CN104262008A - Process for preparing liquid seaweed fertilizer through enzymolysis and fermentation - Google Patents
Process for preparing liquid seaweed fertilizer through enzymolysis and fermentation Download PDFInfo
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- CN104262008A CN104262008A CN201410514569.XA CN201410514569A CN104262008A CN 104262008 A CN104262008 A CN 104262008A CN 201410514569 A CN201410514569 A CN 201410514569A CN 104262008 A CN104262008 A CN 104262008A
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- marine alga
- enzymolysis
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- 239000003337 fertilizer Substances 0.000 title claims abstract description 49
- 238000000855 fermentation Methods 0.000 title claims abstract description 48
- 230000004151 fermentation Effects 0.000 title claims abstract description 46
- 239000007788 liquid Substances 0.000 title claims abstract description 36
- 241001474374 Blennius Species 0.000 title claims abstract description 23
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 19
- 239000000126 substance Substances 0.000 claims abstract description 12
- 239000011573 trace mineral Substances 0.000 claims abstract description 10
- 235000013619 trace mineral Nutrition 0.000 claims abstract description 10
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 8
- 239000005017 polysaccharide Substances 0.000 claims abstract description 8
- 241000894006 Bacteria Species 0.000 claims abstract description 7
- 150000001875 compounds Chemical class 0.000 claims abstract description 6
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 22
- 102000004190 Enzymes Human genes 0.000 claims description 15
- 108090000790 Enzymes Proteins 0.000 claims description 15
- 241000196252 Ulva Species 0.000 claims description 13
- 230000007071 enzymatic hydrolysis Effects 0.000 claims description 13
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 11
- 229910052802 copper Inorganic materials 0.000 claims description 11
- 239000010949 copper Substances 0.000 claims description 11
- 229910052742 iron Inorganic materials 0.000 claims description 11
- 229910052700 potassium Inorganic materials 0.000 claims description 10
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 7
- 239000011701 zinc Substances 0.000 claims description 7
- 229910052725 zinc Inorganic materials 0.000 claims description 7
- 241000228245 Aspergillus niger Species 0.000 claims description 6
- 241000194107 Bacillus megaterium Species 0.000 claims description 6
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 claims description 6
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims description 6
- 239000004327 boric acid Substances 0.000 claims description 6
- 239000002131 composite material Substances 0.000 claims description 6
- 229910052748 manganese Inorganic materials 0.000 claims description 6
- 239000011572 manganese Substances 0.000 claims description 6
- 239000007787 solid Substances 0.000 claims description 6
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 claims description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 5
- 229910019142 PO4 Inorganic materials 0.000 claims description 5
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 5
- 229920002472 Starch Polymers 0.000 claims description 5
- 238000010564 aerobic fermentation Methods 0.000 claims description 5
- 229910052796 boron Inorganic materials 0.000 claims description 5
- 229940041514 candida albicans extract Drugs 0.000 claims description 5
- 229940038879 chelated zinc Drugs 0.000 claims description 5
- 238000010612 desalination reaction Methods 0.000 claims description 5
- 239000000428 dust Substances 0.000 claims description 5
- 239000008103 glucose Substances 0.000 claims description 5
- 239000012535 impurity Substances 0.000 claims description 5
- 238000011081 inoculation Methods 0.000 claims description 5
- 239000002054 inoculum Substances 0.000 claims description 5
- 230000014759 maintenance of location Effects 0.000 claims description 5
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 claims description 5
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 5
- 239000010452 phosphate Substances 0.000 claims description 5
- 239000011591 potassium Substances 0.000 claims description 5
- 238000002203 pretreatment Methods 0.000 claims description 5
- 239000008107 starch Substances 0.000 claims description 5
- 235000019698 starch Nutrition 0.000 claims description 5
- 239000006228 supernatant Substances 0.000 claims description 5
- 238000002604 ultrasonography Methods 0.000 claims description 5
- 239000012138 yeast extract Substances 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 3
- 238000005516 engineering process Methods 0.000 claims description 2
- 150000004676 glycans Chemical class 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 25
- 239000002689 soil Substances 0.000 abstract description 11
- 235000015097 nutrients Nutrition 0.000 abstract description 9
