CN100469324C - Data acquisition system for nuclein and fluorescent dual module integral small animal molecules imaging - Google Patents
Data acquisition system for nuclein and fluorescent dual module integral small animal molecules imaging Download PDFInfo
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- CN100469324C CN100469324C CNB2007100026711A CN200710002671A CN100469324C CN 100469324 C CN100469324 C CN 100469324C CN B2007100026711 A CNB2007100026711 A CN B2007100026711A CN 200710002671 A CN200710002671 A CN 200710002671A CN 100469324 C CN100469324 C CN 100469324C
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Abstract
The invention belongs to the field of application of near-infrared laser, nuclear irradiation, electron and image rebuild in systematic biology and medicine. It is characterized in that high-specificity nuclear species and fluorescent probe are implanted in live animal to realize double mark of muscle deep layer or internal organ tumor cell. The emission signal of nuclear species is checked by sensitive electron-multiplier phototube in array copulation position of scintillation crystal; feeble fluorescent signal generated by visible light or infrared agitation is checked by high quantum yield CCD camera. The two checking systems are orthogonally arranged in the same surface, the live nuclear species and fluorescent signal can be checked simultaneously through rotary image cavity, and double module three dimensional tomography image of small animal can be rebuilt with software algorism. The invention is characterized by multi-image forming modules, abundant information and simple operation.
Description
Technical field
The data-acquisition system of the molecular imaging of the toy of nucleic and fluorescent dual module integral belongs to laser, the nuclear radiation, and electronics and image reconstruction are in the complex art field of systems biology and medical application.
Background technology
The invention belongs to one of molecular image application technology.After the medical image technology has experienced structure imaging, functional imaging, the nineties in last century, along with biological and the development of gene technology and the raising of imaging technique, new molecular image technology has appearred again.It can realize the noinvasive of organism internal physiological or pathological process, the imaging in vivo of dynamic real-time on gene, cell and molecular level, thereby provides effective information to obtain and the analyzing and processing means for researchs such as the mechanism of action of disease related gene functional localization, cell growth promoter and mutation process, new drug development, oncotherapys.At present because the molecular image technology in the deficiency of aspects such as resolution, penetration depth and safety, mainly is applied to the toy imaging in vivo, as an important tie that carries out the transition to clinical practice from isolated experiment.The molecule image system development is mainly launched by two approach with exploitation, first utilization has been applied to clinical comparatively sophisticated imaging technique, mainly be the radio nuclide imaging system, as positron emission tomography technology (PET) and single photon emission tomographic imaging technology (SPECT).They pass through radioactive nucleus metabolic some chemical compound of labelling participation organism usually, the electronics of its positron that sends and organism self combines then, effect is buried in oblivion in generation, producing two energy is the opposite γ photon of the 511MeV direction of motion, determines the CONCENTRATION DISTRIBUTION of specific compound by vitro detection γ photon.An other approach is the new imaging in vivo method of exploitation, mainly is optical imagery, comprises fluorescence imaging or organism self-luminous imaging and corresponding tomography technology.Optical imagery utilizes luciferase or all kinds of existing fluorescent probe of using in the section experiment of exsomatizing to come the interested gene of labelling, cell and molecule, the fluorescence information of utilizing body surface or vitro detection to arrive then, according to certain light propagation model, reconstruct institute's marker gene, cell and molecule scattergram in vivo.The fluorescence fault imaging is owing to the optical density that fluorescent probe sends can be controlled by exciting light, so at imaging depth, the contrast aspect is better than the self-luminous imaging.
As mentioned above, two technology of radio nuclide imaging and fluorescence imaging all can be obtained the information of molecule and cellular level, all have sophisticated product to occur at present separately.Wherein, from 1972 first positron emission tomography technology (PET) occur and be applied to clinical since, external medical apparatus and instruments giant enterprise such as Siemens, GE, Philips etc. have comparatively sophisticated PET product, and the system PET-CT of released one after another PET and the fusion of X-ray fault imaging, and the integration technology of PET and nuclear magnetic resonance (MRI) is also just in the middle of commercialization, and their advantage all is to remedy the deficiency of independent PET on imaging space resolution.For toy fluorescence imaging in vivo system, the specialize toy the U.S. Xenogen company of bulk optics imaging also be done self-luminous imaging for many years after, the product that is used for fluorescence imaging has just been released in beginning in nearest 1 year successively.But it is paid much attention to this field, just releases the fluorescence computed tomography (SPECT) system that can carry out at body toy 360 degree panoramas in 05 year, is the unique a kind of product that can carry out the whole fault imaging of toy in the present whole world.
