CN106538241A - A kind of Wind-sandy Area Morchella esculenta (L.) Perss artificial cultivation method - Google Patents
A kind of Wind-sandy Area Morchella esculenta (L.) Perss artificial cultivation method Download PDFInfo
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- A—HUMAN NECESSITIES
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Abstract
A kind of Wind-sandy Area Morchella esculenta (L.) Perss artificial cultivation method, comprises the following steps:The first step, successively making parent species, original seed and cultigen;Second step, build for cultivation heliogreenhouse and booth;3rd step, site preparation:Plough with reference to soil before broadcasting, apply substrate of becoming thoroughly decomposed, divide ridge area;4th step, sowing:By trench digging earthing along the line after cultigen uniform broadcasting to ridge face;5th step, field management;6th step, harvesting:Observation cap venation is harvested when clear in time, obtains ripe Morchella esculenta (L.) Perss.The present invention relies on northern Wind-sandy Area wild toadstool resource, by methods such as separate tissue, Spore cultivation or hybrid fusions, the strain of suitable native country growth is tamed out in the culture Jing after strict screening and fruiting experiment, according to scientific formula, using " the three-level production of hybrid seeds ", and increasing shade and micro-sprinkler irrigation system using the facility such as heliogreenhouse and vinyl house, the cultivation under the environmental conditions such as manual control temperature, humidity, illumination and ventilation obtains Morchella esculenta (L.) Perss, has filled up technological gap.
Description
Technical field
The present invention relates to mushroom cultivates field, and in particular to a kind of Wind-sandy Area Morchella esculenta (L.) Perss artificial cultivation method.
Background technology
Morchella esculenta (L.) Perss are that rare famous and precious food (medicine) uses bacterium, with unique local flavor, abundant nutrition and important health-care effect.
, because being affected by environmental factorss such as geographical, weather and soil, seldom, harvesting difficulty is big, and price is occupied always for yield for wild toadstool
It is high not under.Lured by the high selling price of Morchella esculenta (L.) Perss and market prospect, the scientific research personnel that there is many in China begin one's study
The artificial culture of Morchella esculenta (L.) Perss, but mostly rainfall is more, high humidity, winter time is shorter and relatively warmer area obtains into
The ground such as the suitable Sichuan of work(, such as weather conditions, although realize substantially commercialization artificial culture, but Yield and quality is also very not
It is stable.
These local technologies far can not be adapted in the north that dry, intensity of illumination is big, winter time long temperature is low
Just area popularization and application, therefore northern area can't carry out the commercialization cultivation of Morchella esculenta (L.) Perss at present, especially northern Wind-sandy Area.
The content of the invention
Present invention aims to above-mentioned the problems of the prior art, there is provided a kind of Wind-sandy Area Morchella esculenta (L.) Perss artificial culture
Method, it is easy to operation, and cultivated Morchella esculenta (L.) Perss robust growth, yield is high, quality is good, collection period concentrates.
To achieve these goals, the technical solution used in the present invention is comprised the following steps:
The first step, successively making parent species, original seed and cultigen;
1) in annual 4-5 months, harvesting wild toadstool is by the methods such as separate tissue, Spore cultivation or hybrid fusion, Jing
Parent species are obtained after strict screening and fruiting experiment;
2), 30-40 days before sowing, wood flour 60%, Semen Tritici aestivi 20%, Testa Tritici 10%, fertile soil 10% are taken by volume fraction, will
Wood flour, Semen Tritici aestivi and the Testa Tritici for having become thoroughly decomposed in advance is stirred after fully prewetting, adjustment water content to 60%-65%, add fertile soil,
Calx and Gypsum Fibrosum are mixed thoroughly, bottling sterilizing, treat that temperature is down to less than 20 DEG C and is inoculated with parent species, and mycelia is covered with;
3), 15-20 days before sowing, wood flour 55%, Semen Tritici aestivi 35%, fertile soil 10% are taken by volume fraction, is become thoroughly decomposed in advance
Wood flour and Semen Tritici aestivi fully prewet after stir, adjustment water content adds fertile soil, Calx and Gypsum Fibrosum and mixes thoroughly to 60%-65%,
Pack sterilizing, is cooled to less than 20 DEG C and accesses original seed, and mycelia is covered with;
Second step, build for cultivation heliogreenhouse and booth;
3rd step, site preparation:Plough with reference to soil before broadcasting, apply substrate of becoming thoroughly decomposed, divide ridge area;
4th step, sowing:By trench digging earthing along the line after cultigen uniform broadcasting to ridge face;
5th step, field management:Growing environment temperature, humidity, illumination and gas concentration lwevel are precisely controlled, and are mended on schedule
To fuel;
6th step, harvesting:Observation cap venation is harvested when clear in time, obtains ripe Morchella esculenta (L.) Perss.
