CN106662576B - Plankton measuring device and its measurement method - Google Patents
Plankton measuring device and its measurement method Download PDFInfo
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- CN106662576B CN106662576B CN201580040878.0A CN201580040878A CN106662576B CN 106662576 B CN106662576 B CN 106662576B CN 201580040878 A CN201580040878 A CN 201580040878A CN 106662576 B CN106662576 B CN 106662576B
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- 230000005532 trapping Effects 0.000 claims abstract description 38
- 239000007921 spray Substances 0.000 claims abstract description 7
- 238000001914 filtration Methods 0.000 claims description 34
- 230000000694 effects Effects 0.000 claims description 14
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- 238000001514 detection method Methods 0.000 claims description 4
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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Abstract
The present invention relates to a kind of plankton measuring device and its measurement methods.Plankton measuring device according to an embodiment of the present invention, comprising: particle part flow arrangement, the spray nozzle part including the inflow part for passing air into and the side for being set to above-mentioned inflow part；Microorganism particle flow stream, the microorganism particle flow for making to have passed through said nozzle portion internal flow path in above-mentioned air；Driving device, for generating the flowing of mentioned microorganism particle；Capturing device is connected to mentioned microorganism particle flow stream, has the filter house for trapping mentioned microorganism particle；Shine measuring device, detects the amount or intensity of the light issued from the microorganism particle trapped by above-mentioned filter house；And sterilizing unit, it is set to the side of above-mentioned filter house, for sterilizing to above-mentioned filter house.
Description
Technical field
The present invention relates to a kind of measuring device of plankton and its measurement methods.
Background technique
In recent years, it with the appearance of bird flu, new type influenza etc., the problem of infection through air is becoming social concerns, surveys
The project of plankton (airborne microbial measurement) receives emphasis and treats in amount air, accordingly
Ground, biosensors market also sharp increase.
The method of plankton, there is cultivation, decoration method etc. in existing measurement air.Wherein, cultivation is by sample
The biomone to swim in gas is trapped in the solid or liquid surface for being suitble to proliferation, and trains under temperature and humidity conditions appropriate
After supporting the stipulated time, the method for trapping micro organism quantity is found out from the colony counts that surface occurs；After decoration method is dyeing
Utilize the method for fluorescence microscope.
In recent years, by utilizing ATP (atriphos, adenosine triphosphate) and fluorescein
(luciferin)/luciferase (luciferase) reacts and the ATP biloluminescence method of luminous principle, can will disappear from ATP
Except processing, ATP are extracted, the time needed for the series of steps until measurement luminous quantity foreshortens to 30 minutes or so, so as to
Realize sharp work.
However, by method as described above, can not real-time measurement be present in the plankton in air, need in addition
Include sampling process and pretreatment etc. a series of handworks, accordingly, there exist be not available this method to develop air
The limitation of middle plankton automatic measurement system.
Fig. 9 indicates the composition for the electrostatic (electric dust) precipitator being arranged on previous particle part flow arrangement.
Referring to Fig. 9, previous electrostatic (electric dust) precipitator 1, comprising: the entrapment plate 2 of two sides；And charging cable 3 (discharge electrode), setting
Between above-mentioned two sides entrapment plate.
When applying high voltage to above-mentioned charging cable 3, corona discharge can be generated, the ion generated at this time makes the rule in gas
Determine particle electrification.The particle of electrification passes through electric power to collecting electrode, i.e., to the movement of above-mentioned entrapment plate 2, so as to be captured.
Therefore, above-mentioned electrostatic (electric dust) precipitator 1 is it is understood that can trap the dust collect plant of regulation particle by electrostatic principle.On
State impurity or the plankton etc. that regulation particle may include dust etc..
In addition, previous plankton measuring device, comprising: above-mentioned electrostatic (electric dust) precipitator；And stick is collected, for collecting
State the plankton that entrapment plate traps.
In above-mentioned previous plankton measuring device, when upper by the driving plankton of above-mentioned electrostatic (electric dust) precipitator
When stating entrapment plate trapping, user by manual operation make collection stick contact with entrapment plate carry out plankton collection or
Person's sampling.
Moreover, making the plankton trapped be reacted and be shone with reagent, and it is micro- to measure to detect the light of sending
The concentration of biology.
So, it in previous plankton measuring device, needs in addition to prepare to collect stick, and need by using
The process that person collects the plankton for the plate trapping that is captured using stick is collected, accordingly, there exist consume a large amount of time and take
The problem of using.
Summary of the invention
Problem to be solved by the invention
The present invention is completed to solve the problems, such as described above, it is intended that providing one kind can survey rapidly
Measure the plankton measuring device and its measurement method of the plankton being present in gas phase.
The technical solution solved the problems, such as
Plankton measuring device according to an embodiment of the present invention, wherein include: particle part flow arrangement, including be used for
The spray nozzle part of the inflow part passed air into and the side for being set to above-mentioned inflow part；Microorganism particle flow stream, it is above-mentioned for making
The microorganism particle flow of said nozzle portion internal flow path is passed through in air；Driving device, for generating mentioned microorganism grain
The flowing of son；Capturing device is connected to mentioned microorganism particle flow stream, has the filtering for trapping mentioned microorganism particle
Portion；Shine measuring device, detects the amount or intensity of the light that the microorganism particle trapped from above-mentioned filter house issues；And it kills
Bacterium device is set to the side of above-mentioned filter house, for sterilizing to above-mentioned filter house.
In addition, further including shell, be set to the side of above-mentioned capturing device, for accommodate above-mentioned luminous measuring device and
Above-mentioned sterilizing unit.
In addition, further including sucting, it is formed in the inside of above-mentioned shell, it, will be above-mentioned by the driving of above-mentioned driving device
The flowing of microorganism particle is guided to above-mentioned filter house.
In addition, it is a feature of the present invention that above-mentioned luminous measuring device and above-mentioned sterilizing unit are set to above-mentioned sucking
The two sides in portion.
In addition, it is a feature of the present invention that above-mentioned capturing device includes filter cartridge, for accommodating above-mentioned filter house, and shape
At there is the filter hole that can be connected to mentioned microorganism particle flow stream, at least part of above-mentioned filter house passes through above-mentioned filter hole
Expose in outside.
In addition, it is a feature of the present invention that above-mentioned filter cartridge and above-mentioned filter house can rotate.
In addition, it is a feature of the present invention that above-mentioned filter cartridge rotation during, above-mentioned filter hole be configured in it is upper
Either state in sucting, above-mentioned acceptance part and above-mentioned sterilizing unit corresponding position.
In addition, it is a feature of the present invention that above-mentioned filter hole is configured in successively during the rotation of above-mentioned filter cartridge
Position corresponding with above-mentioned sucting, above-mentioned sterilizing unit and above-mentioned acceptance part.
In addition, it is a feature of the present invention that above-mentioned filter hole includes separated from each other multiple filter hole, above-mentioned multiple filterings
It is corresponding separated by a distance with above-mentioned sucting, above-mentioned sterilizing unit and above-mentioned acceptance part separated by a distance between hole.
In addition, it is a feature of the present invention that further including the control unit for controlling above-mentioned sterilizing unit, the control unit is above-mentioned micro-
Biomone is made above-mentioned sterilizing unit work before the trapping of above-mentioned filter house, removes the polluter in above-mentioned filter house.
