CN106536704A - 核酸扩增装置、核酸扩增方法以及核酸扩增用芯片 - Google Patents
核酸扩增装置、核酸扩增方法以及核酸扩增用芯片 Download PDFInfo
- Publication number
- CN106536704A CN106536704A CN201580036603.XA CN201580036603A CN106536704A CN 106536704 A CN106536704 A CN 106536704A CN 201580036603 A CN201580036603 A CN 201580036603A CN 106536704 A CN106536704 A CN 106536704A
- Authority
- CN
- China
- Prior art keywords
- pcr
- nucleic acid
- microchannel
- acid amplification
- sample solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000003199 nucleic acid amplification method Methods 0.000 title claims abstract description 68
- 230000003321 amplification Effects 0.000 title claims abstract description 42
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 41
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 40
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 40
- 238000003753 real-time PCR Methods 0.000 claims abstract description 58
- 239000007788 liquid Substances 0.000 claims abstract description 55
- 239000012488 sample solution Substances 0.000 claims abstract description 48
- 238000000137 annealing Methods 0.000 claims abstract description 21
- 239000000758 substrate Substances 0.000 claims abstract description 16
- 238000004925 denaturation Methods 0.000 claims abstract description 8
- 230000036425 denaturation Effects 0.000 claims abstract description 8
- 238000000034 method Methods 0.000 claims description 58
- 108090000623 proteins and genes Proteins 0.000 claims description 39
- 238000006243 chemical reaction Methods 0.000 claims description 25
- 238000003757 reverse transcription PCR Methods 0.000 claims description 20
- 238000001514 detection method Methods 0.000 claims description 15
- 230000008859 change Effects 0.000 claims description 12
- 238000007403 mPCR Methods 0.000 claims description 11
- 238000005259 measurement Methods 0.000 claims description 11
- 238000005452 bending Methods 0.000 claims description 10
- 238000004458 analytical method Methods 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 6
- 230000008520 organization Effects 0.000 claims description 5
- 239000011159 matrix material Substances 0.000 claims description 3
- 102000004190 Enzymes Human genes 0.000 claims description 2
- 108090000790 Enzymes Proteins 0.000 claims description 2
- 238000001914 filtration Methods 0.000 claims description 2
- 238000006116 polymerization reaction Methods 0.000 claims 1
- 230000007246 mechanism Effects 0.000 abstract 5
- 238000003752 polymerase chain reaction Methods 0.000 description 97
- 108020004414 DNA Proteins 0.000 description 75
- 239000000243 solution Substances 0.000 description 37
- 239000000523 sample Substances 0.000 description 35
- 238000010839 reverse transcription Methods 0.000 description 21
- 241001263478 Norovirus Species 0.000 description 20
- 230000002441 reversible effect Effects 0.000 description 19
- 230000004087 circulation Effects 0.000 description 16
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 15
- 238000012360 testing method Methods 0.000 description 14
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 11
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 11
- 238000005516 engineering process Methods 0.000 description 11
- 239000007850 fluorescent dye Substances 0.000 description 11
- 230000007850 degeneration Effects 0.000 description 9
- 108010085238 Actins Proteins 0.000 description 8
- 102100034343 Integrase Human genes 0.000 description 8
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 8
- 208000015181 infectious disease Diseases 0.000 description 8
- 230000008569 process Effects 0.000 description 8
- 241000606153 Chlamydia trachomatis Species 0.000 description 7
