CN106579456A - Novel material composition capable of regulating activity of intestinal flora and preparation method of novel material composition - Google Patents
Novel material composition capable of regulating activity of intestinal flora and preparation method of novel material composition Download PDFInfo
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- CN106579456A CN106579456A CN201611153825.2A CN201611153825A CN106579456A CN 106579456 A CN106579456 A CN 106579456A CN 201611153825 A CN201611153825 A CN 201611153825A CN 106579456 A CN106579456 A CN 106579456A
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- 239000000203 mixture Substances 0.000 title claims abstract description 55
- 230000000968 intestinal effect Effects 0.000 title claims abstract description 40
- 230000001105 regulatory effect Effects 0.000 title claims abstract description 35
- 238000002360 preparation method Methods 0.000 title claims abstract description 22
- 239000000463 material Substances 0.000 title abstract description 8
- 241000894006 Bacteria Species 0.000 claims abstract description 31
- 235000001270 Allium sibiricum Nutrition 0.000 claims abstract description 28
- 241001646823 Phlomis mongolica Species 0.000 claims abstract description 27
- 240000002900 Arthrospira platensis Species 0.000 claims abstract description 19
- 235000016425 Arthrospira platensis Nutrition 0.000 claims abstract description 19
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 19
- 229940082787 spirulina Drugs 0.000 claims abstract description 19
- 150000007524 organic acids Chemical class 0.000 claims abstract description 13
- 239000011812 mixed powder Substances 0.000 claims description 52
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 32
- 210000000582 semen Anatomy 0.000 claims description 31
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Chemical compound CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 claims description 30
- 230000001580 bacterial effect Effects 0.000 claims description 30
- 239000002023 wood Substances 0.000 claims description 29
- 241000192031 Ruminococcus Species 0.000 claims description 19
- 230000001954 sterilising effect Effects 0.000 claims description 18
- 238000004659 sterilization and disinfection Methods 0.000 claims description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 18
- 239000004310 lactic acid Substances 0.000 claims description 16
- 235000014655 lactic acid Nutrition 0.000 claims description 16
- 241000607598 Vibrio Species 0.000 claims description 14
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 13
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 13
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 13
- 241000194020 Streptococcus thermophilus Species 0.000 claims description 12
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 12
- 239000000843 powder Substances 0.000 claims description 11
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 10
- 244000063299 Bacillus subtilis Species 0.000 claims description 9
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 9
- 238000010009 beating Methods 0.000 claims description 9
- 238000005119 centrifugation Methods 0.000 claims description 9
- 239000003795 chemical substances by application Substances 0.000 claims description 9
- 238000001035 drying Methods 0.000 claims description 9
- 239000002244 precipitate Substances 0.000 claims description 9
- 239000002002 slurry Substances 0.000 claims description 9
- 239000006228 supernatant Substances 0.000 claims description 9
- 241000186016 Bifidobacterium bifidum Species 0.000 claims description 8
- 241000186000 Bifidobacterium Species 0.000 claims description 5
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 claims description 4
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 4
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 claims description 4
- 239000001630 malic acid Substances 0.000 claims description 4
- 235000011090 malic acid Nutrition 0.000 claims description 4
- 235000011201 Ginkgo Nutrition 0.000 claims description 3
- 244000194101 Ginkgo biloba Species 0.000 claims description 3
- 235000008100 Ginkgo biloba Nutrition 0.000 claims description 3
- VTAJIXDZFCRWBR-UHFFFAOYSA-N Licoricesaponin B2 Natural products C1C(C2C(C3(CCC4(C)CCC(C)(CC4C3=CC2)C(O)=O)C)(C)CC2)(C)C2C(C)(C)CC1OC1OC(C(O)=O)C(O)C(O)C1OC1OC(C(O)=O)C(O)C(O)C1O VTAJIXDZFCRWBR-UHFFFAOYSA-N 0.000 claims description 3
- LPLVUJXQOOQHMX-UHFFFAOYSA-N glycyrrhetinic acid glycoside Natural products C1CC(C2C(C3(CCC4(C)CCC(C)(CC4C3=CC2=O)C(O)=O)C)(C)CC2)(C)C2C(C)(C)C1OC1OC(C(O)=O)C(O)C(O)C1OC1OC(C(O)=O)C(O)C(O)C1O LPLVUJXQOOQHMX-UHFFFAOYSA-N 0.000 claims description 3
- 239000001685 glycyrrhizic acid Substances 0.000 claims description 3
- 229960004949 glycyrrhizic acid Drugs 0.000 claims description 3
- UYRUBYNTXSDKQT-UHFFFAOYSA-N glycyrrhizic acid Natural products CC1(C)C(CCC2(C)C1CCC3(C)C2C(=O)C=C4C5CC(C)(CCC5(C)CCC34C)C(=O)O)OC6OC(C(O)C(O)C6OC7OC(O)C(O)C(O)C7C(=O)O)C(=O)O UYRUBYNTXSDKQT-UHFFFAOYSA-N 0.000 claims description 3