In addition, it is a feature of the present invention that further including the control unit for controlling above-mentioned luminous measuring device, the control unit is upper
Microorganism particle is stated by before the trapping of above-mentioned filter house, so that above-mentioned luminous measuring device is carried out the first work, in mentioned microorganism
After particle is trapped by above-mentioned filter house, above-mentioned luminous measuring device is made to carry out the second work.
In addition, above-mentioned driving device includes air pump device.
In addition, further includes: air particles flow path, the air particles stream of the outer space for making to have passed through said nozzle portion
It is dynamic；And exhaust fan, for generating flowing in above-mentioned air particles flow path.
In addition, above-mentioned sterilizing unit, including ultraviolet radiation LED device or ion generator (ionizer).
In addition, above-mentioned luminous measuring device, comprising: acceptance part, for collecting light；And reflection apparatus for deivation, light is drawn
It is directed at above-mentioned acceptance part, and induces the total reflection of light perhaps the above-mentioned reflection apparatus for deivation of scattering includes film portion or coating portion.
In addition, further including display unit, the concentration of the microorganism detected in above-mentioned luminous measuring device is shown.
In addition, it is a feature of the present invention that when the microorganism concn for being shown in above-mentioned display unit is excessively high, microorganism is dense
The relevant information of degree is sent in the household appliances for purifying air.
According to another aspect of the present invention, the measurement method of plankton, comprising: execute first work in filtration drive portion
Make, sterilizing unit is made to be located at a region of filter house, and the step of making above-mentioned sterilizing unit work；Execute above-mentioned filtration drive portion
Second work, so that acceptance part is located at a region of above-mentioned filter house, execute above-mentioned acceptance part first work the step of；It executes
The third in above-mentioned filtration drive portion works, and the sucting for enabling microorganism particle to flow through is located at a region of above-mentioned filter house
Step；And driving device is driven, the microorganism particle in air is isolated, above-mentioned suction is passed through by isolated microorganism particle
Enter the step of portion is trapped by above-mentioned filter house.
In addition, further includes: be dissolved by the microorganism particle that above-mentioned filter house traps, dissolved microorganism particle and hair
The step of stimulative substance acts on；And the second work of above-mentioned acceptance part is executed, it detects according to above-mentioned dissolved microorganism particle
The step of with the luminous quantity of the effect of luminescent substance.
In addition, further including the second luminous quantity detected from the second work for executing above-mentioned acceptance part, subtract in execution
The first work of acceptance part is stated and the first luminous quantity for detecting, the step of luminous quantity to calculate microorganism.
Invention effect
Plankton measuring device and its measurement method according to an embodiment of the present invention, user catch without sampling manually
Collect the plankton on entrapment plate, the plankton in air is tied by virtual impactor (virtual impactor)
Structure can be automatically separated, and therefore, can be obtained particle branching process and be easy, the effect of required time reduction.
In addition, the filter house for the microorganism particle that can be split to trapping is sterilized, so as to prevent filter house
Pollution, as a result, measurement trap by filter house concentration of microorganism particles when, can reduce come from be present in above-mentioned filter house
On polluter influence.
In addition, the value for the measuring device measuring basis luminous quantity that shines is operated before microorganism particle is trapped by filter house,
And when calculating the value of luminous quantity for the microorganism particle becomeing trapped in later, it is contemplated that the value of said reference luminous quantity,
To have the advantages that the concentration of microorganism particle can be accurately calculated.
In addition, above-mentioned filter house can be made to be located at and be fitly configured at by driving filtration drive portion to keep filter house mobile
The side of sucting, acceptance part or sterilizing unit inside second shell, therefore, killing for filter house can be carried out continuously by having
The advantages of bacterium and measurement microorganism concn.
Also, since above-mentioned capturing device or filter house apply luminescent substance, the solubilising reagent of microorganism can be supplied
It is given to above-mentioned capturing device or filter house, therefore, has the effect of that luminous measurement process can be easily accomplished.
In addition, can efficiently separate the small main flow of particle according to virtual impactor structure and particle is relatively large auxiliary
(sub) is helped to flow.Moreover, the main flow side relatively small in the pressure loss, uses fan as driving portion, it is larger in the pressure loss
Auxiliary flow side, use low discharge pump as driving portion, as a result, with can prevent plankton device from becoming larger or becoming
The effect of weight.
In addition, being additionally provided with the luminous quantity that detects based on light emitting device to show the aobvious of the relevant information of microorganism concn
Show portion, when microorganism concn is setting concentration or more, Warning Sign can be shown on above-mentioned display unit, to improve
The convenience of user.
Detailed description of the invention
Fig. 1 is the perspective view for indicating the plankton measuring device structure of the embodiment of the present invention.
Fig. 2 is the cross-sectional view splitted along the I-I' line of Fig. 1.
Fig. 3 is the cross-sectional view splitted along the II-II' line of Fig. 1.
Fig. 4 is the schematic diagram for indicating the plankton measuring device internal structure of the embodiment of the present invention.
Fig. 5 is the schematic diagram for indicating the spray nozzle part structure of the embodiment of the present invention.
Fig. 6 is the block diagram for indicating the plankton measuring device structure of the embodiment of the present invention.
Fig. 7 is the flow chart for indicating the measurement method of plankton measuring device of the embodiment of the present invention.
Fig. 8 A to Fig. 8 E is the schematic diagram for indicating the effect of plankton measuring device of the embodiment of the present invention.
Fig. 9 is the schematic diagram for indicating to be arranged in the electrostatic (electric dust) precipitator structure of previous plankton measuring device.
Specific embodiment
Hereinafter, illustrating specific embodiments of the present invention referring to attached drawing.However, provided by thought of the invention is not limited to
Embodiment, those skilled in the art understand that can be readily apparent that other in the range of identical thought on the basis of inventive concept
Embodiment.
Fig. 1 is the schematic diagram for indicating the structure of plankton measuring device of the embodiment of the present invention, and Fig. 2 is along Fig. 1
The cross-sectional view splitted of I-I' line, Fig. 3 is the cross-sectional view splitted along the II-II' line of Fig. 1.
Referring to figs. 1 to Fig. 3, the plankton measuring device of the embodiment of the present invention, comprising: base portion 20；And multiple dresses
It sets, is set to the upside of above-mentioned base portion 20.
Above-mentioned multiple devices, comprising: particle part flow arrangement 100 sucks air, and separates micro- life of swimming in air
Object；And capturing device 200, the plankton isolated from above-mentioned particle part flow arrangement 100 are trapped by the capturing device 200.
Moreover, above-mentioned multiple devices, further includes: shine measuring device 300, is set to the side of above-mentioned capturing device 200,
Detect the amount or intensity of the light generated from above-mentioned plankton；And control device 400, with above-mentioned luminous measuring device
300 electrical connections.Above-mentioned luminous measuring device 300 includes acceptance part 320, for collecting light.
Above-mentioned control device 400, comprising: PCB410 is provided with multiple circuit blocks；And display unit 420, it is set to
It states on PCB410, shows the relevant information of plankton concentration.
Specifically, above-mentioned particle part flow arrangement 100, comprising: first shell 110 forms defined inner space；And
Upper side 112 is incorporated into the top of said first shell 110.Above-mentioned upper side 112 is formed with multiple gaps 121, as sucking
It is present in " the air inflow part " of the air of the outside of above-mentioned particle part flow arrangement 100.