- 208000035473 Communicable disease Diseases 0.000 description 6
- 108020004635 Complementary DNA Proteins 0.000 description 6
- 241000588724 Escherichia coli Species 0.000 description 6
- 241000588652 Neisseria gonorrhoeae Species 0.000 description 6
- 238000010804 cDNA synthesis Methods 0.000 description 6
- 239000002299 complementary DNA Substances 0.000 description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 6
- 238000002372 labelling Methods 0.000 description 6
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 201000010099 disease Diseases 0.000 description 5
- 230000001965 increasing effect Effects 0.000 description 5
- 101150019793 G2 gene Proteins 0.000 description 4
- 108091028043 Nucleic acid sequence Proteins 0.000 description 4
- 238000005276 aerator Methods 0.000 description 4
- 230000002068 genetic effect Effects 0.000 description 4
- 238000007689 inspection Methods 0.000 description 4
- 230000014759 maintenance of location Effects 0.000 description 4
- 238000007789 sealing Methods 0.000 description 4
- 230000035945 sensitivity Effects 0.000 description 4
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 3
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 3
- 241000725303 Human immunodeficiency virus Species 0.000 description 3
- 206010028980 Neoplasm Diseases 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 238000011088 calibration curve Methods 0.000 description 3
- 201000011510 cancer Diseases 0.000 description 3
- 206010022000 influenza Diseases 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 244000000010 microbial pathogen Species 0.000 description 3
- 239000013612 plasmid Substances 0.000 description 3
- 239000011347 resin Substances 0.000 description 3
- 229920005989 resin Polymers 0.000 description 3
- 230000001225 therapeutic effect Effects 0.000 description 3
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 2
- 102000053602 DNA Human genes 0.000 description 2
- 208000035217 Ring chromosome 1 syndrome Diseases 0.000 description 2
- 108020004682 Single-Stranded DNA Proteins 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 238000012790 confirmation Methods 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 230000001351 cycling effect Effects 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 239000003085 diluting agent Substances 0.000 description 2
- 241001493065 dsRNA viruses Species 0.000 description 2
- 230000005284 excitation Effects 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 230000008676 import Effects 0.000 description 2
- 239000000787 lecithin Substances 0.000 description 2
- 229940067606 lecithin Drugs 0.000 description 2
- 235000010445 lecithin Nutrition 0.000 description 2
- 238000013507 mapping Methods 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 229920000098 polyolefin Polymers 0.000 description 2
- 230000035484 reaction time Effects 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 230000003252 repetitive effect Effects 0.000 description 2
- 238000012827 research and development Methods 0.000 description 2
- 230000000717 retained effect Effects 0.000 description 2
- 238000005728 strengthening Methods 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- MPLHNVLQVRSVEE-UHFFFAOYSA-N texas red Chemical compound [O-]S(=O)(=O)C1=CC(S(Cl)(=O)=O)=CC=C1C(C1=CC=2CCCN3CCCC(C=23)=C1O1)=C2C1=C(CCC1)C3=[N+]1CCCC3=C2 MPLHNVLQVRSVEE-UHFFFAOYSA-N 0.000 description 2
- 238000013518 transcription Methods 0.000 description 2
- 230000035897 transcription Effects 0.000 description 2
- 108700026220 vif Genes Proteins 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 108020004465 16S ribosomal RNA Proteins 0.000 description 1