Yet domestic corporation does not but have the product of small animal position emission tomography (PET) imaging system or optical imaging system as yet, just has research institution of several family to carry out relevant basic research and has developed model machine.Wherein, the high the subject of knowledge and the object of knowledge of the Chinese Academy of Sciences is developed domestic first single and four row PET people volume imaging equipment research, and develops the small animal position emission tomography (PET) of resolution less than 2mm in the near future; The optical imagery aspect, some contributions are not only made at diffuse optical fault imaging theoretical side by Tsing-Hua University.Solved the border issue that light transports in biological tissue, foretold boundary condition first, and successfully developed the diffuse optical imaging system and the toy fluorescence computed tomography (SPECT) system blank of tissue the anisotropic dependency of microcosmic scattering.
The continuous progress of accompanying image treatment technology and the development of various imaging means, multi-modality imaging become a developing direction of following imaging system.The product of existing a lot of double-mode imaging system of present American-European medical apparatus and instruments enterprise, as PET and CT combination, the bonded system of optical imagery and CT, their advantage can pass through registration with high-resolution multi-modality images, cut apart and merge, realize high-precision space orientation.Merging formation new image information source also can provide more comprehensive information, for research worker is offered help.
Yet, for the fluorescence computed tomography (SPECT) system that has just grown up, just retrieve domestic and international patent, up to the present adopt the dual mode system of nucleic and fluorescence imaging that report is not arranged in the world wide.Comparatively approaching is optics of molecular image research group (Crump Institute for Molecular Imaging) proposition of Univ California-Los Angeles USA and the scheme that position emissron tomography instrument (PET) combines, and called after Optical-PET (OPET), this scheme is transformed the scintillation crystal that PET uses, crystalline two ends are opened, make it to receive high-energy gamma ray, again can transmitting optical signal.Simultaneously also photomultiplier tube has been done suitable improvement, made it to have higher quantum efficiency to improve optical detection sensitivity at visible light wave range.Though this scheme can realize bimodal and detect, and measures but this system only can realize the animal body self-luminous, does not have the function of fluorescence imaging.And, be planar imaging, can not carry out the fault imaging of toy integral body.In addition, this system needs special photomultiplier tube price more expensive, and the coupling of crystal and photomultiplier tube is difficulty comparatively also, and the required electronic system of the separation of two kinds of signals is also quite complicated.System compares therewith, and the present invention realizes the detection scheme of partition space by adopting the rotation toy, has clear in structure, is convenient to realize that post-processed is simple that autgmentability waits remarkable advantage by force.
Summary of the invention
The objective of the invention is to, set up the molecular imaging system of the toy of a nucleic and fluorescent dual module integral.
The invention is characterized in, the small animal molecule image-forming system of described nucleic and fluorescent dual module integral, contain: cylinder imaging chamber, nucleic detect imaging system, fluoroscopic examination imaging subsystems and computer, the lateral surface in this imaging chamber and in addition part two plane cuttings being orthogonal to described imaging chamber central axis be uniform four, described nucleic detects imaging subsystems and respectively accounts for two 1/4 relative measurement spaces with the fluoroscopic examination imaging subsystems, wherein:
Cylinder imaging chamber, contain: sidewall has the cavity main body of fluorescence excitation window respect to one another and fluoroscopic examination window, the support of the suspension toy that is positioned at intracavity and can again can in the vertical direction moves at the horizontal plane internal rotation, and link to each other with described support and can control support and horizontally rotate motor with vertical motion.This motor operates at following any rotation mode: revolve the switching spatial mode that turn 90 degrees, per step at every turn and rotate a low-angle step one and adopt type and at the uniform velocity rotation, adopt type while changeing, so that make described nucleic detection imaging subsystems and fluoroscopic examination imaging subsystems carry out the panorama data acquisitions of 360 degree to the toy whole body.
