CN106491374B - Nepetalactone microcapsule, preparation method thereof and application of nepetalactone microcapsule in preparation of mosquito repellent product - Google Patents
Nepetalactone microcapsule, preparation method thereof and application of nepetalactone microcapsule in preparation of mosquito repellent product Download PDFInfo
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Abstract
The invention relates to the technical field of daily chemicals, in particular to a nepetalactone microcapsule, a preparation method thereof and application thereof in preparing mosquito repellent products. The invention provides a microcapsule of nepetalactone, which takes nepetalactone adsorbed by porous starch as a core material and is embedded by gelatin, and the formed microcapsule is stable and has good slow release performance, thereby reducing volatilization of nepetalactone and prolonging mosquito avoidance time and avoidance rate. Moreover, the humulus scandens extract, the viola yedoensis extract and the rose hydrosol in the mosquito repelling gel provided by the invention also have the effects of anti-allergy, anti-inflammation, itching relieving and swelling diminishing, reduce the irritation to the skin, and have better safety and more efficient mosquito repelling effect. Experiments show that under a proper proportion, the effective mosquito repelling time of the mosquito repelling gel provided by the invention can reach 13.7 h; the effective avoidance rate can still reach 84.6 percent after 18 hours.
Description
Technical Field
The invention relates to the technical field of daily chemicals, in particular to a nepetalactone microcapsule, a preparation method thereof and application thereof in preparing mosquito repellent products.
Background
Besides direct stinging and harassment, the main harm of mosquitoes to human beings lies in the transmission of various diseases. Mosquitoes have received great attention as important disease vectors since the beginning of the last century. The insect-borne virus diseases such as yellow fever, dengue fever, trogopterus fever, eastern equine encephalitis, western equine encephalitis, venezuelan equine encephalitis, epidemic encephalitis B, Ross river fever and the like are successively proved to be transmitted by mosquitoes. At present, in mosquito products, because of using a large amount of chemical insecticides, the problems of drug resistance, environmental pollution and the like are caused, and more importantly, the chemical components are re-absorbed by other human bodies through skin, so that the health of human is seriously harmed. In recent years, with the development of society and the increasing improvement of human life quality, it is important to find a natural mosquito repellent product with small toxic and side effect, safety, environmental protection and easy degradation for insect prevention and killing. Plant-derived repellents have the potential to become a new generation of mosquito repellent.
The plant-derived repellent has low toxic and side effects to human body because the active ingredients are mainly from natural plants. The plant volatile oil with mosquito repellent activity is mostly terpenoid ester, alcohol, ketone compound, etc. Other components such as alkaloid, flavonoid, etc. are also available. Schizonepeta, also known as catnip. Is an annual herbaceous plant of schizonepeta of Labiatae, has warm and pungent properties, has the efficacies of dispelling wind, relieving exterior syndrome, dispersing toxin, promoting eruption, dissipating blood stasis and stopping bleeding, has the efficacies of mainly treating wind-cold type cold, sore throat, various skin diseases and the like, has long application history, and is one of the traditional large traditional Chinese medicinal materials. The nepetalactone is an active mosquito repellent component extracted and separated from catnip, the repellent effect of the nepetalactone is obviously better than that of deet, and a comparison test shows that the concentration of the deet reaches 468mg/cm-2The protection time can be 6h, while the content of nepetalactone is only 23mg/cm-2. However, its use is greatly limited due to its strong volatility.
In order to solve the problem of short effect caused by strong volatility of the nepetalactone, so that the nepetalactone can play a long-acting mosquito repellent effect, a sustained-release formulation of the nepetalactone should be further developed.
Disclosure of Invention
In view of the above, the technical problem to be solved by the invention is to provide a nepetalactone microcapsule, a preparation method thereof and an application thereof in preparing a mosquito repellent product.
The invention provides a method for preparing nepetalactone microcapsules from the following raw materials in parts by mass:
40-80 parts of gelatin;
40-80 parts of porous starch;
4 to 10 portions of nepetalactone.