- 235000019410 glycyrrhizin Nutrition 0.000 claims description 3
- LPLVUJXQOOQHMX-QWBHMCJMSA-N glycyrrhizinic acid Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@H](O[C@@H]1O[C@@H]1C([C@H]2[C@]([C@@H]3[C@@]([C@@]4(CC[C@@]5(C)CC[C@@](C)(C[C@H]5C4=CC3=O)C(O)=O)C)(C)CC2)(C)CC1)(C)C)C(O)=O)[C@@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O LPLVUJXQOOQHMX-QWBHMCJMSA-N 0.000 claims description 3
- JXOHGGNKMLTUBP-HSUXUTPPSA-N shikimic acid Chemical compound O[C@@H]1CC(C(O)=O)=C[C@@H](O)[C@H]1O JXOHGGNKMLTUBP-HSUXUTPPSA-N 0.000 claims description 3
- JXOHGGNKMLTUBP-JKUQZMGJSA-N shikimic acid Natural products O[C@@H]1CC(C(O)=O)=C[C@H](O)[C@@H]1O JXOHGGNKMLTUBP-JKUQZMGJSA-N 0.000 claims description 3
- TWJNQYPJQDRXPH-UHFFFAOYSA-N 2-cyanobenzohydrazide Chemical compound NNC(=O)C1=CC=CC=C1C#N TWJNQYPJQDRXPH-UHFFFAOYSA-N 0.000 claims description 2
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 claims description 2
- TUNFSRHWOTWDNC-UHFFFAOYSA-N Myristic acid Natural products CCCCCCCCCCCCCC(O)=O TUNFSRHWOTWDNC-UHFFFAOYSA-N 0.000 claims description 2
- 235000021360 Myristic acid Nutrition 0.000 claims description 2
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 claims description 2
- 235000015165 citric acid Nutrition 0.000 claims description 2
- 239000011975 tartaric acid Substances 0.000 claims description 2
- 235000002906 tartaric acid Nutrition 0.000 claims description 2
- 241000196324 Embryophyta Species 0.000 claims 1
- 210000000481 breast Anatomy 0.000 claims 1
- 208000019790 abdominal distention Diseases 0.000 abstract description 7
- 206010012735 Diarrhoea Diseases 0.000 abstract description 6
- 206010010774 Constipation Diseases 0.000 abstract description 5
- 230000009286 beneficial effect Effects 0.000 abstract description 4
- 206010047700 Vomiting Diseases 0.000 abstract description 3
- 206010061428 decreased appetite Diseases 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 3
- 206010028813 Nausea Diseases 0.000 abstract description 2
- 230000008693 nausea Effects 0.000 abstract description 2
- 208000004998 Abdominal Pain Diseases 0.000 abstract 1
- 241001280436 Allium schoenoprasum Species 0.000 abstract 1
- GXCLVBGFBYZDAG-UHFFFAOYSA-N N-[2-(1H-indol-3-yl)ethyl]-N-methylprop-2-en-1-amine Chemical compound CN(CCC1=CNC2=C1C=CC=C2)CC=C GXCLVBGFBYZDAG-UHFFFAOYSA-N 0.000 abstract 1
- 238000009776 industrial production Methods 0.000 abstract 1
- 208000028774 intestinal disease Diseases 0.000 abstract 1
- 230000035876 healing Effects 0.000 description 14
- 201000010099 disease Diseases 0.000 description 11
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 11
- 238000000855 fermentation Methods 0.000 description 7
- 230000004151 fermentation Effects 0.000 description 7
- 208000024891 symptom Diseases 0.000 description 7
- 208000002193 Pain Diseases 0.000 description 6
- 210000001015 abdomen Anatomy 0.000 description 5
- 230000013872 defecation Effects 0.000 description 5
- 210000003608 fece Anatomy 0.000 description 5
- 210000001035 gastrointestinal tract Anatomy 0.000 description 4
- 208000035415 Reinfection Diseases 0.000 description 3
- 241000933856 bacterium 8 Species 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 206010000087 Abdominal pain upper Diseases 0.000 description 2
- 206010019233 Headaches Diseases 0.000 description 2
- 230000003187 abdominal effect Effects 0.000 description 2
- 230000004596 appetite loss Effects 0.000 description 2
- 208000002173 dizziness Diseases 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 206010016766 flatulence Diseases 0.000 description 2
- 230000002496 gastric effect Effects 0.000 description 2
- 231100000869 headache Toxicity 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 208000019017 loss of appetite Diseases 0.000 description 2
- 235000021266 loss of appetite Nutrition 0.000 description 2
- 210000003205 muscle Anatomy 0.000 description 2
- 230000008855 peristalsis Effects 0.000 description 2
- 235000018291 probiotics Nutrition 0.000 description 2
- TUNFSRHWOTWDNC-HKGQFRNVSA-N tetradecanoic acid Chemical compound CCCCCCCCCCCCC[14C](O)=O TUNFSRHWOTWDNC-HKGQFRNVSA-N 0.000 description 2
- 230000008673 vomiting Effects 0.000 description 2
- 244000025254 Cannabis sativa Species 0.000 description 1
- 241001478240 Coccus Species 0.000 description 1
- 108010048312 Combizym Proteins 0.000 description 1
- 244000182067 Fraxinus ornus Species 0.000 description 1
- 235000002917 Fraxinus ornus Nutrition 0.000 description 1
- 239000009636 Huang Qi Substances 0.000 description 1
- 229920001202 Inulin Polymers 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 241001619461 Poria <basidiomycete fungus> Species 0.000 description 1
- 239000010231 banlangen Substances 0.000 description 1
- YBHILYKTIRIUTE-UHFFFAOYSA-N berberine Chemical compound C1=C2CC[N+]3=CC4=C(OC)C(OC)=CC=C4C=C3C2=CC2=C1OCO2 YBHILYKTIRIUTE-UHFFFAOYSA-N 0.000 description 1
- 229940093265 berberine Drugs 0.000 description 1
- QISXPYZVZJBNDM-UHFFFAOYSA-N berberine Natural products COc1ccc2C=C3N(Cc2c1OC)C=Cc4cc5OCOc5cc34 QISXPYZVZJBNDM-UHFFFAOYSA-N 0.000 description 1