- 150000004804 polysaccharides Chemical class 0.000 abstract description 7
- 229920002521 macromolecule Polymers 0.000 abstract description 6
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 239000003895 organic fertilizer Substances 0.000 abstract description 3
- 235000010443 alginic acid Nutrition 0.000 abstract description 2
- 239000000783 alginic acid Substances 0.000 abstract description 2
- 229920000615 alginic acid Polymers 0.000 abstract description 2
- 229960001126 alginic acid Drugs 0.000 abstract description 2
- 150000004781 alginic acids Chemical class 0.000 abstract description 2
- 238000005904 alkaline hydrolysis reaction Methods 0.000 abstract description 2
- 230000004071 biological effect Effects 0.000 abstract description 2
- 230000006378 damage Effects 0.000 abstract description 2
- 230000000593 degrading effect Effects 0.000 abstract description 2
- 230000002708 enhancing effect Effects 0.000 abstract description 2
- 239000002207 metabolite Substances 0.000 abstract description 2
- -1 nitrogen-containing compound Chemical class 0.000 abstract description 2
- 239000003375 plant hormone Substances 0.000 abstract description 2
- 238000005903 acid hydrolysis reaction Methods 0.000 abstract 1
- 230000003115 biocidal effect Effects 0.000 abstract 1
- 150000002772 monosaccharides Chemical class 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 5
- 238000006460 hydrolysis reaction Methods 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- 239000002253 acid Substances 0.000 description 3
- 229940088623 biologically active substance Drugs 0.000 description 3
- 230000007073 chemical hydrolysis Effects 0.000 description 3
- 230000000050 nutritive effect Effects 0.000 description 3
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 239000000306 component Substances 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 description 1
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 1
- WKBOTKDWSSQWDR-UHFFFAOYSA-N Bromine atom Chemical compound [Br] WKBOTKDWSSQWDR-UHFFFAOYSA-N 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000195474 Sargassum Species 0.000 description 1
- LPQOADBMXVRBNX-UHFFFAOYSA-N ac1ldcw0 Chemical compound Cl.C1CN(C)CCN1C1=C(F)C=C2C(=O)C(C(O)=O)=CN3CCSC1=C32 LPQOADBMXVRBNX-UHFFFAOYSA-N 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- GDTBXPJZTBHREO-UHFFFAOYSA-N bromine Substances BrBr GDTBXPJZTBHREO-UHFFFAOYSA-N 0.000 description 1
- 229910052794 bromium Inorganic materials 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- 239000011630 iodine Substances 0.000 description 1
- 229910052740 iodine Inorganic materials 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000011785 micronutrient Substances 0.000 description 1
- 235000013369 micronutrients Nutrition 0.000 description 1
- 239000002366 mineral element Substances 0.000 description 1
- 231100000957 no side effect Toxicity 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000000661 sodium alginate Substances 0.000 description 1
- 235000010413 sodium alginate Nutrition 0.000 description 1
- 229940005550 sodium alginate Drugs 0.000 description 1
- 239000002681 soil colloid Substances 0.000 description 1
- 239000003516 soil conditioner Substances 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
- C05D9/02—Other inorganic fertilisers containing trace elements
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Soil Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a process for preparing a liquid seaweed fertilizer through enzymolysis and fermentation. The process disclosed by the invention can be used for generating a large quantity of soil beneficial bacteria and multiple special-effect metabolites by degrading macromolecular substances which are contained in seaweeds and difficult to utilize into micromolecular polysaccharide, monosaccharide and a nitrogen-containing compound in compound enzymolysis and multi-strain co-fermentation modes, thereby enhancing the biological activity of a seaweed fertilizer, preventing the destruction of strong base, high temperature and high pressure in the production process of the seaweed fertilizer and completely retaining abundant nutrient substances contained in the seaweeds. According to the process, the content of a product, namely alginic acid, is 40-60 g/L and is increased by more than 30% compared with that prepared from an enzymolysis method, an alkaline hydrolysis method, an acid hydrolysis method and a simple microbe fermentation method, and the effective viable count is more than or equal to 2 billion/mL; the product integrates natural nutrient components, antibiotic substances and plant hormones, combines the efficacy of three fertilizers, namely an organic fertilizer, a microbe fertilizer and a trace element fertilizer and has a wide application prospect.
Description
Technical field
The present invention relates to the technique that a kind of enzymatic hydrolysis and fermentation prepares seaweed liquid fertilizer, belong to field of fertilizer manufacturing.