Nucleic detects imaging subsystems, contain: be evenly distributed on scintillation crystal bar and location-sensitive photomultiplier tube in outer two the 1/4 relative measurement space scopes of this imaging chamber lateral surface more than one, in order to detect the paired γ photon that nucleic sent that injects living small animal body internal labeling particular organization and cell.γ light is converted to visible light through scintillation crystal, is converted to the signal of telecommunication through photomultiplier tube again.
The fluoroscopic examination imaging subsystems, contain: laser instrument, photoswitch and excitation fiber are arranged in of two 1/4 relative spaces in addition beyond this imaging chamber lateral surface; Have at its 1/4 relative measurement space: receive optical fiber, protection stereotype group, dash receiver and optical filter, camera lens and CCD (Charge Coupled Device (CCD)), wherein:
Excitation fiber and reception optical fiber, each is made of the multichannel multimode fibre, and exciting light enters described imaging chamber by excitation fiber from the fluorescence excitation window.Receive optical fiber and be close to affiliated fluoroscopic examination window from surface, imaging chamber diverse location receiving optical signals.The fluorescence that this signal excites the back to be sent from the fluorescent probe stimulated luminescence that injects living small animal body internal labeling specific molecular or cell.
Photoswitch is a multi-channel optical fibre switch, and computer is realized the switching of multi-path laser source by the programme-control switching channel, and every road optical signal is coupled to this imaging chamber via excitation fiber.
Laser instrument is for selected photoswitch provides the fluorescence excitation light source.
Protection stereotype group, be positioned at described CCD camera lens before, area is avoided the high energy gamma photon damage CCD that overflows from toy tissue at least greater than the CCD visual field.
Computer, input signal are from through the output signal of telecommunication of photomultiplier tube with from the output signal of described CCD device, and output signal is rotation control and the switching signal that is controlled to picture intracavity motor.At this moment, also have, and calculate data image signal according to algorithm for reconstructing to the control signal of CCD camera with to the passage switch-over control signal of photoswitch.
Effect of the present invention is, in conjunction with radio nuclide imaging and fluorescence imaging advantage separately, sets up one and realizes that simply lower, the informative bimodal imaging system of cost cooperates algorithm for reconstructing and image interfusion method, finally can generate the bimodal faultage image.Can observe different material with radionuclide and fluorescent probe labelling so on the one hand simultaneously at intravital changes in distribution situation of toy and mutual relation, and can utilize its priori that provides mutually to improve the image reconstruction quality; On the other hand, the isotope labeling deep tissues that available penetrance is strong, more shallow organizing then can be used the fluorescent probe labelling, thereby can reduce nucleic dosage, reduces the radiation injury to organism.In the future, utilize this technology and pertinent instruments to set up a toy at integrated general detection technique platforms of integral system such as the research of body molecular imaging, medical applications and drug screenings, also can carry out the work of aspects such as physiological data processing, image reconstruction, image co-registration and three-dimensional visualization on this basis, lay the foundation at real application research such as the tumor-localizing of body, cancerous cell diffusions for carrying out toy in a deep going way.
Description of drawings
Fig. 1 is the fundamental block diagram of imaging system of the present invention.
Fig. 2 is the structural plan of the branch space double-mode imaging of imaging system of the present invention.
Fig. 3 is the schematic diagram of imaging system of the present invention, and the implementation of PET imaging and fluorescence imaging in the dual mode system is described.
Fig. 4 is the spectral characteristic of used fluorescent probe CY5.5 and optical filter XF3113 (710AF40) in the present embodiment: heavy line is represented the penetrating rate of optical filter, and dotted line "---" is represented the Cy5.5 excitation spectrum, and fine line is represented the Cy5.5 fluorescence spectrum.
Fig. 5 is the vertical view of the stereotype group design of required protection CCD in shared 1/4 space of fluorescence detection part.
Fig. 6 is the dual module integral scheme of PET and archebiosis light fault imaging for this reason, and the easy expansion of this dual mode system has been described.
The specific embodiment
Double-mode imaging system described in the present invention mainly comprises following four basic steps, 1) the labelling preparatory stage.Labeled cell or tissue will be used in specific nucleic and the fluorescent marker injection living small animal body.2) tested animal is fixed.Certain reaction and after the gathering time with toy anesthesia and hang on the support of imaging intracavity, needs its extremity are fixed simultaneously.Intracavity can be filled optical medium liquid (as fat milk solution) as required.3) data acquisition phase.The support that animal hung is made low-speed rotation by the step motor control of imaging cavity bottom around vertical direction, at this moment, be positioned at the nucleic detector and the fluorescence detector difference acquired signal that are orthogonal and distribute around the imaging chamber, initial data is sent into computer through the control interface card.
