CN101435818A - Portable Mediterranean anemia disease screening apparatus based on micro-flow control chip image technology - Google Patents
Portable Mediterranean anemia disease screening apparatus based on micro-flow control chip image technology Download PDFInfo
- Publication number
- CN101435818A CN101435818A CN 200810218356 CN200810218356A CN101435818A CN 101435818 A CN101435818 A CN 101435818A CN 200810218356 CN200810218356 CN 200810218356 CN 200810218356 A CN200810218356 A CN 200810218356A CN 101435818 A CN101435818 A CN 101435818A
- Authority
- CN
- China
- Prior art keywords
- microchannel
- micro
- fluidic chip
- screening apparatus
- substrate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The invention relates to a portable type thalassemia screening device based on microfluidic chip imaging technology. The device comprises a microfluidic chip, a microinjection pump, an optical detection device, a digital signal processor and a display screen, wherein the microfluidic chip is provided with at least two inlets and at least one outlet; the inside of the microfluidic chip is provided with a microchannel; the microchannel comprises a liquid mixing section and a detection section; the input end of the liquid mixing section is communicated with the inlets; the detection section is communicated with the outlet; the microinjection pump is communicated with the inlets of the microfluidic chip; the optical detection device is fixed on the upper side and the lower side of the detection section of the microchannel of the microfluidic chip; and the digital signal processor is connected with an image sensor of the optical detection device. The portable type thalassemia screening device integrates the solution preparation and the detecting operation on the chip through once completion, has small size and is convenient to carry and easy to operate.
Description
Technical field
The present invention relates to portable thalassemia screening apparatus, particularly a kind of portable thalassemia screening apparatus based on the micro-fluid chip image technology.
Background technology
Thalassemia (Thalassemias) is under the jurisdiction of hemoglobinopathy, but does not relate to the unusual of hemoglobin construct, and it is the heredity blood disease that causes owing to certain class globin dyssynthesis.The main laboratory inspection method of thalassemia comprises routine blood test mensuration, red blood cell morphology inspection, the inspection of denatured hemoglobin inclusion body, isopropanol test, the white cellulose acetate membrane electrophoresis of Trace Blood red eggs, erythrocyte osmotic fragility test (a pipe method).In thalassemic multiple inspection method, erythrocyte osmotic fragility test (a pipe method) is a kind of short-cut method of thalassemia population screening, simple to operate, quick, also have higher susceptibility and accuracy, cooperate mean corpuscular volume (MCV) and the average haemoglobin of red blood cell indexs such as (MCH), can carry out the thalassemia examination.
Micro-fluidic chip is a Biochemical Lab that makes up on more than one square centimeters chip, it is based on MEMS (Micro Electro Mechanical systems) processing technology, at silicon chip, make microchannel on the material such as glass or PDMS, and form network by the microchannel, run through total system with controlled fluid, realize related reaction in the biological and chemical field, separate, check, basic operations such as cellular incubation, in order to replace the breadboard various functions of conventional biological or chemical, therefore be also referred to as " laboratory on the chip (Lab-on-a-chip) ".Have characteristics such as tangible microminiaturization, integrated and portability.
It is a kind of widely used universal optical detection method that the absorbance that uses on micro-fluidic chip detects, and it has advantages such as the substance classes that can measure is many, apparatus structure is simpler.The molecule that this detection method is based on measured matter has the characteristic that selectivity absorbs and the analytical approach of setting up to light.Measure lower limit and can reach 10
-5～10
-6Mol/L is equivalent to content 10
-4%～10
-5The microcomponent of %.Absorption spectrophotometry is exactly a material to the degree of absorption of light for an important evidence of the quantitative and qualitative analysis of material is absorbance.When a branch of parallel monochromatic light shone the solution of homogeneous substance, the part of light was absorbed, and another part sees through solution, was absorbance (light longitude D or dullness E) to the degree of absorption of light.Lambert's beer's law is the basis of absorbance quantitative analysis.