The porous starch granule surface presents porous starch granule, has larger specific pore volume, larger specific surface area and good water absorption and oil absorption capacity, can keep obvious structural integrity in solvent, can absorb all other substances except paste substances, is safe and nontoxic, is an ideal microcapsule core material and adsorbent, has low cost and can be naturally degraded. Gelatin is a protein that is partially hydrolyzed from collagen in the connective or epidermal tissues of animals. The gelatin contains water and inorganic salt about 16%, and protein over 82%. The invention uses porous starch to adsorb nepetalactone as a core material, and then uses gelatin to carry out embedding, thus forming a double-layer embedding system. The formed microcapsule is stable and has good slow release performance, thereby reducing volatilization of the nepetalactone and prolonging the mosquito repelling time and the mosquito repelling rate.
In some embodiments, the mass ratio of gelatin, porous starch, and nepetalactone is 40:80: 4.
In other embodiments, the mass ratio of gelatin, porous starch, and nepetalactone is 60:60: 10.
In other examples, the mass ratio of gelatin, porous starch, and nepetalactone is 80:40: 7.
In other examples, the mass ratio of gelatin, porous starch, and nepetalactone is 50:80: 6.
The invention provides a preparation method of nepetalactone microcapsules, which comprises the following steps:
step 1: dissolving gelatin in water, heating to 70-80 ℃, and stirring to obtain a gelatin solution;
dissolving porous starch in water, heating to 70-80 ℃, and stirring to obtain a porous starch solution;
dissolving nepetalactone in absolute ethanol to obtain nepetalactone solution;
step 2: dropwise adding the nepetalactone solution into the porous starch solution, stirring for 1-2.5 h, adding the gelatin solution, and then continuously stirring for 1.5-3 h to prepare a coarse emulsion;
and step 3: spray drying the crude emulsion to obtain nepetalactone microcapsules.
In the embodiment of the invention, in the step 1,
the mass volume ratio of the gelatin to the water is (40 g-80 g): (200 mL-400 mL);
the mass volume ratio of the porous starch to the water is (40 g-80 g): (200 mL-400 mL);
the mass volume ratio of the nepetalactone to the absolute ethyl alcohol is (4 g-10 g): (50 mL-100 mL).
In the embodiment of the present invention, the spray drying conditions in step 3 are as follows: the feeding flow is 60 ml/min-80 ml/min, and the drying air flow is 70m3/h～80m3The temperature of the hot air inlet is 180-200 ℃, and the temperature of the hot air outlet is 60-70 ℃.
The nepetalactone microcapsule prepared by the method provided by the invention has uniform particles and higher encapsulation efficiency and loading capacity on nepetalactone, thereby ensuring that nepetalactone can be slowly released and achieving the long-acting repelling effect on mosquitoes.
The invention provides application of nepetalactone microcapsules in preparation of mosquito repellent products.
The mosquito repelling product is mosquito repelling liquid, mosquito repelling emulsion, mosquito repelling gel, mosquito repelling spray, mosquito repelling liquid, mosquito repelling tablet, mosquito repelling bracelet or mosquito repelling paste.
The invention also provides a mosquito repellent product, which comprises the following components in percentage by mass:
the mosquito repelling product provided by the invention is mosquito repelling gel.
In the mosquito-repellent product provided by the invention,
the thickening agent is selected from carbomer, seaweed gum, acacia gum, xanthan gum or Caesalpinia spinosa;
the humectant is selected from glycerin, propylene glycol, butylene glycol, hexylene glycol, sodium hyaluronate, beta-glucan, allantoin or nicotinamide;
the pH regulator is selected from triethanolamine or citric acid.
In one specific embodiment, the mosquito repelling product provided by the invention comprises the following components in parts by mass:
30% of the nepetalactone microcapsule, 8% of a humulus scandens extract, 3% of a Chinese violet extract, 1% of rose hydrosol, 3% of glycerin, 2.5% of butanediol, 0.5% of beta-glucan, 0.3% of carbomer, 0.2% of xanthan gum, 0.3% of triethanolamine and deionized water to make up 100%.