Imaging system of the present invention is characterised in that:
1) adopted branch space detecting pattern.With the lateral surface in cylinder imaging chamber and in addition part two plane cuttings being orthogonal to central axis be uniform four.Equal according to wanting detected magnitude in the PET imaging, the needs of the paired photon that direction is opposite, the emission of PET imaging detection and fluorescence imaging detects and respectively accounts for two 1/4 relative measurement spaces.Simultaneously, overflow the high energy gamma photon and damage the CCD that detects the low light level for preventing border, fluorescence measurement territory, the fluorescence signal of drawing on the discrete point of surface, imaging chamber with optical fiber passes custom-designed protection stereotype group again.Like this, two kinds of imaging pattern independent parallels carry out, and can obtain two kinds of imaging information needed simultaneously, system works efficient is high and two inter-signal interference are less, post-processed is simple, has very high reliability, also can significantly reduce the discordance of later image fusion treatment aspect.，
2) by support at horizontal plane internal rotation suspension toy, and the in the vertical direction traversing carriage, can realize the panoramic scannings of whole three-dimensional 360 degree of toy are increased known data boundary amount.Rotate differently with the signals collecting mode, the data volume that obtains is also different.Can adjust rotation and sample mode according to measuring needs.As can there be following three kinds of modes (a) to revolve the switching spatial mode that turn 90 degrees at every turn, (b) per step is rotated a low-angle, and a step one is adopted type, and (c) type is adopted at the uniform velocity rotation while changeing.Be explained as follows in detail: (a) from initial state, support whenever revolves and turn 90 degrees, and stops image data once.Can finish the 360 degree panorama data acquisitions of exciting light and detector for fluorescence mode like this to the toy whole body, but the density of data acquisition is constant, be the density that optical fiber is arranged in the outside, imaging chamber, just the measurement space with respect to tested toy has switched a week.For the PET imaging, then can repeat to adopt every group of test surface data twice vis-a-vis, can utilize the two secondary data reconstructed image of averaging.Perhaps only adopt once.(b) from initial state, low-angle of the every rotation of support, stop and adopt sample one time, tested like this toy constantly changes with respect to the relative position of fluorescence source or detector, finally luminous energy is not realized 360 degree panorama samplings, also be equivalent to increase fluorescence source or detector density, thereby increased the given data amount.For the PET detector also is similar.(c) low speed at the uniform velocity rotates, the image data while rotating, if image data is enough fast, tested toy does not change in gatherer process with respect to position of detector in the time of can regarding collection as.The low speed benefit of at the uniform velocity rotating is that toy is in a metastable state on the one hand like this, to its physiological process to influence meeting very little.On the other hand, motor also be easy to control, and can adopt multi-group data, be equivalent to improve sampling density.
3) adopt this minute tested dual mode system building mode of space rotation, two kinds of parallel finishing of imaging process, system flexibility is strong, can realize the combination of disparate modules.Can realize organism self-luminous fault imaging and diffused light fault imaging etc. as the fluorescence imaging part through simple change.System just can adapt to different detection demands like this, and the utilization by interactive information, and imaging parameters also can increase.
As previously mentioned, the invention provides a kind of flexible practicality, the imaging system of nucleic and fluorescent dual module integral.Fig. 1 is the population structure block diagram of this bimodulus small animal molecule image system among the present invention.This shows that system body has four parts to constitute, and is respectively computer, small animal imaging chamber, nucleic detector, fluorescence detector.How to realize the visible Fig. 2 of nucleic fluorescent dual module integral.
System adopts the parallel independent imaging that divides spatial measurement pattern to realize PET and fluorescence imaging.Fig. 2 illustrates this minute space measurement, the method for tested animal low speed rotation with the vertical view in the present embodiment.Columniform small animal imaging chamber is the core component of this system.Cavity main body is made by the black matt material, and is watertight, as the acetal resin Delrin series of Du Pont.Cavity wall has fluorescence excitation window respect to one another and fluoroscopic examination window.Fluorescence excitation and receive unaffectedly like this, the light of other boundaries then major part is absorbed, thereby is easy to influencing each other between the setting of boundary condition and the minimizing system.And the positive neutrality in imaging chamber has rotatable animal support, the speed of rotation by the chamber at the bottom of Electric Machine Control.
