CN106688362A - Germination hastening method for camellia japonica seeds - Google Patents
Germination hastening method for camellia japonica seeds Download PDFInfo
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- CN106688362A CN106688362A CN201611041401.7A CN201611041401A CN106688362A CN 106688362 A CN106688362 A CN 106688362A CN 201611041401 A CN201611041401 A CN 201611041401A CN 106688362 A CN106688362 A CN 106688362A
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- vernalization
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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- Environmental Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention mainly relates to the technical field of planting and discloses a germination hastening method for camellia japonica seeds. The method comprises the steps of carrying out soaking, carrying out enzymolysis, carrying out fermentation and carrying out germination hastening. The method is simple, rapid and efficient, the germination hastening ratio of the camellia japonica seeds reaches 98%, the germination hastening time is shortened to 3 to 4 days, the survival rate reaches 96%, and the economic income is increased by 11.3%; through clean-water soaking, wizened seeds are removed, and seed coats are softened; through enzymolysis, seed coat structures of the camellia japonica seeds can be further destroyed, the entry of nutritional ingredients is promoted, and the seed germination is accelerated; through subjecting the seeds to fermentation by a brewer's yeast and acetic bacteria, the seed coats and dormant substances are destroyed, the stress resistance of embryonic buds is improved, and the survival rate is increased; and through placing the seeds in a germination hastening box, gradually regulating germination hastening conditions and sprinkling a chitosan solution to maintain humidity, ways of application of chitosan are increased, the stress resistance of the embryonic buds is improved, the growth of the embryonic buds is accelerated, and the germinating time is shortened.
Description
Technical field
The invention mainly relates to planting technology field, more particularly to a kind of germination accelerating method of camellia seeds.
Background technology
Camellia, various regions are cultivated extensively, and camellia designs and varieties are various, and it is red or pale red to spend most of, also there is white, many
It is polyphyll, flower has hemostasia effect, seed oil expression, for industrial.Tea oil golden yellow color is pale yellow, and quality is pure, clear, gas
Taste delicate fragrance, good taste, both oral administrations, also can external application, can build up resistance, enhancing development, cosmetic hair-care protects the heart
The cerebrovascular, anti-aging.
It is main in the planting process of camellia to use cutting propagation, seminal propagation using less, but when new varieties are cultivated,
Seminal propagation is often used, but seed after planting needs to germinate for 15 ~ 20 days at present, germinating time is long, and plantation efficiency is low,
Therefore need to carry out seed vernalization, but the only traditional infusion method of the vernalization of camellia seeds and sand bury method now, do not have also
The germination accelerating method of camellia seeds is recorded and reported to special document.
The content of the invention
In order to make up the defect of prior art, it is an object of the invention to provide a kind of germination accelerating method of camellia seeds.
A kind of germination accelerating method of camellia seeds, specifically includes following steps：
（1）Immersion：Camellia seeds are placed in clear water, the seed of floating is removed, clear water is just submerged seed, in 42 ~ 44 DEG C
Constant temperature stands 30 ~ 40 minutes, softens kind of a skin, obtains immersion seed；
（2）Enzymolysis：To cellulase is added in immersion seed, the mass fraction of cellulase is set to be 0.04 ~ 0.06%, it is placed in 31 ~
33 DEG C of constant temperature are digested 100 ~ 120 minutes, destroy seed coat structure, promote nutritional ingredient to enter, and accelerate germination, must digests and be planted
Son；
（3）Fermentation：Brewer's yeast is added to enzymolysis seed kind, makes volume fraction be 3 ~ 5%, fermented 28 ~ 30 hours in 16 ~ 18 DEG C,
The white granulated sugar of seed weight 5 ~ 7% is added, stirring adds acetic acid bacteria to dissolving completely, makes volume fraction be 4 ~ 6%, is placed in 34 ~ 36
DEG C ferment at constant temperature 8 ~ 10 hours, destruction kind skin and a dormancy material, antibacterial desinsection improves plumule activity, and filtering obtains fermentation seed；
（4）Vernalization：Fermentation seed is placed in germinating box, vernalization condition is gradually adjusted, vernalization 36 hours improves plumule resistance,
Accelerate plumule growth, shorten germinating time, obtain vernalization seed.
