CN106520585B - The production method of high-content mannosan yeast culture - Google Patents
The production method of high-content mannosan yeast culture Download PDFInfo
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Abstract
The invention discloses a kind of production methods of high-content mannosan yeast culture, belong to technical field of bioengineering.Saccharomyces cerevisiae seed liquor is seeded in liquid fermentation medium first, 30~32 DEG C of 18~20h of aerobic culture keep thallus horizontal up to high density, cultivation temperature is down to 24~26 DEG C again, and into fermentation system, stream adds hydrogen peroxide to 6~10mmol/L of final concentration simultaneously, continue to cultivate, fermentation culture is mixed with solid medium with 3:6~3:8 weight ratio again, it is transferred in solid state fermentation case after Anaerobic culturel 24~28 hours, low temperature drying, it crushes and can get this product, its mannan content reaches 4.0~4.5%, and water-solubility protein reaches 7%~9%.High-content mannosan yeast culture of the invention can dramatically increase 1~2kg/ of Lactation of Dairy Cow amount (head ﹒ days), milk cow somatic cell count is effectively reduced, and significantly improve milk cow health situation, be conducive to drinking person health.
Description
Technical field
The present invention relates to a kind of production methods of high-content mannosan yeast culture, belong to biotechnology neck
Domain.
Background technique
Yeast culture typically refers to what S. cervisiae obtained under special process and nutritional condition by sufficiently fermentation
Compound criteria object, the culture are mainly made of three parts: yeast thallus, yeast metabolism product, denaturation nutrient matrix, these
Rich in effective components and the part unknown function factors such as mannosan, glucan, amino acid, B family vitamin, nucleic acid in component.
As functional fodder raw material, yeast culture has effects that significant disease-resistant, volume increase, can micro- life into animal alimentary canal
Object provides nutriment, so that micro-ecological environment in their metabolic activity and liptinite is excited, with antibiotics additive phase
Than having many advantages, such as green natural, having no toxic side effect and palatability is good, full of nutrition, be therefore widely used in livestock and poultry and support
Grow field.
The function and its mechanism of yeast culture are main are as follows: improve gastrointestinal bacterial flora and fall structure, improve breeding performonce fo animals；
It supplements the nutrients, improves digestive function, improve immunity of organisms and premunition.
Mannosan is the key function factor of yeast culture, it can be dramatically increased, and animal body fluid is immune and cellular immunity
Ability adjusts intestinal flora balance, in conjunction with the exogenous pathogen of absorption, selective absorption bacteriotoxin, and has anti-radiation, anti-
Oxidation, antitumor isoreactivity function.In consideration of it, Ministry of Agriculture's on December 19th, 2013 issues No. 2038 bulletin, by " saccharomyces cerevisiae
Culture " is transferred to " feedstuff catalogue " from " catalogue of feed additive varieties ", and mannosan is mandatory as the product
Mark requires index, and mannosan has some idea of to the importance of Cultures of S. cerevisiae.
Mannosan is located on the outside of yeast cell wall, and yeast cell wall is typical sandwich structure, intermediate course be by
Protein composition, two sides are caused by mannosan and beta glucan and fraction chitin, phosphatide and fiber substance respectively
At three-dimensional space net structure, yeast mannans account for about the 10-20% of yeast dry weight for close conclusion.The content of yeast mannans
It is significantly affected by external environment, Liu Hongzhi etc. has found system of the mannan content by Yeast Cultivation trophic factor under study for action
About；Ni Jingyue etc. has found that condition of culture significantly affects the content of mannosan under study for action.
Although mannosan is the key function factor of yeast culture, yeast culture production technology pertinent literature
In be but rarely reported, the technical research of current yeast culture focuses primarily upon in the selection of cheap substrates, after all mainly
It is in order to reduce cost, turn waste into wealth, as Chinese patent (application number: 201410076270.0) is reported using yellow wine lees as matrix
Carry out the preparation of yeast culture；And Chinese patent (application number: 201510319359.X) report using distillers ' grains as matrix into
The mobile fermentation of row hygrometric state yeast culture；In addition, Chinese patent (application number: 201510578518.8) is reported to mix grain
Slag is the fermenting and producing that raw material carries out yeast culture.