In one specific embodiment, the mosquito repelling product provided by the invention comprises the following components in parts by mass:
15% of the nepetalactone microcapsule, 2% of a humulus scandens extract, 10% of a Chinese violet extract, 0.5% of rose hydrosol, 7.7% of propylene glycol, 0.5% of hyaluronic acid, 0.8% of hexanediol, 0.6% of seaweed gel, 0.4% of Caesalpinia spinosa gel, 0.2% of triethanolamine, 0.3% of citric acid and deionized water to make up 100%.
In one specific embodiment, the mosquito repelling product provided by the invention comprises the following components in parts by mass:
22.5 percent of the nepetalactone microcapsule, 5 percent of humulus scandens extract, 6.5 percent of Chinese violet extract, 0.75 percent of rose hydrosol, 11 percent of glycerin, 0.8 percent of nicotinamide, 0.2 percent of allantoin, 0.4 percent of acacia gum and deionized water to make up 100 percent.
In one specific embodiment, the mosquito repelling product provided by the invention comprises the following components in parts by mass:
20% of the nepetalactone, 4% of a scandent hop extract, 7% of a Chinese violet extract, 0.6% of rose hydrosol, 7% of glycerin, 0.7% of Caesalpinia spinosa gum, 0.2% of citric acid and deionized water to make up 100%.
The preparation method of the mosquito repelling gel provided by the invention comprises the following steps: dissolving a thickening agent in deionized water, swelling, mixing with the nepetalactone microcapsules and the humectant, adjusting the pH value to 5-7, and adding the humulus scandens extract, the philippine violet herb extract and the rose hydrosol to prepare the mosquito repellent gel.
In a specific embodiment, the swelling temperature is 70-80 ℃; the swelling condition is homogeneous.
In the specific embodiment, the swelling is carried out, the temperature is cooled to 40-45 ℃, and the nepetalactone microcapsules and the humectant are added.
The invention provides a microcapsule of nepetalactone, which takes nepetalactone adsorbed by porous starch as a core material and is embedded by gelatin, and the formed microcapsule is stable and has good slow release performance, thereby reducing volatilization of nepetalactone and prolonging mosquito avoidance time and avoidance rate. Moreover, the humulus scandens extract, the viola yedoensis extract and the rose hydrosol in the mosquito repelling gel provided by the invention also have the effects of anti-allergy, anti-inflammation, itching relieving and swelling diminishing, reduce the irritation to the skin, and have better safety and more efficient mosquito repelling effect. Experiments show that under a proper proportion, the effective mosquito repelling time of the mosquito repelling gel provided by the invention can reach 13.7 h; the effective avoidance rate can still reach 84.6 percent after 18 hours.
Detailed Description
The invention provides a nepetalactone microcapsule, a preparation method thereof and application thereof in preparing mosquito repellent products. It is expressly intended that all such similar substitutes and modifications which would be obvious to one skilled in the art are deemed to be included in the invention. While the methods and applications of this invention have been described in terms of preferred embodiments, it will be apparent to those of ordinary skill in the art that variations and modifications in the methods and applications described herein, as well as other suitable variations and combinations, may be made to implement and use the techniques of this invention without departing from the spirit and scope of the invention.
The test materials adopted by the invention are all common commercial products and can be purchased in the market.
Wherein the humulus scandens extract is purchased from Saaney biotechnology, Inc.;
the extract of Viola yedoensis Makino is purchased from Siemens Biotech, Inc.;
rose Benth Hibiscus was purchased from Hibiscus scientific development, Guangzhou, Han Cubs.