- 102000007469 Actins Human genes 0.000 description 1
- PLXMOAALOJOTIY-FPTXNFDTSA-N Aesculin Natural products OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@H](O)[C@H]1Oc2cc3C=CC(=O)Oc3cc2O PLXMOAALOJOTIY-FPTXNFDTSA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 208000003322 Coinfection Diseases 0.000 description 1
- 101150039808 Egfr gene Proteins 0.000 description 1
- 241000588722 Escherichia Species 0.000 description 1
- 208000007212 Foot-and-Mouth Disease Diseases 0.000 description 1
- 241000710198 Foot-and-mouth disease virus Species 0.000 description 1
- 206010018612 Gonorrhoea Diseases 0.000 description 1
- 206010019799 Hepatitis viral Diseases 0.000 description 1
- 239000005411 L01XE02 - Gefitinib Substances 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 108091005461 Nucleic proteins Proteins 0.000 description 1
- 208000001388 Opportunistic Infections Diseases 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 206010061902 Pancreatic neoplasm Diseases 0.000 description 1
- 238000011529 RT qPCR Methods 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 239000004411 aluminium Substances 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 238000003149 assay kit Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 239000003183 carcinogenic agent Substances 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 229940038705 chlamydia trachomatis Drugs 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 230000009429 distress Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000010291 electrical method Methods 0.000 description 1
- 238000003487 electrochemical reaction Methods 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 102000052116 epidermal growth factor receptor activity proteins Human genes 0.000 description 1
- 108700015053 epidermal growth factor receptor activity proteins Proteins 0.000 description 1
- 108700021358 erbB-1 Genes Proteins 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000001917 fluorescence detection Methods 0.000 description 1
- 238000002795 fluorescence method Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- XGALLCVXEZPNRQ-UHFFFAOYSA-N gefitinib Chemical compound C=12C=C(OCCCN3CCOCC3)C(OC)=CC2=NC=NC=1NC1=CC=C(F)C(Cl)=C1 XGALLCVXEZPNRQ-UHFFFAOYSA-N 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 208000001786 gonorrhea Diseases 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 229940084651 iressa Drugs 0.000 description 1
- 238000002032 lab-on-a-chip Methods 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 208000015486 malignant pancreatic neoplasm Diseases 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000004089 microcirculation Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- YOHYSYJDKVYCJI-UHFFFAOYSA-N n-[3-[[6-[3-(trifluoromethyl)anilino]pyrimidin-4-yl]amino]phenyl]cyclopropanecarboxamide Chemical compound FC(F)(F)C1=CC=CC(NC=2N=CN=C(NC=3C=C(NC(=O)C4CC4)C=CC=3)C=2)=C1 YOHYSYJDKVYCJI-UHFFFAOYSA-N 0.000 description 1
- 102000042567 non-coding RNA Human genes 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 238000012946 outsourcing Methods 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- NRNCYVBFPDDJNE-UHFFFAOYSA-N pemoline Chemical compound O1C(N)=NC(=O)C1C1=CC=CC=C1 NRNCYVBFPDDJNE-UHFFFAOYSA-N 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 239000010453 quartz Substances 0.000 description 1
- 238000010791 quenching Methods 0.000 description 1
- 230000000171 quenching effect Effects 0.000 description 1
- 108700042226 ras Genes Proteins 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 238000011897 real-time detection Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012340 reverse transcriptase PCR Methods 0.000 description 1
