CN106955335B - Traditional Chinese medicine microecological preparation for preventing calf diarrhea and preparation method thereof - Google Patents
Traditional Chinese medicine microecological preparation for preventing calf diarrhea and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicine microecological preparation for preventing calf diarrhea and a preparation method thereof, wherein the traditional Chinese medicine microecological preparation comprises compound microorganisms and a traditional Chinese medicine composition, wherein the traditional Chinese medicine composition comprises the following traditional Chinese medicine raw materials: parched fructus Hordei Germinatus, fructus crataegi, Massa Medicata Fermentata, radix astragali, endothelium corneum Gigeriae Galli, pericarpium Citri Tangerinae, cortex Phellodendri, pericarpium Granati and herba Origani; the compound microorganism comprises Lactobacillus bulgaricus, Streptococcus thermophilus, Bacillus bifidus, Bacillus subtilis, Bacillus cereus and enterococcus faecalis, and the total viable count of the compound microorganism is not less than 1 × 1010cfu/g traditional Chinese medicine microecological preparation. According to the invention, the traditional Chinese medicine microecological preparation is obtained by crushing and mixing traditional Chinese medicine raw materials and fermenting under the combined action of compound microorganisms, wherein active ingredients and active substances of the traditional Chinese medicine raw materials are extracted and utilized to the maximum extent, so that the absorption of calves is facilitated, and the new active substances generated after fermentation can further improve the disease resistance of calves and promote the growth and development of the calves.
Description
Technical Field
The invention belongs to the technical field of medicines, and particularly relates to a traditional Chinese medicine microecological preparation for preventing calf diarrhea and a preparation method thereof.
Background
Calf diarrhea (Calf diarrhea) is a common disease, can occur to calves born for 2 days to 2 months, has the mortality rate of up to 70 percent, is one of the frequently encountered diseases of the current calves, and brings great economic loss to the cattle industry. The main symptom of the disease is diarrhea, the excrement is thin soup or water sample, dehydration and acidosis can occur, and the death rate in the later period is high. The disease takes the digestive tract as a main transmission path, is rapidly transmitted and is easy to cause epidemic. Antibiotic treatment is adopted clinically at home and abroad, but the problems of drug resistance and overproof drug residue of animal products are easily caused, and the health of people and animals and public health and safety are harmed.
Some Chinese medical scholars study the problem of calf diarrhea and develop traditional Chinese medicine compositions with different formulas, thereby greatly reducing the probability of calf diarrhea and avoiding the problems of drug resistance and overproof drug residue. However, in the existing traditional Chinese medicine preparations, some traditional Chinese medicines can cause certain stimulation and damage to the gastrointestinal tract of calves, such as flora disorder, and can also affect the body condition of calves, so that the application of the traditional Chinese medicine composition is limited to a certain extent. The problem of developing an additive which can prevent diarrhea, protect calf gastrointestinal tract and promote the growth and development of calves is troublesome.
Disclosure of Invention
In order to solve the problems, the invention aims to provide a traditional Chinese medicine microecological preparation for preventing calf diarrhea and a preparation method thereof, which can promote rumen development, regulate the pH value of rumen, protect gastrointestinal tract, inhibit intestinal pathogenic microorganisms, reduce diarrhea rate, improve calf survival rate and daily gain, wean ahead of time and improve breeding benefits.
In order to achieve the purpose, the invention adopts the technical scheme that:
a traditional Chinese medicine microecological preparation for preventing calf diarrhea comprises compound microorganisms and a traditional Chinese medicine composition, wherein the traditional Chinese medicine composition comprises the following traditional Chinese medicine raw materials in parts by weight: 5-15 parts of roasted malt: 5-15 parts of hawthorn: 5-15 of medicated leaven: 5-15 parts of astragalus: 5-15 parts of endothelium corneum gigeriae galli: 5-15 parts of dried orange peel: 5-15 parts of phellodendron bark: 5-15 parts of pomegranate peel: 5-15 parts of oregano;
the compound microorganism comprises lactobacillus bulgaricus, streptococcus thermophilus, bifidobacterium, bacillus subtilis, bacillus cereus and enterococcus faecalis, and the viable bacteria number ratio is (2-1): (2-1): (2-1): (2-1): (2-1): (2-1); the total number of viable bacteria of the compound microorganism is more than or equal to 1 multiplied by 1010cfu/g traditional Chinese medicine microecological preparation.
