CN108751427A - Handle the preparation method of heavy metal wastewater thereby Rhizopus oryzae pompon - Google Patents

Handle the preparation method of heavy metal wastewater thereby Rhizopus oryzae pompon Download PDF

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CN108751427A
CN108751427A CN201810573043.7A CN201810573043A CN108751427A CN 108751427 A CN108751427 A CN 108751427A CN 201810573043 A CN201810573043 A CN 201810573043A CN 108751427 A CN108751427 A CN 108751427A
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rhizopus oryzae
heavy metal
pompon
starchy material
adsorption
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CN108751427B (en
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不公告发明人
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Weili Biotechnology (Weihai) Co.,Ltd.
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Changzhou Love Education Science And Technology Co Ltd
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Abstract

The invention discloses a kind of preparation methods of processing heavy metal wastewater thereby Rhizopus oryzae pompon, include the following steps, 1) it is in spherical for shape by the pseudomonas pseudoalcaligenes collected after mixing fermentation culture and saccharomyces cerevisiae mixing thalline immobilization, inside is in the immobilized cell ball of porous network structure；2) using starchy material hydrolyzate as culture medium, Rhizopus oryzae spore suspension is accessed under conditions of pH=3-6, after shaken cultivation 24-48h, is collected by filtration to obtain a diameter of 50-100um Rhizopus oryzaes pompon；3) immobilized cell ball and Rhizopus oryzae pompon are filled in biological adsorption device, which is concatenated by more adsorption columns；4) after the initial concentration of heavy metal ion≤800mg/L of heavy metal wastewater thereby adjustment, PH=5-7, biological adsorption device is flowed through, the hydraulic detention time 0.5-1h in every adsorption column.Using inhomogeneous microorganism live bacteria, under the action of different adsorption mechanisms, has to different heavy metals (Cd2+, Hg2+, Cu2+, Zn2+, Ni2+, Ag2+, Pb2+) and adsorb well.

Description

Handle the preparation method of heavy metal wastewater thereby Rhizopus oryzae pompon
Technical field
The present invention relates to sewage treatment field, more particularly to a kind of preparation of processing heavy metal wastewater thereby Rhizopus oryzae pompon Method.
Background technology
At present, process for treating heavy-metal waste water mainly has three classes:Chemical reaction method, concentration and separation method and biological adsorption Method.The first kind be heavy metal ions in wastewater by chemically react the method removed include neutralization precipitation method, sulphide precipitation, Ferrite coprecipitation, chemical reduction method, electrochemical reducing and high molecular heavy metals trapping agent method etc.；Second class is to make waste water In the heavy metal method that is concentrated, detached under conditions of not changing its chemical form, including adsorbent absorption, ion hands over Change with UF membrane etc.；Third class biosorption process is a heavy metal-polluted method for treating water of new development in this year, by be micro- Biology and its derivative carry out the process of adsorbing heavy metal in water.
Compared with traditional adsorption method, biosorption process has the following advantages：1) at low concentrations, metal can be selected The removal of property；2) energy saving, treatment effeciency is high；3) it is easily isolated and recycled heavy metal 5) adsorbent is easy to regeneration.
The key of biosorption technology is to select suitable biological adsorption agent.Biological adsorption agent mostly uses live body and inactivation Two kinds of biological cell.Compared with inactivation biological cell, the adsorbance of living body biological cell absorption is more larger, adsorption process point 2 stages.1st stage is unrelated with metabolism, for biological adsorption process, carries out comparatively fast, in the process, metal ion can be by matching One or more of the effects that position, chelating are with ion exchange, physical absorption and microdeposit is compound to cell surface；2nd stage For biological cumulative process, progress is slower, and in the process, metal is transported into the cell.
But at present in document more will lose activity using single kind microorganism as biological adsorption agent, or mostly Microorganism and common adsorbent, such as activated carbon, humic acid, sepiolite, polysaccharide resins are mixed with.Biological adsorption Mechanism is often different and different because of strain, and the source of biomaterial is very extensive, thus selects suitable strain as absorption Agent is particularly important.
