CN108094792A

CN108094792A - A kind of probiotics fermention functional food for improving male's inferior health and preparation method thereof

Info

Publication number: CN108094792A
Application number: CN201711440130.7A
Authority: CN
Inventors: 王海宽; 张震; 王伟
Original assignee: Tianjin University of Science and Technology
Current assignee: Tianjin Chuang Yuan Biotechnology Co Ltd
Priority date: 2017-12-27
Filing date: 2017-12-27
Publication date: 2018-06-01

Abstract

The invention belongs to microbial fermentation application field, relate to the use of the functional food of improvement male's sub-health state of the productions such as probiotics fermention turmeric, sealwort, black pepper, which has effects that dispelling effects of alcohol and nourishing liver simultaneously.It is for fermentation substrate, using lactobacillus plantarum CGMCC No.11891 with turmeric, sealwort and black pepper, rape pollen, black soya bean, raspberry, matrimony vine etc.；Animal bifidobacteria CGMCC No.11892, Lactobacillus paracasei TK1501, CGMCC No.13130, bifidobacterium adolescentis TK99, CGMCC No.12784, bacillus coagulans TQ33, CGMCC No.5233, bacillus subtilis TK 1, CGMCC No.4731 are prepared after liquid or solid-state mixed fungus fermentation.By probiotics fermention, make the beneficial activity ingredient in natural plants, it is easier to which absorption of human body, enhancing improve male's sub-health state and the effect of dispelling effects of alcohol and nourishing liver.

Description

A kind of probiotics fermention functional food for improving male's inferior health and preparation method thereof

Technical field

The invention belongs to microbial fermentations to produce field of functional food, relates to the use of probiotics fermention turmeric, sealwort, black Pepper, rape pollen, black soya bean, raspberry, matrimony vine produce the preparation method of functional food and functional food, the work( Energy property food is mainly used for improving male's sub-health state, including improving male prostate problem and sexual function, the functionality Food has effects that dispelling effects of alcohol and nourishing liver simultaneously.

Background technology

As society develops rapidly, world today's competition is increasing, and thing followed life increasingly increases with operating pressure It is long, many people, especially male is caused to be in sub-health state, and then many males is caused different degrees of prostate occur and are asked Topic and sex dysfunction.Prostate is the distinctive sexual glands of male, and prostate problems can not only cause some local symptoms, Such as frequent micturition, urgent urination, urethra cusalgia are more likely to result in the change of other functions of body, such as, influence sexual function, fertility, interior Dyssecretosis causes infection, insanity, forms hyperplasia, causes canceration etc..Male sexual disfunction refer to male's sexual and Sexual satisfaction is incompetent, according to the literature, Chinese male sex dysfunction illness rate, between 40-49 Sui, illness rate 32%, 50-59 Illness rate is up to 74% between illness rate is 36%, 60-69 Sui between year.In addition, China consumes big country, mistake as a drinks The phenomenon that amount is drunk is not uncommon for, and ethyl alcohol has direct toxic action to the histoorgan of human body in wine, the device most sensitive to ethyl alcohol Official is liver.Continuous excessive consumption of alcohol energy loss impariment of the liver cell, disturbs the eubolism of liver, and then alcoholic hepatitis and liver can be caused hard Change.

It is short on the drug or effective time that improve male prostate problem and sex dysfunction on the market and aggravate liver kidney Burden or side effect are big, may damage body, aggravate the state of an illness, there are very big hidden danger.And current relieving alcoholism and protecting liver product both at home and abroad is such as Diuresis class chemical synthetic drug, liver-protecting and stomach-nourishing class Chinese medicine, albumen peptide medicament, more side effects are apparent or slowly effect, therapeutic effect The bad or mechanism of action is indefinite, and there are security risks.Turmeric, black pepper, sealwort, rape pollen, black soya bean, raspberry, matrimony vine Plants is waited just to play the role of supplementing the kidney to control the nocturnal, boosting qi and nourishing yin in traditional Chinese medicine system, qi-restoratives strengthening the essence, nourish the liver to improve visual acuity, in recent years, section Learn research and also indicate that more than plant is improving male's inferior health, anti-prostatic hyperplasia and tumor of prostate, improve male's sexual, Sexual deterioration, dispelling effects of alcohol and nourishing liver etc. is delayed to have good effect, currently also has and is much changed using natural plant composition Kind male's sub-health state, improves male's sexual and prostate problems, the patent of dispelling effects of alcohol and nourishing liver：

Publication number：The invention of CN105456522A《A kind of composition for improving male's sexual and application》Disclose one kind The composition and application, its composition for improving male's sexual are to form Fructus Rubi extract 15-30 by following components by weight Part, 15-30 parts of oyster water extract, 10-20 parts of Fructus lycii P.E, 10-20 parts of grapefruit powder, 10-20 parts of seabuckthorn fruit powder, ginseng carry Take 1-10 parts of object, 1-10 parts of Maca extract.

Publication number：The invention of CN106390004A《A kind of the treatment of sexual dysfunction, the composition of prostatic disorders and its system Preparation Method》A kind of the treatment of sexual dysfunction, the composition and preparation method thereof of prostatic disorders are disclosed, the composition includes The following mass fraction formula components fruit of Chinese wolfberry, dogwood fruit, cultivated land, Radix Angelicae Sinensis, Cortex Eucommiae, rhizome of chuanxiong, Radix Codonopsis, sealwort, Morinda officinalis, thizoma curculiginis, The fruit of Cherokee rose, psoralea corylifolia, Herba Epimedii, Schisandra chinensis, Semen Cuscutae, Chinese cassia tree, Gorgon fruit, rhizoma cibotii, frutus cnidii, fructus alpiniae oxyphyllae, Herba Cistanches, Radix Glycyrrhizae, It is Radix Astragali, the fruit of glossy privet, mulberries, mulberry Piao sheaths, cardamom, flower bud perfume, dried orange peel, sea Piao sheath, actinolite, felwort, rhizoma alismatis, Ramulus Taxilli, floating small Wheat, hedgehog skin, Du's root bark of tree peony, acorus graminens soland and asarum.To there is the drug matching of nourishing kidney yin, warming and invigorating kidney Yang, pass through conjunction Reason adjusts the proportioning of each formula, there is broad-spectrum action, without side-effects, curative effect to show the treatment of sexual dysfunction and prostatic disorders Work, healing cycle are short.

Publication number：The invention of CN102233086A《A kind of health care Chinese medicine for improving inferior health and improving male's energy》It is open A kind of improvement inferior health improves male's essence, gas, the health care Chinese medicine of god.It is with sea-buckthorn, flower of Panax ginseng, Cortex Eucommiae, Schisandra chinensis, fragrant-flowered garlic Vegetable seeds, the fruit of Chinese wolfberry, Herba Epimedii, Semen Cuscutae, the fruit of Cherokee rose, Fructus Corni, wilsonii, Herba Cistanches, Bostrychia montagnei, dried orange peel, oolong tea, sweetleaf Chrysanthemum forms.The effect of with supplementing male sex hormone, delaying male sex hormone early ageing.

The invention of open (bulletin) number CN102579703A《A kind of improvement male's sexual product and preparation method thereof》It is public A kind of improvement male's sexual product and preparation method thereof is opened, raw material includes following components and parts by weight content：Cordyceps sinensis is extracted Object 10-20, tribulus fruit extract 10-20, grape extract 20-30, Pulp Citrulli extract 5-15, sea cucumber extract 8-15, rhodiola root Extract 5-15, HERBA EPIMEDII extract 10-20.Compared with prior art, the present invention is combined with traditional Chinese and western medicine theory, under improvement It is studied based on hypothalamic-pituitary-gonad index, strengthens excited adjusting maincenter function, adjust endocrine, take a tonic or nourishing food to build up one's health vigour, Physiological function is improved, immunological regulation, antifatigue, anti-aging extend the time, enhance gonad function, resist norepinephrine, expand Cavernous body, by more than too many levels work, come reach it is more effective, more comprehensively, more scientific improvement male's sexual Purpose.

The invention of open (bulletin) number CN101628073A《It is a kind of for improve male's sexual, antifatigue preparation and Its preparation method》It discloses a kind of for improving male's sexual, antifatigue preparation and preparation method thereof.Wherein preparation is by black Beans, semen allii tuberosi, Chinese yam, Poria cocos, matrimony vine, Semen Cuscutae, arginine and zinc gluconate are with 140-210: 120-180: 115-175: 35-55: 30-60: 25-40: 20-35: 0.5-1.5 weight ratio is made.Provided by the present invention for improving male's sexual, resisting Preparation of fatigue and preparation method thereof is to extract the active ingredient in medicinal plants using the method for boiling extraction, and keeps it Then activity adds in basic material as replenishing essence production of sperm of arginine, zinc gluconate, so can not only make in drug effectively into The dissolution rate divided greatly improves, and contributes to the absorption of drug, so as to improve its bioavilability.