The width in above-mentioned gap 121 can be within the scope of several millimeters (mm).Also, due to being formed in above-mentioned upper side 112
There are multiple above-mentioned gaps 121, so the resistance of the air flowed by above-mentioned gap 121, the i.e. inside in gap 121 and outside
Between pressure difference (differential pressure) it is small.Thereby, it is possible to substantially ensure through above-mentioned multiple inflows of gaps 121
The flow of air.
Said first shell 110 is internally provided with spray nozzle part 120, the air for making to flow into via above-mentioned gap 121
Pass through.That is, said nozzle portion 120 may be disposed at the inner space of said first shell 110.In addition, said nozzle portion 120 is upward
The downside for stating gap 121 separates and extends downwardly.
Said nozzle portion 120 may be provided with multiple, with the quantity in the above-mentioned multiple gaps 121 of correspondence, and can be spaced from each other
Configuration.As an example, as shown in Fig. 2, multiple spray nozzle parts 120 can be configured to horizontally be spaced from each other.
Said nozzle portion 120 includes internal flow path 125, so that via above-mentioned gap 121 into said first shell 110
Plankton flowing in the air that portion flows into.Above-mentioned internal flow path 125 forms the inner space in said nozzle portion 120.
It is formed with inlet portion 125a on above-mentioned internal flow path 125, to provide the one end in said nozzle portion 120, and makes to swim
Microorganism flows into above-mentioned internal flow path 125.As an example, above-mentioned inlet portion 125a is formed in the upper end of above-mentioned internal flow path 125
Portion.
The plankton particle in air flowed into via above-mentioned gap 121 is existed by above-mentioned inlet portion 125a flowing
Above-mentioned internal flow path 125, the air particles for having separated above-mentioned plankton particle flow in the outside of above-mentioned internal flow path 125
Space, and pass through air particles flow path 129.
Also, it is formed with outlet portion 125b on above-mentioned internal flow path 125, to provide the other end in said nozzle portion 120, and
The plankton particle for flowing through above-mentioned internal flow path 125 is discharged from said nozzle portion 120.As an example, above-mentioned outlet portion
125b is formed in the lower end of above-mentioned internal flow path 125.
The side of above-mentioned outlet portion 125b is formed with microorganism particle flow stream 127, so as to be arranged by above-mentioned outlet portion 125b
Plankton particle flow out.Above-mentioned air particles flow path 129 can be referred to as first flow path or main flow flow path, it will be upper
It states microorganism particle flow stream 127 and is referred to as second flow path or auxiliary flow flow path.
The lower end in said nozzle portion 120 is formed with demarcation plate 126, to separate above-mentioned air particles flow path 129 and micro- life
Object particle flow stream 127.The lower end in said nozzle portion 120, i.e. outlet portion 125b are incorporated on above-mentioned demarcation plate 126.In other words,
Above-mentioned outlet portion 125b can be formed in the inside of above-mentioned demarcation plate 126.
By above-mentioned demarcation plate 126, above-mentioned air particles flow path 129 and microorganism particle flow stream 127 are separated, so as to
Enough prevent the mixing of the particle of above-mentioned air particles flow path 129 and the particle of mentioned microorganism particle flow stream 127.
The side of said first shell 110 has second shell 130, and above-mentioned second shell 130 is to be arranged acceptance part 320
And sterilizing unit 330.Mentioned microorganism particle flow stream 127 prolongs from a lateral above-mentioned capturing device 200 of above-mentioned demarcation plate 126
It stretches, the inner space of above-mentioned second shell 130 forms at least part of mentioned microorganism particle flow stream 127.
Above-mentioned capturing device 200, is formed with: filter cartridge 210, for accommodating filter house 220；And multiple filter hole 215,
It is formed on above-mentioned filter cartridge 210.
At least part of above-mentioned filter cartridge 210 is inserted into the inside of above-mentioned second shell 130.As an example, above-mentioned second
Shell 130 can be configured to surround at least part of top and lower part of above-mentioned filter cartridge 210.
Above-mentioned filter cartridge 210 can have the section of substantially semi-circular shape.Above-mentioned multiple filter hole 215 can be along above-mentioned filtering
The edge of box 210 is spaced from each other configuration in a circumferential direction.And between above-mentioned multiple filter hole 215 separated by a distance can phase
Together.
Above-mentioned filter house 220 can be exposed to outside by above-mentioned multiple filter hole 215.In addition, flowing through mentioned microorganism grain
The microorganism particle on subflow road 127 is by any one filter hole 215 in above-mentioned multiple filter hole 215 by above-mentioned filter house 220
Trapping.
Above-mentioned filter house 220 can be set to the inside for being fixed on above-mentioned filter cartridge 210.In addition, above-mentioned filter cartridge 210 is arranged
For that can rotate.
The side of above-mentioned filter cartridge 210 is provided with filtration drive portion 250 (referring to Fig. 4), and above-mentioned filtration drive portion 250 uses
To provide rotary force to above-mentioned filter cartridge 210.Above-mentioned filtration drive portion 250 include can positive direction or opposite direction rotation horse
It reaches.As an example, said motor may include stepper motor.Rotary shaft 255 (referring to Fig. 4) is upward from above-mentioned filtration drive portion 250
State the extension of filter cartridge 210.
When driving above-mentioned filtration drive portion 250, above-mentioned rotary shaft 255 is rotated, and above-mentioned filter cartridge 210 passes through above-mentioned
Rotary shaft 255 can be rotated in direction clockwise or counter-clockwise.In addition, above-mentioned filter house 220 can be with above-mentioned filter cartridge
210 are rotated together.
When above-mentioned filter cartridge 210 and filter house 220 are at a position, a filter hole 215 and mentioned microorganism particle flux
Road 127 is connected to.Therefore, the microorganism particle for flowing through mentioned microorganism particle flow stream 127 is above-mentioned by an above-mentioned filter hole 215
Filter house 220 traps.At this point, trapping mentioned microorganism particle filter house 220 a region, can with pass through an above-mentioned filter hole
215 exposings are corresponding in the region of mentioned microorganism particle flow stream 127.
In addition, when above-mentioned filter cartridge 210 and filter house 220 rotate, other filter hole 215 and mentioned microorganism particle
Flow path 127 is connected to, because the position of an above-mentioned filter hole 215 is mobile, so as to be located at the acceptance part 320 of above-mentioned luminous measuring device
Or the side of sterilizing unit 330.
The side of above-mentioned capturing device 200 is provided with pump installation 360, as " driving device ", to make microorganism particle flux
It moves and is driven；And pump interconnecting piece 350, extend from above-mentioned second shell 130 to above-mentioned pump installation 360.Above-mentioned pump installation
360 may include air pump.
In the particle of mentioned microorganism particle flow stream 127, other than the microorganism particle trapped by above-mentioned filter house 220
Residual particles, as an example, air particles flow to above-mentioned pump installation 360 via said pump interconnecting piece 350.
The inside of above-mentioned second shell 130, including the sucting 310 being connected to said pump interconnecting piece 350.Above-mentioned sucting
310 are formed in the inside of above-mentioned second shell 130, and the attraction of above-mentioned pump installation 360 can work to it.As an example, on
Stating sucting 310 can be broken away by least part of above-mentioned second shell 130 or be formed by perforation.In addition, above-mentioned
Sucting 310 can be formed in the side of above-mentioned filter cartridge 210, may be formed at upside on attached drawing.
Therefore, when above-mentioned pump installation 360 is driven, air flowing is generated in mentioned microorganism particle flow stream 127, on
It states air flowing and passes through above-mentioned filter house 220 via above-mentioned sucting 310.In the process, microorganism particle can be by above-mentioned mistake
Filter portion 220 traps.Mentioned microorganism particle separated after air flowing, can be via said pump interconnecting piece 350 to said pump
Device 360 flows.