Summary of the invention
The purpose of this invention is to provide a kind of small-sized, portable, thalassemia screening apparatus cheaply, this screening apparatus is by gathering the checked object small amounts of blood, inject micro-fluidic chip with certain density NaCl salt solusion, mix the back and detect the basic characteristic parameter of red blood cell in the blood, can reflect the health status of checked object with optical means.
The present invention is based on the portable thalassemia screening apparatus of micro-fluidic chip image technology, comprising:
Micro-fluidic chip has at least two inlets and at least one outlet, and the microchannel is set in it, and described microchannel comprises liquid mixing section and detection segment, and the input end of liquid mixing section is communicated with described inlet, and detection segment is communicated with described outlet;
Micro-injection pump is communicated with the inlet of described micro-fluidic chip, is used for blood to be measured and salt solusion are pumped into microchannel in the micro-fluidic chip;
Optical detection device comprises light source and imageing sensor, and they are individually fixed in the both sides up and down of the microchannel detection segment of described micro-fluidic chip, and light source, microchannel detection segment and imageing sensor are positioned on the same optical axis;
Digital signal processor is connected with described imageing sensor, and detected signal is carried out the extraction of gray-scale value and cell edges, needed absorbance value of the luminosity method of inspection that is absorbed and the required cell characteristic value of diagnosis; With,
Display screen is connected with described digital signal processor, is used to show check result.
The liquid mixing section of described microchannel can be the serpentine structure of S shape or shapes such as Z-shaped or bow font, and its internal diameter is 5～200 microns.The detection segment of described microchannel can be designed to xsect and be circle, triangle, semicircle or polygonal liquid storage tank, and the degree of depth of liquid storage tank can be designed to 20～100 microns, preferred 30～60 microns of the degree of depth.
Above-mentioned micro-fluidic chip is made by the substrate involution of two printing opacities, form the microstructure that constitutes described microchannel on one of them substrate, another substrate is close to opening one side of described microstructure, behind two substrate involutions the open side of described microstructure is sealed formation described microchannel (comprising liquid mixing section and detection segment), wherein, substrate can adopt PDMS, glass or other macromolecular material to make.
Described light source is a led light source, and described imageing sensor adopts cmos image sensor, and pixel size can reach 2 μ m.
The major function of screening apparatus of the present invention comprises the measurement of erythrocyte fragility check and red blood cell basic parameter.After blood sample enters micro-fluidic chip,, obtain several important hematological indices such as erythrocytic approx. dimension, comprise erythrocytic form, size, average external volume (MCV), cell count etc. by the CMOS direct imaging.Liquid mixing section in the microchannel, blood and salt solution mix, in 0.32% or 0.36% NaCl solution, normal person's red blood cell can be expanded to and break, haemoglobin is discharged in the solution, solution is clarified, and the poor patient's in ground red blood cell can not break, solution is muddy, blood-salt water mixed liquid flows into the liquid storage tank (being the detection segment of microchannel) in the chip, LED, liquid storage tank and cmos image sensor in the chip are on the same optical axis, use LED visible emitting irradiation micro-fluidic chip, blood in the microchannel-salt water mixed liquid projection on cmos image sensor, is judged that by the projection gray level situation of calculating on the CMOS blood is from normal person or the poor patient in ground.
This portable thalassemia screening apparatus adopts micro-fluidic chip technology pair cell to operate and analyze, and compares with present breadboard common method, and have following main advantage: 1) microchannel size and cell size are complementary; 2) micro-fluidic chip provides the space of a relative closure, has reduced the stimulation of external environment pair cell; 3) the micro-fluidic chip volume is small, has saved the consumption of cell solution and other reagent, and the microchannel heat and mass is rapid, and the reaction time also significantly reduces; 4) solution preparation and detecting operation are integrated in the chip piece last time and finish.
It uses high-resolution cmos imaging device to the micro-fluidic chip direct imaging, image processing method such as combining image feature extraction on the basis that absorbance detects to the Mediterranean anemia disease screening, makes that device can miniaturization, be easy to carry, and easy operating.
Description of drawings
Fig. 1 is the schematic diagram of this portable thalassemia screening apparatus;
Fig. 2 is the structural representation of the microchannel of its micro-fluidic chip;
Fig. 3 is the external structure synoptic diagram of its micro-fluidic chip.
