CN106404641A - Micro particle counting device and method - Google Patents
Micro particle counting device and method Download PDFInfo
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- CN106404641A CN106404641A CN201610716420.9A CN201610716420A CN106404641A CN 106404641 A CN106404641 A CN 106404641A CN 201610716420 A CN201610716420 A CN 201610716420A CN 106404641 A CN106404641 A CN 106404641A
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- 239000011859 microparticle Substances 0.000 title claims abstract description 53
- 238000000034 method Methods 0.000 title claims abstract description 22
- 239000007788 liquid Substances 0.000 claims abstract description 167
- 239000002699 waste material Substances 0.000 claims abstract description 44
- 239000012530 fluid Substances 0.000 claims description 38
- 230000003287 optical effect Effects 0.000 claims description 10
- 230000005611 electricity Effects 0.000 claims description 6
- 229910052736 halogen Inorganic materials 0.000 claims description 3
- 150000002367 halogens Chemical class 0.000 claims description 3
- 238000013461 design Methods 0.000 abstract description 8
- 238000001514 detection method Methods 0.000 abstract description 2
- 230000008569 process Effects 0.000 description 5
- 238000010586 diagram Methods 0.000 description 3
- 238000009434 installation Methods 0.000 description 3
- 230000003068 static effect Effects 0.000 description 3
- 238000012545 processing Methods 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1434—Optical arrangements
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1434—Optical arrangements
- G01N2015/144—Imaging characterised by its optical setup
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N2015/1486—Counting the particles
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- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Physics & Mathematics (AREA)
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- Life Sciences & Earth Sciences (AREA)
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- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
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- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The present invention provides a micro particle counting device and method, and the micro particle counting device comprises a positive pressure control unit, a negative pressure control unit, a counting chip, a waste liquid collection container, an image acquisition unit, a processor and a liquid storage container with a sheath liquid stored in. The counting chip is provided with a sample adding hole and a liquid flow passage, the liquid flow passage is arranged in the counting chip, the liquid flow passage is communicated with the external part of the counting chip by the sample adding hole, both ends of the liquid flow passage are respectively connected with the liquid storage container and the waste liquid collection container, the positive pressure control unit is connected with the liquid storage container, the negative pressure control unit and the waste liquid collection container are connected, and the positive pressure control unit, the negative pressure control unit and the image acquisition unit are respectively connected with the processor electrically. Through the above design, the actual sample detection amount is increased, so that the calculated number of micro particles is more accurate.
Description
Technical field
The present invention relates to the counted fields of molecule, especially, it is related to a kind of microparticle counting device and method.
Background technology
At present, the method that the counting of cell or other molecules is typically based on image recognition is realized counting.Existing
Artificial counting and automated enumeration two ways are had based on the microparticle method of counting of image recognition.Wherein, artificial counting be by
Cell suspending liquid adds in the counting chamber of cell counting count board, with the naked eye examines under a microscope and carries out hand according to certain rule
Dynamic counting.Automated enumeration is then to add cell suspending liquid in the counting chamber of the count slice supporting with detecting instrument, will count
Piece inserting instrument detect tank, shoots static images by operating instrument to a certain region of counting chamber, then to described static images
Processed and counted.The tested sample size of reality of above-mentioned counting mode is relatively low, and the resultant error calculating is bigger than normal.
Content of the invention
In view of this, it is an object of the invention to provide a kind of microparticle counting device and method, to solve the above problems.
To achieve these goals, the embodiment of the present invention adopts the following technical scheme that：
A kind of microparticle counting device, holds including over-pressure control unit, vacuum cavitations unit, counting chip, waste collection
Device, image acquisition units, processor and the liquid storage container storing sheath fluid；
Described counting chip offers well and liquor stream runner, and described liquor stream runner is opened in the interior of described counting chip
Portion, described well by the ft connection of described liquor stream runner and described counting chip, the two ends of described liquor stream runner respectively with
Described liquid storage container is connected with waste liquid collection vessel, and described over-pressure control unit is connected with described liquid storage container, described negative pressure control
Unit processed is connected with described waste liquid collection vessel, and described over-pressure control unit, vacuum cavitations unit and image acquisition units are respectively
It is electrically connected with described processor；
Described processor is used for controlling described over-pressure control unit to produce malleation, controls described vacuum cavitations unit to produce negative
Air pressure so that the sheath fluid in described liquid storage container carry from described well add sample liquids through described liquor stream runner stream
To described waste liquid collection vessel, described image collecting unit is used for gathering the many of the described sample liquids flowing through described liquor stream runner
Open image, described processor is used for multiple images described are processed to obtain the microparticle number in described sample liquids.