Preferably, the traditional Chinese medicine composition comprises the following traditional Chinese medicine raw materials in parts by weight: 10, stir-frying malt: 10 of hawthorn fruit: medicated leaven 10: 10 of astragalus root: endothelium corneum gigeriae galli 10: dried orange peel 10: 10, phellodendron bark: 10, pomegranate rind: and (5) oregano 10.
A preparation method of a traditional Chinese medicine microecological preparation for preventing calf diarrhea comprises the following steps:
(1) respectively inoculating the preserved bacterium liquids of lactobacillus bulgaricus, streptococcus thermophilus, bifidobacterium, bacillus subtilis, bacillus cereus and enterococcus faecalis into respective liquid culture media in the inoculation amount of 1 per thousand, and performing shaking culture at 38 ℃ for overnight at the rotation speed of 200 rpm;
(2) weighing the traditional Chinese medicine raw materials according to the weight ratio, respectively crushing the traditional Chinese medicine raw materials, and uniformly mixing to obtain a solid base material;
(3) respectively mixing the solid base material and the culture solution of each strain in the step (1) according to the proportion of adding 20ml of culture solution to every 100g of the solid base material, and uniformly stirring;
(4) hermetically culturing the mixture of the culture solution of each strain and the solid base material in the step (3) at 38 ℃ for more than 24 hours, and measuring the number of the live bacteria of each strain culture to ensure that the number of the live bacteria of each strain culture meets the live bacteria requirement of the traditional Chinese medicine microecological preparation;
(5) drying the culture of each strain in the step (4) at 45 ℃ for 48h, crushing and subpackaging to prepare a preparation of each strain;
(6) mixing the preparations of each strain according to the viable bacteria number ratio of each strain to obtain Chinese medicinal microecological preparation, and ensuring that the total viable bacteria number is not less than 1 × 1010cfu/g traditional Chinese medicine microecological preparation.
The liquid culture medium of the lactobacillus bulgaricus comprises: 10g of peptone, 10g of beef extract, 5g of yeast extract and K2HPO42g, 5g of sodium acetate, 2g of ammonium citrate, 20g of glucose, 801ml of Tween and Mg SO4·7H2O 0.25g、Mn SO4·4H2O0.58g and distilled water 1000 ml.
The liquid culture medium of the streptococcus thermophilus comprises: 500ml of whey, 500ml of distilled water, 5g of tryptone, 1g of glucose, 2.5g of yeast extract and 20g of agar, and the pH value is 6.5.
The liquid culture medium of the bifidobacterium is as follows: 5g of tryptone, 5g of soybean peptone, 10g of yeast powder, 10g of glucose, 801ml of tween, 15-20g of agar, 0.05g of L-cysteine hydrochloride, 4ml of salt solution and 1000ml of distilled water; the salt solution is as follows: CaCl20.2g、K2HPO41.0g、KH2PO41.0g、MgSO4·7H2O 0.48g、Na2CO310g, NaCl2g, and 1000ml of distilled water.
The liquid culture medium of the bacillus subtilis and the bacillus cereus is as follows: 10g of tryptone, 3g of beef extract, 5g of sodium chloride and 1000ml of distilled water, and the pH value is 7.4.
The liquid culture medium of the enterococcus faecalis comprises: tryptone 10g, beef extract 10g, yeast powder 5g, K2HPO42g of diammonium citrate, 2g of sodium acetate, 5g of glucose, 801ml of Tween and MgSO4·7H2O 0.58g、MnSO4·4H2O0.25g and 1000ml of distilled water.
