A kind of probiotics fermention functional food for improving male's inferior health and preparation method thereof
Technical field
The invention belongs to microbial fermentations to produce field of functional food, relates to the use of probiotics fermention turmeric, sealwort, black
Pepper, rape pollen, black soya bean, raspberry, matrimony vine produce the preparation method of functional food and functional food, the work(
Energy property food is mainly used for improving male's sub-health state, including improving male prostate problem and sexual function, the functionality
Food has effects that dispelling effects of alcohol and nourishing liver simultaneously.
Background technology
As society develops rapidly, world today's competition is increasing, and thing followed life increasingly increases with operating pressure
It is long, many people, especially male is caused to be in sub-health state, and then many males is caused different degrees of prostate occur and are asked
Topic and sex dysfunction.Prostate is the distinctive sexual glands of male, and prostate problems can not only cause some local symptoms,
Such as frequent micturition, urgent urination, urethra cusalgia are more likely to result in the change of other functions of body, such as, influence sexual function, fertility, interior
Dyssecretosis causes infection, insanity, forms hyperplasia, causes canceration etc..Male sexual disfunction refer to male's sexual and
Sexual satisfaction is incompetent, according to the literature, Chinese male sex dysfunction illness rate, between 40-49 Sui, illness rate 32%, 50-59
Illness rate is up to 74% between illness rate is 36%, 60-69 Sui between year.In addition, China consumes big country, mistake as a drinks
The phenomenon that amount is drunk is not uncommon for, and ethyl alcohol has direct toxic action to the histoorgan of human body in wine, the device most sensitive to ethyl alcohol
Official is liver.Continuous excessive consumption of alcohol energy loss impariment of the liver cell, disturbs the eubolism of liver, and then alcoholic hepatitis and liver can be caused hard
Change.
It is short on the drug or effective time that improve male prostate problem and sex dysfunction on the market and aggravate liver kidney
Burden or side effect are big, may damage body, aggravate the state of an illness, there are very big hidden danger.And current relieving alcoholism and protecting liver product both at home and abroad is such as
Diuresis class chemical synthetic drug, liver-protecting and stomach-nourishing class Chinese medicine, albumen peptide medicament, more side effects are apparent or slowly effect, therapeutic effect
The bad or mechanism of action is indefinite, and there are security risks.Turmeric, black pepper, sealwort, rape pollen, black soya bean, raspberry, matrimony vine
Plants is waited just to play the role of supplementing the kidney to control the nocturnal, boosting qi and nourishing yin in traditional Chinese medicine system, qi-restoratives strengthening the essence, nourish the liver to improve visual acuity, in recent years, section
Learn research and also indicate that more than plant is improving male's inferior health, anti-prostatic hyperplasia and tumor of prostate, improve male's sexual,
Sexual deterioration, dispelling effects of alcohol and nourishing liver etc. is delayed to have good effect, currently also has and is much changed using natural plant composition
Kind male's sub-health state, improves male's sexual and prostate problems, the patent of dispelling effects of alcohol and nourishing liver:
Publication number:The invention of CN105456522A《A kind of composition for improving male's sexual and application》Disclose one kind
The composition and application, its composition for improving male's sexual are to form Fructus Rubi extract 15-30 by following components by weight
Part, 15-30 parts of oyster water extract, 10-20 parts of Fructus lycii P.E, 10-20 parts of grapefruit powder, 10-20 parts of seabuckthorn fruit powder, ginseng carry
Take 1-10 parts of object, 1-10 parts of Maca extract.
Publication number:The invention of CN106390004A《A kind of the treatment of sexual dysfunction, the composition of prostatic disorders and its system
Preparation Method》A kind of the treatment of sexual dysfunction, the composition and preparation method thereof of prostatic disorders are disclosed, the composition includes
The following mass fraction formula components fruit of Chinese wolfberry, dogwood fruit, cultivated land, Radix Angelicae Sinensis, Cortex Eucommiae, rhizome of chuanxiong, Radix Codonopsis, sealwort, Morinda officinalis, thizoma curculiginis,
The fruit of Cherokee rose, psoralea corylifolia, Herba Epimedii, Schisandra chinensis, Semen Cuscutae, Chinese cassia tree, Gorgon fruit, rhizoma cibotii, frutus cnidii, fructus alpiniae oxyphyllae, Herba Cistanches, Radix Glycyrrhizae,
It is Radix Astragali, the fruit of glossy privet, mulberries, mulberry Piao sheaths, cardamom, flower bud perfume, dried orange peel, sea Piao sheath, actinolite, felwort, rhizoma alismatis, Ramulus Taxilli, floating small
Wheat, hedgehog skin, Du's root bark of tree peony, acorus graminens soland and asarum.To there is the drug matching of nourishing kidney yin, warming and invigorating kidney Yang, pass through conjunction
Reason adjusts the proportioning of each formula, there is broad-spectrum action, without side-effects, curative effect to show the treatment of sexual dysfunction and prostatic disorders
Work, healing cycle are short.
Publication number:The invention of CN102233086A《A kind of health care Chinese medicine for improving inferior health and improving male's energy》It is open
A kind of improvement inferior health improves male's essence, gas, the health care Chinese medicine of god.It is with sea-buckthorn, flower of Panax ginseng, Cortex Eucommiae, Schisandra chinensis, fragrant-flowered garlic
Vegetable seeds, the fruit of Chinese wolfberry, Herba Epimedii, Semen Cuscutae, the fruit of Cherokee rose, Fructus Corni, wilsonii, Herba Cistanches, Bostrychia montagnei, dried orange peel, oolong tea, sweetleaf
Chrysanthemum forms.The effect of with supplementing male sex hormone, delaying male sex hormone early ageing.
The invention of open (bulletin) number CN102579703A《A kind of improvement male's sexual product and preparation method thereof》It is public
A kind of improvement male's sexual product and preparation method thereof is opened, raw material includes following components and parts by weight content:Cordyceps sinensis is extracted
Object 10-20, tribulus fruit extract 10-20, grape extract 20-30, Pulp Citrulli extract 5-15, sea cucumber extract 8-15, rhodiola root
Extract 5-15, HERBA EPIMEDII extract 10-20.Compared with prior art, the present invention is combined with traditional Chinese and western medicine theory, under improvement
It is studied based on hypothalamic-pituitary-gonad index, strengthens excited adjusting maincenter function, adjust endocrine, take a tonic or nourishing food to build up one's health vigour,
Physiological function is improved, immunological regulation, antifatigue, anti-aging extend the time, enhance gonad function, resist norepinephrine, expand
Cavernous body, by more than too many levels work, come reach it is more effective, more comprehensively, more scientific improvement male's sexual
Purpose.
The invention of open (bulletin) number CN101628073A《It is a kind of for improve male's sexual, antifatigue preparation and
Its preparation method》It discloses a kind of for improving male's sexual, antifatigue preparation and preparation method thereof.Wherein preparation is by black
Beans, semen allii tuberosi, Chinese yam, Poria cocos, matrimony vine, Semen Cuscutae, arginine and zinc gluconate are with 140-210: 120-180: 115-175:
35-55: 30-60: 25-40: 20-35: 0.5-1.5 weight ratio is made.Provided by the present invention for improving male's sexual, resisting
Preparation of fatigue and preparation method thereof is to extract the active ingredient in medicinal plants using the method for boiling extraction, and keeps it
Then activity adds in basic material as replenishing essence production of sperm of arginine, zinc gluconate, so can not only make in drug effectively into
The dissolution rate divided greatly improves, and contributes to the absorption of drug, so as to improve its bioavilability.
The invention of publication number CN104248736A《A kind of Chinese drugs agentia of dispelling effects of alcohol and nourishing liver》Disclose a kind of dispelling effects of alcohol and nourishing liver
Chinese drugs agentia.By following components by weight:15-25 parts of rhizoma imperatae, 15-25 parts of galangal, 20-30 parts of mulberry,
15-25 parts of cloves, 10-20 parts of Radix Angelicae Sinensis, 10-20 parts of grass-leaved sweetflag, 5-15 parts of plantain seed, 5-15 parts of rhizome of chuanxiong, Chu is 5-15 parts sub- in fact, will
Said components add in 300-500 parts of water in, boil 20 minutes, decoction.Medicament provided by the present invention, scientific formula are used
Amount is reasonable, not only can effectively relieve the effect of alcohol, more can blood-nourishing tonifying liver, protect liver from alcohol damaged, conserve liver.