The invention of publication number CN104248736A《A kind of Chinese drugs agentia of dispelling effects of alcohol and nourishing liver》Disclose a kind of dispelling effects of alcohol and nourishing liver Chinese drugs agentia.By following components by weight：15-25 parts of rhizoma imperatae, 15-25 parts of galangal, 20-30 parts of mulberry, 15-25 parts of cloves, 10-20 parts of Radix Angelicae Sinensis, 10-20 parts of grass-leaved sweetflag, 5-15 parts of plantain seed, 5-15 parts of rhizome of chuanxiong, Chu is 5-15 parts sub- in fact, will Said components add in 300-500 parts of water in, boil 20 minutes, decoction.Medicament provided by the present invention, scientific formula are used Amount is reasonable, not only can effectively relieve the effect of alcohol, more can blood-nourishing tonifying liver, protect liver from alcohol damaged, conserve liver.

Above-mentioned patent is disclosed to improve male's sexual and prostate problems or dispelling effects of alcohol and nourishing liver using natural plant composition Food and drug, preparation method is simply mixed in a certain ratio after simple processing is extracted, and utilizes probiotics to natural Plant carries out fermentation and prepares to improve male's sub-health state and take into account the functional food of dispelling effects of alcohol and nourishing liver to have not been reported.

The content of the invention

It is contemplated that in view of the shortcomings of the prior art, providing a kind of improvement male's sub-health state takes into account dispelling effects of alcohol and nourishing liver effect Functional beverage and food, and the preparation method of the drink and food is provided, especially for having prostate problems and male Sexual function problem and the crowd often to drink beyond one's capacity, and provide Research Thinking for the production of Related product.

The present invention passes through the productions such as probiotics fermention turmeric and black pepper, sealwort, rape pollen, black soya bean, raspberry, matrimony vine Functional drink and food can not only retain its active ingredient, also with health role to body.Turmeric belongs to perennial Herbaceous plant wherein the curcumin contained is a kind of phenol sulfonate, has anti-inflammatory, anti-oxidant, chemoprevention of cancer, protection liver The effects that.The study found that curcumin can inhibit the growth of prostate gland cancer cell and induce the apoptosis of prostate gland cancer cell, to urgency Property alcoholic liver injury has protective effect.There is good development prospect in terms of the treatment of prostate cancer and anti-liver injury.But Traditional method for extracting curcumin difficulty is big, content is few, and is difficult to the utilization that is absorbed by the body.The present invention is by turmeric and black pepper, Huang The cooperations such as essence, and probiotics fermention method is used, the content of curcumin and its derivative is improved, and curcumin can be promoted in human body Interior absorption, this functional food of gain is improve in terms of male's sub-health state and dispelling effects of alcohol and nourishing liver the effect of.

It is an object of the invention to mix bacterium to turmeric, sealwort using probiotics, further include if necessary rape pollen, black soya bean, Raspberry, matrimony vine, black pepper are fermented, and obtain a kind of probiotics functional food.

To reach above-mentioned purpose, technical scheme is as follows：

A kind of probiotics fermention functional food for improving male's inferior health, is by turmeric, sealwort, further includes if necessary auxiliary Material, ferments in proportion；Using lactobacillus plantarum, animal bifidobacteria, Lactobacillus paracasei, bifidobacterium adolescentis, condensation gemma Bacillus and a variety of bacterial strains of bacillus subtilis are prepared after carrying out mixed bacterium solution state or solid state fermentation；

The auxiliary material is preferably at least one of black pepper, rape pollen, black soya bean, raspberry, matrimony vine；

The present invention provides a kind of prebiotic strains liquid fermentation drinks for improving male's inferior health, fermented and cultured based raw material group Into as follows：The curcuma powder of 2-8% (mass volume ratio), the sealwort powder of 2-8% are added in water, while can also be added in not more than It is 5% black pepper, not more than 3% rape pollen, not more than 3% black bean powder, not more than 3% raspberry powder, few One or more in 3% wolfberry fruit powder, stir evenly；

Preferably, the prebiotic strains liquid fermentation drinks for improving male's inferior health, fermented and cultured based raw material composition Further include the raspberry of the black pepper of 1-5% and/or the black bean powder and/or 1-3% of the rape pollen of 1-3% and/or 1-3% The wolfberry fruit powder of powder and/or 1-3%.

The present invention provides a kind of preparation method for the prebiotic strains liquid fermentation drinks for improving male's inferior health, specific to walk It is rapid as follows：

(1) by turmeric, black pepper, sealwort, black soya bean, raspberry, matrimony vine cleaning be placed on 60 DEG C of oven for drying 3-4 it is small when, Then crush, be milled 100 mesh sieves respectively, obtains curcuma powder, black pepper, sealwort powder, black bean powder, raspberry powder, wolfberry fruit powder.

(2) activation medium is prepared：12g black bean powders are added in per 100ml water, obtain activation medium；To activation medium It sterilizes, condition is 121 DEG C, 20min；

(3) fermentation medium is prepared：The fermentation medium is：Curcuma powder, sealwort powder are added in water, it can also be simultaneously Add the one or more in black pepper, rape pollen, black bean powder, raspberry powder, wolfberry fruit powder；

It is described preparation fermentation medium be specially：2-8g curcuma powders, 2-8g sealwort powder are added in per 100ml water, in addition may be used The black pepper and/or 1-3g rape pollens and/or the raspberry powder and/or 1- of 1-3g black bean powders and/or 1-3g of addition 1-5g The wolfberry fruit powder of 3g obtains liquid state fermentation culture medium；

(4) actication of culture：Lactobacillus plantarum, animal bifidobacteria, Lactobacillus paracasei, bifidobacterium adolescentis and condensation bud Spore bacillus, bacillus subtilis glycerol tube respectively by 1% (volume ratio of v/v activation mediums) be inoculated in activation medium into Row activation, pH is naturally, controlled at 37 DEG C；After activating for 24 hours respectively, by 10% (v/v) connect bacterium amount again transfer activation training It supports base and carries out re-activation, reactivation obtains secondary seed solution afterwards for 24 hours；

(5) probiotics fermention：It is 10 by the inoculum concentration of animal bifidobacteria⁶-10⁷CFU/g, the inoculum concentration of lactobacillus plantarum For 10⁶-10⁷CFU/g, the inoculum concentration of Lactobacillus paracasei is 10⁵-10⁶CFU/g, the inoculum concentration of bifidobacterium adolescentis is 10⁶- 10⁷CFU/g, the inoculum concentration of bacillus coagulans is 10⁵-10⁶CFU/g, the inoculum concentration of bacillus subtilis is 10⁵-10⁶CFU/g It is inoculated in fermentation medium, mixed-strains liquid fermentation 24-48h, temperature is 37 DEG C；

(6) after fermentation, zymotic fluid improves male to obtain the final product through emulsifying plus after stabilizer, tune acid, blending, homogeneous, sterilization The prebiotic strains liquid fermentation beverage of inferior health；

The turmeric, black pepper, sealwort, rape pollen, black soya bean, raspberry, matrimony vine are commercially available；

Preferably, the composition of liquid state fermentation culture medium is：8g curcuma powders, 8g sealwort powder, the black Hus of 4g are added in per 100ml water Green pepper powder, 3g rape pollens, 2g black bean powders, 1g raspberry powders, 1g wolfberry fruit powders；

Preferably, the mixed-strains liquid fermentation time is for 24 hours；

Preferably, the lactobacillus plantarum be lactobacillus plantarum (Lactobacillus plantarum) TK9, the bacterial strain China Committee for Culture Collection of Microorganisms's common micro-organisms center, address are preserved on December 17th, 2015：Beijing No. 3 Institute of Microorganism, Academia Sinica of institute of city Chaoyang District North Star West Road 1, postcode 100101, deposit number CGMCC No.11891；This lactobacillus plantarum is with preferable anti-hydrochloric acid in gastric juice, the ability of anti-cholate, the 2h survival rates 88.03% in pH 3, 0.3% cholate 4h survival rates 81.06%, and there is extensive antifungal activity, additional preservative need not be added, it can also Extend the drink and food preservation time.It is wider than by Wang Hai and is collected in natural fermented acid in Chinese Residents In Bao Tou City man in July, 2009 Congee；

Preferably, the animal bifidobacteria is animal bifidobacteria (Bifidobacterium animalis) 937, should Bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, address on December 17th, 2015： Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica, postcode 100101, deposit number CGMCC No.11892；It is wider than by Wang Hai and is collected in Tianjin City, China in May, 2007；

Preferably, the Lactobacillus paracasei is Lactobacillus paracasei (Lactobacillus paracasei) TK1501, The bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, ground on October 21st, 2016 Location：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica, postcode 100101, deposit number are CGMCC No.13130；In August, 2015 is wider than by Wang Hai and is collected in natural fermented acid congee in Chinese Residents In Bao Tou City man；

Preferably, the bifidobacterium adolescentis is bifidobacterium adolescentis (Bifidobacterium adolescentis) TK99, the bacterial strain are preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on July 14th, 2016, Address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica, postcode 100101, deposit number For CGMCC No.12784；It is wider than by Wang Hai and is collected in Tianjin City, China in January, 2015；

Preferably, the bacillus coagulans be bacillus coagulans (Bacillus coagulans) TQ33, the bacterial strain China Committee for Culture Collection of Microorganisms's common micro-organisms center was preserved in 9th in September in 2011, address is：Beijing The institute 3 of city Chaoyang District North Star West Road 1, Institute of Microorganism, Academia Sinica, postcode 100101, deposit number are：CGMCC No.5233；It is wider than by Wang Hai and is collected in the skimmed milk power of Tianjin City, China in May, 2002；

Preferably, the bacillus subtilis is bacillus subtilis (Bacillus subtilis) TK-1, and the bacterial strain is China Committee for Culture Collection of Microorganisms's common micro-organisms center is preserved on April 2nd, 2011, address is：Beijing The institute 3 of Chaoyang District North Star West Road 1, Institute of Microorganism, Academia Sinica, postcode 100101, deposit number CGMCC No.4731；It is wider than by Wang Hai and is collected in inner mongolia Huhehaote in October, 2008.