Preferably, described liquor stream runner includes image acquisition areas, and described well is located at described liquor stream runner and connects institute
State between one end of liquid storage container and described image acquisition zone, described image collecting unit includes light source, camera and optical lens,
Described light source and camera are electrically connected with described processor respectively, and described camera passes through standard lens interface and described optical lens
Connect；
Described light source is arranged at the side of described counting chip, and towards described image acquisition zone, described camera is arranged at
The opposite side of described counting chip, and described optical lens is in the face of described image acquisition zone；
Described light source is used under the control of described processor to described image acquisition zone transmitted ray；Described camera is used for
Under the control of described processor, collection flows through multiple images of described sample liquids of described image acquisition zone and is sent to institute
State processor.
Preferably, described light source is LED/light source, monochromatic source, laser beam or Halogen lamp LED.
Preferably, described counting chip offers inlet opening and outage, and described inlet opening and outage are located at institute respectively
State the two ends of liquor stream runner, and with described liquor stream flow passage；
Described liquid storage container is connected with described liquor stream runner by described inlet opening, and described waste liquid collection vessel passes through described
Outage is connected with described liquor stream runner.
Preferably, it is provided with the first magnetic valve between described over-pressure control unit and described liquid storage container, described liquid storage holds
Second magnetic valve is set between device and described inlet opening, between described outage and described waste liquid collection vessel, is provided with the 3rd electricity
Magnet valve, is provided with the 4th magnetic valve between described waste liquid collection vessel and described vacuum cavitations unit；
Described first magnetic valve and the second magnetic valve are electrically connected with described over-pressure control unit, described 3rd magnetic valve and
4th magnetic valve is electrically connected with described vacuum cavitations unit.
Preferably, described first magnetic valve and described 4th magnetic valve are two-bit triplet magnetic valve, described second magnetic valve
It is 2/2-way magnetic valve with described 3rd magnetic valve.
Preferably, it is provided with the first check valve, described outage and institute between described second magnetic valve and described inlet opening
State and be provided with the second check valve between the 3rd magnetic valve；
Described first check valve and described over-pressure control unit electric connection, described second check valve and described vacuum cavitations
Unit is electrically connected with.
Preferably, described over-pressure control unit includes malleation drive component, and described malleation drive component is held with described liquid storage
Device is connected, and described first magnetic valve is arranged between described malleation drive component and described liquid storage container；
Described vacuum cavitations unit includes negative pressure drive component, and described negative pressure drive component and described waste liquid collection vessel are even
Logical, described 4th magnetic valve is arranged between described negative pressure drive component and described waste liquid collection vessel；
Described malleation drive component is used for for the sheath fluid in described liquid storage container pushing described liquor stream runner, and described negative pressure is driven
Dynamic assembly is used for for the sheath fluid in described liquor stream runner and sample liquids sucking described waste liquid collection vessel.
Preferably, described over-pressure control unit also includes positive pressure pressure controller, and described vacuum cavitations unit also includes bearing
Pressure pressure controller, described positive pressure pressure controller and negative pressure pressure controller are electrically connected with described processor respectively；
Described positive pressure pressure controller is connected with described malleation drive component, the first magnetic valve and the second magnetic valve, described
Negative pressure pressure controller is electrically connected with described negative pressure drive component, the 3rd magnetic valve and the 4th magnetic valve.
Another embodiment of the present invention also provides a kind of microparticle technology of the microparticle counting device based on offer of the present invention
Method, methods described includes：
Start described over-pressure control unit, and detect the pressure value that described over-pressure control unit produces；
When the pressure value producing in described over-pressure control unit reaches the first preset pressure threshold value, control described over-pressure control
Unit applies a malleation to described liquid storage container, so that the sheath fluid in described liquid storage container flows into described liquor stream runner；
When described liquor stream runner is filled up by the sheath fluid in described liquid storage container, sample liquid is added by described well
Body；
Start described vacuum cavitations unit, and detect the pressure value that described vacuum cavitations unit produces；
When the pressure value producing in described vacuum cavitations unit reaches the second preset pressure threshold value, control described vacuum cavitations
Unit produces a negative pressure to described waste liquid collection vessel, so that described sheath fluid carries described sample liquids through described liquor stream stream
Road flows to described waste liquid collection vessel；
The collection of described image collecting unit is controlled to flow through multiple images of described sample liquids of described liquor stream runner right
Multiple images described are processed to obtain the microparticle number in described sample liquids.