Above-mentioned patent is disclosed to improve male's sexual and prostate problems or dispelling effects of alcohol and nourishing liver using natural plant composition
Food and drug, preparation method is simply mixed in a certain ratio after simple processing is extracted, and utilizes probiotics to natural
Plant carries out fermentation and prepares to improve male's sub-health state and take into account the functional food of dispelling effects of alcohol and nourishing liver to have not been reported.
The content of the invention
It is contemplated that in view of the shortcomings of the prior art, providing a kind of improvement male's sub-health state takes into account dispelling effects of alcohol and nourishing liver effect
Functional beverage and food, and the preparation method of the drink and food is provided, especially for having prostate problems and male
Sexual function problem and the crowd often to drink beyond one's capacity, and provide Research Thinking for the production of Related product.
The present invention passes through the productions such as probiotics fermention turmeric and black pepper, sealwort, rape pollen, black soya bean, raspberry, matrimony vine
Functional drink and food can not only retain its active ingredient, also with health role to body.Turmeric belongs to perennial
Herbaceous plant wherein the curcumin contained is a kind of phenol sulfonate, has anti-inflammatory, anti-oxidant, chemoprevention of cancer, protection liver
The effects that.The study found that curcumin can inhibit the growth of prostate gland cancer cell and induce the apoptosis of prostate gland cancer cell, to urgency
Property alcoholic liver injury has protective effect.There is good development prospect in terms of the treatment of prostate cancer and anti-liver injury.But
Traditional method for extracting curcumin difficulty is big, content is few, and is difficult to the utilization that is absorbed by the body.The present invention is by turmeric and black pepper, Huang
The cooperations such as essence, and probiotics fermention method is used, the content of curcumin and its derivative is improved, and curcumin can be promoted in human body
Interior absorption, this functional food of gain is improve in terms of male's sub-health state and dispelling effects of alcohol and nourishing liver the effect of.
It is an object of the invention to mix bacterium to turmeric, sealwort using probiotics, further include if necessary rape pollen, black soya bean,
Raspberry, matrimony vine, black pepper are fermented, and obtain a kind of probiotics functional food.
To reach above-mentioned purpose, technical scheme is as follows:
A kind of probiotics fermention functional food for improving male's inferior health, is by turmeric, sealwort, further includes if necessary auxiliary
Material, ferments in proportion;Using lactobacillus plantarum, animal bifidobacteria, Lactobacillus paracasei, bifidobacterium adolescentis, condensation gemma
Bacillus and a variety of bacterial strains of bacillus subtilis are prepared after carrying out mixed bacterium solution state or solid state fermentation;
The auxiliary material is preferably at least one of black pepper, rape pollen, black soya bean, raspberry, matrimony vine;
The present invention provides a kind of prebiotic strains liquid fermentation drinks for improving male's inferior health, fermented and cultured based raw material group
Into as follows:The curcuma powder of 2-8% (mass volume ratio), the sealwort powder of 2-8% are added in water, while can also be added in not more than
It is 5% black pepper, not more than 3% rape pollen, not more than 3% black bean powder, not more than 3% raspberry powder, few
One or more in 3% wolfberry fruit powder, stir evenly;
Preferably, the prebiotic strains liquid fermentation drinks for improving male's inferior health, fermented and cultured based raw material composition
Further include the raspberry of the black pepper of 1-5% and/or the black bean powder and/or 1-3% of the rape pollen of 1-3% and/or 1-3%
The wolfberry fruit powder of powder and/or 1-3%.
The present invention provides a kind of preparation method for the prebiotic strains liquid fermentation drinks for improving male's inferior health, specific to walk
It is rapid as follows:
(1) by turmeric, black pepper, sealwort, black soya bean, raspberry, matrimony vine cleaning be placed on 60 DEG C of oven for drying 3-4 it is small when,
Then crush, be milled 100 mesh sieves respectively, obtains curcuma powder, black pepper, sealwort powder, black bean powder, raspberry powder, wolfberry fruit powder.
(2) activation medium is prepared:12g black bean powders are added in per 100ml water, obtain activation medium;To activation medium
It sterilizes, condition is 121 DEG C, 20min;
(3) fermentation medium is prepared:The fermentation medium is:Curcuma powder, sealwort powder are added in water, it can also be simultaneously
Add the one or more in black pepper, rape pollen, black bean powder, raspberry powder, wolfberry fruit powder;
It is described preparation fermentation medium be specially:2-8g curcuma powders, 2-8g sealwort powder are added in per 100ml water, in addition may be used
The black pepper and/or 1-3g rape pollens and/or the raspberry powder and/or 1- of 1-3g black bean powders and/or 1-3g of addition 1-5g
The wolfberry fruit powder of 3g obtains liquid state fermentation culture medium;
(4) actication of culture:Lactobacillus plantarum, animal bifidobacteria, Lactobacillus paracasei, bifidobacterium adolescentis and condensation bud
Spore bacillus, bacillus subtilis glycerol tube respectively by 1% (volume ratio of v/v activation mediums) be inoculated in activation medium into
Row activation, pH is naturally, controlled at 37 DEG C;After activating for 24 hours respectively, by 10% (v/v) connect bacterium amount again transfer activation training
It supports base and carries out re-activation, reactivation obtains secondary seed solution afterwards for 24 hours;
(5) probiotics fermention:It is 10 by the inoculum concentration of animal bifidobacteria6-107CFU/g, the inoculum concentration of lactobacillus plantarum
For 106-107CFU/g, the inoculum concentration of Lactobacillus paracasei is 105-106CFU/g, the inoculum concentration of bifidobacterium adolescentis is 106-
107CFU/g, the inoculum concentration of bacillus coagulans is 105-106CFU/g, the inoculum concentration of bacillus subtilis is 105-106CFU/g
It is inoculated in fermentation medium, mixed-strains liquid fermentation 24-48h, temperature is 37 DEG C;
(6) after fermentation, zymotic fluid improves male to obtain the final product through emulsifying plus after stabilizer, tune acid, blending, homogeneous, sterilization
The prebiotic strains liquid fermentation beverage of inferior health;
The turmeric, black pepper, sealwort, rape pollen, black soya bean, raspberry, matrimony vine are commercially available;
Preferably, the composition of liquid state fermentation culture medium is:8g curcuma powders, 8g sealwort powder, the black Hus of 4g are added in per 100ml water
Green pepper powder, 3g rape pollens, 2g black bean powders, 1g raspberry powders, 1g wolfberry fruit powders;
Preferably, the mixed-strains liquid fermentation time is for 24 hours;
Preferably, the lactobacillus plantarum be lactobacillus plantarum (Lactobacillus plantarum) TK9, the bacterial strain
China Committee for Culture Collection of Microorganisms's common micro-organisms center, address are preserved on December 17th, 2015:Beijing
No. 3 Institute of Microorganism, Academia Sinica of institute of city Chaoyang District North Star West Road 1, postcode 100101, deposit number CGMCC
No.11891;This lactobacillus plantarum is with preferable anti-hydrochloric acid in gastric juice, the ability of anti-cholate, the 2h survival rates 88.03% in pH 3,
0.3% cholate 4h survival rates 81.06%, and there is extensive antifungal activity, additional preservative need not be added, it can also
Extend the drink and food preservation time.It is wider than by Wang Hai and is collected in natural fermented acid in Chinese Residents In Bao Tou City man in July, 2009
Congee;
Preferably, the animal bifidobacteria is animal bifidobacteria (Bifidobacterium animalis) 937, should
Bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, address on December 17th, 2015:
Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica, postcode 100101, deposit number CGMCC
No.11892;It is wider than by Wang Hai and is collected in Tianjin City, China in May, 2007;
Preferably, the Lactobacillus paracasei is Lactobacillus paracasei (Lactobacillus paracasei) TK1501,
The bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, ground on October 21st, 2016
Location:Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica, postcode 100101, deposit number are
CGMCC No.13130;In August, 2015 is wider than by Wang Hai and is collected in natural fermented acid congee in Chinese Residents In Bao Tou City man;
Preferably, the bifidobacterium adolescentis is bifidobacterium adolescentis (Bifidobacterium adolescentis)
TK99, the bacterial strain are preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on July 14th, 2016,
Address:Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica, postcode 100101, deposit number
For CGMCC No.12784;It is wider than by Wang Hai and is collected in Tianjin City, China in January, 2015;
Preferably, the bacillus coagulans be bacillus coagulans (Bacillus coagulans) TQ33, the bacterial strain
China Committee for Culture Collection of Microorganisms's common micro-organisms center was preserved in 9th in September in 2011, address is:Beijing
The institute 3 of city Chaoyang District North Star West Road 1, Institute of Microorganism, Academia Sinica, postcode 100101, deposit number are:CGMCC
No.5233;It is wider than by Wang Hai and is collected in the skimmed milk power of Tianjin City, China in May, 2002;
Preferably, the bacillus subtilis is bacillus subtilis (Bacillus subtilis) TK-1, and the bacterial strain is
China Committee for Culture Collection of Microorganisms's common micro-organisms center is preserved on April 2nd, 2011, address is:Beijing
The institute 3 of Chaoyang District North Star West Road 1, Institute of Microorganism, Academia Sinica, postcode 100101, deposit number CGMCC
No.4731;It is wider than by Wang Hai and is collected in inner mongolia Huhehaote in October, 2008.