The invention is further illustrated by the following examples:
example 1
Dissolving 40g of gelatin in 200mL of deionized water in advance, dissolving 80g of porous starch in 400mL of deionized water, and heating and stirring at 70 ℃ until the gelatin is clear and transparent; dissolving 4g of nepetalactone in 50mL of absolute ethanol, preheating, then dropwise adding the solution into the porous starch solution, continuously stirring for 1h, then adding a gelatin solution, continuously stirring for 2h, and finally obtaining the coarse emulsion containing the nepetalactone microcapsules. Spray drying the emulsion under the conditions of: the feed flow rate was 60ml/min, and the dry air flow rate was 75m3The inlet temperature of hot air is 200 ℃ and the outlet temperature is 60 ℃.
Example 2
Dissolving 60g of gelatin in 300mL of deionized water in advance, dissolving 60g of porous starch in 300mL of deionized water, and heating and stirring at 80 ℃ until the gelatin is clear and transparent; dissolving 10g of nepetalactone in 100mL of absolute ethanol, preheating, then dropwise adding the solution into the porous starch solution, continuously stirring for 2h, then adding a gelatin solution, continuously stirring for 1.5h, and finally obtaining the coarse emulsion containing the nepetalactone microcapsules. Spray drying the emulsion under the conditions of: the feed flow rate was 70ml/min, and the dry air flow rate was 70m3The hot air inlet temperature is 190 ℃ and the hot air outlet temperature is 65 ℃.
Example 3
Dissolving 80g of gelatin in 400mL of deionized water in advance, dissolving 40g of porous starch in 200mL of deionized water, and heating and stirring at 75 ℃ until the gelatin is clear and transparent; dissolving 7g of nepetalactone in 75mL of absolute ethanol, preheating, and then dissolving the solutionDropwise adding into the porous starch solution, continuously stirring for 2.5h, adding gelatin solution, and stirring for 3h to obtain coarse emulsion containing nepetalactone microcapsule. Spray drying the emulsion under the conditions of: the feed flow was 70ml/min and the dry air flow was 80m3The hot air inlet temperature is 180 ℃ and the hot air outlet temperature is 70 ℃.
Example 4
Dissolving 50g of gelatin in 250mL of deionized water in advance, dissolving 80g of porous starch in 400mL of deionized water, and heating and stirring at 70 ℃ until the gelatin is clear and transparent; taking 6g of nepetalactone, dissolving in 55mL of absolute ethanol, preheating, then dropwise adding the solution into the porous starch solution, continuously stirring for 2h, then adding a gelatin solution, continuously stirring for 2.5h, and finally obtaining the coarse emulsion containing the nepetalactone microcapsules. Spray drying the emulsion under the conditions of: the feed flow was 70ml/min and the dry air flow was 75m3The hot air inlet temperature is 190 ℃ and the hot air outlet temperature is 60 ℃.
Example 5
Dissolving 120g of gelatin in 600mL of deionized water in advance, dissolving 60g of porous starch in 300mL of deionized water, and heating and stirring at 70 ℃ until the gelatin is clear and transparent; dissolving 4g of nepetalactone in 50mL of absolute ethanol, preheating, then dropwise adding the solution into the porous starch solution, continuously stirring for 1h, then adding a gelatin solution, continuously stirring for 2h, and finally obtaining the coarse emulsion containing the nepetalactone microcapsules. Spray drying the emulsion under the conditions of: the feed flow rate was 60ml/min, and the dry air flow rate was 75m3The inlet temperature of hot air is 200 ℃ and the outlet temperature is 60 ℃.
Example 6
Dissolving 10g of gelatin in 50mL of deionized water in advance, dissolving 15g of porous starch in 75mL of deionized water, and heating and stirring at 80 ℃ until the gelatin is clear and transparent; dissolving 10g of nepetalactone in 100mL of absolute ethanol, preheating, then dropwise adding the solution into the porous starch solution, continuously stirring for 2h, then adding a gelatin solution, continuously stirring for 1.5h, and finally obtaining the coarse emulsion containing the nepetalactone microcapsules. Subjecting the above emulsion toSpray drying under the conditions: the feed flow rate was 70ml/min, and the dry air flow rate was 70m3The hot air inlet temperature is 190 ℃ and the hot air outlet temperature is 65 ℃.