The step（4）Regulation vernalization condition, 1 ~ 7 hour, dark, temperature is 22 ~ 24 DEG C, humidity be 91 ~ 93%, 8 ~
23 hours, 200 ~ 300lx of illumination, temperature were 25 ~ 27 DEG C, humidity is 86 ~ 88%, 24 ~ 36 hours, 700 ~ 800lx of illumination, temperature
For 32 ~ 33 DEG C, humidity are 86 ~ 88%.
Described humidity, with sprinkling mass fraction for 0.16 ~ 0.18% chitosan solution keeps humidity, described shell gathers
Sugar juice, is that shitosan is dissolved in the acetum that volume fraction is 2 ~ 3%, is heated to 80 ~ 85 DEG C, and stirring to melting completely.
It is an advantage of the invention that：A kind of germination accelerating method of camellia seeds that the present invention is provided, method is simple, rapidly and efficiently,
The vernalization rate of camellia seeds reaches 98%, and germination time shorten to 4 ~ 5 days, survival rate to 96%, and income improves 11.3%；
Clear water immersion removes shrivelled seed, softens kind of a skin, and enzymolysis can further destroy the seed coat structure of camellia seeds, promote nutrition
Composition enters, and accelerates germination；Seed ferments through brewer's yeast and acetic acid bacteria, destruction kind skin and dormancy material, improves plumule
Resistance, improves survival rate；Seed is placed in germinating box, gradually adjusts vernalization condition, and sprinkling chitosan solution keeps humidity, increases
The application approach of shitosan, improves plumule resistance, accelerates plumule growth, shortens germinating time.
Specific embodiment
Below the present invention is illustrated with specific embodiment.
Embodiment 1
A kind of germination accelerating method of camellia seeds, specifically includes following steps：
（1）Immersion：Camellia seeds are placed in clear water, the seed of floating is removed, clear water is just submerged seed, in 42 ~ 44 DEG C
Constant temperature stands 30 minutes, softens kind of a skin, obtains immersion seed；
（2）Enzymolysis：To cellulase is added in immersion seed, make the mass fraction of cellulase be 0.04%, be placed in 31 DEG C of constant temperature
Enzymolysis 100 minutes, destroys seed coat structure, promotes nutritional ingredient to enter, and accelerates germination, obtains enzymolysis seed；
（3）Fermentation：Brewer's yeast is added to enzymolysis seed kind, makes volume fraction be 3%, fermented 28 hours in 16 DEG C, add seed
The white granulated sugar of weight 5%, stirring adds acetic acid bacteria to dissolving completely, makes volume fraction be 4%, is placed in 34 DEG C of ferment at constant temperature 8 small
When, destruction kind skin and dormancy material, antibacterial desinsection improve plumule activity, and filtering obtains fermentation seed；
（4）Vernalization：Fermentation seed is placed in germinating box, vernalization condition is gradually adjusted, vernalization 36 hours improves plumule resistance,
Accelerate plumule growth, shorten germinating time, obtain vernalization seed.
The step（4）Regulation vernalization condition, 1 ~ 7 hour, dark, temperature was 22 DEG C, humidity is 91%, 8 ~ 23 hours,
Illumination 200lx, temperature are 25 DEG C, humidity is 86%, and 24 ~ 36 hours, illumination 700lx, temperature were 32 DEG C, humidity is 86%.
Described humidity, humidity is kept with the chitosan solution that sprinkling mass fraction is 0.16%, and described shitosan is molten
Liquid, is that shitosan is dissolved in the acetum that volume fraction is 2%, is heated to 80 ~ 85 DEG C, and stirring to melting completely.
Embodiment 2
A kind of germination accelerating method of camellia seeds, specifically includes following steps：
（1）Immersion：Camellia seeds are placed in clear water, the seed of floating is removed, clear water is just submerged seed, in 43 DEG C of constant temperature
35 minutes are stood, softens kind of a skin, obtain immersion seed；
（2）Enzymolysis：To cellulase is added in immersion seed, make the mass fraction of cellulase be 0.05%, be placed in 32 DEG C of constant temperature
Enzymolysis 110 minutes, destroys seed coat structure, promotes nutritional ingredient to enter, and accelerates germination, obtains enzymolysis seed；
（3）Fermentation：Brewer's yeast is added to enzymolysis seed kind, makes volume fraction be 4%, fermented 29 hours in 17 DEG C, add seed
The white granulated sugar of weight 6%, stirring adds acetic acid bacteria to dissolving completely, makes volume fraction be 5%, is placed in 35 DEG C of ferment at constant temperature 9 small
When, destruction kind skin and dormancy material, antibacterial desinsection improve plumule activity, and filtering obtains fermentation seed；
（4）Vernalization：Fermentation seed is placed in germinating box, vernalization condition is gradually adjusted, vernalization 36 hours improves plumule resistance,
Accelerate plumule growth, shorten germinating time, obtain vernalization seed.