Present invention simultaneously provides a kind of preparation method for the probiotics solid state fermentation food for improving male's inferior health, including such as Lower step：Using turmeric, black pepper, sealwort, rape pollen, black soya bean, raspberry, matrimony vine as fermentation substrate, using plant breast bar Bacterium, animal bifidobacteria, Lactobacillus paracasei, bifidobacterium adolescentis, bacillus coagulans and bacillus subtilis mix through solid-state It is prepared after bacterium fermentation；

A kind of preparation method for the probiotics solid state fermentation functional food for improving male's inferior health, is as follows：

(1) curcuma powder, sealwort powder and black pepper, rape pollen, black bean powder, raspberry powder, wolfberry fruit powder are chosen wherein One or several kinds are mixed by certain weight ratio, obtain the mixture of turmeric, sealwort and auxiliary material：Water=1：Solid-state is made in 0.8-2 Fermentation medium；The mixture of the turmeric, sealwort and auxiliary material：The ratio of water is weight ratio；

(2) sterilize to solid-state fermentation culture medium, condition is 121 DEG C, 20min；

(3) actication of culture：Mode is the same as above-mentioned liquid state fermentation；

(4) secondary seed solution is accessed in solid-state fermentation culture medium so that in every gram of solid medium, animal bifidobacteria Inoculum concentration be 10⁶-10⁷CFU/g, the inoculum concentration of lactobacillus plantarum is 10⁶-10⁷CFU/g, the inoculum concentration of Lactobacillus paracasei are 10⁵-10⁶CFU/g, the inoculum concentration of bifidobacterium adolescentis is 10⁶-10⁷CFU/g, the inoculum concentration of bacillus coagulans is 10⁵- 10⁶CFU/g, the inoculum concentration of bacillus subtilis is 10⁵-10⁶CFU/g；

(5) mixed culture solid state fermentation 24-48h, 37 DEG C；

After fermentation, after being positioned over -80 DEG C of pre-freeze 12h, it is put into vacuum freeze drier and freezes 12h, turmeric is made Probiotics solid state fermentation food；

Preferably, the mixture of the turmeric, sealwort and auxiliary material：Water=1：1.2；

Preferably, in the solid state fermentation, vacuum freeze drying condition carries out vacuum freeze drying after being -80 DEG C of pre-freeze 6h 12h, until water content is not more than 5%；

Preferably, the lactobacillus plantarum is lactobacillus plantarum (Lactobacillus plantarum) TK9, and preservation is compiled Number be CGMCC No.11891；

Preferably, the animal bifidobacteria is animal bifidobacteria (Bifidobacterium animalis) 937, is protected It is CGMCC No.11892 to hide number；

Preferably, the Lactobacillus paracasei is Lactobacillus paracasei (Lactobacillus paracasei) TK1501, Deposit number is CGMCC NO.13130；

Preferably, the bifidobacterium adolescentis is bifidobacterium adolescentis (Bifidobacteriumadolescentis) TK99, deposit number are CGMCC No.12784；

Preferably, the bacillus coagulans is bacillus coagulans (Bacillus coagulans) TQ33, and preservation is compiled Number it is：CGMCC No.5233；

Preferably, the bacillus subtilis be bacillus subtilis (Bacillus subtilis) TK-1, deposit number For CGMCC No.4731.

Advantageous effect

Turmeric belongs to herbaceous plant for many years, wherein the curcumin contained is a kind of phenol sulfonate, curcumin have it is anti-oxidant, Many-sided pharmacology such as free radical and protection cardiovascular system and protection liver is removed in anti-inflammatory, antitumor, antimicrobial, reducing blood lipid Effect.The study found that curcumin can inhibit the growth of prostate gland cancer cell and induce the apoptosis of prostate gland cancer cell, to acute Alcoholic liver injury has protective effect.There is good development prospect in terms of the treatment of prostate cancer and anti-liver injury.

The entire plant of black pepper all has medicinal and edible value, is usually used in treating constipation, chronic malaria, gonorrhoea, abdomen The diseases such as rush down, black pepper also has anti-inflammatory, anticancer, alleviates depressed, hypoglycemic, reducing blood lipid, weight-reducing, treats the work(such as hepatic injury in addition Can, can also stimulating intestine and stomach, promote intestines peristalsis.Pipering is one of major physiological active ingredient in black pepper, belongs to cassia bark Amide alkaloid has a variety of pharmacological activities such as anticonvulsion, anticancer, anti-oxidant, anti-inflammatory, promotion drug absorption.Pipering Effect as bioavilability reinforcing agent is increasingly taken seriously, can improve medicine from multipaths such as absorption, metabolism The bioavilability of object, therefore, addition black pepper can improve absorbing for curcumin in fermentation.

Sealwort is mild-natured, sweet in flavor, nontoxic, returns spleen, lung, kidney channel, has tonifying middle-Jiao and Qi, yin-nourishing invigorating the spleen, moistening lung kidney-nourishing, strengthening the bones and muscles Etc. functions, for deficiency of spleen-QI and stomach-QI, fatigue and asthenia, deficiency of stomach-Yin, dry deficiency of food, deficiency syndrome of the lung cough caused by dryness, overstrain cough hemoptysis, asthenia of essence and blood, waist The diseases such as knees soreness, poliosis, Heat Diabetes.Modern pharmacology research proves, sealwort have enhancing immune function, Adjust-blood lipid, The multiple pharmacological effects such as hypoglycemic, anti-aging, the clinical treatment available for diabetes, coronary heart disease, hyperlipidemia etc..

Rape pollen is full of nutrition, comprehensive, has plurality of health care functions, improving immunocompetence, antifatigue, reinforcement Power, brain tonic and intelligence development, anti-hypoxemia, adjusting endocrine, pre- anti-aging, skin maintenance etc., meanwhile, to cardiovascular and cerebrovascular disease, prostate Disease, liver disease, habitual constipation, anaemia, diabetes etc. have certain treatment and auxiliary treatment functio.Domestic and international clinical practice is equal Prove that pollen has good treatment chronic non-bacterial prostatitis, prostate pain, benign prostatic hyperplasis and prostate The effect of cancer.The effective component of pollen treatment prostatic disorders is mainly aliphatic acid, polyphenol, flavonoids, steroidal in its component And nitrogenous compound.

Black soya bean is sweet in flavor, mild-natured, and returns spleen, kidney channel have effects that benefiting shrewd head, nourishing the blood to expel wind, Li Shui, removing toxic substances.Modern times face Bed and pharmacological research show：Black soya bean has anti-oxidant, adjusting hormonal readiness, prevention of arterial vascular sclerosis, cosmetic hair-care, macrobiosis benefit The effects that intelligence, cancer-resisting.

Raspberry, sweet in flavor, slightly astringent taste, the flavones contained, organic acid, carbohydrate, vitamin, ellagic acid, cupreol and Trace elements etc. have the effect of anti-aging, adjust reproductive system, promote cellular immunity function, lose weight, have supporing yang contracting urine, kidney tonifying Controlling nocturnal emission with astringent drugs function, for treating the illnesss such as impotence, emission due to the kidney deficiency, frequent micturition and the enuresis.

Raspberry category rose family rubus Berry plant, dry fruit is medicine-food two-purpose fruit, rich in vitamin E, super oxygen The anti-agings substance such as object mutase, aminobutyric acid；Its nature and flavor is sweet, sour, and tepor returns liver and kidney channel, invigorates the kidney and stops nocturnal emission, supporing yang contracting urine Function can treat the illnesss such as emission due to the kidney deficiency, impotence, the enuresis and frequent micturition.In addition, raspberry also containing abundant cellulose and K, The various trace elements such as Zn, Fe, Cu, Mn, long-term consumption can effectively act as cardioprotection, prevent angiocardiopathy, anticancer with Antiobesity action has higher medicinal health Development volue.