Stir-baked malt is indicated for indigestion, abdominal distension and pain, spleen deficiency with poor appetite, milk sweat accumulation, breast distension and pain, and women's lactation cessation.
Haw, fructus crataegi, sour and sweet in taste, slightly warm in nature, enters spleen, stomach and liver meridians. The functional indications are as follows: promoting digestion, removing blood stasis, expelling tapeworm. It is used for treating meat stagnation, abdominal mass, phlegm retention, abdominal distention, acid regurgitation, dysentery, intestinal wind, lumbago, infantile colic, abdominal pain, lochiorrhea, and infantile dyspepsia. Promote digestion, invigorate stomach, promote qi circulation and dissipate blood stasis. Can be used for treating dyspepsia, flatulence, dysentery, abdominal pain, blood stasis, amenorrhea, puerperal stagnation, heart and abdominal pain, hernia pain, and hyperlipidemia.
Shen Qu, sweet and pungent in flavor and warm in nature. It enters spleen and stomach meridians. The functional indications are as follows: promote digestion and harmonize stomach. It can be used for treating food stagnation, abdominal distention, anorexia, etc.
Huang Qi is sweet in flavor and slightly warm in nature. It enters lung, spleen, liver and kidney meridians. The functional indications are as follows: spontaneous perspiration due to exterior deficiency, night sweat due to yin deficiency, edema due to acute and chronic nephritis, yang qi deficiency, ulcer, lung qi deficiency, and qi deficiency.
Ji Nei jin is sweet in flavor and neutral in nature. It enters spleen, stomach, small intestine and bladder meridians. The functional indications are as follows: strengthening the stomach to promote digestion, arresting seminal emission and relieving enuresis, and treating stranguria and removing calculus.
Tangerine peel, pericarpium Citri Reticulatae is bitter and pungent in flavor and warm in nature. It enters lung and spleen meridians. The functional indications are as follows: regulate qi to invigorate spleen, dry dampness and resolve phlegm. Can be used for treating abdominal distention, anorexia, vomiting, diarrhea, cough, and excessive phlegm.
Huang Bai is bitter in taste and cold in nature. It enters kidney and bladder meridians. The functional indications are as follows: clear heat and dry dampness, purge fire and remove steam, remove toxicity and cure sore. Can be used for treating dysentery due to damp-heat pathogen, jaundice, dark urine, leukorrhagia, pudendal pruritus, pyretic stranguria, pain, tinea pedis, atrophic debility cramped, hectic fever, night sweat, nocturnal emission, pyocutaneous disease, toxic swelling, eczema, and eczema. Salted cortex phellodendri is used for nourishing yin and reducing internal heat. Can be used for treating hyperactivity of fire due to yin deficiency, night sweat and steaming bone.
Pomegranate rind, sour, astringent and warm in nature. It enters large intestine meridian. The functional indications are as follows: astringe intestines to check diarrhea, stop bleeding, expel parasites. Can be used for treating chronic diarrhea, chronic dysentery, hematochezia, rectocele, metrorrhagia, leukorrhagia, abdominal pain due to parasitic infestation.
Oregano, pungent in flavor and cool in nature. The functional indications are as follows: induce sweating and release exterior, relieve summer-heat and resolve dampness. Can be used for treating heatstroke, common cold, acute gastroenteritis, and abdominal pain.
The invention has the beneficial effects that:
1. according to the invention, by utilizing the characteristics that traditional Chinese medicines are not easy to generate drug resistance and drug residues of animal products, the traditional Chinese medicine raw materials of roasted malt, hawthorn, medicated leaven, astragalus membranaceus, endothelium corneum gigeriae galli, dried orange peel, golden cypress, pomegranate peel, oregano and the like which basically do not stimulate and damage gastrointestinal tracts of calves are selected to be scientifically compatible, and by utilizing the mutual synergistic effect of the raw materials, the intestinal pathogenic microorganisms can be effectively inhibited, the diarrhea rate of calves is reduced, the rumen development is promoted, the pH value of rumens is adjusted, the survival rate and daily gain of calves are improved.