Said pump interconnecting piece 350 includes portion cyclone separator (cyclone) 351, and the flowing in the cyclone separator portion 351 is cut
Area is reduced from above-mentioned second shell 130 to above-mentioned pump installation 360.When air flowing is by above-mentioned cyclone separator portion 351,
Flowing velocity improves, so as to flow into above-mentioned pump installation 360.
Even if above-mentioned pump installation 360 ensures the effect of defined inhalation flow better than wind it is understood that generating the pressure loss
Fan the device of (fan).Therefore, particle flux is generated in mentioned microorganism particle flow stream 127 by using above-mentioned pump installation 360
It is dynamic, even if generating the pressure loss in said nozzle portion 120 or filter house 220, it can also improve suction efficiency.
In addition, since the amount of flow in mentioned microorganism particle flow stream 127 is smaller, the above-mentioned applicable low stream of air pump
Amount pump.Thereby, it is possible to prevent plankton measuring device from becoming larger or becoming the phenomenon of weight.
Above-mentioned luminous measuring device 300 includes the acceptance part 320 of microorganism particle, is located at above-mentioned 200 side of capturing device.
Specifically, above-mentioned acceptance part 320 can be located at the inside of above-mentioned second shell 130.Also, above-mentioned acceptance part 320
The side for being configured at above-mentioned sucting 310 can be separated.
Above-mentioned acceptance part 320 may include less expensive LED and CCD camera.As an example, above-mentioned LED can be indigo plant
Color LED.In addition, above-mentioned luminous measuring device 300 may be provided with acceptance part guiding device, above-mentioned acceptance part 320 is set
Side, for light is directed to above-mentioned acceptance parts 320.Also, above-mentioned acceptance part guiding device may include reflection apparatus for deivation, with
Induce the total reflection or scattering of light.As an example, above-mentioned reflection apparatus for deivation includes film portion or the painting with reflection function
Layer portion.
Between above-mentioned sucting 310 and above-mentioned acceptance part 320 separated by a distance, can in above-mentioned multiple filter hole 215
One filter hole is corresponding with the distance between other filter hole.Therefore, when an above-mentioned filter hole is configured right with above-mentioned sucting 310
When the position answered, other above-mentioned filter hole can be configured in position corresponding with above-mentioned acceptance part 320.
In other words, an above-mentioned filter hole is configured at the position that can be acted on via the mobilization force of above-mentioned sucting 310, above-mentioned
The luminous quantity that other filter hole are configured at the filter house 220 exposed via other above-mentioned filter hole can act on above-mentioned acceptance part
On 320 position.
After being trapped by the filter hole microorganism particle in above-mentioned multiple filter hole 215 by above-mentioned filter house 220, when
When filter cartridge 210 rotates, an above-mentioned filter hole be configured in above-mentioned acceptance part 320 it is opposite to position on.Above-mentioned acceptance part
The amount or intensity of the 320 detectable light issued from the microorganism particle of above-mentioned filter house 220.
Above-mentioned plankton measuring device further includes sterilizing unit 330, for the dirt to above-mentioned filter house 220 is present in
Dye substance is sterilized.Above-mentioned sterilizing unit 330 may include ultraviolet rays emitting apparatus or ion generator (ionizer).Make
As an example of, above-mentioned ultraviolet rays emitting apparatus includes ultraviolet radiation LED device (Ultra Violet-Light Emitting
Diode)。
Specifically, above-mentioned sterilizing unit 330 can be located at the inside of above-mentioned second shell 130.Also, above-mentioned sterilizing unit
330 can be spaced apart with above-mentioned sucting 310 in the other side of above-mentioned sucting 310.In other words, above-mentioned acceptance part 320, i.e. hair
Optical measurement instrument 300 and above-mentioned sterilizing unit 330 may be provided at the two sides of above-mentioned sucting 310.
Between above-mentioned sucting 310 and above-mentioned sterilizing unit 330 separated by a distance, can in above-mentioned multiple filter hole 215
A filter hole and the distance between other filter hole it is corresponding.Therefore, when an above-mentioned filter hole is configured at and above-mentioned sucting
When 310 corresponding position, other above-mentioned filter hole are configured in position corresponding with above-mentioned sterilizing unit 330.
In other words, an above-mentioned filter hole is configured at the position that can be acted on via the mobilization force of above-mentioned sucting 310, above-mentioned
Other filter hole, which are configured at above-mentioned sterilizing unit 330, can act on the filter house 220 exposed by other above-mentioned filter hole
Position.
Above-mentioned sucting 310 and acceptance part 320 and sterilizing unit 330, which can be spaced from each other, to be configured to and above-mentioned multiple filterings
The configuration shape in hole 215 is corresponding.As an example, above-mentioned multiple filter hole 215 can be separated along the circumference of above-mentioned filter cartridge 210
Configuration, above-mentioned sucting 310, acceptance part 320 and sterilizing unit 330 can be with each filter hole 215 of above-mentioned multiple filter hole 215
Accordingly configure.
Above-mentioned plankton measuring device 10 further include: solvent supplying apparatus 370, Xiang Shangshu filter house 220 supply molten
Solve reagent；And supply line 375, prolong from above-mentioned solvent supplying apparatus 370 to an above-mentioned filter hole 215 or filter house 220
It stretches.
Above-mentioned solubilising reagent (lysis reagent) can be regarded as being swum for dissolving by what above-mentioned filter house 220 trapped
The solvent of microbial cell (or cell wall).When the cell of above-mentioned plankton particle is reacted with above-mentioned solubilising reagent,
ATP can be extracted.
In addition, luminescent substance can be coated on above-mentioned filter house 220.Above-mentioned luminescent substance is it is understood that with by above-mentioned
The ATP (Adenosine Triphosphate, atriphos) for the microorganism particle that solubilising reagent extracts reacts and generates light
Substance.
Above-mentioned luminescent substance includes fluorescein (luciferin) and luciferase (luciferase).Above-mentioned fluorescein
It is present in the intracellular ATP activation of dissolution and becomes active fluoro element, above-mentioned active fluoro element is as the glimmering of Luminescence Enzyme
It is oxidized under the action of light element enzyme and becomes oxyluciferin, to convert luminous energy for chemical energy and shine.
The inside of said first shell 110 is formed with air particles flow path 129, flows in above-mentioned air particles flow path 129
There are the smaller particless in the entrance side separation in said nozzle portion 120, air particles flow wherein as an example.Above-mentioned air grain
The particle on subflow road 129 is less than the particle of mentioned microorganism particle flow stream 127.However, the flowing of above-mentioned air particles flow path 129
Amount can be greater than the amount of flow of mentioned microorganism particle flow stream 127.
Above-mentioned air particles flow path 129 is isolated from mentioned microorganism particle flow stream 127 by above-mentioned demarcation plate 126 and to row
150 side of fan extends.
Driving device of the said exhaust fan 150 as the flowing for generating above-mentioned air particles flow path 129, as one
Example, can be accommodated in the inside of blower-casting 155.Said fans shell 155 is configured at the lower part of said first shell 110.
Also, said exhaust fan 150 is interpreted as, and pressure loss hour can ensure sufficient flow compared with above-mentioned air pump
Device.Therefore, by the way that exhaust fan 150 is arranged on the small flow path of the pressure loss in such as above-mentioned air particles flow path 129, have
The effect of sufficient air particles flowing (main flow) can be generated.