Present invention simultaneously provides a kind of preparation method for the probiotics solid state fermentation food for improving male's inferior health, including such as
Lower step:Using turmeric, black pepper, sealwort, rape pollen, black soya bean, raspberry, matrimony vine as fermentation substrate, using plant breast bar
Bacterium, animal bifidobacteria, Lactobacillus paracasei, bifidobacterium adolescentis, bacillus coagulans and bacillus subtilis mix through solid-state
It is prepared after bacterium fermentation;
A kind of preparation method for the probiotics solid state fermentation functional food for improving male's inferior health, is as follows:
(1) curcuma powder, sealwort powder and black pepper, rape pollen, black bean powder, raspberry powder, wolfberry fruit powder are chosen wherein
One or several kinds are mixed by certain weight ratio, obtain the mixture of turmeric, sealwort and auxiliary material:Water=1:Solid-state is made in 0.8-2
Fermentation medium;The mixture of the turmeric, sealwort and auxiliary material:The ratio of water is weight ratio;
(2) sterilize to solid-state fermentation culture medium, condition is 121 DEG C, 20min;
(3) actication of culture:Mode is the same as above-mentioned liquid state fermentation;
(4) secondary seed solution is accessed in solid-state fermentation culture medium so that in every gram of solid medium, animal bifidobacteria
Inoculum concentration be 106-107CFU/g, the inoculum concentration of lactobacillus plantarum is 106-107CFU/g, the inoculum concentration of Lactobacillus paracasei are
105-106CFU/g, the inoculum concentration of bifidobacterium adolescentis is 106-107CFU/g, the inoculum concentration of bacillus coagulans is 105-
106CFU/g, the inoculum concentration of bacillus subtilis is 105-106CFU/g;
(5) mixed culture solid state fermentation 24-48h, 37 DEG C;
After fermentation, after being positioned over -80 DEG C of pre-freeze 12h, it is put into vacuum freeze drier and freezes 12h, turmeric is made
Probiotics solid state fermentation food;
The turmeric, black pepper, sealwort, rape pollen, black soya bean, raspberry, matrimony vine are commercially available;
Preferably, the mixture of the turmeric, sealwort and auxiliary material:Water=1:1.2;
Preferably, in the solid state fermentation, vacuum freeze drying condition carries out vacuum freeze drying after being -80 DEG C of pre-freeze 6h
12h, until water content is not more than 5%;
Preferably, the lactobacillus plantarum is lactobacillus plantarum (Lactobacillus plantarum) TK9, and preservation is compiled
Number be CGMCC No.11891;
Preferably, the animal bifidobacteria is animal bifidobacteria (Bifidobacterium animalis) 937, is protected
It is CGMCC No.11892 to hide number;
Preferably, the Lactobacillus paracasei is Lactobacillus paracasei (Lactobacillus paracasei) TK1501,
Deposit number is CGMCC NO.13130;
Preferably, the bifidobacterium adolescentis is bifidobacterium adolescentis (Bifidobacteriumadolescentis)
TK99, deposit number are CGMCC No.12784;
Preferably, the bacillus coagulans is bacillus coagulans (Bacillus coagulans) TQ33, and preservation is compiled
Number it is:CGMCC No.5233;
Preferably, the bacillus subtilis be bacillus subtilis (Bacillus subtilis) TK-1, deposit number
For CGMCC No.4731.
Advantageous effect
Turmeric belongs to herbaceous plant for many years, wherein the curcumin contained is a kind of phenol sulfonate, curcumin have it is anti-oxidant,
Many-sided pharmacology such as free radical and protection cardiovascular system and protection liver is removed in anti-inflammatory, antitumor, antimicrobial, reducing blood lipid
Effect.The study found that curcumin can inhibit the growth of prostate gland cancer cell and induce the apoptosis of prostate gland cancer cell, to acute
Alcoholic liver injury has protective effect.There is good development prospect in terms of the treatment of prostate cancer and anti-liver injury.
The entire plant of black pepper all has medicinal and edible value, is usually used in treating constipation, chronic malaria, gonorrhoea, abdomen
The diseases such as rush down, black pepper also has anti-inflammatory, anticancer, alleviates depressed, hypoglycemic, reducing blood lipid, weight-reducing, treats the work(such as hepatic injury in addition
Can, can also stimulating intestine and stomach, promote intestines peristalsis.Pipering is one of major physiological active ingredient in black pepper, belongs to cassia bark
Amide alkaloid has a variety of pharmacological activities such as anticonvulsion, anticancer, anti-oxidant, anti-inflammatory, promotion drug absorption.Pipering
Effect as bioavilability reinforcing agent is increasingly taken seriously, can improve medicine from multipaths such as absorption, metabolism
The bioavilability of object, therefore, addition black pepper can improve absorbing for curcumin in fermentation.
Sealwort is mild-natured, sweet in flavor, nontoxic, returns spleen, lung, kidney channel, has tonifying middle-Jiao and Qi, yin-nourishing invigorating the spleen, moistening lung kidney-nourishing, strengthening the bones and muscles
Etc. functions, for deficiency of spleen-QI and stomach-QI, fatigue and asthenia, deficiency of stomach-Yin, dry deficiency of food, deficiency syndrome of the lung cough caused by dryness, overstrain cough hemoptysis, asthenia of essence and blood, waist
The diseases such as knees soreness, poliosis, Heat Diabetes.Modern pharmacology research proves, sealwort have enhancing immune function, Adjust-blood lipid,
The multiple pharmacological effects such as hypoglycemic, anti-aging, the clinical treatment available for diabetes, coronary heart disease, hyperlipidemia etc..
Rape pollen is full of nutrition, comprehensive, has plurality of health care functions, improving immunocompetence, antifatigue, reinforcement
Power, brain tonic and intelligence development, anti-hypoxemia, adjusting endocrine, pre- anti-aging, skin maintenance etc., meanwhile, to cardiovascular and cerebrovascular disease, prostate
Disease, liver disease, habitual constipation, anaemia, diabetes etc. have certain treatment and auxiliary treatment functio.Domestic and international clinical practice is equal
Prove that pollen has good treatment chronic non-bacterial prostatitis, prostate pain, benign prostatic hyperplasis and prostate
The effect of cancer.The effective component of pollen treatment prostatic disorders is mainly aliphatic acid, polyphenol, flavonoids, steroidal in its component
And nitrogenous compound.