The concrete steps for cultivating making parent species include:
A. make mother culture media;Rhizoma Solani tuber osi 400g peelings are cut into small pieces and are placed in container, add Testa Tritici 40g, plantation
Native 40g and water 2500ml are heated, and are filtered after boiling Rhizoma Solani tuber osi, are taken filtrate 2000ml and are placed into and be heated in container boiling
Rise, add agar powder 40g, stirring glucose 40g to be added after melting, stop heating, load test tube in time after melting completely
Sterilizing;
B. wild toadstool is separated and prepares parent species;The wild toadstool for harvesting is multiple with aseptic water washing, use 75%
The alcohol disinfecting several seconds is immediately placed in the mercury solution sterilization 1-2 minutes that concentration is 0.1-0.2%, then clean with aseptic water washing, it
Surface moisture is blotted with aseptic filter paper afterwards;Inoculating tool is sterilized, Gaster caprae seu Ovis hyphostroma is cut into small pieces, accessed test tube slant training
Foster base, cultivates under the conditions of being placed in 15-18 DEG C, and timely purification treats that mycelia is covered with test tube and obtains parent species.
Test tube internal diameter 18mm, height 180mm, loading are 1/5-1/4;Bacterium bottle capacity 750ml, a parent species test tube correspondence
Six bacterium bottles of inoculation, mu consumption are 6-8 bottles;Polypropylene Bag of the bacterium bag using 15mm × 28mm, mu consumption are more than 300 bags.
The 5th described step feeds fuel in mycelia vegetative growth phase, and the manufacture method of fuel is:By volume integral
Number takes wood flour 35%, rice husk 25%, Semen Tritici aestivi 30%, fertile soil 10%, and the wood flour for becoming thoroughly decomposed, rice husk and Semen Tritici aestivi are prewetted in advance, adjusts
Whole water content adds fertile soil, Calx, Gypsum Fibrosum and potassium dihydrogen phosphate and mixes thoroughly to 60%-65%, pack sterilizing, obtains nutrition
Material.
The mu consumption of fuel is 1500 jin or so.
The 5th described step vegetative stage keeping temperature is 10 DEG C -20 DEG C, soil moisture is 45%-55%, and son is in fact
The keeping temperature in bulk-growth stage is 8 DEG C -18 DEG C, relative air humidity is 80%-90%, illumination is 500Lux-800Lux, is led to
The concentration of air control carbon dioxide is less than 300ppm.
The row spacing in the ridge area is 80-100cm.
In second step, the method for construction of heliogreenhouse is:Sit 5-7 ° of north orientation south by west and set up body of wall, thing extends, trestle column
8m, span 9m, rear wall height 3.2m, a height of 4.7m of ridge, length 60-80m;Brick wall of the body of wall using inside and outside 24cm, hollow 10-
15cm, external application 10cm thickness styrofoam;Roofing is 15cm thickness color steels, 50 ° of the elevation angle afterwards;Front roof is round pair of string formula galvanized steel of arch
Frame, spacing 1m, 60-70 ° of lighting surface base angle, 30-35 ° of middle and upper part roofing angle;Greenhouse forward position arrange width 20cm, depth 100cm prevent
Han Gou, inside fills out 20cm thickness styrofoams;Front roof covers polyethylene composite film and 700g/m2Space wadding insulation above, is equipped with
Automatic curtain rolling device and film-coiling device;6 pin sunshade net of indoor suspension, supporting installation micro-sprinkler irrigation system.The method of construction of booth is:South
To prolongation, span 8m, length 60-80m, height 3.2m, frame make arch bar and pull bar, bow member spacing using coating steel pipe in the north
9 pin sunshade nets are hung in five road pull bar of 1m, Heng La, canopy, micro-sprinkler irrigation system, canopy group thing arrangement pitches 2m, north and south canopy head are installed
Arch bar, by booth span and bow member spacing embedment clump, is inserted cement by spacing 4m, pre-cast concrete pier, pre-buried zinc-plated pipe fitting
On pier built-in fitting, automatic film reeler is installed on thing both sides, and north and south canopy head is installed into outlet portal.
Compared with prior art, as northern Wind-sandy Area is dry, illumination is strong, the temperature difference is big, winter time length, temperature
Low, extreme temperature reaches less than -30 DEG C, and spring quick heating, summer temp height, thermal extremes are up to more than 35 DEG C, and rainfall is few,
Annual precipitation reaches below 400mm, focus mostly on 7,8, September part.Thermophilic phase soil and air humidity are little, and this natural environment is caused
Wild toadstool distribution is less, rarely has wild toadstool to occur when having rainfall between the month in spring 4-5, is difficult to fruiting when arid is without rain,
Artificial culture can not be carried out.The present invention relies on northern Wind-sandy Area wild toadstool resource, by separate tissue, Spore cultivation or
The methods such as hybrid fusion, the culture Jing after strict screening and fruiting experiment tame out the strain of suitable native country growth, match somebody with somebody according to science
Side, cultivates parent species, original seed and cultigen successively using " the three-level production of hybrid seeds ", and utilizes the facility such as heliogreenhouse and vinyl house
Increase shade and micro-sprinkler irrigation system, create the various cultivation modes under different facilities, in manual control temperature, humidity, illumination
With ventilation etc. under environmental condition cultivation obtain Morchella esculenta (L.) Perss, broken through northern Wind-sandy Area and be unable to artificial culture Morchella esculenta (L.) Perss for a long time
Technical barrier, realizes artificial culture of the Morchella esculenta (L.) Perss in northern Wind-sandy Area, has filled up northern China Wind-sandy Area Morchella esculenta (L.) Perss Planting
The blank of training.The inventive method is reasonable in design, easy to operation, and Morchella esculenta (L.) Perss robust growth, yield are high, quality is good, collection period
Concentrate.
Specific embodiment
With reference to specific embodiment, the present invention is described in further detail.
First, the artificial culture pattern of northern Wind-sandy Area Morchella esculenta (L.) Perss
, by facilities such as heliogreenhouse and vinyl houses, annual 4-5 months, harvesting are wild for the artificial culture of Morchella esculenta (L.) Perss of the present invention
Raw Morchella esculenta (L.) Perss cultivate this by methods such as separate tissue, Spore cultivation or hybrid fusions Jing after strict screening and fruiting experiment
The morchella mother culture of soilization.
Cultivation in Greenhouse makes original seed mid-September then, and early October makes cultigen, and late October is sowed, and 11
The first tenday period of a month moon are mended for one time of nutrition material, and 2 months next year early and middle ten dayses start fruiting, and harvesting terminates in the past by the end of March.Vinyl house survive the winter stubble cultivation
Train and original seed is made mid-August then, early September makes cultigen, late September sowing, October early and middle ten dayses are mended and supply one time of nutrition
Material, mid-March in next year, soil thawed, and started to strengthen management, and late April starts fruiting, and late May harvesting terminates;Vinyl house
Early spring stubble is cultivated and makes original seed in late December, makes cultigen mid-January, and early Febuary sowing, late Febuary are mended for once seeking
Nutriment, late April start fruiting, and early June harvesting terminates.
2nd, the design and construction method of northern Wind-sandy Area Morchella esculenta (L.) Perss cultivating facility
1st, heliogreenhouse design and construction method
(1) heliogreenhouse site planning:Heliogreenhouse is built and should select that landform is open, physical features is flat, has abundant water resources, power supply
It is stable, have a good transport service where, can the suitable bare place construction of prioritizing selection, occupied cultivated land with reducing.
(2) design parameter:Heliogreenhouse sits north orientation south by west 5-7 degree, and thing extends, trestle column 8m;Greenhouse is semi-underground
Formula, span 9m, rear wall height 3.2m, a height of 4.7m of ridge, length 60-80m;Wall body structure is inside and outside 24cm brick walls, hollow 10-
15cm, external application 10cm thickness styrofoam;Roofing is 15cm thickness color steels, 50 ° or so of the elevation angle afterwards;Front roof is round pair of string formula plating of arch
Zinc steelframe, spacing 1m, 60-70 ° of lighting surface base angle, 30-35 ° of middle and upper part roofing angle;Greenhouse forward position arranges width 20cm, depth 100cm
Cold-proof Ditch, inside fill out 20cm thickness styrofoam;Front roof insulation covers polyethylene multifunctional composite film and 700g/m2Space above
Cotton-wadded quilt, is equipped with automatic curtain rolling device and film-coiling device;6 pin sunshade net of sunlight indoor suspension, supporting installation micro-sprinkler irrigation system.
(3) building technology:Heliogreenhouse will have a set of regular design drawing before building, in the case where technical staff instructs by special
Industry crew constructs.Wall is played after the compacting wall base, brick staggeredly will draw inside and outside body of wall.Front roof steelframe is smooth vertical and horizontal, solid up and down,
Roofing wants that smooth seamless, draining is good, load is good afterwards.Front roof clear covering selects polyethylene multifunctional composite film, protects
Adiabator selects space wadding quilt, and supporting installation automatic curtain system, film-coiling device and sunshade net and micro-sprinkler irrigation system.
2nd, vinyl house design and construction method
It is span 8m that vinyl house builds parameter, and length 60-80m, height 3.2m, North and South direction extend.Frame adopts ф
20 coating steel pipes do arch bar, and 15 coating steel pipes of ф do
Net, installs micro-sprinkler irrigation system.Vinyl house place election is identical with greenhouse, canopy group arrangement thing spacing 2m, north and south canopy head spacing
4m.Building technology is to build first prefabricated 20 × 30cm clumps before canopy, while 15 zinc-plated pipe fittings of pre-buried Φ, by booth span and bow member
Spacing imbeds clump, and arch bar is inserted on the built-in fitting of clump, and five road pull bar on top and waist, thing both sides are installed certainly
Dynamic film-coiling device, north and south canopy head are installed into outlet portal, with secondary installation canopy film, micro-sprinkler irrigation system, hang sunshade net.
3rd, northern Wind-sandy Area Morchella esculenta (L.) Perss strain manufacture method
Strain of the present invention prepares strain using three-level producing method for seed, while preparing benefit supplies fuel, manufacture method is respectively such as
Under:
1st, the manufacture method of parent species
(1) mother culture media method for production:
1. needed raw material:Rhizoma Solani tuber osi 400g, agar powder 40g, glucose 40g, Testa Tritici 40g, planting soil 40g, water 2500ml.
2. method for production:Peeling potatoes are cut into small pieces and are placed in pot, add Testa Tritici, planting soil and water, boil filtration, will
Filtrate pours boiled in pot, addition agar powder into, stirs to thawing and adds glucose, stops heating after all melting.Take advantage of
The test tube of heat 18 × 180mm of injection, charge weight are advisable for the 1/5-1/4 of test tube height, are disposed vertically, and load sterilizing in pressure cooker
Take out afterwards and be put into inclined-plane, can use after vapor in test tube is done naturally.
(2) Morchella esculenta (L.) Perss separation method:During wild toadstool fruiting, a harvesting pork thickness, the fresh open country of strong age of good looking appearance
Raw Morchella esculenta (L.) Perss, with aseptic water washing several times, with 75% alcohol disinfecting several seconds after, be immediately placed in the hydrargyrum that concentration is 0.1-0.2% molten
Liquid disinfectant 1-2 minutes, then it is clean with aseptic water washing, surface moisture is blotted with aseptic filter paper afterwards;Inoculating tool is gone out
Bacterium, Gaster caprae seu Ovis hyphostroma are cut into small pieces, and access in test tube slant culture medium, cultivate, timely purification, bacterium under the conditions of being placed in 15-18 DEG C
The full test tube of filament length obtains parent species.
2nd, the manufacture method of original seed
(1) original seed Production Time:30-40 days before sowing.
(2) original seed production quantity:Every mu of 6-8 bottle.
(3) original seed formula:Wood flour 60%, Semen Tritici aestivi 20%, Testa Tritici 10%, fertile soil 10%, Calx and appropriate Gypsum Fibrosum.
(4) original seed manufacture method:Wood flour becomes thoroughly decomposed in advance, and Semen Tritici aestivi is using front being soaked.
Stir after first the wood flour for having become thoroughly decomposed, Semen Tritici aestivi and Testa Tritici fully being prewetted when making original seed, adjustment water content to 60%-
65%, add fertile soil, quick lime and Gypsum Fibrosum and mix thoroughly, bottling sterilizing can inoculation after cooling.Inoculation must below 20 DEG C ring
Border is carried out, inoculation of preferably sterilizing in inoculating hood.One parent species can be inoculated with six bottles of original seeds, it is finished after be put into temperature for 15-20
DEG C, breezy indoor cultivation, mycelia is covered with and obtains final product original seed within about 15-20 days.
3rd, the manufacture method of cultigen
(1) Production Time:Sow first -20 days 15 days.
(2) production quantity:300 bags per mu (15 × 28 Polypropylene Bag).
(3) formula:Wood flour 55%, Semen Tritici aestivi 35%, detritus soil 10%, Calx and appropriate Gypsum Fibrosum.
The same original seed of manufacture method of cultigen.
4th, mend the manufacture method for fuel
(1) Production Time:After planting 5-20 days, flexibly determine according to temperature and field mycelial growth situation.
(2) production quantity:1500 jin per mu.
(3) formula:Wood flour 35%, rice husk 25%, Semen Tritici aestivi 30%, fertile soil 10%, Calx and Gypsum Fibrosum and potassium dihydrogen phosphate are suitable
Amount.
(4) manufacture method:Wood flour, rice husk and Semen Tritici aestivi are prewetted in advance and soaked, adjustment water content is mixed thoroughly to 60%-65%
Fertile soil and Calx, Gypsum Fibrosum and potassium dihydrogen phosphate are added, can be used after pack sterilizing cooling.
4th, northern Wind-sandy Area Morchella esculenta (L.) Perss artificial cultivation method
1st, site preparation:Plough with reference to soil before broadcasting, apply the dedicated substrate for having become thoroughly decomposed in right amount, then divide ridge area, row spacing 80-
100cm。
2nd, sow:By 300 bag/mu sowing quantities by strain uniform broadcasting in ridge face, earthing of ditching along the line immediately, furrow width
The soil that trench digging digs out is overlying on ridge face by 30cm, depth 20cm, and thickness of earth covering 3-5cm, earthing of also mechanical can ditching once are completed.
3rd, after planting manage
(1) after planting open spraying system and spray a water, black mulch film is covered when temperature is down to below 10 degree in facility,
Pour permeable before overlay film, mulch film can not be obturaged reality, it is to avoid mycelia anoxia.
Whole trophophase will temperature and moisture under diligent observation control film, it is ensured that not hydrops not hydropenia, and will avoid high temperature.
(2) second day mycelia after broadcasting starts to sprout, and covers with substantially ridge face within one week or so, need to mend for one time of nutrition material.Nutrition
The benefit of material is after planting 5-20 days for the time, 1500 jin or so of mu consumption.
4th, the regulation and control of facilities environment condition
(1) water management:Soil conservation moisture state to be made before sowing, " kind water " after planting will be sprayed once, soil plough horizon exists
More than 30cm will be irrigated, and with backsight Changes in weather situation and soil moisture content, see that dry stool is sprayed water, to meet the moisture need of hyphal development
Ask, it is 2-3 days before harvesting, appropriate to control water to ensure commodity property.
(2) temperature treatment:The vegetative stage of Morchella esculenta (L.) Perss maintains the temperature at 10-20 DEG C, not more than 25 DEG C, and son is real
Bulk-growth stage holding temperature at 8-18 DEG C, not more than 20 DEG C.When ambient temperature is more than 20 DEG C, to add a cover outside canopy straw screen or mat or
Cotton-wadded quilt, shading cooling, during more than 25 DEG C, around facility can be installed spraying apparatus and spray in fine day noon, in case temperature of shed
The too high growth for affecting Morchella esculenta (L.) Pers sporophore.
(3) illumination management:Morchella esculenta (L.) Pers. Mycelium is suitably grown under faint light condition, but appropriate light is to sporophore shape
500-800Lux is generally kept in into illumination during having certain facilitation, fruiting.
(4) ventilating management:Morchella aerobic funguses, will keep ventilation state, control gas concentration lwevel not to surpass as far as possible
300ppm is crossed, the management that combines with control of temperature and humidity of divulging information.
5th, harvest:Morchella esculenta (L.) Pers sporophore be unearthed after 10-15 days, just growth and maturity when cap venation is clear will be harvested in time,
It is close to prescind near the ground from stem base portion with sharp pocket knife during harvesting, the fresh mushroom after harvesting cuts soil in time, cases cold
Hide fresh-keeping, marketing fresh or sale can be dried (dry).Crimp or breakage are reduced, keeps a type satisfactorily complete, prevent moisture regain life
It is mould.
Claims (8)
1. a kind of Wind-sandy Area Morchella esculenta (L.) Perss artificial cultivation method, it is characterised in that comprise the following steps:
The first step, successively making parent species, original seed and cultigen;
1) annual 4-5 months, harvesting wild toadstool is screened and is gone out by separate tissue, Spore cultivation or hybrid fusion
Parent species are obtained after mushroom test;
2), 30-40 days before sowing, wood flour 60%, Semen Tritici aestivi 20%, Testa Tritici 10%, fertile soil 10% are taken by volume fraction, will be prior
Wood flour, Semen Tritici aestivi and the Testa Tritici for having become thoroughly decomposed is stirred after fully prewetting, and adjustment water content adds fertile soil, Calx to 60%-65%
Mix thoroughly with Gypsum Fibrosum, bottling sterilizing treats that temperature is down to less than 20 DEG C and accesses parent species, and mycelia is covered with;
3), 15-20 days before sowing, wood flour 55%, Semen Tritici aestivi 35%, fertile soil 10% are taken by volume fraction, by the wood for having become thoroughly decomposed in advance
Bits are stirred after fully being prewetted with Semen Tritici aestivi, and adjustment water content adds fertile soil, Calx and Gypsum Fibrosum and mixes thoroughly to 60%-65%, packs
Sterilizing, treats that temperature is down to less than 20 DEG C and accesses original seed, and mycelia is covered with;
Second step, build for cultivation heliogreenhouse and booth;
3rd step, site preparation:Plough with reference to soil before broadcasting, apply substrate of becoming thoroughly decomposed, divide ridge area;
4th step, sowing:By trench digging earthing along the line after cultigen uniform broadcasting to ridge face;
5th step, field management:Control growing environment temperature, humidity, illumination and gas concentration lwevel simultaneously feed fuel on schedule;
6th step, harvesting:Observation cap venation is harvested when clear in time, obtains ripe Morchella esculenta (L.) Perss.
2. Morchella esculenta (L.) Perss artificial cultivation method in Wind-sandy Area according to claim 1, it is characterised in that cultivate the tool for making parent species
Body step includes:A. make mother culture media;Rhizoma Solani tuber osi 400g peelings are cut into small pieces and are placed in container, added Testa Tritici 40g, plant
Plant soil 40g and water 2500ml to boil 30 minutes, filter, filtrate is placed into and be heated in container boiling, add agar powder
40g, stirring add glucose 40g after melting, and stop heating after melting completely, load test tube sterilizing in time;B. wild sheep
Tripe bacterium separates and prepares parent species;The wild toadstool for harvesting is multiple with aseptic water washing, stood with 75% alcohol disinfecting several seconds
The mercury solution sterilization 1-2 minutes that concentration is 0.1%-0.2% are put into, then it is clean with aseptic water washing, aseptic filter paper is used afterwards
Blot surface moisture;Inoculating tool is sterilized, Gaster caprae seu Ovis hyphostroma is cut into small pieces, access in test tube slant culture medium, be placed in
Cultivate under the conditions of 15 DEG C -18 DEG C, timely purification treats that mycelia is covered with test tube and obtains parent species.
3. Morchella esculenta (L.) Perss artificial cultivation method in Wind-sandy Area according to claim 2, it is characterised in that:Described test tube internal diameter is
18mm, is highly 180mm, and loading is 1/5-1/4;Described bacterium bottle capacity is 750ml, parent species test tube correspondence inoculation six
Individual bacterium bottle, mu consumption are 6-8 bottles;Polypropylene Bag of the bacterium bag using 15mm × 28mm, mu consumption are more than 300 bags.
4. Morchella esculenta (L.) Perss artificial cultivation method in Wind-sandy Area according to claim 1, it is characterised in that the 5th described step is in bacterium
Silk vegetative growth phase feeds fuel, and the manufacture method of fuel is:
Wood flour 35%, rice husk 25%, Semen Tritici aestivi 30%, fertile soil 10% are taken by volume fraction, Calx, Gypsum Fibrosum and potassium dihydrogen phosphate are fitted
Amount, the wood flour for becoming thoroughly decomposed, rice husk and Semen Tritici aestivi are fully prewetted stirring, adjust water content to 60%-65%, add fertile soil, stone
Ash, Gypsum Fibrosum and potassium dihydrogen phosphate are mixed thoroughly, pack sterilizing, and fuel, 1500 jin of mu consumption are obtained after cooling.
5. Morchella esculenta (L.) Perss artificial cultivation method in Wind-sandy Area according to claim 1, it is characterised in that the 5th step mycelial growth rank
10 DEG C -20 DEG C of keeping temperature of section, soil moisture 45%-55%, 8 DEG C -18 DEG C of sporophore growth stage holding temperature, air humidity
80%-90%, illumination 500Lux-800Lux, Ventilation Control gas concentration lwevel are less than 300ppm.
6. Morchella esculenta (L.) Perss artificial cultivation method in Wind-sandy Area according to claim 1, it is characterised in that:Row spacing 80-100cm.
7. Morchella esculenta (L.) Perss artificial cultivation method in Wind-sandy Area according to claim 1, it is characterised in that heliogreenhouse in second step
Method of construction be:Sit 5-7 ° of north orientation south by west and set up body of wall, thing extends, trestle column 8m, span 9m, rear wall height 3.2m,
The a height of 4.7m of ridge, length 60-80m;Brick wall of the body of wall using inside and outside 24cm, hollow 10-15cm, external application 10cm thick styrofoam;
Roofing is 15cm thick color steel, 50 ° of the elevation angle afterwards;Front roof is round pair of string formula zinc-plated steel frame of arch, spacing 1m, lighting surface base angle
60-70 °, 30-35 ° of middle and upper part roofing angle;Greenhouse forward position arranges width 20cm, the Cold-proof Ditch of depth 100cm, inside fills out 20cm thickness polyphenyl
Plate;Front roof covers polyethylene composite film and 700g/m2Space wadding insulation above, is equipped with automatic curtain rolling device and wound membrane
Device;6 pin sunshade net of indoor suspension, supporting installation micro-sprinkler irrigation system.
8. Morchella esculenta (L.) Perss artificial cultivation method in Wind-sandy Area according to claim 1, it is characterised in that booth builds in second step
The method of making is:North and South direction extends, and span 8m, length 60-80m, height 3.2m, frame make arch bar and drawing using coating steel pipe
9 pin sunshade nets are hung in bar, bow member spacing 1m, five road pull bars of Heng La, canopy, micro-sprinkler irrigation system, canopy group's thing arrangement pitches are installed
2m, north and south canopy head spacing 4m, pre-cast concrete pier, pre-buried zinc-plated pipe fitting, by booth span and bow member spacing embedment clump, will arch
On bar insertion clump built-in fitting, automatic film reeler is installed on thing both sides, and north and south canopy head is installed into outlet portal.
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Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101878720A (en) * | 2010-07-01 | 2010-11-10 | 杨国伟 | Method for domesticating and cultivating wild mushroom |
CN101926262A (en) * | 2010-04-16 | 2010-12-29 | 董毅 | Morel indoor cultivation method and greenhouse thereof |
CN103819281A (en) * | 2014-03-25 | 2014-05-28 | 成都天绿菌业有限公司 | Preparation method of culture material formula and nutrition pack formula for morel |
CN105248148A (en) * | 2015-10-12 | 2016-01-20 | 李正娟 | Culture method for improving Morchella extracellular polysaccharide content |
CN205196488U (en) * | 2015-12-15 | 2016-05-04 | 郝哲 | Helio -greenhouse |
CN105613042A (en) * | 2016-01-25 | 2016-06-01 | 四川保兴现代农业科技股份有限公司 | Breeding method for liquid strain of morchella and industrial cultivation method for morchella |
CN205305513U (en) * | 2016-01-27 | 2016-06-15 | 驻马店市农业科学院 | Flat canopy suitable for cultivation hickory chick |
CN205305514U (en) * | 2016-01-27 | 2016-06-15 | 驻马店市农业科学院 | Canopy encircles suitable for cultivation hickory chick |
CN105917965A (en) * | 2016-05-20 | 2016-09-07 | 四川省农业科学院土壤肥料研究所 | Garden under-forest cultivation method for Morchella |
-
2016
- 2016-10-31 CN CN201610934171.0A patent/CN106538241A/en active Pending
Patent Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101926262A (en) * | 2010-04-16 | 2010-12-29 | 董毅 | Morel indoor cultivation method and greenhouse thereof |
CN101878720A (en) * | 2010-07-01 | 2010-11-10 | 杨国伟 | Method for domesticating and cultivating wild mushroom |
CN103819281A (en) * | 2014-03-25 | 2014-05-28 | 成都天绿菌业有限公司 | Preparation method of culture material formula and nutrition pack formula for morel |
CN105248148A (en) * | 2015-10-12 | 2016-01-20 | 李正娟 | Culture method for improving Morchella extracellular polysaccharide content |
CN205196488U (en) * | 2015-12-15 | 2016-05-04 | 郝哲 | Helio -greenhouse |
CN105613042A (en) * | 2016-01-25 | 2016-06-01 | 四川保兴现代农业科技股份有限公司 | Breeding method for liquid strain of morchella and industrial cultivation method for morchella |
CN205305513U (en) * | 2016-01-27 | 2016-06-15 | 驻马店市农业科学院 | Flat canopy suitable for cultivation hickory chick |
CN205305514U (en) * | 2016-01-27 | 2016-06-15 | 驻马店市农业科学院 | Canopy encircles suitable for cultivation hickory chick |
CN105917965A (en) * | 2016-05-20 | 2016-09-07 | 四川省农业科学院土壤肥料研究所 | Garden under-forest cultivation method for Morchella |
Non-Patent Citations (3)
Title |
---|
杨玉红等: "《生命科学综合实验指导》", 31 July 2016, 东北大学出版社 * |
潘熙筑: "《食用菌栽培实用技术问答》", 31 October 2006, 科学技术文献出版社 * |
郝哲等: "北方风沙区羊肚菌人工栽培技术研究初探", 《中国食用菌》 * |
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