CN106578346A - Technology of using froggrass to prepare dairy cow fermented feed through bioanalysis - Google Patents
Technology of using froggrass to prepare dairy cow fermented feed through bioanalysis Download PDFInfo
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- CN106578346A CN106578346A CN201611241712.8A CN201611241712A CN106578346A CN 106578346 A CN106578346 A CN 106578346A CN 201611241712 A CN201611241712 A CN 201611241712A CN 106578346 A CN106578346 A CN 106578346A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention discloses a dairy cow fermented feed. According to the dairy cow fermented feed, terrestrial alkaline plant resource froggrass is adopted as a raw material, microbial fermentation is used to produce the dairy cow fermented feed which has high content of protein and strong palatability, and is rich in probiotics of brewer's yeast, bacillus subtilis and the like, high-valued utilization of saline and alkaline terrestrial plant resource froggrass is achieved, and the disadvantage that dairy cow protein feed is scanty is solved. According to the dairy cow fermented feed, the froggrass is used as the raw material, seaweed residues, lucerne meal, straw, Chinese herbal medicine and the like are supplemented, and the dairy cow fermented feed is prepared through a technology of two-stage fermentation of a biological leavening agent; the dairy cow fermented feed is rich in probiotics of saccharomycetes, aspergillus niger, bacillus subtilis, brewer's yeast, lactic acid bacteria and the like, the content of the probiotics reaches up to 2-5*10<8>cfu/g, after the dairy cow fermented feed is used in cow breeding, immunity of the organism of a dairy cow can be remarkably improved, growth of the dairy cow can be promoted, milk yield can be increased, milk quality can be improved, and the dairy cow fermented feed has very high application and popularization value.
Description
Technical field
The present invention relates to a kind of bioanalysises prepare the technique of milch cow fermented feed using Salicornia Bigelovii Torr., belong to bioengineering field.
Background technology
Protein raw material is the basis of feed industrial development, and the in short supply and price of China's protein raw material is surging to be had become
One of bottleneck of restriction animal husbandry development.Now with the improvement of people's living standards and the attention to quality of life, to meat,
Egg, milk, aquatic products demand it is increasing, grain and Feed Manufacturing face a severe test, and feed protein resource will be increasingly deficient,
Therefore utilization rate of the grain to the transformation efficiency and feedstuff of livestock productss is improved, feed resource is expanded, exploitation unconventional water resources is
It is extremely urgent.And the Critical policies for alleviating the problems are aiming at existing protein sources, its utilization rate is improved, excavate feedstuff as much as possible
Using nutritional labeling, fundamentally alleviate feedstuff situation in short supply.China milk industry is quick through more than ten years
Development, China's cow population alreadys exceed 15,000,000 within 2013, and forage protein scarcity of raw material becomes restriction China milch cow
The bottleneck of aquaculture development, developing new forage protein raw material becomes the matter of utmost importance of China's milk cow production sustainable development.
Salicornia Bigelovii Torr. belongs to jasmine section plant also known as Salicornia Bigelovii Torr., extra large Cornu Cervi Pantotrichum, marine borer grass, rich and honour dish etc., and Salicornia Bigelovii Torr. category is given birth in salt
In plant classification, belong to stem carnification euhalophyte, be more common in marshland, by salt lake and and seabeach.Salicornia Bigelovii Torr. its be dried plant
Strain cellulose 5~20%, protein 9.0%, rich in 8 kinds of aminoacid, trace element and vitamin needed by human, wherein recklessly
Radix Raphani cellulose content exceedes 40 times of common vegetable, also containing natural plant health salt and natural plant alkaloid (micro- angle saponin), tool
There is the effects such as reduction cholesterol, blood pressure lowering.Salicornia Bigelovii Torr. stock number is big, and the plantation of Salicornia Bigelovii Torr. has weight to improving China's saline-alkali environment
Using value is wanted, but the comprehensive development and utilization degree of current Salicornia Bigelovii Torr. is relatively low, governs Salicornia Bigelovii Torr. secondary industry economic benefit,
The plantation that directly affects Salicornia Bigelovii Torr. is promoted, therefore, if Salicornia Bigelovii Torr. is carried out high-valued comprehensive development and utilization, will to environment and
Economy makes significant contribution.