Fig. 4 is the schematic diagram for indicating the internal structure of plankton measuring device of the embodiment of the present invention, and Fig. 5 is to indicate
The schematic diagram of the spray nozzle part structure of the embodiment of the present invention.Referring to 4 and Fig. 5, the plankton of the embodiment of the present invention is measured
The effect of device carries out simple illustration.
When driving above-mentioned pump installation 360 and exhaust fan 150, it is present in outside above-mentioned plankton measuring device 10
Air (A of Fig. 5) by multiple gaps 121 of above-mentioned upper surface portion 112 to flowing into inside said first shell 110.
Air can increase its flow velocity by narrow flow path cross sectional area during passing through above-mentioned multiple gap 121.Pass through
Particle biggish plankton particle in the air in above-mentioned multiple gaps 121, via the inlet portion in said nozzle portion 120
125a flows into above-mentioned internal flow path 125 (C of Fig. 5).
Also, above-mentioned plankton particle after the discharge of above-mentioned internal flow path 125, is flowed by above-mentioned outlet portion 125b
In mentioned microorganism particle flow stream 127.
On the contrary, having passed through the air particles that particle is relatively small in the air in above-mentioned multiple gaps 121, because of its direction of advance quilt
Change and the flowing of internal flow path 125 cannot be stated upwards, but flows (B of Fig. 5) along the outer space in said nozzle portion 120.
Also, above-mentioned air particles flow through above-mentioned air particles flow path 129 and fan 150 by said exhaust.
To sum up, for air during the nozzle by narrow sectional area flows, relatively large plankton particle is logical
It crosses above-mentioned inlet portion 125a and flows into above-mentioned internal flow path 125, relatively small air particles pass through above-mentioned gap 121 and inlet portion
The space spaced apart of 125a, changes flow direction (stream line) and flows.
Particle flow dividing structure as described above, can be referred to as virtual impactor (virtual impactor) structure, this implementation
Example is applicable in above-mentioned virtual impactor structure, can easily shunt plankton particle and air particles.
The plankton particle of mentioned microorganism particle flow stream 127 is flowed through, Xiang Shangshu capturing device 200 flows, and passes through
By a filter hole 215 of above-mentioned sucting 310 and filter cartridge 210, can be trapped by a region of filter house 220.
After such trapping implementation Process setting time, supplied from above-mentioned solvent supplying apparatus 370 to above-mentioned filter house 220 molten
Solve reagent.
It is dissolved by microorganism particle that above-mentioned filter house 220 traps by above-mentioned solubilising reagent after extracting ATP, it can
It is reacted with the luminescent substance being coated on above-mentioned filter house 220.
In addition, rotating above-mentioned filter cartridge 210 by the driving in above-mentioned filtration drive portion 250, make as a result, above-mentioned
One filter hole 215 is located towards the position of above-mentioned acceptance part 320.Also, above-mentioned acceptance part 320 is able to detect from by above-mentioned filtering
The amount or intensity for the light that the microorganism particle that portion 220 traps issues.Here, above-mentioned light can be in the ATP of mentioned microorganism particle
It is generated with during luminescent substance reaction.
So, by the driving in filtration drive portion 250, the one of the filter house 220 of microorganism particle will can be trapped
Region is mobile, makes it towards above-mentioned acceptance part 320.As a result, filter cartridge 210 and filter house are arranged in rotatable manner
220, have the effect of that microorganism trapping and luminescence process can be automatically performed.
In addition, above-mentioned sterilizing unit 330 can be started to upper before microorganism particle is by the trapping of above-mentioned filter house 220
Filter house 220 is stated to be sterilized.
Also, before microorganism particle is by the trapping of above-mentioned filter house 220, above-mentioned acceptance part 320 can be started to detect
The luminous quantity of above-mentioned filter house 220.Luminous quantity at this time provides benchmark letter to luminous quantity when hereafter trapping microorganism particle
Breath, therefore luminous quantity at this time can be referred to as " benchmark luminous quantity ".
Fig. 6 is the block diagram for indicating the plankton measuring device structure of the embodiment of the present invention.
Referring to Fig. 6, the plankton measuring device 10 of the embodiment of the present invention, comprising: pump installation 360 makes micro- life of swimming
Object particle generates flowing；And exhaust fan 150, so that air particles is generated flowing.
Also, above-mentioned plankton measuring device 10 further include: filtration drive portion 250 makes filter cartridge 210 and filtering
Portion 220 rotates；And solvent supplying apparatus 370, for supplying solubilising reagent to above-mentioned filter house 220.
Above-mentioned plankton measuring device 10 includes display unit 420, is swum with display by what above-mentioned filter house 220 trapped
The relevant information of concentration of microorganism particles.Above-mentioned display unit 420 may include lighting device, according to above-mentioned plankton particle
Concentration value show different colors.
As an example, above-mentioned lighting device can include: the first illumination portion, when the concentration of above-mentioned plankton particle is low
Display green；Second illumination portion shows yellow when concentration is substantially median；And third illumination portion, when concentration is high with
Red display.
As another example, above-mentioned first illumination portion to third illumination portion can be set to an illumination portion.
Above-mentioned plankton measuring device 10, comprising: acceptance part 320 detects the micro- life trapped by above-mentioned filter house 220
The luminous quantity of object particle；And timer 460, the trapping process of accumulative mentioned microorganism particle and above-mentioned solubilising reagent supplied
Journey passes through the time.
The information detected by above-mentioned acceptance part 320 or timer 460, may pass to control unit 450, based on upper
State the information transmitted, above-mentioned control unit 450 can control above-mentioned pump installation 360, exhaust fan 150, filtration drive portion 250, molten
The work of agent feeding device 370 and display unit 420.
Above-mentioned plankton measuring device 10 further includes sterilizing unit 330, is present in above-mentioned filter house 220 for removing
Polluter.By making above-mentioned sterilizing unit 330 work, the polluter that removal is present on above-mentioned filter house 220, by
This, can prevent above-mentioned polluter from influencing luminous phenomenon.As a result, it is possible to accurately detect and calculate the dense of microorganism
Degree.
Also, above-mentioned plankton measuring device 10 further includes storage unit 470, and above-mentioned storage unit 470 is to store hair
The relevant information of work of optical measurement instrument, i.e. above-mentioned acceptance part 320.Specifically, microorganism can be performed in above-mentioned acceptance part 320
Particle be captured before first work and microorganism particle be captured after second work.
Above-mentioned first work is the work for detecting the luminous quantity of the light based on 200 periphery of capturing device, it will be appreciated that is
Detect the work of said reference luminous quantity.The information of benchmark luminous quantity about above-mentioned first work, can be stored in above-mentioned storage
In portion 470.
Also, when computationally stating the luminous quantity detected after the second work, it may be considered that shine about said reference
The information of amount.Said reference luminous quantity can be referred to as " the first luminous quantity ", and the luminous quantity detected after above-mentioned second work can claim
Make " the second luminous quantity ".As an example, the concentration value of the microorganism trapped by filter house can be based on subtracting from above-mentioned second luminous quantity
The value of said reference luminous quantity is gone to calculate.
Fig. 7 is the flow chart for indicating the measurement method of plankton measuring device of the embodiment of the present invention, Fig. 8 A to figure
8E is the schematic diagram for indicating the effect of plankton measuring device of the embodiment of the present invention.
