CN106430831A - Bio-microcapsule-ultrafiltration membrane combined process for treating heavy metals and ammonia nitrogen in water - Google Patents
Bio-microcapsule-ultrafiltration membrane combined process for treating heavy metals and ammonia nitrogen in water Download PDFInfo
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- CN106430831A CN106430831A CN201610963612.XA CN201610963612A CN106430831A CN 106430831 A CN106430831 A CN 106430831A CN 201610963612 A CN201610963612 A CN 201610963612A CN 106430831 A CN106430831 A CN 106430831A
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- bio
- microcapsule
- water
- solution
- ammonia nitrogen
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Classifications
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F9/00—Multistage treatment of water, waste water or sewage
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/28—Treatment of water, waste water, or sewage by sorption
- C02F1/286—Treatment of water, waste water, or sewage by sorption using natural organic sorbents or derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/44—Treatment of water, waste water, or sewage by dialysis, osmosis or reverse osmosis
- C02F1/444—Treatment of water, waste water, or sewage by dialysis, osmosis or reverse osmosis by ultrafiltration or microfiltration
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/02—Aerobic processes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- Life Sciences & Earth Sciences (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
A bio-microcapsule-ultrafiltration membrane combined process for treating heavy metals and ammonia nitrogen in water comprises steps as follows: (1) preparation of bio-microcapsules: following operation is performed sequentially under the sterile condition: a saccharomyces cerevisiae strain culture solution in the logarithmic phase is taken, and a strain is extracted; the strain solution is added to a sodium alginate solution and dropwise added to a calcium chloride solution for curing; colloidal beads obtained through the operation are placed in a chitosan solution for laminating, and the volume ratio of the calcium alginate colloidal beads to the chitosan solution is 1:5; the colloidal beads are placed in a glutaraldehyde solution for crosslinking after being fished out, finally, milk white colloidal balls which are round and uniform and have good mechanical strength, permeability and biocompatibility are prepared, and the bio-microcapsules are prepared and put in normal saline for later use; (2) the bio-microcapsules prepared in the step (1) for later use are put in a combined water treatment system for process treatment. Used saccharomyces cerevisiae widely exist in the natural world, is commonly applied in the fermenting industry, is cheap and easy to obtain and is a preferred material of a bio-adsorbent.
Description
Technical field
The invention belongs to water treatment field is and in particular to a kind of micro- glue of biology for processing heavy metal and ammonia nitrogen in water
Capsule-milipore filter combination water treatment technology.
Background technology
Conventional water treatment technology to the heavy metal in micropollutant water, ammonia nitrogen and organic pollutant removal effect on driving birds is not good,
At present in numerous water technologies, biologic treating technique, because of high treating effect, is particularly less than the molten of 1500Da to molecular weight
Solution property organic matter removal effect is significant, non-secondary pollution and operating cost relatively low it is considered to be the most promising process skill
One of art.
Bio-microcapsule technology, for traditional biological method, can utilize absorption and biodegradable process water body simultaneously
In ammonia nitrogen and organic matter, efficiency high；Throw and use, greatly save bio-film colonization required time in traditional biological pretreatment；
And microorganism, by after microcapsule embedded, can be effective against the fluctuation of external environment, breach the limitation of traditional biological technology
Property.The combination of membrane technology and other techniques is always study hotspot, due to colloid in membrane technology energy trap water, solid suspension with
And major part virus, bacterium, but not satisfactory to ammonia nitrogen and dissolved organic matter removal effect, and biologic treating technique often has
The hidden danger of biological leakage.
Content of the invention
Present invention aim to overcome that the deficiencies in the prior art, the technical problem to be solved is for existing normal
To the heavy metal of micro-polluted raw and the problem of ammonia nitrogen removal effect on driving birds is not good in rule water treatment technology, provide a kind of biological micro- glue
Capsule-milipore filter combination water treatment technology.The present invention is combined bio-microcapsule-ultrafiltration membrane process, can effectively remove heavy metal in eliminating water
And ammonia nitrogen, and solve the hidden danger of biological leakage.