Invention content
The present invention provides a kind of preparation method for handling the biological adsorption agent of heavy metal wastewater thereby, using inhomogeneous micro- Biologic live bacteria, under the action of different adsorption mechanisms, to different heavy metal (Cd2+, Hg2+, Cu2+, Zn2+, Ni2+, Ag2 +, Pb2+) it is adsorbed.
To solve the above-mentioned problems, the technical solution adopted by the present invention is such：A kind of side of processing heavy metal wastewater thereby Method includes the following steps,
1) preparation of immobilized cell ball：A) by polyvinyl alcohol, calcium alginate, acryloyl and water in mass ratio 6~15：0.5~ 1.5：3~5：After 100 ratios are mixed, the polyvinyl alcohol hydrosol is obtained, the class collected after mixing fermentation culture production alkali is false single Born of the same parents bacterium and saccharomyces cerevisiae thalline are added forms mixed solution into the polyvinyl alcohol hydrosol, wherein Pseudomonas alcaligenes and wine brewing The additive amount of yeast thalline meets the Pseudomonas alcaligenes of addition 4-6g weight in wet bases and wine brewing in the polyvinyl alcohol hydrosol per 100mL Yeast Mixed Microbes；B) mixed solution is added to peristaltic pump containing 3~5w/v%N '-methylene-bisacrylamides and 1-2w/v% In the saturation boric acid solution of CaSO4, gel ball is formed, cooling 5-10h, thaws, then set again at room temperature under the conditions of placing -20 DEG C Gel ball input mass concentration after thawing at room temperature, is in 0.2~0.5g/L phosphate by the cooling 1-2h under the conditions of -10 DEG C, Shape is obtained in spherical, the internal immobilized cell ball in porous network structure；
2) preparation of Rhizopus oryzae pompon：A) commercially available starchy material is crushed, it is 500-1000 microns to obtain average grain diameter Micro- starchy material；B after micro- starchy material and water) are mixed into paste with the ratio of 150-200%, it is heated to 40-70 DEG C, Be added amylase and plant rennet, amylase enzyme concentration not Wei the micro- starchy materials of 1200-1500U/g, plant rennet Dosage is the 0.1-0.2wt% of micro- starchy material, then passes through the silk screen filter of 800-1000 mesh while hot, obtains uniform filter Liquid adds water sugar addition after filtrate cooling, and it is 20-30g/L starchy material hydrolyzates, gained starchy material water that total reducing sugar, which is made, By being formed with starchiness hydrolytic residue particle with starchiness hydrolysis clear liquid, the starchiness hydrolysis clear liquid hydrolyzes solution liquid with starchiness The weight ratio of particle is 90-95：5-10；C) using starchy material hydrolyzate as culture medium, rice is accessed under conditions of pH=3-6 Head mold spore suspension, spore a concentration of 105-106/mL in the medium after inoculation, in 25-30 DEG C, 200-250rpm's Under rotating speed, after shaken cultivation 24-48h, it is collected by filtration to obtain a diameter of 50-100um Rhizopus oryzaes pompon；
3) preparation of biological adsorption device：The biological adsorption device is concatenated by more adsorption columns, the adsorption column lower part It is provided with water inlet on side wall, overfall is provided in adsorption column upper portion side wall, upper screen cloth and lower screen cloth are provided in adsorption column, Upper screen cloth position is higher than the position where water inlet, and lower screen cloth position is less than the position where overfall, described Immobilized cell ball and Rhizopus oryzae pompon are filled in the adsorption space that adsorption column side wall is surrounded with upper and lower sieve, it is fixed The volume packing ratio for changing cell ball and Rhizopus oryzae pompon is 60-90:10-40, the two are 50- in the total filling rate of adsorption space 80%, also set up in the adsorption space equipped with annular guide shell, the top and bottom of the annular guide shell respectively with upper sieve Net and lower screen cloth do not contact, and absorption column bottom is provided with aerator, which is located at below annular guide shell；
4) processing of heavy metal wastewater thereby：Initial concentration of heavy metal ion adjustment≤800mg/L, PH in heavy metal wastewater thereby are adjusted to After 5-7, followed by biological adsorption device, the hydraulic detention time 0.5-1h in every adsorption column.