Background technology
Marine alga is a kind of marine organisms of abundance, is widely used in food, industrial raw material, botanical.Marine alga is applied to agricultural and Animal husbandry production have centuries history.The new-type fertilizer that seaweed fertilizer is a class pure natural, have no side effect, it contains outside the abundant non-amino acid containing N organism and some amount, protein and micronutrient element, also containing mineral element and abundant vitamins C, the B such as zinc, bromine, iodine that the distinctive Sargassum polysaccharides of marine organisms, alginic acid, highly unsaturated fatty acids and terrestrial plant are rare
1, B
2, B
12, the multiple nutrients material such as D, E, K, there is effects such as improving crop yield, the drought resisting of oil recovery enhancement and enhancing crop, cold-resistant, disease resistance.Therefore, utilize marine alga to produce organic fertilizer and become one of important focus of current new-type fertilizer research.
The impact of production method on product biologically active substance and nutrient composition content of marine alga fertilizer is very big, even if the difference of the identical activity of content is also very large.The production method of seaweed fertilizer generally can be divided into chemical hydrolysis, physics extraction method, enzymolysis process, biological fermentation process etc., extracts biologically active substance in addition again through fertilizer that science is formulated from the waste of seaweed industry.Current most domestic producer mainly takes chemical hydrolysis production seaweed fertilizer, adopts the maximum inferior position of chemical method to be that highly basic high temperature can destroy the activity of marine alga endogenous substance; Physics extraction method is the object adopting the technique of high-temperature low-pressure cooling to reach the fragmentation of alginic cell wall, and be better than chemical hydrolysis, but working condition is harsh, production cost is higher, and industrialization transforms and there is larger difficulty.Biological fermentation ratio juris utilizes microorganism with marine alga etc. for the macromolecular substance being formed marine alga is degraded into small molecules, water miscible material by the multiple enzyme produced in the metabolic process of nutrient, because there is no highly basic and the high temperature of chemical method in fermenting process, high pressure and the low temperature of Physical is not had yet, therefore the biologically active substance in marine alga and nutritive substance is remained to greatest extent, and can be absorbed and used by plants, therefore biological fermentation process becomes the approach that marine alga fertilizer is produced gradually.But preparing marine alga fertilizer with biological fermentation process also exists a lot of defects at present, one is that organism of fermentation bacterial classification is single, the enzyme system produced is single, be difficult to really to degrade the macromolecular substance of marine alga, affect the action effect of marine alga fertilizer, two is that biological fermentation process is prepared marine alga fertilizer and also existed and adopt fermentable bacterial classification to soil without any beneficial effect, and simple adds production cost.Therefore adopt biological fermentation process to produce the enterprise of marine alga fertilizer at present and mostly adopt the complex method simultaneously adding soda acid, different exist destroy, the drawback of the nutritive element such as active factor in loss marine alga.
Summary of the invention
Prepare marine alga fertilizer existence for solution biological fermentation process and be difficult to marine alga macromolecular substance of thoroughly degrading, marine alga fertilizer effect is poor, the more high drawback of production cost, a kind of enzymatic hydrolysis and fermentation is the object of the present invention is to provide to prepare the technique of seaweed liquid fertilizer, adopt the mode of complex enzyme hydrolysis and multi-cultur es co-fermentation that the macromolecular substance being difficult in marine alga utilize is degraded to micromolecular polysaccharide, monose, nitrogenous compound, and produce a large amount of soil probioticss and multiple special efficacy meta-bolites, improve the biological activity of marine alga fertilizer, reduce the production cost that biological fermentation process produces marine alga fertilizer.