Technical solution of the present invention is：
A kind of bio-microcapsule of heavy metal and ammonia nitrogen in process water-milipore filter combination water treatment technology it is characterised in that
Carry out as follows：
(1) preparation of bio-microcapsule：
Aseptically operate as follows successively：
Take the saccharomyces cerevisiae bacterial classification nutrient solution being in exponential phase, extract bacterial classification；
Strain liquid adds in sodium alginate soln, and instills in calcium chloride solution, solidification；
Aforesaid operations gained glue bead is placed in overlay film in chitosan solution, wherein calcium alginate plastic beads and chitosan solution body
Long-pending ratio is 1:5；
Be placed in glutaraldehyde solution crosslinked after pulling out, be finally obtained mellow and full uniformly, have good mechanical strength, permeability and
The bio-microcapsule of the milky glueballs of biocompatibility, as present invention preparation, is placed in standby in physiological saline；
(2) the combination water treatment system that bio-microcapsule standby for step (1) is put into the present invention carries out PROCESS FOR TREATMENT：
Described combination water treatment system structural characterization is：Including reactor and filter, both are connected by pipeline；Institute
State reactor bottom and be provided with aeration tube 2, whole container is biological contact reaction area 1, adds pending raw water by inlet pipeline,
It is used for putting into standby bio-microcapsule by lid structure, the export department of outlet pipe 4 is provided with downflow weir 3, upper located at reactor
Portion；It is provided with some hyperfiltration membrane assemblies 5 in described filter, receive and pass through hyperfiltration membrane assembly 5 after the process water of reactor output,
Flow out again；
PROCESS FOR TREATMENT：Pending raw water enters equipped with the biological contact reaction area 1 of bio-microcapsule first, in aeration tube
It is fully contacted reaction with water body, water body enters downflow weir 3, warp from outlet pipe 4 after purification after a while in the presence of 2
Cross water outlet (as shown in Figure 1) after hyperfiltration membrane assembly 5 effect.
Compared with prior art, beneficial effect of the present invention：
S. cervisiae used in the present invention is widespread in nature, and applies universal, inexpensively in fermentation industry
It is easy to get, be the preferred material of biological adsorption agent.Compared with conventional adsorbent, yeast bacteria adsorbent low cost, clearance is high, especially
It is especially effective when processing the heavy metal ion aqueous solution, and be easily isolated recovery metal, there is higher power of regeneration.This
Invention is used in combination bio-microcapsule-ultrafiltration membrane technique, reaches complementary effect.
Brief description
Fig. 1：Embodiment 1 bio-microcapsule-milipore filter process schematic representation.
Fig. 2：Embodiment 2 heavy metal treatment effect figure.
Specific embodiment
Below in conjunction with drawings and Examples, technical solution of the present invention is described further.
Embodiment 1
A kind of bio-microcapsule of heavy metal and ammonia nitrogen in process water-milipore filter combination water treatment technology it is characterised in that
Carry out as follows：
(1) preparation of bio-microcapsule：Take the saccharomyces cerevisiae bacterial classification nutrient solution 20mL being in exponential phase, put into drying
In 4000rpm in refrigerated centrifuge, under the conditions of 15 DEG C, it is centrifuged 5min.Remove supernatant, with brine 3 times, final use
10mL physiological saline suspends and extracts bacterial classification, and on turbula shaker, vibration makes to mix, and makes bacterial classification suspension, its
OD600 value is 1.5 about.Strain liquid adds 3% sodium alginate, uniformly mixes, and is mixed with the bacterial classification that syringe draws certain volume
Close liquid and at the uniform velocity instill 4% calcium chloride solution, solidify 30min；Gained glue bead is placed in overlay film 10min in 1.8% chitosan solution,
Wherein calcium alginate plastic beads and chitosan solution volume ratio are 1:5, it is placed in 1% glutaraldehyde solution crosslinked 5min after pulling out, whole
Individual range request sterile working excessively.Finally it is obtained mellow and full uniform, there is the milky white of good mechanical strength, permeability and biocompatibility
Coloring agent ball, is placed in standby in physiological saline.
(2) pending raw water enters equipped with the biological contact reaction area 1 of bio-microcapsule first, in the work of aeration tube 2
It is fully contacted reaction with lower with water body, water body enters downflow weir 3 after purification after a while, through super from outlet pipe 4
Water outlet (as shown in Figure 1) after filter membrane component 5 effect.
Combinations thereof system has the advantage that：
(1) Blastocystis, in emerging Biosorbent for heavy metals, has splendid adsorption and enrichment energy to various heavy
Power.Also there is good degradation effect to ammonia nitrogen, it has the characteristics that filamentous fungi simultaneously, metabolism is vigorous, the removal speed to COD
Hurry up, acidproof, osmophilic strain, resisting high-concentration organic substrates.The new bio carrier organism microcapsules energy of present invention preparation
Provide a kind of small Liquid Culture environment for intracapsular thalline, reduce the interference to growth of microorganism for the external environment condition simultaneously,
Maintain the normal growth of microorganism, improve microbe density.And microencapsulated forms are easy to observe, control and reclaim, improve it
Practical application.
