CN106497830A - The method that compound microorganism ferments prepare Sargassum granular potash fertilizer - Google Patents
The method that compound microorganism ferments prepare Sargassum granular potash fertilizer Download PDFInfo
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- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
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Abstract
The present invention relates to a kind of method that compound microorganism ferments prepare Sargassum granular potash fertilizer.First by clean for fresh marine algae plant, remove impurity, immersion, defibrination, then the seed liquor of strain is prepared respectively, seed liquor is inoculated into several times in Sargassum bed material, realize staged fermentation, tunning is further prepared into Sargassum function microbial inoculum, then which is combined with chemical fertilizer raw material by gentle reaction type prilling, finally gives the Algae synergistic potassium fertilizer particle rich in Sargassum extract, specially good effect composite bacteria.Experiment shows that the composite ratings fermentation technique effect that the present invention is adopted is fermented better than single culture, and the fertilizer efficiency of Sargassum functional type potash fertilizer prepared by the present invention is apparently higher than traditional chemical potash fertilizer products.
Description
Technical field
The invention belongs to agricultural fertilizer field, and in particular to a kind of compound microorganism ferments prepare the side of Sargassum granular potash fertilizer
Method.
Background technology
The excessive administration of chemical fertilizer causes Soil structure destruction, nature biotechnology multiformity and ecosystem stability drop
A series of more serious problems such as the eutrophication of low, rivers and lakes, agricultural product quality decline.Potash fertilizer product in the market
It is the compound of chemistry filler to plant mostly, lacks feature synergistic component, and the compound fertilizer and Blending Fertilizer prepared with this raw material can not be
Soil environment improves and the sustainable development of agricultural brings any benifit, can form vicious cycle on the contrary.Developing modern agriculture,
Realize agricultural sustainable development, new higher requirement will be proposed to the development of fertilizer industry, this require traditional into
Single chemical fertilizer is divided to upgrade to feature enhanced fertilizer.It is demonstrated experimentally that increasing in soil, beneficial microorganism content can be with
Play balanced soil acid-base value, adjust soil Bacterial community, the ability fertilizer of the disease and insect resistance of enhancing crop, a degraded soil huge sum of money
Category, the effect of the amount of changing soil.The secretion of endogenous hormones in plant body can be promoted in Sargassum containing active component, stimulate plant non-
The generation of the activity specific factor, also plants mineral element and rich containing non-nitrogen containing Organic substance, potassium, calcium, magnesium, zinc, iodine etc. more than ten
Rich vitamin, can effectively facilitate the growth of plant, and upgrading is increased production.
The research and development pattern of China's kind fertilizer is still more traditional, and apparatus and process is not enough, does not still become function
Property enhanced fertilizer Innovation Main Body, there is also some problems and not enough：1) strain is immature with chemical fertilizer complex process, destroys bacterium
Body, cause add bacterium amount many, survival rate is low；2) traditional microbial manure adopts a large amount of organic matters for filler, causes in storage
During miscellaneous bacteria amount reproduction, miscellaneous bacteria rate is higher, and microorganism-decomposing can thoroughly not cause rotten of burn seedlings；3) microorganism is deposited in fertilizer
During putting, bacterium amount declines rapidly, and the shelf-life is short, and living bacteria count is low；4) used by, strain vitality is not strong, it is difficult in soil
Original microorganism is resisted and gradually dead failure；5) to the immature destruction for causing active substance of the handling process of Sargassum and stream
Lose.
Content of the invention
The technical problem to be solved is to provide the side that a kind of compound microorganism ferments prepare Sargassum granular potash fertilizer
Method.Sargassum is carried out being classified biofermentation preparation Sargassum function microbial inoculum by multiple specially good effect strains, then by gentle counterattack
Which is combined by formula prilling with chemical fertilizer raw material, finally gives the Algae synergistic rich in Sargassum extract, specially good effect composite bacteria
Potassium fertilizer particle.Stable processing technique is controllable, environmental protection, is suitable to large-scale production.