Embodiment
The present invention will be further described below in conjunction with accompanying drawing.
With reference to Fig. 1-3, this comprises based on the portable thalassemia screening apparatus of micro-fluidic chip image technology:
Micro-fluidic chip 1, have 11,12 and outlets 13 of two inlets, the microchannel is set in it, described microchannel comprises liquid mixing section 14 and detection segment 15, the input end of liquid mixing section 14 is communicated with described two inlets 11,12, and detection segment 15 is communicated with described outlet 13;
Micro-injection pump 2 is communicated with two of described micro-fluidic chip inlets 11,12, is used for blood to be measured and salt solusion are pumped into the interior microchannel of micro-fluidic chip 1;
Optical detection device 3 comprises light source 31 and imageing sensor 32, and they are individually fixed in the both sides up and down of the microchannel detection segment 15 of described micro-fluidic chip, and light source 31, microchannel detection segment 15 and imageing sensor 32 are positioned on the same optical axis;
Display screen 5 is connected with described digital signal processor 4, is used to show check result.Display screen 5 adopts 640 * 480 liquid crystal display.
Power supply 6 is used for providing operating voltage to whole device among Fig. 1.
The serpentine structure that the liquid mixing section 14 of microchannel is S-shaped, its internal diameter can be designed to 5～200 microns, the detection segment 15 of microchannel can be designed to xsect and be circle, triangle, semicircle or polygonal liquid storage tank, the degree of depth of liquid storage tank can be designed to 20～100 microns, preferred 30～60 microns of the degree of depth.
Micro-fluidic chip 1 is made by substrate 16,17 involutions of two printing opacities, form the microstructure that constitutes described microchannel on one of them substrate 17, another substrate 16 is dull and stereotyped, substrate 16 is close to opening one side of the microstructure of substrate 17, behind two substrate 16,17 involutions the open side of described microstructure is sealed formation described microchannel (comprising liquid mixing section 14 and detection segment 15), wherein, substrate 16,17 can adopt PDMS, glass or other macromolecular material to make.
Micro-fluidic chip 1 can be made by PDMS substrate and glass sheet bonding, and wherein the PDMS substrate is made by the microchannel mould, and the microchannel mould adopts the MEMS processing technology to make.Its a kind of concrete manufacturing process is as follows:
1. draw the design configuration (being Micro Channel Architecture figure) of micro-fluidic chip with computerized mapping software.
2. making lay photoetching mask plate
Mask material is selected 5 inches chromium plating glass plates for use, and electron beam exposure is transferred to design configuration on the mask.
3. exposure, development
Clean 4 cun silicon chips, be coated with last layer photoresist (positive glue) on its surface equably, the silicon chip that has photoresist exposes by the transparent part in the mask that is painted with predetermined pattern at a branch of light, and then pattern part has been protected by photoresist.
4. plasma etching (ICP)
The high-energy plasma body of air inclusion molecule, free electron and gaseous ion clashes into silicon chip surface, and removes silicon, 50 microns of etching depths from the surface.So far, the microchannel mould is made and is finished, 50 microns of pattern part projectioies.
5. make the PDMS substrate
PDMS mixes according to mass ratio 10:1 with hardening agent, stirs, and removes bubble, be poured on the mould of microchannel, place baking oven to solidify about 1 hour back and take out cooling with 70 ℃, take the PDMS of shaping off, punch with boring cutter, promptly make the PDMS substrate at passage injection port and liquid outlet.
6. encapsulation
The PDMS substrate is bonded together with glass sheet after the oxygen gas plasma surface treatment, promptly makes micro-fluidic chip.The chip of making as shown in Figure 3.
Micro-injection pump 2 can adopt a twin-channel micro-injection pump, as KDS 200 of U.S. KDScience company etc., also can adopt 2 single pass micro-injection pumps, such as KDS 100 of U.S. KDScience company etc.
Two most important component of the optical detection system of micro-fluidic chip are light source and detector, so the microminiaturization of the optical detection system microminiaturization of light source and these two critical pieces of detector just.Aspect detector microminiaturized, the most effective and direct method is placed directly in micro-fluidic chip on the imaging detection array exactly.
