CN102351320A - Method for preparing novel biological microcapsule for biological fluidized bed - Google Patents
Method for preparing novel biological microcapsule for biological fluidized bed Download PDFInfo
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- CN102351320A CN102351320A CN2011101833042A CN201110183304A CN102351320A CN 102351320 A CN102351320 A CN 102351320A CN 2011101833042 A CN2011101833042 A CN 2011101833042A CN 201110183304 A CN201110183304 A CN 201110183304A CN 102351320 A CN102351320 A CN 102351320A
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- microcapsule
- sodium alginate
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- fluidized bed
- chitosan
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- 239000003094 microcapsule Substances 0.000 title claims abstract description 83
- 238000000034 method Methods 0.000 title abstract description 14
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims abstract description 31
- 239000000661 sodium alginate Substances 0.000 claims abstract description 31
- 235000010413 sodium alginate Nutrition 0.000 claims abstract description 31
- 229940005550 sodium alginate Drugs 0.000 claims abstract description 31
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 29
- 229920001661 Chitosan Polymers 0.000 claims abstract description 22
- 241000894006 Bacteria Species 0.000 claims abstract description 19
- 238000006731 degradation reaction Methods 0.000 claims abstract description 10
- 230000015556 catabolic process Effects 0.000 claims abstract description 9
- 239000011324 bead Substances 0.000 claims abstract description 8
- 239000000126 substance Substances 0.000 claims abstract description 7
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims abstract description 6
- 150000001768 cations Chemical class 0.000 claims abstract description 6
- 239000000648 calcium alginate Substances 0.000 claims abstract description 5
- 235000010410 calcium alginate Nutrition 0.000 claims abstract description 5
- 229960002681 calcium alginate Drugs 0.000 claims abstract description 5
- OKHHGHGGPDJQHR-YMOPUZKJSA-L calcium;(2s,3s,4s,5s,6r)-6-[(2r,3s,4r,5s,6r)-2-carboxy-6-[(2r,3s,4r,5s,6r)-2-carboxylato-4,5,6-trihydroxyoxan-3-yl]oxy-4,5-dihydroxyoxan-3-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylate Chemical compound [Ca+2].O[C@@H]1[C@H](O)[C@H](O)O[C@@H](C([O-])=O)[C@H]1O[C@H]1[C@@H](O)[C@@H](O)[C@H](O[C@H]2[C@H]([C@@H](O)[C@H](O)[C@H](O2)C([O-])=O)O)[C@H](C(O)=O)O1 OKHHGHGGPDJQHR-YMOPUZKJSA-L 0.000 claims abstract description 5
- 239000001509 sodium citrate Substances 0.000 claims abstract description 5
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims abstract description 5
- 239000012528 membrane Substances 0.000 claims abstract description 4
- 229920002535 Polyethylene Glycol 1500 Polymers 0.000 claims abstract description 3
- 229920001030 Polyethylene Glycol 4000 Polymers 0.000 claims abstract description 3
- 239000000243 solution Substances 0.000 claims description 28
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 20
- 238000002360 preparation method Methods 0.000 claims description 12
- 239000008223 sterile water Substances 0.000 claims description 12
- 244000005700 microbiome Species 0.000 claims description 9
- FHVDTGUDJYJELY-UHFFFAOYSA-N 6-{[2-carboxy-4,5-dihydroxy-6-(phosphanyloxy)oxan-3-yl]oxy}-4,5-dihydroxy-3-phosphanyloxane-2-carboxylic acid Chemical compound O1C(C(O)=O)C(P)C(O)C(O)C1OC1C(C(O)=O)OC(OP)C(O)C1O FHVDTGUDJYJELY-UHFFFAOYSA-N 0.000 claims description 8
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 8
- 229940072056 alginate Drugs 0.000 claims description 8
- 235000010443 alginic acid Nutrition 0.000 claims description 8
- 229920000615 alginic acid Polymers 0.000 claims description 8
- 239000011575 calcium Substances 0.000 claims description 8
- 229910052791 calcium Inorganic materials 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 8
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 8
- 229920000053 polysorbate 80 Polymers 0.000 claims description 8
- 239000002351 wastewater Substances 0.000 claims description 7
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 claims description 4
- 229910001424 calcium ion Inorganic materials 0.000 claims description 4
- 210000002808 connective tissue Anatomy 0.000 claims description 4