Preferably, the mixing fermentation culture process of pseudomonas pseudoalcaligenes and saccharomyces cerevisiae is：
A) the culture of saccharomyces cerevisiae seed liquor
Saccharomyces cerevisiae strain on inclined-plane is inoculated in primary-seed medium and carries out shaking flask culture, at 27-30 DEG C, rotating speed 260rmp After culture for 24 hours, it is inoculated in secondary seed medium and carries out fermentation tank culture, at 30-32 DEG C, rotating speed 260-280rmp, ventilatory capacity The seed liquor that saccharomyces cerevisiae is obtained after 2.5~3m3/h cultures 24-36h, wherein the primary-seed medium is：Yeast extract 5g/ L, peptone 6g/L, glucose 20g/L；Secondary seed medium is：Glucose 40-50g/L, yeast extract 5-8g/L, albumen Peptone 5-8g/L, (NH4) 2SO42-3g/L, MgSO47H2O0.25-0.3g/L, NaCl0.15-0.2g/L, KH2PO42.8-3g/ L；
B) the culture of Pseudomonas alcaligenes seed liquor
Pseudomonas alcaligenes strain on inclined-plane is inoculated in primary-seed medium and carries out shaking flask culture, at 27-30 DEG C, rotating speed After 160-180rmp cultures for 24 hours, it is inoculated in secondary seed medium and carries out fermentation tank culture, at 27-30 DEG C, rotating speed 160- 180rmp, 2.5~3m3/h of ventilatory capacity cultivate 24-36h, obtain the seed liquor of Pseudomonas alcaligenes, wherein the first order seed Culture medium is：Glucose 20-30g/L, yeast extract 5-8g/L, peptone 6-8g/L；The secondary seed medium is：Grape Sugared 20-30g/L, beancake powder 10-12g/L, KNO31-1.2g/L, KH2PO41-1.2g/L, FeCl26H2O0.05-0.08g/ L, CaCl27H2O0.02-0.04g/L, MgSO47H2O1-1.2g/L；
C) mixed fermentation
By the 1-3 by volume of the Pseudomonas alcaligenes seed liquor obtained by the saccharomyces cerevisiae seed liquor and step 2) obtained by step 1): 1-3 is mixed, and is transferred in fermentation medium with the inoculum concentration of 10-20% and is carried out High Density Cultivation, at 30 DEG C, rotating speed 220- 260rmp, 5~10m3/h of ventilatory capacity cultivate 36-48h, and the culture solution after fermenting is stood, the production of centrifugal enrichment thalline, as class Pseudomonas alcaligenes and saccharomyces cerevisiae thalline, wherein the fermentation medium is：Glucose sugar 80-100g/L, yeast extract 5-10g/ L, peptone 5-10g/L, beancake powder 10-12g/L, KNO31-1.2g/L, KH2PO42-2.5g/L, FeCl26H2O0.05- 0.08g/L, CaCl27H2O0.02-0.04g/L, MgSO47H2O1.5-1.8g/L, NaCl0.15-0.18g/L；
Preferably, the starchy material is corn flour, de- embryo corn flour or tapioca starch.
It is further preferred that the starchy material is corn flour.
Present invention employs common fixation support-polyvinyl alcohol, and Pseudomonas alcaligenes and saccharomyces cerevisiae are fixed, It has that intensity is high, chemical stability is good, Resistance to microbes performance is strong, nontoxic to microorganism, cheap etc. a series of excellent Point, the middle agglomeration problem for introducing calcium alginate and solving particle, enhances the balling-up ability of polyvinyl alcohol gel on this basis, In addition, the addition of calcium alginate and CaSO4 strengthen gel particle intensity, while also introducing acrylamide polymerization reaction, it is allowed to Inside polyvinyl alcohol gel particle and surface forms polyacrylamide reticular structure, effectively improves polyvinyl alcohol gel Water-soluble dilatancy makes polyvinyl alcohol immobilized microorganism have comparatively ideal physics (particle size) and mechanical performance, significantly Pseudomonas alcaligenes is increased with saccharomyces cerevisiae to the tolerance of toxic metal ions, has achieved the effect that recycle.