- 239000003161 ribonuclease inhibitor Substances 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 235000014347 soups Nutrition 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000012085 test solution Substances 0.000 description 1
- 238000005382 thermal cycling Methods 0.000 description 1
- 230000036962 time dependent Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 241001529453 unidentified herpesvirus Species 0.000 description 1
- 241000712461 unidentified influenza virus Species 0.000 description 1
- 201000001862 viral hepatitis Diseases 0.000 description 1
- 230000009385 viral infection Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
- B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0654—Lenses; Optical fibres
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0883—Serpentine channels
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
- B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/70—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in livestock or poultry
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Clinical Laboratory Science (AREA)
- Immunology (AREA)
- Dispersion Chemistry (AREA)
- Hematology (AREA)
- Medicinal Chemistry (AREA)
- Sustainable Development (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
Claims (11)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910720912.9A CN110452808B (zh) | 2014-07-08 | 2015-07-07 | 核酸扩增装置、核酸扩增方法以及核酸扩增用芯片 |
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2014-140758 | 2014-07-08 | ||
JP2014140758 | 2014-07-08 | ||
PCT/JP2015/069549 WO2016006612A1 (ja) | 2014-07-08 | 2015-07-07 | 核酸増幅装置、核酸増幅方法及び核酸増幅用チップ |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201910720912.9A Division CN110452808B (zh) | 2014-07-08 | 2015-07-07 | 核酸扩增装置、核酸扩增方法以及核酸扩增用芯片 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN106536704A true CN106536704A (zh) | 2017-03-22 |
CN106536704B CN106536704B (zh) | 2020-03-06 |
Family
ID=55064241
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201910720912.9A Active CN110452808B (zh) | 2014-07-08 | 2015-07-07 | 核酸扩增装置、核酸扩增方法以及核酸扩增用芯片 |
CN201580036603.XA Active CN106536704B (zh) | 2014-07-08 | 2015-07-07 | 核酸扩增装置、核酸扩增方法以及核酸扩增用芯片 |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201910720912.9A Active CN110452808B (zh) | 2014-07-08 | 2015-07-07 | 核酸扩增装置、核酸扩增方法以及核酸扩增用芯片 |
Country Status (6)
Country | Link |
---|---|
US (2) | US11098347B2 (zh) |
EP (1) | EP3168287A4 (zh) |
JP (8) | JP6226284B2 (zh) |
CN (2) | CN110452808B (zh) |
SG (2) | SG10201811430SA (zh) |
WO (1) | WO2016006612A1 (zh) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107083426A (zh) * | 2017-03-30 | 2017-08-22 | 杭州晶格科学仪器有限公司 | 一种荧光定量检测方法 |
CN111742223A (zh) * | 2017-12-11 | 2020-10-02 | 株式会社百奥尼 | 聚合酶链反应*** |
CN113574161A (zh) * | 2019-03-15 | 2021-10-29 | 国立研究开发法人产业技术综合研究所 | 核酸扩增方法 |
Families Citing this family (45)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US11098347B2 (en) * | 2014-07-08 | 2021-08-24 | National Institute Of Advanced Industrial Science And Technology | Nucleic acid amplification device, nucleic acid amplification method, and chip for nucleic acid amplification |
JP6561209B2 (ja) * | 2016-05-18 | 2019-08-14 | 日本板硝子株式会社 | 反応処理装置および反応処理装置の制御方法 |
US10510436B2 (en) | 2016-07-05 | 2019-12-17 | Credo Biomedical Pte Ltd. | Using serial dilutions of reference samples to construct a reference table for sigmoidal fitting in real-time PCR copy number analysis |
RU2739951C2 (ru) * | 2016-11-01 | 2020-12-30 | Ниппон Шит Глас Кампани, Лимитед | Реакционный сосуд и реакционное устройство, предназначенные для проведения полимеразной цепной реакции |
WO2018105404A1 (ja) * | 2016-12-06 | 2018-06-14 | 日本板硝子株式会社 | 反応処理装置 |