In order to solve above-mentioned technical problem, the present invention is employed the following technical solutions：
A kind of method that compound microorganism ferments prepare Sargassum granular potash fertilizer, it is characterised in that comprise the following steps：
1) product A is prepared：By clean for fresh marine algae plant, remove impurity, after immersion 12-48h, defibrination proceeds to fermentation
In tank, 110-150 DEG C of sterilizing 15-30min is cooled to 20-35 DEG C and obtains product A；
2) seed liquor is prepared：Bacillus cereuss are activated and is spread cultivation, prepare bacillus cereuss seed liquor X；Will be huge
Bacillus cereuss are activated and are spread cultivation, and prepare bacillus megaterium seed liquor Y；Bacillus amyloliquefaciens are activated and is spread cultivation, prepare solution
Bacillus amyloliquefacienses seed liquor Z；
3) product B is prepared：Seed liquor X is inoculated in product A, 10%-20% of the seed liquor X consumption for product A mass,
Fermentation temperature is 30-37 DEG C, and the pH value for adjusting reaction with acid-base modifier is 5-8, and in 10-40%, rotating speed is for dissolved oxygen control
100-250r/min, ventilation should maintain 0.02-0.08Mpa in 500-2000L/min, fermentation tank pressure, and fermentation time is 8-
24h, obtains product B；
4) products C is prepared：The pH of adjustment product B is to 4.0～5.5, and temperature in fermentation tank is cooled to 0 DEG C～5 DEG C, protects
Deposit 2～5 days, then recover temperature to 20 DEG C～35 DEG C, obtain products C；
5) product D is prepared：Seed liquor Y is inoculated in products C, 10%-20% of the seed liquor Y consumption for products C quality,
Fermentation temperature is 30-34 DEG C, and the pH value for adjusting reaction with acid-base modifier is 5-8, and in 10-40%, rotating speed is for dissolved oxygen control
100-250r/min, ventilation should maintain 0.02-0.08Mpa in 500-2000L/min, fermentation tank pressure, and fermentation time is 8-
24h, obtains product D；
6) product E is prepared：The pH of adjustment product D is to 4.0～5.5, and temperature in fermentation tank is cooled to 0 DEG C～5 DEG C, protects
Deposit 2～7 days, then recover temperature to 25 DEG C～35 DEG C, obtain product E；
7) product F is prepared：Seed liquor Z is inoculated in product E, 10%-20% of the seed liquor Z consumption for product E quality,
Fermentation temperature is 30-34 DEG C, and the pH value for adjusting reaction with acid-base modifier is 5-8, and in 10-40%, rotating speed is for dissolved oxygen control
100-250r/min, ventilation should maintain 0.02-0.08Mpa in 500-2000L/min, fermentation tank pressure, and fermentation time is 8-
24h, obtains product F；
8) product G is prepared：The pH of adjustment product F is to 4.0～5.5, and temperature in fermentation tank is cooled to 0 DEG C～5 DEG C, protects
Deposit 2～7 days, then recover temperature to 25 DEG C～35 DEG C, obtain product G；G is dried to obtain Sargassum composite functional bacterial agent；
9) Sargassum granular potash fertilizer is prepared：The Sargassum composite functional bacterial agent of 5-10 parts is added in 100 parts of potash fertilizer raw material, and
Mix homogeneously, is lamellar through roll squeezer roll-forming；Pelletize is carried out using reaction type pelletize mode, is obtained final product into after drying of sieving
Product；
The potash fertilizer raw material is the one kind in potassium chloride, potassium sulfate, potassium nitrate, potassium dihydrogen phosphate and dipotassium hydrogen phosphate or appoints
Meaning ratio two or more.
Step 2) in bacillus cereuss activation with the culture medium prescription for spreading cultivation be：Bean cake powder 20g, yeast extract 6g, sea
Sodium alginate 1.2g, bovine bile 0.05g, potassium dihydrogen phosphate 0.04g, sodium chloride 0.2g, magnesium sulfate 0.2g, Mannitol 9.5g, water
1000mL, adjusts pH6.8-7.0, and the condition of spreading cultivation is：33 DEG C of culture 20h.
Step 2) in bacillus megaterium activation with the culture medium prescription for spreading cultivation be：Sweet potato powder 22g, sodium alginate
0.03g, Semen Maydis pulp 5g, peptone 11g, manganese dioxide 0.02g, potassium dihydrogen phosphate 0.02g, ferrous sulfate 0.2g, water 1000mL,
PH7.2-7.5 is adjusted, the condition of spreading cultivation is：31 DEG C of culture 20h.