- 229930182830 galactose Natural products 0.000 description 1
- JYJIGFIDKWBXDU-MNNPPOADSA-N inulin Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@]1(OC[C@]2(OC[C@]3(OC[C@]4(OC[C@]5(OC[C@]6(OC[C@]7(OC[C@]8(OC[C@]9(OC[C@]%10(OC[C@]%11(OC[C@]%12(OC[C@]%13(OC[C@]%14(OC[C@]%15(OC[C@]%16(OC[C@]%17(OC[C@]%18(OC[C@]%19(OC[C@]%20(OC[C@]%21(OC[C@]%22(OC[C@]%23(OC[C@]%24(OC[C@]%25(OC[C@]%26(OC[C@]%27(OC[C@]%28(OC[C@]%29(OC[C@]%30(OC[C@]%31(OC[C@]%32(OC[C@]%33(OC[C@]%34(OC[C@]%35(OC[C@]%36(O[C@@H]%37[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O%37)O)[C@H]([C@H](O)[C@@H](CO)O%36)O)[C@H]([C@H](O)[C@@H](CO)O%35)O)[C@H]([C@H](O)[C@@H](CO)O%34)O)[C@H]([C@H](O)[C@@H](CO)O%33)O)[C@H]([C@H](O)[C@@H](CO)O%32)O)[C@H]([C@H](O)[C@@H](CO)O%31)O)[C@H]([C@H](O)[C@@H](CO)O%30)O)[C@H]([C@H](O)[C@@H](CO)O%29)O)[C@H]([C@H](O)[C@@H](CO)O%28)O)[C@H]([C@H](O)[C@@H](CO)O%27)O)[C@H]([C@H](O)[C@@H](CO)O%26)O)[C@H]([C@H](O)[C@@H](CO)O%25)O)[C@H]([C@H](O)[C@@H](CO)O%24)O)[C@H]([C@H](O)[C@@H](CO)O%23)O)[C@H]([C@H](O)[C@@H](CO)O%22)O)[C@H]([C@H](O)[C@@H](CO)O%21)O)[C@H]([C@H](O)[C@@H](CO)O%20)O)[C@H]([C@H](O)[C@@H](CO)O%19)O)[C@H]([C@H](O)[C@@H](CO)O%18)O)[C@H]([C@H](O)[C@@H](CO)O%17)O)[C@H]([C@H](O)[C@@H](CO)O%16)O)[C@H]([C@H](O)[C@@H](CO)O%15)O)[C@H]([C@H](O)[C@@H](CO)O%14)O)[C@H]([C@H](O)[C@@H](CO)O%13)O)[C@H]([C@H](O)[C@@H](CO)O%12)O)[C@H]([C@H](O)[C@@H](CO)O%11)O)[C@H]([C@H](O)[C@@H](CO)O%10)O)[C@H]([C@H](O)[C@@H](CO)O9)O)[C@H]([C@H](O)[C@@H](CO)O8)O)[C@H]([C@H](O)[C@@H](CO)O7)O)[C@H]([C@H](O)[C@@H](CO)O6)O)[C@H]([C@H](O)[C@@H](CO)O5)O)[C@H]([C@H](O)[C@@H](CO)O4)O)[C@H]([C@H](O)[C@@H](CO)O3)O)[C@H]([C@H](O)[C@@H](CO)O2)O)[C@@H](O)[C@H](O)[C@@H](CO)O1 JYJIGFIDKWBXDU-MNNPPOADSA-N 0.000 description 1
- 229940029339 inulin Drugs 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000010271 massa medicata fermentata Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- XOFYZVNMUHMLCC-ZPOLXVRWSA-N prednisone Chemical compound O=C1C=C[C@]2(C)[C@H]3C(=O)C[C@](C)([C@@](CC4)(O)C(=O)CO)[C@@H]4[C@@H]3CCC2=C1 XOFYZVNMUHMLCC-ZPOLXVRWSA-N 0.000 description 1
- 229960004618 prednisone Drugs 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a novel material composition capable of regulating activity of intestinal flora and a preparation method of the novel material composition. The novel material composition consists of the following components in parts by weight: 15-30 parts of strain, 10-20 parts of small molecular organic acid, 1-2 parts of fruit oligose, 1-10 parts of Radix Et Caulis Acanthopanacis Senticosi, 3-10 parts of spirulina, 10-20 parts of roasted malt, 1-2 parts of Phlomis mongolica Turcz., 1-2 parts of Allium schoenoprasum and 3-10 parts of nutlet. The novel material composition capable of regulating the activity of the intestinal flora can regulate the intestinal flora, growth of beneficial bacteria is promoted, growth of harmful bacteria is inhibited, and repeatedly relapsed intestinal diseases such as diarrhea, abdominal pain, abdominal distention, inappetence, nausea, emesis, constipation and the like can be cured. The preparation method is simple and practicable, and is low in preparation cost and suitable for large-scale industrial production.
Description
Technical field
The invention belongs to new material technology development field, and in particular to a kind of new material combination of regulating intestinal canal bacterial activity
Thing and preparation method thereof.
Background technology
Contain substantial amounts of probioticss and harmful bacteria in intestinal, symbiosis together, has when the harmful bacteria growing in intestinal exceedes
The growth of beneficial bacteria, will cause intestinal tract disease, common intestinal tract disease to have constipation, diarrhoea, stomachache, abdominal distention, and inappetence is disliked
The heart, vomiting etc., it is all improper with the regulation of intestinal microbial population relevant.These diseases are treated typically using dried yeast tablets, Combizym, prednisone
Deng western medicine, advantage is instant effect, but, there is side effect to human body, disease easily recurs repeatedly.
Patent 201610654551.9 discloses a kind of compositionss of regulating intestinal canal microorganism species and preparation method thereof.By
The component composition of following mass fraction:Bacillus bifiduss 5-8 parts, lactic acid bacteria 5-8 parts, inulin 8-10 parts, oligofructose 8-10 parts are low
Poly- galactose 8-10 parts, manna oligosaccharide 8-10 parts, berberine 8-10 parts, Radix Scutellariae 6-9 parts, Radix Isatidis 6-9 parts, Radix Astragali 6-9 parts,
Poria 6-9 parts, Herba Schizonepetae 5-8 parts, Radix Saposhnikoviae 5-8 parts, Rhizoma Atractylodis 3-4 parts, Rhizoma Alismatis 3-4 parts, Radix Puerariae 3-4 parts, Massa Medicata Fermentata 3-4 parts, Fructus Crataegi 3-4
Part, Cortex Magnoliae Officinalis 3-4 part, Fructus Forsythiae 3-4 parts.Said composition component as a kind of health product to intestinal beneficial, it can however not as medicine
Use, treat the intestinal tract disease that intractable recurs repeatedly.