Above-mentioned purpose of the present invention is achieved by the following technical programs:
The invention provides the technique that a kind of enzymatic hydrolysis and fermentation prepares seaweed liquid fertilizer, it is characterized in that specifically comprising the following steps:
(1) marine alga pre-treatment: rinse marine alga with clear water, removes impurity, drains; With cutter, marine alga is cut into 3 ~ 5cm length; 0.1%Ca(OH with pH6.5 ~ 7.0)
2solution carries out 3 times to Enteromorpha and soaks, and each 15min, carries out desalination color retention; Dry under 85 DEG C of conditions, last abrasive dust, cross 40 mesh sieves;
(2) ultrasonic wave enzymolysis: add marine alga quality 8 ~ 10 times of water, adjustment pH6.8 ~ 7.2; 2 ~ 3% saccharifying enzyme, 1 ~ 2% non-starch polysaccharide enzyme is added, ultrasound-assisted enzymolysis 2 ~ 4h at 40 ~ 60 DEG C, ultrasonic power 400w, frequency 25kHz by marine alga mass ratio;
(3) fermented liquid allotment: add 3.0 ~ 5.0% glucose of marine alga mass ratio, 2.0 ~ 3.0% yeast extract pastes, 0.3 ~ 0.5% potassium primary phosphate, 0.02%MgSO in enzymolysis solution
4.7H
2o, 0.01%CaCl
2, pH is adjusted to 7.0 ~ 7.2;
(4) aerobic fermentation: select liquid fermentation tank, liquid amount is 60 ~ 70%(volume ratio), inoculation subtilis, bacillus megaterium and aspergillus niger, inoculum size is respectively 2 ~ 3%, 2 ~ 3%, 1 ~ 2%(volume ratio), control leavening temperature 28 ~ 37 DEG C, rotating speed 150 ~ 300rpm, air flow 10 ~ 20L/min; At this fermentation condition bottom fermentation 8 ~ 12h;
(5) filter: filter to obtain supernatant liquor after fermentation ends, solid substance enters production of compound fertilizer;
(6) composite: to add P and K decomposing bacterium and vinelandii respectively by fermented liquid mass ratio 3 ~ 5%; Add the trace elements such as copper, iron, zinc, manganese, boron, total amount is 30 ~ 40g/L.
Above-mentioned marine alga can be one or both the mixture in sea-tangle, Enteromorpha, sargassun.
Above-mentioned trace element be Chelating state, the mass ratio of chelated copper, chelated iron, chelated zinc, Chelated Manganese and boric acid is: 2 ~ 3:4 ~ 6:1 ~ 2:2 ~ 3:3 ~ 4.
The invention provides the technique that a kind of enzymatic hydrolysis and fermentation prepares seaweed liquid fertilizer, avoid the destruction of highly basic, high temperature, high pressure in marine alga fertilizer production process, by the various enzyme systems degraded marine alga macromolecular substance produced in complex enzyme hydrolysis fermentable metabolic process, remain nutritive substance abundant in marine alga completely.Product Lalgine content of the present invention 40 ~ 60g/L, improves more than 30%, living bacteria count >=2,000,000,000/mL than the enzymolysis process reported at present, alkaline hydrolysis, acid hydrolyzation and simple microbe fermentation method.
The invention provides the technique that a kind of enzymatic hydrolysis and fermentation prepares seaweed liquid fertilizer, this invention product integrates natural nutrient component, antibiotics, plant hormone, there is organic fertilizer, microbial fertilizer, trace element fertilizer " three is fertile " composite efficacy, one is be rich in subtilis, bacillus megaterium, aspergillus niger, and the exogenous soil beneficial microorganism added can promote to strengthen its absorbing soil nutrient, moisture and gas by the root system that plant management one stalwartness is grown; The vascular bundle cell of plant haulm can be increased, accelerate the transport of water, nutrient and photosynthate; Plant cell division can be promoted, effective Promoting plant growth; Two is that functional marine alga fertilizer provided by the invention is rich in sodium alginate, it is natural soil conditioner, be conducive to forming the indispensable bright and clear material-soil colloid of water-stable granular structure, promotion, improved soil nodule structure, improve soil internal voids, coordinate the ratio of solid, liquid, gas three in soil, strengthen soil organisms vigor, promote the release of available nutrient, improve soil to the resiliency of soda acid and fertilizer conservation, water-holding capacity; Three is the copper, iron, zinc, the trace element such as manganese and boric acid that with the addition of Chelating state in fertilizer product; Four be production reaction temperature and, functional active components loss little.
The invention provides the technique that a kind of enzymatic hydrolysis and fermentation prepares seaweed liquid fertilizer, functional marine alga fertilizer product can directly act on plant, also can be used for soil base manure.
