Flexibly, restraining factors are less, with very strong actual application value for the mode of production.
Description of the drawings
Fig. 1 is the flow chart of low cost production specific yolk antibody preparation;
Fig. 2 is common zeolite powder scanning electron microscope (SEM) photograph;
Fig. 3 is micronizing zeolite powder scanning electron microscope (SEM) photograph;
Fig. 4 is standard IgY curve;
Fig. 5 is the light absorption value of different times IgY preparations.
Specific embodiment
The specific embodiment of the present invention is described in detail below in conjunction with technical scheme.
Embodiment 1
Zeolite powder coarse powder is carried out into micronizing, auxiliary agent is added during micronizing, the auxiliary agent for using is sodium carbonate, auxiliary agent is used
Measure as 5% (w/w), grinding time 10min.
Contrasted by stereoscan photograph, it can be found that zeolite powder is after micronizing, its surface pore is more,
Surface area becomes much larger, and it is higher that corresponding its combines absorbability.Experiment shows, relative to common zeolite powder, using superfine powder
Zeolite powder carrier absorbability after broken process can increase 30%-90%.
Embodiment 2
By zeolite powder after micronizing process, 100 mesh sieve are crossed, weigh 300g standby;Will be yellow through injection inactivation
High-immunity egg after extra large Shewanella immunity, takes egg yolk 300g and squeezes in container with Yolk separator, stirs;Egg yolk liquid is stirred to
Volumetric expansion, lighter, in thick;Addition 0.6g potassium sorbate, 1.8g calcium propionates, stir;By the boiling after sieving
Stone powder is added in special yolk liquid, is stirred until homogeneous, and forms the moderate lumps solid of soft durometer;Then granulation mechanism is used
Into the soft granules of diameter 3mm;Dry under room temperature;100 mesh sieve, packaging are crossed after being crushed with pulverizer;Make feed additive.
Embodiment 3
By zeolite powder after micronizing process, 60 mesh sieve are crossed, weigh 500g standby;Will be magnificent through injection inactivation
High-immunity egg after vibrio immunity, takes yolk 900g and squeezes in container with Yolk separator, stirs;Yolk liquid is stirred to into volume swollen
It is swollen, lighter, in thick;Addition 1.5g potassium sorbate, 4.5g calcium propionates, stir;Zeolite powder after sieving is added
Enter in yolk liquid, be stirred until homogeneous, form the moderate lumps solid of soft durometer;Then diameter 3mm is made with granulator soft
Granule;Dry under room temperature;100 mesh sieve, packaging are crossed after being crushed with pulverizer;Make feed additive.
Embodiment 4
IgY content detection
Obtained IgY preparations 1g, plus 30ml coating buffers are weighed, ultrasonic wave concussion 15min after 100 times of dilution, adds per hole
100ul is coated with 96 hole elisa Plates, and 4 DEG C overnight.ELISA Plate 3 times is washed with cleaning mixture, 100ul confining liquids, 37 DEG C of incubations are added per hole
Dry after 2h, wash 3 times, then add rabbit-anti chicken IgG-HRP100ul, 37 DEG C of reaction lh to each hole;The each hole of cleaning mixture washing 5 times, often
Hole adds 100ul tmb substrate buffer, and develop the color 20min under room temperature, and per hole 50ul 2M sulphuric acid terminating reactions are added, and is surveyed with microplate reader
OD450/630nm.Then with standard IgY curve comparison, the specific IgY content in different times sample is calculated.
As seen from Figure 4, this product has no in half a year internal specific IgY content and substantially reduces and maintain higher shape
State, illustrate this product can in longer time continuous and effective.
Claims (4)
1. a kind of zeolite powder of utilization micronizing is combined the specific yolk antibody preparation of production with special yolk liquid, and it is special
Levy and be, each component is configured by following mass percent:
2. the zeolite powder of utilization micronizing according to claim 1 is combined the specificity ovum of production with special yolk liquid
Yellow antibody preparation, it is characterised in that in micronizing, the auxiliary agent for using is sodium carbonate or carbon to described micronizing zeolite powder
Sour hydrogen sodium, auxiliary dosage is 1%-8% (w/w), and the micronizing time is 3min-15min.
3. the zeolite powder of utilization micronizing according to claim 1 is combined the specificity ovum of production with special yolk liquid
Yellow antibody preparation, it is characterised in that the quality proportioning of special yolk liquid and micronizing zeolite powder is 1:4-3:1.
4. a kind of preparation method of specific yolk antibody preparation as claimed in claim 1, it is characterised in that including following step
Suddenly:
(1) by after zeolite powder powder micronizing, 60-200 mesh sieve is crossed standby;
(2) high-immunity egg Yolk separator is isolated into egg yolk to squeeze in container, stirs;
(3) special yolk liquid is stirred to into volumetric expansion, lighter, in thick;
(4) add potassium sorbate, calcium propionate, stir;
(5) zeolite powder carrier after sieving is added in special yolk liquid, is stirred, and is formed neither too hard, nor too soft bulk and is consolidated
Body;
(6) soft granule is made with granulator;
(7) dry under room temperature;
(8) separating twice sieves;
(9) feed additive is made.
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CN201611185362.8A CN106578357A (en) | 2016-12-20 | 2016-12-20 | Specificity egg yolk antibody preparation produced by combining ultramicro-pulverized zeolite powder and specificity egg yolk liquid and preparation method thereof |
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CN201611185362.8A CN106578357A (en) | 2016-12-20 | 2016-12-20 | Specificity egg yolk antibody preparation produced by combining ultramicro-pulverized zeolite powder and specificity egg yolk liquid and preparation method thereof |
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Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102429097A (en) * | 2011-07-25 | 2012-05-02 | 大连赛姆生物工程技术有限公司 | Food therapy yolk antibody yolk liquid pasty preparation for animals and production method thereof |
CN105146135A (en) * | 2015-07-22 | 2015-12-16 | 湖州海皇生物科技有限公司 | Compound feed capable of improving stress resistance of pelteobagrus fulvidraco |
CN105198988A (en) * | 2015-09-30 | 2015-12-30 | 大连理工大学 | Vibrio-splendidus-resistant egg yolk antibody and preparation method thereof |
-
2016
- 2016-12-20 CN CN201611185362.8A patent/CN106578357A/en active Pending
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102429097A (en) * | 2011-07-25 | 2012-05-02 | 大连赛姆生物工程技术有限公司 | Food therapy yolk antibody yolk liquid pasty preparation for animals and production method thereof |
CN105146135A (en) * | 2015-07-22 | 2015-12-16 | 湖州海皇生物科技有限公司 | Compound feed capable of improving stress resistance of pelteobagrus fulvidraco |
CN105198988A (en) * | 2015-09-30 | 2015-12-30 | 大连理工大学 | Vibrio-splendidus-resistant egg yolk antibody and preparation method thereof |
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Application publication date: 20170426 |
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