The content of the invention
It is an object of the invention to provide a kind of green wood feed composition of regulating intestinal canal bacterial activity.
The present invention also aims to provide the preparation method of the green wood feed composition of above-mentioned regulating intestinal canal bacterial activity.
A kind of green wood feed composition of regulating intestinal canal bacterial activity, is made up of the component of following parts by weight:Strain 15-30
Part, small molecular organic acid 10-20 parts, fruit oligose 1-2 parts, Radix Et Caulis Acanthopanacis Senticosi 1-10 parts, spirulina 3-10 parts, Fructus Hordei Germinatus (parched) 10-20 parts, string
Bell grass 1-2 parts, Chive 1-2 parts, kernel 3-10 parts.
The strain includes lactic acid bacteria mycopowder, bacillus subtilises mycopowder, butanoic acid vibrio mycopowder, Bifidobacteria powder, tumor
Stomach coccus mycopowder, Lactobacillus plantarum mycopowder, one or more in streptococcus thermophiluss mycopowder.
The lactic acid bacteria mycopowder contains lactic acid bacteria 1-8X108Individual/g;Bacillus subtilises mycopowder contains bacillus subtilises
1-8X108Individual/g;Butanoic acid vibrio mycopowder contains butanoic acid vibrio 1-8X108Individual/g;Bifidobacteria powder contains bacillus bifiduss 1-
8X108Individual/g;Ruminococcuses mycopowder contains Ruminococcuses 1-8X108Individual/g;Lactobacillus plantarum mycopowder contains Lactobacillus plantarum 1-
8X108Individual/g;Streptococcus thermophiluss mycopowder contains streptococcus thermophiluss 1-8X108Individual/g.
The small molecular organic acid be malic acid, shikimic acid, citric acid, tartaric acid, glycyrrhizic acid, the one kind in myristic acid
Or more than one.
The kernel be Semen arachidis hypogaeae, Semen Helianthi, Semen Juglandiss, Semen Castaneae, Semen Armeniacae Amarum, Semen Cucurbitae, Semen Citrulli, Fructus anacardii, Semen Pini, Fructus Pistaciae Verae,
Semen Ginkgo, one or more in Semen Nelumbinis.
The preparation method of the green wood feed composition of above-mentioned regulating intestinal canal bacterial activity, is carried out in accordance with the following steps:
(1) by Fructus Hordei Germinatus (parched), kernel is crushed, and is crossed 100-300 mesh sieves, is made mixed-powder A;
(2) by Radix Et Caulis Acanthopanacis Senticosi, Phlomis mongolica Turcz., Chive are mixed with water, beating, filter bits, and serosity is obtained, and add brown sugar, are surpassed
High-temperature instantaneous sterilization, adds yeast bacteria agent to carry out 24-36 hours of fermenting, and centrifugation goes supernatant, drying precipitate that mixed powder is obtained
Last B;
(3) by strain, small molecular organic acid, fruit oligose, spirulina mix homogeneously make mixed-powder C;
(4) by mixed-powder A, mixed powder B and mixed-powder C mix homogeneously are preserved under the conditions of -40 DEG C -4 DEG C.
The Radix Et Caulis Acanthopanacis Senticosi, Phlomis mongolica Turcz., the mass ratio that Chive is mixed with water are 1:(1-10).
The quality that the brown sugar is added accounts for the 0.5-3% of grind slurries quality.
The ultra high temperature short time sterilization is to sterilize the 6-10 seconds under the conditions of 137-145 DEG C.
Beneficial effects of the present invention:The green wood feed composition of the regulating intestinal canal bacterial activity of the present invention, can regulating intestinal canal bacterium
Group, promotes the growth of probioticss and suppresses the growth of harmful bacteria, can cure the intestinal tract disease of repeatability recurrence as suffered from diarrhoea, stomachache,
Abdominal distention, inappetence, nausea, vomiting, constipation etc..Its preparation method is simple, and preparation cost is low, is adapted to heavy industrialization
Production.
Specific embodiment
With reference to specific embodiment, the present invention will be further described.
Embodiment 1
A kind of green wood feed composition of regulating intestinal canal bacterial activity, is made up of the component of following parts by weight:Lactic acid bacteria bacterium
8 parts of powder, 8 parts of butanoic acid vibrio mycopowder, 8 parts of Ruminococcuses mycopowder, 15 parts of malic acid, 1.5 parts of fruit oligose, 5 parts of Radix Et Caulis Acanthopanacis Senticosi, spirulina
6 parts, 15 parts of Fructus Hordei Germinatus (parched), 1.5 parts of Phlomis mongolica Turcz., 1.5 parts of Chive, 7 parts of Semen Cucurbitae;The lactic acid bacteria mycopowder contains lactic acid bacteria
3X108Individual/g;Butanoic acid vibrio mycopowder contains butanoic acid vibrio 4X108Individual/g;Ruminococcuses mycopowder contains Ruminococcuses 4X108Individual/g.