The content of the invention
Deficient for China's milk cattle cultivating albumen feedstuff, the present situation based on ensilage, the present invention provide one kind with land
Raw alkaline soil plant resource Salicornia Bigelovii Torr. is raw material, using fermentable production protein content it is high, palatability is strong, rich in wine brewing ferment
The milch cow fermented feed of the probiotic bacterias such as mother, bacillus subtilises, contributes to realizing the high level of saline and alkaline terrestrial plant resource Salicornia Bigelovii Torr.
The drawbacks of changing utilization, solution cow protein feed scarcity.
Above-mentioned purpose of the present invention is achieved by the following technical programs:
The present invention provides the technique that a kind of bioanalysises prepare milch cow fermented feed using Salicornia Bigelovii Torr., it is characterised in that milch cow sends out
The raw material of ferment feedstuff is consisted of:60~80 parts of Salicornia Bigelovii Torr. yeast powder, 8~12 parts of Sargassum slag, 8~12 parts of alfalfa meal, powder of straw 10
Part, 1 part of compound Chinese herb, 2~3 parts of bio-fermentation agent, 1~2 part of cellulase.
Above-mentioned Salicornia Bigelovii Torr. yeast powder is prepared by following steps:
(1) crush immersion:Deimpurity Salicornia Bigelovii Torr. cane will be gone to crush, cross 100 mesh sieves, adjustment Salicornia Bigelovii Torr. cane powder will be aqueous
Amount 40~50%;
(2) digest:Add the cellulase of Salicornia Bigelovii Torr. quality 1% and 1% non-starch polysaccharide enzyme, 40~60 DEG C of enzymes of temperature control
Solution 24h;
(3) one-step fermentation:The salt tolerant Alkaliphilic bacillus of inoculation Salicornia Bigelovii Torr. mass ratio 2~3%, under the conditions of 28~35 DEG C
3~5d of fermentation, stirring in 12 hours is once;
(4) two-step fermentation:One-step fermentation is inoculated with 1~2% aspergillus niger, 1~2% saccharomyces cerevisiae after terminating, naturally rotten
Ripe 30~50d, obtains final product Salicornia Bigelovii Torr. yeast powder after fermentation ends.
Above-mentioned Sargassum slag can be Thallus Laminariae (Thallus Eckloniae), sargassum thunbergii, Alga Sgrgassi Enervess, Thallus Sargassi Kjellmaniani, the algae-residue of one or more in Entermorpha.
Above-mentioned powder of straw can be Semen Tritici aestivi, Semen Maydiss, one or more in rice straw of mixture.
The raw material of above-mentioned compound Chinese herb is consisted of:10 parts of Herba Leonuri, 10 parts of Herb Gynostemmae Pentaphylli, 5 parts of Massa Medicata Fermentata, 5 parts of Herba Houttuyniae, Pueraria lobota
3 parts of root, 2 parts of Radix Glycyrrhizae.
Above-mentioned bio-fermentation agent is prepared by following steps:Take 10 parts of adsorbents, 5 parts of dalcium biphosphates, 6 parts of fermentation beans
The dregs of rice, 15 parts of Testa oryzaes, 15 parts of Semen Maydis powder, 5 parts of rapeseed cakes, 10 parts of wheat brans, 2 parts of saccharomyces cerevisiaes, 1 part of aspergillus niger, mixing and stirring;
Then moisture is adjusted to into 35%~40% with the aqueous solution containing 2% molasses, 3~5d of anaerobic fermentation at 25 DEG C~30 DEG C.
Described adsorbent is zeolite, activated carbon, bentonite, one or more in Vermiculitum.
The present invention provides the technique that a kind of bioanalysises prepare milch cow fermented feed using Salicornia Bigelovii Torr., it is characterised in that concrete to wrap
Include following steps:
(1) batch mixing:In proportion by raw material mix homogeneously, moisture is adjusted to 35%~40% with the aqueous solution containing 2% molasses;
(2) micro- fermentation:By 5~8d of mixed mixture micro- fermentation, periodic agitation;
(3) dispensing:After compost fermentation terminates, quality is proportionally added into than distiller grains 5~10%, lactobacillus powder 2~3%, withered
Careless bacillus cereuss 1~2%, saccharomyces cerevisiae 1~2%, mix homogeneously, with containing 2% molasses aqueous solution adjust moisture to 35%~
40%;
(4) ensiling:28 DEG C~35 DEG C more than silage fermentation 30d of temperature control obtain final product milch cow fermented feed.