In order to facilitate understanding, each attached drawing of Fig. 8 A to Fig. 8 E diagram is respectively indicated the filter cartridge 210 or so of semi-circular shape
Extend, and relatively indicates above-mentioned sucting 310, acceptance part 320 and sterilizing unit 330 in the side of above-mentioned filter cartridge 210
Position form.
In addition, the form of Fig. 8 A to 8E, indicates during measuring plankton, with the rotation of above-mentioned filter cartridge 210
Turn, what the position of above-mentioned trapping filter hole 251a changed relative to above-mentioned sucting 310, acceptance part 320 and sterilizing unit 330
Form.
Referring to Fig. 7, when the power supply of above-mentioned plankton measuring device 10 connects (ON), above-mentioned filtration drive portion 250 is held
Row first works.First work in above-mentioned filtration drive portion 250 is the work for rotating in the opposite direction the first set angle, it will be appreciated that
To make the filter hole 215a (referring to Fig. 8 A) in the region for opening the filter house 220 for trapping microorganism to the one of sterilizing unit 300
The mobile work in side.Above-mentioned filter hole 215a can be referred to as " trapping filter hole ".
Here, above-mentioned opposite direction can be corresponding with the direction that above-mentioned filter cartridge 210 moves to the left on the basis of Fig. 8 A.
In addition, above-mentioned first set angle is it is understood that above-mentioned filter cartridge 210 can rotate a filter hole to above-mentioned one
The angle of the distance between other closest filter hole of filter hole (separated by a distance)." the first set angle of opposite direction will be somebody's turn to do
Rotation " can be referred to as " -1 rotation " (S12).
Fig. 8 A is the basic configuration form for indicating above-mentioned plankton measuring device 10, that is, indicates above-mentioned plankton
The form when the power is on of measuring device 10.At this point, above-mentioned sucting 310 is located at the trapping filter hole of above-mentioned filter cartridge 210
The side of 215a, above-mentioned sterilizing unit are located at the side of other filter hole.Also, above-mentioned acceptance part 320 can be located at multiple above-mentioned
The outside of filter hole.
In addition, above-mentioned filter cartridge 210 is rotated and is configured after executing first work in above-mentioned filtration drive portion 250
At as shown in Figure 8 B, above-mentioned sterilizing unit 330 is located at the side of the trapping filter hole 215a of above-mentioned filter cartridge 210.That is, above-mentioned kill
Bacterium device 330 is configured at can be by (ginseng on position that a region of the above-mentioned trapping filter hole 215a to filter house 220 is sterilized
According to Fig. 8 B).Above-mentioned sterilizing unit 330 can be to an area illumination light source (S13) of above-mentioned filter house 220.
After so that above-mentioned sterilizing unit 330 is worked, above-mentioned filtration drive portion 250 executes the second work.Above-mentioned filtration drive
Second work in portion 250 is the work for being rotated to positive direction the second set angle, it will be appreciated that is by above-mentioned trapping filter hole 215a
The work mobile to the side of acceptance part 320.
Here, above-mentioned positive direction can be corresponding with the direction that above-mentioned filter cartridge 210 moves to the right on the basis of Fig. 8 B.
In addition, above-mentioned second set angle is it is understood that above-mentioned filter cartridge 210 can rotate above-mentioned 2 separated by a distance times
The angle of distance.Being somebody's turn to do " the second set angle of positive direction rotates " can be referred to as "+2 rotation " (S14).
After the second work for executing above-mentioned filtration drive portion 250, above-mentioned filter cartridge 210 is configured to as shown in Figure 8 C, above-mentioned
Acceptance part 320 is located at the side of above-mentioned trapping filter hole 215a.That is, be configured at can be by above-mentioned trapping for above-mentioned acceptance part 320
Filter hole 215a detects the position of the luminous quantity in a region of filter house 220 (referring to Fig. 8 C).In addition, in multiple filter hole its
The side of his filter hole is configured with sucting 310, may be configured with sterilizing unit 330 in the side of other filter hole.This is because,
Above-mentioned sucting 310, acceptance part 320 and sterilizing unit 330 be each spaced apart by distance respectively with multiple above-mentioned filter hole every
It opens apart from corresponding reason.
Above-mentioned acceptance part 320, the measuring device that shines implement the first work, detect the luminous quantity of above-mentioned filter house 220.
By the first operation detection of above-mentioned acceptance part 320 to luminous quantity be, microorganism particle be captured before upper
The luminous quantity being able to detect that substantially in filter house 220 is stated, there is " benchmark luminous quantity (the first luminous quantity) " value.In addition, above-mentioned
The relevant information of benchmark luminous quantity can be stored in above-mentioned storage unit 470 (S15).
After first work of above-mentioned acceptance part 320, above-mentioned filtration drive portion 250 executes third work.Above-mentioned filtering is driven
The third work in dynamic portion 250 is the work for rotating in the opposite direction third set angle, it will be appreciated that make above-mentioned trapping filter hole
Work 215a mobile to the side of sucting 310.
Here, above-mentioned opposite direction can be corresponding with the left side moving direction to above-mentioned filter cartridge 210 on the basis of Fig. 8 C.
In addition, above-mentioned third set angle can be regarded as that above-mentioned filter cartridge 210 can be made to rotate above-mentioned angle separated by a distance
Degree." the third set angle of opposite direction rotates ", which will be somebody's turn to do, can be referred to as " -1 rotation " (S16).
After the third work for executing above-mentioned filtration drive portion 250, above-mentioned filter cartridge 210 is configured to as in fig. 8d, above-mentioned
Sucting 310 is configured at the side of above-mentioned trapping filter hole 215a.That is, above-mentioned sucting 310 be configured at microorganism particle can
The position flowed by above-mentioned sucting 310 and trapping filter hole 215a to a region of filter house 220.
Also, make said exhaust fan 150 and pump installation 360 work, generate to above-mentioned exhaust fan 150 main flow and
To the auxiliary flow of above-mentioned pump installation 360.When making said exhaust fan 150 and pump installation 360 work, above-mentioned plankton
The outside air of measuring device 10 is flowed into said first shell 110 by multiple above-mentioned gaps 121.
Plankton particle and air by the virtual impactor structure inside said first shell 110, in air
Particle is separated and respectively flows through microorganism particle flow stream 127 and air particles flow path 129.In addition, as in fig. 8d, flowing through
The particle of mentioned microorganism particle flow stream 127 is by above-mentioned sucting 310 and trapping filter hole 215a, by above-mentioned filter house
220 trappings (S17).
This implementable first setting time of trapping process.Pass through time, above-mentioned control by the way that above-mentioned timer 460 is accumulative
Portion 450 identifies whether to have passed through the first setting time (S18).
After above-mentioned first setting time, the driving of said exhaust fan 150 and pump installation 360 stops.Then, make
Above-mentioned solvent supplying apparatus 370 works, and Xiang Shangshu filter house 220 supplies solubilising reagent.In above-mentioned second setting time, Xiang Shangshu
Filter house 220 supplies above-mentioned solubilising reagent, after above-mentioned second setting time, in the work of above-mentioned solvent supplying apparatus 370
Only.
Above-mentioned solubilising reagent dissolution extracts ATP by the microorganism particle that above-mentioned filter house 220 traps, the ATP being extracted
It is reacted with the luminescent substance being coated on above-mentioned filter house 220, issues defined light (S19, S20).