Because of Rhizopus oryzae because itself has the characteristics that balling-up, it is not necessarily to immobilization, the present invention directly has chosen starchy material Hydrolyzate cultivates it, and the starchy material hydrolyzate is by 5-15wt% starchiness hydrolytic residue particles and 70- 80wt% starchiness hydrolysis clear liquid composition, wherein starchiness hydrolysis clear liquid can be provided for the production of Rhizopus oryzae carbon source, nitrogen source and Other trace elements, and starchiness hydrolytic residue particle provides carrier for the growth of spore, is molded with control Rhizopus oryzae is commonly used to Calcium carbonate etc. compare, have innate advantage, uniformly, being dispersed in starchiness liquefaction clear liquid of stablizing, so that rice root Mould spore is constantly wrapped in after developing for mycelium in starchy material hydrolyzate in residue particles, and then formation densification, And have the mycelium pellet of certain mechanical strength, through lot of experiment validation, using starchy material hydrolyzate, without adding any object In the case of matter, the balling-up well of Rhizopus oryzae spore liquid energy, and spherical size uniform (ball 50-100um), have it is good Mechanical performance, biomass are big, and chitin content is high, big to heavy metal ion adsorbed ability in fillable adsorption column.
With prior art ratio, the present invention has chosen the viable bacteria of Pseudomonas alcaligenes, saccharomyces cerevisiae and Rhizopus oryzae as life Object adsorbent, wherein saccharomyces cerevisiae be because it is the generally acknowledged fungi that most metal ions are all had with stronger adsorption capacity, Rhizopus oryzae because in its cell wall chitin and chitosan content it is higher, functional group-COOH of cell surface ,-NH2 ,- OH can and metal ion combination, to by cell surface adsorb or be complexed reach to adsorption of metal ions purpose；It is false to produce alkali For zygosaccharomyces in bacterium class, adsorption mechanism is mainly intracellular absorption, is the complexing of cell surface first, followed by into bacterium The slow diffusion process in portion, these three bacterium whiles, are applied, can be under the action of different adsorption mechanisms, to different heavy metals (Cd2+, Hg2+, Cu2+, Zn2+, Ni2+, Ag2+, Pb2+) all has suction-operated well.As immobilized cell ball and meter Gen Mould pompon is filled in when being adsorbed in bioreactor, due to the effect of aerator, immobilized cell ball and Rhizopus oryzae Mycelium pellet is suspended in the adsorption space that upper screen cloth, lower screen cloth and adsorption column side wall are surrounded, and in annular stream guiding barrel shape At circulation, enhance the contact of immobilized cell ball and Rhizopus oryzae pompon with waste water, so both strengthen to heavy metal from Son absorption.
Description of the drawings
Fig. 1 is the structural schematic diagram of biological absorber.
Specific implementation mode
In order to deepen the understanding of the present invention, below in conjunction with embodiment, the invention will be further described, these implementations Example is only used for explaining the present invention, is not intended to limit the scope of the present invention..
In following embodiment, the method for Yield of chitin is：By mycelium, drying to constant weight, takes 40g, and 0.5mol/L is added Hydrochloric acid solution, boiling water bath 1h, is washed to neutrality, and solid is collected by centrifugation；15% sodium hydroxide solution is added, boiling water bath 30min takes off Go out protein, be washed till neutrality, solid is collected by centrifugation, adds appropriate oxalic acid solution in 70 DEG C of warm bath 30min, be washed to neutrality, Solid, as chitin is collected by centrifugation；50% sodium hydroxide solution, 121 DEG C of processing 2.5h deacetylations are added, are washed to neutrality, It is collected by centrifugation solid, is added 0.1mol/L hydrochloric acid solutions, boiling water bath 2h, then with sodium hydroxide solution tune pH10, it is heavy to be collected by centrifugation It forms sediment, chitosan is obtained after dry.