Embodiment
embodiment 1:
Enzymatic hydrolysis and fermentation prepares a technique for seaweed liquid fertilizer, and concrete preparation process is as follows:
(1) sea-tangle pre-treatment: rinse sea-tangle with clear water, removes impurity, drains; With cutter, sea-tangle is cut into 3 ~ 5cm length; 0.1%Ca(OH with pH6.5 ~ 7.0)
2solution carries out 3 times to Enteromorpha and soaks, and each 15min, carries out desalination color retention; Dry under 85 DEG C of conditions, last abrasive dust, cross 40 mesh sieves;
(2) ultrasonic wave enzymolysis: add sea-tangle quality 8 times of water, adjustment pH6.8 ~ 7.2; 3% saccharifying enzyme, 2% non-starch polysaccharide enzyme is added, ultrasound-assisted enzymolysis 2h at 40 ~ 60 DEG C, ultrasonic power 400w, frequency 25kHz by sea-tangle mass ratio;
(3) fermented liquid allotment: add 3.0% glucose of sea-tangle mass ratio, 2.0% yeast extract paste, 0.3% potassium primary phosphate, 0.02%MgSO in enzymolysis solution
4.7H
2o, 0.01%CaCl
2, pH is adjusted to 7.0 ~ 7.2;
(4) aerobic fermentation: select liquid fermentation tank, liquid amount is 60 ~ 70%(volume ratio), inoculation subtilis, bacillus megaterium and aspergillus niger, inoculum size is respectively 2%, 3%, 2%(volume ratio), control leavening temperature 28 ~ 37 DEG C, rotating speed 150 ~ 300rpm, air flow 10 ~ 20L/min; At this fermentation condition bottom fermentation 12h;
(5) filter: filter to obtain supernatant liquor after fermentation ends, solid substance enters production of compound fertilizer;
(6) composite: to add P and K decomposing bacterium and vinelandii respectively by fermented liquid mass ratio 3%, 5%; Add the trace elements such as copper, iron, zinc, manganese, boron, total amount is 30g/L, and the mass ratio of chelated copper, chelated iron, chelated zinc, Chelated Manganese and boric acid is: 2:6:1:3:4.
embodiment 2:
Enzymatic hydrolysis and fermentation prepares a technique for seaweed liquid fertilizer, and concrete preparation process is as follows:
(1) Enteromorpha pre-treatment: rinse Enteromorpha with clear water, removes impurity, drains; With cutter, Enteromorpha is cut into 3 ~ 5cm length; 0.1%Ca(OH with pH6.5 ~ 7.0)
2solution carries out 3 times to Enteromorpha and soaks, and each 15min, carries out desalination color retention; Dry under 85 DEG C of conditions, last abrasive dust, cross 40 mesh sieves;
(2) ultrasonic wave enzymolysis: add Enteromorpha quality 9 times of water, adjustment pH6.8 ~ 7.2; 2% saccharifying enzyme, 1% non-starch polysaccharide enzyme is added, ultrasound-assisted enzymolysis 4h at 40 ~ 60 DEG C, ultrasonic power 400w, frequency 25kHz by Enteromorpha mass ratio;
(3) fermented liquid allotment: add 5.0% glucose of Enteromorpha mass ratio, 3.0% yeast extract paste, 0.5% potassium primary phosphate, 0.02%MgSO in enzymolysis solution
4.7H
2o, 0.01%CaCl
2, pH is adjusted to 7.0 ~ 7.2;
(4) aerobic fermentation: select liquid fermentation tank, liquid amount is 60 ~ 70%(volume ratio), inoculation subtilis, bacillus megaterium and aspergillus niger, inoculum size is respectively 3%, 2%, 2%(volume ratio), control leavening temperature 28 ~ 37 DEG C, rotating speed 150 ~ 300rpm, air flow 10 ~ 20L/min; At this fermentation condition bottom fermentation 8h;
(5) filter: filter to obtain supernatant liquor after fermentation ends, solid substance enters production of compound fertilizer;
(6) composite: to add P and K decomposing bacterium and vinelandii respectively by fermented liquid mass ratio 5%, 3%; Add the trace elements such as copper, iron, zinc, manganese, boron, total amount is 35g/L, and the mass ratio of chelated copper, chelated iron, chelated zinc, Chelated Manganese and boric acid is: 3:4:2:2:3.