Above-mentioned filtration drive portion 250 executes the 4th work.4th work in above-mentioned filtration drive portion 250 is revolved to positive direction
Turn the work of the 4th set angle, it will be appreciated that make above-mentioned trapping filter hole 215a to the movement of the side of above-mentioned acceptance part 320
Work.
Here, above-mentioned positive direction can be corresponding with the direction that above-mentioned filter cartridge 210 moves to the right on the basis of Fig. 8 D.
In addition, above-mentioned 4th set angle can be regarded as that above-mentioned filter cartridge 210 can be made to rotate above-mentioned angle separated by a distance
Degree.Being somebody's turn to do " the 4th set angle of positive direction rotates " can be referred to as "+1 rotation " (S21).
After the 4th work for executing above-mentioned filtration drive portion 250, above-mentioned filter cartridge 210 is configured to as illustrated in fig. 8e, above-mentioned
Acceptance part 320 is configured at the side of above-mentioned trapping filter hole 215a.That is, above-mentioned acceptance part 320, which is configured at, to be caught by above-mentioned
Collection filter hole 215a detection has trapped the position of a region luminous quantity of the filter house 220 of microorganism particle (referring to Fig. 8 E).On
Acceptance part 320, the measuring device that shines are stated by executing the second work, the luminous quantity of above-mentioned filter house 220 is detected or it is strong
Degree.
Above-mentioned luminous quantity or its intensity can be proportional to microorganism concn.That is, when above-mentioned luminous quantity or its intensity are big
When, it is identified as because mentioned microorganism concentration is proportional to greatly, when above-mentioned luminous quantity or its small intensity, because of above-mentioned micro- life
Object concentration is proportional to and is identified as small.
By the second operation detection of above-mentioned acceptance part 320 to luminous quantity be that microorganism particle can after being captured
In the luminous quantity that above-mentioned filter house 220 detects, it will be appreciated that be the luminous quantity (second for reflecting the concentration of mentioned microorganism particle
Luminous quantity) (S22).
Above-mentioned control unit 450 can be by luminous quantity (microorganism corresponding with the microorganism concn trapped by above-mentioned filter house 220
Luminous quantity), it is determined as subtracting the value of above-mentioned first luminous quantity from above-mentioned second luminous quantity.
Above-mentioned control unit 450 can be based on mentioned microorganism luminous quantity, and the relevant information of microorganism concn is shown in
It states on display unit 420.As an example, according to microorganism concn, the mutually different photograph of color can be activated on above-mentioned display unit 420
Bright portion (S23).
So, due to can automatically and continuously complete microorganism particle trapping and luminous measuring process, can
It is easily accomplished the measurement process of plankton.Also, it is being shown due to that can show the relevant information of microorganism concn
In portion, therefore, have the effect of that user can easily confirm plankton concentration.
In addition, may be provided with household appliances with the linkage of above-mentioned plankton measuring device, for air cleaning.When
Be shown in the plankton on above-mentioned display unit concentration it is high when, i.e. when the pollution level of plankton is serious, above-mentioned family
Electricity can be driven.Above-mentioned household appliances may include air purifier, air interchanger or air conditioner.That is, above-mentioned plankton
Measuring device can guide the work of above-mentioned household appliances to the relevant information of above-mentioned household appliances transmission microorganism concn.
In addition, due to that can be sterilized before microorganism particle is trapped by filter house to above-mentioned filter house, it can
Prevent the miscalculation of concentration of microorganism particles caused by the polluter because of filter house.
Also, the luminous quantity of the filter house before microorganism particle is captured is detected, and is reflected in microorganism particle
In the calculating of concentration, therefore, the measurement of mentioned microorganism particle concentration can be more accurately completed.
Industrial applicibility
According to an embodiment of the invention, the filter house for the microorganism particle that can be split to trapping is sterilized, therefore,
The pollution of filter house can be prevented, as a result, when measuring the concentration of the microorganism particle trapped by filter house, can reduce presence
In the influence of the polluter of above-mentioned filter house, therefore, industrial applicibility is significant.
Claims (14)
1. a kind of plankton measuring device, wherein include:
Particle part flow arrangement, the spray nozzle part including the inflow part for passing air into and the side for being set to above-mentioned inflow part；
Microorganism particle flow stream, the microorganism particle flow for making to have passed through said nozzle portion internal flow path in above-mentioned air；
Driving device, for generating the flowing of mentioned microorganism particle；
Capturing device is connected to mentioned microorganism particle flow stream, has: filter cartridge, and being formed with can be with mentioned microorganism particle
It the filter hole of fluid communication and can rotate and filter house, be contained in the inside of above-mentioned filter cartridge, trap mentioned microorganism grain
Son and can by above-mentioned filter hole expose in outside；
Shine measuring device, detects the amount or intensity of the light issued from the microorganism particle trapped by above-mentioned filter house；With
And
Sterilizing unit is set to the side of above-mentioned filter house, for sterilizing to above-mentioned filter house；And
Shell for accommodating above-mentioned luminous measuring device and above-mentioned sterilizing unit, and is formed with mentioned microorganism particle
Flowing is guided to the sucting of above-mentioned filter house,
Above-mentioned shell is set to the side of above-mentioned capturing device, and above-mentioned sucting is formed in the inside of above-mentioned shell,
During above-mentioned filter cartridge and above-mentioned filter house rotate, above-mentioned filter hole can be configured at above-mentioned sucting,
The corresponding position of any of above-mentioned sterilizing unit and above-mentioned luminous measuring device.
2. plankton measuring device according to claim 1, which is characterized in that
Above-mentioned luminous measuring device and above-mentioned sterilizing unit are set to the two sides of above-mentioned sucting.
3. plankton measuring device according to claim 1, which is characterized in that
Above-mentioned filter house can be rotated together with above-mentioned filter cartridge.
4. plankton measuring device according to claim 1, which is characterized in that
During above-mentioned filter cartridge and above-mentioned filter house rotate, above-mentioned filter hole can be configured at successively with above-mentioned sucking
Portion, above-mentioned sterilizing unit and the corresponding position of above-mentioned luminous measuring device.
5. plankton measuring device according to claim 1, which is characterized in that
Above-mentioned filter hole includes separated from each other multiple filter hole, between above-mentioned multiple filter hole separated by a distance with above-mentioned sucking
The distance that portion, above-mentioned sterilizing unit and above-mentioned luminous measuring device separate is corresponding.
6. plankton measuring device according to claim 1, which is characterized in that
It further include the control unit for controlling above-mentioned sterilizing unit,
Above-mentioned control unit makes above-mentioned sterilizing unit work before mentioned microorganism particle is by the trapping of above-mentioned filter house, removes above-mentioned
Polluter in filter house.
7. plankton measuring device according to claim 1, which is characterized in that
It further include the control unit for controlling above-mentioned luminous measuring device,
Above-mentioned control unit makes above-mentioned luminous measuring device carry out first before mentioned microorganism particle is by the trapping of above-mentioned filter house
Work makes above-mentioned luminous measuring device carry out the second work after mentioned microorganism particle is by the trapping of above-mentioned filter house.
8. plankton measuring device according to claim 1, wherein
Above-mentioned driving device includes air pump device.
9. plankton measuring device according to claim 1, wherein further include:
Air particles flow path, the air particles flowing of the outer space for making to have passed through said nozzle portion；And
Exhaust fan, for generating flowing in above-mentioned air particles flow path.
10. plankton measuring device according to claim 1, wherein
Above-mentioned sterilizing unit includes ultraviolet radiation LED device or ion generator.