The preparation method of the green wood feed composition of above-mentioned regulating intestinal canal bacterial activity, is carried out in accordance with the following steps:
(1) by Fructus Hordei Germinatus (parched), Semen Cucurbitae is crushed, and is crossed 200 mesh sieves, is made mixed-powder A;
(2) by Radix Et Caulis Acanthopanacis Senticosi, Phlomis mongolica Turcz., Chive are mixed with water, beating, filter bits, and serosity is obtained, and add brown sugar, are surpassed
High-temperature instantaneous sterilization, adds yeast bacteria agent to carry out fermentation 30 hours, and centrifugation goes supernatant, drying precipitate that mixed powder B is obtained;
The Radix Et Caulis Acanthopanacis Senticosi, Phlomis mongolica Turcz., the mass ratio that Chive is mixed with water are 1:5.The quality that the brown sugar is added accounts for grind slurries quality
1%;The ultra high temperature short time sterilization is to sterilize 7 seconds under the conditions of 138 DEG C;
(3) by strain, small molecular organic acid, fruit oligose, spirulina mix homogeneously make mixed-powder C;
(4) by mixed-powder A, mixed powder B and mixed-powder C mix homogeneously are preserved under the conditions of -20 DEG C.
100 diarrhea patients are chosen, disease recurs repeatedly.Symptom is:Frequently, each excrement amount is few and after having pain in the abdomen for awareness of defecation
Reinfection, has pus and blood in feces, arrange post-defecatory pain and do not alleviate.Take the compositionss of the present embodiment 15 days, remission person 65;
Take 30 days, remission person 75, cure 30;Take 60 days, remission person 86, healing person 80.Healing person 2 years
Interior recurrence 7.
Embodiment 2
A kind of green wood feed composition of regulating intestinal canal bacterial activity, is made up of the component of following parts by weight:Hay spore
7 parts of bacillus mycopowder, 8 parts of Lactobacillus plantarum mycopowder, 5 parts of Ruminococcuses mycopowder, 10 parts of citric acid, 1 part of fruit oligose, 1 part of Radix Et Caulis Acanthopanacis Senticosi,
3 parts of spirulina, 10 parts of Fructus Hordei Germinatus (parched), 1 part of Phlomis mongolica Turcz., 1 part of Chive, 3 parts of Semen Juglandiss;Bacillus subtilises mycopowder contains hay spore
Bacillus 2X108Individual/g;Lactobacillus plantarum mycopowder contains Lactobacillus plantarum 7X108Individual/g;Ruminococcuses mycopowder contains Ruminococcuses
7X108Individual/g.
The preparation method of the green wood feed composition of above-mentioned regulating intestinal canal bacterial activity, is carried out in accordance with the following steps:
(1) by Fructus Hordei Germinatus (parched), kernel is crushed, and is crossed 100 mesh sieves, is made mixed-powder A;
(2) by Radix Et Caulis Acanthopanacis Senticosi, Phlomis mongolica Turcz., Chive are mixed with water, beating, filter bits, and serosity is obtained, and add brown sugar, are surpassed
High-temperature instantaneous sterilization, adds yeast bacteria agent to carry out fermentation 24 hours, and centrifugation goes supernatant, drying precipitate that mixed powder B is obtained;
The Radix Et Caulis Acanthopanacis Senticosi, Phlomis mongolica Turcz., the mass ratio that Chive is mixed with water are 1:2;The quality that the brown sugar is added accounts for grind slurries quality
0.5%;The ultra high temperature short time sterilization is to sterilize 10 seconds under the conditions of 137 DEG C;
(3) by strain, small molecular organic acid, fruit oligose, spirulina mix homogeneously make mixed-powder C;
(4) by mixed-powder A, mixed powder B and mixed-powder C mix homogeneously are preserved under the conditions of -40 DEG C.
100 constipation patients are chosen, disease recurs repeatedly.Symptom is:Stool is reduced, and in one week, times of defecation is less than
2~3 times, or 2~3 talent stool 1 time, feces amount is few and dry and hard, with loss of appetite, abdominal distention, lower abdomen be uncomfortable, aerofluxuss are more or
There are dizziness, headache.Take the compositionss of the present embodiment 15 days, remission person 55;Take 30 days, remission person 88,
Cure 60;Take 60 days, remission person 90, healing person 88.Healing person recurred 3 in 2 years.
Embodiment 3
A kind of green wood feed composition of regulating intestinal canal bacterial activity, is made up of the component of following parts by weight:Bacillus bifiduss
10 parts of mycopowder, 10 parts of streptococcus thermophiluss mycopowder, 8 parts of Lactobacillus plantarum mycopowder, 20 parts of myristic acid, 2 parts of fruit oligose, Radix Et Caulis Acanthopanacis Senticosi 10
Part, 10 parts of spirulina, 20 parts of Fructus Hordei Germinatus (parched), 2 parts of Phlomis mongolica Turcz., 2 parts of Chive, 5 parts of Fructus anacardii, 5 parts of Semen Nelumbinis;Bifidobacteria powder contains
Bacillus bifiduss 3X108Individual/g;Streptococcus thermophiluss mycopowder contains streptococcus thermophiluss 5X108Individual/g.
The preparation method of the green wood feed composition of above-mentioned regulating intestinal canal bacterial activity, is carried out in accordance with the following steps:
(1) by Fructus Hordei Germinatus (parched), kernel is crushed, and is crossed 300 mesh sieves, is made mixed-powder A;
(2) by Radix Et Caulis Acanthopanacis Senticosi, Phlomis mongolica Turcz., Chive are mixed with water, beating, filter bits, and serosity is obtained, and add brown sugar, are surpassed
High-temperature instantaneous sterilization, adds yeast bacteria agent to carry out fermentation 36 hours, and centrifugation goes supernatant, drying precipitate that mixed powder B is obtained;
The Radix Et Caulis Acanthopanacis Senticosi, Phlomis mongolica Turcz., the mass ratio that Chive is mixed with water are 1:8;The quality that the brown sugar is added accounts for grind slurries quality
3%;The ultra high temperature short time sterilization is to sterilize 6 seconds under the conditions of 145 DEG C;
(3) by strain, small molecular organic acid, fruit oligose, spirulina mix homogeneously make mixed-powder C;
(4) by mixed-powder A, mixed powder B and mixed-powder C mix homogeneously are preserved under the conditions of 4 DEG C.
