CN106520585A - Production method for high-content mannan yeast culture - Google Patents
Production method for high-content mannan yeast culture Download PDFInfo
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Abstract
The invention discloses a production method for a high-content mannan yeast culture and belongs to the technical field of bioengineering. The production method comprises the steps that a wine making yeast seed solution is inoculated into a liquid fermentation culture medium at first, and aerobic culturing is conducted for 18-20 h at the temperature of 30 DEG C-32 DEG C, so that a thallus reaches the high-density level; then the culturing temperature is lowered to 24 DEG C-26 DEG C, hydrogen peroxide is added into a fermentation system at the same time till the final concentration is 6-10 mmol/L, and culturing is continuously conducted; and a fermentation culture solution and a solid culture medium are mixed at the weight ratio of 3:6-3:8 and are transferred into a solid fermentation box to be subjected to anaerobic culturing for 24-28 hours, then low-temperature drying is conducted, and the high-content mannan yeast culture can be obtained through smashing. The mannan content of the high-content mannan yeast culture is 4.0%-4.5%, and the water-soluble protein is 7%-9%. According to the high-content mannan yeast culture, the cow milk yield can be significantly increased by 1-2 kg/(head.day), the number of cow somatic cells is effectively decreased, the cow health condition is significantly improved, and the high-content mannan yeast culture is good for the drinker health.
Description
Technical field
The present invention relates to a kind of production method of high-load mannan yeast culture, belongs to biotechnology neck
Domain.
Background technology
Yeast culture typically refers to what saccharomyces cerevisiae was obtained through fully fermentation under special process and nutritional condition
Compound criteria thing, the culture are mainly made up of three parts：Yeast thalline, yeast metabolism product, the nutrient matrix of degeneration, these
Rich in effective ingredient and the part unknown function factors such as mannan, glucosan, aminoacid, vitamin B group, nucleic acid in component.
Used as functional fodder raw material, yeast culture has functions that significant disease-resistant, volume increase, can be to micro- life in animal alimentary canal
Thing provides nutrient substance, so as to exciting their metabolic activity and stablizing internal microecological environment, with antibioticses additive phase
Than there is green natural, having no toxic side effect and good palatability, nutritious, therefore be widely used in poultry and support
Grow field.
The function and its mechanism of yeast culture is mainly：Improve gastrointestinal bacterial flora to fall structure, improve breeding performonce fo animals；
Supplement the nutrients, improve digestive function, improve immunity of organisms and premunition.
Mannan is the key function factor of yeast culture, and it can dramatically increase animal body fluid immunity and cellular immunization
Ability, regulating intestinal canal colony balance with reference to the exogenous pathogen of absorption, selective absorption bacteriotoxin, and have radioprotective, resist
Oxidation, antitumor isoreactivity function.In consideration of it, the Ministry of Agriculture of on December 19th, 2013 issues No. 2038 bulletin, by " saccharomyces cerevisiae
Culture " from《Catalogue of feed additive varieties》Proceed to《Feedstuff catalogue》, and mannan is mandatory as the product
Mark requires index, and mannan has some idea of to the importance of Cultures of S. cerevisiae.
Mannan is located on the outside of yeast cell wall, and yeast cell wall is typical sandwich structure, intermediate course be by
Protein is constituted, and both sides are caused by mannan and beta glucan and fraction chitin, phospholipid and fiber substance respectively
Close to conclude into three-dimensional space net structure, yeast mannans account for the 10-20% of yeast dry weight.The content of yeast mannans
Found mannan content by Yeast Cultivation trophic factor system under study for action by the appreciable impact of external environment condition, Liu Hongzhi etc.
About；Ni Jingyue etc. has found the content of condition of culture appreciable impact mannan under study for action.
Although mannan is the key function factor of yeast culture, yeast culture production technology pertinent literature
In be but rarely reported, the technical research of current yeast culture is focused primarily upon in the selection of cheap substrates, after all mainly
It is for reduces cost, turns waste into wealth, such as Chinese patent (application number：201410076270.0) report with yellow wine lees as substrate
Carry out the preparation of yeast culture；And Chinese patent (application number：201510319359.X) report by substrate of distillers ' grains
The mobile fermentation of row hygrometric state yeast culture；Additionally, Chinese patent (application number：201510578518.8) report to mix grain
Slag carries out the fermenting and producing of yeast culture for raw material.