Black soya bean is sweet in flavor, mild-natured, and returns spleen, kidney channel have effects that benefiting shrewd head, nourishing the blood to expel wind, Li Shui, removing toxic substances.Modern times face
Bed and pharmacological research show:Black soya bean has anti-oxidant, adjusting hormonal readiness, prevention of arterial vascular sclerosis, cosmetic hair-care, macrobiosis benefit
The effects that intelligence, cancer-resisting.
Raspberry, sweet in flavor, slightly astringent taste, the flavones contained, organic acid, carbohydrate, vitamin, ellagic acid, cupreol and
Trace elements etc. have the effect of anti-aging, adjust reproductive system, promote cellular immunity function, lose weight, have supporing yang contracting urine, kidney tonifying
Controlling nocturnal emission with astringent drugs function, for treating the illnesss such as impotence, emission due to the kidney deficiency, frequent micturition and the enuresis.
Raspberry category rose family rubus Berry plant, dry fruit is medicine-food two-purpose fruit, rich in vitamin E, super oxygen
The anti-agings substance such as object mutase, aminobutyric acid;Its nature and flavor is sweet, sour, and tepor returns liver and kidney channel, invigorates the kidney and stops nocturnal emission, supporing yang contracting urine
Function can treat the illnesss such as emission due to the kidney deficiency, impotence, the enuresis and frequent micturition.In addition, raspberry also containing abundant cellulose and K,
The various trace elements such as Zn, Fe, Cu, Mn, long-term consumption can effectively act as cardioprotection, prevent angiocardiopathy, anticancer with
Antiobesity action has higher medicinal health Development volue.
The fruit of Chinese wolfberry is sweet in flavor, mild-natured, returns liver and kidney channel, there is tonifying liver, kidney-nourishing, the function of improving eyesight, available for treatment consumption consumptive loss,
The illnesss such as soreness of waist and knee joint.The fruit of Chinese wolfberry is the Chinese medicine material of nutritional supplement and food, wherein the polysaccharides contained is matrimony vine
The critical function factor, there is now many researchs show polysaccharides have promote immune, anti-aging, it is antitumor, remove free radical,
The effects that antifatigue, radioresistance, liver protection, reproductive function protection and improvement.
Probiotics is a kind of by improving intestine microenvironment so as to the microorganism to host's generation beneficial effect.It can
Inhibit growth of pathogenic bacteria by participating in intestinal microecology balance, avoid the drug resistance of long-time service antibiotic generation and double
Infection, therefore, probiotic products receive the concern of people in recent years.
A kind of probiotics fermention functional food for improving male's inferior health of the present invention, had both saved the battalion of natural plants
Value, and the nutrition health-care functions with probiotics fermention product are supported, it is unique in taste;The probiotics fermention can improve male
The food that inferior health takes into account dispelling effects of alcohol and nourishing liver effect is prepared by specific process, by probiotics fermention, is made in natural plants
Beneficial activity ingredient, it is easier to absorption of human body, enhancing improve male's sub-health state and the effect of dispelling effects of alcohol and nourishing liver.By prebiotic
Bacterium fermentation improves the food and probiotics powder agent of male's inferior health and dispelling effects of alcohol and nourishing liver to prepare, and is ensureing that fermentation substrate is uniformly same
When, it is easy to accomplish large-scale production.
Specific embodiment
With reference to embodiment and the experimental example technical solution that the present invention will be described in detail, specific embodiment described herein
Be only used to explain the present invention, but the present invention is not limited to specific examples below and protection domain it is without being limited thereto.
The percentage sign " % " arrived involved in embodiment if not specified, refers to mass percent, and the percentage of solution refers to
Grams containing solute in 100mL, the percentage between liquid refer to the volume ratio of the solution at 25 DEG C.
Embodiment 1
A kind of preparation method for the probiotics fermention drinks for improving male's inferior health
1st, turmeric in raw material, sealwort and ratio of adjuvant determine
(1) by turmeric, black pepper, sealwort, black soya bean, raspberry, matrimony vine cleaning be placed on 60 DEG C of oven for drying 3-4 it is small when,
Then crush, be milled 100 mesh sieves respectively, obtains curcuma powder and black pepper, sealwort powder, black bean powder, raspberry powder, wolfberry fruit powder.
(2) activation medium is prepared:12g black bean powders are added in per 100ml water;It sterilizes to activation medium, condition
For, 121 DEG C, 20min;
(3) fermentation medium is prepared:2-8g curcuma powders, 2-8g sealwort powder are added in per 100ml water, in addition can add 1-5g
The wolfberry fruit powder of the raspberry powder and/or 1-3g of black pepper and/or 1-3g rape pollens and/or 1-3g black bean powders and/or 1-3g,
Obtain liquid state fermentation culture medium;
(4) actication of culture, lactobacillus plantarum, animal bifidobacteria, Lactobacillus paracasei, bifidobacterium adolescentis, condensation bud
The glycerol tube of spore bacillus and bacillus subtilis is inoculated in activation medium by 1% respectively and is activated, and pH is naturally, control temperature
For 37 DEG C;After activating for 24 hours respectively, by 10% (v/v) connect bacterium amount transfer again activation medium carry out re-activation, reactivation
Obtain secondary seed solution afterwards for 24 hours;
(5) probiotics fermention:It is 10 by the inoculum concentration of animal bifidobacteria6-107CFU/g, the inoculum concentration of lactobacillus plantarum
For 106-107CFU/g, the inoculum concentration of Lactobacillus paracasei is 105-106CFU/g, the inoculum concentration of bifidobacterium adolescentis is 106-
107CFU/g, the inoculum concentration of bacillus coagulans is 105-106CFU/g, the inoculum concentration of bacillus subtilis is 105-106CFU/g
It is inoculated in fermentation medium, lactobacillus plantarum and animal bifidobacteria, Lactobacillus paracasei, bifidobacterium adolescentis, condensation bud
Spore bacillus and bacillus subtilis connect bacterium amount volume ratio as 1:1:1:1:1:1, for 24 hours, temperature is 37 DEG C to mixed-strains liquid fermentation;Respectively
Number of live bacteria of probiotics in zymotic fluid is detected, as a result such as table 1:
Strain label:No. 1 is lactobacillus plantarum TK9 single bacteriums, and No. 2 are 379 single bacterium of animal bifidobacteria, and No. 3 are secondary cheese
Lactobacillus TK1501 single bacteriums, No. 4 are bifidobacterium adolescentis TK99 single bacteriums, and No. 5 are bacillus coagulans TQ33 single bacteriums, and No. 6 are withered
Careless bacillus TK-1 single bacteriums.
The present invention has carried out a variety of natural material mixes liquid fermentations, and curcuma powder, Huang are added according to different proportion per 100ml
Fine powder, black pepper, rape pollen, black bean powder, raspberry powder, wolfberry fruit powder carry out liquid state fermentation, and viable count the results are shown in Table 1.
Number of live bacteria of probiotics after the auxiliary material combination liquid state fermentation of 1 turmeric of table, sealwort and different proportion
|
Combination one |
Combination two |
Combination three |
Combination four |
Combination five |
No. 1 |
1.49×108 |
2.61×108 |
4.32×108 |
4.69×108 |
7.42×108 |
No. 2 |
1.09×107 |
2.12×108 |
3.06×108 |
4.37×108 |
8.08×108 |
No. 3 |
2.31×107 |
8.94×107 |
9.21×107 |
9.91×107 |
5.81×108 |
No. 4 |
3.45×106 |
1.82×107 |
3.71×107 |
5.72×107 |
8.91×107 |
No. 5 |
1.61×106 |
2.42×107 |
4.19×107 |
5.37×107 |
7.91×107 |
No. 6 |
1.12×105 |
2.57×106 |
4.49×106 |
6.69×106 |
8.61×106 |
Total viable count |
5.65×108 |
6.61×108 |
7.21×108 |
1.04×109 |
4.35×109 |
Each group is represented with label:Combination one:Turmeric:Sealwort:Black pepper:Rape pollen:Black soya bean:Raspberry:Matrimony vine=
6:4:3:3:2:2:3, combination two:Turmeric:Sealwort:Black pepper:Rape pollen:Black soya bean:Raspberry:Matrimony vine=2:4:3:2:2:1:
1, combination three:Turmeric:Sealwort:Black pepper:Rape pollen:Black soya bean:Raspberry:Matrimony vine=5:2:1:3:1:3:2, combination four:Ginger
It is yellow:Sealwort:Black pepper:Rape pollen:Black soya bean:Raspberry:Matrimony vine=5:4:5:1:3:1:2, combination five:Turmeric:Sealwort:Black Hu
Green pepper:Rape pollen:Black soya bean:Raspberry:Matrimony vine=8:8:4:3:2:1:1.