Step 2) in bacillus amyloliquefaciens activation with the culture medium prescription for spreading cultivation be：Starch 20g, bean cake 40g, yeast
Cream 6g, potassium dihydrogen phosphate 2.0g, sodium acetate 5.0g, manganese sulfate 0.5g, magnesium sulfate 0.5g, Calcium Carbonate 0.3g, water 1000ml are adjusted
PH6.8-7.0, the condition of spreading cultivation is：35 DEG C of culture 24h.
Step 3) to step 8) in acid-base modifier be citric acid, oxalic acid, acetic acid, dipotassium hydrogen phosphate, sodium carbonate, acetic acid
One or more in sodium.
Step 1) in marine algae plant be Sargassum phyllocystum Tseng et Lu,Sargassum horneri (Turn.) C. Ag. (Fucus horneri (Turn.)C.Ag.,Spongocarpus horneri Kutz.), Thallus Laminariae (Thallus Eckloniae), Brown algae, sargassum thunbergii and Alga Sgrgassi Enervess in one or any
Ratio two or more.
The bacillus megaterium is bacillus megaterium YIC～BM1, and preserving number is CGMCC No.12156.
The present invention has following Advantageous Effects：
1. the strain that the present invention is adopted has high-salt tolerance, directly can compound with inorganic salt, overcome traditional microbial inoculum and exist
During hypersaline environment long-term storage the drawbacks of mortality, the shelf-life of fertilizer is substantially prolongs.And the environment of this high salt
Prevent other miscellaneous bacterias greatly reduce miscellaneous bacteria rate from surviving, it is ensured that the high-quality of fertilizer.
2. the present invention is fermented using composite bacteria, and all kinds of bacterial strains play synergism, and Sargassum active substance extraction ratio is high, has
Beneficial secondary metabolite abundant species；And cleverly apply staged fermentation, it is to avoid vying each other between microorganism species is short of money
Anti-, the effect of respective flora is performed to maximization, product fertilizer efficiency is better than single strain fermentation.
3. microbial bacterial agent is compounded with chemical fertilizer by the present invention using special reaction type granulating technique, whole process temperature
And environmental protection, it is to avoid high temperature and chemical reagent are ensure that to greatest extent to microorganism and the destruction of Sargassum active component
The survival rate of microorganism and Sargassum activity.
4. the product of the present invention plays synergism rich in multiple to beneficial microbe between strain, improves environment and adapts to
Ability, can in soil amount reproduction, effectively lift the content of microorganisms in soil, play buffering soil acidity or alkalinity, strengthen
Plant resistance to environment stress, the effect of improved soil environment.
5. the present invention introduces the functions such as Sargassum extract, beneficial complex micro organism fungicide on the basis of traditional potash fertilizer
Property composition, is the brand-new upgrading products of conventional fertilizers, and environmental-protecting performance and fertilizer efficiency are all increased dramatically.
Description of the drawings
Fig. 1 is the Gram’s staining of microexamination YIC～BM1 bacterial strains and assembles agglomerating phenomenon figure.
YIC～BM1 bacterial strain phylogenetic tree schematic diagrams of the Fig. 2 based on 16SrDNA sequence constructs.
Fig. 3 is the schematic diagram of impact of the different temperatures to YIC～BM1 strain enzyme-producings.
Fig. 4 is impact schematic diagrams of the different pH to YIC～BM1 strain enzyme-producings.
Specific embodiment
In conjunction with specific embodiments technical scheme is described in further detail, but not as to the present invention's
Limit.
Embodiment 1：
1) product A is prepared：By fresh Thallus Laminariae (Thallus Eckloniae) and Alga Sgrgassi Enervess, (mass ratio is 1:1) clean, remove impurity, after immersion 20h, mill
Slurry, proceeds in fermentation tank, 110 DEG C of sterilizing 30min, is cooled to 20 DEG C and obtains product A；
2) seed liquor is prepared：Bacillus cereuss are activated and is spread cultivation, prepare bacillus cereuss seed liquor X；Will be huge
Bacillus cereuss are activated and are spread cultivation, and prepare bacillus megaterium seed liquor Y；Bacillus amyloliquefaciens are activated and is spread cultivation, prepare solution
Bacillus amyloliquefacienses seed liquor Z；
Described bacillus cereuss activate：Bean cake powder 20g, yeast extract 6g, alginic acid
Sodium 1.2g, bovine bile 0.05g, potassium dihydrogen phosphate 0.04g, sodium chloride 0.2g, magnesium sulfate 0.2g, Mannitol 9.5g, water 1000mL,
PH6.8-7.0 is adjusted, the condition of spreading cultivation is：33 DEG C of culture 20h.