The mixing fermentation culture process of 1 pseudomonas pseudoalcaligenes of embodiment and saccharomyces cerevisiae, includes the following steps：
1) culture of saccharomyces cerevisiae seed liquor
Saccharomyces cerevisiae slant strains are inoculated in primary-seed medium and carry out shaking flask culture, at 27-30 DEG C, rotating speed 260rmp is trained After supporting for 24 hours, it is inoculated in secondary seed medium and carries out fermentation tank culture, at 30 DEG C, rotating speed 260rmp, 2.5~3m3/h of ventilatory capacity Culture obtains the seed liquor of saccharomyces cerevisiae afterwards for 24 hours, wherein the primary-seed medium is：Yeast extract 5g/L, peptone 6g/L, Glucose 20g/L；Secondary seed medium is：Glucose 40g/L, yeast extract 5g/L, peptone 5g/L, (NH4) 2SO42g/ L, MgSO47H2O0.25g/L, NaCl0.15g/L, KH2PO42.8g/L.
2) culture of Pseudomonas alcaligenes seed liquor
Monad slant strains are inoculated in primary-seed medium and carry out shaking flask culture, and at 27-30 DEG C, rotating speed 160rmp is cultivated It after for 24 hours, is inoculated in secondary seed medium and carries out fermentation tank culture, at 30 DEG C, rotating speed 160rmp, 2.5~3m3/h of ventilatory capacity is trained It supports for 24 hours, obtains the seed liquor of saccharomyces cerevisiae, wherein the primary-seed medium is：Glucose 20g/L, yeast extract 5g/L, Peptone 6g/L；The secondary seed medium is：Glucose 20g/L, beancake powder 10g/L, KNO31g/L, KH2PO41g/ L, FeCl26H2O0.05g/L, CaCl27H2O0.02g/L, MgSO47H2O1g/L；
3) mixed fermentation
By the 1-3 by volume of the Pseudomonas alcaligenes seed liquor obtained by the saccharomyces cerevisiae seed liquor and step 2) obtained by step 1): 1-3 is mixed, and is transferred in fermentation medium with 20% inoculum concentration and is carried out High Density Cultivation, at 30 DEG C, rotating speed 260rmp, is led to 5~10m3/h of tolerance cultivates 36-48h, and the culture solution after fermenting is stood, centrifugal enrichment thalline, the fermentation medium For：Glucose sugar 80g/L, yeast extract 5g/L, peptone 5g/L, beancake powder 10g/L, KNO31g/L, KH2PO42g/L, FeCl2 6H2O0.05g/L CaCl27H2O0.02g/L, MgSO47H2O1.5g/L, NaCl0.15g/L.
A kind of preparation method of 2 immobilized cell ball of embodiment, includes the following steps：
A) by polyvinyl alcohol, calcium alginate, acryloyl and water in mass ratio 12：1：4：After 100 ratios are mixed, polyethylene is obtained The alcohol hydrosol pseudomonas pseudoalcaligenes collected after mixing fermentation culture and saccharomyces cerevisiae thalline is added water-soluble to polyvinyl alcohol Mixed solution is formed in glue, wherein the additive amount of Pseudomonas alcaligenes and saccharomyces cerevisiae thalline meets the polyvinyl alcohol per 100mL The Pseudomonas alcaligenes and saccharomyces cerevisiae Mixed Microbes of 5g weight in wet bases are added in the hydrosol；B mixed solution is added to peristaltic pump) and is contained In the saturation boric acid solution of 4w/v%N '-methylene-bisacrylamides and 1.5w/v%CaSO4, gel ball is formed, places -20 DEG C Under the conditions of cooling 5-10h, thaw at room temperature, cooling 1-2h under the conditions of being then placed in -10 DEG C again, after thawing at room temperature, by gel It is to obtain shape in spherical, the internal immobilized cell in porous network structure in 0.3g/L phosphate that ball, which puts into mass concentration, Ball A.