Example 7
30g of the nepetalactone microcapsules of example 1, 8g of the humulus scandens extract, 3g of the viola yedoensis extract, 1g of the rose hydrosol, 3g of the glycerol, 2.5g of the butanediol, 0.5g of the beta-glucan, 0.3g of the carbomer, 0.2g of the xanthan gum, 0.3g of the triethanolamine and the balance of the deionized water to 100 g.
The preparation method comprises the following steps:
adding a thickening agent into a proper amount of deionized water, heating to 70-80 ℃, and homogenizing to fully swell; cooling to 40-45 ℃, adding the humectant and the nepetalactone microcapsules, and uniformly stirring; slowly dripping a pH regulator to regulate the pH to 5-7; finally, adding the humulus scandens extract, the Chinese violet extract and the rose hydrosol, and uniformly stirring; and (4) supplementing the rest deionized water, and uniformly stirring to obtain the mosquito repellent gel.
Example 8
15g of the nepetalactone microcapsules of example 2, 2g of the scandent hop extract, 10g of the Chinese violet extract, 0.5g of the rose hydrosol, 7.7g of propylene glycol, 0.5g of hyaluronic acid, 0.8g of hexanediol, 0.6g of seaweed gel, 0.4g of Caesalpinia spinosa gel, 0.2g of triethanolamine, 0.3g of citric acid and the balance of deionized water to 100 g.
The preparation method comprises the following steps:
adding a thickening agent into a proper amount of deionized water, heating to 70-80 ℃, and homogenizing to fully swell; cooling to 40-45 ℃, adding the humectant and the nepetalactone microcapsules, and uniformly stirring; slowly dripping a pH regulator to regulate the pH to 5-7; finally, adding the humulus scandens extract, the Chinese violet extract and the rose hydrosol, and uniformly stirring; and (4) supplementing the rest deionized water, and uniformly stirring to obtain the mosquito repellent gel.
Example 9
22.5g of the nepetalactone microcapsules of example 3, 5g of the humulus scandens extract, 6.5g of the Chinese violet extract, 0.75g of the rose hydrosol, 11g of the glycerin, 0.8g of the niacinamide, 0.2g of the allantoin, 0.4g of the acacia gum and the balance of deionized water to 100 g.
The preparation method comprises the following steps:
adding a thickening agent into a proper amount of deionized water, heating to 70-80 ℃, and homogenizing to fully swell; cooling to 40-45 ℃, adding the humectant and the nepetalactone microcapsules, and uniformly stirring; slowly dripping a pH regulator to regulate the pH to 5-7; finally, adding the humulus scandens extract, the Chinese violet extract and the rose hydrosol, and uniformly stirring; and (4) supplementing the rest deionized water, and uniformly stirring to obtain the mosquito repellent gel.
Example 10
20g of nepetalactone of example 4, 4g of scandent hop extract, 7g of Chinese violet extract, 0.6g of rose hydrosol, 7g of glycerin, 0.7g of Caesalpinia spinosa gum, 0.2g of citric acid and deionized water to make up 100 g.
The preparation method comprises the following steps:
adding a thickening agent into a proper amount of deionized water, heating to 70-80 ℃, and homogenizing to fully swell; cooling to 40-45 ℃, adding the humectant and the nepetalactone microcapsules, and uniformly stirring; slowly dripping a pH regulator to regulate the pH to 5-7; finally, adding the humulus scandens extract, the Chinese violet extract and the rose hydrosol, and uniformly stirring; and (4) supplementing the rest deionized water, and uniformly stirring to obtain the mosquito repellent gel.
Example 11
30g of the nepetalactone microcapsules of example 5, 8g of the humulus scandens extract, 3g of the viola yedoensis extract, 1g of the rose hydrosol, 3g of the glycerol, 2.5g of the butanediol, 0.5g of the beta-glucan, 0.3g of the carbomer, 0.2g of the xanthan gum, 0.3g of the triethanolamine and the balance of the deionized water to 100 g.