The fruit of Chinese wolfberry is sweet in flavor, mild-natured, returns liver and kidney channel, there is tonifying liver, kidney-nourishing, the function of improving eyesight, available for treatment consumption consumptive loss, The illnesss such as soreness of waist and knee joint.The fruit of Chinese wolfberry is the Chinese medicine material of nutritional supplement and food, wherein the polysaccharides contained is matrimony vine The critical function factor, there is now many researchs show polysaccharides have promote immune, anti-aging, it is antitumor, remove free radical, The effects that antifatigue, radioresistance, liver protection, reproductive function protection and improvement.

Probiotics is a kind of by improving intestine microenvironment so as to the microorganism to host's generation beneficial effect.It can Inhibit growth of pathogenic bacteria by participating in intestinal microecology balance, avoid the drug resistance of long-time service antibiotic generation and double Infection, therefore, probiotic products receive the concern of people in recent years.

A kind of probiotics fermention functional food for improving male's inferior health of the present invention, had both saved the battalion of natural plants Value, and the nutrition health-care functions with probiotics fermention product are supported, it is unique in taste；The probiotics fermention can improve male The food that inferior health takes into account dispelling effects of alcohol and nourishing liver effect is prepared by specific process, by probiotics fermention, is made in natural plants Beneficial activity ingredient, it is easier to absorption of human body, enhancing improve male's sub-health state and the effect of dispelling effects of alcohol and nourishing liver.By prebiotic Bacterium fermentation improves the food and probiotics powder agent of male's inferior health and dispelling effects of alcohol and nourishing liver to prepare, and is ensureing that fermentation substrate is uniformly same When, it is easy to accomplish large-scale production.

Specific embodiment

With reference to embodiment and the experimental example technical solution that the present invention will be described in detail, specific embodiment described herein Be only used to explain the present invention, but the present invention is not limited to specific examples below and protection domain it is without being limited thereto.

The percentage sign " % " arrived involved in embodiment if not specified, refers to mass percent, and the percentage of solution refers to Grams containing solute in 100mL, the percentage between liquid refer to the volume ratio of the solution at 25 DEG C.

Embodiment 1

A kind of preparation method for the probiotics fermention drinks for improving male's inferior health

1st, turmeric in raw material, sealwort and ratio of adjuvant determine

(1) by turmeric, black pepper, sealwort, black soya bean, raspberry, matrimony vine cleaning be placed on 60 DEG C of oven for drying 3-4 it is small when, Then crush, be milled 100 mesh sieves respectively, obtains curcuma powder and black pepper, sealwort powder, black bean powder, raspberry powder, wolfberry fruit powder.

(2) activation medium is prepared：12g black bean powders are added in per 100ml water；It sterilizes to activation medium, condition For, 121 DEG C, 20min；

(3) fermentation medium is prepared：2-8g curcuma powders, 2-8g sealwort powder are added in per 100ml water, in addition can add 1-5g The wolfberry fruit powder of the raspberry powder and/or 1-3g of black pepper and/or 1-3g rape pollens and/or 1-3g black bean powders and/or 1-3g, Obtain liquid state fermentation culture medium；

(4) actication of culture, lactobacillus plantarum, animal bifidobacteria, Lactobacillus paracasei, bifidobacterium adolescentis, condensation bud The glycerol tube of spore bacillus and bacillus subtilis is inoculated in activation medium by 1% respectively and is activated, and pH is naturally, control temperature For 37 DEG C；After activating for 24 hours respectively, by 10% (v/v) connect bacterium amount transfer again activation medium carry out re-activation, reactivation Obtain secondary seed solution afterwards for 24 hours；

(5) probiotics fermention：It is 10 by the inoculum concentration of animal bifidobacteria⁶-10⁷CFU/g, the inoculum concentration of lactobacillus plantarum For 10⁶-10⁷CFU/g, the inoculum concentration of Lactobacillus paracasei is 10⁵-10⁶CFU/g, the inoculum concentration of bifidobacterium adolescentis is 10⁶- 10⁷CFU/g, the inoculum concentration of bacillus coagulans is 10⁵-10⁶CFU/g, the inoculum concentration of bacillus subtilis is 10⁵-10⁶CFU/g It is inoculated in fermentation medium, lactobacillus plantarum and animal bifidobacteria, Lactobacillus paracasei, bifidobacterium adolescentis, condensation bud Spore bacillus and bacillus subtilis connect bacterium amount volume ratio as 1：1：1：1：1：1, for 24 hours, temperature is 37 DEG C to mixed-strains liquid fermentation；Respectively Number of live bacteria of probiotics in zymotic fluid is detected, as a result such as table 1：

Strain label：No. 1 is lactobacillus plantarum TK9 single bacteriums, and No. 2 are 379 single bacterium of animal bifidobacteria, and No. 3 are secondary cheese Lactobacillus TK1501 single bacteriums, No. 4 are bifidobacterium adolescentis TK99 single bacteriums, and No. 5 are bacillus coagulans TQ33 single bacteriums, and No. 6 are withered Careless bacillus TK-1 single bacteriums.

The present invention has carried out a variety of natural material mixes liquid fermentations, and curcuma powder, Huang are added according to different proportion per 100ml Fine powder, black pepper, rape pollen, black bean powder, raspberry powder, wolfberry fruit powder carry out liquid state fermentation, and viable count the results are shown in Table 1.

Number of live bacteria of probiotics after the auxiliary material combination liquid state fermentation of 1 turmeric of table, sealwort and different proportion

	Combination one	Combination two	Combination three	Combination four	Combination five
						No. 1	1.49×10⁸	2.61×10⁸	4.32×10⁸	4.69×10⁸	7.42×10⁸
No. 2	1.09×10⁷	2.12×10⁸	3.06×10⁸	4.37×10⁸	8.08×10⁸
						No. 3	2.31×10⁷	8.94×10⁷	9.21×10⁷	9.91×10⁷	5.81×10⁸
No. 4	3.45×10⁶	1.82×10⁷	3.71×10⁷	5.72×10⁷	8.91×10⁷
						No. 5	1.61×10⁶	2.42×10⁷	4.19×10⁷	5.37×10⁷	7.91×10⁷
No. 6	1.12×10⁵	2.57×10⁶	4.49×10⁶	6.69×10⁶	8.61×10⁶
						Total viable count	5.65×10⁸	6.61×10⁸	7.21×10⁸	1.04×10⁹	4.35×10⁹

Each group is represented with label：Combination one：Turmeric：Sealwort：Black pepper:Rape pollen：Black soya bean：Raspberry：Matrimony vine= 6:4:3:3:2:2:3, combination two：Turmeric：Sealwort：Black pepper:Rape pollen：Black soya bean：Raspberry：Matrimony vine=2:4:3:2:2:1: 1, combination three：Turmeric：Sealwort：Black pepper:Rape pollen：Black soya bean：Raspberry：Matrimony vine=5:2:1:3:1:3:2, combination four：Ginger It is yellow：Sealwort：Black pepper:Rape pollen：Black soya bean：Raspberry：Matrimony vine=5:4:5:1:3:1:2, combination five:Turmeric：Sealwort：Black Hu Green pepper:Rape pollen：Black soya bean：Raspberry：Matrimony vine=8:8:4:3:2:1:1.

Curcuma powder is with after multiple auxiliary materials mixed fermentation, combining five as shown in Table 1：Turmeric：Black pepper：Sealwort:Rape pollen： Black soya bean：Raspberry：Matrimony vine=8:8:5:3:2:1:1, i.e., often 8g curcuma powders, 8g sealworts powder, 4g black peppers are added in 100ml water Viable count reaches highest when powder, 3g rape pollens, 2g black bean powders, 1g raspberry powders, 1g wolfberry fruit powders, and 4.35 × 10⁹CFU/mL。

2nd, probiotics inoculum concentration is definite

Other conditions are same as above, by addition 8g curcuma powders, 8g sealworts powder, 4g black peppers, 3g rape flowers in every 100ml water Powder, 2g black bean powders, 1g raspberry powders, 1g wolfberry fruit powders prepare fermentation medium, by lactobacillus plantarum TK9 by it is following connect bacterium amount 1 × 10⁶、5×10⁶、1×10⁷、5×10⁷CFU/mL is inoculated in fermentation medium respectively, the ratio of other strains and lactobacillus plantarum Under such as：Lactobacillus plantarum TK9, animal bifidobacteria 937, Lactobacillus paracasei TK1501, bifidobacterium adolescentis TK99, condensation Bacillus TQ33, the bacterium amount ratio that connects of bacillus subtilis TK-1 are 1：1：1：1：1：1,37 DEG C of fermentation, fermentation are surveyed after completing Determine the number of live bacteria of probiotics in zymotic fluid.