Choose 100 abdominal distention patients.Symptom is:Abdominal tympanitess, epigastrium have gastric pattern and a peristalsises, bowel sound hyperaction or subtract
It is weak, tenderness and muscle tonus.Take the compositionss of the present embodiment 10 days, remission person 56;Take 20 days, remission person 77
Name, cures 60;Take 50 days, remission person 83, healing person 80.Healing person recurred 5 in 2 years.
Embodiment 4
A kind of green wood feed composition of regulating intestinal canal bacterial activity, is made up of the component of following parts by weight:Lactic acid bacteria bacterium
8 parts of powder, 8 parts of butanoic acid vibrio mycopowder, 8 parts of Ruminococcuses mycopowder, 8 parts of glycyrrhizic acid, 8 parts of shikimic acid, 1.5 parts of fruit oligose, Radix Et Caulis Acanthopanacis Senticosi 5
Part, 6 parts of spirulina, 15 parts of Fructus Hordei Germinatus (parched), 1.5 parts of Phlomis mongolica Turcz., 1.5 parts of Chive, 3 parts of Semen Castaneae, 5 parts of Semen Ginkgo;The lactic acid bacteria bacterium
Powder contains lactic acid bacteria 3X108Individual/g;Butanoic acid vibrio mycopowder contains butanoic acid vibrio 4X108Individual/g;Ruminococcuses mycopowder contains cud ball
Bacterium 4X108Individual/g.
The preparation method of the green wood feed composition of above-mentioned regulating intestinal canal bacterial activity, is carried out in accordance with the following steps:
(1) by Fructus Hordei Germinatus (parched), Semen Cucurbitae is crushed, and is crossed 200 mesh sieves, is made mixed-powder A;
(2) by Radix Et Caulis Acanthopanacis Senticosi, Phlomis mongolica Turcz., Chive are mixed with water, beating, filter bits, and serosity is obtained, and add brown sugar, are surpassed
High-temperature instantaneous sterilization, adds yeast bacteria agent to carry out fermentation 30 hours, and centrifugation goes supernatant, drying precipitate that mixed powder B is obtained;
The Radix Et Caulis Acanthopanacis Senticosi, Phlomis mongolica Turcz., the mass ratio that Chive is mixed with water are 1:5.The quality that the brown sugar is added accounts for grind slurries quality
1%;The ultra high temperature short time sterilization is to sterilize 7 seconds under the conditions of 138 DEG C;
(3) by strain, small molecular organic acid, fruit oligose, spirulina mix homogeneously make mixed-powder C;
(4) by mixed-powder A, mixed powder B and mixed-powder C mix homogeneously are preserved under the conditions of -20 DEG C.
100 diarrhea patients are chosen, disease recurs repeatedly.Symptom is:Frequently, each excrement amount is few and after having pain in the abdomen for awareness of defecation
Reinfection, has pus and blood in feces, arrange post-defecatory pain and do not alleviate.Take the compositionss of the present embodiment 15 days, remission person 61;
Take 30 days, remission person 71, cure 30;Take 60 days, remission person 83, healing person 77.Healing person 2 years
Interior recurrence 7.
Embodiment 5
A kind of green wood feed composition of regulating intestinal canal bacterial activity, is made up of the component of following parts by weight:Lactic acid bacteria bacterium
8 parts of powder, 8 parts of butanoic acid vibrio mycopowder, 8 parts of Ruminococcuses mycopowder, 15 parts of malic acid, 1.5 parts of fruit oligose, 5 parts of Radix Et Caulis Acanthopanacis Senticosi, spirulina
6 parts, 15 parts of Fructus Hordei Germinatus (parched), 1.5 parts of Phlomis mongolica Turcz., 1.5 parts of Chive, 7 parts of Semen Cucurbitae, 6 parts of Semen Cuscutae;The lactic acid bacteria mycopowder contains
Lactic acid bacteria 3X108Individual/g;Butanoic acid vibrio mycopowder contains butanoic acid vibrio 4X108Individual/g;Ruminococcuses mycopowder contains Ruminococcuses
4X108Individual/g.
The preparation method of the green wood feed composition of above-mentioned regulating intestinal canal bacterial activity, is carried out in accordance with the following steps:
(1) by Fructus Hordei Germinatus (parched), Semen Cucurbitae is crushed, and is crossed 200 mesh sieves, is made mixed-powder A;
(2) by Radix Et Caulis Acanthopanacis Senticosi, Phlomis mongolica Turcz., Semen Cuscutae, Chive are mixed with water, beating, filter bits, and serosity is obtained, and are added
Brown sugar, ultra high temperature short time sterilization add yeast bacteria agent to carry out fermentation 30 hours, and centrifugation goes supernatant, drying precipitate to be obtained mixed
Close powder B;The Radix Et Caulis Acanthopanacis Senticosi, Phlomis mongolica Turcz., the mass ratio that Chive is mixed with water are 1:5.The quality that the brown sugar is added accounts for slurry
The 1% of liquid quality;The ultra high temperature short time sterilization is to sterilize 7 seconds under the conditions of 138 DEG C;
(3) by strain, small molecular organic acid, fruit oligose, spirulina mix homogeneously make mixed-powder C;
(4) by mixed-powder A, mixed powder B and mixed-powder C mix homogeneously are preserved under the conditions of -20 DEG C.
100 diarrhea patients are chosen, disease recurs repeatedly.Symptom is:Frequently, each excrement amount is few and after having pain in the abdomen for awareness of defecation
Reinfection, has pus and blood in feces, arrange post-defecatory pain and do not alleviate.Take the compositionss of the present embodiment 15 days, remission person 75;
Take 30 days, remission person 85, cure 40;Take 60 days, remission person 99, healing person 88.Healing person 2 years
It is interior without recurrence example.