JP2018108055A (ja) * | 2017-01-05 | 2018-07-12 | 東洋紡株式会社 | ノロウイルスg2型を検出するためのオリゴヌクレオチドプローブ |
WO2018151246A1 (ja) * | 2017-02-16 | 2018-08-23 | 東洋紡株式会社 | Rnaを検出する方法およびrnaを検出するための試薬 |
CN110603315B (zh) * | 2017-06-06 | 2022-08-09 | 日本板硝子株式会社 | 反应处理装置 |
CN107604056B (zh) * | 2017-09-18 | 2021-06-08 | 星源智(珠海)生物科技有限公司 | 一种核酸测定方法 |
WO2020014400A1 (en) * | 2018-07-10 | 2020-01-16 | Gen-Probe Incorporated | Methods and systems for detecting and quantifying nucleic acids |
FR3088340B1 (fr) * | 2018-11-12 | 2021-01-22 | Commissariat Energie Atomique | Systeme automatise de preparation, de detection et d'analyse d'un echantillon fluidique |
WO2020129116A1 (ja) * | 2018-12-17 | 2020-06-25 | 日本板硝子株式会社 | 反応処理装置、反応処理容器および反応処理方法 |
JP2020146305A (ja) * | 2019-03-14 | 2020-09-17 | 株式会社三洋物産 | 遊技機 |
JP2020146303A (ja) * | 2019-03-14 | 2020-09-17 | 株式会社三洋物産 | 遊技機 |
JP2020146310A (ja) * | 2019-03-14 | 2020-09-17 | 株式会社三洋物産 | 遊技機 |
JP2020146308A (ja) * | 2019-03-14 | 2020-09-17 | 株式会社三洋物産 | 遊技機 |
JP2020146302A (ja) * | 2019-03-14 | 2020-09-17 | 株式会社三洋物産 | 遊技機 |
JP2020146301A (ja) * | 2019-03-14 | 2020-09-17 | 株式会社三洋物産 | 遊技機 |
JP2020146316A (ja) * | 2019-03-14 | 2020-09-17 | 株式会社三洋物産 | 遊技機 |
JP2020146317A (ja) * | 2019-03-14 | 2020-09-17 | 株式会社三洋物産 | 遊技機 |
JP2020146297A (ja) * | 2019-03-14 | 2020-09-17 | 株式会社三洋物産 | 遊技機 |
JP2020146304A (ja) * | 2019-03-14 | 2020-09-17 | 株式会社三洋物産 | 遊技機 |
JP2020146299A (ja) * | 2019-03-14 | 2020-09-17 | 株式会社三洋物産 | 遊技機 |
JP2020146307A (ja) * | 2019-03-14 | 2020-09-17 | 株式会社三洋物産 | 遊技機 |
JP2020146313A (ja) * | 2019-03-14 | 2020-09-17 | 株式会社三洋物産 | 遊技機 |
JP2020146314A (ja) * | 2019-03-14 | 2020-09-17 | 株式会社三洋物産 | 遊技機 |
JP2020146315A (ja) * | 2019-03-14 | 2020-09-17 | 株式会社三洋物産 | 遊技機 |
JP2020146298A (ja) * | 2019-03-14 | 2020-09-17 | 株式会社三洋物産 | 遊技機 |
JP2020146306A (ja) * | 2019-03-14 | 2020-09-17 | 株式会社三洋物産 | 遊技機 |
JP2020146309A (ja) * | 2019-03-14 | 2020-09-17 | 株式会社三洋物産 | 遊技機 |
JP2020146312A (ja) * | 2019-03-14 | 2020-09-17 | 株式会社三洋物産 | 遊技機 |
JP2020146300A (ja) * | 2019-03-14 | 2020-09-17 | 株式会社三洋物産 | 遊技機 |
JP2020146311A (ja) * | 2019-03-14 | 2020-09-17 | 株式会社三洋物産 | 遊技機 |
JP7341492B2 (ja) * | 2019-04-05 | 2023-09-11 | 株式会社ゴーフォトン | 反応処理容器、反応処理容器の製造方法および反応処理方法 |
CN109971617A (zh) * | 2019-04-30 | 2019-07-05 | 郭嘉杰 | 一种pcr扩增装置的低温处理*** |
US11465143B2 (en) | 2019-06-07 | 2022-10-11 | Nippon Sheet Glass Company, Limited | Reaction processing vessel |
CN110628607A (zh) * | 2019-10-10 | 2019-12-31 | 上海纽钛测控技术有限公司 | 适合突发事件现场应用的高效恒温pcr装置及实现方法 |
JP7417794B2 (ja) * | 2019-12-10 | 2024-01-19 | 杏林製薬株式会社 | 核酸増幅方法、核酸増幅装置及び核酸増幅用チップ |
WO2022034866A1 (ja) | 2020-08-11 | 2022-02-17 | 杏林製薬株式会社 | 核酸増幅チップ |
JPWO2022034867A1 (zh) | 2020-08-11 | 2022-02-17 | ||
CN112322472B (zh) * | 2020-11-05 | 2022-07-12 | 上海交通大学 | 一种适用于核酸检测的即时检测装置 |
JPWO2022153999A1 (zh) * | 2021-01-14 | 2022-07-21 | ||
WO2022219758A1 (ja) * | 2021-04-14 | 2022-10-20 | 株式会社日立ハイテク | サンプルのpcr解析を実行するためのデバイス、pcr反応装置、pcrシステムおよびpcr方法 |
JP2024086296A (ja) * | 2022-12-16 | 2024-06-27 | 国立研究開発法人産業技術総合研究所 | 核酸増幅用チップ、核酸増幅装置、および核酸増幅方法 |
JP7393070B1 (ja) | 2023-08-04 | 2023-12-06 | 株式会社ゴーフォトン | Pcr方法 |
Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2005065607A (ja) * | 2003-08-26 | 2005-03-17 | Hitachi Ltd | 遺伝子処理チップおよび遺伝子処理装置 |
CN1680574A (zh) * | 2004-03-12 | 2005-10-12 | 三星电子株式会社 | 用于扩增核酸的方法及设备 |
JP2007101200A (ja) * | 2005-09-30 | 2007-04-19 | Yokogawa Electric Corp | 化学反応用カートリッジおよびその使用方法 |
JP2009517075A (ja) * | 2005-11-30 | 2009-04-30 | デルタドット・リミテッド | 標識を利用しない固有のイメージングを用いたリアルタイムpcrのモニタリング |
JP2011117805A (ja) * | 2009-12-02 | 2011-06-16 | Beckman Coulter Inc | マイクロ流体チップ |
US20120178091A1 (en) * | 2011-01-06 | 2012-07-12 | Meso Scale Technologies, Llc | Assay Cartridges and Methods of Using the Same |