Saccharomyces cerevisiae is amphimicrobe, is suitable for various culture environments, but the conversion of its substrate and product generation in different environments
The approach of thanking is not quite similar, and in order to make yeast culture by sufficiently fermentation, fermentation process usually merges liquid-solid and ferments, is good
A variety of training modes such as oxygen-anaerobic fermentation.How in the fermentation process of a variety of training modes fusion, pass through modulation process parameter
It has important practical significance to improve mannan content for the production technology innovation of yeast culture.
Summary of the invention
The technical problem to be solved by the invention is to provide a kind of production method of high-content mannosan yeast culture,
Mannan content reaches 4.0~4.5% in the yeast culture produced using the technique, and water-solubility protein reaches 7%~
9%.
In order to solve the above technical problems, the technical scheme adopted by the invention is that:
A kind of production method of high-content mannosan yeast culture, comprising the following steps:
Step a, the preparation of saccharomyces cerevisiae seed liquor: 1~2 ring of saccharomyces cerevisiae strain of picking slant preservation is seeded to kind
In sub- culture medium, in shaking table shaken cultivation, saccharomyces cerevisiae seed liquor is obtained；
Step b, saccharomyces cerevisiae liquid High Density Cultivation: saccharomyces cerevisiae seed liquor is seeded to equipped with liquid state fermentation culture medium
Reactor tank in cultivate under Yu Zhongwen, weakly acidic condition；
Step c, high-content mannosan saccharomyces cerevisiae low temperature and hydrogen peroxide coercing cultivation: the reaction temperature of reactor tank is reduced
Degree, while the hydrogen peroxide of 15%~25% mass concentration at the uniform velocity stream being added in reactor tank, until the end of hydrogen peroxide is dense in reactor tank
Degree reaches 6~10mmol/L, then proceedes to cultivate several hours, obtains fermentation culture；
Step d, saccharomyces cerevisiae solid-state Anaerobic culturel: fermentation culture and solid medium are mixed according to certain weight ratio
It closes, obtains mixture, mixture is then transferred to solid state fermentation case Anaerobic culturel under closed obstructed gaseity, is cultivated
Object；
Step e, low temperature drying: culture is transferred in drying device, is dried culture using low temperature hot wind,
Obtain dry yeast culture；
Step f, crushing and packaging: dry yeast culture being crushed, is packaged into bag, is protected from light storage in a cool and dry place.
Technical solution of the present invention further improvement lies in that: in step a saccharomyces cerevisiae strain be CICC1355, saccharomyces cerevisiae
The seed culture medium each component and its weight percent of strain are as follows: 0.5~1% yeast extract, 0.5~1% peptone, 0.5~
1% bean cake powder, sucrose 1~2%, remaining is purified water.
Technical solution of the present invention further improvement lies in that: in step a the temperature of shaking table shaken cultivation be 30~32 DEG C, vibration
Swing that revolving speed is 200~250rpm, the shaken cultivation time is 16~18h.
Technical solution of the present invention further improvement lies in that: the liquid state fermentation culture medium of saccharomyces cerevisiae is each in the step b
Component and its weight percent are as follows: 0.5~1% peptone, 1.5~2% bean cake powders, 1~1.5% corn flour, molasses 2~4%,
Remaining is purified water.
Technical solution of the present invention further improvement lies in that: reactor tank interior reaction temperature is 30~32 DEG C in step b, pH is
5.0~6.0, speed of agitator is 150~250rpm, Ventilation Rate is 0.5~1.0vvm, incubation time is 18~20h.
Technical solution of the present invention further improvement lies in that: reactor tank interior reaction temperature is reduced to 24~26 in step c
DEG C, while being added to hydrogen peroxide at the uniform velocity stream in reactor tank by peristaltic pump；Continuing incubation time is 4~6 hours.
Technical solution of the present invention further improvement lies in that: the weight of fermentation culture and solid medium is mixed in step d
Composition and division in a proportion is 3:6~3:8, each component and its weight percent of solid medium are as follows: 15~35% bean cake powders, 5~10% corns
Powder, 55~80% wheat brans.