11. plankton measuring device according to claim 1, wherein
Above-mentioned luminous measuring device includes:
Acceptance part, for collecting light；And
Apparatus for deivation is reflected, light is directed to above-mentioned acceptance parts, and induce the total reflection or scattering of light,
Above-mentioned reflection apparatus for deivation includes film portion or coating portion.
12. plankton measuring device according to claim 1, wherein further include:
Display unit is shown in the concentration of the microorganism detected in above-mentioned luminous measuring device.
13. plankton measuring device according to claim 12, which is characterized in that
When the microorganism concn for being shown in above-mentioned display unit is high, microorganism concn relevant information is sent to and is used to purify air
Household appliances.
14. a kind of plankton measurement method, wherein include:
The first work for executing filtration drive portion keeps filter house mobile, so that sterilizing unit is located at a region of filter house, and make
The step of stating sterilizing unit work；
The second work for executing above-mentioned filtration drive portion keeps filter house mobile, and acceptance part is made to be located at a region of above-mentioned filter house,
The step of executing the first work of above-mentioned acceptance part；
The third work for executing above-mentioned filtration drive portion keeps filter house mobile, and the sucting for enabling microorganism particle to flow through is located at
The step of one region of above-mentioned filter house；And
Driving device is driven, the microorganism particle in air is isolated, above-mentioned sucting is passed through by isolated microorganism particle
The step of being trapped by above-mentioned filter house；
It is dissolved by the microorganism particle that above-mentioned filter house traps, the step of dissolved microorganism particle and luminescent substance effect
Suddenly；
Execute the second work of above-mentioned acceptance part, the hair of detection basis dissolved mentioned microorganism particle and luminescent substance effect
The step of light quantity；And
The second luminous quantity detected from the second work for executing above-mentioned acceptance part, subtracts the first work for executing above-mentioned acceptance part
The step of the first luminous quantity made and detected, luminous quantity to calculate microorganism.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020140095700A KR102221557B1 (en) | 2014-07-28 | 2014-07-28 | Airborne microbial measurement apparatus and measurement method |
| KR10-2014-0095700 | 2014-07-28 | ||
| PCT/KR2015/006908 WO2016017950A1 (en) | 2014-07-28 | 2015-07-06 | Airborne micro-organism measurement apparatus and measurement method therefor |
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| CN106662576A CN106662576A (en) | 2017-05-10 |
| CN106662576B true CN106662576B (en) | 2019-08-02 |
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| CN201580040878.0A Expired - Fee Related CN106662576B (en) | 2014-07-28 | 2015-07-06 | Plankton measuring device and its measurement method |
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| Country | Link |
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| KR (1) | KR102221557B1 (en) |
| CN (1) | CN106662576B (en) |
| WO (1) | WO2016017950A1 (en) |
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| KR102337848B1 (en) * | 2017-04-13 | 2021-12-10 | 엘지전자 주식회사 | Apparatus for measuring airborne microbial, measuring method using the same and air conditioning device having the same |
| WO2019193878A1 (en) * | 2018-04-06 | 2019-10-10 | パナソニックＩｐマネジメント株式会社 | Pathogen detection device and pathogen detection method |
| KR102207043B1 (en) * | 2019-05-17 | 2021-01-25 | 주식회사 더웨이브톡 | Apparatus for detecting airborne mcicrobes |
| JP7037842B2 (en) | 2018-05-18 | 2022-03-17 | ザ ウェーブ トーク， インコーポレイテッド | Optical detection system |
| US11391659B2 (en) | 2018-05-18 | 2022-07-19 | The Wave Talk, Inc. | Optical detecting system |
| CN108998367A (en) * | 2018-08-30 | 2018-12-14 | 上海海事大学 | A kind of portable microbial aerosol sampling apparatus can be used for high-flux sequence |
| KR102528012B1 (en) * | 2019-05-17 | 2023-05-03 | 주식회사 더웨이브톡 | Apparatus for detecting airborne mcicrobes |
| US20220373436A1 (en) * | 2020-02-04 | 2022-11-24 | Steve Naumovski | A system and method for detecting airborne pathogens |
| KR102421489B1 (en) * | 2020-03-20 | 2022-07-15 | 주식회사 파이퀀트 | Hazardous ingredient measurement device and hazardous ingredient analysis system using the same |
| CN111855365A (en) * | 2020-06-19 | 2020-10-30 | 东洋工业(广东)有限公司 | Microorganism detection device |
| KR102514890B1 (en) | 2020-11-24 | 2023-03-29 | 한국과학기술연구원 | Method and Apparatus for bio-aerosol analyzing |
| KR20230102642A (en) | 2021-12-30 | 2023-07-07 | 경희대학교 산학협력단 | Investigation of bacterial and fungal communities in indoor and outdoor air of classrooms by 16S rRNA gene and ITS region sequencing |
| TWI832353B (en) * | 2022-07-28 | 2024-02-11 | 研能科技股份有限公司 | Method for detecting, locating and cleaning indoor microbiotics |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1813058A (en) * | 2003-06-04 | 2006-08-02 | 贝尔坦技术有限公司 | Device for collecting and separating particles and microorganisms present in ambient air |
| CN100519731C (en) * | 2006-12-27 | 2009-07-29 | 清华大学深圳研究生院 | Method and dedicated device for enriching air microorganism |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH03112495A (en) * | 1989-09-28 | 1991-05-14 | Japan Organo Co Ltd | Detection of microorganism floating in air |
| BRPI0214680B1 (en) * | 2001-12-06 | 2018-09-18 | Biocontrol Systems Inc | instrument for use in monitoring a product, ingredient, environment or process, instrument for detecting light emission from a sample and method for monitoring a sample of a product, ingredient, process or environment |
| ES2324952T3 (en) * | 2004-07-02 | 2009-08-20 | Promega Corporation | COMPOSITIONS AND PROCESSES FOR THE EXTRACTION AND DETECTION OF MICROBIAL ATP. |
| KR101148308B1 (en) * | 2007-11-29 | 2012-05-25 | 연세대학교 산학협력단 | Microorganism meter apparatus |
| KR101667060B1 (en) * | 2011-01-26 | 2016-10-18 | 연세대학교 산학협력단 | airborne microbial measurement apparatus and measurement method using the microorganism dissolution system and ATP-luminescence |
| KR102199814B1 (en) * | 2014-02-27 | 2021-01-08 | 엘지전자 주식회사 | Airborne microbial measurement apparatus and measurement method |
| KR102203201B1 (en) * | 2014-02-27 | 2021-01-14 | 엘지전자 주식회사 | An air cleaning system and a method controlling the same |
-
2014
- 2014-07-28 KR KR1020140095700A patent/KR102221557B1/en active IP Right Grant
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- 2015-07-06 WO PCT/KR2015/006908 patent/WO2016017950A1/en active Application Filing
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1813058A (en) * | 2003-06-04 | 2006-08-02 | 贝尔坦技术有限公司 | Device for collecting and separating particles and microorganisms present in ambient air |
| CN100519731C (en) * | 2006-12-27 | 2009-07-29 | 清华大学深圳研究生院 | Method and dedicated device for enriching air microorganism |
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| Publication number | Publication date |
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| KR20160013646A (en) | 2016-02-05 |
| WO2016017950A1 (en) | 2016-02-04 |
| CN106662576A (en) | 2017-05-10 |
| KR102221557B1 (en) | 2021-03-02 |
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