US20140005066A1 (en) * | 2012-06-29 | 2014-01-02 | Advanced Liquid Logic Inc. | Multiplexed PCR and Fluorescence Detection on a Droplet Actuator |
Family Cites Families (42)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP3515646B2 (ja) | 1995-09-18 | 2004-04-05 | 大塚電子株式会社 | マルチキャピラリ電気泳動装置 |
JP2002014100A (ja) | 2000-06-29 | 2002-01-18 | Kanegafuchi Chem Ind Co Ltd | 反応チップ用基板およびこれから作製した反応チップ |
FR2831081B1 (fr) | 2001-10-24 | 2004-09-03 | Commissariat Energie Atomique | Dispositif d'injection parallelisee et synchronisee pour injections sequentielles de reactifs differents |
US7094379B2 (en) | 2001-10-24 | 2006-08-22 | Commissariat A L'energie Atomique | Device for parallel and synchronous injection for sequential injection of different reagents |
US7041481B2 (en) * | 2003-03-14 | 2006-05-09 | The Regents Of The University Of California | Chemical amplification based on fluid partitioning |
CA2479452C (en) | 2003-08-30 | 2008-11-04 | F.Hoffmann-La Roche Ag | Method and device for determining analytes in a liquid |
JP2005204678A (ja) | 2004-01-19 | 2005-08-04 | Terumo Corp | プレフィルドシリンジ製造方法、プレフィルドシリンジ製造用治具およびプレフィルドシリンジ製造装置 |
KR100906749B1 (ko) * | 2004-03-25 | 2009-07-09 | (주)바이오니아 | 인터컬레이팅 형광염료가 표지된 프로브를 이용한 핵산 증폭 측정 방법 |
JP3952036B2 (ja) | 2004-05-13 | 2007-08-01 | コニカミノルタセンシング株式会社 | マイクロ流体デバイス並びに試液の試験方法および試験システム |
JP2006271216A (ja) | 2005-03-28 | 2006-10-12 | Dainippon Ink & Chem Inc | 特定の塩基配列を有する核酸の有無を判定するシステムおよび判定方法 |
CA2606750C (en) | 2005-05-11 | 2015-11-24 | Nanolytics, Inc. | Method and device for conducting biochemical or chemical reactions at multiple temperatures |
ES2393758T3 (es) * | 2006-03-15 | 2012-12-27 | Micronics, Inc. | Ensayos integrados de ácidos nucleicos |
JP4685691B2 (ja) | 2006-04-13 | 2011-05-18 | 株式会社日立ソリューションズ | 検査チップ及び検査チップシステム |
JP4903518B2 (ja) | 2006-08-22 | 2012-03-28 | 三菱エンジニアリングプラスチックス株式会社 | 蛍光検出分析基板用芳香族ポリカーボネート樹脂組成物および蛍光検出分析基板 |
US8263392B2 (en) * | 2006-11-14 | 2012-09-11 | University Of Utah Research Foundation | Methods and compositions related to continuous flow thermal gradient PCR |
EP2090781B1 (en) | 2006-12-09 | 2018-08-22 | Murata Manufacturing Co. Ltd. | Piezoelectric micro-blower |
JP5036377B2 (ja) * | 2007-04-09 | 2012-09-26 | 株式会社日立ソリューションズ | 反応装置及び反応チップ |
JP5104097B2 (ja) | 2007-07-27 | 2012-12-19 | 株式会社村田製作所 | 流体移送装置 |
JP4957480B2 (ja) | 2007-09-20 | 2012-06-20 | 株式会社村田製作所 | 圧電マイクロポンプ |
JP5224801B2 (ja) * | 2007-12-21 | 2013-07-03 | キヤノン株式会社 | 核酸増幅装置 |
JPWO2009113356A1 (ja) | 2008-03-12 | 2011-07-21 | コニカミノルタエムジー株式会社 | 反応検出装置 |
JP5303983B2 (ja) | 2008-03-26 | 2013-10-02 | 株式会社島津製作所 | 反応処理方法及び反応処理装置 |
JPWO2009125676A1 (ja) | 2008-04-09 | 2011-08-04 | コニカミノルタエムジー株式会社 | 検査システム |
EP3073114B1 (en) | 2008-06-03 | 2018-07-25 | Murata Manufacturing Co., Ltd. | Piezoelectric micro-blower |
EP2312158B1 (en) | 2008-06-05 | 2016-04-27 | Murata Manufacturing Co. Ltd. | Piezoelectric microblower |
RU2385940C1 (ru) * | 2008-10-23 | 2010-04-10 | Общество с ограниченной ответственностью "ВИНТЕЛ" | Способ определения нуклеиновых кислот методом полимеразно-цепной реакции в режиме реального времени и устройство для его осуществления |
JP5310373B2 (ja) | 2009-05-14 | 2013-10-09 | ソニー株式会社 | 光学的検出装置 |
US20110065101A1 (en) | 2009-06-04 | 2011-03-17 | Lockheed Martin Corporation | Multiple-sample microfluidic chip for DNA analysis |
JP5277214B2 (ja) | 2010-07-27 | 2013-08-28 | 株式会社日立ハイテクノロジーズ | 自動分析装置 |
EP2611942A4 (en) | 2010-08-31 | 2015-01-14 | Canon Us Life Sciences Inc | ANTI-TURNING AT TEMPERATURE CHANGING |
US9114399B2 (en) | 2010-08-31 | 2015-08-25 | Canon U.S. Life Sciences, Inc. | System and method for serial processing of multiple nucleic acid assays |