Described bacillus megaterium activates：Sweet potato powder 22g, sodium alginate 0.03g are beautiful
Rice ＆ peanut milk 5g, peptone 11g, manganese dioxide 0.02g, potassium dihydrogen phosphate 0.02g, ferrous sulfate 0.2g, water 1000mL, are adjusted
PH7.2-7.5, the condition of spreading cultivation is：31 DEG C of culture 20h.
Described bacillus amyloliquefaciens are activated：Starch 20g, bean cake 40g, yeast extract
6g, potassium dihydrogen phosphate 2.0g, sodium acetate 5.0g, manganese sulfate 0.5g, magnesium sulfate 0.5g, Calcium Carbonate 0.3g, water 1000ml are adjusted
PH6.8-7.0, the condition of spreading cultivation is：35 DEG C of culture 24h.
3) product B is prepared：Seed liquor X is inoculated in product A, seed liquor X consumption for product A mass 10%, fermentation
Temperature is 32 DEG C, and the pH value for adjusting reaction with acid-base modifier is 6.8, and dissolved oxygen is controlled 20%, and rotating speed is 200r/min, ventilation
Amount should maintain 0.04Mpa in 800L/min, fermentation tank pressure, and fermentation time is 22h, obtains product B；
4) products C is prepared：The pH of adjustment product B is to 4.5, and temperature in fermentation tank is cooled to 4 DEG C, preservation 3 days, then
Temperature is recovered to 20 DEG C, obtains products C；
5) product D is prepared：Seed liquor Y is inoculated in products C, seed liquor Y consumption for products C quality 12%, fermentation
Temperature is 34 DEG C, and the pH value for adjusting reaction with acid-base modifier is 7.0, and dissolved oxygen is controlled 30%, and rotating speed is 180r/min, ventilation
Amount should maintain 0.03Mpa in 750L/min, fermentation tank pressure, and fermentation time is 18h, obtains product D；
6) product E is prepared：The pH of adjustment product D is to 4.5, and temperature in fermentation tank is cooled to 3 DEG C, preservation 2 days, then
Temperature is recovered to 25 DEG C, obtains product E；
7) product F is prepared：Seed liquor Z is inoculated in product E, seed liquor Z consumption for product E quality 10%, fermentation
Temperature is 30 DEG C, and the pH value for adjusting reaction with acid-base modifier is 7.2, and dissolved oxygen is controlled 25%, and rotating speed is 200r/min, ventilation
Amount should maintain 0.03Mpa in 900L/min, fermentation tank pressure, and fermentation time is 18h, obtains product F；
8) product G is prepared：The pH of adjustment product F is to 5.0, and temperature in fermentation tank is cooled to 5 DEG C, preservation 2 days, then
Temperature is recovered to 35 DEG C, obtains product G；G is dried to obtain Sargassum composite functional bacterial agent.
9) Sargassum granular potash fertilizer is prepared：8 parts of Sargassum composite functional bacterial agent is added in 100 parts of potassium sulfate, and is mixed
Uniformly, through roll squeezer roll-forming be lamellar；Pelletize is carried out using reaction type pelletize mode, is got product after drying of sieving.
Embodiment 2：
1) product A is prepared：By clean for fresh Sargassum phyllocystum Tseng et Lu,Sargassum horneri (Turn.) C. Ag. (Fucus horneri (Turn.)C.Ag.,Spongocarpus horneri Kutz.), remove impurity, after immersion 24h, defibrination is proceeded in fermentation tank, and 120 DEG C go out
Bacterium 15min, is cooled to 20 DEG C and obtains product A；
2) seed liquor is prepared：Operational approach is same as Example 1.