Curcuma powder is with after multiple auxiliary materials mixed fermentation, combining five as shown in Table 1:Turmeric:Black pepper:Sealwort:Rape pollen:
Black soya bean:Raspberry:Matrimony vine=8:8:5:3:2:1:1, i.e., often 8g curcuma powders, 8g sealworts powder, 4g black peppers are added in 100ml water
Viable count reaches highest when powder, 3g rape pollens, 2g black bean powders, 1g raspberry powders, 1g wolfberry fruit powders, and 4.35 × 109CFU/mL。
2nd, probiotics inoculum concentration is definite
Other conditions are same as above, by addition 8g curcuma powders, 8g sealworts powder, 4g black peppers, 3g rape flowers in every 100ml water
Powder, 2g black bean powders, 1g raspberry powders, 1g wolfberry fruit powders prepare fermentation medium, by lactobacillus plantarum TK9 by it is following connect bacterium amount 1 ×
106、5×106、1×107、5×107CFU/mL is inoculated in fermentation medium respectively, the ratio of other strains and lactobacillus plantarum
Under such as:Lactobacillus plantarum TK9, animal bifidobacteria 937, Lactobacillus paracasei TK1501, bifidobacterium adolescentis TK99, condensation
Bacillus TQ33, the bacterium amount ratio that connects of bacillus subtilis TK-1 are 1:1:1:1:1:1,37 DEG C of fermentation, fermentation are surveyed after completing
Determine the number of live bacteria of probiotics in zymotic fluid.
Viable count (CFU/mL) under 2 different vaccination amount of table
The results are shown in Table 2:Optimum inoculation amount lactobacillus plantarum TK9, animal bifidobacteria 937, Lactobacillus paracasei
TK1501, bifidobacterium adolescentis TK99, bacillus coagulans TQ33, bacillus subtilis TK-1 are 1 × 107CFU/mL.It surveys
The total viable count obtained is 5.43 × 109CFU/mL。
3rd, incubation time is definite
It is yellow that 8g curcuma powders, 8g are added under conditions of inoculum concentration has optimized, in the every 100ml water of liquid state fermentation culture medium selection
Fine powder, 4g black peppers, 3g rape pollens, 2g black bean powders, 1g raspberry powders, 1g wolfberry fruit powders.
It is lactobacillus plantarum TK9, animal bifidobacteria 937, Lactobacillus paracasei TK1501, bifidobacterium adolescentis TK99, solidifying
Knot bacillus TQ33, the glycerol tube of bacillus subtilis TK-1 are inoculated in black bean powder activation medium by 1% and are once lived
Change, activate for 24 hours, then transfer by the inoculum concentration of 10% (v/v) in re-activation culture medium, re-activation is for 24 hours.In liquid
Inoculated plant lactobacillus TK9, animal bifidobacteria 937, Lactobacillus paracasei TK1501, youth bifid bar in state fermentation medium
Bacterium TK99, bacillus coagulans TQ33, bacillus subtilis TK-1 seed liquor mixed fungus fermentations.So that it is linked into turmeric liquid state fermentation
It is lactobacillus plantarum TK9 in culture medium, animal bifidobacteria 937, Lactobacillus paracasei TK1501, bifidobacterium adolescentis TK99, solidifying
Bacillus TQ33, bacillus subtilis TK-1 are tied, is 1 × 107CFU/mL;PH is naturally, 37 DEG C of cultivation temperature, mixed fungus fermentation
12nd, 24,36,48h, is respectively detected the number of live bacteria of probiotics in zymotic fluid.
Influence of the 3 different fermentations time of table to number of live bacteria of probiotics after culture
Strain label:No. 1 is lactobacillus plantarum TK9 single bacteriums, and No. 2 are 379 single bacterium of animal bifidobacteria, and No. 3 are secondary cheese
Lactobacillus TK1501 single bacteriums, No. 4 are bifidobacterium adolescentis TK99 single bacteriums, and No. 5 are bacillus coagulans TQ33 single bacteriums, and No. 6 are withered
Careless bacillus TK-1 single bacteriums.
|
12h |
24h |
36h |
48h |
No. 1 |
4.86×108 |
5.19×108 |
3.77×108 |
3.94×108 |
No. 2 |
5.09×108 |
5.29×108 |
2.21×108 |
6.36×108 |
No. 3 |
9.81×107 |
7.37×107 |
6.71×107 |
8.45×107 |
No. 4 |
8.15×107 |
6.47×107 |
5.17×107 |
7.49×107 |
No. 5 |
6.31×107 |
7.69×107 |
2.57×107 |
6.01×107 |
No. 6 |
6.39×105 |
8.51×105 |
3.98×105 |
7.41×105 |
Total viable count |
7.35×108 |
9.01×109 |
5.31×109 |
4.71×109 |
As a result shown in table 3:The optimal incubation time of mixed-strains liquid fermentation is for 24 hours, viable count is 9.01 × 109CFU/ml。
4th, a kind of preparation method for the liquid state fermentation beverage for improving male's inferior health
(1) will be dried after turmeric and auxiliary material cleaning, 100 mesh sieves that were milled respectively obtain curcuma powder and various auxiliary material powder;
(2) activation medium is prepared:12g black bean powders are added in per 100ml water;It sterilizes to activation medium, condition
For, 121 DEG C, 20min;
(3) fermentation medium is prepared:8g curcuma powders, 8g sealworts powder, 4g black peppers, 3g rapes are added in per 100ml water
Pollen, 2g black bean powders, 1g raspberry powders, 1g wolfberry fruit powders;
(4) actication of culture, lactobacillus plantarum, animal bifidobacteria, Lactobacillus paracasei TK1501, bifidobacterium adolescentis
TK99, bacillus coagulans TQ33, bacillus subtilis TK-1 glycerol tubes are inoculated in activation medium by 1% respectively and live
Change, pH is naturally, controlled at 37 DEG C;After activating for 24 hours respectively, transfer again activation medium by the bacterium amount that connects of 10% (v/v)
Re-activation is carried out, reactivation obtains secondary seed solution afterwards for 24 hours;
(5) probiotics fermention:By lactobacillus plantarum by connecing bacterium amount as 1 × 107CFU/mL is inoculated in fermentation medium, together
When be inoculated with animal bifidobacteria, Lactobacillus paracasei TK1501, bifidobacterium adolescentis TK99, bacillus coagulans TQ33, withered grass
Bacillus TK-1, lactobacillus plantarum, animal bifidobacteria, Lactobacillus paracasei TK1501, bifidobacterium adolescentis TK99, condensation
Bacillus TQ33, bacillus subtilis TK-1 connect bacterium amount volume ratio as 1:1:1:1:1:1, mixed-strains liquid fermentation for 24 hours, temperature
For 37 DEG C of fermentations;
(6) zymotic fluid is after emulsification after fermentation plus stabilizer, stabilizer press the quality percent by volume with zymotic fluid
Be added, additive amount be sodium carboxymethylcellulose 0.5%, xanthans 0.6%, emulsifier 0.8%;Add zymotic fluid mass body
The different wheat ketose that product percentage is 8% carries out adjusting sour blending;At 65 DEG C, homogeneous once, is sterilized through 80 DEG C under conditions of 30MPa
15-20min obtains the probiotic fermentation beverage that can improve male's inferior health, which has dispelling effects of alcohol and nourishing liver effect concurrently.