In this screening apparatus, light source 31 adopts led light source, and imageing sensor 32 adopts cmos image sensor, and pixel size can reach 2 μ m.The cmos imaging array can the single pixel of directly address, thereby obtains sensitiveer analysis the integral time that can change each pixel and since in general most of unit of imaging array use less than, so CMOS has the shorter response time.Though the transverse resolution of COMS can not reach the level of optical microscope, they can satisfy the visible light experimental requirements on the larger object fully.The best result of so approximate imaging system distinguishes that power is decided by the pixel size on the imaging array, and it can reach below the 10 μ m.The effective coverage size of pixel is then depended in the sensitivity of imaging system, equally also depends on the leakage current of pixel.
Use the cmos image sensor direct imaging also to possess following advantage: (1) integrated level height, image sensor array, driving and control circuit, signal processing circuit, A/D change-over circuit, digital interface circuit etc. can be integrated, can realize the single-chip imaging system; (2) operating voltage is low, and power consumption is little; (3) lower systematic cost.
The picture signal that cmos image sensor 32 collects can obtain the gray-scale value of corresponding each pixel of image, can determine, can recover original image fully by these gray-scale values.Through the method for similar digital outline, the pixel value difference of the mixed liquor of more Normocellular mixed liquor and cell to be measured on DSP 4, this species diversity has directly reflected the absorbance of mixed liquor, absorbance can be used for diagnosis and use.When making absorbance detection, image processing methods such as use burn into binaryzation carry out edge extracting to the erythrogram in the passage, obtain parameters such as approximate red cell morphology, size, volume.
Testing process is as follows: blood to be measured and certain density salt solusion enter microchannel in the micro-fluidic chip 1 from 11,12 drivings by micro-injection pump 2 of two of micro-fluidic chip inlets respectively, after the liquid mixing section 14 by the microchannel, blood to be measured and salt solusion fully mix, enter detection segment 15 then, the visible light that LED 31 sends is incident on the cmos image sensor 32 by detection segment 15, forms the image information about blood and salt solution mix liquid.These information are handled by DSP 4, compare with the optical characteristics of normal blood, judge whether pathology of blood to be measured, simultaneously, to the red blood cell imaging in the mixed liquor, specifically observe erythrocytic morphological feature by imaging system, judge for the doctor.
Also can open three inlets on micro-fluidic chip 1, one is advanced blood sample, and two other advances solution, forms so-called " extraining sampling formula "; Also can open more inlet, just passable as long as blood sample and solution all enter chip.Outlet also can be opened two or more.
Claims (7)
1, based on the portable thalassemia screening apparatus of micro-fluidic chip image technology, it is characterized in that comprising:
Micro-fluidic chip has at least two inlets and at least one outlet, and the microchannel is set in it, and described microchannel comprises liquid mixing section and detection segment, and the input end of liquid mixing section is communicated with described inlet, and detection segment is communicated with described outlet;
Micro-injection pump is communicated with the inlet of described micro-fluidic chip, is used for blood to be measured and salt solusion are pumped into microchannel in the micro-fluidic chip;
Optical detection device comprises light source and imageing sensor, and they are individually fixed in the both sides up and down of the microchannel detection segment of described micro-fluidic chip, and light source, microchannel detection segment and imageing sensor are positioned on the same optical axis;
Digital signal processor is connected with described imageing sensor, and detected signal is carried out the extraction of gray-scale value and cell edges, needed absorbance value of the luminosity method of inspection that is absorbed and the required cell characteristic value of diagnosis; With,
Display screen is connected with described digital signal processor, is used to show check result.
2, screening apparatus according to claim 1 is characterized in that: the liquid mixing section of described microchannel S-shaped or Z-shaped or the bow font serpentine structure, its internal diameter is 5～200 microns; The detection segment of microchannel is that xsect is circle, triangle, semicircle or polygonal liquid storage tank, and the degree of depth of liquid storage tank is 20～100 microns.
3, screening apparatus according to claim 2 is characterized in that: the degree of depth of described liquid storage tank is 30～60 microns.