The preparation method comprises the following steps:
adding a thickening agent into a proper amount of deionized water, heating to 70-80 ℃, and homogenizing to fully swell; cooling to 40-45 ℃, adding the humectant and the nepetalactone microcapsules, and uniformly stirring; slowly dripping a pH regulator to regulate the pH to 5-7; finally, adding the humulus scandens extract, the Chinese violet extract and the rose hydrosol, and uniformly stirring; and (4) supplementing the rest deionized water, and uniformly stirring to obtain the mosquito repellent gel.
Example 12
15g of the nepetalactone microcapsules of example 6, 2g of the scandent hop extract, 10g of the Chinese violet extract, 0.5g of the rose hydrosol, 7.7g of the propylene glycol, 0.5g of the hyaluronic acid, 0.8g of the hexanediol, 0.6g of the seaweed gel, 0.4g of the Caesalpinia spinosa gel, 0.2g of the triethanolamine, 0.3g of the citric acid and the balance of the deionized water to 100 g.
The preparation method comprises the following steps:
adding a thickening agent into a proper amount of deionized water, heating to 70-80 ℃, and homogenizing to fully swell; cooling to 40-45 ℃, adding the humectant and the nepetalactone microcapsules, and uniformly stirring; slowly dripping a pH regulator to regulate the pH to 5-7; finally, adding the humulus scandens extract, the Chinese violet extract and the rose hydrosol, and uniformly stirring; and (4) supplementing the rest deionized water, and uniformly stirring to obtain the mosquito repellent gel.
Example 13
Mosquito repelling test conditions are as follows: species and strains of test mosquitoes: aedes albopictus (Aedes albopictus), Culex fatigues (Culex pipiens quinquefasciatus) and Culex pallens (Culex pipiens); animals for the test: SD rat; test temperature: 26 +/-1 ℃; relative humidity: 65 plus or minus 5 percent;
test materials: the mosquito repellent gels of examples 7-12, further comprising Liushen mosquito repellent toilet water and Longliki mosquito repellent toilet water as repellent groups and deionized water as blank groups.
100 test insects were placed in a mosquito cage of 20cm × 20cm × 20cm, and hairs of 2cm × 2cm area were cut from the abdominal area of the rat to expose the skin. After being fixed, the rat is placed in a mosquito cage for 5min, more than 20 test insects which come and stop and fall are qualified in the attack power, and the mosquitoes in the rat and the cage can be used for the test. Fixing SD rat qualified in the attack force test, shearing to expose 2cm × 2cm skin on abdomen, and processing at a speed of 1.5mg/cm2(gel) or 1.5. mu.L/cm2(control floral water) the test sample and the control sample are uniformly smeared, the rest parts are tightly covered, the rat is placed in a mosquito cage with qualified attack force for 5min after the drug is smeared for 1h, whether the mosquitoes are stopped and fall on the exposed skin is observed, the rat is tested once per hour, and the rat is judged to be invalid as long as 1 mosquito is stopped and falls. And repeating the test for 3 times, recording the average effective mosquito repelling time of each sample, and calculating the effective mosquito repelling rate.