Technical solution of the present invention further improvement lies in that: in step d the solid state fermentation case Anaerobic culturel time be 24~28
Hour, the temperature of solid state fermentation room is 30~32 DEG C in solid state fermentation case.
Technical solution of the present invention further improvement lies in that: the temperature of low temperature hot wind is 50 DEG C~70 DEG C in step e, dry
The water content of yeast culture is 13% hereinafter, dry yeast culture mannan content reaches 4~4.5%, water-soluble egg
It is white to reach 7%~9%.
Technical solution of the present invention further improvement lies in that: dry yeast culture is crushed in step f using pulverizer
At 40~60 mesh granularities, packing machine is packaged into bag, and Packing Unit is 10~50Kg/ bags.
By adopting the above-described technical solution, the technological progress achieved by the present invention is:
The object of the present invention is to provide a kind of production methods of high-content mannosan yeast culture.Entire production process
Using liquid it is aerobic-by the way of solid-state anaerobism cultivates stage by stage；The low temperature that uses in the liquid later period in aerobic fermentation stage and double
Oxygen water oxygen coercing cultivation is conducive to the formation of high-content mannosan saccharomyces cerevisiae, the yeast culture produced using the technique
Middle mannan content reaches 4.0~4.5%, and water-solubility protein reaches 7%~9%.CICC1355 purchase therein is in China
Research for Industrial Microbial Germ preservation administrative center belongs to the Research for Industrial Microbial Germ in " Chinese microorganism strain catalogue ", bacterium number
1355 Chinese of CICC: saccharomyces cerevisiae plants name: Saccharomyces, generic name cerevisiae, purposes: fodder yeast.
The principle of the present invention: firstly, the aerobic high density fermentation mode of the liquid used early period provides foot for follow-up phase
The microbial cell of enough amounts；Then the low temperature and hydrogen peroxide oxidation coercing cultivation used is conducive to the wine brewing of high-content mannosan
The formation of yeast；The solid anaerobic digestion of final stage is conducive to yeast and sufficiently ferments the more abundant functional components of synthesis.
After fermentation, solid state fermentation culture is subjected to low temperature drying, crushed, is i.e. acquisition finished product.
The seed culture medium each component and its weight percent of saccharomyces cerevisiae strain in step a are as follows: 0.5~1% yeast leaching
Cream, 0.5~1% peptone, 0.5~1% bean cake powder, sucrose 1~2%, remaining is purified water.Configuration is simple, and nutritive value is high；
The liquid state fermentation culture medium each component and its weight percent of saccharomyces cerevisiae in step b are as follows: 0.5~1% peptone, 1.5~2%
Bean cake powder, 1~1.5% corn flour, molasses 2~4%, remaining is purified water, and configuration is simple, full of nutrition, wherein the sugar selected
Honey is sticky one kind, dark brown, in semifluid object, mainly contains sucrose, and pantothenic acid content is higher in cane molasses, reaches 37mg/
Kg, furthermore biological cellulose content is also very considerable.Molasses are the byproducts of sugar industry, and group origin cause of formation sugaring raw material, processing conditions are not
It is same and variant, wherein mainly containing a large amount of fermentable sugars (mainly sucrose), thus it is good fermentation raw material, can be used as ferment
The substrate or base-material of the fermented products such as mother, monosodium glutamate, organic acid can be used as the raw material and animal feed of certain food.
Low temperature and hydrogen peroxide oxidation coercing cultivation are conducive to the formation of high-content mannosan saccharomyces cerevisiae, and liquid is aerobic-
The mode of solid-state anaerobism multi-mode culture stage by stage is conducive to yeast and sufficiently ferments the more abundant functional components of synthesis.This hair
Bright technology stability is good, no mould contamination, product safety；Nutritive value is high；Simple process, at low cost, easily operated, quality
Stablize, production process environmental protection, non-environmental-pollution, parameter is easily controllable, and fermentation efficiency and fermented quality are high.