The present invention provides the technique that a kind of bioanalysises prepare milch cow fermented feed using Salicornia Bigelovii Torr., and product utilization Salicornia Bigelovii Torr. is
Raw material, is aided with Sargassum slag, alfalfa meal, straw, Chinese herbal medicine etc., is obtained by the two-step fermentation technique of bio-fermentation agent.
The invention provides a kind of bioanalysises prepare the technique of milch cow fermented feed using Salicornia Bigelovii Torr., in milch cow fermented feed
Rich in probiotic bacterias such as yeast, aspergillus niger, bacillus subtilises, saccharomyces cerevisiae, lactic acid bacterias, content up to 2~5 × 108cfu/
G, for milk cattle cultivating after, can significantly improve the immunity of organisms of milch cow, promote growth, improve milk yield, improve milk quality, pole
Tool application and popularization value.
Beneficial effects of the present invention:One is that the present invention adopts the raw material production such as multiple-microorganism composite anaerobic fermentation Salicornia Bigelovii Torr.
Milch cow fermented feed, the natural plant health salt of Salicornia Bigelovii Torr. and natural plant alkaloid (micro- angle saponin), and by with Sargassum slag, lucerne
The synergism of Mu powder, Chinese herbal medicine etc., is obviously improved the raising immunity of milch cow fermented feed, milk of promoting production, improves the work such as milk quality
With;Two is that fermented feed raw material selects Salicornia Bigelovii Torr. etc., and raw material resources amount is big, and low cost is easy to large-scale promotion application Salicornia Bigelovii Torr.
Fermented feed cultural technique;Three is the collective effect of the two-step fermentation and cellulase by complex microorganism, effectively degraded sea
Antinutritional factor in fluffy son, straw, improve the nutritive value of milch cow fermented feed;Four is that present invention process is simple, is had
Beneficial to reduction production cost and industrialized production.
Specific embodiment
With reference to embodiment, the present invention will be further described.
Embodiment 1:
A kind of bioanalysises prepare the technique of milch cow fermented feed using Salicornia Bigelovii Torr., are prepared by following steps:
(1) prepared by bio-fermentation agent:Take 10 parts of adsorbents, 5 parts of dalcium biphosphates, 6 parts of fermented bean cake, 15 parts of Testa oryzaes, 15
Part Semen Maydis powder, 5 parts of rapeseed cakes, 10 parts of wheat brans, 2 parts of saccharomyces cerevisiaes, 1 part of aspergillus niger, mixing and stirring;Then with containing 2%
Moisture is adjusted to 35%~40% by the aqueous solution of molasses, anaerobic fermentation 3d at 25 DEG C~30 DEG C;
(2) batch mixing:By 70 parts of Salicornia Bigelovii Torr. yeast powder, 10 parts of kelp residue, 10 parts of alfalfa meal, 10 parts of corn stalk powder, compound recipe
1 part of Chinese herbal medicine, 2.5 parts of bio-fermentation agent, 1.5 parts of ratio mix homogeneously of cellulase, with the adjustment water of the aqueous solution containing 2% molasses
Divide to 35%~40%;
(3) micro- fermentation:By 5~8d of mixed mixture micro- fermentation, periodic agitation;
(4) dispensing:After compost fermentation terminates, quality is proportionally added into than distiller grains 7.5%, lactobacillus powder 2.5%, hay bud
Spore bacillus 1.5%, saccharomyces cerevisiae 1.5%, mix homogeneously adjust moisture to 35%~40% with containing 2% molasses aqueous solution;
(5) ensiling:28 DEG C~35 DEG C more than silage fermentation 30d of temperature control obtain final product milch cow fermented feed.
Embodiment 2:
A kind of bioanalysises prepare the technique of milch cow fermented feed using Salicornia Bigelovii Torr., are prepared by following steps:
(1) prepared by bio-fermentation agent:Take 10 parts of adsorbents, 5 parts of dalcium biphosphates, 6 parts of fermented bean cake, 15 parts of Testa oryzaes, 15
Part Semen Maydis powder, 5 parts of rapeseed cakes, 10 parts of wheat brans, 2 parts of saccharomyces cerevisiaes, 1 part of aspergillus niger, mixing and stirring;Then with containing 2%
Moisture is adjusted to 35%~40% by the aqueous solution of molasses, anaerobic fermentation 5d at 25 DEG C~30 DEG C;
(2) batch mixing:By 80 parts of Salicornia Bigelovii Torr. yeast powder, 8 parts of horse hair algae-residue, 8 parts of alfalfa meal, 10 parts of wheat stalk powder, compound recipe
1 part of Chinese herbal medicine, 3 parts of bio-fermentation agent, 2 parts of ratio mix homogeneously of cellulase, with the adjustment of the aqueous solution containing 2% molasses moisture extremely
35%~40%;
(3) micro- fermentation:By 5~8d of mixed mixture micro- fermentation, periodic agitation;
(4) dispensing:After compost fermentation terminates, quality is proportionally added into than distiller grains 10%, lactobacillus powder 3%, hay spore
Bacillus 2%, saccharomyces cerevisiae 2%, mix homogeneously adjust moisture to 35%~40% with containing 2% molasses aqueous solution;
(5) ensiling:28 DEG C~35 DEG C more than silage fermentation 30d of temperature control obtain final product milch cow fermented feed.
Embodiment 3:
A kind of bioanalysises prepare the technique of milch cow fermented feed using Salicornia Bigelovii Torr., are prepared by following steps:
(1) prepared by bio-fermentation agent:Take 10 parts of adsorbents, 5 parts of dalcium biphosphates, 6 parts of fermented bean cake, 15 parts of Testa oryzaes, 15
Part Semen Maydis powder, 5 parts of rapeseed cakes, 10 parts of wheat brans, 2 parts of saccharomyces cerevisiaes, 1 part of aspergillus niger, mixing and stirring;Then with containing 2%
Moisture is adjusted to 35%~40% by the aqueous solution of molasses, anaerobic fermentation 4d at 25 DEG C~30 DEG C;
(2) batch mixing:By 60 parts of Salicornia Bigelovii Torr. yeast powder, 12 parts of Sargassum slag, 12 parts of alfalfa meal, 10 parts of rice straw powder, compound recipe
1 part of Chinese herbal medicine, 2 parts of bio-fermentation agent, 1 part of ratio mix homogeneously of cellulase, with the adjustment of the aqueous solution containing 2% molasses moisture extremely
35%~40%;
(3) micro- fermentation:By 5~8d of mixed mixture micro- fermentation, periodic agitation;
(4) dispensing:After compost fermentation terminates, quality is proportionally added into than distiller grains 5~10%, lactobacillus powder 2~3%, withered
Careless bacillus cereuss 1~2%, saccharomyces cerevisiae 1~2%, mix homogeneously, with containing 2% molasses aqueous solution adjust moisture to 35%~
40%;
(5) ensiling:28 DEG C~35 DEG C more than silage fermentation 30d of temperature control obtain final product milch cow fermented feed.
The technique effect that the application is obtained is illustrated below by way of experimental data:
The application milch cow fermented feed is used for into Binzhou and cultivates company, experiment milch cow chooses body weight, parity, lactation period phase
Near healthy holstein cow, each 10 cow head of test group;Conventionally, each test group all same is tried for feeding management
The phase is tested for 6 weeks.Test is grouped as follows:
Table 1 tests grouped table
2 result of the test of table
Above example is merely to illustrate technical scheme, rather than is limited;Although with reference to aforementioned reality
Example is applied to being described in detail by invention, but for the person of ordinary skill of the art, still can be to aforementioned reality
Apply the technical scheme described in example to modify, or equivalent is carried out to which part technical characteristic;And to these modifications
Or replace, do not make the essence of appropriate technical solution depart from the spirit and scope of claimed technical solution of the invention.
Claims (9)
1. a kind of technique that bioanalysises prepare milch cow fermented feed using Salicornia Bigelovii Torr., it is characterised in that the raw material of milch cow fermented feed
Consist of:60~80 parts of Salicornia Bigelovii Torr. yeast powder, 8~12 parts of Sargassum slag, 8~12 parts of alfalfa meal, 10 parts of powder of straw, compound Chinese herb
1 part, 2~3 parts of bio-fermentation agent, 1~2 part of cellulase.
2. the technique that bioanalysises as claimed in claim 1 prepare milch cow fermented feed using Salicornia Bigelovii Torr., it is characterised in that described
Salicornia Bigelovii Torr. yeast powder is prepared by following steps:
(1)Crush immersion:Deimpurity Salicornia Bigelovii Torr. cane will be gone to crush, cross 100 mesh sieves, adjustment Salicornia Bigelovii Torr. cane powder water content 40
~50%;
(2)Enzymolysis:Add the cellulase of Salicornia Bigelovii Torr. quality 1% and 1% non-starch polysaccharide enzyme, 40~60 DEG C of enzymolysis 24h of temperature control;
(3)One-step fermentation:The salt tolerant Alkaliphilic bacillus of inoculation Salicornia Bigelovii Torr. mass ratio 2~3%, in 28~35 DEG C of condition bottom fermentations 3
~5d, stirring in 12 hours is once;
(4)Two-step fermentation:One-step fermentation terminate after the aspergillus niger of inoculation 1~2%, 1~2% saccharomyces cerevisiae, become thoroughly decomposed naturally 30~
50d, obtains final product Salicornia Bigelovii Torr. yeast powder after fermentation ends.
3. the technique that bioanalysises as claimed in claim 1 prepare milch cow fermented feed using Salicornia Bigelovii Torr., it is characterised in that described
Sargassum slag can be Thallus Laminariae (Thallus Eckloniae), sargassum thunbergii, Alga Sgrgassi Enervess, Thallus Sargassi Kjellmaniani, the algae-residue of one or more in Entermorpha.
4. the technique that bioanalysises as claimed in claim 1 prepare milch cow fermented feed using Salicornia Bigelovii Torr., it is characterised in that described
Powder of straw can be Semen Tritici aestivi, Semen Maydiss, one or more in rice straw of mixture.
5. the technique that bioanalysises as claimed in claim 1 prepare milch cow fermented feed using Salicornia Bigelovii Torr., it is characterised in that described
The raw material of compound Chinese herb is consisted of:10 parts of Herba Leonuri, 10 parts of Herb Gynostemmae Pentaphylli, 5 parts of Massa Medicata Fermentata, 5 parts of Herba Houttuyniae, 3 parts of Radix Puerariae, Radix Glycyrrhizae 2
Part.
6. the technique that bioanalysises as claimed in claim 1 prepare milch cow fermented feed using Salicornia Bigelovii Torr., it is characterised in that described
Bio-fermentation agent is prepared by following steps:Take 10 parts of adsorbents, 5 parts of dalcium biphosphates, 6 parts of fermented bean cake, 15 parts of Testa oryzaes, 15
Part Semen Maydis powder, 5 parts of rapeseed cakes, 10 parts of wheat brans, 2 parts of saccharomyces cerevisiaes, 1 part of aspergillus niger, mixing and stirring;Then it is sugared with containing 2%
Moisture is adjusted to 35%~40% by the aqueous solution of honey, 3~5d of anaerobic fermentation at 25 DEG C~30 DEG C.
7. the technique that bioanalysises as claimed in claim 6 prepare milch cow fermented feed using Salicornia Bigelovii Torr., it is characterised in that described
Adsorbent is zeolite, activated carbon, bentonite, one or more in Vermiculitum.
8. the technique that bioanalysises as claimed in claim 1 prepare milch cow fermented feed using Salicornia Bigelovii Torr., it is characterised in that described
Fermenting bed padding is prepared by following steps:
(1)Batch mixing:In proportion by raw material mix homogeneously, moisture is adjusted to 35%~40% with the aqueous solution containing 2% molasses;
(2)Micro- fermentation:By 5~8d of mixed mixture micro- fermentation, periodic agitation;
(3)Dispensing:After compost fermentation terminates, quality is proportionally added into than distiller grains 5~10%, lactobacillus powder 2~3%, hay spore
Bacillus 1~2%, saccharomyces cerevisiae 1~2%, mix homogeneously adjust moisture to 35%~40% with containing 2% molasses aqueous solution;
(4)Ensiling:28 DEG C~35 DEG C more than silage fermentation 30d of temperature control obtain final product milch cow fermented feed.
9. such as the milch cow fermented feed of claim 1-8 preparation.
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