Viable count (CFU/mL) under 2 different vaccination amount of table

The results are shown in Table 2：Optimum inoculation amount lactobacillus plantarum TK9, animal bifidobacteria 937, Lactobacillus paracasei TK1501, bifidobacterium adolescentis TK99, bacillus coagulans TQ33, bacillus subtilis TK-1 are 1 × 10⁷CFU/mL.It surveys The total viable count obtained is 5.43 × 10⁹CFU/mL。

3rd, incubation time is definite

It is yellow that 8g curcuma powders, 8g are added under conditions of inoculum concentration has optimized, in the every 100ml water of liquid state fermentation culture medium selection Fine powder, 4g black peppers, 3g rape pollens, 2g black bean powders, 1g raspberry powders, 1g wolfberry fruit powders.

It is lactobacillus plantarum TK9, animal bifidobacteria 937, Lactobacillus paracasei TK1501, bifidobacterium adolescentis TK99, solidifying Knot bacillus TQ33, the glycerol tube of bacillus subtilis TK-1 are inoculated in black bean powder activation medium by 1% and are once lived Change, activate for 24 hours, then transfer by the inoculum concentration of 10% (v/v) in re-activation culture medium, re-activation is for 24 hours.In liquid Inoculated plant lactobacillus TK9, animal bifidobacteria 937, Lactobacillus paracasei TK1501, youth bifid bar in state fermentation medium Bacterium TK99, bacillus coagulans TQ33, bacillus subtilis TK-1 seed liquor mixed fungus fermentations.So that it is linked into turmeric liquid state fermentation It is lactobacillus plantarum TK9 in culture medium, animal bifidobacteria 937, Lactobacillus paracasei TK1501, bifidobacterium adolescentis TK99, solidifying Bacillus TQ33, bacillus subtilis TK-1 are tied, is 1 × 10⁷CFU/mL；PH is naturally, 37 DEG C of cultivation temperature, mixed fungus fermentation 12nd, 24,36,48h, is respectively detected the number of live bacteria of probiotics in zymotic fluid.

Influence of the 3 different fermentations time of table to number of live bacteria of probiotics after culture

	12h	24h	36h	48h
					No. 1	4.86×10⁸	5.19×10⁸	3.77×10⁸	3.94×10⁸
No. 2	5.09×10⁸	5.29×10⁸	2.21×10⁸	6.36×10⁸
					No. 3	9.81×10⁷	7.37×10⁷	6.71×10⁷	8.45×10⁷
No. 4	8.15×10⁷	6.47×10⁷	5.17×10⁷	7.49×10⁷
					No. 5	6.31×10⁷	7.69×10⁷	2.57×10⁷	6.01×10⁷
No. 6	6.39×10⁵	8.51×10⁵	3.98×10⁵	7.41×10⁵
					Total viable count	7.35×10⁸	9.01×10⁹	5.31×10⁹	4.71×10⁹

As a result shown in table 3：The optimal incubation time of mixed-strains liquid fermentation is for 24 hours, viable count is 9.01 × 10⁹CFU/ml。

4th, a kind of preparation method for the liquid state fermentation beverage for improving male's inferior health

(1) will be dried after turmeric and auxiliary material cleaning, 100 mesh sieves that were milled respectively obtain curcuma powder and various auxiliary material powder；

(3) fermentation medium is prepared：8g curcuma powders, 8g sealworts powder, 4g black peppers, 3g rapes are added in per 100ml water Pollen, 2g black bean powders, 1g raspberry powders, 1g wolfberry fruit powders；

(4) actication of culture, lactobacillus plantarum, animal bifidobacteria, Lactobacillus paracasei TK1501, bifidobacterium adolescentis TK99, bacillus coagulans TQ33, bacillus subtilis TK-1 glycerol tubes are inoculated in activation medium by 1% respectively and live Change, pH is naturally, controlled at 37 DEG C；After activating for 24 hours respectively, transfer again activation medium by the bacterium amount that connects of 10% (v/v) Re-activation is carried out, reactivation obtains secondary seed solution afterwards for 24 hours；

(5) probiotics fermention：By lactobacillus plantarum by connecing bacterium amount as 1 × 10⁷CFU/mL is inoculated in fermentation medium, together When be inoculated with animal bifidobacteria, Lactobacillus paracasei TK1501, bifidobacterium adolescentis TK99, bacillus coagulans TQ33, withered grass Bacillus TK-1, lactobacillus plantarum, animal bifidobacteria, Lactobacillus paracasei TK1501, bifidobacterium adolescentis TK99, condensation Bacillus TQ33, bacillus subtilis TK-1 connect bacterium amount volume ratio as 1：1：1：1：1：1, mixed-strains liquid fermentation for 24 hours, temperature For 37 DEG C of fermentations；

(6) zymotic fluid is after emulsification after fermentation plus stabilizer, stabilizer press the quality percent by volume with zymotic fluid Be added, additive amount be sodium carboxymethylcellulose 0.5%, xanthans 0.6%, emulsifier 0.8%；Add zymotic fluid mass body The different wheat ketose that product percentage is 8% carries out adjusting sour blending；At 65 DEG C, homogeneous once, is sterilized through 80 DEG C under conditions of 30MPa 15-20min obtains the probiotic fermentation beverage that can improve male's inferior health, which has dispelling effects of alcohol and nourishing liver effect concurrently.

5th, culture medium active ingredient changes before and after liquid-state fermentation technology-fermentation

It is lactobacillus plantarum TK9, animal bifidobacteria 937, Lactobacillus paracasei TK1501, bifidobacterium adolescentis TK99, solidifying Knot bacillus TQ33, bacillus subtilis TK-1 glycerol tubes are inoculated in black bean powder liquid culture medium by 1% respectively and carry out once Activation, activates for 24 hours, then transfers by the bacterium amount that connects of 10% (v/w) into re-activation culture medium (with an activation culture Base) in, re-activation for 24 hours, with turmeric：Black pepper：Sealwort:Rape pollen：Black soya bean：Raspberry：Matrimony vine=8:8:4:3:2:1:1 Liquid state fermentation culture medium, and inoculated plant lactobacillus TK9, animal bifidobacteria 937, Lactobacillus paracasei simultaneously is made TK1501, bifidobacterium adolescentis TK99, bacillus coagulans TQ33, bacillus subtilis TK-1 seed liquors, inoculum concentration difference are equal For 1 × 10⁷CFU/mL, at 37 DEG C, pH naturally, mixed fungus fermentation for 24 hours.Curcumin is measured using GB/T 223.75-2008 methods；Profit With Syrups by HPLC GABA content；Utilize the total phenols content of forint phenol reaction assay；It is surveyed using GB/T 23788-2009 methods Determine Flavone content；The content of amino nitrogen is measured using GB/T12143.2-89 methods；It is measured using ozone index method (ORAC) Polyphenoils content, the results are shown in Table 4：

Active ingredient changes before and after the functional beverage fermentation of the improvement male's inferior health of table 4

Each stage is represented with number：Content before 1- fermentations；Content after 2- mixed fungus fermentations；After 3- lactobacillus plantarums TK9 fermentations Content；Content after 4- animal bifidobacterias 937 ferment；Content after 5- Lactobacillus paracaseis TK1501 fermentations；6- youth bifid bars Content after bacterium TK99 fermentations；Content after 7- bacillus coagulanses TQ33 fermentations；Content after 8- bacillus subtilises TK-1 fermentations

From data above, curcuma powder, black pepper, sealwort powder, rape pollen, black bean powder, raspberry powder, wolfberry fruit powder After prebiotic strains liquid fermentation active ingredient be all improved.Especially through mixed fungus fermentation, content reaches curcumin after fermentation 8.81mg/g；GABA content increased after fermented, and liquid beverage GABA content is reached for 4.11mg/g after mixed fungus fermentation, It is above the single bacterium fermentation total phenols contents of and reaches 9.72mg GAE/g；After mixed fungus fermentation, Flavonoid substances content substantially increases Add, content reaches 1.91mg/g, and amino-acid nitrogen content increases before relatively fermenting.Mixed fungus fermentation sample is compared with unfermentable fermentation It liquid and is fermented using single bacterium, DPpH inhibiting rates have reached 47%.

Embodiment 2：The probiotics solid state fermentation food making method of male's inferior health can be improved

1st, raw material proportioning is definite

(1) dry, be milled after being respectively washed turmeric, black pepper, sealwort, rape pollen, black soya bean, raspberry, matrimony vine 100 mesh sieves.