- 239000003995 emulsifying agent Substances 0.000 claims description 4
- 239000007924 injection Substances 0.000 claims description 4
- 238000002347 injection Methods 0.000 claims description 4
- 239000011259 mixed solution Substances 0.000 claims description 4
- 230000002572 peristaltic effect Effects 0.000 claims description 4
- 239000002504 physiological saline solution Substances 0.000 claims description 4
- 229920003023 plastic Polymers 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- 150000003384 small molecules Chemical class 0.000 claims description 3
- 239000002253 acid Substances 0.000 claims description 2
- MTHSVFCYNBDYFN-UHFFFAOYSA-N diethylene glycol Chemical compound OCCOCCO MTHSVFCYNBDYFN-UHFFFAOYSA-N 0.000 claims description 2
- 150000002500 ions Chemical class 0.000 claims description 2
- 206010011732 Cyst Diseases 0.000 claims 1
- 208000031513 cyst Diseases 0.000 claims 1
- 239000002775 capsule Substances 0.000 abstract description 6
- 239000000463 material Substances 0.000 abstract description 3
- 239000001110 calcium chloride Substances 0.000 abstract 2
- 229910001628 calcium chloride Inorganic materials 0.000 abstract 2
- 239000011248 coating agent Substances 0.000 abstract 2
- 238000000576 coating method Methods 0.000 abstract 2
- 230000002378 acidificating effect Effects 0.000 abstract 1
- 150000001450 anions Chemical class 0.000 abstract 1
- 229910052799 carbon Inorganic materials 0.000 abstract 1
- 238000005342 ion exchange Methods 0.000 abstract 1
- VUYXVWGKCKTUMF-UHFFFAOYSA-N tetratriacontaethylene glycol monomethyl ether Chemical compound COCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCO VUYXVWGKCKTUMF-UHFFFAOYSA-N 0.000 abstract 1
- RFFLAFLAYFXFSW-UHFFFAOYSA-N 1,2-dichlorobenzene Chemical compound ClC1=CC=CC=C1Cl RFFLAFLAYFXFSW-UHFFFAOYSA-N 0.000 description 18
- 230000000593 degrading effect Effects 0.000 description 8
- 230000000694 effects Effects 0.000 description 7
- 238000005516 engineering process Methods 0.000 description 7
- 239000000499 gel Substances 0.000 description 5
- WXNZTHHGJRFXKQ-UHFFFAOYSA-N 4-chlorophenol Chemical compound OC1=CC=C(Cl)C=C1 WXNZTHHGJRFXKQ-UHFFFAOYSA-N 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 229940090668 parachlorophenol Drugs 0.000 description 3
- 238000004065 wastewater treatment Methods 0.000 description 3
- 238000005273 aeration Methods 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 238000004817 gas chromatography Methods 0.000 description 2
- 239000011824 nuclear material Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229920000867 polyelectrolyte Polymers 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 239000004372 Polyvinyl alcohol Substances 0.000 description 1
- 229920001131 Pulp (paper) Polymers 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 239000005515 coenzyme Substances 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 229920005615 natural polymer Polymers 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920002451 polyvinyl alcohol Polymers 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
Landscapes
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a method for preparing a novel biological microcapsule for a biological fluidized bed. The microcapsule is prepared through treating sodium alginate, powdery active carbon, calcium chloride and chitosan as capsule materials, and a dominant degradation bacterium as a capsule core, preparing calcium alginate gel beads through carrying out ion exchange on sodium alginate and calcium chloride, coating the surface of the gel beads with chitosan, liquefying the gel beads in sodium citrate, and coating the gel beads with sodium alginate. The diameter of the prepared microcapsule is 3.0-4.0mm, the membrane thickness of the prepared microcapsule is 10-20mum, the density of the prepared microcapsule is 1.05-1.08g/ml, the maximum mechanical strength of the prepared microcapsule is 730.31g/cm<2>, and the maximum interception molecular weight of the prepared microcapsule is about PEG 4000, so a micromolecular substance with the relative molecular weight of less than PEG 1500 can freely go through the capsule membrane. Acidic conditions and most divalent cations allow the microcapsule to be stable, and monovalent cations and most anions are bad for the stability of the microcapsule.