Imaging chamber lateral surface and in addition part two plane cuttings being orthogonal to central axis be uniform four (a), (b), (c), (d).Wherein the paired high-energy photon of PET imaging detects and accounts for (a), (c) two 1/4 relative measurement spaces, and the emission of fluorescence imaging detects and accounts for (b), (d) two 1/4 measurement space.
Detect for PET, at (a), (c) device that is used for being detected as the γ photon in two spaces is duplicate.At first be to be converted to visible light by (1) scintillation crystal, scintillation crystal adopts LSO in the present embodiment, because LSO has very high photo-quantum efficiency, can obviously reduce counting at random, reduces the image scattering.LSO crystal emission light is converted into the signal of telecommunication by (2) location-sensitive photomultiplier tube and sends into Computer Processing.
In (b) at fluorescence imaging place, (d) two spaces, (b) space takies for the multichannel excitation source.Excite flow process as follows: at first (3) laser instrument sends a branch of light corresponding to the fluorescent probe excitation wavelength, is divided into several (being 8 tunnel in the present embodiment) via (4) photoswitch then, delivers to surface, imaging chamber by (5) excitation fiber.(d) space is a measurement space, at first receives optical fiber by (6) of being close to imaging chamber outer surface and the fluorescence that receives or exciting light are drawn passes one custom-designed (7) protection stereotype group and arrive (a 8) dash receiver that is used for inserting the marshalling of optical fiber.The signal that last optical fiber is drawn arrives CCD via (9) optical filter, camera lens, and finally the form with image is delivered to computer.
In order to obtain the measurement image of tested toy 360 degree panoramas, this dual mode system adopts and allows toy hang on to be positioned on the support of imaging top of chamber.Support is horizontally rotated and vertical motion by step motor control, thereby can obtain the PET and the required measurement data of fluorescence fault imaging of toy whole body 360 degree.Can adjust different rotation modes and signals collecting mode according to measuring needs.Per step of switching spatial mode (b) that rotation mode has (a) to revolve at every turn and turn 90 degrees is rotated a low-angle, and a step one adopts type (c) and at the uniform velocity rotates, and adopts type while changeing.Present embodiment at first takes (a) kind to revolve the metering system that turn 90 degrees at every turn.
Fig. 3 is the structure principle chart of system of the present invention, be actually in each module of Fig. 1 dual mode system and done detailed explanation, and the signal flow of PET imaging and fluorescence imaging in the dual mode system has been described respectively.Mainly comprise fluorescence excitation in the fluorescence imaging module and detect two parts.Fluorescent material sends the wavelength atomic hypofluorescence signal longer than exciting light after being subjected to exciting in the animal body, and fluorescence passes toy physiological tissue and detected by photoelectric device.In excitation portion,, adopt narrow line spectrum high stability laser instrument (1) as the fluorescence excitation light source for effectively suppressing exciting light and extracting fluorescence signal.Take the VA671-200 laser instrument (operation wavelength 671nm, fiber power are that 0～100mW is adjustable) of Chinese VIASHO company in the present embodiment, because adopt Cy5.5 as fluorescent marker.Output connects the geometric ratio beam splitter (2) of a single input dual output, and one of them output arrives measuring junction through reference optical fiber (3), is used to provide one to eliminate the light intensity signal jitter error, realizes the reference optical signal of measurement update.Another output connects photoswitch (4) (multi-channel optical fibre switch, the SUN-FSW1X16MM of Chinese SUN company), realize the multichannel excitaton source by the programme-control switching channel, and every road optical signal (5) is coupled to small animal imaging chamber (6) by multimode fibre.In the present embodiment, any time only has a source to transmit, also can be by the different excitation source position grouping of programme-control.