3) product B is prepared：Seed liquor X is inoculated in product A, seed liquor X consumption for product A mass 12%, fermentation
Temperature is 32 DEG C, and the pH value for adjusting reaction with acid-base modifier is 6.6, and dissolved oxygen is controlled 25%, and rotating speed is 180r/min, ventilation
Amount should maintain 0.03Mpa in 850L/min, fermentation tank pressure, and fermentation time is 21h, obtains product B；
4) products C is prepared：The pH of adjustment product B is to 4.5, and temperature in fermentation tank is cooled to 4 DEG C, preservation 1 day, then
Temperature is recovered to 25 DEG C, obtains products C；
5) product D is prepared：Seed liquor Y is inoculated in products C, seed liquor Y consumption for products C quality 10%, fermentation
Temperature is 34 DEG C, and the pH value for adjusting reaction with acid-base modifier is 7.0, and dissolved oxygen is controlled 30%, and rotating speed is 200r/min, ventilation
Amount should maintain 0.04Mpa in 800L/min, fermentation tank pressure, and fermentation time is 20h, obtains product D；
6) product E is prepared：The pH of adjustment product D is to 5.0, and temperature in fermentation tank is cooled to 5 DEG C, preservation 3 days, then
Temperature is recovered to 25 DEG C, obtains product E；
7) product F is prepared：Seed liquor Z is inoculated in product E, seed liquor Z consumption for product E quality 15%, fermentation
Temperature is 30 DEG C, and the pH value for adjusting reaction with acid-base modifier is 6.8, and dissolved oxygen is controlled 30%, and rotating speed is 200r/min, ventilation
Amount should maintain 0.03Mpa in 750L/min, fermentation tank pressure, and fermentation time is 24h, obtains product F；
8) product G is prepared：The pH of adjustment product F is to 4.5, and temperature in fermentation tank is cooled to 5 DEG C, preservation 2 days, then
Temperature is recovered to 25 DEG C, obtains product G；G is dried to obtain Sargassum composite functional bacterial agent.
9) Sargassum granular potash fertilizer is prepared：To 100 parts of potassium chloride and potassium sulfate, (mass ratio is 1:1) add in mixture
8 parts of Sargassum composite functional bacterial agent, and mix homogeneously, are lamellar through roll squeezer roll-forming；Entered using reaction type pelletize mode
Row pelletize, gets product after drying of sieving.
Comparative example 1：
Ferment only with single culture.
1) product A is prepared：By clean for fresh sargassum thunbergii, remove impurity, after immersion 30h, defibrination is proceeded in fermentation tank, 120 DEG C
Sterilizing 20min, is cooled to 30 DEG C and obtains product A；
2) seed liquor is prepared：Bacillus cereuss are activated and is spread cultivation, prepare bacillus cereuss seed liquor X；
Described bacillus cereuss activate：Bean cake powder 20g, yeast extract 6g, alginic acid
Sodium 1.2g, bovine bile 0.05g, potassium dihydrogen phosphate 0.04g, sodium chloride 0.2g, magnesium sulfate 0.2g, Mannitol 9.5g, water 1000mL,
PH6.8-7.0 is adjusted, the condition of spreading cultivation is：33 DEG C of culture 20h.
3) product B is prepared：Seed liquor X is inoculated in product A, seed liquor X consumption for product A mass 10%, fermentation
Temperature is 32 DEG C, and the pH value for adjusting reaction with acid-base modifier is 6.8, and dissolved oxygen is controlled 20%, and rotating speed is 200r/min, ventilation
Amount should maintain 0.04Mpa in 800L/min, fermentation tank pressure, and fermentation time is 22h, obtains product B；
4) products C is prepared：The pH of adjustment product B is to 4.5, and temperature in fermentation tank is cooled to 4 DEG C, preservation 3 days, then
Temperature is recovered to 20 DEG C, obtains products C；C is dried to obtain Sargassum composite functional bacterial agent.
5) Sargassum granular potash fertilizer is prepared：8 parts of Sargassum composite functional bacterial agent is added in 100 parts of potassium sulfate, and is mixed
Uniformly, through roll squeezer roll-forming be lamellar；Pelletize is carried out using reaction type pelletize mode, is got product after drying of sieving.