A kind of preparation method of 3 immobilized cell ball of embodiment, includes the following steps：
A) by polyvinyl alcohol, calcium alginate, acryloyl and water in mass ratio 6：0.5：3：After 100 ratios are mixed, poly- second is obtained The pseudomonas pseudoalcaligenes collected after mixing fermentation culture and saccharomyces cerevisiae thalline are added to polyvinyl alcohol water the enol hydrosol Mixed solution is formed in colloidal sol, and controls poly- second of the additive amount satisfaction of Pseudomonas alcaligenes and saccharomyces cerevisiae thalline per 100mL The Pseudomonas alcaligenes and saccharomyces cerevisiae Mixed Microbes of 4g weight in wet bases are added in the enol hydrosol；Mixed solution is added with peristaltic pump again Into the saturation boric acid solution containing 3w/v%N '-methylene-bisacrylamides and 1w/v%CaSO4, gel ball is formed, places -20 Cooling 5-10h, thaws at room temperature under the conditions of DEG C, and cooling 1-2h will coagulate after thawing at room temperature under the conditions of being then placed in -10 DEG C again It is to obtain shape in spherical, the internal fixation cell in porous network structure in 0.2g/L phosphate that glueballs, which puts into mass concentration, Born of the same parents' ball B.
A kind of preparation method of 4 immobilized cell ball of embodiment, includes the following steps：
A) by polyvinyl alcohol, calcium alginate, acryloyl and water in mass ratio 15：1.5：5：After 100 ratios are mixed, poly- second is obtained The pseudomonas pseudoalcaligenes collected after mixing fermentation culture and saccharomyces cerevisiae thalline are added to polyvinyl alcohol water the enol hydrosol Mixed solution is formed in colloidal sol, wherein the additive amount of Pseudomonas alcaligenes and saccharomyces cerevisiae thalline meets the polyethylene per 100mL The Pseudomonas alcaligenes and saccharomyces cerevisiae Mixed Microbes of 6g weight in wet bases are added in the alcohol hydrosol；B) mixed solution is added to peristaltic pump In saturation boric acid solution containing 5w/v%N '-methylene-bisacrylamides and 2w/v%CaSO4, gel ball is formed, places -20 DEG C Under the conditions of cooling 5-10h, thaw at room temperature, cooling 1-2h under the conditions of being then placed in -10 DEG C again, after thawing at room temperature, by gel It is to obtain shape in spherical, the internal immobilized cell in porous network structure in 0.5g/L phosphate that ball, which puts into mass concentration, Ball C.
A kind of preparation method of 5 Rhizopus oryzae pompon of embodiment, includes the following steps：
A) commercially available corn flour is crushed, obtains micro- corn flour that average grain diameter is 800 microns；B) by micro- corn flour and water with After 180% ratio is mixed into paste, it is heated to 40-70 DEG C, amylase is added and plant rennet, amylase enzyme concentration are The micro- corn flour of 1300U/g, the dosage of plant rennet are the 0.15wt% of micro- corn flour, then pass through 800-1000 purposes while hot Silk screen filter obtains uniform filtrate, adds water sugar addition after filtrate cooling, and it is 30g/L corn flour hydrolyzates, gained that total reducing sugar, which is made, Corn flour hydrolyzate is made of corn flour hydrolysis clear liquid with corn flour hydrolytic residue particle, the corn flour hydrolysis clear liquid and corn The weight ratio that powder hydrolyzes particle is 90-95:5-10；C) it using corn flour hydrolyzate as culture medium, is accessed under conditions of pH=3-6 Rhizopus oryzae spore suspension, spore a concentration of 105-106/mL in the medium after inoculation, in 25-30 DEG C, 200-250rpm Rotating speed under, after shaken cultivation 24-48h, be collected by filtration to obtain 50-100um Rhizopus oryzaes pompon A, dry cell weight 5.7g/L, Extracted and measurement, chitin content reach 25.6% (g/g dry myceliums).