4, according to each described screening apparatus of claim 1-3, it is characterized in that: described micro-fluidic chip is made by the substrate involution of two printing opacities, form the microstructure that constitutes described microchannel on one of them substrate, another substrate is close to opening one side of described microstructure, behind two substrate involutions the open side of described microstructure is sealed the described microchannel of formation.
5, according to each described screening apparatus of claim 1-3, it is characterized in that: described micro-fluidic chip is to be made by PDMS substrate and glass sheet bonding, wherein the PDMS substrate is made by the microchannel mould, and the microchannel mould adopts the MEMS processing technology to make.
6, according to each described screening apparatus of claim 1-3, it is characterized in that: described light source is a led light source, and described imageing sensor adopts cmos image sensor.
7, screening apparatus according to claim 6 is characterized in that: the pixel size of described cmos image sensor is 2 μ m.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200810218356 CN101435818B (en) | 2008-12-12 | 2008-12-12 | Portable Mediterranean anemia disease screening apparatus based on micro-flow control chip image technology |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200810218356 CN101435818B (en) | 2008-12-12 | 2008-12-12 | Portable Mediterranean anemia disease screening apparatus based on micro-flow control chip image technology |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101435818A true CN101435818A (en) | 2009-05-20 |
| CN101435818B CN101435818B (en) | 2013-01-09 |
Family
ID=40710363
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200810218356 Expired - Fee Related CN101435818B (en) | 2008-12-12 | 2008-12-12 | Portable Mediterranean anemia disease screening apparatus based on micro-flow control chip image technology |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101435818B (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102352312A (en) * | 2011-07-21 | 2012-02-15 | 西安交通大学 | Integrated system of detection and injection of getting gene to cell and technology thereof |
| CN102559483A (en) * | 2012-01-11 | 2012-07-11 | 浙江大学 | Device and method for real-time monitoring of behaviors and states of cells |
| CN104212705A (en) * | 2014-09-19 | 2014-12-17 | 成都劲宏科技有限公司 | Pulse detection technology based dual-passage cell microflow image acquisition system |
| CN104937411A (en) * | 2012-09-24 | 2015-09-23 | 布里格姆女子医院有限公司 | Portal and method for management of dialysis therapy |
| CN106404641A (en) * | 2016-08-24 | 2017-02-15 | 江苏卓微生物科技有限公司 | Micro particle counting device and method |
| WO2019071918A1 (en) * | 2017-10-11 | 2019-04-18 | 南京大学 | Platelet projection imaging-based detection device and method therefor |
| CN111108365A (en) * | 2017-05-22 | 2020-05-05 | 生物电子公司 | Assay system and method for processing sample entities |
| CN113063744A (en) * | 2021-03-22 | 2021-07-02 | 华东理工大学 | Micro-fluidic device and imaging detection device |
| CN113751092A (en) * | 2021-10-19 | 2021-12-07 | 军事科学院***工程研究院卫勤保障技术研究所 | Silicon-based sensor micro-fluidic chip and preparation and packaging method thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1276094C (en) * | 2002-04-23 | 2006-09-20 | 亚能生物技术（深圳）有限公司 | DNA chip for diagnosing Mediterranean anemia and its prepn |
| CN100557033C (en) * | 2007-04-25 | 2009-11-04 | 亚能生物技术（深圳）有限公司 | Diagnosing alpha-thalassemic nucleic acid film bar and test kit |
-
2008
- 2008-12-12 CN CN 200810218356 patent/CN101435818B/en not_active Expired - Fee Related
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102352312A (en) * | 2011-07-21 | 2012-02-15 | 西安交通大学 | Integrated system of detection and injection of getting gene to cell and technology thereof |
| CN102352312B (en) * | 2011-07-21 | 2013-04-17 | 西安交通大学 | Integrated system of detection and injection of getting gene to cell and technology thereof |
| CN102559483A (en) * | 2012-01-11 | 2012-07-11 | 浙江大学 | Device and method for real-time monitoring of behaviors and states of cells |