embodiment 3:
Enzymatic hydrolysis and fermentation prepares a technique for seaweed liquid fertilizer, and concrete preparation process is as follows:
(1) sargassun pre-treatment: rinse sargassun with clear water, removes impurity, drains; With cutter, sargassun is cut into 3 ~ 5cm length; 0.1%Ca(OH with pH6.5 ~ 7.0)
2solution carries out 3 times to Enteromorpha and soaks, and each 15min, carries out desalination color retention; Dry under 85 DEG C of conditions, last abrasive dust, cross 40 mesh sieves;
(2) ultrasonic wave enzymolysis: add sargassun quality 10 times of water, adjustment pH6.8 ~ 7.2; 2.5% saccharifying enzyme, 1.5% non-starch polysaccharide enzyme is added, ultrasound-assisted enzymolysis 3h at 40 ~ 60 DEG C, ultrasonic power 400w, frequency 25kHz by sargassun mass ratio;
(3) fermented liquid allotment: add 4.0% glucose of sargassun mass ratio, 2.5% yeast extract paste, 0.4% potassium primary phosphate, 0.02%MgSO in enzymolysis solution
4.7H
2o, 0.01%CaCl
2, pH is adjusted to 7.0 ~ 7.2;
(4) aerobic fermentation: select liquid fermentation tank, liquid amount is 60 ~ 70%(volume ratio), inoculation subtilis, bacillus megaterium and aspergillus niger, inoculum size is respectively 2.5%, 2.5%, 1.5%(volume ratio), control leavening temperature 28 ~ 37 DEG C, rotating speed 150 ~ 300rpm, air flow 10 ~ 20L/min; At this fermentation condition bottom fermentation 10h;
(5) filter: filter to obtain supernatant liquor after fermentation ends, solid substance enters production of compound fertilizer;
(6) composite: to add P and K decomposing bacterium and vinelandii respectively by fermented liquid mass ratio 4%, 4%; Add the trace elements such as copper, iron, zinc, manganese, boron, total amount is 40g/L, and the mass ratio of chelated copper, chelated iron, chelated zinc, Chelated Manganese and boric acid is: 2:4:1:3:4.
Above embodiment only for illustration of technical scheme of the present invention, but not is limited; Although be described in detail by invention with reference to previous embodiment, for the person of ordinary skill of the art, still the technical scheme described in previous embodiment can be modified, or equivalent replacement has been carried out to wherein portion of techniques feature; And to these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of the present invention's technical scheme required for protection.
Claims (3)
1. enzymatic hydrolysis and fermentation prepares a seaweed liquid fertilizer, it is characterized in that preparation technology specifically comprises the following steps:
(1) marine alga pre-treatment: rinse marine alga with clear water, removes impurity, drains; With cutter, marine alga is cut into 3 ~ 5cm length; 0.1%Ca(OH with pH6.5 ~ 7.0)
2solution carries out 3 times to Enteromorpha and soaks, and each 15min, carries out desalination color retention; Dry under 85 DEG C of conditions, last abrasive dust, cross 40 mesh sieves;
(2) ultrasonic wave enzymolysis: add marine alga quality 8 ~ 10 times of water, adjustment pH6.8 ~ 7.2; 2 ~ 3% saccharifying enzyme, 1 ~ 2% non-starch polysaccharide enzyme is added, ultrasound-assisted enzymolysis 2 ~ 4h at 40 ~ 60 DEG C, ultrasonic power 400w, frequency 25kHz by marine alga mass ratio;
(3) fermented liquid allotment: add 3.0 ~ 5.0% glucose of marine alga mass ratio, 2.0 ~ 3.0% yeast extract pastes, 0.3 ~ 0.5% potassium primary phosphate, 0.02%MgSO in enzymolysis solution
4.7H
2o, 0.01%CaCl
2, pH is adjusted to 7.0 ~ 7.2;
(4) aerobic fermentation: select liquid fermentation tank, liquid amount is 60 ~ 70%(volume ratio), inoculation subtilis, bacillus megaterium and aspergillus niger, inoculum size is respectively 2 ~ 3%, 2 ~ 3%, 1 ~ 2%(volume ratio), control leavening temperature 28 ~ 37 DEG C, rotating speed 150 ~ 300rpm, air flow 10 ~ 20L/min; At this fermentation condition bottom fermentation 8 ~ 12h;
(5) filter: filter to obtain supernatant liquor after fermentation ends, solid substance enters production of compound fertilizer;
(6) composite: to add P and K decomposing bacterium and vinelandii respectively by fermented liquid mass ratio 3 ~ 5%; Add the trace elements such as copper, iron, zinc, manganese, boron, total amount is 30 ~ 40g/L.
2. a kind of enzymatic hydrolysis and fermentation according to claim 1 prepares seaweed liquid fertilizer, it is characterized in that described marine alga is one or both the mixture in sea-tangle, Enteromorpha, sargassun.
3. a kind of enzymatic hydrolysis and fermentation according to claim 1 prepares seaweed liquid fertilizer, and it is characterized in that described trace element is Chelating state, the mass ratio of chelated copper, chelated iron, chelated zinc, Chelated Manganese and boric acid is: 2 ~ 3:4 ~ 6:1 ~ 2:2 ~ 3:3 ~ 4.
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