The step（4）Regulation vernalization condition, 1 ~ 7 hour, dark, temperature was 23 DEG C, humidity is 92%, 8 ~ 23 hours,
Illumination 250lx, temperature are 26 DEG C, humidity is 87%, and 24 ~ 36 hours, illumination 750lx, temperature were 32 DEG C, humidity is 87%.
Described humidity, humidity is kept with the chitosan solution that sprinkling mass fraction is 0.17%, and described shitosan is molten
Liquid, is that shitosan is dissolved in the acetum that volume fraction is 2%, is heated to 80 ~ 85 DEG C, and stirring to melting completely.
Embodiment 3
A kind of germination accelerating method of camellia seeds, specifically includes following steps：
（1）Immersion：Camellia seeds are placed in clear water, the seed of floating is removed, clear water is just submerged seed, in 44 DEG C of constant temperature
40 minutes are stood, softens kind of a skin, obtain immersion seed；
（2）Enzymolysis：To cellulase is added in immersion seed, make the mass fraction of cellulase be 0.06%, be placed in 33 DEG C of constant temperature
Enzymolysis 120 minutes, destroys seed coat structure, promotes nutritional ingredient to enter, and accelerates germination, obtains enzymolysis seed；
（3）Fermentation：Brewer's yeast is added to enzymolysis seed kind, makes volume fraction be 5%, fermented 30 hours in 18 DEG C, add seed
The white granulated sugar of weight 7%, stirring adds acetic acid bacteria to dissolving completely, makes volume fraction be 6%, is placed in 36 DEG C of ferment at constant temperature 10 small
When, destruction kind skin and dormancy material, antibacterial desinsection improve plumule activity, and filtering obtains fermentation seed；
（4）Vernalization：Fermentation seed is placed in germinating box, vernalization condition is gradually adjusted, vernalization 36 hours improves plumule resistance,
Accelerate plumule growth, shorten germinating time, obtain vernalization seed.
The step（4）Regulation vernalization condition, 1 ~ 7 hour, dark, temperature was 24 DEG C, humidity is 93%, 8 ~ 23 hours,
Illumination 300lx, temperature are 27 DEG C, humidity is 88%, and 24 ~ 36 hours, illumination 800lx, temperature were 33 DEG C, humidity is 88%.
Described humidity, humidity is kept with the chitosan solution that sprinkling mass fraction is 0.18%, and described shitosan is molten
Liquid, is that shitosan is dissolved in the acetum that volume fraction is 3%, is heated to 80 ~ 85 DEG C, and stirring to melting completely.
Comparative example 1
Removal step（1）, remaining method, with embodiment 1.
Comparative example 2
Removal step（2）, remaining method, with embodiment 1.
Comparative example 3
Removal step（3）, remaining method, with embodiment 1.
Comparative example 4
Step（4）The vernalization of dark standing is carried out, illumination and temperature are not changed, 26 ~ 28 DEG C of temperature holding is constant, and water spray regulation is wet
Degree, remaining method, with embodiment 1.
Comparative example 5
The germination accelerating method of existing camellia seeds.
The effect of the germination accelerating method of embodiment and comparative example camellia seeds：
The ripe camellia seeds 800 of the harvesting 2 years of same breed are randomly choosed, 8 groups are randomly divided into, every group 100, respectively
Embodiment group and comparative example group, carry out vernalization treatment, after vernalization with the method for embodiment and comparative example to the seed of camellia respectively
Planted in identical area, other management methods are identical, observed and recorded germination and the late growing stage situation of camellia seeds, it is real
The effect for applying the germination accelerating method of example and comparative example camellia seeds is shown in Table 1.