EP2441520A1 (en) | 2010-10-12 | 2012-04-18 | Eppendorf AG | Real-time amplification and micro-array based detection of nucleic acid targets in a flow chip assay |
US10131903B2 (en) * | 2011-04-01 | 2018-11-20 | The Regents Of The University Of California | Microfluidic platform for synthetic biology applications |
EP2699700B8 (en) * | 2011-04-20 | 2016-08-24 | Mesa Biotech, Inc. | Integrated device for nucleic acid detection and identification |
JP2013055921A (ja) | 2011-09-09 | 2013-03-28 | National Institute Of Advanced Industrial Science & Technology | 核酸増幅方法 |
JP5211336B2 (ja) | 2012-02-16 | 2013-06-12 | 株式会社メトラン | ポンプユニット、呼吸補助装置 |
WO2013132645A1 (ja) * | 2012-03-09 | 2013-09-12 | 独立行政法人産業技術総合研究所 | 核酸増幅方法 |
JP6075722B2 (ja) | 2012-06-21 | 2017-02-08 | ハンファテクウィン株式会社Hanwha Techwin Co.,Ltd. | 作業機械 |
WO2014024608A1 (ja) | 2012-08-10 | 2014-02-13 | 株式会社村田製作所 | ブロア |
US10550917B2 (en) | 2013-03-14 | 2020-02-04 | Cordell E. Ebeling | Slide-glide privacy blind barrier system |
WO2015019520A1 (ja) * | 2013-08-08 | 2015-02-12 | パナソニック株式会社 | マイクロ流体デバイス |
US11098347B2 (en) | 2014-07-08 | 2021-08-24 | National Institute Of Advanced Industrial Science And Technology | Nucleic acid amplification device, nucleic acid amplification method, and chip for nucleic acid amplification |
-
2015
- 2015-07-07 US US15/322,000 patent/US11098347B2/en active Active
- 2015-07-07 CN CN201910720912.9A patent/CN110452808B/zh active Active
- 2015-07-07 SG SG10201811430SA patent/SG10201811430SA/en unknown
- 2015-07-07 CN CN201580036603.XA patent/CN106536704B/zh active Active
- 2015-07-07 WO PCT/JP2015/069549 patent/WO2016006612A1/ja active Application Filing
- 2015-07-07 EP EP15818467.1A patent/EP3168287A4/en active Pending
- 2015-07-07 SG SG11201610707RA patent/SG11201610707RA/en unknown
- 2015-07-07 JP JP2016532945A patent/JP6226284B2/ja active Active
-
2017
- 2017-09-26 JP JP2017184881A patent/JP6519888B2/ja active Active
-
2018
- 2018-07-25 JP JP2018139765A patent/JP6460362B2/ja active Active
- 2018-11-22 JP JP2018219281A patent/JP6530548B2/ja active Active
-
2019
- 2019-05-16 JP JP2019092559A patent/JP6765659B2/ja active Active
-
2020
- 2020-02-05 US US16/782,794 patent/US11781181B2/en active Active
- 2020-09-09 JP JP2020150918A patent/JP6996717B2/ja active Active
-
2021
- 2021-11-30 JP JP2021194200A patent/JP7250292B2/ja active Active
-
2023
- 2023-03-13 JP JP2023038348A patent/JP2023075250A/ja active Pending
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2005065607A (ja) * | 2003-08-26 | 2005-03-17 | Hitachi Ltd | 遺伝子処理チップおよび遺伝子処理装置 |
CN1680574A (zh) * | 2004-03-12 | 2005-10-12 | 三星电子株式会社 | 用于扩增核酸的方法及设备 |
JP2007101200A (ja) * | 2005-09-30 | 2007-04-19 | Yokogawa Electric Corp | 化学反応用カートリッジおよびその使用方法 |
JP2009517075A (ja) * | 2005-11-30 | 2009-04-30 | デルタドット・リミテッド | 標識を利用しない固有のイメージングを用いたリアルタイムpcrのモニタリング |
JP2011117805A (ja) * | 2009-12-02 | 2011-06-16 | Beckman Coulter Inc | マイクロ流体チップ |
US20120178091A1 (en) * | 2011-01-06 | 2012-07-12 | Meso Scale Technologies, Llc | Assay Cartridges and Methods of Using the Same |
US20140005066A1 (en) * | 2012-06-29 | 2014-01-02 | Advanced Liquid Logic Inc. | Multiplexed PCR and Fluorescence Detection on a Droplet Actuator |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107083426A (zh) * | 2017-03-30 | 2017-08-22 | 杭州晶格科学仪器有限公司 | 一种荧光定量检测方法 |
CN111742223A (zh) * | 2017-12-11 | 2020-10-02 | 株式会社百奥尼 | 聚合酶链反应*** |
CN113574161A (zh) * | 2019-03-15 | 2021-10-29 | 国立研究开发法人产业技术综合研究所 | 核酸扩增方法 |
Also Published As
Publication number | Publication date |
---|---|
CN110452808A (zh) | 2019-11-15 |
CN110452808B (zh) | 2022-11-22 |
SG11201610707RA (en) | 2017-01-27 |
JP6765659B2 (ja) | 2020-10-07 |
JP2022033853A (ja) | 2022-03-02 |
JPWO2016006612A1 (ja) | 2017-04-27 |
SG10201811430SA (en) | 2019-01-30 |
US11098347B2 (en) | 2021-08-24 |
JP2023075250A (ja) | 2023-05-30 |
EP3168287A1 (en) | 2017-05-17 |
US20170130261A1 (en) | 2017-05-11 |
JP6530548B2 (ja) | 2019-06-12 |
US20200157607A1 (en) | 2020-05-21 |
CN106536704B (zh) | 2020-03-06 |
JP6519888B2 (ja) | 2019-05-29 |
JP6226284B2 (ja) | 2017-11-08 |
JP6996717B2 (ja) | 2022-02-03 |
EP3168287A4 (en) | 2018-01-24 |
JP2019047812A (ja) | 2019-03-28 |
JP2018183172A (ja) | 2018-11-22 |
JP7250292B2 (ja) | 2023-04-03 |
JP2021000106A (ja) | 2021-01-07 |
WO2016006612A1 (ja) | 2016-01-14 |
US11781181B2 (en) | 2023-10-10 |
JP2018033460A (ja) | 2018-03-08 |
JP2019150050A (ja) | 2019-09-12 |
JP6460362B2 (ja) | 2019-01-30 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US11781181B2 (en) | Nucleic acid amplification device, nucleic acid amplification method, and chip for nucleic acid amplification | |
JP7490610B2 (ja) | 核酸分析および定量化のための方法ならびにシステム | |
Jin et al. | Rapid detection of foodborne bacterial pathogens using visual high‐throughput microfluidic chip | |
CN106687598B (zh) | 检测样品中多种核苷酸的方法 | |
JP5178149B2 (ja) | 標的核酸のリアルタイムpcr方法及びその装置 | |
JP2011062197A (ja) | 生体試料定量方法 | |
CN113574161A (zh) | 核酸扩增方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
TA01 | Transfer of patent application right | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20170921 Address after: Japan's Tokyo Kasumigaseki Chiyoda a chome 3 times 1 Applicant after: National Research and Development Corporation Industrial Technology Comprehensive Research Institute Applicant after: Jettas Co. Address before: Japan's Tokyo Kasumigaseki Chiyoda a chome 3 times 1 Applicant before: National Research and Development Corporation Industrial Technology Comprehensive Research Institute |
|
TA01 | Transfer of patent application right |
Effective date of registration: 20171221 Address after: Japan's Tokyo Kasumigaseki Chiyoda a chome 3 times 1 Applicant after: National Research and Development Corporation Industrial Technology Comprehensive Research Institute Applicant after: KYORIN PHARMACEUTICAL Co.,Ltd. Address before: Japan's Tokyo Kasumigaseki Chiyoda a chome 3 times 1 Applicant before: National Research and Development Corporation Industrial Technology Comprehensive Research Institute Applicant before: Jettas Co. |
|
TA01 | Transfer of patent application right | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
CP03 | Change of name, title or address |
Address after: Japan's Tokyo Kasumigaseki Chiyoda a chome 3 times 1 Patentee after: National Research and Development Corporation Industrial Technology Comprehensive Research Institute Country or region after: Japan Patentee after: KYORIN PHARMACEUTICAL Co.,Ltd. Address before: Japan's Tokyo Kasumigaseki Chiyoda a chome 3 times 1 Patentee before: National Research and Development Corporation Industrial Technology Comprehensive Research Institute Country or region before: Japan Patentee before: Xinglin Holdings Co.,Ltd. |
|
CP03 | Change of name, title or address | ||
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20240123 Address after: Japan's Tokyo Kasumigaseki Chiyoda a chome 3 times 1 Patentee after: National Research and Development Corporation Industrial Technology Comprehensive Research Institute Country or region after: Japan Patentee after: Xinglin Holdings Co.,Ltd. Address before: Japan's Tokyo Kasumigaseki Chiyoda a chome 3 times 1 Patentee before: National Research and Development Corporation Industrial Technology Comprehensive Research Institute Country or region before: Japan Patentee before: KYORIN PHARMACEUTICAL Co.,Ltd. |