Microparticle counting device provided in an embodiment of the present invention and method utilize over-pressure control unit and vacuum cavitations unit
Order about sheath fluid in liquid storage container carry from well add sample liquids flow to waste liquid collection vessel through liquor stream runner, utilize
Image acquisition units collection flows through multiple images of the described sample liquids of described liquor stream runner, and using processor to described many
Open image to carry out processing the microparticle number obtaining in described sample liquids.By above-mentioned design, increased in detection process
Actual sample quantity thus realize particle under flow regime high flux count so that the microparticle number finally giving more
For accurate, the uniformity of measurement result is more preferable.
Brief description
In order to be illustrated more clearly that the technical scheme of the embodiment of the present invention, below will be attached to use required in embodiment
Figure is briefly described it will be appreciated that the following drawings illustrate only certain embodiments of the present invention, and it is right to be therefore not construed as
The restriction of scope, for those of ordinary skill in the art, on the premise of not paying creative work, can also be according to this
A little accompanying drawings obtain other related accompanying drawings.
Fig. 1 is a kind of connection block diagram of microparticle counting device provided in an embodiment of the present invention.
Fig. 2 is a kind of structural representation of counting chip provided in an embodiment of the present invention.
Fig. 3 is the schematic view of the mounting position of light source provided in an embodiment of the present invention and camera.
Fig. 4 is the electric connecting relation signal of malleation drive component provided in an embodiment of the present invention and negative pressure drive component
Figure.
Fig. 5 is the electric connecting relation schematic diagram of each magnetic valve provided in an embodiment of the present invention.
Fig. 6 is the electric connecting relation schematic diagram of each part of microparticle counting device provided in an embodiment of the present invention.
Fig. 7 is the schematic view of the mounting position of each part of microparticle counting device provided in an embodiment of the present invention.
Fig. 8 is a kind of schematic view of the mounting position of over-pressure control unit provided in an embodiment of the present invention.
Fig. 9 is another schematic view of the mounting position of over-pressure control unit provided in an embodiment of the present invention.
Figure 10 is a kind of schematic flow sheet of microparticle method of counting provided in an embodiment of the present invention.
Reference：
100- microparticle counting device；
110- over-pressure control unit, 111- malleation drive component, 112- positive pressure pressure controller；
120- vacuum cavitations unit, 121- negative pressure drive component, 122- negative pressure pressure controller；
130- liquid storage container；
140- counting chip, 141- inlet opening, 142- outage, 143- well, 144- image acquisition areas, 145- liquor stream
Runner；
150- waste liquid collection vessel；
160- image acquisition units, 161- light source, 162- camera；
170- processor；
181- first magnetic valve, 182- second magnetic valve, 183- the 3rd magnetic valve, 184- the 4th magnetic valve, 185- first
Check valve, 186- second check valve.
Specific embodiment
Purpose, technical scheme and advantage for making the embodiment of the present invention are clearer, below in conjunction with the embodiment of the present invention
In accompanying drawing, the technical scheme in the embodiment of the present invention is clearly and completely described it is clear that described embodiment is
The a part of embodiment of the present invention, rather than whole embodiments.The present invention generally described and illustrated in accompanying drawing herein is implemented
The assembly of example can be arranged with various different configurations and design.
Therefore, below the detailed description of the embodiments of the invention providing in the accompanying drawings is not intended to limit claimed
The scope of the present invention, but be merely representative of the selected embodiment of the present invention.Based on the embodiment in the present invention, this area is common
The every other embodiment that technical staff is obtained under the premise of not making creative work, broadly falls into the model of present invention protection
Enclose.
It should be noted that：Similar label and letter represent similar terms in following accompanying drawing, therefore, once a certain Xiang Yi
It is defined in individual accompanying drawing, then do not need it to be defined further and explains in subsequent accompanying drawing.
As shown in figure 1, embodiments providing a kind of microparticle counting device 100.Described microparticle counting device
100 are used for shooting multiple images of sample liquids, and multiple images described are processed, to obtain in described sample liquids
Microparticle number.It should be noted that the microparticle referring in the embodiment of the present invention includes zooblast, yeast, fungal spore
Deng microparticle, but not limited to this.