The fluoroscopic examination end is mainly drawn received signal (7) by the reception optical fiber of being close to lateral surface diverse location place, imaging chamber.In 1/4 fluorescence measurement space, draw in the present embodiment and be positioned at same horizontal plane, equidistant 8 road optical fiber of arranging.Avoid tissue to overflow the high energy gamma photon and damage CCD, before the CCD camera lens, need to place a custom-designed protection stereotype group (8) that stops the γ photon.Protection stereotype area is greater than the CCD visual field at least.Through behind the stereotype optical fiber being caused the dash receiver of a perforate.The diffused light that see through dash receiver this moment comprises exciting light and fluorescence two parts, accurately record fluorescence signal, must be by bandpass filter (9) filtering exciting light and external interference light.Adopt the XF3113 (710AF4O) of U.S. OMEGAOPTICAL company to come filtering interference signals in the present embodiment.Fig. 4 is the spectral characteristic of fluorescent probe CY5.5 and optical filter XF3113 (710AF40); The crucial exploring block of fluorescence comprises camera lens and CCD camera (10), and camera lens has large-numerical aperture, and the external recirculated water refrigerator of CCD camera reduces the chip operation temperature, reduces dark current noise.CCD adopts the DU-897.CCD camera of Britain Andor company and the information interaction of main frame to be finished by the controller of a pci interface in the present embodiment, and the signal that CCD collects carries out image reconstruction after being input to computer, determines the three-dimensional position of fluorescent probe.
It is right that the nucleic detector is mainly used in detection high energy gamma photon, it buries in oblivion the phenomenon generation by positron and the electronics in the histoorgan that the nucleic decay forms, detector is measured (11) the γ photon that flies dorsad, scintillator crystal materials (12) the LSO crystal emission light that adopts is converted into the signal of telecommunication by location-sensitive photomultiplier tube (13), the temporal information of the energy signal of photon and due in need be sent into front end electronics jointly amplify and meet in the system (14) and go, output signal is through suitably sending into computer (15) processing by acquisition system after the conditioning.The PET detection technique is comparatively ripe, no longer describes in detail here.
On optics and nucleic signal detection basis, can finish the multi-modal date processing of living small animal, reconstruction, fusion, and realize three-dimensional visualization.The instrumentation that the present invention relates to is unified to coordinate by computer, and host software is finished equipment control, and data collection and analysis, image recover and rebuild.Good man computer interface also provides maximum convenience for the user, is ray machine electricity one height integrated products.Experiment showed, this invention stable performance, the automaticity height has quite good detecting sensitivity and image resolution ratio, accomplishes the end in view.
Fig. 4 is the luminous spectrum that uses in the present embodiment spectral characteristic at the fluorescent probe Cy5.5 and the optical filter XF3113 (710AF40) of near infrared band, and this is also commonly used in the fluorescence fault imaging.Among the figure: heavy line is represented the penetrating rate of optical filter, and dotted line "---" is represented the Cy5.5 excitation spectrum, and fine line is represented the Cy5.5 fluorescence spectrum
Fig. 5 is the vertical view of the stereotype group design of required protection CCD in shared 1/4 space of fluorescence detection part.In order to prevent that the high energy gamma photon from damaging CCD, arrange to absorb the γ photon with three stereotype dislocation that have the aperture that passes through for the reception fibre bundle.Aperture position is staggered in order to prevent to have in the aperture γ photon to reveal.
Fig. 6 has illustrated the expansibility of this PET and fluorescence imaging dual mode system.As long as corresponding (d) fluorescence measurement module is partly loaded onto in 1/4 space ((b) district among the figure) at original fluorescence imaging excitation portion place, just can be realized the dual module integral of PET and archebiosis light fault imaging.And, promptly can realize the dual module integral of PET and diffused light fault imaging as long as the bandpass filter in the original system is replaced by the type that allows exciting light pass through smoothly.