(2) ultrafiltration membrane technique can solve the biological hidden danger revealed of microcapsules technology, and in energy trap water, colloid, solid suspend
Thing and most of virus, bacterium.
Embodiment 2
Compliance test result to embodiment 1
Influent turbidity 126NTU, ammonia nitrogen 1.51mg/L, Pb (II) 0.1mg/L, Cr (VI) 0.1mg/L, micro- through this biology
After capsule-milipore filter combined system, water outlet ammonia nitrogen removal frank maintains 80%, Pb (II) clearance more than 90%, Cr (VI)
Clearance reaches 60%.Effluent quality is better than former conventional water treatment process, particularly substantially improves process system heavy metal
Treatment effect (as shown in Figure 2).
Claims (1)
1. a kind of bio-microcapsule-milipore filter combination water treatment technology processing heavy metal and ammonia nitrogen in water is it is characterised in that press
Following steps are carried out：
(1) preparation of bio-microcapsule：
Aseptically operate as follows successively：
Take the saccharomyces cerevisiae bacterial classification nutrient solution being in exponential phase, extract bacterial classification；
Strain liquid adds in sodium alginate soln, and instills in calcium chloride solution, solidification；
Aforesaid operations gained glue bead is placed in overlay film in chitosan solution, wherein calcium alginate plastic beads and chitosan solution volume ratio
For 1:5；
It is placed in glutaraldehyde solution crosslinked after pulling out, finally bio-microcapsule is obtained, is placed in standby in physiological saline；
(2) the combination water treatment system that bio-microcapsule standby for step (1) is put into the present invention carries out PROCESS FOR TREATMENT：
Described combination water treatment system structural characterization is：Including reactor and filter, both are connected by pipeline；Described anti-
Device bottom is answered to be provided with aeration tube 2, whole container is biological contact reaction area 1, adds pending raw water by inlet pipeline, passes through
Lid structure is used for putting into standby bio-microcapsule, and the export department of outlet pipe 4 is provided with downflow weir 3, located at the top of reactor；Institute
State and in filter, be provided with some hyperfiltration membrane assemblies 5, receive and pass through hyperfiltration membrane assembly 5 after the process water of reactor output, then flow
Go out；
PROCESS FOR TREATMENT：Pending raw water enters equipped with the biological contact reaction area 1 of bio-microcapsule first, in aeration tube 2
Effect is lower and water body is fully contacted reaction, and water body enters downflow weir 3 after purification after a while, passes through from outlet pipe 4
Water outlet after hyperfiltration membrane assembly 5 effect.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610963612.XA CN106430831A (en) | 2016-10-28 | 2016-10-28 | Bio-microcapsule-ultrafiltration membrane combined process for treating heavy metals and ammonia nitrogen in water |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610963612.XA CN106430831A (en) | 2016-10-28 | 2016-10-28 | Bio-microcapsule-ultrafiltration membrane combined process for treating heavy metals and ammonia nitrogen in water |
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| CN106430831A true CN106430831A (en) | 2017-02-22 |
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| CN201610963612.XA Pending CN106430831A (en) | 2016-10-28 | 2016-10-28 | Bio-microcapsule-ultrafiltration membrane combined process for treating heavy metals and ammonia nitrogen in water |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110818092A (en) * | 2018-08-09 | 2020-02-21 | 贺特佳研究私人有限公司 | Chitosan coated calcium alginate bacterial beads for ammonia removal in treatment of wastewater with bioaugmentation |
| CN114767923A (en) * | 2022-03-24 | 2022-07-22 | 金发科技股份有限公司 | Composite hydrogel silver-loaded long-acting antibacterial dressing and preparation method and application thereof |
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| CN102181507A (en) * | 2011-03-02 | 2011-09-14 | 江门量子高科生物股份有限公司 | Preparation method of galactooligosaccharides |
| CN102351320A (en) * | 2011-07-01 | 2012-02-15 | 李朝霞 | Method for preparing novel biological microcapsule for biological fluidized bed |
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Cited By (4)
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|---|---|---|---|---|
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| CN110818092B (en) * | 2018-08-09 | 2022-10-21 | 贺特佳研究私人有限公司 | Chitosan coated calcium alginate bacterial beads for ammonia removal in treatment of wastewater with bioaugmentation |
| CN114767923A (en) * | 2022-03-24 | 2022-07-22 | 金发科技股份有限公司 | Composite hydrogel silver-loaded long-acting antibacterial dressing and preparation method and application thereof |
| CN114767923B (en) * | 2022-03-24 | 2023-07-11 | 金发科技股份有限公司 | Composite hydrogel silver-loaded long-acting antibacterial dressing and preparation method and application thereof |
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Application publication date: 20170222 |