Saccharomyces cerevisiae is amphimicrobe, is suitable for various culture environments, but its substrate is converted and product generation in different environments
The approach of thanking is not quite similar, in order that yeast culture is through fully fermentation, sweat generally merges liquid-solid fermentation, good
Various training modes such as oxygen-anaerobic fermentation.How in the sweat of various training modes fusion, by modulation process parameter
To improve mannan content for the production technology innovation of yeast culture has important practical significance.
The content of the invention
The technical problem to be solved in the invention is to provide a kind of production method of high-load mannan yeast culture,
4.0～4.5% are reached using mannan content in the yeast culture of the technique productions, water-solubility protein reaches 7%～
9%.
To solve above-mentioned technical problem, the technical solution adopted in the present invention is：
A kind of production method of high-load mannan yeast culture, comprises the following steps：
The preparation of step a, saccharomyces cerevisiae seed liquor：1～2 ring of saccharomyces cerevisiae strain of picking slant preservation, is seeded to kind
In sub- culture medium, in shaking table shaken cultivation, saccharomyces cerevisiae seed liquor is obtained；
Step b, saccharomyces cerevisiae liquid High Density Cultivation：Saccharomyces cerevisiae seed liquor is seeded to equipped with liquid fermentation culture medium
Retort under middle temperature, weakly acidic condition cultivate；
Step c, high-load mannan saccharomyces cerevisiae low temperature and hydrogen peroxide coercing cultivation：Reduce the reaction temperature of retort
Degree, while the hydrogen peroxide of 15%～25% mass concentration at the uniform velocity stream is added in retort, to retort, the end of hydrogen peroxide is dense
Degree reaches 6～10mmol/L, then proceedes to cultivate some hours, obtains fermentation culture；
Step d, saccharomyces cerevisiae solid-state Anaerobic culturel：Fermentation culture is mixed according to certain weight ratio with solid medium
Close, obtain mixture, mixture is transferred to into solid fermentation case Anaerobic culturel under closed obstructed gaseity then, is cultivated
Thing；
Step e, oven drying at low temperature：Culture is transferred in drying device, culture is dried using low temperature hot blast,
Obtain dry yeast culture；
Step f, crushing and packaging：Dry yeast culture is crushed, bag is packaged into, lucifuge is deposited at shady and cool drying.
Technical solution of the present invention further improvement is that：In step a saccharomyces cerevisiae strain be CICC1355, saccharomyces cerevisiae
The seed culture medium each component of strain and its percentage by weight are：0.5～1% yeast extract, 0.5～1% peptone, 0.5～
1% bean cake powder, sucrose 1～2%, remaining is purified water.
Technical solution of the present invention further improvement is that：In step a, the temperature of shaking table shaken cultivation is 30～32 DEG C, shakes
It is 16～18h to swing rotating speed for 200～250rpm, shaken cultivation time.
Technical solution of the present invention further improvement is that：In step b, the liquid fermentation culture medium of saccharomyces cerevisiae is each
Component and its percentage by weight are：0.5～1% peptone, 1.5～2% bean cake powders, 1～1.5% Semen Maydis powder, molasses 2～4%,
Remaining is purified water.
Technical solution of the present invention further improvement is that：In step b retort interior reaction temperature be 30～32 DEG C, pH be
5.0～6.0, speed of agitator be 150～250rpm, Ventilation Rate be 0.5～1.0vvm, incubation time be 18～20h.
Technical solution of the present invention further improvement is that：Retort interior reaction temperature is reduced to into 24～26 in step c
DEG C, while hydrogen peroxide at the uniform velocity stream is added in retort by peristaltic pump；It is 4～6 hours to continue incubation time.
Technical solution of the present invention further improvement is that：In step d, fermentation culture is mixed with the weight of solid medium
Composition and division in a proportion is 3:6～3:8, each component and its percentage by weight of solid medium are：15～35% bean cake powders, 5～10% Semen Maydiss
Powder, 55～80% Testa Tritici.
Technical solution of the present invention further improvement is that：In step d, the solid fermentation case Anaerobic culturel time is 24～28
Hour, in solid fermentation case, the temperature of solid fermentation room is 30～32 DEG C.