A kind of preparation method of 6 Rhizopus oryzae pompon of embodiment, includes the following steps：
1) preparation of embryo corn flour hydrolyzate is taken off：A) commercially available de- embryo corn flour is crushed, it is 500 microns micro- to obtain average grain diameter De- embryo corn flour；B after micro- de- embryo corn flour and water) are mixed into paste with 150% ratio, it is heated to 40-70 DEG C, is added and forms sediment Powder enzyme and plant rennet, amylase enzyme concentration are the micro- de- embryo corn flour of 1200U/g, and the dosage of plant rennet is that micro- de- embryo is beautiful Then the 0.1wt% of rice flour passes through the silk screen filter of 800-1000 mesh while hot, obtain uniform filtrate, add water tune after filtrate cooling Whole pol, it is 30g/L corn flour hydrolyzates that total reducing sugar, which is made, gained take off embryo corn flour hydrolyzate by de- embryo corn flour hydrolyze clear liquid with De- embryo corn flour hydrolytic residue particle composition, the weight ratio of the de- embryo corn flour hydrolysis clear liquid and de- embryo corn flour hydrolysis particle For 90-95：5-10；C) using de- embryo corn flour hydrolyzate as culture medium, Rhizopus oryzae spore suspension is accessed under conditions of pH=3-6 Liquid, a concentration of 105-106/mL vibrates training to spore under 25-30 DEG C, the rotating speed of 200-250rpm in the medium after inoculation After supporting 24-48h, it is collected by filtration to obtain 50-100um Rhizopus oryzae pompon B, dry cell weight 5.3g/L is extracted and measure, several Fourth matter content reaches 22.6% (g/g dry myceliums).
A kind of preparation method of 7 Rhizopus oryzae pompon of embodiment, includes the following steps：
1) preparation of tapioca starch hydrolyzate：A) commercially available tapioca starch is crushed, obtains micro- tapioca starch that average grain diameter is 1000；B) will After tapioca starch and water are mixed into paste with 200% ratio, it is heated to 40-70 DEG C, amylase and plant rennet, starch is added Enzyme enzyme concentration is the micro- tapioca starch of 1500U/g, and the dosage of plant rennet is the 0.2wt% of micro- tapioca starch, is then passed through while hot The silk screen filter of 800-1000 mesh obtains uniform filtrate, adds water sugar addition after filtrate cooling, and it is 30g/L cassavas that total reducing sugar, which is made, Powder hydrolyzate, gained tapioca starch hydrolyzate are made of tapioca starch hydrolysis clear liquid with corn flour hydrolytic residue particle, the tapioca starch The weight ratio for hydrolyzing clear liquid and tapioca starch hydrolysis particle is 90-95：5-10；C) using tapioca starch hydrolyzate as culture medium, in pH= Rhizopus oryzae spore suspension is accessed under conditions of 3-6, spore a concentration of 105-106/mL in the medium after inoculation, in 25- It 30 DEG C, under the rotating speed of 200-250rpm, after shaken cultivation 24-48h, is collected by filtration to obtain 50-100um Rhizopus oryzae pompon C, bacterium Soma weight is 5.35g/L, and extracted and measurement, chitin content reach 23.2% (g/g dry myceliums).