The effective mosquito repelling rate is (corresponding to the average number of the stops of the blank preparation-the average number of the stops of the repellent)/the average number of the stops of the corresponding blank preparation is multiplied by 100 percent
TABLE 1 average effective mosquito repellent time (h) for several repellents
| Aedes albopictus | Culex fatigues | Culex pipiens pallens | |
| Mosquito repellent gel of example 7 | 13.5*# | 11.2*# | 12.7*# |
| Mosquito repellent gel of example 8 | 13.1*# | 11.0*# | 11.9*# |
| Mosquito repellent gel of example 9 | 13.7*# | 11.5*# | 12.4*# |
| Mosquito repellent gel of example 10 | 13.4*# | 11.3*# | 12.3*# |
| Example 11 mosquito repellent gelGlue | 9.3# | 7.8# | 8.6# |
| Mosquito repellent gel of example 12 | 8.8# | 7.0# | 7.9# |
| Liushen mosquito-repellent toilet water | 5.8 | 6.1 | 6.7 |
| Longliki mosquito-repellent toilet water | 5.3 | 6.5 | 7 |
Note that # shows that compared with the effect of Liushen mosquito repellent toilet water or Longli mosquito repellent toilet water, the effect is significantly different, and p is less than 0.05;
showing that there is a significant difference compared to the effect of the mosquito repellent gel of example 11 or the mosquito repellent gel of example 12, p is < 0.05.
TABLE 2 effective repellency Rate (%) of several repellents
The results show that the effective mosquito repelling time and the effective repellent rate of the mosquito repelling gel on aedes albopictus, culex fatigues and culex pallens are obviously higher than those of Liushen mosquito repelling toilet water and Longli mosquito repelling toilet water, but the improper formula can weaken the mosquito repelling effect.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that it is obvious to those skilled in the art that various modifications and improvements can be made without departing from the principle of the present invention, and these modifications and improvements should also be considered as the protection scope of the present invention.
Claims (9)
1. A nepetalactone microcapsule is characterized by being prepared from gelatin, porous starch and nepetalactone, wherein the mass ratio of the gelatin to the porous starch to the nepetalactone is 40:80:4 or 60:60:10 or 80:40:7 or 50:80: 6.
2. A process for the preparation of nepetalactone microcapsules of claim 1, comprising:
step 1: dissolving gelatin in water, heating to 70-80 ℃, and stirring to obtain a gelatin solution;
dissolving porous starch in water, heating to 70-80 ℃, and stirring to obtain a porous starch solution;
dissolving nepetalactone in absolute ethanol to obtain nepetalactone solution;
step 2: dropwise adding the nepetalactone solution into the porous starch solution, stirring for 1-2.5 h, adding the gelatin solution, and then continuously stirring for 1.5-3 h to prepare a coarse emulsion;
and step 3: spray drying the crude emulsion to obtain nepetalactone microcapsules.
3. The production method according to claim 2, wherein, in step 1,
the mass volume ratio of the gelatin to the water is (40 g-80 g): (200 mL-400 mL);
the mass volume ratio of the porous starch to the water is (40 g-80 g): (200 mL-400 mL);
the mass volume ratio of the nepetalactone to the absolute ethyl alcohol is (4 g-10 g): (50 mL-100 mL).
4. Root of herbaceous plantThe method according to claim 2, wherein the spray-drying conditions in step 3 are as follows: the feeding flow is 60 ml/min-80 ml/min, and the drying air flow is 70m3/h～80m3The temperature of the hot air inlet is 180-200 ℃, and the temperature of the hot air outlet is 60-70 ℃.
5. Use of the nepetalactone microcapsules of claim 1 in the manufacture of a mosquito repellent product.
6. The mosquito repelling gel is characterized by comprising the following components in percentage by mass:
7. a mosquito repellent gel according to claim 6,
the thickening agent is selected from carbomer, seaweed gum, acacia gum, xanthan gum or Caesalpinia spinosa;
the humectant is selected from glycerin, propylene glycol, butylene glycol, hexylene glycol, sodium hyaluronate, beta-glucan, allantoin or nicotinamide;
the pH regulator is selected from triethanolamine or citric acid.
8. A method for preparing mosquito repellent gel according to any one of claims 6 to 7, wherein the mosquito repellent gel is prepared by dissolving thickener in deionized water, swelling, mixing with the nepetalactone microcapsules of claim 1 and humectant, adjusting pH to 5-7, and adding humulus scandens extract, herba Violae extract and flos Rosae Rugosae hydrosol.
9. The method according to claim 8, wherein the swelling temperature is 70 ℃ to 80 ℃; the swelling condition is homogeneous.
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