Table 1：The effect of the germination accelerating method of embodiment and comparative example camellia seeds
| Project | Vernalization rate/(%) | Germination time/(my god) | Survival rate/(%) |
| Embodiment 1 | 98 | 4~5 | 95 |
| Embodiment 2 | 96 | 4~5 | 96 |
| Embodiment 3 | 98 | 4~5 | 95 |
| Comparative example 1 | 85 | 4~5 | 91 |
| Comparative example 2 | 87 | 4~5 | 89 |
| Comparative example 3 | 83 | 2~3 | 90 |
| Comparative example 4 | 76 | 4~5 | 88 |
| Comparative example 5 | 68 | 16~17 | 84 |
From the results shown in Table 1, the germination accelerating method of the camellia seeds of embodiment, relative to comparative example, vernalization rate is high, vernalization
Time is short, and survival rate is high, illustrates the germination accelerating method of the camellia seeds of present invention offer and has preferable Germination effect.
Claims (3)
1. a kind of germination accelerating method of camellia seeds, it is characterised in that specifically include following steps：
（1）Immersion：Camellia seeds are placed in clear water, the seed of floating is removed, clear water is just submerged seed, in 42 ~ 44 DEG C
Constant temperature stands 30 ~ 40 minutes, obtains immersion seed；
（2）Enzymolysis：To cellulase is added in immersion seed, the mass fraction of cellulase is set to be 0.04 ~ 0.06%, it is placed in 31 ~
33 DEG C of constant temperature are digested 100 ~ 120 minutes, obtain enzymolysis seed；
（3）Fermentation：Brewer's yeast is added to enzymolysis seed kind, makes volume fraction be 3 ~ 5%, fermented 28 ~ 30 hours in 16 ~ 18 DEG C,
The white granulated sugar of seed weight 5 ~ 7% is added, stirring adds acetic acid bacteria to dissolving completely, makes volume fraction be 4 ~ 6%, is placed in 34 ~ 36
DEG C ferment at constant temperature 8 ~ 10 hours, filtering, obtains fermentation seed；
（4）Vernalization：Fermentation seed is placed in germinating box, vernalization condition is gradually adjusted, vernalization 36 hours obtains vernalization seed.
2. the germination accelerating method of camellia seeds according to claim 1, it is characterised in that the step（4）Regulation vernalization bar
Part, 1 ~ 7 hour, dark, temperature was 22 ~ 24 DEG C, humidity is 91 ~ 93%, 8 ~ 23 hours, and 200 ~ 300lx of illumination, temperature are 25 ~ 27
DEG C, humidity be 86 ~ 88%, 24 ~ 36 hours, 700 ~ 800lx of illumination, temperature be 32 ~ 33 DEG C, humidity be 86 ~ 88%.
3. the germination accelerating method of camellia seeds according to claim 1, it is characterised in that described humidity, with sprinkling quality point
Number keeps humidity for 0.16 ~ 0.18% chitosan solution, and described chitosan solution is that shitosan is dissolved in into volume fraction for 2
In ~ 3% acetum, 80 ~ 85 DEG C are heated to, stirring to melting completely.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201611041401.7A CN106688362A (en) | 2016-11-24 | 2016-11-24 | Germination hastening method for camellia japonica seeds |
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| CN201611041401.7A CN106688362A (en) | 2016-11-24 | 2016-11-24 | Germination hastening method for camellia japonica seeds |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107691083A (en) * | 2017-11-11 | 2018-02-16 | 安徽太阳花牧业有限公司 | A kind of seeding growing seedlings method of silk tree |
| CN108323426A (en) * | 2017-09-13 | 2018-07-27 | 界首市汉武家庭农场 | A kind of seeding growing seedlings method of azalea |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101898915A (en) * | 2009-05-26 | 2010-12-01 | 北京嘉博文生物科技有限公司 | Microbial agent suitable for crops in seedling stage and preparation method thereof |
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2016
- 2016-11-24 CN CN201611041401.7A patent/CN106688362A/en not_active Withdrawn
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101898915A (en) * | 2009-05-26 | 2010-12-01 | 北京嘉博文生物科技有限公司 | Microbial agent suitable for crops in seedling stage and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 孙洪波，张海清，赵梁军: "《园林植物繁殖原理与技术》", 30 June 2009, 中国农业科学技术出版社 * |
| 杨成利，史效玉，马春花，等: "《耐冬山茶种子萌发特性研究》", 《山东林业科技》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108323426A (en) * | 2017-09-13 | 2018-07-27 | 界首市汉武家庭农场 | A kind of seeding growing seedlings method of azalea |
| CN107691083A (en) * | 2017-11-11 | 2018-02-16 | 安徽太阳花牧业有限公司 | A kind of seeding growing seedlings method of silk tree |
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Application publication date: 20170524 |