Comparative example 2：
Sargassum is directly compounded with potash fertilizer without everfermentation
1) product A is prepared：By fresh Thallus Laminariae (Thallus Eckloniae) and Alga Sgrgassi Enervess, (mass ratio is 1:1) clean, remove impurity, after immersion 20h, mill
Slurry, proceeds in fermentation tank, 110 DEG C of extraction 30min, is cooled to 20 DEG C and obtains product A, A is dried and prepares Sargassum leaching powder B；
2) Sargassum granular potash fertilizer is prepared：To 100 parts of potassium chloride and potassium sulfate, (mass ratio is 1:1) add in mixture
8 parts of Sargassum leaching powder B, and mix homogeneously, are lamellar through roll squeezer roll-forming；Pelletize is carried out using reaction type pelletize mode,
Sieve and get product after drying.
Product index testing result is shown in Table 1.
Table 1：Product checking result
Wherein traditional potash fertilizer uses agriculture level potassium sulfate.
As a result show：It is different from traditional potash fertilizer, the not only effective viable bacteria containing high-load in product prepared by the present invention,
And abundant Sargassum extract, including alginic acid and brown alga oligose；The viable count and Sargassum extract of composite ratings fermentation
Content higher than single culture fermentation；The content of Sargassum active substance is greatly improved Sargassum afterwards by fermentation.
Embodiments of the invention and comparative example related fertilizer are imposed on corn planting plot, mu consumption is 50kg.Planting season
For half a year, conventional weeding, deinsectization, Rational Irrigation, guarantee adequate water, it is compared in N, P same level.With traditional potash fertilizer
As a control group, as a result as shown in table 2.
Table 2：Corn fertilizing contrast and experiment
Experiment shows, using the Semen Maydiss for implementing 1 sample, improves crop yield, compare traditional potash fertilizer and achieve 12%
Increase income, and reduce the pest and disease damage of crop.The content of Soil Microorganism is significantly improved, the pH of soil is effectively adjusted
Value, reduces the soil weight, effectively improves soil function.
Embodiment and the as shown by data of comparative example, the volume increase disease resisting effect after Sargassum is processed by fermentation are significantly lifted,
Beneficial to the maximization for realizing that Sargassum is worth；And composite ratings fermentation fertilizer efficiency is fermented better than single culture.
The preserving number of the bacillus megaterium YIC～BM1 is CGMCC No.12156；Depositary institution's title is：China
Microbiological Culture Collection administration committee common micro-organisms center；Depositary institution address is：BeiChen West Road, Chaoyang District, BeiJing City 1
Institute 3；Preservation date：On March 2nd, 2016；Latin Classification And Nomenclature is：Bacillus megaterium.
The biological nature of the bacillus megaterium YIC～BM1 is as follows：
Cellulose culture medium：Carboxymethyl cellulose sodium 15g, ammonium nitrate 1g, magnesium sulfate 1g, potassium dihydrogen phosphate 2g, agar
20g, distilled water 1000ml.
Liquid fermentation medium：Carboxymethyl cellulose sodium 10g, peptone 3g, yeast powder 0.5g, ammonium nitrate 2g, magnesium sulfate
0.3g, potassium dihydrogen phosphate 2g, calcium chloride 0.3g, distilled water 1000ml, pH7.0-7.4.
After YIC～BM1 bacterial strains grow 48h on cellulose culture medium flat plate, bacterium colony is circular, white, surfacing.Micro-
Sem observation bacterial strain is Gram-positive, shaft-like, can move, and produces spore.20～40 DEG C of growth temperature range, PH scopes 6-10,
NaCl concentration scope 0～20%, 37 DEG C of bacterial strain optimum growth temperature, the most suitable growth pH 7.5.Catalase, oxidase, galactoside
Enzyme positive, indole, VP experiments are negative, do not go back orthonitric acid；Can utilize glucose, mannose, Lactose, Raffinose, sucrose, I
Uncle's sugar, xylose, Mannitol, Sorbitol.According to its physiological and biochemical property, it is bacillus megaterium Bacillus by the identification of strains
megaterium.
Fig. 1 finds out that YIC～BM1 bacterial strains are gram positive bacteria；
Fig. 2 finds out that the sibship of YIC～BM1 bacterial strains is nearest with bacillus megaterium；
Fig. 3 finds out that the most suitable producing enzyme temperature of YIC～BM1 bacterial strains is 35 DEG C；
Fig. 4 finds out that optimal producing enzyme pH of YIC～BM1 bacterial strains is 6.0.
Reaction type pelletize mode of the present invention refers to " multiple using the Chinese patent of Publication No. " CN 102775255 "
The Granulation Equipments announced by complex fertilizer counterattack comminutor and the complex fertilizer producing line using the counterattack comminutor " carry out pelletize.
Claims (8)
1. a kind of method that compound microorganism ferments prepare Sargassum granular potash fertilizer, it is characterised in that comprise the following steps：
1）Prepare product A：By clean for fresh marine algae plant, remove impurity, after immersion 12-48h, defibrination is proceeded in fermentation tank,
110-150 DEG C of sterilizing 15-30min, is cooled to 20-35 DEG C and obtains product A；
2）Prepare seed liquor：Bacillus cereuss are activated and is spread cultivation, prepare bacillus cereuss seed liquor X；By huge spore
Bacillus activates and spreads cultivation, and prepares bacillus megaterium seed liquor Y；Bacillus amyloliquefaciens are activated and is spread cultivation, prepare solution starch
Bacillus cereuss seed liquor Z；
3）Prepare product B：Seed liquor X is inoculated in product A, 10%-20% of the seed liquor X consumption for product A mass, fermentation temperature
Spend for 30-37 DEG C, the pH value for adjusting reaction with acid-base modifier is 5-8, and in 10-40%, rotating speed is 100-250r/ for dissolved oxygen control
Min, ventilation should maintain 0.02-0.08Mpa in 500-2000L/min, fermentation tank pressure, and fermentation time is 8-24h, is produced
Product B；
4）Prepare products C：The pH of adjustment product B is to 4.0～5.5, and temperature in fermentation tank is cooled to 0 DEG C～5 DEG C, preserves 2
～5 days, then temperature is recovered to 20 DEG C～35 DEG C, obtains products C；
5）Prepare product D：Seed liquor Y is inoculated in products C, 10%-20% of the seed liquor Y consumption for products C quality, fermentation temperature
Spend for 30-34 DEG C, the pH value for adjusting reaction with acid-base modifier is 5-8, and in 10-40%, rotating speed is 100-250r/ for dissolved oxygen control
Min, ventilation should maintain 0.02-0.08Mpa in 500-2000L/min, fermentation tank pressure, and fermentation time is 8-24h, is produced
Product D；
6）Prepare product E：The pH of adjustment product D is to 4.0～5.5, and temperature in fermentation tank is cooled to 0 DEG C～5 DEG C, preserves 2
～7 days, then temperature is recovered to 25 DEG C～35 DEG C, obtains product E；
7）Prepare product F：Seed liquor Z is inoculated in product E, 10%-20% of the seed liquor Z consumption for product E quality, fermentation temperature
Spend for 30-34 DEG C, the pH value for adjusting reaction with acid-base modifier is 5-8, and in 10-40%, rotating speed is 100-250r/ for dissolved oxygen control
Min, ventilation should maintain 0.02-0.08Mpa in 500-2000L/min, fermentation tank pressure, and fermentation time is 8-24h, is produced
Product F；
8）Prepare product G：The pH of adjustment product F is to 4.0～5.5, and temperature in fermentation tank is cooled to 0 DEG C～5 DEG C, preserves 2
～7 days, then temperature is recovered to 25 DEG C～35 DEG C, obtains product G；G is dried to obtain Sargassum composite functional bacterial agent；
9）Prepare Sargassum granular potash fertilizer：The Sargassum composite functional bacterial agent of 5-10 parts is added in 100 parts of potash fertilizer raw material, and is mixed
Uniformly, through roll squeezer roll-forming be lamellar；Pelletize is carried out using reaction type pelletize mode, is got product after drying of sieving；
The potash fertilizer raw material is the one kind in potassium chloride, potassium sulfate, potassium nitrate, potassium dihydrogen phosphate and dipotassium hydrogen phosphate or arbitrarily compares
Example two or more.