Embodiment 6
A kind of green wood feed composition of regulating intestinal canal bacterial activity, is made up of the component of following parts by weight:Hay spore
7 parts of bacillus mycopowder, 8 parts of Lactobacillus plantarum mycopowder, 5 parts of Ruminococcuses mycopowder, 10 parts of citric acid, 1 part of fruit oligose, 1 part of Radix Et Caulis Acanthopanacis Senticosi,
1 part of Herba Bidentis Bipinnatae, 3 parts of spirulina, 10 parts of Fructus Hordei Germinatus (parched), 1 part of Phlomis mongolica Turcz., 1 part of Chive, 3 parts of Semen Juglandiss;Bacillus subtilises mycopowder contains
There is bacillus subtilises 2X108Individual/g;Lactobacillus plantarum mycopowder contains Lactobacillus plantarum 7X108Individual/g;Ruminococcuses mycopowder contains
Ruminococcuses 7X108Individual/g.
The preparation method of the green wood feed composition of above-mentioned regulating intestinal canal bacterial activity, is carried out in accordance with the following steps:
(1) by Fructus Hordei Germinatus (parched), kernel is crushed, and is crossed 100 mesh sieves, is made mixed-powder A;
(2) by Radix Et Caulis Acanthopanacis Senticosi, Phlomis mongolica Turcz., Herba Bidentis Bipinnatae, Chive are mixed with water, beating, filter bits, and serosity is obtained, and are added
Brown sugar, ultra high temperature short time sterilization add yeast bacteria agent to carry out fermentation 24 hours, and centrifugation goes supernatant, drying precipitate to be obtained mixed
Close powder B;The Radix Et Caulis Acanthopanacis Senticosi, Phlomis mongolica Turcz., the mass ratio that Chive is mixed with water are 1:2;The quality that the brown sugar is added accounts for slurry
The 0.5% of liquid quality;The ultra high temperature short time sterilization is to sterilize 10 seconds under the conditions of 137 DEG C;
(3) by strain, small molecular organic acid, fruit oligose, spirulina mix homogeneously make mixed-powder C;
(4) by mixed-powder A, mixed powder B and mixed-powder C mix homogeneously are preserved under the conditions of -40 DEG C.
100 constipation patients are chosen, disease recurs repeatedly.Symptom is:Stool is reduced, and in one week, times of defecation is less than
2~3 times, or 2~3 talent stool 1 time, feces amount is few and dry and hard, with loss of appetite, abdominal distention, lower abdomen be uncomfortable, aerofluxuss are more or
There are dizziness, headache.Take the compositionss of the present embodiment 15 days, remission person 65;Take 30 days, remission person 89,
Cure 70;Take 60 days, remission person 99, healing person 98.Without recurrence example in healing person 2 years.
Embodiment 7
A kind of green wood feed composition of regulating intestinal canal bacterial activity, is made up of the component of following parts by weight:Bacillus bifiduss
10 parts of mycopowder, 10 parts of streptococcus thermophiluss mycopowder, 8 parts of Lactobacillus plantarum mycopowder, 20 parts of myristic acid, 2 parts of fruit oligose, Radix Et Caulis Acanthopanacis Senticosi 10
Part, 2 parts of Periostracum Cicadae, 10 parts of spirulina, 20 parts of Fructus Hordei Germinatus (parched), 2 parts of Phlomis mongolica Turcz., 2 parts of Chive, 5 parts of Fructus anacardii, 5 parts of Semen Nelumbinis;Bacillus bifiduss
Mycopowder contains bacillus bifiduss 3X108Individual/g;Streptococcus thermophiluss mycopowder contains streptococcus thermophiluss 5X108Individual/g.
The preparation method of the green wood feed composition of above-mentioned regulating intestinal canal bacterial activity, is carried out in accordance with the following steps:
(1) by Fructus Hordei Germinatus (parched), Periostracum Cicadae, kernel are crushed, and cross 300 mesh sieves, make mixed-powder A;
(2) by Radix Et Caulis Acanthopanacis Senticosi, Phlomis mongolica Turcz., Chive are mixed with water, beating, filter bits, and serosity is obtained, and add brown sugar, are surpassed
High-temperature instantaneous sterilization, adds yeast bacteria agent to carry out fermentation 36 hours, and centrifugation goes supernatant, drying precipitate that mixed powder B is obtained;
The Radix Et Caulis Acanthopanacis Senticosi, Phlomis mongolica Turcz., the mass ratio that Chive is mixed with water are 1:8;The quality that the brown sugar is added accounts for grind slurries quality
3%;The ultra high temperature short time sterilization is to sterilize 6 seconds under the conditions of 145 DEG C;
(3) by strain, small molecular organic acid, fruit oligose, spirulina mix homogeneously make mixed-powder C;
(4) by mixed-powder A, mixed powder B and mixed-powder C mix homogeneously are preserved under the conditions of 4 DEG C.
Choose 100 abdominal distention patients.Symptom is:Abdominal tympanitess, epigastrium have gastric pattern and a peristalsises, bowel sound hyperaction or subtract
It is weak, tenderness and muscle tonus.Take the compositionss of the present embodiment 10 days, remission person 66;Take 20 days, remission person 88
Name, cures 70;Take 50 days, remission person 99, healing person 98.Without recurrence example in healing person 2 years.
Claims (9)
1. a kind of green wood feed composition of regulating intestinal canal bacterial activity, it is characterised in that be made up of the component of following parts by weight:
Strain 15-30 parts, small molecular organic acid 10-20 parts, fruit oligose 1-2 parts, Radix Et Caulis Acanthopanacis Senticosi 1-10 parts, spirulina 3-10 parts, Fructus Hordei Germinatus (parched)
10-20 parts, Phlomis mongolica Turcz. 1-2 parts, Chive 1-2 parts, kernel 3-10 parts.