Description
One, technical field
The invention belongs to the microbiological treatment technical field of sewage, be specifically related to a kind of preparation method who is applied to the novel biology microcapsule of biological fluidized bed wastewater treatment.
Two, background technology
The microencapsulation technology is that enzyme, coenzyme, protein and other or microorganism, animal and plant cells are encapsulated in formed pearl in the hydrophilic semi-permeable membranes of one deck, ellipticity or ellipsoid shape microcapsule; Make biomacromolecule and cell be blocked in the film or film outside; And allow required oxygen, nutritive substance and other small-molecule substances of cell freely to get into and freely the discharging of capsule intracellular metabolite product, thereby reach catalysis, cultivation or immune isolated purpose.At present, the bio-microcapsule technology all has application in every field such as food and fermentation industries, chemosynthesis industry, environmental pollution improvements.
The method of microencapsulation technology imbedded microbe mainly contains: absorption method, entrapping method and crosslinking, wherein the most frequently used is entrapping method.Entrapping method is that microorganism is embedded in semipermeable polymkeric substance or the film, makes microorganism get into porous carrier inside.Be characterized in that operation is simple and feasible, little to the microbic activity influence.Embedded material commonly used at present has: agar, sodium alginate, chitosan, polyvinyl alcohol, polyacrylamide etc.Wherein two kinds of natural polymers of sodium alginate and chitosan have excellent biological compatibility, and can under mild conditions, carry out polyelectrolyte complex reaction formation microcapsule, are widely used.Publication number is a kind of microcapsule that are embedded with immobilized dominant bacteria for the CN101348781A patent discloses, and are applied to the processing to wood pulp papermaking waste water, obtain effect preferably.My unit publication number is a kind of inner loop fluidized bed reactor reactor that uses the microcapsule carrier for the CN201250141Y patent discloses, and makes the microcapsule carrier be applied to biological fluidized bed and carries out wastewater treatment and become possibility.But existing entrapping method technology is poor because of its mass-transfer performance, and stability is high and influence the application of microcapsule carrier.
Three, summary of the invention
For overcoming the deficiency of prior art, the invention discloses a kind of preparation method who is applied to the embedding advantage degradation bacteria bio-microcapsule of biological fluidized bed.
The objective of the invention is to: provide technology method simple, with low cost, prepare good biocompatibility, mass-transfer performance is good, physical strength is high, the bio-microcapsule of the liquid core embedding advantage degradation bacteria of good stability.
The novel biology microcapsule preparation method of above-mentioned biological fluidized bed of the present invention may further comprise the steps:
(1) preparation of microorganism mixed solution: in sodium alginate soln, add emulsifier tween 80 and Powdered Activated Carbon and stir obtaining solution A; Wherein the sodium alginate massfraction is 2.0%; Tween 80 concentration is 0.5%～1.0%, Powdered Activated Carbon 0.75% (W/V); In solution A, adding 10%～20% (V/V) advantage degradation germ liquid mixes and obtains solution B;
(2) solution B is added drop-wise to injection system through peristaltic pump in 4% the calcium chloride solution and reacts 15min～30min, make calcium alginate plastic beads;
(3) calcium alginate plastic beads that makes is filtered, and use sterile water wash; Place pH6.0,1.8% chitosan solution to react 10min～20min then and make alginate calcium/chitosan microcapsules;
(4) alginate calcium/chitosan microcapsules that makes is filtered the back sterile water wash; Place the sodium citrate solution liquefaction 5min-12min of 55mmol/L to make the microcapsule of kernel then for liquid state;
(5) microcapsule that will make filter the back and use sterile water wash, place 0.15% sodium alginate soln overlay film 10min～20min once more then, make bio-microcapsule;
(6) microcapsule are filtered and clean with pure water, then microcapsule being placed calcium ion solubility is that 0.05～0.1% physiological saline storage solution is preserved.
Compared with prior art, the invention has the advantages that in efficient degrading bacteria embedding and the microcapsule, thereby effectively improved the treatment effect of efficient degrading bacteria, reduced the loss of microorganism waste water; Secondly; Microcapsule of the present invention have the advantage of traditional sodium alginate micro gel capsule: good biocompatibility; And cheap and easy to get, a large amount of carboxyls is arranged on the molecular chain, can under mild conditions, form polyelectrolyte film through positive and negative electric charge inspiration and many range of polycationic substances; Once more; The present invention has added an amount of Powdered Activated Carbon in the capsule material; It has very big specific surface area and pore volume; Utilize the adsorption of Powdered Activated Carbon; Can make on the one hand more micromolecular matrix degradation and dissolved oxygen be adsorbed the entering microcapsule and be concentrated in activated carbon surface and all around; For the Metabolic activity of microorganism is built good microenvironment; Accelerated organic degradation process; Also help the mass-transfer performance that improves microcapsule of the present invention on the other hand, reach better degradation effect.The maximum molecular weight cut-off of the made microcapsule of the present invention is about PEG4000; Relative molecular mass had excellent mass transferring performance less than the small-molecule substance of PEG1500; Microcapsule are stable under acid and most of divalent cation condition in wastewater treatment process, and monovalent cation and most of negatively charged ion are unfavorable for the stable of microcapsule.。At last, the diameter of microcapsule is 3.0～4.0mm, and thickness is 10～20 μ m, and density is 1.05～1.08g/ml, can reduce the energy consumption of biological fluidized bed greatly and solve the blockage problem of fluidized-bed.
The preparation technology of microcapsule of the present invention is simple, and cost is low; When being applied to biological fluidized bed processing organic wastewater with difficult degradation thereby, its operation is also very convenient, directly just can move in the microcapsule adding biological fluidized bed with the embedding dominant bacteria; Do not need significant investment; Treatment effect is good, and kinetic equation loss is low, can satisfy environmental requirement.Microcapsule of the present invention can reuse in fluidized-bed, also can reclaim wherein dominant bacteria with simple method liquefaction, can not produce bad air and mud in the technological process, help the protection of ecotope.
Four, embodiment
Embodiment 1:
A kind of novel biology microcapsule carrier of biological fluidized bed; It is characterized in that said microcapsule are made up of kernel and adventitia; Its kernel is aqueous sodium alginate gel; Include Powdered Activated Carbon and immobilized dominant bacteria in the said sodium alginate gel; Kernel is coated with chitosan; Chitosan is coated with the sodium alginate film, and the embedding bacterium in the inner nuclear material is the orthodichlorobenzene efficient degrading bacteria.
The microcapsule carrier of present embodiment is to prepare with step by the following method:
(1) preparation of microorganism mixed solution: in sodium alginate soln, add emulsifier tween 80 and Powdered Activated Carbon and stir obtaining solution A, wherein the sodium alginate massfraction is 2.0%, and tween 80 concentration is 0.5%, Powdered Activated Carbon 0.75%; In A, in solution A, adding 10%～20% (V/V) orthodichlorobenzene efficient degrading bacteria bacterium liquid mixes and obtains solution B;
(2) solution B is added drop-wise to injection system through peristaltic pump in 4% the calcium chloride solution and reacts 20min, make sodium alginate microcapsule;
(3) sodium alginate microcapsule that makes is filtered, and use sterile water wash; Place 1.8% chitosan solution (the pH value is 6.0) reaction 10min then, make alginate calcium/chitosan microcapsules;
(4) alginate calcium/chitosan microcapsules that makes is filtered the back sterile water wash; Place the sodium citrate solution liquefaction 8min of 55mmol/L to make the micro-capsule of kernel then for liquid state;
(5) micro-capsule that will make filters the back and uses sterile water wash, places 0.15% sodium alginate soln overlay film 10min once more then, makes bio-microcapsule;
(6) microcapsule are filtered and clean with pure water, then microcapsule being placed calcium ion solubility is that 0.1% physiological saline storage solution is preserved.
The above-mentioned orthodichlorobenzene efficient degrading bacteria bio-microcapsule input biological fluidized bed that is embedded with that makes is handled orthodichlorobenzene waste water; The microcapsule carrier adds 15% in biological fluidized bed; Operational conditions: the pH value is 7.0, aeration rate 120L/h, hydraulic detention time 2d.Every separated 4h gets water sample one time, and with gas chromatography determination orthodichlorobenzene concentration, with the judgment processing effect, analytical data is as shown in table 1:
Orthodichlorobenzene Inlet and outlet water concentration monitor value among table 1: the embodiment 1
Embodiment 2:
A kind of novel biology microcapsule carrier of biological fluidized bed; It is characterized in that said microcapsule are made up of kernel and adventitia; Its kernel is aqueous sodium alginate gel; Include Powdered Activated Carbon and immobilized dominant bacteria in the said sodium alginate gel; Kernel is coated with chitosan; Chitosan is coated with the sodium alginate film, and the embedding bacterium in the inner nuclear material is the para-chlorophenol efficient degrading bacteria.
The microcapsule carrier of present embodiment is to prepare with step by the following method:
(1) preparation of microorganism mixed solution: in sodium alginate soln, add emulsifier tween 80 and Powdered Activated Carbon and stir obtaining solution A, wherein the sodium alginate massfraction is 2.0%, and tween 80 concentration is 0.5%, Powdered Activated Carbon 0.75%; In solution A, adding 10%～20% (V/V) para-chlorophenol efficient degrading bacteria wet thallus mixes and obtains solution B;
(2) solution B is added drop-wise to injection system through peristaltic pump in 4% the calcium chloride solution and reacts 20min, make sodium alginate microcapsule;
(3) sodium alginate microcapsule that makes is filtered, and use sterile water wash; Place 1.8% chitosan solution (the pH value is 6.0) reaction 10min then, make alginate calcium/chitosan microcapsules;
(4) alginate calcium/chitosan microcapsules that makes is filtered the back sterile water wash; Place the sodium citrate solution liquefaction 8min of 55mmol/L to make the micro-capsule of kernel then for liquid state;
(5) micro-capsule that will make filters the back and uses sterile water wash, places 0.15% sodium alginate soln overlay film 10min once more then, makes bio-microcapsule;
(6) microcapsule are filtered and clean with pure water, then microcapsule being placed calcium ion solubility is that 0.1% physiological saline storage solution is preserved.
The above-mentioned orthodichlorobenzene efficient degrading bacteria bio-microcapsule carrier input biological fluidized bed that is embedded with that makes is handled orthodichlorobenzene waste water; The microcapsule carrier adds 15% in biological fluidized bed; Operational conditions: the pH value is 7.0, aeration rate 120L/h, hydraulic detention time 2d.Every separated 4h gets water sample one time, and with gas chromatography determination orthodichlorobenzene concentration, with the judgment processing effect, analytical data is as shown in table 2:
Para-chlorophenol Inlet and outlet water concentration monitor value among table 2: the embodiment 1
Claims (4)
1. novel biology microcapsule preparation method who is applied to biological fluidized bed; It is characterized in that said microcapsule are made up of kernel and adventitia; Its kernel is aqueous sodium alginate gel; Include Powdered Activated Carbon and immobilized dominant bacteria in the said sodium alginate gel; Kernel is coated with chitosan, and chitosan is coated with the sodium alginate adventitia.