Refer to Fig. 1, described microparticle device includes over-pressure control unit 110, vacuum cavitations unit 120, counting chip
140th, waste liquid collection vessel 150, image acquisition units 160, processor 170 and the liquid storage container 130 storing sheath fluid.Specifically
Ground, the two ends of described counting chip 140 are connected with described liquid storage container 130 and waste liquid collection vessel 150 respectively, described just voltage-controlled
Unit 110 processed is connected with described liquid storage container 130, and described vacuum cavitations unit 120 is connected with described waste liquid collection vessel 150,
Described over-pressure control unit 110, vacuum cavitations unit 120 are connected with described processor 170 respectively with image acquisition units 160.
Referring again to Fig. 2, it is a kind of structural representation of counting chip 140 provided in an embodiment of the present invention.Described counting
Chip 140 offers well 143 and liquor stream runner 145, and described liquor stream runner 145 is opened in the interior of described counting chip 140
Portion, described well 143 by the ft connection of described liquor stream runner 145 and described counting chip 140 so that sample liquids can
It is added in described liquor stream runner 145 from described well 143.
Alternatively, described counting chip 140 can offer inlet opening 141 and outage 142, described inlet opening 141 He
Outage 142 is located at the two ends of described liquor stream runner 145 respectively, and connects with described liquor stream runner 145.Above-mentioned liquid storage container
130 are connected with described liquor stream runner 145 by described inlet opening 141, and above-mentioned waste liquid collection vessel 150 passes through described outage
142 are connected with described liquor stream runner 145.
Alternatively, described liquor stream runner 145 also includes image acquisition areas 144, and described well 143 is located at described liquor stream
One end that runner 145 connects described liquid storage container 130 is between described inlet opening 141 and described image acquisition zone 144, so that institute
State the sheath fluid in liquid storage container 130 fully to merge with the sample liquids adding from described well 143, to avoid due to sample
Product liquid and the uneven measure error causing of suspension of sheath fluid composition.
During enforcement, start described over-pressure control unit 110, so that described over-pressure control unit 110 is to described liquid storage container
130 generation one malleations, the sheath fluid of storage in liquid storage container 130 is pushed the liquor stream runner 145 within counting chip 140.Work as institute
When stating sheath fluid entrance liquor stream runner 145 and described liquor stream runner being filled up, add sample liquids from described well 143, and open
Move described vacuum cavitations unit 120, so that described vacuum cavitations unit 120 produces one to described waste liquid collection vessel 150 doing something in a fit of pique
Pressure, the sheath fluid in liquor stream runner 145 and sample liquids are sucked described waste liquid collection vessel 150 in the lump.
Above-mentioned design makes the sheath fluid in liquid storage container 130 carry the sample liquids process added from described well 143
Described liquor stream runner 145 flows to waste liquid collection vessel 150.Now, using described image collecting unit 160 to flowing through described liquor stream
The described sample liquids of runner 145 are taken pictures, and to obtain multiple images of described sample liquids, and multiple images getting are sent out
Deliver to described processor 170 to be processed, you can obtain the microparticle number in described sample liquids.
As shown in figure 3, alternatively, described image collecting unit 160 can include light source 161, camera 162 and optical lens
163, described light source 161 and camera 162 are electrically connected with described processor 170 respectively, and described optical lens 163 passes through a standard
Lens interface is connected with described camera 162.Wherein, described optical lens 163 can be single lens or the group of multiple camera lens
Close.Wherein, described light source 161 is arranged at the side of described counting chip 140, and towards above-mentioned image acquisition areas 144, described phase
Machine 162 is arranged at the opposite side of described counting chip 140, and described optical lens 163 is in the face of above-mentioned image acquisition areas 144.Logical
Cross above-mentioned design so that described light source 161 can be to above-mentioned image acquisition areas 144 transmission under the control of described processor 170
Light, described camera 162 can gather the described sample flowing through above-mentioned image acquisition areas 144 under the control of described processor 170
Multiple images of product liquid, and be sent to described processor 170 and processed.
Alternatively, described light source 161 can be LED/light source, monochromatic source, laser beam or Halogen lamp LED, but is not limited to this.
As shown in figure 4, described over-pressure control unit 110 can include malleation drive component 111, described vacuum cavitations unit
120 can include negative pressure drive component 121, and described malleation drive component 111 is used for pushing away the sheath fluid in described liquid storage container 130
Enter described liquor stream runner 145, described negative pressure drive component 121 is used for the sheath fluid in described liquor stream runner 145 and sample liquids
Suck described waste liquid collection vessel 150.Described malleation drive component 111 and negative pressure drive component 121 respectively with described processor
170 electric connections so that described processor 170 control the unlatching of described malleation drive component 111 and negative pressure drive component 121 with
Close.
According to the actual requirements, alternatively, described malleation drive component 111 can be positive pressure gas pump or liquid pump, when described
When malleation drive component 111 is malleation air pump, described malleation air pump can be diaphragm air pump.When described malleation drive component 111
During for liquid pump, Ke Yiyou, but be not limited to, following selection：Peristaltic pump, membrane pump and linear pump.Described negative pressure drive component 121
For negative pressure air pump.
Alternatively, as shown in figure 5, described microparticle counting device 100 can also include first magnetic valve the 181, second electricity
Magnet valve 182, the 3rd magnetic valve 183 and the 4th magnetic valve 184.
According to the actual requirements, a described magnetic valve 181, the second magnetic valve 182, the 3rd magnetic valve 183 and the 4th magnetic valve
184 can be electrically connected with described processor 170 respectively, so that processor 170 directly controls a described magnetic valve 181, second
The open and close of magnetic valve 182, the 3rd magnetic valve 183 and the 4th magnetic valve 184.
Alternatively, as shown in fig. 6, described over-pressure control unit 110 can also include and described malleation drive component 111 electricity
Property the positive pressure pressure controller 112 that connects, described vacuum cavitations unit 120 can also include and described negative pressure drive component 121
The negative pressure pressure controller 122 being electrically connected with.Described first magnetic valve 181 and the second magnetic valve 182 respectively with positive pressure pressure control
Device 122 processed is electrically connected with, so that processor 170 can control described first magnetic valve by described positive pressure pressure controller 112
181 and second magnetic valve 182.Described 3rd magnetic valve 183 and the 4th magnetic valve 184 respectively with described negative pressure pressure controller
122 electric connections, so that described processor 170 can control described 3rd magnetic valve 183 He by negative pressure pressure controller 122
4th magnetic valve 184.
See also Fig. 7, it is unidirectional that described microparticle counting device 100 can also include the first check valve 185 and second
Valve 186.Fig. 7 is described first magnetic valve 181, the second magnetic valve 182, the 3rd magnetic valve 183, the 4th magnetic valve the 184, first list
Schematic view of the mounting position to valve 185 and the second check valve 186.
Alternatively, described first magnetic valve 181 is connected to described liquid storage container 130 away from the one of above-mentioned counting chip 140
End.Described second magnetic valve 182 is arranged between described liquid storage container 130 and inlet opening 141, for controlling liquid storage container 130
The break-make of the fluid path and inlet opening 141 between.Described 3rd magnetic valve 183 is arranged at outage 142 and waste liquid collection vessel 150
Between, for controlling the break-make of the fluid path between outage 142 and waste liquid collection vessel 150.Described 4th magnetic valve 184 is arranged
Between waste liquid collection vessel 150 and vacuum cavitations unit 120.Described first check valve 185 is arranged at described second magnetic valve
Between 182 and described inlet opening 141, for avoiding the liquid in liquor stream runner 145 to be back in described liquid storage container 130.Institute
State the second check valve 186 to be arranged between described outage 142 and described 3rd magnetic valve 183, be used for avoiding waste collection to hold
Liquid in device 150 flows into described liquor stream runner 145.
Wherein, the concrete installation site of described over-pressure control unit 110 and the type of described malleation drive component 111 have
Close.As shown in figure 8, when described malleation drive component 111 is liquid pump, described over-pressure control unit 110 is arranged at described liquid storage
Between container 130 and the second magnetic valve 182.
As shown in figure 9, when described malleation drive component 111 is malleation air pump, described over-pressure control unit 110 is connected to
Described liquid storage container 130 is away from one end of described counting chip 140, so that described first magnetic valve is arranged at described over-pressure control
Between unit 110 and liquid storage container 130.During enforcement, described first magnetic valve 181 is used for controlling over-pressure control unit 110 and storage
The break-make of the gas circuit between liquid container 130.
Alternatively, described first magnetic valve 181 and the 4th magnetic valve 184 are two-bit triplet magnetic valve, and two of which connects
, for controlling the break-make of gas circuit, another connector is laggard for closing in described first magnetic valve 181 and the 4th magnetic valve 184 for mouth
Row exhaust.Described second magnetic valve 182 and the 3rd magnetic valve 183 are 2/2-way magnetic valve, for controlling the break-make of fluid path.
It should be appreciated that Fig. 4 and Fig. 6 is only the electricity of each part of microparticle counting device 100 provided in an embodiment of the present invention
Gas annexation figure, the installation site of each part does not show that, the concrete installation site of each part refers to Fig. 5, Fig. 7 and Fig. 9.
During enforcement, after described over-pressure control unit 110 starts, described malleation drive component 111 produces a malleation, works as institute
When stating the value of malleation and reaching the first preset pressure threshold value, described processor 170 assigns a control to described positive pressure pressure controller 112
System instruction, so that described positive pressure pressure controller 112 controls described first magnetic valve 181 and the second magnetic valve 182 to open.Described
After first magnetic valve 181, the second magnetic valve 182 are opened, the sheath fluid in liquid storage container 130 is from described liquor stream runner 145 near institute
The port stating the second magnetic valve 182 is pushed out, and when the pressure of loop inner reaches certain value, described first check valve 185 is opened
Open, then the sheath fluid in liquid storage container 130 is pressed into described liquor stream runner 145.Meanwhile, described in described processor 170 controls
Light source 161 is opened, so that described light source 161 is towards described image acquisition zone 144 transmitted ray.
After the sheath fluid in described liquid storage container 130 is pressed into described liquor stream runner 145, open described vacuum cavitations unit
120.Described negative pressure drive component 121 produces a negative pressure, when the value of described negative pressure reaches the second preset pressure threshold value, institute
State processor 170 and assign a control instruction to described negative pressure pressure controller 122, so that described negative pressure pressure controller 122 is controlled
Make described 3rd magnetic valve 183 and the 4th magnetic valve 184 is opened.Described 3rd magnetic valve 183 and the 4th magnetic valve 184 are opened
Afterwards, the sheath fluid in liquor stream runner 145 and sample liquids are inhaled near one end of the second check valve 186 from described liquor stream runner 145
Go out, when loop internal pressure reaches certain value, described second check valve 186 automatically turns on, then the sheath fluid in liquor stream runner 145 and
Sample liquids are inhaled into described waste liquid collection vessel 150.
Above-mentioned design makes the sheath fluid in liquid storage container 130 and the sample liquids from well 143 interpolation in liquor stream runner
Flow in 145, shoot multiple images of the sample liquids flowing through described liquor stream runner 145 by described camera 162, and right
Multiple images described carry out processing the microparticle number that can get in described sample liquids.
It should be noted that the Opening pressure of described first check valve 185 and the second check valve 186 and selected check valve
Self performance is relevant.Multiple images described are to be continuously shot, that is, within the reaction time of described processor 170 and camera 162, to the greatest extent
Soon the sample liquids in described liquor stream runner 145 may be continuously shot, thus further increasing actual detected sample
This amount, reduces sample error.
In the present embodiment, what deserves to be explained is, the process of multiple images to described sample liquids for the described processor 170
Can be direct process, image is processed using being stored in embedded software in described processor 170.Can also be logical
Cross external equipment indirectly to process, for example, described plurality of pictures is sent to host computer, carries out image spelling using image processing software
Connect, to obtain an image possessing the whole sampled points in multiple images described.The method recycling feature extraction, calculates
Microparticle number in described image.It should be appreciated that image processing method described herein is only for example, it is not intended as limiting.
The embodiment of the present invention also provides a kind of microparticle counting side of the microparticle counting device 100 based on offer of the present invention
Method, as shown in Figure 10, described microparticle method of counting comprises the following steps：
S201：Start described over-pressure control unit 110, and detect the pressure value that described over-pressure control unit 110 produces.
S202：When the pressure value producing in described over-pressure control unit 110 reaches the first preset pressure threshold value, control described
Over-pressure control unit 110 applies a malleation to described liquid storage container 130, so that the sheath fluid in described liquid storage container 130 flows into institute
State liquor stream runner 145.
S203：When described liquor stream runner 145 is filled up by the sheath fluid in described liquid storage container 130, by described well
143 interpolation sample liquids.