5th, culture medium active ingredient changes before and after liquid-state fermentation technology-fermentation
It is lactobacillus plantarum TK9, animal bifidobacteria 937, Lactobacillus paracasei TK1501, bifidobacterium adolescentis TK99, solidifying
Knot bacillus TQ33, bacillus subtilis TK-1 glycerol tubes are inoculated in black bean powder liquid culture medium by 1% respectively and carry out once
Activation, activates for 24 hours, then transfers by the bacterium amount that connects of 10% (v/w) into re-activation culture medium (with an activation culture
Base) in, re-activation for 24 hours, with turmeric:Black pepper:Sealwort:Rape pollen:Black soya bean:Raspberry:Matrimony vine=8:8:4:3:2:1:1
Liquid state fermentation culture medium, and inoculated plant lactobacillus TK9, animal bifidobacteria 937, Lactobacillus paracasei simultaneously is made
TK1501, bifidobacterium adolescentis TK99, bacillus coagulans TQ33, bacillus subtilis TK-1 seed liquors, inoculum concentration difference are equal
For 1 × 107CFU/mL, at 37 DEG C, pH naturally, mixed fungus fermentation for 24 hours.Curcumin is measured using GB/T 223.75-2008 methods;Profit
With Syrups by HPLC GABA content;Utilize the total phenols content of forint phenol reaction assay;It is surveyed using GB/T 23788-2009 methods
Determine Flavone content;The content of amino nitrogen is measured using GB/T12143.2-89 methods;It is measured using ozone index method (ORAC)
Polyphenoils content, the results are shown in Table 4:
Active ingredient changes before and after the functional beverage fermentation of the improvement male's inferior health of table 4
Each stage is represented with number:Content before 1- fermentations;Content after 2- mixed fungus fermentations;After 3- lactobacillus plantarums TK9 fermentations
Content;Content after 4- animal bifidobacterias 937 ferment;Content after 5- Lactobacillus paracaseis TK1501 fermentations;6- youth bifid bars
Content after bacterium TK99 fermentations;Content after 7- bacillus coagulanses TQ33 fermentations;Content after 8- bacillus subtilises TK-1 fermentations
From data above, curcuma powder, black pepper, sealwort powder, rape pollen, black bean powder, raspberry powder, wolfberry fruit powder
After prebiotic strains liquid fermentation active ingredient be all improved.Especially through mixed fungus fermentation, content reaches curcumin after fermentation
8.81mg/g;GABA content increased after fermented, and liquid beverage GABA content is reached for 4.11mg/g after mixed fungus fermentation,
It is above the single bacterium fermentation total phenols contents of and reaches 9.72mg GAE/g;After mixed fungus fermentation, Flavonoid substances content substantially increases
Add, content reaches 1.91mg/g, and amino-acid nitrogen content increases before relatively fermenting.Mixed fungus fermentation sample is compared with unfermentable fermentation
It liquid and is fermented using single bacterium, DPpH inhibiting rates have reached 47%.
Embodiment 2:The probiotics solid state fermentation food making method of male's inferior health can be improved
1st, raw material proportioning is definite
(1) dry, be milled after being respectively washed turmeric, black pepper, sealwort, rape pollen, black soya bean, raspberry, matrimony vine
100 mesh sieves.
(2) activation medium is prepared:12g black bean powders are added in per 100ml water;It sterilizes to activation medium, condition
For, 121 DEG C, 20min;
(3) fermentation medium is prepared:It chooses curcuma powder, sealwort powder and black pepper, rape pollen, black bean powder, cover basin
Sub- powder, wolfberry fruit powder one of which or several by certain weight ratio mixing, obtain the mixture of turmeric, sealwort and auxiliary material:Water=
1:Solid-state fermentation culture medium is made in 0.8-2;The mixture of the turmeric, sealwort and auxiliary material:The ratio of water is weight ratio;
(4) actication of culture, lactobacillus plantarum, animal bifidobacteria, Lactobacillus paracasei, bifidobacterium adolescentis, condensation bud
The glycerol tube of spore bacillus and bacillus subtilis is inoculated in activation medium by 1% respectively and is activated, and pH is naturally, control temperature
For 37 DEG C;After activating for 24 hours respectively, by 10% (v/v) connect bacterium amount transfer again activation medium carry out re-activation, reactivation
Obtain secondary seed solution afterwards for 24 hours;
(5) probiotics fermention:It is 10 by the inoculum concentration of animal bifidobacteria6-107CFU/g, the inoculum concentration of lactobacillus plantarum
For 106-107CFU/g, the inoculum concentration of Lactobacillus paracasei is 105-106CFU/g, the inoculum concentration of bifidobacterium adolescentis is 106-
107CFU/g, the inoculum concentration of bacillus coagulans is 105-106CFU/g, the inoculum concentration of bacillus subtilis is 105-106CFU/g
It is inoculated in fermentation medium;Lactobacillus plantarum and animal bifidobacteria, Lactobacillus paracasei, bifidobacterium adolescentis, condensation bud
Spore bacillus and bacillus subtilis connect bacterium amount volume ratio as 1:1:1:1:1:1, for 24 hours, temperature is 37 DEG C to mixed culture solid state fermentation;Respectively
Number of live bacteria of probiotics in fermented sample is detected, as a result such as table 5:
Strain label:No. 1 is lactobacillus plantarum TK9 single bacteriums, and No. 2 are 379 single bacterium of animal bifidobacteria, and No. 3 are secondary cheese
Lactobacillus TK1501 single bacteriums, No. 4 are bifidobacterium adolescentis TK99 single bacteriums, and No. 5 are bacillus coagulans TQ33 single bacteriums, and No. 6 are withered
Careless bacillus TK-1 single bacteriums
Raw material chooses turmeric, sealwort, black pepper, rape pollen, black soya bean, raspberry, matrimony vine, raw material weight proportioning of soup processed label:
Combination one:4:4:2:1:1:1:1, combination two:5:4:3:1:1:1:1, combination three:5:4:4:2:1:1:1, combination four:5:3:3:
2:2:1:1, combination five:5:5:4:2:1:1:1, combination six:3:4:1:2:2:1:1, combination seven:3:3:1:2:1:1:2, combination
Eight:4:3:2:2:1:1:1, combination nine:4:3:1:2:2:1:1.
Number of live bacteria of probiotics after 5 curcuma powder of table, sealwort powder and auxiliary material different ratio solid state fermentation
|
Combination one |
Combination two |
Combination three |
Combination four |
Combination five |
Combination six |
Combination seven |
Combination eight |
Combination nine |
No. 1 |
2.51×108 |
3.51×108 |
4.23×108 |
6.65×108 |
6.81×108 |
7.49×108 |
5.34×108 |
4.82×108 |
7.56×108 |
No. 2 |
2.77×107 |
4.38×107 |
7.54×107 |
8.76×107 |
7.67×108 |
2.64×108 |
6.56×108 |
4.67×108 |
3.13×108 |
No. 3 |
7.31×107 |
8.36×107 |
9.87×107 |
4.76×108 |
7.32×108 |
5.34×108 |
4.01×108 |
3.17×108 |
2.19×108 |
No. 4 |
1.42×107 |
8.27×107 |
5.75×108 |
7.88×108 |
9.61×108 |
6.36×108 |
3.69×108 |
4.16×108 |
3.65×108 |
No. 5 |
2.01×107 |
4.68×107 |
5.26×107 |
7.38×107 |
6.84×108 |
5.25×108 |
4.15×108 |
3.19×108 |
1.86×108 |
No. 6 |
3.98×105 |
8.01×105 |
9.38×105 |
3.26×107 |
5.74×107 |
7.91×107 |
3.16×107 |
4.27×107 |
3.79×107 |
Total viable count |
1.87×1010 |
8.26×109 |
7.26×108 |
2.17×109 |
3.71×1010 |
8.06×109 |
9.13×109 |
4.74×108 |
2.41×108 |
The optimal proportion obtained as shown in Table 5 is curcuma powder:Sealwort powder:Black pepper:Rape pollen:Black bean powder:Cover basin
Sub- powder:Wolfberry fruit powder=5:5:4:2:1:1:1, total viable count is 3.71 × 1010CFU/mL。
2nd, strain inoculum concentration is definite
It is lactobacillus plantarum TK9, animal bifidobacteria 937, Lactobacillus paracasei TK1501, bifidobacterium adolescentis TK99, solidifying
Knot bacillus TQ33, bacillus subtilis TK-1 glycerol tubes are inoculated in activation medium by 1% respectively and (add in per 100ml water
Add 12g black bean powders, sterilising conditions are 121 DEG C, 20min;) once activated;It activates for 24 hours, then presses 10% (v/w)
The bacterium amount that connects transfer in re-activation culture medium (with an activation medium), re-activation for 24 hours, by curcuma powder:Sealwort powder:
Black pepper:Rape pollen:Black bean powder:Raspberry powder:Wolfberry fruit powder=5:5:4:2:1:1:1, material-water ratio 1:1.2 are made solid-state
Fermentation medium, while inoculated plant lactobacillus TK9, animal bifidobacteria 937, Lactobacillus paracasei TK1501, youth bifid
Bacillus TK99, bacillus coagulans TQ33, bacillus subtilis TK-1 seed liquors carry out mixed fungus fermentation, mixed culture solid state fermentation for 24 hours,
37 DEG C, pH is natural.Number of live bacteria of probiotics in fermentation wild Oryza species is detected.
Inoculum concentration:By lactobacillus plantarum TK9 (L.plantarum TK9), 937 (B.animalis of animal bifidobacteria
937), Lactobacillus paracasei TK1501 (Lactobacillus paracaseiTK1501), bifidobacterium adolescentis TK99
(Bifidobacterium adolescentisTK99), bacillus coagulans TQ33 (Bacillus coagulansTQ33),
Bacillus subtilis TK-1 (Bacillus subtilisTK-1) while solid-state fermentation culture medium is accessed, inoculum concentration is:1×
106CFU/mL;5×106CFU/mL;1×107CFU/mL;5×107Tetra- groups of CFU/mL, the results are shown in Table 6:
Viable count (CFU/g) under 6 solid state fermentation different vaccination amount of table
The results are shown in Table 6:Lactobacillus plantarum TK9 (L.plantarum TK9), animal bifidobacteria 937
(B.animalis937), Lactobacillus paracasei TK1501 (Lactobacillus paracaseiTK1501), youth bifid bar
Bacterium TK99 (Bifidobacterium adolescentisTK99), bacillus coagulans TQ33 (Bacillus
CoagulansTQ33), the optimum inoculation amount of bacillus subtilis TK-1 (Bacillus subtilisTK-1) for 5 ×
107CFU/g, that is, measure gained improve total viable count in the probiotics fermention functional food of male's inferior health for 9.67 ×
1010CFU/g.Wherein, lactobacillus plantarum viable count is 7.11 × 109CFU/g, the viable count of animal bifidobacteria reaches 5.15 ×
108CFU/g, Lactobacillus paracasei viable count are 2.37 × 108CFU/g, bifidobacterium adolescentis viable count are 5.69 × 108CFU/
G, coagulated bacillus living number are 8.41 × 107CFU/g, the viable count of bacillus subtilis is 3.62 × 106CFU/g。
3rd, material-water ratio is definite
Solid-state fermentation culture medium:The ratio of raw material and water (mass volume ratio) is respectively 1:0.8,1:1,1:1.2,1:1.6
1:1.8,1:2.
It is lactobacillus plantarum TK9, animal bifidobacteria 937, Lactobacillus paracasei TK1501, bifidobacterium adolescentis TK99, solidifying
Knot bacillus TQ33, bacillus subtilis TK-1 glycerol tubes are inoculated in black bean powder liquid culture medium by 1% respectively and carry out once
Activation, activates for 24 hours, then transfers by the bacterium amount that connects of 10% (v/w) into re-activation culture medium (with an activation culture
Base) in, re-activation for 24 hours, with curcuma powder:Sealwort powder:Black pepper:Rape pollen:Black bean powder:Raspberry powder:Wolfberry fruit powder=
5:5:4:2:1:1:1 is made solid-state fermentation culture medium, and inoculated plant lactobacillus TK9, animal bifidobacteria 937, pair are dry simultaneously
Lactobacillus paracasei TK1501, bifidobacterium adolescentis TK99, bacillus coagulans TQ33, bacillus subtilis TK-1 seed liquors, inoculation
Amount is 5 × 10 respectively7CFU/mL carries out mixed fungus fermentation.Condition of culture:It is closed for 24 hours, 37 DEG C, pH is natural.To being cultivated after fermentation
Number of live bacteria of probiotics in base is detected, and the results are shown in Table 7:
The proportioning of 7 curcuma powder of table and water is 1:During 0.8-2, number of live bacteria of probiotics (CFU/g) after fermentation
The results are shown in Table 7:Optimal material-water ratio is 1:1.2, that is, it measures in gained turmeric probiotics fermention functional food
Total viable count be 5.79 × 109CFU/g.Wherein, lactobacillus plantarum viable count is 8.71 × 108CFU/g, animal bifidobacteria
Viable count reach 4.37 × 108CFU/g, the viable count of Lactobacillus paracasei is 5.11 × 108CFU/g, bifidobacterium adolescentis
Viable count is 6.27 × 108CFU/g, the viable count of bacillus coagulans is 3.15 × 108CFU/g, the viable bacteria of bacillus subtilis
Number is 7.29 × 107CFU/g。
4th, incubation time is definite
It is lactobacillus plantarum TK9, animal bifidobacteria 937, Lactobacillus paracasei TK1501, bifidobacterium adolescentis TK99, solidifying
Knot bacillus TQ33, bacillus subtilis TK-1 glycerol tubes are inoculated in black bean powder liquid culture medium by 1% respectively and carry out once
Activation, activates for 24 hours, then transfers by the bacterium amount that connects of 10% (v/w) into re-activation culture medium (with an activation culture
Base) in, re-activation for 24 hours, the inoculated plant lactobacillus TK9, dynamic simultaneously in the optimized solid-state fermentation culture medium of material-water ratio
Object Bifidobacterium 937, Lactobacillus paracasei TK1501, bifidobacterium adolescentis TK99, bacillus coagulans TQ33, bacillus subtilis
Bacterium TK-1 seed liquors, inoculum concentration are 5 × 10 respectively7CFU/g carries out mixed fungus fermentation.Mix respectively bacterium sealed fermenting 12,24,36,
48h, 37 DEG C, pH is natural.Number of live bacteria of probiotics in fermentation wild Oryza species is detected.
Probiotics viable bacteria after the culture of 8 different fermentations time of table
Strain label:No. 1 is lactobacillus plantarum TK9 single bacteriums, and No. 2 are 379 single bacterium of animal bifidobacteria, and No. 3 are secondary cheese
Lactobacillus TK1501 single bacteriums, No. 4 are bifidobacterium adolescentis TK99 single bacteriums, and No. 5 are bacillus coagulans TQ33 single bacteriums, and No. 6 are withered
Careless bacillus TK-1 single bacteriums.