(3) fermentation medium is prepared：It chooses curcuma powder, sealwort powder and black pepper, rape pollen, black bean powder, cover basin Sub- powder, wolfberry fruit powder one of which or several by certain weight ratio mixing, obtain the mixture of turmeric, sealwort and auxiliary material：Water= 1：Solid-state fermentation culture medium is made in 0.8-2；The mixture of the turmeric, sealwort and auxiliary material：The ratio of water is weight ratio；

(5) probiotics fermention：It is 10 by the inoculum concentration of animal bifidobacteria⁶-10⁷CFU/g, the inoculum concentration of lactobacillus plantarum For 10⁶-10⁷CFU/g, the inoculum concentration of Lactobacillus paracasei is 10⁵-10⁶CFU/g, the inoculum concentration of bifidobacterium adolescentis is 10⁶- 10⁷CFU/g, the inoculum concentration of bacillus coagulans is 10⁵-10⁶CFU/g, the inoculum concentration of bacillus subtilis is 10⁵-10⁶CFU/g It is inoculated in fermentation medium；Lactobacillus plantarum and animal bifidobacteria, Lactobacillus paracasei, bifidobacterium adolescentis, condensation bud Spore bacillus and bacillus subtilis connect bacterium amount volume ratio as 1：1：1：1：1：1, for 24 hours, temperature is 37 DEG C to mixed culture solid state fermentation；Respectively Number of live bacteria of probiotics in fermented sample is detected, as a result such as table 5：

Strain label：No. 1 is lactobacillus plantarum TK9 single bacteriums, and No. 2 are 379 single bacterium of animal bifidobacteria, and No. 3 are secondary cheese Lactobacillus TK1501 single bacteriums, No. 4 are bifidobacterium adolescentis TK99 single bacteriums, and No. 5 are bacillus coagulans TQ33 single bacteriums, and No. 6 are withered Careless bacillus TK-1 single bacteriums

Raw material chooses turmeric, sealwort, black pepper, rape pollen, black soya bean, raspberry, matrimony vine, raw material weight proportioning of soup processed label： Combination one：4:4:2:1:1:1:1, combination two：5:4:3:1:1:1:1, combination three：5:4:4:2:1:1:1, combination four：5:3:3: 2:2:1:1, combination five：5:5:4:2:1:1:1, combination six：3:4:1:2:2:1:1, combination seven：3:3:1:2:1:1:2, combination Eight：4:3:2:2:1:1:1, combination nine：4:3:1:2:2:1:1.

Number of live bacteria of probiotics after 5 curcuma powder of table, sealwort powder and auxiliary material different ratio solid state fermentation

Combination one

Combination two

Combination three

Combination four

Combination five

Combination six

Combination seven

Combination eight

Combination nine

No. 1

2.51×10⁸

3.51×10⁸

4.23×10⁸

6.65×10⁸

6.81×10⁸

7.49×10⁸

5.34×10⁸

4.82×10⁸

7.56×10⁸

No. 2

2.77×10⁷

4.38×10⁷

7.54×10⁷

8.76×10⁷

7.67×10⁸

2.64×10⁸

6.56×10⁸

4.67×10⁸

3.13×10⁸

No. 3

7.31×10⁷

8.36×10⁷

9.87×10⁷

4.76×10⁸

7.32×10⁸

5.34×10⁸

4.01×10⁸

3.17×10⁸

2.19×10⁸

No. 4

1.42×10⁷

8.27×10⁷

5.75×10⁸

7.88×10⁸

9.61×10⁸

6.36×10⁸

3.69×10⁸

4.16×10⁸

3.65×10⁸

No. 5

2.01×10⁷

4.68×10⁷

5.26×10⁷

7.38×10⁷

6.84×10⁸

5.25×10⁸

4.15×10⁸

3.19×10⁸

1.86×10⁸

No. 6

3.98×10⁵

8.01×10⁵

9.38×10⁵

3.26×10⁷

5.74×10⁷

7.91×10⁷

3.16×10⁷

4.27×10⁷

3.79×10⁷

Total viable count

1.87×10¹⁰

8.26×10⁹

7.26×10⁸

2.17×10⁹

3.71×10¹⁰

8.06×10⁹

9.13×10⁹

4.74×10⁸

2.41×10⁸

The optimal proportion obtained as shown in Table 5 is curcuma powder：Sealwort powder：Black pepper：Rape pollen：Black bean powder：Cover basin Sub- powder：Wolfberry fruit powder=5:5:4:2:1:1:1, total viable count is 3.71 × 10¹⁰CFU/mL。

2nd, strain inoculum concentration is definite

It is lactobacillus plantarum TK9, animal bifidobacteria 937, Lactobacillus paracasei TK1501, bifidobacterium adolescentis TK99, solidifying Knot bacillus TQ33, bacillus subtilis TK-1 glycerol tubes are inoculated in activation medium by 1% respectively and (add in per 100ml water Add 12g black bean powders, sterilising conditions are 121 DEG C, 20min；) once activated；It activates for 24 hours, then presses 10% (v/w) The bacterium amount that connects transfer in re-activation culture medium (with an activation medium), re-activation for 24 hours, by curcuma powder：Sealwort powder： Black pepper：Rape pollen：Black bean powder：Raspberry powder：Wolfberry fruit powder=5:5:4:2:1:1:1, material-water ratio 1：1.2 are made solid-state Fermentation medium, while inoculated plant lactobacillus TK9, animal bifidobacteria 937, Lactobacillus paracasei TK1501, youth bifid Bacillus TK99, bacillus coagulans TQ33, bacillus subtilis TK-1 seed liquors carry out mixed fungus fermentation, mixed culture solid state fermentation for 24 hours, 37 DEG C, pH is natural.Number of live bacteria of probiotics in fermentation wild Oryza species is detected.

Inoculum concentration：By lactobacillus plantarum TK9 (L.plantarum TK9), 937 (B.animalis of animal bifidobacteria 937), Lactobacillus paracasei TK1501 (Lactobacillus paracaseiTK1501), bifidobacterium adolescentis TK99 (Bifidobacterium adolescentisTK99), bacillus coagulans TQ33 (Bacillus coagulansTQ33), Bacillus subtilis TK-1 (Bacillus subtilisTK-1) while solid-state fermentation culture medium is accessed, inoculum concentration is：1× 10⁶CFU/mL；5×10⁶CFU/mL；1×10⁷CFU/mL；5×10⁷Tetra- groups of CFU/mL, the results are shown in Table 6：

Viable count (CFU/g) under 6 solid state fermentation different vaccination amount of table

The results are shown in Table 6：Lactobacillus plantarum TK9 (L.plantarum TK9), animal bifidobacteria 937 (B.animalis937), Lactobacillus paracasei TK1501 (Lactobacillus paracaseiTK1501), youth bifid bar Bacterium TK99 (Bifidobacterium adolescentisTK99), bacillus coagulans TQ33 (Bacillus CoagulansTQ33), the optimum inoculation amount of bacillus subtilis TK-1 (Bacillus subtilisTK-1) for 5 × 10⁷CFU/g, that is, measure gained improve total viable count in the probiotics fermention functional food of male's inferior health for 9.67 × 10¹⁰CFU/g.Wherein, lactobacillus plantarum viable count is 7.11 × 10⁹CFU/g, the viable count of animal bifidobacteria reaches 5.15 × 10⁸CFU/g, Lactobacillus paracasei viable count are 2.37 × 10⁸CFU/g, bifidobacterium adolescentis viable count are 5.69 × 10⁸CFU/ G, coagulated bacillus living number are 8.41 × 10⁷CFU/g, the viable count of bacillus subtilis is 3.62 × 10⁶CFU/g。

3rd, material-water ratio is definite

Solid-state fermentation culture medium：The ratio of raw material and water (mass volume ratio) is respectively 1：0.8,1：1,1：1.2,1：1.6 1：1.8,1：2.

It is lactobacillus plantarum TK9, animal bifidobacteria 937, Lactobacillus paracasei TK1501, bifidobacterium adolescentis TK99, solidifying Knot bacillus TQ33, bacillus subtilis TK-1 glycerol tubes are inoculated in black bean powder liquid culture medium by 1% respectively and carry out once Activation, activates for 24 hours, then transfers by the bacterium amount that connects of 10% (v/w) into re-activation culture medium (with an activation culture Base) in, re-activation for 24 hours, with curcuma powder：Sealwort powder：Black pepper：Rape pollen：Black bean powder：Raspberry powder：Wolfberry fruit powder= 5:5:4:2:1:1:1 is made solid-state fermentation culture medium, and inoculated plant lactobacillus TK9, animal bifidobacteria 937, pair are dry simultaneously Lactobacillus paracasei TK1501, bifidobacterium adolescentis TK99, bacillus coagulans TQ33, bacillus subtilis TK-1 seed liquors, inoculation Amount is 5 × 10 respectively⁷CFU/mL carries out mixed fungus fermentation.Condition of culture：It is closed for 24 hours, 37 DEG C, pH is natural.To being cultivated after fermentation Number of live bacteria of probiotics in base is detected, and the results are shown in Table 7：

The proportioning of 7 curcuma powder of table and water is 1：During 0.8-2, number of live bacteria of probiotics (CFU/g) after fermentation