Specific embodiment
A kind of production method of high-content mannosan yeast culture, comprising the following steps:
Step a, the preparation of saccharomyces cerevisiae seed liquor: 1~2 ring of saccharomyces cerevisiae strain of picking slant preservation is seeded to kind
In sub- culture medium, in shaking table shaken cultivation, saccharomyces cerevisiae seed liquor is obtained；
Step b, saccharomyces cerevisiae liquid High Density Cultivation: saccharomyces cerevisiae seed liquor is seeded to equipped with liquid state fermentation culture medium
Reactor tank in cultivate under Yu Zhongwen, weakly acidic condition；
Step c, high-content mannosan saccharomyces cerevisiae low temperature and hydrogen peroxide coercing cultivation: the reaction temperature of reactor tank is reduced
Degree, while the hydrogen peroxide of 15%~25% mass concentration at the uniform velocity stream being added in reactor tank, until the end of hydrogen peroxide is dense in reactor tank
Degree reaches 6~10mmol/L, then proceedes to cultivate several hours, obtains fermentation culture；
Step d, saccharomyces cerevisiae solid-state Anaerobic culturel: fermentation culture and solid medium are mixed according to certain weight ratio
It closes, obtains mixture, mixture is then transferred to solid state fermentation case Anaerobic culturel under closed obstructed gaseity, is cultivated
Object；
Step e, low temperature drying: culture is transferred in drying device, is dried culture using low temperature hot wind,
Obtain dry yeast culture；
Step f, crushing and packaging: dry yeast culture being crushed, is packaged into bag, is protected from light storage in a cool and dry place.
Saccharomyces cerevisiae strain is CICC1355, the seed culture medium each component and its weight of saccharomyces cerevisiae strain in step a
Percentage are as follows: 0.5~1% yeast extract, 0.5~1% peptone, 0.5~1% bean cake powder, sucrose 1~2%, remaining is purifying
Water.
The temperature of shaking table shaken cultivation is 30~32 DEG C in step a, oscillation revolving speed is 200~250rpm, shaken cultivation when
Between be 16~18h.
The liquid state fermentation culture medium each component and its weight percent of saccharomyces cerevisiae in the step b are as follows: 0.5~1% egg
White peptone, 1.5~2% bean cake powders, 1~1.5% corn flour, molasses 2~4%, remaining is purified water.
Reactor tank interior reaction temperature is 30~32 DEG C in step b, pH is 5.0~6.0, speed of agitator be 150~250rpm,
Ventilation Rate is 0.5~1.0vvm, incubation time is 18~20h.
Reactor tank interior reaction temperature is reduced to 24~26 DEG C in step c, while at the uniform velocity being flowed hydrogen peroxide by peristaltic pump
It adds in reactor tank；Continuing incubation time is 4~6 hours.
The Mixing ratio by weight of fermentation culture and solid medium is 3:6~3:8, each group of solid medium in step d
Part and its weight percent are as follows: 15~35% bean cake powders, 5~10% corn flour, 55~80% wheat brans.
The solid state fermentation case Anaerobic culturel time is 24~28 hours in step d, the temperature of solid state fermentation room in solid state fermentation case
Degree is 30~32 DEG C.
The temperature of low temperature hot wind is 50 DEG C~70 DEG C in step e, and the water content of dry yeast culture is 13% hereinafter, dry
Dry yeast culture mannan content reaches 4~4.5%, and water-solubility protein reaches 7%~9%.
Dry yeast culture is ground into 40~60 mesh granularities using pulverizer in step f, packing machine is packaged into
Bag, Packing Unit are 10~50Kg/ bags.
The present invention is described in further details below with reference to embodiment:
Embodiment one:
A kind of production method of high-content mannosan yeast culture, comprising the following steps:
Step a, the preparation of yeast starter liquid: 2 ring of saccharomyces cerevisiae (CICC1355) strain of picking slant preservation is seeded to
In seed culture medium, 30 DEG C, 200rpm shaking table shaken cultivation 18h.
Step b, saccharomyces cerevisiae liquid High Density Cultivation: seed culture fluid is seeded to equipped with liquid state fermentation culture medium
In 1000 liters of reactor tanks, 30 DEG C of temperature, control pH are in 5.0~6.0 ranges in tank, speed of agitator 200rpm, Ventilation Rate
1.0vvm cultivates 20h.