2. The composite microorganism is another innovation point of the invention, different microorganisms are reasonably proportioned, the growth of intestinal pathogenic microorganisms can be further inhibited, the number of beneficial microorganisms in intestinal tracts is increased, the diarrhea rate of calves is reduced, and meanwhile, the addition of the composite microorganism can adjust the microenvironment in gastrointestinal tracts, adjust the pH value of rumens, promote the growth of rumens, protect the health of gastrointestinal tracts, promote the absorption of the gastrointestinal tracts to nutrient components, vitamins and mineral substances, and promote the growth and development of calves.
3. According to the invention, after the traditional Chinese medicine raw materials are crushed and mixed, the traditional Chinese medicine microecological preparation is obtained by fermentation under the combined action of lactobacillus bulgaricus, streptococcus thermophilus, bifidobacterium, bacillus subtilis, bacillus cereus and enterococcus faecalis, effective components and active substances of the traditional Chinese medicine raw materials are extracted and utilized to the greatest extent, the absorption of calves is facilitated, and the new active substances generated after fermentation can further improve the disease resistance of the calves and promote the growth and development of the calves. The product of the invention combines the active ingredients of the Chinese herbal medicines, beneficial microorganisms and other substances, and performs fermentation optimization on the basis, thereby realizing the maximization of the utilization of the Chinese herbal medicines and the microorganisms and having good social and economic benefits.
Detailed Description
The following examples further illustrate the embodiments of the present invention in detail.
The liquid culture medium used for culturing the strain can be a conventional liquid culture medium or can be prepared by self, and specifically, the liquid culture medium used in the invention comprises the following components:
the liquid culture medium of lactobacillus bulgaricus is as follows: 10g of peptone, 10g of beef extract, 5g of yeast extract and K2HPO42g, 5g of sodium acetate, 2g of ammonium citrate, 20g of glucose, 801ml of Tween and Mg SO4·7H2O 0.25g、Mn SO4·4H20.58g of O and 1000ml of distilled water.
The liquid culture medium of the streptococcus thermophilus comprises: 500ml of whey, 500ml of distilled water, 5g of tryptone, 1g of glucose, 2.5g of yeast extract and 20g of agar, and the pH value is 6.5.
The liquid culture medium of bifidobacterium is: 5g of tryptone, 5g of soybean peptone, 10g of yeast powder, 10g of glucose, 801ml of tween, 15-20g of agar, 0.05g of L-cysteine hydrochloride, 4ml of salt solution and 1000ml of distilled water; the salt solution is as follows: CaCl20.2g、K2HPO41.0g、KH2PO41.0g、MgSO4·7H2O 0.48g、Na2CO310g, NaCl2g and distilled water 1000 ml.
The liquid culture medium of the bacillus subtilis and the bacillus cereus is as follows: 10g of tryptone, 3g of beef extract, 5g of sodium chloride and 1000ml of distilled water, and the pH value is 7.4.
The liquid culture medium of enterococcus faecalis is: tryptone 10g, beef extract 10g, yeast powder 5g, K2HPO42g of diammonium citrate, 2g of sodium acetate, 5g of glucose, 801ml of Tween and MgSO4·7H2O 0.58g、MnSO4·4H20.25g of O and 1000ml of distilled water.
Example 1
A traditional Chinese medicine microecological preparation for preventing calf diarrhea comprises compound microorganisms and a traditional Chinese medicine composition, wherein the traditional Chinese medicine composition comprises the following traditional Chinese medicine raw materials in parts by weight: 10, stir-frying malt: 10 of hawthorn fruit: medicated leaven 10: 10 of astragalus root: endothelium corneum gigeriae galli 10: dried orange peel 10: 10, phellodendron bark: 10, pomegranate rind: and (5) oregano 10.