The results are shown in Table 7：Optimal material-water ratio is 1：1.2, that is, it measures in gained turmeric probiotics fermention functional food Total viable count be 5.79 × 10⁹CFU/g.Wherein, lactobacillus plantarum viable count is 8.71 × 10⁸CFU/g, animal bifidobacteria Viable count reach 4.37 × 10⁸CFU/g, the viable count of Lactobacillus paracasei is 5.11 × 10⁸CFU/g, bifidobacterium adolescentis Viable count is 6.27 × 10⁸CFU/g, the viable count of bacillus coagulans is 3.15 × 10⁸CFU/g, the viable bacteria of bacillus subtilis Number is 7.29 × 10⁷CFU/g。

4th, incubation time is definite

It is lactobacillus plantarum TK9, animal bifidobacteria 937, Lactobacillus paracasei TK1501, bifidobacterium adolescentis TK99, solidifying Knot bacillus TQ33, bacillus subtilis TK-1 glycerol tubes are inoculated in black bean powder liquid culture medium by 1% respectively and carry out once Activation, activates for 24 hours, then transfers by the bacterium amount that connects of 10% (v/w) into re-activation culture medium (with an activation culture Base) in, re-activation for 24 hours, the inoculated plant lactobacillus TK9, dynamic simultaneously in the optimized solid-state fermentation culture medium of material-water ratio Object Bifidobacterium 937, Lactobacillus paracasei TK1501, bifidobacterium adolescentis TK99, bacillus coagulans TQ33, bacillus subtilis Bacterium TK-1 seed liquors, inoculum concentration are 5 × 10 respectively⁷CFU/g carries out mixed fungus fermentation.Mix respectively bacterium sealed fermenting 12,24,36, 48h, 37 DEG C, pH is natural.Number of live bacteria of probiotics in fermentation wild Oryza species is detected.

Probiotics viable bacteria after the culture of 8 different fermentations time of table

	12h	24h	36h	48h
					No. 1	3.26×10⁸	4.89×10⁸	6.54×10⁸	3.75×10⁸
No. 2	4.65×10⁸	2.61×10⁸	5.16×10⁸	2.67×10⁸
					No. 3	5.61×10⁷	8.92×10⁷	4.66×10⁸	2.15×10⁸
No. 4	8.45×10⁷	9.51×10⁷	2.71×10⁸	1.07×10⁸
					No. 5	6.73×10⁶	1.07×10⁷	2.59×10⁷	8.65×10⁶
No. 6	2.72×10⁵	3.26×10⁵	4.75×10⁵	4.19×10⁵
					Total viable count	5.56×10⁹	5.11×10¹⁰	8.01×10¹⁰	4.78×10⁹

The results are shown in Table 8：Optimal incubation time is mixed fungus fermentation 36h again after inoculation, total viable count for 8.01 × 10¹⁰CFU/g。

5th, culture medium active ingredient changes before and after solid-state fermentation process-fermentation

It is lactobacillus plantarum TK9, animal bifidobacteria 937, Lactobacillus paracasei TK1501, bifidobacterium adolescentis TK99, solidifying Knot bacillus TQ33, bacillus subtilis TK-1 glycerol tubes are inoculated in black bean powder liquid culture medium by 1% respectively and carry out once Activation, activates for 24 hours, then transfers by the bacterium amount that connects of 10% (v/w) into re-activation culture medium (with an activation culture Base) in, re-activation for 24 hours, with curcuma powder：Sealwort powder：Black pepper：Rape pollen：Black bean powder：Raspberry powder：Wolfberry fruit powder= 5:5:4:2:1:1:1, material-water ratio 1：1.2 are made solid-state fermentation culture medium, and inoculated plant lactobacillus TK9, animal bifid simultaneously Bacillus 937, Lactobacillus paracasei TK1501, bifidobacterium adolescentis TK99, bacillus coagulans TQ33, bacillus subtilis TK-1 Seed liquor, inoculum concentration are 5 × 10 respectively⁷CFU/mL, at 37 DEG C, pH is naturally, mixed fungus fermentation 36h.Utilize GB/T223.75- 2008 methods measure curcumin；Utilize Syrups by HPLC GABA content；Utilize the total phenols content of forint phenol reaction assay；It utilizes GB/T 23788-2009 methods measure Flavone content；The content of amino nitrogen is measured using GB/T12143.2-89 methods；Utilize work Oxygen index method (ORAC) measures polyphenoils content, the results are shown in Table 9：

Active ingredient changes before and after the solid-state food fermentation of the improvement male's inferior health of table 9

From data above, curcuma powder, black pepper, sealwort powder, rape pollen, black bean powder, raspberry powder, wolfberry fruit powder After probiotics mixed culture solid state fermentation active ingredient be all improved.Content reaches 9.97mg/g to curcumin after fermentation； GABA content increased after fermented, and GABA content is reached for 5.25mg/g after mixed fungus fermentation, and it is total to be above single bacterium fermentation Phenols content reaches 10.61mg GAE/g；After mixed fungus fermentation, Flavonoid substances content substantially increases, and content reaches 2.35mg/ G, amino-acid nitrogen content increase before relatively fermenting.Mixed fungus fermentation sample is fermented compared with non-fermentation medium and using single bacterium, Its DPpH inhibiting rate has reached 57%.

3 prebiotic strains liquid fermentation beverage of embodiment and solid state fermentation beverage animal kidney tonifying experiment

Zoopery is carried out to prebiotic strains liquid fermentation beverage and solid beverage, and gavages physiological saline with control group and carries out Comparison.Experimental method is as follows：

Experimental animal：Wistar male white rats 80 of 1 month from breast, weight 150-180g.

Tested material dosage：5g/ only, continuously takes 20 days.

Experimental method：90 rats are randomly divided into prebiotic strains liquid fermentation beverage and tested group of solid beverage and control Group, every group 30, by the rat of prebiotic tested group one of strains liquid fermentation beverage feeding, by tested group of probiotics solid beverage feeding Two rat, control group：By wherein 30 similary rearing conditions of rat, normal saline is gavaged.Test method is with reference to China The telotism test method of Xiyuan hospital of the academy of traditional Chinese medicine carries out, and records eclipse period of penile erection, and carries out statistics between group Analysis.Result of the test is shown in Table 10：

10 prebiotic strains liquid fermentation beverage of table and solid state fermentation beverage are to animal kidney tonifying experimental result

The experimental results showed that a certain amount of prebiotic strains liquid fermentation beverage provided by the present invention and solid beverage can improve Castrated rats penis is to the excitability of outside stimulus, and under local micro electro photoluminescence effect, rat eclipse period of penile erection is apparent Shorten, compared with the control group, there is apparent strengthening yang and invigorating kidney.

4 prebiotic strains liquid fermentation beverage of embodiment and the research of the high male sexual function effect of solid state fermentation beverage lifting

With male mice, weight 25-28g is random to be grouped, experimental group every group 10, control group 10.Experimental group fills respectively The liquid and solid state fermentation beverage 5g/ day of the preparation of embodiment 1,2 are taken, control group gavages normal saline, one time a day, persistently 24 days.From the 4th day, each group animal daily when 25 DEG C of -30 DEG C of deep water middle reaches leaching 3.5 is small as stress, continue three weeks.It is taking With one week and take the 25th and do sexual function experiment.

Female adult mouse, weight 25-30g.To increase the Oestrus rate of animal, intramuscular injection female hormone diethylstilbestrol After 0.4mg/kg/ days, 2 days, vaginal smear microscopy using the seedless keratinocyte in surface layer as main person, shows, in estrus, to record With.This heat female mice, 5 cage packing.In 6 points of early morning, the male mice of an experiment is added per cage, is observed in 5 minutes The number of copulations and mating rate of generation.Specific experiment the results are shown in Table 11:

The influence of 11 prebiotic strains liquid fermentation beverage of table and solid state fermentation beverage to mouse sexual function

It gavages as shown in Table 11 after a week, experimental group mates frequency apparently higher than control group mating frequency, there is conspicuousness meaning Justice, experimental group mating rate are also higher than control group mating rate.It gavages the 25th, the sexual function of control animals is decreased obviously, and real The mating rate of group animal is tested without change, only mating frequency number is reduced, and experimental group mating frequency mates apparently higher than control group Frequency, there is significant, and experimental group mating rate is also higher than control group mating rate, there is significant.

From table 10,11 data of table, prebiotic strains liquid fermentation beverage and solid state fermentation beverage energy provided by the present invention Enough shorten the eclipse period of penile erection of rat, improve mouse sexual function, show that this product can effectively adjust animal bodies function, Tonifying kidney and strengthening yang improves male's inferior health.

5 prebiotic strains liquid fermentation beverage of embodiment and the experiment of solid state fermentation beverage animal relieving alcoholism and protecting liver

Experimental method：30 rats are randomly divided into three groups, every group 10, gavage 56 ° of white wine 3g daily, experimental group 1 is same When gavage prebiotic strains liquid fermentation beverage 5g, experimental group 2 gavages probiotics solid beverage 5g simultaneously, and control group gavages 5g lifes simultaneously Brine is managed, once a day, after gavaging six weeks, fasting 12h, broken end takes blood, and after standing 2h, centrifugation separates serum, -80 DEG C of storages It is spare.Serum aspartate amino transferase, alanine aminotransferase detection are carried out by the specification operation of kit.Tool Body the results are shown in Table 12：

Table 12 each group mice serum AST, ALT compare (X ± S)

Group	AST(U/L)	ALT(U/L)
			Experimental group 1	71.67±7.22	50.67±1.58
Experimental group 2	72.56±6.35	51.42±1.77
			Control group	83.32±6.18	58.25±2.59

Through animal mouse experiment, probiotics liquid beverage provided by the present invention can be reduced significantly with solid beverage Blood AST, ALT of mouse are horizontal, have and significantly relieve the effect of alcohol, protect liver cell, mitigate effect of the alcohol to hepatocellular injury.

Embodiment described above only expresses the several embodiments of the present invention, and description is more specific and detailed, but simultaneously Cannot the limitation to the scope of the claims therefore be interpreted as.It should be pointed out that for those of ordinary skill in the art, On the premise of not departing from this patent design, the respective embodiments described above can also make several deformations, combination and improve, these all belong to In the protection domain of this patent.Therefore, the protection domain of this patent should be determined by the appended claims.

Claims

1. a kind of preparation method for the probiotics fermention functional food for improving male's inferior health, step include：To include turmeric, Huang The raw material of essence is fermentation medium, using lactobacillus plantarum, animal bifidobacteria, Lactobacillus paracasei, bifidobacterium adolescentis, solidifying Knot bacillus, bacillus subtilis mixed fungus fermentation and obtain；It is characterized in that, the lactobacillus plantarum is lactobacillus plantarum (Lactobacillus plantarum) TK9, deposit number are CGMCC No.11891；The animal bifidobacteria is animal Bifidobacterium (Bifidobacterium animalis) 937, deposit number are CGMCC No.11892, the pair cheese breast bar Bacterium be Lactobacillus paracasei (Lactobacillus paracasei) TK1501, deposit number be CGMCC No.13130, the youth Bifidobacterium is bifidobacterium adolescentis (Bifidobacterium adolescentis) TK99, deposit number CGMCC No.12784, bacillus coagulans are bacillus coagulans (Bacillus coagulans) TQ33, deposit number CGMCC No.5233, the bacillus subtilis are bacillus subtilis (Bacillus subtilis) TK-1, deposit number CGMCC No.4731。

2. improving the preparation method of the probiotics fermention functional food of male's inferior health according to claim 1, feature exists In the raw material of the fermentation medium further includes at least one of black pepper, rape pollen, black soya bean, raspberry, matrimony vine.

3. improving the preparation method of the probiotics fermention functional food of male's inferior health according to claim 2, feature exists In including the following steps：

(1) by turmeric, black pepper, sealwort, black soya bean, raspberry, matrimony vine cleaning be placed on 60 DEG C of oven for drying 3-4 it is small when, then It crushes, be milled 100 mesh sieves respectively, obtains curcuma powder, black pepper, sealwort powder, black bean powder, raspberry powder, wolfberry fruit powder；

(2) activation medium is prepared：12g black bean powders are added in per 100ml water, obtain activation medium；Activation medium is carried out Sterilizing, condition is 121 DEG C, 20min；

(3) fermentation medium is prepared：The fermentation medium is：Curcuma powder, sealwort powder and black pepper, oil are added in water At least one of cauliflower powder, black soya bean, raspberry, matrimony vine；

(4) actication of culture：Lactobacillus plantarum, animal bifidobacteria, Lactobacillus paracasei, bifidobacterium adolescentis and condensation gemma bar Bacterium, the glycerol tube of bacillus subtilis are inoculated in activation medium by 1% (volume ratio of v/v activation mediums) respectively and live Change, pH is naturally, controlled at 37 DEG C；After activating for 24 hours respectively, transfer again activation medium by the bacterium amount that connects of 10% (v/v) Re-activation is carried out, reactivation obtains secondary seed solution afterwards for 24 hours；

(5) probiotics fermention：It is 10 by the inoculum concentration of animal bifidobacteria⁶-10⁷CFU/g, the inoculum concentration of lactobacillus plantarum are 10⁶-10⁷CFU/g, the inoculum concentration of Lactobacillus paracasei is 10⁵-10⁶CFU/g, the inoculum concentration of bifidobacterium adolescentis is 10⁶- 10⁷CFU/g, the inoculum concentration of bacillus coagulans is 10⁵-10⁶CFU/g, the inoculum concentration of bacillus subtilis is 10⁵-10⁶CFU/g It is inoculated in fermentation medium, mixed-strains liquid fermentation 24-48h, temperature is 37 DEG C.

4. improving the preparation method of the probiotics fermention functional food of male's inferior health according to claim 3, feature exists In fermentation medium composition is as follows：The curcuma powder of 2-8%, the sealwort powder of 2-8% are added in water, while add in not more than 5% Black pepper, not more than 3% rape pollen, not more than 3% black bean powder, not more than 3% raspberry powder, not more than One or more in 3% wolfberry fruit powder, stir evenly；

" % " is quality percent by volume.

5. improving the preparation method of the probiotics fermention functional food of male's inferior health according to claim 4, feature exists In fermentation medium composition is：In water add in 2-8% curcuma powder, the sealwort powder of 2-8%, the black pepper of 1-5% and/ Or the wolfberry fruit powder of the raspberry powder and/or 1-3% of the rape pollen of 1-3% and/or the black bean powder of 1-3% and/or 1-3%；

" % " is quality percent by volume.

6. improving the preparation method of the probiotics fermention functional food of male's inferior health according to claim 5, feature exists In the composition of the fermentation medium is：8g curcuma powders, 8g sealwort powder, 4g black peppers, 3g rapes are added in per 100ml water Pollen, 2g black bean powders, 1g raspberry powders, 1g wolfberry fruit powders.

7. improving the preparation method of the probiotics fermention functional food of male's inferior health according to claim 3, feature exists In the mixed-strains liquid fermentation time is for 24 hours.

8. improving the preparation method of the probiotics fermention functional food of male's inferior health according to claim 1, feature exists In including the following steps：

(1) curcuma powder, sealwort powder and wherein one in black pepper, rape pollen, black bean powder, raspberry powder, wolfberry fruit powder are chosen Kind or several mixing, obtain the mixture of turmeric, sealwort and auxiliary material：Water=1：Solid-state fermentation culture medium is made in 0.8-2；It is described The mixture of turmeric, sealwort and auxiliary material：The ratio of water is weight ratio；

(3) actication of culture：

Prepare activation medium：12g black bean powders are added in per 100ml water, obtain activation medium；It goes out to activation medium Bacterium, condition are 121 DEG C, 20min；

Actication of culture：Lactobacillus plantarum, animal bifidobacteria, Lactobacillus paracasei, bifidobacterium adolescentis and bacillus coagulans, The glycerol tube of bacillus subtilis is inoculated in activation medium by 1% (v/v) respectively and is activated, pH naturally, controlled at 37℃；After activating for 24 hours respectively, by 10% (v/v) connect bacterium amount transfer again activation medium carry out re-activation, reactivation Obtain secondary seed solution afterwards for 24 hours；

(4) secondary seed solution is accessed in solid-state fermentation culture medium so that in every gram of solid medium, animal bifidobacteria connects Kind amount is 10⁶-10⁷CFU/g, the inoculum concentration of lactobacillus plantarum is 10⁶-10⁷CFU/g, the inoculum concentration of Lactobacillus paracasei is 10⁵- 10⁶CFU/g, the inoculum concentration of bifidobacterium adolescentis is 10⁶-10⁷CFU/g, the inoculum concentration of bacillus coagulans is 10⁵-10⁶CFU/g, The inoculum concentration of bacillus subtilis is 10⁵-10⁶CFU/g；

(5) mixed culture solid state fermentation 24-48h, 37 DEG C；

After fermentation, after being positioned over -80 DEG C of pre-freeze 12h, it is put into vacuum freeze drier and freezes 12h, it is prebiotic that turmeric is made Bacterium solid state fermentation food.

9. improving the preparation method of the probiotics fermention functional food of male's inferior health according to claim 9, feature exists In the mass volume ratio of the solid-state fermentation culture medium raw material and water is 1：1.2；Each raw material weight ratio is：Curcuma powder：Sealwort Powder：Black pepper：Rape pollen：Black bean powder：Raspberry powder：Wolfberry fruit powder=5:5:4:2:1:1:1.

10. any preparation methods of the claim 1-9 probiotics fermention functional food obtained for improving male's inferior health.