Step c, high-content mannosan saccharomyces cerevisiae low temperature and hydrogen peroxide coercing cultivation: by the temperature of step b fermentation system
Degree is reduced to 24 DEG C, while being added to the hydrogen peroxide of 20% mass concentration at the uniform velocity stream in reactor tank by peristaltic pump, until hydrogen peroxide
Final concentration reaches 8mmol/L, continues culture 6 hours.
Step d, saccharomyces cerevisiae solid-state Anaerobic culturel: fermentation culture after the completion of step c with based on dregs of beans and wheat bran
Solid-state fermentation culture medium with the mixing of 3:7 weight ratio, be transferred to solid state fermentation case, solid state fermentation room temperature controls the fermentation at 30 DEG C
Anaerobic culturel 28 hours under the closed obstructed gaseity of case.
Step e, low temperature drying: the culture that step d is obtained is transferred in drying device, using 50 DEG C of hot winds by culture
Water content is down to 13% or less.
Step f, crushing and packaging: step e acquisition dry yeast culture is ground into 40 mesh granularities using pulverizer, is wrapped
Installation is packaged into bag, and Packing Unit is 10~50Kg/ bags, is protected from light storage in a cool and dry place.
The seed culture based component of saccharomyces cerevisiae is by weight percentage in step a are as follows: 0.5% yeast extract, 0.5% egg
White peptone, 1% bean cake powder, sucrose 2%, remaining is purified water.
The liquid state fermentation medium component of saccharomyces cerevisiae is by weight percentage in step b are as follows: 0.5% peptone, 1.5%
Bean cake powder, 1~1.5% corn flour, molasses 4%, remaining is purified water.
Solid-state fermentation culture medium ingredient is in step d with weight percent are as follows: 15% bean cake powder, 10% corn flour, 75% small
Wheat bran skin.
Dry yeast culture mannan content reaches 4%, and water-solubility protein reaches 7%.
Embodiment two
Step a, the preparation of saccharomyces cerevisiae seed liquor: 2 ring of saccharomyces cerevisiae strain of picking slant preservation is seeded to seed training
It supports in base, 32 DEG C, 250rpm shaking table shaken cultivation 16h；
Step b, saccharomyces cerevisiae liquid High Density Cultivation: seed culture fluid is seeded to equipped with the anti-of liquid state fermentation culture medium
It answers in tank, 32 DEG C of temperature, control pH are in 5.0~6.0 ranges in tank, speed of agitator 150rpm, Ventilation Rate 0.75vvm culture
18h。
Step c, high-content mannosan saccharomyces cerevisiae low temperature and hydrogen peroxide coercing cultivation: by the temperature of step b fermentation system
Degree is reduced to 26 DEG C, while being added to the hydrogen peroxide of 15% concentration at the uniform velocity stream in reactor tank by peristaltic pump, until final concentration reaches
8mmol/L continues culture 4 hours.
Step d, saccharomyces cerevisiae solid-state Anaerobic culturel: fermentation culture after the completion of step c with based on dregs of beans and wheat bran
Solid medium with the mixing of 3:7 weight ratio, be transferred to solid state fermentation case, solid state fermentation room temperature is controlled at 32 DEG C, and fermenting case is close
Close Anaerobic culturel 24 hours under obstructed gaseity.
Step e, low temperature drying: the culture that step d is obtained is transferred in drying device, using 70 DEG C of hot winds by culture
Water content is down to 13% or less.
Step f, crushing and packaging: step e acquisition dry yeast culture is ground into 60 mesh granularities using pulverizer, is wrapped
Installation is packaged into bag, and Packing Unit is 10~50Kg/ bags, is protected from light storage in a cool and dry place.
The seed culture based component of saccharomyces cerevisiae is compared in step a with weight percent are as follows: 1% yeast extract, 0.5% albumen
Peptone, 0.5% bean cake powder, sucrose 2%, remaining is purified water.
The liquid state fermentation medium component of saccharomyces cerevisiae is compared in step b with weight percent are as follows: 1% peptone, 2% dregs of beans
Powder, 1% corn flour, molasses 4%, remaining is purified water.
Solid-state fermentation culture medium ingredient is in step d with weight percent are as follows: 20% bean cake powder, 10% corn flour, 70% small
Wheat bran skin.