Technical solution of the present invention further improvement is that：In step e, the temperature of low temperature hot blast is 50 DEG C～70 DEG C, is dried
The water content of yeast culture is less than 13%, and dry yeast culture mannan content reaches 4～4.5%, water solublity egg
7%～9% is reached in vain.
Technical solution of the present invention further improvement is that：Used in step f, dry yeast culture is crushed by pulverizer
Into 40～60 mesh granularities, packer is packaged into bag, and Packing Unit is 10～50Kg/ bags.
As a result of above-mentioned technical proposal, the technological progress that the present invention is obtained is：
It is an object of the invention to provide a kind of production method of high-load mannan yeast culture.Whole production process
Employ liquid it is aerobic-mode cultivated stage by stage of solid-state anaerobism；The low temperature that adopts in the later stage in liquid aerobic fermentation stage and double
Oxygen water oxygen coercing cultivation is conducive to the formation of high-load mannan saccharomyces cerevisiae, using the yeast culture of the technique productions
Middle mannan content reaches 4.0～4.5%, and water-solubility protein reaches 7%～9%.CICC1355 therein is bought in China
Research for Industrial Microbial Germ preservation administrative center, belongs to《Chinese microorganism strain catalogue》In Research for Industrial Microbial Germ, bacterium number
1355 Chineses of CICC：Saccharomyces cerevisiae, plants name：Saccharomyces, generic name cerevisiae, purposes：Feed yeast.
The principle of the present invention：First, the aerobic high density fermentation mode of liquid that early stage is adopted provides foot for follow-up phase
The microbial cell of enough amounts；Then the low temperature and hydrogen peroxide oxidation coercing cultivation for adopting is conducive to high-load mannan to make wine
The formation of yeast；The solid anaerobic digestion of final stage be conducive to yeast fully ferment synthesis more horn of plenty functional components.
After fermentation ends, solid state fermentation culture thing is carried out cold drying, crushed, that is, obtains finished product.
In step a, the seed culture medium each component and its percentage by weight of saccharomyces cerevisiae strain is：0.5～1% yeast soaks
Cream, 0.5～1% peptone, 0.5～1% bean cake powder, sucrose 1～2%, remaining is purified water.Configuration is simple, is of high nutritive value；
In step b, the liquid fermentation culture medium each component and its percentage by weight of saccharomyces cerevisiae is：0.5～1% peptone, 1.5～2%
Bean cake powder, 1～1.5% Semen Maydis powder, molasses 2～4%, remaining is purified water, and configuration is simple, nutritious, wherein the sugar selected
Honey is a kind of sticky, pitchy, in semifluid object, mainly contain sucrose, in cane molasses, pantothenic acid content is higher, up to 37mg/
Kg, biological cellulose content is also very considerable in addition.Molasses are the side-products of sugar industry, are constituted because refining sugar raw material, processing conditionss not
It is variant together, wherein mainly containing a large amount of fermentable sugars (mainly sucrose), thus it is good fermentation raw material, can be used as ferment
The substrate or base material of the fermented products such as mother, monosodium glutamate, organic acid, can be used as the raw material and animal feed of some food.
Low temperature and hydrogen peroxide oxidation coercing cultivation are conducive to the formation of high-load mannan saccharomyces cerevisiae, and liquid is aerobic-
The mode of solid-state anaerobism multi-mode culture stage by stage is conducive to yeast fully to ferment the functional components of synthesis more horn of plenty.This
Bright technology stability is good, without mould contamination, product safety；It is of high nutritive value；Process is simple, low cost, easily operated, quality
Stable, production process environmental protection, non-environmental-pollution, parameter are easily controllable, and fermentation efficiency and fermented quality are high.