2. novel biology microcapsule preparation method who is applied to biological fluidized bed as claimed in claim 1 may further comprise the steps:
(1) preparation of microorganism mixed solution: in sodium alginate soln, add emulsifier tween 80 and Powdered Activated Carbon and stir obtaining solution A; Wherein the sodium alginate massfraction is 2.0%; Tween 80 concentration is 0.50%～1.0%, Powdered Activated Carbon 0.75% (W/V); In solution A, adding 100%～20% (V/V) advantage degradation germ liquid mixes and obtains solution B;
(2) solution B is added drop-wise to injection system through peristaltic pump in 4% the calcium chloride solution and reacts 15min～30min, make calcium alginate plastic beads;
(3) calcium alginate plastic beads that makes is filtered, and use sterile water wash; Place pH6.0,1.8% chitosan solution to react 10min～20min then and make alginate calcium/chitosan microcapsules;
(4) alginate calcium/chitosan microcapsules that makes is filtered the back sterile water wash; Place the sodium citrate solution liquefaction 5min-12min of 55mmol/L to make the microcapsule of kernel then for liquid state;
(5) microcapsule that will make filter the back and use sterile water wash, place 0.15% sodium alginate soln overlay film 10min～20min once more then, make bio-microcapsule;
(6) microcapsule are filtered and clean with pure water, then microcapsule being placed calcium ion solubility is that 0.05～0.1% physiological saline storage solution is preserved.
3. one kind like the described novel biology microcapsule preparation method who is applied to biological fluidized bed of claim 1～2; It is characterized in that the prepared microcapsule diameter is 3.0～4.0mm; Thickness is 10～20 μ m, and density is 1.05～1.08g/ml, and maximum machine intensity can reach 730.31g/cm
2, maximum molecular weight cut-off is about PEG4000, and relative molecular weight can freely pass through cyst membrane less than the small-molecule substance of PEG1500.
4. one kind like the application of the described novel biology microcapsule that is applied to biological fluidized bed of claim 1～2 on various organic wastewater with difficult degradation thereby are handled; It is characterized in that microcapsule are stable under acid and most of divalent cation condition; And monovalent cation and most of negatively charged ion are unfavorable for the stable of microcapsule; Biological fluidized bed aerating wastewater amount 80～140L/h, hydraulic detention time 2～3d.
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| CN201110183304.2A CN102351320B (en) | 2011-07-01 | 2011-07-01 | Method for preparing novel biological microcapsule for biological fluidized bed |
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| CN201110183304.2A CN102351320B (en) | 2011-07-01 | 2011-07-01 | Method for preparing novel biological microcapsule for biological fluidized bed |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102921359A (en) * | 2012-11-08 | 2013-02-13 | 山东轻工业学院 | Preparation method of biological capsule for sewage treatment |
| CN103275966A (en) * | 2013-06-04 | 2013-09-04 | 国家粮食局科学研究院 | Method for preparing coating microcapsule before microbial fermentation by utilizing common fermentation tank |
| CN103509781A (en) * | 2012-06-29 | 2014-01-15 | 中国科学院大连化学物理研究所 | Research model of bacterial bio-film |
| CN104030457A (en) * | 2014-05-21 | 2014-09-10 | 东莞市华中生物科技有限公司 | Method and fluidized bed for purifying eutrophic water by using microorganism filler |
| CN105296461A (en) * | 2015-11-17 | 2016-02-03 | 重庆泰克环保科技股份有限公司 | Microbial compound and preparation method and encapsulation pretreatment method thereof |
| CN105296460A (en) * | 2015-11-17 | 2016-02-03 | 重庆泰克环保科技股份有限公司 | Microbial capsule for wastewater treatment and preparation method thereof |
| CN105294262A (en) * | 2015-10-30 | 2016-02-03 | 全椒县琪悦家庭农场 | Nitrogen-fixing phosphate-dissolving potassium-dissolving microorganism compound fertilizer and preparation method thereof |
| CN105884040A (en) * | 2016-05-11 | 2016-08-24 | 长沙学院 | Preparation method of water body denitrification material |
| CN106430831A (en) * | 2016-10-28 | 2017-02-22 | 同济大学 | Bio-microcapsule-ultrafiltration membrane combined process for treating heavy metals and ammonia nitrogen in water |
| CN106490360A (en) * | 2016-10-31 | 2017-03-15 | 广州市义和化工有限公司 | One boar green feed additive and preparation method thereof |
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