Dry yeast culture mannan content reaches 4.2%, and water-solubility protein reaches 9%.
Embodiment three
Step a, the preparation of saccharomyces cerevisiae seed liquor: 2 ring of saccharomyces cerevisiae strain of picking slant preservation is seeded to seed training
It supports in base, 31 DEG C, 230rpm shaking table shaken cultivation 17h；
Step b, saccharomyces cerevisiae liquid High Density Cultivation: seed culture fluid is seeded to equipped with the anti-of liquid state fermentation culture medium
It answers in tank, 5.5, speed of agitator 220rpm, Ventilation Rate 1.0vvm cultivate 19h by 31 DEG C of temperature, control pH in tank.
Step c, high-content mannosan saccharomyces cerevisiae low temperature and hydrogen peroxide coercing cultivation: reactor tank interior reaction temperature is dropped
The hydrogen peroxide of 20% concentration at the uniform velocity stream is added in reactor tank down to 25 DEG C, while by peristaltic pump, until hydrogen peroxide in reactor tank
Final concentration reach 7.5mmol/L, continue culture 5 hours.
Step d, saccharomyces cerevisiae solid-state Anaerobic culturel: fermentation culture after the completion of step c with based on dregs of beans and wheat bran
Solid medium with the mixing of 3:8 weight ratio, be transferred to solid state fermentation case, solid state fermentation room temperature is controlled at 31 DEG C, and fermenting case is close
Close Anaerobic culturel 26 hours under obstructed gaseity.
Step e, low temperature drying: the culture that step d is obtained is transferred in drying device, using 60 DEG C of hot winds by culture
Water content is down to 12% or less.
Step f, crushing and packaging: step e acquisition dry yeast culture is ground into 50 mesh granularities using pulverizer, is wrapped
Installation is packaged into bag, is protected from light storage in a cool and dry place.
The seed culture based component of saccharomyces cerevisiae is in step a with weight percent are as follows: 0.75% yeast extract, 0.1% egg
White peptone, 0.75% bean cake powder, sucrose 1%, remaining is purified water.
The liquid state fermentation medium component of saccharomyces cerevisiae is in step b with weight percent are as follows: 0.75% peptone, 1.75%
Bean cake powder, 1.25% corn flour, molasses 3%, remaining is purified water.
Solid-state fermentation culture medium ingredient is in step d with weight percent are as follows: 30% bean cake powder, 5% corn flour, 65% wheat
Wheat bran.
Dry yeast culture mannan content reaches 4.3%, and water-solubility protein reaches 8%.
Example one, embodiment two, dry yeast culture prepared by embodiment three respectively, according to control group and reality
The principle for testing group carries out feeding trial.Wherein control group and experimental group are using pairing experimental design, according to age, parity, figure
Principle similar in the body conditions such as size, feed intake, the output of milk and lactation cycle chooses lactation period Holstein cow 80, is divided into experiment
Group one, experimental group two, experimental group three and control group (group difference is not significant), every group 20 carry out feeding comparative experiments, experiment
Group one feeds dry yeast culture prepared by 300 grams of embodiments one daily, and experimental group two feeds 300 grams of two institutes of embodiment daily
Dry yeast culture is prepared, experimental group three feeds dry yeast culture prepared by 300 grams of embodiments three daily, and control group is not
Feeding yeast culture, remaining condition are identical.After feeding one month, output of milk comparison is carried out.
As shown in Table 1, each group average milk production is without significant difference before testing.Test later period, experimental group one, two and of experimental group
Three output of milk of experimental group is higher than 1.12~1.14kg/ of control group ﹒ days.Yeast culture causes feed intake to increase, to improve
Daily yielding.
1 output of milk of table compares
2 body cell of table compares
Body cell in milk not only can reflect the quality of cream, also directly reflect the health status etc. of cow breast,
As shown in table 2, body cell can be effectively reduced by feeding high-content mannosan yeast culture, improves milk cow body
Immunity, mammitis case incidence are remarkably decreased, and effectively reduce pasture because mammitis and antibiotic usage bring are negative
It influences and loses.