The Rhizopus oryzae pompon of Application Example 2,3 or 4 gained immobilized cell balls and 5,6 or 7 gained of embodiment carries out The processing method of heavy metal wastewater thereby, includes the following steps：
1) preparation of biological adsorption device：As shown in Figure 1, the biological adsorption device is concatenated by 2 adsorption columns 1, it is described It is provided with water inlet on adsorption column lower sides, overfall is provided in adsorption column upper portion side wall, sieve is provided in adsorption column 1 Net 2 and lower screen cloth 3,2 position of upper screen cloth are higher than the position where water inlet, and 3 position of lower screen cloth is less than overfall institute Position, be filled with immobilized cell ball and rice in the adsorption space that the adsorption column side wall and upper and lower sieve are surrounded The volume packing ratio of head mold mycelium pellet, immobilized cell ball and Rhizopus oryzae pompon is 60-90:10-40, the two is in adsorption space Total filling rate is 60%, is also set up in the adsorption space equipped with annular guide shell 4, the upper end of the annular guide shell 4 is under End is not contacted with upper screen cloth and lower screen cloth respectively, and absorption column bottom is provided with aerator 5, which leads positioned at annular 4 lower section of flow cartridge.
2) processing of heavy metal wastewater thereby：By initial concentration of heavy metal ion adjustment≤800mg/L, PH tune in heavy metal wastewater thereby It is whole for after 5-7, followed by biological adsorption device, the hydraulic detention time 0.5-1h in every adsorption column.
Immobilized cell ball A and Rhizopus oryzae pompon A, immobilized cell ball B and Rhizopus oryzae pompon B, immobilized cell Ball C is filled in above-mentioned different bioreactor respectively from Rhizopus oryzae pompon C, immobilized cell ball A and Rhizopus oryzae pompon C Adsorption column in, be respectively designated as the first biological adsorption device, the second biological adsorption device, third biological adsorption device, the 4th biology inhale Adnexa, wherein the packing ratio of immobilized cell ball A and Rhizopus oryzae pompon A is 80 in the first biological adsorption device:20, The packing ratio of immobilized cell ball B and Rhizopus oryzae pompon B is 60 in two biological adsorption devices:40, in third biological adsorption device The packing ratio of immobilized cell ball C and Rhizopus oryzae pompon C is 90:10, immobilized cell ball A are filled out with Rhizopus oryzae pompon C's It fills than being 70:30, prepare the mixed solution containing 30mg/LCu2+, Zn2+, Ni2+, Cd2+, Cr2+, Hg2+, Pb2+ respectively, adjustment After pH=5-7, the concentration variation such as table 1 of each biological adsorption device are passed in and out：
The above embodiments merely illustrate the technical concept and features of the present invention, and its object is to allow person skilled in the art's energy Solution present disclosure much of that is simultaneously implemented according to this, and it is not intended to limit the scope of the present invention, all according to spirit of that invention Equivalent change or modification made by essence, should be covered by the protection scope of the present invention.

Claims (2)

1. a kind of preparation method of Rhizopus oryzae pompon, which is characterized in that including：A) commercially available starchy material is crushed, is obtained Micro- starchy material that average grain diameter is 500-1000 microns；B) micro- starchy material and water are mixed with the ratio of 150-200% After paste, it is heated to 40-70 DEG C, amylase and plant rennet is added, amylase enzyme concentration is the micro- shallow lakes 1200-1500U/g Silty raw material, the dosage of plant rennet are the 0.1-0.2wt% of micro- starchy material, then pass through 800-1000 purposes while hot Silk screen filter obtains uniform filtrate, adds water sugar addition after filtrate cooling, total reducing sugar is made and is hydrolyzed for 20-30g/L starchy materials Liquid, gained starchy material hydrolyzate are made of starchiness hydrolysis clear liquid with starchiness hydrolytic residue particle, the starchiness water The weight ratio for solving clear liquid and starchiness hydrolysis particle is 90-95：5-10；C) using starchy material hydrolyzate as culture medium, pH= Rhizopus oryzae spore suspension is accessed under conditions of 3-6, spore a concentration of 105-106/mL in the medium after inoculation, in 25- It 30 DEG C, under the rotating speed of 200-250rpm, after shaken cultivation 24-48h, is collected by filtration to obtain a diameter of 50-100um Rhizopus oryzaes mycelia Ball.
2. a kind of method of processing heavy metal wastewater thereby, which is characterized in that use Rhizopus oryzae pompon described in claim 1.
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