2. the method that compound microorganism ferments as claimed in claim 1 prepare Sargassum granular potash fertilizer, it is characterised in that：Step 2）
In bacillus cereuss activation with the culture medium prescription for spreading cultivation be：Bean cake powder 20g, yeast extract 6g, sodium alginate 1.2g, Fel Bovis seu Bubali
Salt 0.05g, potassium dihydrogen phosphate 0.04g, sodium chloride 0.2g, magnesium sulfate 0.2g, Mannitol 9.5g, water 1000mL adjust pH6.8-
7.0, the condition of spreading cultivation is：33 DEG C of culture 20h.
3. the method that compound microorganism ferments as claimed in claim 1 prepare Sargassum granular potash fertilizer, it is characterised in that：Step 2）
In bacillus megaterium activation with the culture medium prescription for spreading cultivation be：Sweet potato powder 22g, sodium alginate 0.03g, Semen Maydis pulp 5g, egg
White peptone 11g, manganese dioxide 0.02g, potassium dihydrogen phosphate 0.02g, ferrous sulfate 0.2g, water 1000mL, adjust pH7.2-7.5, expand
Training condition is：31 DEG C of culture 20h.
4. the method that compound microorganism ferments as claimed in claim 1 prepare Sargassum granular potash fertilizer, it is characterised in that：Step 2）
In bacillus amyloliquefaciens activation with the culture medium prescription for spreading cultivation be：Starch 20g, bean cake 40g, yeast extract 6g, biphosphate
Potassium 2.0g, sodium acetate 5.0g, manganese sulfate 0.5g, magnesium sulfate 0.5g, Calcium Carbonate 0.3g, water 1000ml adjust pH6.8-7.0, expand
Training condition is：35 DEG C of culture 24h.
5. the method that compound microorganism ferments as claimed in claim 1 prepare Sargassum granular potash fertilizer, it is characterised in that：Step 3）
To step 8）In acid-base modifier be citric acid, oxalic acid, acetic acid, dipotassium hydrogen phosphate, sodium carbonate, the one kind or several in sodium acetate
Kind.
6. the method that the compound microorganism ferments as described in claim 1 to 5 any one prepare Sargassum granular potash fertilizer, its feature
It is：Step 1）In marine algae plant be Sargassum phyllocystum Tseng et Lu,Sargassum horneri (Turn.) C. Ag. (Fucus horneri (Turn.)C.Ag.,Spongocarpus horneri Kutz.), Thallus Laminariae (Thallus Eckloniae), Brown algae, sargassum thunbergii and Alga Sgrgassi Enervess in one or any ratio
Example two or more.
7. the method that the compound microorganism ferments as described in claim 1 to 5 any one prepare Sargassum granular potash fertilizer, its feature
It is：The bacillus megaterium is bacillus megaterium YIC～BM1, and preserving number is CGMCC No.12156.
8. the method that compound microorganism ferments as claimed in claim 6 prepare Sargassum granular potash fertilizer, it is characterised in that：Described huge
Bacterium anthracoides are bacillus megaterium YIC～BM1, and preserving number is CGMCC No.12156.
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| CN102775255A (en) * | 2012-08-07 | 2012-11-14 | 五洲丰农业科技有限公司 | Compound fertilizer impact granulator and compound fertilizer production line using same |
| CN104262008A (en) * | 2014-09-30 | 2015-01-07 | 青岛嘉瑞生物技术有限公司 | Process for preparing liquid seaweed fertilizer through enzymolysis and fermentation |
| CN104447008A (en) * | 2014-12-05 | 2015-03-25 | 中国科学院烟台海岸带研究所 | Method for preparing liquid seaweed fertilizer |
| CN104774094A (en) * | 2015-04-01 | 2015-07-15 | 大连理工大学 | Preparation method of compound microbial seaweed fertilizer |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102775255A (en) * | 2012-08-07 | 2012-11-14 | 五洲丰农业科技有限公司 | Compound fertilizer impact granulator and compound fertilizer production line using same |
| CN104262008A (en) * | 2014-09-30 | 2015-01-07 | 青岛嘉瑞生物技术有限公司 | Process for preparing liquid seaweed fertilizer through enzymolysis and fermentation |
| CN104447008A (en) * | 2014-12-05 | 2015-03-25 | 中国科学院烟台海岸带研究所 | Method for preparing liquid seaweed fertilizer |
| CN104774094A (en) * | 2015-04-01 | 2015-07-15 | 大连理工大学 | Preparation method of compound microbial seaweed fertilizer |
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