The compound microorganism comprises lactobacillus bulgaricus, streptococcus thermophilus, bifidobacterium, bacillus subtilis, bacillus cereus and enterococcus faecalis, and the number ratio of viable bacteria of the lactobacillus bulgaricus, the streptococcus thermophilus, the bifidobacterium, the bacillus subtilis, the bacillus cereus and the enterococcus faecalis is 1: 1: 1: 1: 1: 1; the total number of viable bacteria of the compound microorganism is more than or equal to 1 multiplied by 1010cfu/g traditional Chinese medicine microecological preparation.
The preparation method of the traditional Chinese medicine microecological preparation for preventing calf diarrhea comprises the following steps:
(1) respectively inoculating the preserved bacterium liquids of lactobacillus bulgaricus, streptococcus thermophilus, bifidobacterium, bacillus subtilis, bacillus cereus and enterococcus faecalis into respective liquid culture media in the inoculation amount of 1 per thousand, and performing shaking culture at 38 ℃ for overnight at the rotation speed of 200 rpm;
(2) weighing the traditional Chinese medicine raw materials according to the weight ratio, respectively crushing the traditional Chinese medicine raw materials, and uniformly mixing to obtain a solid base material;
(3) respectively mixing the solid base material and the culture solution of each strain in the step (1) according to the proportion of adding 20ml of culture solution to every 100g of the solid base material, and uniformly stirring;
(4) hermetically culturing the mixture of the culture solution of each strain and the solid base material in the step (3) at 38 ℃ for more than 24 hours, and measuring the number of the live bacteria of each strain culture to ensure that the number of the live bacteria of each strain culture meets the live bacteria requirement of the traditional Chinese medicine microecological preparation;
(5) drying the culture of each strain in the step (4) at 45 ℃ for 48h, crushing and subpackaging to prepare a preparation of each strain;
(6) mixing the preparations of each strain according to the viable bacteria number ratio of each strain to obtain Chinese medicinal microecological preparation, and ensuring that the total viable bacteria number is not less than 1 × 1010cfu/g traditional Chinese medicine microecological preparation.
Example 2
A traditional Chinese medicine microecological preparation for preventing calf diarrhea comprises compound microorganisms and a traditional Chinese medicine composition, wherein the traditional Chinese medicine composition comprises the following traditional Chinese medicine raw materials in parts by weight: 15, stir-frying malt: 5, hawthorn fruit: medicated leaven 15: 5, astragalus root: endothelium corneum gigeriae galli 5: and (3) dried orange peel 5: 15, phellodendron bark: pomegranate rind 5: and 5, oregano.
The compound microorganism comprises lactobacillus bulgaricus, streptococcus thermophilus, bifidobacterium, bacillus subtilis, bacillus cereus and enterococcus faecalis, and the viable bacteria number ratio of the lactobacillus bulgaricus, the streptococcus thermophilus, the bifidobacterium, the bacillus subtilis, the bacillus cereus and the enterococcus faecalis is 2: 2: 1: 1: 1: 2; the total number of viable bacteria of the compound microorganism is more than or equal to 1 multiplied by 1010cfu/g traditional Chinese medicine microecological preparation.
The preparation method of the traditional Chinese medicine microecological preparation is the same as that of the example 1.
Example 3
A traditional Chinese medicine microecological preparation for preventing calf diarrhea comprises compound microorganisms and a traditional Chinese medicine composition, wherein the traditional Chinese medicine composition comprises the following traditional Chinese medicine raw materials in parts by weight: 5, roasted malt: 15 parts of hawthorn fruit: medicated leaven 5: 15 of astragalus root: endothelium corneum gigeriae galli 15: 15, dried orange peel: and (5) golden cypress: pomegranate rind 15: and (5) oregano 15.
The compound microorganism comprises lactobacillus bulgaricus, streptococcus thermophilus, bifidobacterium, bacillus subtilis, bacillus cereus and enterococcus faecalis, and the number ratio of viable bacteria of the lactobacillus bulgaricus, the streptococcus thermophilus, the bifidobacterium, the bacillus subtilis, the bacillus cereus and the enterococcus faecalis is 1: 1: 1: 2: 2: 2; the total number of viable bacteria of the compound microorganism is more than or equal to 1 multiplied by 1010cfu/g traditional Chinese medicine microecological preparation.