It can be seen that improvement master the most apparent by above-mentioned milk cow high-content mannosan yeast culture administering transgenic
It embodies both ways: first, lactation amount dramatically increases, and every daily average milk production of cow head increases by 1.14 kilograms, and (1~2 is public
Jin), remarkable in economical benefits；Second, body cell is remarkably decreased, and mammitis case incidence significantly reduces, and effectively reduces pasture
Because mammitis and the negative effect of antibiotic usage bring and loss, milk matter are promoted, it is conducive to drinking person health.
Claims (2)
1. a kind of production method of high-content mannosan yeast culture, it is characterised in that the following steps are included:
Step a, the preparation of saccharomyces cerevisiae seed liquor: 1 ~ 2 ring of saccharomyces cerevisiae strain of picking slant preservation is seeded to seed culture
In base, in shaking table shaken cultivation, saccharomyces cerevisiae seed liquor is obtained；
Step b, saccharomyces cerevisiae liquid High Density Cultivation: saccharomyces cerevisiae seed liquor is seeded to equipped with the anti-of liquid state fermentation culture medium
It answers Yu Zhongwen in tank, cultivated under weakly acidic condition；
Step c, high-content mannosan saccharomyces cerevisiae low temperature and hydrogen peroxide coercing cultivation: the reaction temperature of reactor tank is reduced, together
When the hydrogen peroxide of 15% ~ 25% mass concentration at the uniform velocity stream is added in reactor tank, until in reactor tank the final concentration of hydrogen peroxide reach 6 ~
10mmol/L then proceedes to cultivate several hours, obtains fermentation culture；
Step d, saccharomyces cerevisiae solid-state Anaerobic culturel: fermentation culture is mixed with solid medium according to certain weight ratio, is obtained
To mixture, mixture is then transferred to solid state fermentation case Anaerobic culturel under closed obstructed gaseity, obtains culture；
Step e, low temperature drying: culture is transferred in drying device, is dried culture using low temperature hot wind, is obtained
Dry yeast culture；
Step f, crushing and packaging: dry yeast culture being crushed, is packaged into bag, is protected from light storage in a cool and dry place；
Saccharomyces cerevisiae strain is CICC1355, the seed culture medium each component and its weight percent of saccharomyces cerevisiae strain in step a
Than are as follows: 0.5 ~ 1% yeast extract, 0.5 ~ 1% peptone, 0.5 ~ 1% bean cake powder, sucrose 1~2%, remaining is purified water；
The temperature of shaking table shaken cultivation is 30 ~ 32 DEG C in step a, oscillation revolving speed is 200 ~ 250 rpm, the shaken cultivation time is
16 ~ 18h；
The liquid state fermentation culture medium each component and its weight percent of saccharomyces cerevisiae in the step b are as follows: 0.5~1% peptone,
1.5~2% bean cake powders, 1~1.5% corn flour, molasses 2~4%, remaining is purified water；
In step b reactor tank interior reaction temperature be 30 ~ 32 DEG C, pH be 5.0 ~ 6.0, speed of agitator be 150 ~ 250 rpm, ventilation speed
Rate is 0.5 ~ 1.0vvm, incubation time is 18 ~ 20h；
Reactor tank interior reaction temperature is reduced to 24 ~ 26 DEG C in step c, while being added to hydrogen peroxide at the uniform velocity stream instead by peristaltic pump
It answers in tank；Continuing incubation time is 4~6 hours；
In step d the Mixing ratio by weight of fermentation culture and solid medium be 3:6~3:8, each component of solid medium and
Its weight percent are as follows: 15~35% bean cake powders, 5~10% corn flour, 55~80% wheat brans；
The solid state fermentation case Anaerobic culturel time is 24~28 hours in step d, and the temperature of solid state fermentation room is in solid state fermentation case
30~32℃；
The temperature of low temperature hot wind is 50 DEG C ~ 70 DEG C in step e, and the water content of dry yeast culture is 13% hereinafter, dry yeast
Culture mannan content reaches 4 ~ 4.5%, and water-solubility protein reaches 7%~9%.
2. the production method of high-content mannosan yeast culture according to claim 1, it is characterised in that: step f
Middle that dry yeast culture is ground into 40~60 mesh granularities using pulverizer, packing machine is packaged into bag, Packing Unit 10
~50Kg/ bags.
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