|
12h |
24h |
36h |
48h |
No. 1 |
3.26×108 |
4.89×108 |
6.54×108 |
3.75×108 |
No. 2 |
4.65×108 |
2.61×108 |
5.16×108 |
2.67×108 |
No. 3 |
5.61×107 |
8.92×107 |
4.66×108 |
2.15×108 |
No. 4 |
8.45×107 |
9.51×107 |
2.71×108 |
1.07×108 |
No. 5 |
6.73×106 |
1.07×107 |
2.59×107 |
8.65×106 |
No. 6 |
2.72×105 |
3.26×105 |
4.75×105 |
4.19×105 |
Total viable count |
5.56×109 |
5.11×1010 |
8.01×1010 |
4.78×109 |
The results are shown in Table 8:Optimal incubation time is mixed fungus fermentation 36h again after inoculation, total viable count for 8.01 ×
1010CFU/g。
5th, culture medium active ingredient changes before and after solid-state fermentation process-fermentation
It is lactobacillus plantarum TK9, animal bifidobacteria 937, Lactobacillus paracasei TK1501, bifidobacterium adolescentis TK99, solidifying
Knot bacillus TQ33, bacillus subtilis TK-1 glycerol tubes are inoculated in black bean powder liquid culture medium by 1% respectively and carry out once
Activation, activates for 24 hours, then transfers by the bacterium amount that connects of 10% (v/w) into re-activation culture medium (with an activation culture
Base) in, re-activation for 24 hours, with curcuma powder:Sealwort powder:Black pepper:Rape pollen:Black bean powder:Raspberry powder:Wolfberry fruit powder=
5:5:4:2:1:1:1, material-water ratio 1:1.2 are made solid-state fermentation culture medium, and inoculated plant lactobacillus TK9, animal bifid simultaneously
Bacillus 937, Lactobacillus paracasei TK1501, bifidobacterium adolescentis TK99, bacillus coagulans TQ33, bacillus subtilis TK-1
Seed liquor, inoculum concentration are 5 × 10 respectively7CFU/mL, at 37 DEG C, pH is naturally, mixed fungus fermentation 36h.Utilize GB/T223.75-
2008 methods measure curcumin;Utilize Syrups by HPLC GABA content;Utilize the total phenols content of forint phenol reaction assay;It utilizes
GB/T 23788-2009 methods measure Flavone content;The content of amino nitrogen is measured using GB/T12143.2-89 methods;Utilize work
Oxygen index method (ORAC) measures polyphenoils content, the results are shown in Table 9:
Active ingredient changes before and after the solid-state food fermentation of the improvement male's inferior health of table 9
Each stage is represented with number:Content before 1- fermentations;Content after 2- mixed fungus fermentations;After 3- lactobacillus plantarums TK9 fermentations
Content;Content after 4- animal bifidobacterias 937 ferment;Content after 5- Lactobacillus paracaseis TK1501 fermentations;6- youth bifid bars
Content after bacterium TK99 fermentations;Content after 7- bacillus coagulanses TQ33 fermentations;Content after 8- bacillus subtilises TK-1 fermentations
From data above, curcuma powder, black pepper, sealwort powder, rape pollen, black bean powder, raspberry powder, wolfberry fruit powder
After probiotics mixed culture solid state fermentation active ingredient be all improved.Content reaches 9.97mg/g to curcumin after fermentation;
GABA content increased after fermented, and GABA content is reached for 5.25mg/g after mixed fungus fermentation, and it is total to be above single bacterium fermentation
Phenols content reaches 10.61mg GAE/g;After mixed fungus fermentation, Flavonoid substances content substantially increases, and content reaches 2.35mg/
G, amino-acid nitrogen content increase before relatively fermenting.Mixed fungus fermentation sample is fermented compared with non-fermentation medium and using single bacterium,
Its DPpH inhibiting rate has reached 57%.
3 prebiotic strains liquid fermentation beverage of embodiment and solid state fermentation beverage animal kidney tonifying experiment
Zoopery is carried out to prebiotic strains liquid fermentation beverage and solid beverage, and gavages physiological saline with control group and carries out
Comparison.Experimental method is as follows:
Experimental animal:Wistar male white rats 80 of 1 month from breast, weight 150-180g.
Tested material dosage:5g/ only, continuously takes 20 days.
Experimental method:90 rats are randomly divided into prebiotic strains liquid fermentation beverage and tested group of solid beverage and control
Group, every group 30, by the rat of prebiotic tested group one of strains liquid fermentation beverage feeding, by tested group of probiotics solid beverage feeding
Two rat, control group:By wherein 30 similary rearing conditions of rat, normal saline is gavaged.Test method is with reference to China
The telotism test method of Xiyuan hospital of the academy of traditional Chinese medicine carries out, and records eclipse period of penile erection, and carries out statistics between group
Analysis.Result of the test is shown in Table 10:
10 prebiotic strains liquid fermentation beverage of table and solid state fermentation beverage are to animal kidney tonifying experimental result
The experimental results showed that a certain amount of prebiotic strains liquid fermentation beverage provided by the present invention and solid beverage can improve
Castrated rats penis is to the excitability of outside stimulus, and under local micro electro photoluminescence effect, rat eclipse period of penile erection is apparent
Shorten, compared with the control group, there is apparent strengthening yang and invigorating kidney.
4 prebiotic strains liquid fermentation beverage of embodiment and the research of the high male sexual function effect of solid state fermentation beverage lifting
With male mice, weight 25-28g is random to be grouped, experimental group every group 10, control group 10.Experimental group fills respectively
The liquid and solid state fermentation beverage 5g/ day of the preparation of embodiment 1,2 are taken, control group gavages normal saline, one time a day, persistently
24 days.From the 4th day, each group animal daily when 25 DEG C of -30 DEG C of deep water middle reaches leaching 3.5 is small as stress, continue three weeks.It is taking
With one week and take the 25th and do sexual function experiment.
Female adult mouse, weight 25-30g.To increase the Oestrus rate of animal, intramuscular injection female hormone diethylstilbestrol
After 0.4mg/kg/ days, 2 days, vaginal smear microscopy using the seedless keratinocyte in surface layer as main person, shows, in estrus, to record
With.This heat female mice, 5 cage packing.In 6 points of early morning, the male mice of an experiment is added per cage, is observed in 5 minutes
The number of copulations and mating rate of generation.Specific experiment the results are shown in Table 11:
The influence of 11 prebiotic strains liquid fermentation beverage of table and solid state fermentation beverage to mouse sexual function
It gavages as shown in Table 11 after a week, experimental group mates frequency apparently higher than control group mating frequency, there is conspicuousness meaning
Justice, experimental group mating rate are also higher than control group mating rate.It gavages the 25th, the sexual function of control animals is decreased obviously, and real
The mating rate of group animal is tested without change, only mating frequency number is reduced, and experimental group mating frequency mates apparently higher than control group
Frequency, there is significant, and experimental group mating rate is also higher than control group mating rate, there is significant.
From table 10,11 data of table, prebiotic strains liquid fermentation beverage and solid state fermentation beverage energy provided by the present invention
Enough shorten the eclipse period of penile erection of rat, improve mouse sexual function, show that this product can effectively adjust animal bodies function,
Tonifying kidney and strengthening yang improves male's inferior health.
5 prebiotic strains liquid fermentation beverage of embodiment and the experiment of solid state fermentation beverage animal relieving alcoholism and protecting liver
Zoopery is carried out to prebiotic strains liquid fermentation beverage and solid beverage, and gavages physiological saline with control group and carries out
Comparison.Experimental method is as follows:
Experimental animal:Wistar male white rats 80 of 1 month from breast, weight 150-180g.
Experimental method:30 rats are randomly divided into three groups, every group 10, gavage 56 ° of white wine 3g daily, experimental group 1 is same
When gavage prebiotic strains liquid fermentation beverage 5g, experimental group 2 gavages probiotics solid beverage 5g simultaneously, and control group gavages 5g lifes simultaneously
Brine is managed, once a day, after gavaging six weeks, fasting 12h, broken end takes blood, and after standing 2h, centrifugation separates serum, -80 DEG C of storages
It is spare.Serum aspartate amino transferase, alanine aminotransferase detection are carried out by the specification operation of kit.Tool
Body the results are shown in Table 12:
Table 12 each group mice serum AST, ALT compare (X ± S)
Group |
AST(U/L) |
ALT(U/L) |
Experimental group 1 |
71.67±7.22 |
50.67±1.58 |
Experimental group 2 |
72.56±6.35 |
51.42±1.77 |
Control group |
83.32±6.18 |
58.25±2.59 |
Through animal mouse experiment, probiotics liquid beverage provided by the present invention can be reduced significantly with solid beverage
Blood AST, ALT of mouse are horizontal, have and significantly relieve the effect of alcohol, protect liver cell, mitigate effect of the alcohol to hepatocellular injury.
Embodiment described above only expresses the several embodiments of the present invention, and description is more specific and detailed, but simultaneously
Cannot the limitation to the scope of the claims therefore be interpreted as.It should be pointed out that for those of ordinary skill in the art,
On the premise of not departing from this patent design, the respective embodiments described above can also make several deformations, combination and improve, these all belong to
In the protection domain of this patent.Therefore, the protection domain of this patent should be determined by the appended claims.