2. a kind of green wood feed composition of regulating intestinal canal bacterial activity according to claim 1, it is characterised in that the strain
Including lactic acid bacteria mycopowder, bacillus subtilises mycopowder, butanoic acid vibrio mycopowder, Bifidobacteria powder, Ruminococcuses mycopowder, plant breast
Bacillus mycopowder, one or more in streptococcus thermophiluss mycopowder.
3. a kind of green wood feed composition of regulating intestinal canal bacterial activity according to claim 2, it is characterised in that the lactic acid
Bacterium mycopowder contains lactic acid bacteria 1-8X108Individual/g;Bacillus subtilises mycopowder contains bacillus subtilises 1-8X108Individual/g;Butanoic acid arc
Bacterium mycopowder contains butanoic acid vibrio 1-8X108Individual/g;Bifidobacteria powder contains bacillus bifiduss 1-8X108Individual/g;Ruminococcuses mycopowder
Containing Ruminococcuses 1-8X108Individual/g;Lactobacillus plantarum mycopowder contains Lactobacillus plantarum 1-8X108Individual/g;Streptococcus thermophiluss mycopowder
Containing streptococcus thermophiluss 1-8X108Individual/g.
4. a kind of green wood feed composition of regulating intestinal canal bacterial activity according to claim 1, it is characterised in that described little point
Sub- organic acid is malic acid, shikimic acid, citric acid, tartaric acid, glycyrrhizic acid, one or more in myristic acid.
5. a kind of green wood feed composition of regulating intestinal canal bacterial activity according to claim 1, it is characterised in that the kernel
For Semen arachidis hypogaeae, Semen Helianthi, Semen Juglandiss, Semen Castaneae, Semen Armeniacae Amarum, Semen Cucurbitae, Semen Citrulli, Fructus anacardii, Semen Pini, Fructus Pistaciae Verae, Semen Ginkgo, the one kind in Semen Nelumbinis
Or more than one.
6. the preparation method of the green wood feed composition of regulating intestinal canal bacterial activity described in claim 1, it is characterised in that according to such as
Lower step is carried out:
(1) by Fructus Hordei Germinatus (parched), kernel is crushed, and is crossed 100-300 mesh sieves, is made mixed-powder A;
(2) by Radix Et Caulis Acanthopanacis Senticosi, Phlomis mongolica Turcz., Chive are mixed with water, beating, filter bits, and serosity is obtained, and add brown sugar, superhigh temperature
Instantaneous sterilization, adds yeast bacteria agent to carry out 24-36 hours of fermenting, and centrifugation goes supernatant, drying precipitate that mixed powder B is obtained;
(3) by strain, small molecular organic acid, fruit oligose, spirulina mix homogeneously make mixed-powder C;
(4) by mixed-powder A, mixed powder B and mixed-powder C mix homogeneously are preserved under the conditions of -40 DEG C -4 DEG C.
7. the preparation method of the green wood feed composition of regulating intestinal canal bacterial activity according to claim 6, it is characterised in that institute
Radix Et Caulis Acanthopanacis Senticosi, Phlomis mongolica Turcz. are stated, the mass ratio that Chive is mixed with water is 1:(1-10).
8. the preparation method of the green wood feed composition of regulating intestinal canal bacterial activity according to claim 6, it is characterised in that institute
The quality for stating brown sugar addition accounts for the 0.5-3% of grind slurries quality.
9. the preparation method of the green wood feed composition of regulating intestinal canal bacterial activity according to claim 6, it is characterised in that institute
It is to sterilize the 6-10 seconds under the conditions of 137-145 DEG C to state ultra high temperature short time sterilization.
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107557438A (en) * | 2017-10-12 | 2018-01-09 | 江苏省渔业技术推广中心 | A kind of method based on high-flux sequence detection freshwater shrimp enteron aisle lactic acid bacteria |
CN115678781A (en) * | 2022-12-30 | 2023-02-03 | 绿烨锦源(北京)生物科技有限公司 | Microbial inoculum for animal culture fermentation bed and preparation method thereof |
CN116114886A (en) * | 2023-03-06 | 2023-05-16 | 美益添生物医药(武汉)有限公司 | Composition for reducing pathogenic bacteria proportion in intestinal tract, and preparation method and application thereof |
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CN1208620A (en) * | 1997-08-15 | 1999-02-24 | 陈秀枢 | Composite contg. bacterium bifidum and lactobacillus |
CN101953916A (en) * | 2010-09-13 | 2011-01-26 | 杜丹 | Composition containing L. acidophilus 1.1878 and Chinese medicaments and application thereof |
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2016
- 2016-12-14 CN CN201611153825.2A patent/CN106579456A/en active Pending
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN1208620A (en) * | 1997-08-15 | 1999-02-24 | 陈秀枢 | Composite contg. bacterium bifidum and lactobacillus |
CN101953916A (en) * | 2010-09-13 | 2011-01-26 | 杜丹 | Composition containing L. acidophilus 1.1878 and Chinese medicaments and application thereof |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107557438A (en) * | 2017-10-12 | 2018-01-09 | 江苏省渔业技术推广中心 | A kind of method based on high-flux sequence detection freshwater shrimp enteron aisle lactic acid bacteria |
CN115678781A (en) * | 2022-12-30 | 2023-02-03 | 绿烨锦源(北京)生物科技有限公司 | Microbial inoculum for animal culture fermentation bed and preparation method thereof |
CN116114886A (en) * | 2023-03-06 | 2023-05-16 | 美益添生物医药(武汉)有限公司 | Composition for reducing pathogenic bacteria proportion in intestinal tract, and preparation method and application thereof |
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