S204：Start described vacuum cavitations unit 120, and detect the pressure value that described vacuum cavitations unit 120 produces.
S205：When the pressure value producing in described vacuum cavitations unit 120 reaches the second preset pressure threshold value, control described
Vacuum cavitations unit 120 produces a negative pressure to described waste liquid collection vessel 150, so that described sheath fluid carries described sample liquids
Flow to described waste liquid collection vessel 150 through described liquor stream runner 145.
S206：Described image collecting unit 160 collection is controlled to flow through the many of the described sample liquids of described liquor stream runner 145
Open image and multiple images described are processed to obtain the microparticle number in described sample liquids.
By above-mentioned design, the sheath fluid in liquid storage container 130 is pushed into liquor stream runner 145 and by described liquor stream runner
145 when filling up, and adds sample liquids by well 143, it can be avoided that described sample liquids are in the drying section of liquor stream runner 145
Position spreads out, and leads to not detect.
In sum, microparticle counting device provided in an embodiment of the present invention and method, by over-pressure control unit 110 He
Vacuum cavitations unit 120 drive liquid storage container 130 in sheath fluid carry from well 143 add sample liquids through described liquid
Stream runner 145 flows to described waste liquid collection vessel 150, using image acquisition units 160 to flowing through described image acquisition zone 144
Sample liquids shoot plurality of pictures, and by processor 170, described plurality of pictures are processed, you can obtain described sample liquid
Microparticle number in body.Above-mentioned design increased actual tested sample size, thus keep away reducing measure error.Additionally,
Sample liquids in flowing are shot with multiple images also avoid when only shooting static images due to microparticle suspension not
The measure error uniformly causing.
In describing the invention, it should be noted that term " on ", D score, " interior ", the orientation of instruction or the position such as " outward "
The relation of putting is based on orientation shown in the drawings or position relationship, or the orientation usually put or position when this invention product uses
Put relation, be for only for ease of and describe the present invention and simplify description, rather than indicate or imply that the device of indication or element are necessary
There is specific orientation, with specific azimuth configuration and operation, be therefore not considered as limiting the invention.
In describing the invention in addition it is also necessary to explanation, should be interpreted broadly term " setting " " being connected ", " connection ",
For example, it may be being fixedly connected or being detachably connected, or it is integrally connected；Can be to be mechanically connected or electricity
Connect；Can be to be joined directly together it is also possible to be indirectly connected to by intermediary, can be the connection of two element internals.For
For those of ordinary skill in the art, above-mentioned term concrete meaning in the present invention can be understood with concrete condition.
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, for the skill of this area
For art personnel, the present invention can have various modifications and variations.All within the spirit and principles in the present invention, made any repair
Change, equivalent, improvement etc., should be included within the scope of the present invention.
Claims (10)
1. a kind of microparticle counting device is it is characterised in that including over-pressure control unit, vacuum cavitations unit, counting chip, giving up
Liquid collecting container, image acquisition units, processor and the liquid storage container storing sheath fluid；
Described counting chip offers well and liquor stream runner, and described liquor stream runner is opened in the inside of described counting chip,
Described well by the ft connection of described liquor stream runner and described counting chip, the two ends of described liquor stream runner respectively with described
Liquid storage container is connected with waste liquid collection vessel, and described over-pressure control unit is connected with described liquid storage container, described vacuum cavitations list
First and described waste liquid collection vessel is connected, described over-pressure control unit, vacuum cavitations unit and image acquisition units respectively with institute
State processor to be electrically connected with；
Described processor is used for controlling described over-pressure control unit to produce malleation, controls described vacuum cavitations unit to produce and does something in a fit of pique
Pressure, so that the sheath fluid in described liquid storage container carries the sample liquids adding from described well and flows to through described liquor stream runner
Described waste liquid collection vessel, described image collecting unit is used for gathering multiple of the described sample liquids flowing through described liquor stream runner
Image, described processor is used for multiple images described are processed to obtain the microparticle number in described sample liquids.
2. microparticle counting device according to claim 1 is it is characterised in that described liquor stream runner includes an IMAQ
Area, described well is located between one end and the described image acquisition zone that described liquor stream runner connects described liquid storage container, described
Image acquisition units include light source, camera and optical lens, and described light source and camera are electrically connected with described processor respectively, institute
State camera to be connected with described optical lens by standard lens interface；
Described light source is arranged at the side of described counting chip, and towards described image acquisition zone, described camera is arranged at described
The opposite side of counting chip, and described optical lens is in the face of described image acquisition zone；
Described light source is used under the control of described processor to described image acquisition zone transmitted ray；Described camera is used in institute
The lower collection that controls stating processor flows through multiple images of described sample liquids of described image acquisition zone and is sent to described place
Reason device.