Claims (5)
1, the small animal molecule image-forming data-acquisition system of nucleic and fluorescent dual module integral, it is characterized in that, contain: cylinder imaging chamber, nucleic detect imaging subsystems, fluoroscopic examination imaging subsystems and computer, the lateral surface in this imaging chamber and in addition part two plane cuttings being orthogonal to described imaging chamber central axis be uniform four, described nucleic detects imaging subsystems and respectively accounts for two 1/4 relative measurement spaces with the fluoroscopic examination imaging subsystems, wherein:
Cylinder imaging chamber, contain: sidewall has the cavity main body of fluorescence excitation window respect to one another and fluoroscopic examination window, the support of the suspension toy that is positioned at intracavity and can again can in the vertical direction moves at the horizontal plane internal rotation, and link to each other with described support and can control support and horizontally rotate motor with vertical motion; This motor operates at following any rotation mode: revolve the switching spatial mode that turn 90 degrees or per step rotates a low-angle step one and adopts type or at the uniform velocity rotation, adopts type while changeing at every turn, so that make described nucleic detect imaging subsystems and the fluoroscopic examination imaging subsystems carries out the 360 panorama data acquisitions of spending to the toy whole body;
Nucleic detects imaging subsystems, contain: be evenly distributed on scintillation crystal bar and location-sensitive photomultiplier tube in outer two the 1/4 relative measurement space scopes of this imaging chamber lateral surface more than one, in order to detect the paired γ photon that nucleic sent that injects living small animal body internal labeling particular organization and cell; γ light is converted to visible light through scintillation crystal, is converted to the signal of telecommunication through photomultiplier tube again;
The fluoroscopic examination imaging subsystems, contain: laser instrument, photoswitch and excitation fiber are arranged in of two 1/4 relative spaces in addition beyond this imaging chamber lateral surface; Have at its 1/4 relative measurement space: receive optical fiber, protection stereotype group, dash receiver and optical filter, camera lens and charge coupled device ccd, wherein:
Excitation fiber and reception optical fiber, each is made of the multichannel multimode fibre, and exciting light enters described imaging chamber by excitation fiber from the fluorescence excitation window; Receive optical fiber and be close to described fluoroscopic examination window from surface, imaging chamber diverse location receiving optical signals, the fluorescence that this optical signal excites the back to be sent from the fluorescent probe stimulated luminescence that injects living small animal body internal labeling specific molecular or cell;
Photoswitch is a multi-channel optical fibre switch, and computer is realized the switching of multi-path laser source by the programme-control switching channel, and every road optical signal is coupled to this imaging chamber via excitation fiber;
Laser instrument is for selected photoswitch provides the fluorescence excitation light source;
Protection stereotype group, be positioned at described CCD camera lens before, area is avoided the high energy gamma photon damage CCD that overflows from toy tissue at least greater than the CCD visual field;
Computer, input signal are from through the output signal of telecommunication of photomultiplier tube with from the output signal of described CCD device, and output signal is rotation control and the switching signal that is controlled to picture intracavity motor; At this moment, also have, and calculate data image signal according to algorithm for reconstructing to the control signal of CCD camera with to the passage switch-over control signal of photoswitch.
2, the small animal imaging data-acquisition system of nucleic according to claim 1 and fluorescent dual module integral, it is characterized in that, the geometric ratio beam splitter of a single input dual output of the output termination of described laser instrument, one of them output arrives the imaging intracavity through reference optical fiber, be used to provide one and can eliminate the light intensity signal jitter error, realize the reference optical signal of measurement update, another output connects described photoswitch.
3, the small animal molecule image-forming data-acquisition system of nucleic according to claim 1 and 2 and fluorescent dual module integral is characterized in that, fills the optical match dielectric fluid at described cylinder imaging intracavity.
4, the small animal molecule image-forming data-acquisition system of nucleic according to claim 1 and 2 and fluorescent dual module integral is characterized in that, described cavity main body is made by the black matt material of watertight.
5, according to the small animal molecule image-forming data-acquisition system of claims 1 described nucleic and fluorescent dual module integral, it is characterized in that, in shared 1/4 space of fluorescence detection part, overflow the high energy gamma photon and damage the CCD that detects the low light level in order to prevent the border, use optical fiber is drawn the fluorescence signal on the discrete point of surface, imaging chamber, pass one group of protection stereotype again, to absorb the γ photon.
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| CN111781797B (en) * | 2020-07-16 | 2023-08-29 | 同济大学 | Multichannel bent crystal imaging system and assembling and adjusting method thereof |
| CN112345766B (en) * | 2020-10-22 | 2024-02-02 | 中国工程物理研究院核物理与化学研究所 | Fluorescence-radioactivity combined in-vitro targeting screening method |
| CN113109307A (en) * | 2021-04-01 | 2021-07-13 | 南京超维景生物科技有限公司 | Displacement control method and device, electronic equipment and multi-photon fluorescence imaging equipment |
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