Specific embodiment
A kind of production method of high-load mannan yeast culture, comprises the following steps：
The preparation of step a, saccharomyces cerevisiae seed liquor：1～2 ring of saccharomyces cerevisiae strain of picking slant preservation, is seeded to kind
In sub- culture medium, in shaking table shaken cultivation, saccharomyces cerevisiae seed liquor is obtained；
Step b, saccharomyces cerevisiae liquid High Density Cultivation：Saccharomyces cerevisiae seed liquor is seeded to equipped with liquid fermentation culture medium
Retort under middle temperature, weakly acidic condition cultivate；
Step c, high-load mannan saccharomyces cerevisiae low temperature and hydrogen peroxide coercing cultivation：Reduce the reaction temperature of retort
Degree, while the hydrogen peroxide of 15%～25% mass concentration at the uniform velocity stream is added in retort, to retort, the end of hydrogen peroxide is dense
Degree reaches 6～10mmol/L, then proceedes to cultivate some hours, obtains fermentation culture；
Step d, saccharomyces cerevisiae solid-state Anaerobic culturel：Fermentation culture is mixed according to certain weight ratio with solid medium
Close, obtain mixture, mixture is transferred to into solid fermentation case Anaerobic culturel under closed obstructed gaseity then, is cultivated
Thing；
Step e, oven drying at low temperature：Culture is transferred in drying device, culture is dried using low temperature hot blast,
Obtain dry yeast culture；
Step f, crushing and packaging：Dry yeast culture is crushed, bag is packaged into, lucifuge is deposited at shady and cool drying.
In step a, saccharomyces cerevisiae strain is CICC1355, the seed culture medium each component of saccharomyces cerevisiae strain and its weight
Percentage ratio is：0.5～1% yeast extract, 0.5～1% peptone, 0.5～1% bean cake powder, sucrose 1～2%, remaining is purification
Water.
In step a the temperature of shaking table shaken cultivation be 30～32 DEG C, vibration rotating speed for 200～250rpm, shaken cultivation when
Between be 16～18h.
In step b, the liquid fermentation culture medium each component and its percentage by weight of saccharomyces cerevisiae is：0.5～1% egg
White peptone, 1.5～2% bean cake powders, 1～1.5% Semen Maydis powder, molasses 2～4%, remaining is purified water.
In step b retort interior reaction temperature be 30～32 DEG C, pH be 5.0～6.0, speed of agitator be 150～250rpm,
Ventilation Rate is 0.5～1.0vvm, incubation time is 18～20h.
Retort interior reaction temperature is reduced to into 24～26 DEG C in step c, while at the uniform velocity flowing hydrogen peroxide by peristaltic pump
Add in retort；It is 4～6 hours to continue incubation time.
In step d, fermentation culture and the Mixing ratio by weight of solid medium are 3:6～3:8, each group of solid medium
Part and its percentage by weight are：15～35% bean cake powders, 5～10% Semen Maydis powder, 55～80% Testa Tritici.
In step d, the solid fermentation case Anaerobic culturel time is 24～28 hours, the temperature of solid fermentation room in solid fermentation case
Spend for 30～32 DEG C.
In step e, the temperature of low temperature hot blast is 50 DEG C～70 DEG C, and the water content of dry yeast culture is less than 13%, is done
Dry yeast culture mannan content reaches 4～4.5%, and water-solubility protein reaches 7%～9%.
Used in step f, dry yeast culture is ground into 40～60 mesh granularities by pulverizer, and packer is packaged into
Bag, Packing Unit are 10～50Kg/ bags.
The present invention is described in further details with reference to embodiment：
Embodiment one：
A kind of production method of high-load mannan yeast culture, comprises the following steps：
The preparation of step a, yeast starter liquid：2 ring of saccharomyces cerevisiae (CICC1355) strain of picking slant preservation, is seeded to
In seed culture medium, 30 DEG C, 200rpm shaking table shaken cultivation 18h.
Step b, saccharomyces cerevisiae liquid High Density Cultivation：Seed culture fluid is seeded to equipped with liquid fermentation culture medium
In 1000 liters of retort, 30 DEG C of temperature in tank, control pH in the range of 5.0～6.0, speed of agitator 200rpm, Ventilation Rate
1.0vvm cultivates 20h.
Step c, high-load mannan saccharomyces cerevisiae low temperature and hydrogen peroxide coercing cultivation：By the temperature of step b fermentation system
Degree is reduced to 24 DEG C, while the hydrogen peroxide of 20% mass concentration at the uniform velocity stream is added in retort by peristaltic pump, to hydrogen peroxide
Final concentration reaches 8mmol/L, continues culture 6 hours.
Step d, saccharomyces cerevisiae solid-state Anaerobic culturel：Fermentation culture after the completion of step c with based on bean cake and wheat bran
Solid-state fermentation culture medium with 3:7 weight are transferred to solid fermentation case than mixing, and solid fermentation room temperature is controlled at 30 DEG C, fermentation
Anaerobic culturel 28 hours under the closed obstructed gaseity of case.