The preparation method of the traditional Chinese medicine microecological preparation is the same as that of the example 1.
Application test
1. Test materials
Animal experiments are carried out in a certain pasture, 180 calves with similar weights and 10 days of birth are selected and randomly divided into 6 groups, namely an experimental group 1-3, a control group 1-2 and a blank control group, each group has 3 repetitions, and each repetition has 10 calves. The test groups 1 to 3 were prepared by adding 2% of the microbial ecological agent and the supplementary feed granules of the traditional Chinese medicine of the examples 1 to 3 to milk, respectively, the control group 1 was prepared by adding the compound microorganism and the supplementary feed granules of the example 1 to milk, the control group 2 was prepared by adding 2% of the traditional Chinese medicine composition and the supplementary feed granules of the example 1 to milk, and the blank control group was prepared by only feeding milk and the supplementary feed granules. The addition amount of the supplementary feeding granule is conventional feeding amount, so that calves can feed freely, and less feeding is frequently added.
2. Feeding management
The test period is 50 days, during which the calf can freely take food and drink water. Daily management such as sanitary cleaning, immunity prevention, colony house disinfection and the like is carried out in the experimental process according to the requirements of a pasture.
3. Measurement items and methods
(1) Daily gain. The fasting body weight of the test calf is weighed head by head before feeding in the morning on the 1 st day as the initial body weight, the fasting body weight of the test calf is taken as the final body weight when the test is finished, and the average daily gain is recorded to calculate the daily gain (final weight-initial weight)/days.
(2) The incidence of diarrhea. And calculating the diarrhea incidence of the test calves of different groups in the test period.
4. Results and analysis
Table 1: influence of different additives on daily weight gain and diarrhea rate of calf
As can be seen from table 1: the test group and the control group have the function of preventing calf diarrhea, but the effect of the test group is obviously better than that of the control group, and the additive of the test group promotes the absorption of the nutrient substances of animal intestinal tracts to the maximum extent, so that the daily gain of calves is obviously improved.
The foregoing description is only a preferred embodiment of the present invention, and various modifications and changes will occur to those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (7)
1. The traditional Chinese medicine microecological preparation for preventing calf diarrhea is characterized by being prepared from compound microorganisms and a traditional Chinese medicine composition, wherein the traditional Chinese medicine composition is prepared from the following traditional Chinese medicine raw materials in parts by weight: 10 parts of roasted malt, 10 parts of hawthorn, 10 parts of medicated leaven, 10 parts of astragalus root, 10 parts of chicken's gizzard-membrane, 10 parts of dried orange peel, 10 parts of golden cypress, 10 parts of pomegranate rind and 10 parts of origanum vulgaris;
the compound microorganism is BulgariaThe bacillus subtilis comprises lactobacillus, streptococcus thermophilus, bifidobacterium, bacillus subtilis, bacillus cereus and enterococcus faecalis, wherein the ratio of the number of viable bacteria is (2-1) to (2-1): (2-1): (2-1); the total number of viable bacteria of the compound microorganism is more than or equal to 1 multiplied by 1010cfu/g traditional Chinese medicine microecological preparation.