3. microparticle counting device according to claim 2 is it is characterised in that described light source is LED/light source, monochromatic light
Source, laser beam or Halogen lamp LED.
4. microparticle counting device according to claim 1 it is characterised in that described counting chip offer inlet opening and
Outage, described inlet opening and outage respectively be located at described liquor stream runner two ends, and with described liquor stream flow passage；
Described liquid storage container is connected with described liquor stream runner by described inlet opening, and described waste liquid collection vessel is by described discharge opeing
Hole is connected with described liquor stream runner.
5. microparticle counting device according to claim 4 is it is characterised in that described over-pressure control unit and described liquid storage
It is provided with the first magnetic valve between container, the second magnetic valve, described discharge opeing are set between described liquid storage container and described inlet opening
Be provided with the 3rd magnetic valve between hole and described waste liquid collection vessel, described waste liquid collection vessel and described vacuum cavitations unit it
Between be provided with the 4th magnetic valve；
Described first magnetic valve and the second magnetic valve are electrically connected with described over-pressure control unit, described 3rd magnetic valve and the 4th
Magnetic valve is electrically connected with described vacuum cavitations unit.
6. microparticle counting device according to claim 5 it is characterised in that described first magnetic valve and described 4th electricity
Magnet valve is two-bit triplet magnetic valve, and described second magnetic valve and described 3rd magnetic valve are 2/2-way magnetic valve.
7. microparticle counting device according to claim 6 is it is characterised in that described second magnetic valve and described inlet opening
Between be provided with the first check valve, be provided with the second check valve between described outage and described 3rd magnetic valve；
Described first check valve and described over-pressure control unit electric connection, described second check valve and described vacuum cavitations unit
It is electrically connected with.
8. microparticle counting device according to claim 7 is it is characterised in that described over-pressure control unit includes malleation drive
Dynamic assembly, described malleation drive component is connected with described liquid storage container, and described first magnetic valve is arranged at described malleation driving group
Between part and described liquid storage container；
Described vacuum cavitations unit includes negative pressure drive component, and described negative pressure drive component connects with described waste liquid collection vessel,
Described 4th magnetic valve is arranged between described negative pressure drive component and described waste liquid collection vessel；
Described malleation drive component is used for for the sheath fluid in described liquid storage container pushing described liquor stream runner, described negative pressure driving group
Part is used for for the sheath fluid in described liquor stream runner and sample liquids sucking described waste liquid collection vessel.
9. microparticle counting device according to claim 8 is it is characterised in that described over-pressure control unit also includes malleation
Pressure controller, described vacuum cavitations unit also includes negative pressure pressure controller, described positive pressure pressure controller and negative pressure pressure
Controller is electrically connected with described processor respectively；
Described positive pressure pressure controller is connected with described malleation drive component, the first magnetic valve and the second magnetic valve, described negative pressure
Pressure controller is electrically connected with described negative pressure drive component, the 3rd magnetic valve and the 4th magnetic valve.
10. the microparticle method of counting of the microparticle counting device described in a kind of any one based on claim 1-9, its feature
It is, methods described includes：
Start described over-pressure control unit, and detect the pressure value that described over-pressure control unit produces；
When the pressure value producing in described over-pressure control unit reaches the first preset pressure threshold value, control described over-pressure control unit
Apply a malleation to described liquid storage container, so that the sheath fluid in described liquid storage container flows into described liquor stream runner；
When described liquor stream runner is filled up by the sheath fluid in described liquid storage container, sample liquids are added by described well；
Start described vacuum cavitations unit, and detect the pressure value that described vacuum cavitations unit produces；
When the pressure value producing in described vacuum cavitations unit reaches the second preset pressure threshold value, control described vacuum cavitations unit
Produce a negative pressure to described waste liquid collection vessel, so that described sheath fluid carries described sample liquids through described liquor stream runner stream
To described waste liquid collection vessel；
The collection of described image collecting unit is controlled to flow through multiple images of described sample liquids of described liquor stream runner and to described
Multiple images are processed to obtain the microparticle number in described sample liquids.
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