Step e, oven drying at low temperature：The culture that step d is obtained is transferred in drying device, using 50 DEG C of hot blasts by culture
Water content is down to less than 13%.
Step f, crushing and packaging：Step e acquisition dry yeast culture is ground into into 40 mesh granularities using pulverizer, is wrapped
Installation is packaged into bag, and Packing Unit is 10～50Kg/ bags, and lucifuge is deposited at shady and cool drying.
In step a, the seed culture based component of saccharomyces cerevisiae is by weight percentage：0.5% yeast extract, 0.5% egg
White peptone, 1% bean cake powder, sucrose 2%, remaining is purified water.
In step b, the liquid fermentation medium component of saccharomyces cerevisiae is by weight percentage：0.5% peptone, 1.5%
Bean cake powder, 1～1.5% Semen Maydis powder, molasses 4%, remaining is purified water.
In step d, solid-state fermentation culture medium composition with percentage by weight is：It is 15% bean cake powder, 10% Semen Maydis powder, 75% little
Testa Tritici skin.
Dry yeast culture mannan content reaches 4%, and water-solubility protein reaches 7%.
Embodiment two
The preparation of step a, saccharomyces cerevisiae seed liquor：2 ring of saccharomyces cerevisiae strain of picking slant preservation, is seeded to seed training
In foster base, 32 DEG C, 250rpm shaking table shaken cultivation 16h；
Step b, saccharomyces cerevisiae liquid High Density Cultivation：Seed culture fluid is seeded to equipped with the anti-of liquid fermentation culture medium
In answering tank, 32 DEG C of temperature in tank, control pH in the range of 5.0～6.0, speed of agitator 150rpm, Ventilation Rate 0.75vvm culture
18h。
Step c, high-load mannan saccharomyces cerevisiae low temperature and hydrogen peroxide coercing cultivation：By the temperature of step b fermentation system
Degree is reduced to 26 DEG C, while the hydrogen peroxide of 15% concentration at the uniform velocity stream is added in retort by peristaltic pump, reaches to final concentration
8mmol/L, continues culture 4 hours.
Step d, saccharomyces cerevisiae solid-state Anaerobic culturel：Fermentation culture after the completion of step c with based on bean cake and wheat bran
Solid medium with 3:7 weight are transferred to solid fermentation case than mixing, and solid fermentation room temperature is controlled at 32 DEG C, and fermenting case is close
Close Anaerobic culturel 24 hours under obstructed gaseity.
Step e, oven drying at low temperature：The culture that step d is obtained is transferred in drying device, using 70 DEG C of hot blasts by culture
Water content is down to less than 13%.
Step f, crushing and packaging：Step e acquisition dry yeast culture is ground into into 60 mesh granularities using pulverizer, is wrapped
Installation is packaged into bag, and Packing Unit is 10～50Kg/ bags, and lucifuge is deposited at shady and cool drying.
In step a, the seed culture based component of saccharomyces cerevisiae with weight percent is frequently：1% yeast extract, 0.5% albumen
Peptone, 0.5% bean cake powder, sucrose 2%, remaining is purified water.
In step b, the liquid fermentation medium component of saccharomyces cerevisiae with weight percent is frequently：1% peptone, 2% bean cake
Powder, 1% Semen Maydis powder, molasses 4%, remaining is purified water.
In step d, solid-state fermentation culture medium composition with percentage by weight is：It is 20% bean cake powder, 10% Semen Maydis powder, 70% little
Testa Tritici skin.
Dry yeast culture mannan content reaches 4.2%, and water-solubility protein reaches 9%.
Embodiment three
The preparation of step a, saccharomyces cerevisiae seed liquor：2 ring of saccharomyces cerevisiae strain of picking slant preservation, is seeded to seed training
In foster base, 31 DEG C, 230rpm shaking table shaken cultivation 17h；
Step b, saccharomyces cerevisiae liquid High Density Cultivation：Seed culture fluid is seeded to equipped with the anti-of liquid fermentation culture medium
In answering tank, in tank, 5.5, speed of agitator 220rpm, Ventilation Rate 1.0vvm cultivate 19h for 31 DEG C of temperature, control pH.
Step c, high-load mannan saccharomyces cerevisiae low temperature and hydrogen peroxide coercing cultivation：Retort interior reaction temperature is dropped
As little as 25 DEG C, while the hydrogen peroxide of 20% concentration at the uniform velocity stream is added in retort by peristaltic pump, the hydrogen peroxide to retort
Final concentration reach 7.5mmol/L, continue culture 5 hours.
Step d, saccharomyces cerevisiae solid-state Anaerobic culturel：Fermentation culture after the completion of step c with based on bean cake and wheat bran
Solid medium with 3:8 weight are transferred to solid fermentation case than mixing, and solid fermentation room temperature is controlled at 31 DEG C, and fermenting case is close
Close Anaerobic culturel 26 hours under obstructed gaseity.
Step e, oven drying at low temperature：The culture that step d is obtained is transferred in drying device, using 60 DEG C of hot blasts by culture
Water content is down to less than 12%.
Step f, crushing and packaging：Step e acquisition dry yeast culture is ground into into 50 mesh granularities using pulverizer, is wrapped
Installation is packaged into bag, and lucifuge is deposited at shady and cool drying.
In step a, the seed culture based component of saccharomyces cerevisiae with percentage by weight is：0.75% yeast extract, 0.1% egg
White peptone, 0.75% bean cake powder, sucrose 1%, remaining is purified water.
In step b, the liquid fermentation medium component of saccharomyces cerevisiae with percentage by weight is：0.75% peptone, 1.75%
Bean cake powder, 1.25% Semen Maydis powder, molasses 3%, remaining is purified water.
In step d, solid-state fermentation culture medium composition with percentage by weight is：30% bean cake powder, 5% Semen Maydis powder, 65% Semen Tritici aestivi
Wheat bran.
Dry yeast culture mannan content reaches 4.3%, and water-solubility protein reaches 8%.
Dry yeast culture prepared by difference Example one, embodiment two, embodiment three, according to matched group and reality
The principle for testing group carries out feeding trial.Wherein matched group and experimental group adopt pairing experimental design, according to age, parity, build
The close principle of the body conditions such as size, feed intake, milk yield and lactation cycle, chooses lactation period Holstein cow 80, is divided into experiment
Group one, experimental group two, experimental group three and matched group (group difference is not notable), 20 per group carry out feeding contrast experiment, experiment
Group one feeds dry yeast culture prepared by 300 grams of embodiments one daily, and experimental group two feeds 300 grams of two institutes of embodiment daily
Dry yeast culture is prepared, experimental group three feeds dry yeast culture prepared by 300 grams of embodiments three daily, and matched group is not
Feeding yeast culture, remaining condition are identical.After feeding one month, milk yield comparison is carried out.
As shown in Table 1, before testing, each group average milk production is without significant difference.Experiment later stage, experimental group one, two and of experimental group
Three milk yield of experimental group is higher than matched group 1.12～1.14kg/ previous day.Yeast culture causes feed intake to increase, so as to improve
Daily yielding.
1 milk yield of table compares
2 somatic cell of table is compared
Somatic cell in milk can not only reflect the quality of breast, also directly reflect health status of cow breast etc.,
As shown in table 2, somatic cell can be effectively reduced by feeding high-load mannan yeast culture, improve milch cow body
Immunity, mastitiss case incidence rate are remarkably decreased, effectively reduce pasture because mastitiss and antibiotic usage bring it is negative
Affect and lose.
The most obvious improvement be can be seen that by above-mentioned milch cow high-load mannan yeast culture administering transgenic to lead
Embody both ways：First, lactation amount is dramatically increased, and per cow head, (1～2 is public for 1.14 kilograms of daily average milk production increase
Jin), remarkable in economical benefits；Second, somatic cell is remarkably decreased, and mastitiss case incidence rate is significantly reduced, and effectively reduces pasture
The negative effect brought because of mastitiss and antibiotic usage and loss, milk matter are lifted, beneficial to drinking person health.
Claims (10)
1. a kind of production method of high-load mannan yeast culture, it is characterised in that comprise the following steps：
The preparation of step a, saccharomyces cerevisiae seed liquor：1～2 ring of saccharomyces cerevisiae strain of picking slant preservation, is seeded to seed training
In foster base, in shaking table shaken cultivation, saccharomyces cerevisiae seed liquor is obtained；
Step b, saccharomyces cerevisiae liquid High Density Cultivation：Saccharomyces cerevisiae seed liquor is seeded to equipped with the anti-of liquid fermentation culture medium
Cultivate under middle temperature, weakly acidic condition in answering tank；
Step c, high-load mannan saccharomyces cerevisiae low temperature and hydrogen peroxide coercing cultivation：The reaction temperature of retort is reduced, together
When the hydrogen peroxide of 15%～25% mass concentration at the uniform velocity stream is added in retort, to retort, the final concentration of hydrogen peroxide reaches
6～10mmol/L, then proceedes to cultivate some hours, obtains fermentation culture；
Step d, saccharomyces cerevisiae solid-state Anaerobic culturel：Fermentation culture is mixed according to certain weight ratio with solid medium, is obtained
To mixture, mixture is transferred to into solid fermentation case Anaerobic culturel under closed obstructed gaseity then, culture is obtained；
Step e, oven drying at low temperature：Culture is transferred in drying device, culture is dried using low temperature hot blast, is obtained
Dry yeast culture；
Step f, crushing and packaging：Dry yeast culture is crushed, bag is packaged into, lucifuge is deposited at shady and cool drying.
2. the production method of high-load mannan yeast culture according to claim 1, it is characterised in that：Step a
Middle saccharomyces cerevisiae strain is CICC1355, and the seed culture medium each component of saccharomyces cerevisiae strain and its percentage by weight are：0.5～
1% yeast extract, 0.5～1% peptone, 0.5～1% bean cake powder, sucrose 1～2%, remaining is purified water.
3. the production method of high-load mannan yeast culture according to claim 1, it is characterised in that：Step a
The temperature of middle shaking table shaken cultivation is 30～32 DEG C, vibration rotating speed is 200～250rpm, the shaken cultivation time is 16～18h.
4. the production method of high-load mannan yeast culture according to claim 1, it is characterised in that：The step
In rapid b, the liquid fermentation culture medium each component and its percentage by weight of saccharomyces cerevisiae is：0.5～1% peptone, 1.5～2% beans
Dregs of rice powder, 1～1.5% Semen Maydis powder, molasses 2～4%, remaining is purified water.
5. the production method of high-load mannan yeast culture according to claim 1, it is characterised in that：Step b
Middle retort interior reaction temperature is 30～32 DEG C, pH is 5.0～6.0, speed of agitator is 150～250rpm, Ventilation Rate is 0.5
～1.0vvm, incubation time are 18～20h.
6. the production method of high-load mannan yeast culture according to claim 1, it is characterised in that：Step c
It is middle that retort interior reaction temperature is reduced to into 24～26 DEG C, while hydrogen peroxide at the uniform velocity stream is added in retort by peristaltic pump；
It is 4～6 hours to continue incubation time.
7. the production method of high-load mannan yeast culture according to claim 1, it is characterised in that：Step d
Middle fermentation culture is 3 with the Mixing ratio by weight of solid medium:6～3:8, each component and its weight percent of solid medium
Than for：15～35% bean cake powders, 5～10% Semen Maydis powder, 55～80% Testa Tritici.
8. the production method of high-load mannan yeast culture according to claim 1, it is characterised in that：Step d
The middle solid fermentation case Anaerobic culturel time is 24～28 hours, and in solid fermentation case, the temperature of solid fermentation room is 30～32 DEG C.
9. the production method of high-load mannan yeast culture according to claim 1, it is characterised in that：Step e
The temperature of middle low temperature hot blast is 50 DEG C～70 DEG C, and the water content of dry yeast culture is less than 13%, dry yeast culture
Mannan content reaches 4～4.5%, and water-solubility protein reaches 7%～9%.
10. the production method of high-load mannan yeast culture according to claim 1, it is characterised in that：Step f
Used in pulverizer dry yeast culture is ground into into 40～60 mesh granularities, packer is packaged into bag, and Packing Unit is 10
～50Kg/ bags.
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| CN115005327B (en) * | 2022-06-13 | 2023-08-11 | 河北斐默特生物科技有限公司 | Fermentation type feeding carrier and preparation method thereof |
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