2. A method for preparing the traditional Chinese medicine microecological preparation for preventing calf diarrhea according to claim 1, comprising the steps of:
(1) respectively inoculating the preserved bacterium liquids of lactobacillus bulgaricus, streptococcus thermophilus, bifidobacterium, bacillus subtilis, bacillus cereus and enterococcus faecalis into respective liquid culture media in the inoculation amount of 1 per thousand, and performing shaking culture at 38 ℃ for overnight at the rotation speed of 200 rpm;
(2) weighing the traditional Chinese medicine raw materials according to the weight ratio, respectively crushing the traditional Chinese medicine raw materials, and uniformly mixing to obtain a solid base material;
(3) respectively mixing the solid base material and the culture solution of each strain in the step (1) according to the proportion of adding 20ml of culture solution to every 100g of the solid base material, and uniformly stirring;
(4) hermetically culturing the mixture of the culture solution of each strain and the solid base material in the step (3) at 38 ℃ for more than 24 hours, and measuring the number of the live bacteria of each strain culture to ensure that the number of the live bacteria of each strain culture meets the live bacteria requirement of the traditional Chinese medicine microecological preparation;
(5) drying the culture of each strain in the step (4) at 45 ℃ for 48h, crushing and subpackaging to prepare a preparation of each strain;
(6) mixing the preparations of each strain according to the viable bacteria number ratio of each strain to obtain Chinese medicinal microecological preparation, and ensuring that the total viable bacteria number is not less than 1 × 1010cfu/g traditional Chinese medicine microecological preparation.
3. The method for preparing a microecological preparation of a traditional Chinese medicine for preventing calf diarrhea according to claim 2, wherein the liquid culture medium of Lactobacillus bulgaricus is: 10g of peptone, 10g of beef extract, 5g of yeast extract and K2HPO42g, 5g sodium acetate, 2g ammonium citrate, 20g glucose, 801ml Tween, MgSO4·7H2O 0.25g、MnSO4·4H20.58g of O and 1000ml of distilled water.
4. The method for preparing a microecological preparation of a traditional Chinese medicine for preventing calf diarrhea according to claim 2, wherein the liquid culture medium of Streptococcus thermophilus is: 500ml of whey, 500ml of distilled water, 5g of tryptone, 1g of glucose, 2.5g of yeast extract and 20g of agar, and the pH value is 6.5.
5. The method for preparing a microecological preparation of a Chinese medicinal composition for preventing calf diarrhea according to claim 2, wherein the liquid culture medium of Bifidobacterium is: 5g of tryptone, 5g of soybean peptone, 10g of yeast powder, 10g of glucose, 801ml of tween, 15-20g of agar, 0.05g of L-cysteine hydrochloride, 4ml of salt solution and 1000ml of distilled water; the salt solution is as follows: CaCl20.2g、K2HPO41.0g、KH2PO41.0g、MgSO4·7H2O 0.48g、Na2CO310g, NaCl2g and distilled water 1000 ml.
6. The preparation method of the traditional Chinese medicine microecological preparation for preventing calf diarrhea according to claim 2, wherein the liquid culture medium of the bacillus subtilis and the bacillus cereus is: 10g of tryptone, 3g of beef extract, 5g of sodium chloride and 1000ml of distilled water, and the pH value is 7.4.
7. The method for preparing a microecological preparation of a traditional Chinese medicine for preventing calf diarrhea according to claim 2, wherein the liquid medium of enterococcus faecalis is comprises: tryptone 10g, beef extract 10g, yeast powder 5g, K2HPO42g of diammonium citrate, 2g of sodium acetate, 5g of glucose, 801ml of Tween and MgSO4·7H2O 0.58g、MnSO4·4H20.25g of O and 1000ml of distilled water.
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| CN111557382A (en) * | 2020-05-22 | 2020-08-21 | 开封嘉骏生物科技有限公司 | Functional additive for preventing milk accumulation and diarrhea of calves and lambs and preparation method thereof |
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| CN114949127B (en) * | 2020-12-31 | 2023-04-28 | 河南牧业经济学院 | Traditional Chinese medicine microecological preparation for treating calf diarrhea |
| CN114159502B (en) * | 2021-11-01 | 2022-11-08 | 齐鲁工业大学 | Anti-diarrhea traditional Chinese medicine composition fermented by bacillus cereus, preparation method and application |
| CN115216429B (en) * | 2022-07-06 | 2024-06-07 | 安徽天安生物科技股份有限公司 | Composite microbial agent prepared based on hawthorn water extract and preparation method and application thereof |
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