| CN104937411A (en) * | 2012-09-24 | 2015-09-23 | 布里格姆女子医院有限公司 | Portal and method for management of dialysis therapy |
| CN104212705A (en) * | 2014-09-19 | 2014-12-17 | 成都劲宏科技有限公司 | Pulse detection technology based dual-passage cell microflow image acquisition system |
| CN104212705B (en) * | 2014-09-19 | 2016-02-10 | 成都劲宏科技有限公司 | A kind of two channels cell microfluidic image capturing system based on impulse detection technique |
| CN106404641A (en) * | 2016-08-24 | 2017-02-15 | 江苏卓微生物科技有限公司 | Micro particle counting device and method |
| CN111108365A (en) * | 2017-05-22 | 2020-05-05 | 生物电子公司 | Assay system and method for processing sample entities |
| WO2019071918A1 (en) * | 2017-10-11 | 2019-04-18 | 南京大学 | Platelet projection imaging-based detection device and method therefor |
| CN109655383A (en) * | 2017-10-11 | 2019-04-19 | 南京大学 | A kind of detection device and its method based on blood platelet projection imaging |
| CN113063744A (en) * | 2021-03-22 | 2021-07-02 | 华东理工大学 | Micro-fluidic device and imaging detection device |
| CN113751092A (en) * | 2021-10-19 | 2021-12-07 | 军事科学院***工程研究院卫勤保障技术研究所 | Silicon-based sensor micro-fluidic chip and preparation and packaging method thereof |
| CN113751092B (en) * | 2021-10-19 | 2023-02-28 | 军事科学院***工程研究院卫勤保障技术研究所 | Silicon-based sensor micro-fluidic chip and preparation and packaging method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101435818B (en) | 2013-01-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101435818B (en) | Portable Mediterranean anemia disease screening apparatus based on micro-flow control chip image technology | |
| CN108700577B (en) | Device and system for analyzing a sample, in particular blood, and method for using same | |
| CN108780081B (en) | Simplified-procedure, small-sample, rapid, easy-to-use bio/chemical analysis apparatus and method | |
| EP3779439B1 (en) | Micro-fluidic chip and analysis instrument having same | |
| CN103323605B (en) | A kind of micro-fluidic chip of saccharification hemoglobin immune detection | |
| DE60035199T2 (en) | ANALYSIS CASSETTE AND LIQUID CONVEYOR CONTROLLER | |
| EP3283883B1 (en) | Lateral flow device, assay device and kit and method for analyzing a fluid sample | |
| SE532499C2 (en) | Method and apparatus for analyzing particles in a liquid sample | |
| KR20060130657A (en) | Analysis element for use in method of testing specimen | |
| CN108801959A (en) | One kind being based on microflow control technique original position ammonia nitrogen on-line computing model | |
| CN101464458B (en) | Micro-flow controlled chip used for detecting erythrocyte osmotic fragility | |
| CN108435266A (en) | A kind of micro-fluidic detection chip and the kit based on it, whole blood multiple determination methods and applications | |
| AU2015298499B2 (en) | Microfluidic water sample analysis kit | |
| JP2009517650A (en) | Microfluidic device with porous membrane and unbranched channel | |
| US20210123903A1 (en) | Micro-fluidic Chip and Analytical Instrument Provided with the Micro-fluidic Chip | |
| EP1717585B1 (en) | Microchip and analysis method using the same | |
| JP5650970B2 (en) | Inspection apparatus, control method therefor, and reaction container for inspection | |
| WO2006006113A1 (en) | Microlaboratory for biological fluids analysis using white light illumnation | |
| CN211402400U (en) | Exosome identification device | |
| Zuo et al. | An integrated microfluidic system for multi-target biochemical analysis of a single drop of blood | |
| CN109900889A (en) | A kind of three detection cards of automatic hemofiltration dry type photochemical method microchannel liver function | |
| CN208537406U (en) | One kind being based on microflow control technique original position ammonia nitrogen on-line computing model | |
| CN107121421A (en) | Portable range estimation luminoscope and method for heavy metal ion in Site Detection water sample | |
| EP3908821A1 (en) | Blood separation and analysis device and methods | |
| Zhou et al. | Development and prospects of microfluidic platforms for sperm inspection |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130109 Termination date: 20171212 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |