CN106419837A - Fluorescent molecular tomography system based on spiral motivation - Google Patents
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- 238000003325 tomography Methods 0.000 title claims abstract description 26
- 230000008450 motivation Effects 0.000 title abstract 4
- 230000004927 fusion Effects 0.000 claims abstract description 20
- 238000000034 method Methods 0.000 claims abstract description 18
- 238000003384 imaging method Methods 0.000 claims abstract description 15
- 238000000799 fluorescence microscopy Methods 0.000 claims abstract description 3
- 239000007850 fluorescent dye Substances 0.000 claims description 21
- 238000004846 x-ray emission Methods 0.000 claims description 18
- 238000001506 fluorescence spectroscopy Methods 0.000 claims description 16
- 230000005284 excitation Effects 0.000 claims description 14
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- 238000013461 design Methods 0.000 description 1
- 238000002059 diagnostic imaging Methods 0.000 description 1
- 210000002216 heart Anatomy 0.000 description 1
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- 210000004185 liver Anatomy 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 210000002356 skeleton Anatomy 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
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- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0062—Arrangements for scanning
- A61B5/0064—Body surface scanning
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- A61B5/0033—Features or image-related aspects of imaging apparatus classified in A61B5/00, e.g. for MRI, optical tomography or impedance tomography apparatus; arrangements of imaging apparatus in a room
- A61B5/0035—Features or image-related aspects of imaging apparatus classified in A61B5/00, e.g. for MRI, optical tomography or impedance tomography apparatus; arrangements of imaging apparatus in a room adapted for acquisition of images from more than one imaging mode, e.g. combining MRI and optical tomography
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- A61B5/0071—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by measuring fluorescence emission
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Abstract
The invention discloses a fluorescent molecular tomography system based on spiral motivation. A motivation method on the existed point motivating light source system evenly distributed at one cycle is changed to be spiral motivation, so that a fluorescence probe is completely motivated, and the signal-to-noise ratio of the obtained image is largely improved. An X-ray tomography imaging system is combined with a fluorescence imaging system, and the obtained three-dimensional structure information is used as prior information of fluorescence rebuilding, and guided to a corresponding structure to present a correct optical parameter; finally, the fluorescence rebuilding result is matched to a three-dimensional structure by a computer so as to realize the multimoding information fusion of a matter to be imaged; thereby the correctness of the fluorescence rebuilding is improved.
Description
Technical field
The invention belongs to Medical Imaging, is related to a kind of in body toy fluorescent molecular tomography system design, tool
Say, be related to a kind of based on spirally exciting in body toy fluorescent molecular tomography system, the system can be used for glimmering body
Optical molecule tomographic data is gathered.
Background technology
Fluorescent molecular tomography (hereinafter referred to as FMT) is a kind of new image mode that developed recently gets up.It utilizes
External light source excites fluorescent probe（Fluorogen, fluorescent dye etc.）Making which launch photon, fluorescence is collected using fluorescent collecting device
Signal, combined mathematical module, it is possible to obtain fluorescent probe distributed in three dimensions in target.At present, existing disconnected in body toy fluorescence
Layer imaging device is many to excite fluorescent dye using an excitation source, and general shot point is to be evenly distributed in imageable target surface
One circle or several circles.Its weak point of mode of excitation of one circle of distribution be if equally distributed shot point and fluorescence are visited
Pin is highly too wide in the gap, and point excites the fluorescent probe that cannot ensure in thing to be imaged to be stimulated completely, and fluorescent probe is stimulated
The fluorescence data that not exclusively may result in collection is inaccurate, and acquisition signal pattern signal to noise ratio is low, and then three-dimensional reconstruction is forbidden
Really.The mode of excitation for being distributed several circles increases due to exciting to count out so that data acquisition time increases, it is impossible to meet clinically
Realtime imaging demand.
Content of the invention
The purpose of the present invention is the defect for overcoming above-mentioned mode of excitation, provide a kind of spirally excite in body toy
Fluorescent molecular tomography system, the system can improve the fluorescence data accuracy and signal to noise ratio for collecting.
In addition, we are also added in fluoroscopic imaging systems x-ray fault imaging mode for obtaining thing to be imaged
Three dimensional structure information, gives optical parametric according to this structure, thus rebuilds for fluorescent three-dimensional and provides structure priori and optical parametric
Priori.Finally using computer, the distributed in three dimensions of fluorescent probe is blended with three dimensional structure information, realize many of thing to be imaged
Modal information merges.
The concrete thought of the present invention is：On the existing point excitation light source system for being evenly distributed on a circle, will the side of exciting
Formula is changed to spirally excite, and so that fluorescent probe is stimulated completely, and the signal noise ratio (snr) of image of acquisition is greatly improved.X-ray tomography is become
As system is combined with fluoroscopic imaging systems, the prior information rebuild as fluorescence with its three dimensional structure information for obtaining, and draw
Leading and correct optical parametric is given to counter structure, finally using computer, fluorescence reconstructed results is matched real on three dimensional structure
The multimodal information fusion of existing thing to be imaged, improves the correctness of fluorescence reconstruction.
Its technical scheme is：
One kind in body toy fluorescent molecular tomography system, including near infrared laser, electrical-controlled lifting platform, automatically controlled rotation
Platform, thing to be imaged, narrow band filter slice, CCD camera, x-ray emission device, x-ray receptor, stereotype, computer, CCD camera and
Narrow band filter slice with exciter point-blank, and is in the opposite of laser instrument, and thing to be imaged is in CCD camera, arrowband filter
On the same straight line that wave plate, laser instrument are located, x-ray emission device is located on the direction vertical with exciter with x detector,
On the same line, laser instrument is on electrical-controlled lifting platform for x-ray emission device, thing to be imaged and x-ray detector, computer with
CCD camera, x-ray emission, electrical-controlled lifting platform are connected with electronically controlled rotary table：
The CCD camera, carries out transmission-type imaging for receiving fluorescence；
The narrow band filter slice, for filtering out other light in addition to fluorescence signal；
The electrical-controlled lifting platform, for controlling the height of laser instrument；
The electronically controlled rotary table, for driving thing to be imaged to be rotated, multi-angle gathers the data for projection of thing to be imaged, rotation
The rotation of platform is just constituted with the lifting of lifting platform and is spirally excited；
The exciter, for producing excitation source, is excited into and lights as the fluorescent probe of interior of articles, produce fluorescence signal；
The x-ray emission device, for launching x-ray；
The x-ray detector, for receiving x-ray；
The stereotype, for shielding damage of the x-ray to CCD camera camera lens；
The computer, for controlling CCD camera, electrical-controlled lifting platform, x-ray emission and detection, and electronically controlled rotary table operation,
Receive and process the fluorescence data for projection of CCD camera transmission, three-dimensional reconstruction is carried out to data for projection, the image after reconstruction is carried out
Registration fusion, receives and processes x-ray data, rebuild the structural information of object to be imaged, realize the multi-modal letter of thing to be imaged
Breath fusion.
The centre wavelength of the centre wavelength of described narrow band filter slice and fluorescence signal is consistent.
One kind is comprised the following steps in body toy fluorescent molecular tomography method：
（1）Acquisition fluorescence data is spirally excited
Computer controls lifting platform is in a certain height, and laser launched by laser instrument, and the CCD camera parallel with laser instrument is received and penetrated
The light of thing to be imaged forms transmissive projection data, completes projection data acquisitions；Electrical-controlled lifting platform adjustment laser instrument height
Degree, height size is by computer controls, while drive a certain angle of thing to be imaged rotation static by electronically controlled rotary table, angular dimension
By computer controls, repeat the process that previously described CCD camera gathers fluorescence data；Until thing to be imaged is rotated 360 degrees, glimmering
Light data collection terminates.Here, computer will control lifting platform and turntable synchronous operation, in the highly interior of lifting platform regulation
The rotation of to be imaged thing 360 degree is just completed.
（2）CT Ray Data Collection
Complete step（1）, computer controls x-ray emission device transmitting x-ray, the x-ray for penetrating thing to be imaged is visited through x-ray
Device detection is surveyed, computer completes CT projection data acquisitions, drive thing to be imaged to rotate by electronically controlled rotary table, complete 360 degree of scannings；
（3）CT data processing
Computer utilizes filter back-projection reconstruction algorithm, to step（2）The data for projection of handled mistake carries out three-dimensional reconstruction, obtains
The 3 D anatomy structure image of thing to be imaged；
（4）Fluorescence data is processed
CCD camera will collect data for projection and be transferred to computer, and computer is to step（1）The data for projection for being collected is carried out
Pretreatment；
（5）The three-dimensional reconstruction of fluorescence imaging
By step（3）Structural images as prior information, with algebraic reconstruction technique, three are carried out to pretreated data for projection
Dimension is rebuild, and obtains the distributed image of fluorescent dye；
（6）Three-dimensional reconstruction image registration fusion
Computer is to step（3）With（5）The three-dimensional reconstruction image registration fusion of two kinds of imaging patterns is acquired, is acquired fusion
Fluorescence faultage image afterwards, realizes the multimodal information fusion of thing to be imaged.
Step（2）In when carrying out post processing to CT data for projection, to each angle acquisition to data for projection will carry out
Process.
The present invention has advantages below：
First, the present invention excites fluorescent probe using spiraling manner, and overcoming can not in existing excitation source imaging system
Ensure that fluorescent probe is stimulated completely shortcoming, improves the signal to noise ratio of the fluorescence data of collection.
Second, the present invention excites fluorescent probe using spiraling manner, can improve fluorescence reconstructed results quality.
3rd, the fusion of fluorescent molecular tomography and two kinds of image modes of x-ray fault imaging is present invention employs, can
While obtaining three dimensional structure image and the fluorescent probe distribution situation of thing to be imaged, it is achieved that the information of two kinds of image modes is melted
Close, obtain the more biological characteristics of thing to be imaged.
Description of the drawings
Fig. 1 is the shot point for spirally exciting in the present invention（On thing to be imaged）Distribution schematic diagram；
Fig. 2 is structural representation of the present invention in body toy fluorescent molecular tomography system；
Fig. 3 is flow chart of the present invention in body toy fluorescent molecular tomography method.
Fig. 4 is reconstruction result schematic diagram, wherein（a）-（c）Figure is the fluorescent target fluorescent yield Distribution value figure in section；
（d）-（f）Figure is shown as the three-dimensional display of true fluorescent target and the representative fluorescent target that rebuilds.
Specific embodiment
Specific embodiment is describing technical scheme in detail below in conjunction with the accompanying drawings.
Referring to Fig. 1, fluorescent molecular tomography, it is to irradiate thing A to be imaged using outside visible ray for excitation source, this
Bright shot point B on thing A to be imaged helically formula distribution.
With reference to Fig. 2, the present invention in body toy fluorescent molecular tomography system, including near infrared laser 1, automatically controlled liter
Drop platform 2, electronically controlled rotary table 3, thing to be imaged 4, the narrow band filter slice 5 for filtering exciting light, CCD camera 6, x-ray emission device 7, x are penetrated
Line receiver 8, stereotype 9, computer 10.
Fluorescent molecular tomography, is to irradiate thing to be imaged using outside visible ray for excitation source, and now more should
It is imaged for living small animal, particularly mice imaging.Before fluorescent molecular tomography is carried out, treated with fluorescent probe labelling
Imaging thing.
Computer 10 controls lifting platform 2 and turntable 3 motionless, and laser launched by laser instrument 1, irradiates thing 4 to be imaged.To be imaged
The fluorescent probe of the prior labelling of thing 4 is irradiated by exciting light, sends the light different from excitation wavelength, i.e. launching light.Transmitting
Light penetrates thing to be imaged 4, filters out other light in addition to launching light through narrow band filter slice 5, and CCD camera 6 receives transmission
The launching light for going out produces fluorescence data for projection.The data for projection of generation is transferred to computer 10 by CCD camera 6.
X-ray launched by x-ray emission device 7, penetrates thing to be imaged 4 and is detected by x-ray detector 8, and the x-ray of generation is thrown
Shadow data transfer is to computer 10.In order to shield damage of the x-ray to CCD camera camera lens, pass through in CCD camera 6 and x-ray
Stereotype 9 is provided between region.
X-ray emission device 7, thing to be imaged 4 will be on the same line with x-ray detector 8.
The centre wavelength of narrow band filter slice 5 will be consistent with the centre wavelength of fluorescence signal.
Below in conjunction with the accompanying drawings 3, the method for the present invention is described further.
（1）Fluorescence data is gathered：
Computer 10 controls lifting platform 2 and turntable 3 motionless, and laser instrument 1 is launched laser and irradiates thing 4 to be imaged, and fluorescent probe is received
Excitation-emission fluorescence signal, penetrates thing to be imaged 4 together with exciting light, through narrow band filter slice 5 filter out except fluorescence signal with
Other outer light, CCD camera 6 receives the fluorescence signal after filtering and produces fluorescence data for projection.Initial position data is gathered
Afterwards, electrical-controlled lifting platform 2 adjusts 1 height of laser instrument, while electronically controlled rotary table 3 drives thing to be imaged 4 to be rotated, each anglec of rotation
Degree can be set as a certain fixed value as needed, but must ensure that lifting platform is rotated in the highly interior just turntable 3 of regulation
Complete tour.It is achieved in spirally being excited into picture.
（2）CT data acquisition：
X-ray launched by x-ray emission device 7, penetrates thing to be imaged 4 and is received by x-ray detector 8, produces x-ray projection data,
While electronically controlled rotary table 3 drives thing to be imaged 4 to rotate, x-ray projection data is completed after thing to be imaged 4 rotates a circle and adopt
Collection, and data for projection is transferred to computer 10.
（3）CT data reconstruction：
Computer 10 utilizes filter back-projection algorithm, carries out three-dimensional reconstruction to the handled x-ray projection data that crosses of step 2, obtains
Structural images to thing to be imaged 4.
（4）Fluorescence data three-dimensional reconstruction：
By step（3）The CT reconstructed results for obtaining are asked with algebraic reconstruction technique as the prior information of fluorescence data three-dimensional reconstruction
Solution obtains the distributed image of fluorescent probe in thing 4 to be imaged.
（5）Three-dimensional reconstruction image registration fusion：
Computer 10 is to step（3）With（4）Three-dimensional reconstruction result carry out image registration fusion, obtain the fluorescence tomography after fusion
Imaging, it is achieved that the multimodal information fusion of thing to be imaged.
The above, the only present invention preferably specific embodiment, protection scope of the present invention not limited to this, any ripe
Those skilled in the art are known in the technical scope of present disclosure, the letter of the technical scheme that can become apparent to
Altered or equivalence replacement are each fallen within protection scope of the present invention.
With reference to Fig. 4, the reconstructed results of the present invention are further described.
Phantom places one mainly comprising muscle, skeleton, heart, kidney and five tissues of liver wherein in right lung
Cylinder is used as fluorescent target, and its real center position is (6,0,0) mm.Figure（a）With（b）Respectively shot point is evenly distributed on
Reconstructed results at z=5mm and z=-5mm（As a result it is the fluorescence distribution in z=0mm section）.Figure（c）Obtain for spiral shot point
Result section z=0mm fluorescent yield Distribution value, figure（d）-（f）For corresponding three-dimensional display, in figure darkviolet region
For reconstructed results.Mode of excitation based on the present invention can make fluorescent target be stimulated completely, and achieve two kinds of image modes
Information fusion, obtains the more biological characteristics of thing to be imaged.
Claims (7)
1. a kind of based on the fluorescent molecular tomography system for spirally exciting, including near infrared laser, electrical-controlled lifting platform, electricity
Control turntable, thing to be imaged, narrow band filter slice, CCD camera, x-ray emission device, x-ray receptor, stereotype, computer, CCD
The camera lens of camera faces laser instrument, is respectively provided at the both sides of thing to be imaged, is provided with narrow band filter slice before the camera lens of CCD camera,
CCD camera, thing to be imaged, CCD camera and narrow band filter slice are point-blank；X-ray emission device is located at x detector and swashs
Send out on the vertical direction of device, x-ray emission device and x-ray detector are located at thing both sides to be imaged, and three is in a straight line；Laser
Device is located on electrical-controlled lifting platform, and computer is connected with CCD camera, x-ray emission, electrical-controlled lifting platform and electronically controlled rotary table.
2. according to claim 1 based on the fluorescent molecular tomography system for spirally exciting, it is characterised in that described
CCD camera carries out transmission-type imaging for receiving fluorescence；The narrow band filter slice be used for filtering out in addition to fluorescence signal its
His light；The exciter is used for producing excitation source, is excited into and lights as the fluorescent probe of interior of articles, produces fluorescence letter
Number；The x-ray emission device is used for launching x-ray；The x-ray detector is used for receiving x-ray；The stereotype is used for shielding
Damage of the x-ray to CCD camera camera lens；The computer is used for controlling CCD camera, electrical-controlled lifting platform, x-ray emission and spy
Survey, and electronically controlled rotary table operation, receive and process the fluorescence data for projection of CCD camera transmission, three-dimensional is carried out to data for projection
Rebuilding, registering fusion carried out to the image after reconstruction, receives and process x-ray data, rebuild the structural information of object to be imaged,
Realize the multimodal information fusion of thing to be imaged.
3. according to claim 1 based on the fluorescent molecular tomography system for spirally exciting, it is characterised in that described
The centre wavelength of narrow band filter slice and the centre wavelength of fluorescence signal be consistent.
4. according to claim 1 based on the fluorescent molecular tomography system for spirally exciting, it is characterised in that described
Electrical-controlled lifting platform can be so that mode of excitation be changed into and spirally excites with work while electronically controlled rotary table.
5. a kind of based on the fluorescent molecular tomography method for spirally exciting, comprise the following steps：
（1）Acquisition fluorescence data is spirally excited：
Computer controls lifting platform is in a certain height, and laser launched by laser instrument, and the CCD camera parallel with laser instrument is received and penetrated
The light of thing to be imaged forms transmissive projection data, completes projection data acquisitions；Electrical-controlled lifting platform adjustment laser instrument height
Degree, height size is by computer controls, while drive a certain angle of thing to be imaged rotation static by electronically controlled rotary table, angular dimension
By computer controls, repeat the process that previously described CCD camera gathers fluorescence data；Until thing to be imaged is rotated 360 degrees, glimmering
Light data collection terminates；Computer controls lifting platform and turntable synchronous operation, in highly interior just the completing of lifting platform regulation
The rotation that 360 degree of thing to be imaged；
（2）CT Ray Data Collection：
X-ray launched by computer controls x-ray emission device, and the x-ray for penetrating thing to be imaged is detected through x-ray detector, meter
Calculation machine completes CT projection data acquisitions, drives thing to be imaged to rotate by electronically controlled rotary table, completes 360 degree of scannings；
（3）CT data processing：
Computer utilizes filter back-projection reconstruction algorithm, to step（2）The data for projection of collection carries out three-dimensional reconstruction, obtains and treats into
3 D anatomy structure image as thing；
（4）Fluorescence data is processed：
CCD camera will collect data for projection and be transferred to computer, and computer is to step（1）The data for projection for being collected is carried out
Pretreatment；
（5）The three-dimensional reconstruction of fluorescence imaging：
By step（3）Structural images as prior information, with algebraic reconstruction technique, three are carried out to pretreated data for projection
Dimension is rebuild, and obtains the distributed image of fluorescent dye；
（6）Three-dimensional reconstruction image registration fusion：
Computer is to step（3）With（5）The three-dimensional reconstruction image registration fusion of two kinds of imaging patterns is acquired, is acquired fusion
Fluorescence faultage image afterwards, the distributed image different modalities of the 3 D anatomy structure image and fluorescent dye of realizing thing to be imaged are believed
The fusion of breath；
Step（2）In when carrying out post processing to CT data for projection, to each angle acquisition to data for projection will process.
6. according to claim 5 fluorescent molecular tomography method is spirally excited, it is characterised in that step（1）In
Described electrical-controlled lifting platform need to adjust laser height, while driving the thing anglec of rotation to be imaged, Liang Zhepei by electronically controlled rotary table
Conjunction forms spiral mode of excitation.
7. according to claim 5 in body toy fluorescent molecular tomography method, it is characterised in that step（5）In to fluorescence
When data are rebuild, step is make use of（3）The 3 D anatomy structure image for being obtained is prior information, is conducive to improving fluorescence
The quality of image reconstruction.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107242860A (en) * | 2017-07-25 | 2017-10-13 | 京东方科技集团股份有限公司 | Fluorescent molecular tomography system and method |
| CN108836275A (en) * | 2018-07-10 | 2018-11-20 | 天津工业大学 | A kind of fluorescent molecular tomography system under free space |
| CN110520689A (en) * | 2017-04-21 | 2019-11-29 | 株式会社岛津制作所 | The length-measuring error evaluation utensil of 3-d shape measurement X ray CT device |
| CN111103275A (en) * | 2019-12-24 | 2020-05-05 | 电子科技大学 | PAT prior information assisted dynamic FMT reconstruction method based on CNN and adaptive EKF |
| CN112168123A (en) * | 2020-10-15 | 2021-01-05 | 山东艾博康生物科技有限公司 | Oral cavity detector for data acquisition and transmission |
| CN114468998A (en) * | 2022-01-26 | 2022-05-13 | 西安电子科技大学 | Single-view-angle reflection type near-infrared two-region fluorescence dynamic tomography system and method |
Citations (3)
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| CN108836275A (en) * | 2018-07-10 | 2018-11-20 | 天津工业大学 | A kind of fluorescent molecular tomography system under free space |
| CN111103275A (en) * | 2019-12-24 | 2020-05-05 | 电子科技大学 | PAT prior information assisted dynamic FMT reconstruction method based on CNN and adaptive EKF |
| CN111103275B (en) * | 2019-12-24 | 2021-06-01 | 电子科技大学 | PAT prior information assisted dynamic FMT reconstruction method based on CNN and adaptive EKF |
| CN112168123A (en) * | 2020-10-15 | 2021-01-05 | 山东艾博康生物科技有限公司 | Oral cavity detector for data acquisition and transmission |
| CN114468998A (en) * | 2022-01-26 | 2022-05-13 | 西安电子科技大学 | Single-view-angle reflection type near-infrared two-region fluorescence dynamic tomography system and method |
| CN114468998B (en) * | 2022-01-26 | 2023-06-30 | 西安电子科技大学 | Single-view angle reflection type near infrared two-region fluorescence dynamic tomography system and method |
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