CN104280328A - Flow cytometry analysis device and method - Google Patents
Flow cytometry analysis device and method Download PDFInfo
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Abstract
The invention discloses a flow cytometry analysis device and method. The analysis device comprises a loading detection device, a sheath fluid device and a pressure supply device, wherein the loading detection device comprises a sample container, a loading pipeline, a flow detector and a flow chamber; the sample container is used for containing a sample solution and is sealed by virtue of a sealing piece; one end of the loading pipeline is connected with the sample container, the flow detector is arranged on the loading pipeline, and the flow chamber is connected with the other end of the loading pipeline; the sheath fluid device comprises a sheath fluid container, a sheath fluid pipeline and a first valve device; one end of the sheath fluid pipeline is connected with the sheath fluid container; the first valve device is arranged on the sheath fluid pipeline; the pressure supply device comprises an air source, a first air pressure pipeline and a second air pressure pipeline; the first air pressure pipeline is connected between the air source and the sample container; and the second air pressure pipeline is connected between the air source and the sheath fluid container. According to the flow cytometry analysis device provided by the invention, the problem of low analysis accuracy is solved on the basis of low cost and convenience in use.
Description
Technical field
The present invention relates to cell analysis technical field, particularly relate to a kind of flow cytometry device and analytical approach.
Background technology
Streaming is generally used to focus on art in blood cell/field of cell analysis, be constrained to unicellular stream by sample cell, through sensor, irradiate detection or electric field between electrodes impedance variation by laser, produce the pulse of different amplitude and quantity, thus cell is counted and the analysis of the information such as size.
The central principle that streaming focuses on art is pipeline lamina flow principle, and fluid is by two parts: sample stream and sheath stream form, and flows through sensor, clear-cut between its mesotheca stream and sample stream at pipeline mesotheca stream parcel sample, and meets the velocity gradient distribution of flow measurement.
Flow cytometry device of the prior art adopts syringe pump or peristaltic pump to drive sample stream and sheath stream usually.But, adopt syringe pump or peristaltic pump easily to cause the fluctuation of sheath hydraulic coupling, cause the fluctuation of sheath flow quantity, and then affect sample stream stability.
In addition, flow cytometry device of the prior art, usually uses normalized particle mensuration, namely adds the particle of concentration known in the sample, mix with the sample of known volume by the standard particle of concentration known, the standard particle concentration in mixed liquor can calculate.Machine on mixed liquor is measured, draws standard particle number, measured body accumulated amount can be gone out by inverse, reach the object of blood cell absolute counting.But this method by concentration known normalized particle inverse sample volume needs user accurately to allot the concentration of particle, require higher to user, use inconvenience, and add the use cost of user.
Therefore, how can solve the lower and object in order to realize blood cell absolute counting of the precision of analysis that causes of Almost Sure Sample Stability difference simultaneously, and cause cost to raise and awkward problem, become those skilled in the art's technical matters to be solved.
Summary of the invention
The invention provides a kind of flow cytometry device, it on wananty costs basis lower and easy to use, can solve the problem that precision of analysis is lower.Present invention also offers a kind of flow cytometric analysis.
A kind of flow cytometry device provided by the invention, comprising:
Sample detection device, described sample detection device comprises for accommodating sample liquid and the sample container of being undertaken sealing by seal, loading pipeline that one end is connected with described sample container, the flow chamber that is arranged at the flow detection meter on described loading pipeline and is connected with the other end of described loading pipeline, sample liquid forms sample stream in described flow chamber, and described flow chamber is provided with sample flow analysis device;
Sheath liquid device, the first valving that described sheath liquid device comprises sheath liquid container, sheath liquid pipeline that one end is connected with described sheath liquid container and is arranged on described sheath liquid pipeline, the other end of described sheath liquid pipeline is connected with described flow chamber, forms described sample stream to make sheath fluid and sample liquid in described flow chamber;
Pressure supply unit, the second pneumatic circuit that described pressure supply unit comprises source of the gas, is connected to the first pneumatic circuit between described source of the gas and described sample container and is connected between described source of the gas and described sheath liquid container, described first pneumatic circuit is provided with loading pressure adjustment assembly and loading pressure transducer, and described second pneumatic circuit is provided with sheath fluid pressure adjustment assembly and sheath fluid pressure transducer.
Preferably, described seal is cleaning swab, and described loading pipeline passes described cleaning swab by loading pin, and described cleaning swab and described loading pin are that slipper seal coordinates; Described flow cytometry device also comprises cleaning device, and described cleaning device comprises the detergent line be connected between described cleaning swab and described sheath liquid container, and described detergent line is provided with the second valving.
The 3rd valving and the waste drains pump preferably, also comprising waste liquid recovery apparatus, described waste liquid recovery apparatus comprises waste liquid barrel, be connected to the first waste-solution line between described cleaning swab and described waste liquid barrel, be arranged at described first waste-solution line.
Preferably, described waste liquid recovery apparatus also comprises waste liquid pool, be connected to the second waste-solution line between described flow chamber and described waste liquid pool and be arranged at the 4th valving of described second waste-solution line, described second waste-solution line is connected with described first waste-solution line, and tie point is between described 3rd valving and described waste drains pump.
Present invention also offers a kind of flow cytometric analysis, comprise step:
Loading pressure and sheath hydraulic coupling are provided, and control described loading pressure and be greater than described sheath hydraulic coupling, to make sample liquid flow in sheath fluid, form sample stream;
Regulate the original pressure of loading pressure, make loading pressure remain in setting range, realize the coarse adjustment of loading pressure;
The real-time traffic and the target setting value that sample stream are flow to flow chamber compare, if described real-time traffic is greater than described target setting value, then control described loading pressure drop, level off to described target setting value to make described real-time traffic; If described real-time traffic is less than described target setting value, then control described loading pressure increase, level off to described target setting value to make described real-time traffic;
Different time in each step above-mentioned obtains the real-time traffic that sample liquid flow to flow chamber;
The flow summation that sample liquid flow to flow chamber is calculated, to calculate cell concentration by the computing method of integration.
A kind of flow cytometry device provided by the invention, comprise: sample detection device, described sample detection device comprises for accommodating sample liquid and the sample container of being undertaken sealing by seal, loading pipeline that one end is connected with described sample container, the flow chamber that is arranged at the flow detection meter on described loading pipeline and is connected with the other end of described loading pipeline, sample liquid forms sample stream in described flow chamber, and described flow chamber is provided with sample flow analysis device; Sheath liquid device, the first valving that described sheath liquid device comprises sheath liquid container, sheath liquid pipeline that one end is connected with described sheath liquid container and is arranged on described sheath liquid pipeline, the other end of described sheath liquid pipeline is connected with described flow chamber, forms described sample stream to make sheath fluid and sample liquid in described flow chamber; Pressure supply unit, the second pneumatic circuit that described pressure supply unit comprises source of the gas, is connected to the first pneumatic circuit between described source of the gas and described sample container and is connected between described source of the gas and described sheath liquid container, described first pneumatic circuit is provided with loading pressure adjustment assembly and loading pressure transducer, and described second pneumatic circuit is provided with sheath fluid pressure adjustment assembly and loading pressure transducer.
Setting like this, flow cytometry device provided by the invention, sample liquid can be delivered to flow chamber according to target flow, is comprised by sheath fluid, is formed and stablizes sample stream to be checked, described in specific as follows.Source of the gas provides loading pressure by the first pneumatic circuit to sample container, sheath hydraulic coupling is provided to sheath liquid container by the second pneumatic circuit, flow under pressure in flow chamber to make sample liquid and sheath fluid, form sample stream, loading pressure adjustment assembly and sheath fluid pressure adjustment assembly can regulate respectively to loading pressure and sheath hydraulic coupling, during loading, control loading pressure by loading pressure adjustment assembly and sheath fluid pressure adjustment assembly and be greater than sheath hydraulic coupling, to make sample liquid flow in sheath fluid, form sample stream; Owing to starting the loading stage, the loading pressure of sample liquid is comparatively large, and it is greater than set target pressure, forms the effect pushing away sample fast.Then, loading pressure transducer detects loading pressure, and is compared by the actual flow value and target flow value comparing the sample flow detection meter detection on loading pipeline, if actual flow value is greater than target flow value, then controls loading pressure drop; If actual flow value is less than target flow value, then control loading pressure increase; Final adjustment loading pressure makes actual flow value level off to target flow value.The loading pressure of loading pressure transducer real-time detection practical, analyst can Real-Time Monitoring loading pressure, and loading pressure is remained in normal range.So ensure that the stability of loading flow, in addition, flow in the process of flow chamber at sample liquid, flow detection meter periodically can obtain real-time traffic, the flow value that sample flow analysis device repeatedly obtains according to flow detection meter, according to integral, the total flow in special time can be calculated, and then sample flow analysis device accurately can calculate cell concentration according to the total flow of sample liquid.Setting like this, sample flow quantity obtains when stable, and the cell concentration analyzed has higher accuracy.And, cell analysis apparatus provided by the invention, adopt source of the gas as loading pressure source and sheath fluid pressure source, avoid the parts of the pulse adopting high precision pump of the prior art and cancellation pump to produce, therefore, flow cytometry device provided by the invention, it on wananty costs basis lower and easy to use, can solve the problem that analysis precision is lower.
Present invention also offers a kind of flow cytometric analysis, comprise step:
Loading pressure and sheath hydraulic coupling are provided, and control described loading pressure and be greater than described sheath hydraulic coupling, to make sample liquid flow in sheath fluid, form sample stream;
Regulate the original pressure of loading pressure, make loading pressure remain in setting range, realize the coarse adjustment of loading pressure;
The real-time traffic and the target setting value that sample stream are flow to flow chamber compare, if described real-time traffic is greater than described target setting value, then control described loading pressure drop, level off to described target setting value to make described real-time traffic; If described real-time traffic is less than described target setting value, then control described loading pressure increase, level off to described target setting value to make described real-time traffic.
Different time in each step above-mentioned obtains the real-time traffic that sample liquid flow to flow chamber;
The flow summation that sample liquid flow to flow chamber is calculated, to calculate cell concentration by the computing method of integration.
So, according to cell analysis method provided by the invention, the stability of the flow of sample liquid can be ensured, and the flow summation adopting the method for integration to calculate is more accurate, and then ensure that the degree of accuracy of cell concentration.
Accompanying drawing explanation
In order to be illustrated more clearly in the embodiment of the present invention or technical scheme of the prior art, be briefly described to the accompanying drawing used required in embodiment or description of the prior art below, apparently, accompanying drawing in the following describes is only some embodiments of the present invention, for those of ordinary skill in the art, under the prerequisite not paying creative work, other accompanying drawing can also be obtained according to these accompanying drawings.
Fig. 1 is the flow cytometry device schematic diagram that the specific embodiment of the invention provides;
In figure:
Sample container-11, loading pipeline-12, flow detection meter-13, flow chamber-14, sheath liquid container-15, sheath liquid pipeline-16, first valving-17, source of the gas-18, first pneumatic circuit-19, second pneumatic circuit-20, loading pressure adjustment assembly-21, loading pressure transducer-22, sheath fluid pressure adjustment assembly-23, sheath fluid pressure transducer-24, cleaning swab-25, detergent line-26, second valving-27, waste liquid barrel-28, first waste-solution line-29, 3rd valving-30, waste drains pump-31, waste liquid pool-32, second waste-solution line-33, 4th valving-34.
Embodiment
This embodiment provides a kind of flow cytometry device, and it on wananty costs basis lower and easy to use, can solve the problem that precision of analysis is lower.Present invention also offers a kind of flow cytometric analysis.
Below in conjunction with the accompanying drawing in the embodiment of the present invention, be clearly and completely described the technical scheme in the embodiment of the present invention, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
Please refer to Fig. 1, the flow cytometry device that this embodiment provides, comprising:
Sample detection device, sample detection device comprises for accommodating sample liquid and the sample container 11 of being undertaken sealing by seal, loading pipeline 12 that one end is connected with sample container 11, the flow chamber 14 that is arranged at the flow detection meter 13 on loading pipeline 12 and is connected with the other end of loading pipeline 12, sample liquid forms sample stream in flow chamber 14, and flow chamber 14 is provided with sample flow analysis device;
Sheath liquid device, the sheath liquid pipeline 16 that sheath liquid device comprises sheath liquid container 15, one end is connected with sheath liquid container 15 and the first valving 17 be arranged on sheath liquid pipeline 16, the other end of sheath liquid pipeline 16 is connected with flow chamber 14, forms sample stream to make sheath fluid and sample liquid in flow chamber 14;
Pressure supply unit, the second pneumatic circuit 20 that pressure supply unit comprises source of the gas 18, is connected to the first pneumatic circuit 19 between source of the gas 18 and sample container 11 and is connected between source of the gas 18 and sheath liquid container 15, first pneumatic circuit 19 is provided with loading pressure adjustment assembly 21 and loading pressure transducer 22, and described second pneumatic circuit 20 is provided with sheath fluid pressure adjustment assembly 23 and sheath fluid pressure transducer 24.
It should be noted that, above-mentioned loading pressure adjustment assembly 21 and sheath fluid pressure adjustment assembly 23 can be air pressure regulator.
Setting like this, the flow cytometry device that this embodiment provides, sample liquid can be delivered to flow chamber 14 according to target flow, is comprised by sheath fluid, is formed and stablizes sample stream to be checked.Source of the gas 18 provides loading pressure by the first pneumatic circuit 19 to sample container 11, sheath hydraulic coupling is provided to sheath liquid container 15 by the second pneumatic circuit 20, flow in flow chamber 14 under pressure to make sample liquid and sheath fluid, form sample stream, loading pressure adjustment assembly 21 and sheath fluid pressure adjustment assembly 23 can regulate respectively to loading pressure and sheath hydraulic coupling, during loading, control loading pressure by loading pressure adjustment assembly 21 and sheath fluid pressure adjustment assembly 23 and be greater than sheath hydraulic coupling, to make sample liquid flow in sheath fluid, form sample stream.
Owing to starting the loading stage, the loading pressure of sample liquid is comparatively large, and it is greater than set target pressure, forms the effect pushing away sample fast.Then, loading pressure transducer detects loading pressure, and is compared by the actual flow value and target flow value comparing the sample flow detection meter detection on loading pipeline, if actual flow value is greater than target flow value, then controls loading pressure drop; If actual flow value is less than target flow value, then control loading pressure increase; Final adjustment loading pressure makes actual flow value level off to target flow value.The loading pressure of loading pressure transducer real-time detection practical, analyst can Real-Time Monitoring loading pressure, and loading pressure is remained in normal range.So ensure that the stability of loading flow, in addition, flow in the process of flow chamber 14 at sample liquid, flow detection meter 13 periodically can obtain real-time traffic, the flow value that sample flow analysis device obtains for more than 13 time according to flow detection meter, according to integral, can calculate the total flow in special time, and then sample flow analysis device accurately can calculate cell concentration according to the total flow of sample liquid, specifically can be as described below:
In this embodiment, flow detection meter 13 can detect sample flow quantity scope from a few microliter per minute to hundreds of microliter per minute, and therefore, when sample liquid flows through flow detection meter 13, flow detection meter 13 can obtain the flow of current sample liquid; When starting to measure, trigger recording sample flow value Q, and with the periodicity time, such as, the 0.1s time interval is timeslice T, sample actual flow Q when recording each timeslice, when system log (SYSLOG) completes, integration adds up the flow of each timeslice, and final calculated population amasss Vs, algorithm is as follows: Vs=V1+V2+ ... + Vt=(Q1+Q2+ ... + Qt) T=0.1 × (Q1+Q2+ ... + Qt), unit is microlitre; If when time record cell (particle) adds up to A (individual), then absolute counting calculating concentration result N is: N=A/ (Vs × 10
-6), unit is individual | rises; If sample before measuring by dilution B doubly, then concentration N '=BN, unit is individual | rises.
Setting like this, sample flow quantity obtains when stable, and the cell concentration analyzed has higher accuracy.And, the cell analysis apparatus that this embodiment provides, adopt source of the gas 18 as loading pressure source and sheath fluid pressure source, avoid the parts of the pulse adopting high precision pump of the prior art and cancellation pump to produce, therefore, flow cytometry device provided by the invention, it on wananty costs basis lower and easy to use, can solve the problem that analysis precision is lower.
In addition, in the preferred version that this embodiment provides, sample container 11 is provided with the cleaning swab 25 be sealed and matched with it, and loading pipeline 12 passes cleaning swab 25 by loading pin, and cleaning swab 25 is that slipper seal coordinates with loading pin; Flow cytometry device also comprises cleaning device, and cleaning device comprises the detergent line 26 be connected between cleaning swab 25 and sheath liquid container 15, and detergent line 26 is provided with the second valving 27.
Setting like this, cleaning swab 25 is that slipper seal coordinates with loading pin, avoids the problem of sample container 11 pressure release, and cleaning swab 25 can slide along loading pin, can clean loading pin.During cleaning, open the second valving 27 and sheath fluid pressure adjustment assembly 23, sheath fluid flow to cleaning swab 25 by detergent line 26, and cleaning swab 25 slides along loading pin, to clean the outer wall of loading pin.
In addition, the flow cytometry device that this embodiment provides, can also comprise waste liquid recovery apparatus, described waste liquid recovery apparatus comprises waste liquid barrel 28, is connected to the first waste-solution line 29, the 3rd valving 30 being arranged at the first waste-solution line 29 and the waste drains pump 31 that clean between swab 25 and waste liquid barrel 28.
Setting like this, when cleaning the outer wall of loading pin, can open the 3rd valving 30 and waste drains pump 31, waste drains pump 31 provides negative-pressure gas simultaneously, can be evacuated among waste liquid barrel 28 by the waste liquid after cleaning loading pin, reclaim the waste liquid after cleaning.
Further, waste liquid recovery apparatus can also comprise waste liquid pool 32, be connected to the second waste-solution line 33 between flow chamber 14 and waste liquid pool 32 and be arranged at the 4th valving 34 of the second waste-solution line 33, second waste-solution line 33 is connected with the first waste-solution line 29, and tie point is between the 3rd valving 30 and waste drains pump 31.
Setting like this, after measurement terminates, ends the first pneumatic circuit 19 and sheath liquid pipeline 16 simultaneously, and opens the 3rd valving 30, now, and earth pressure release in sample container 11; Then setting sheath fluid pressure adjustment assembly 23 to cleaning pressure is opened, open waste drains pump 31 and the second valving 27, and now clean swab 25 to move downward, due to the suction function of waste drains pump 31, form the gas-liquid mixture of cleaning loading pin in cleaning swab 25, descending process is fully cleaned loading pin outer wall; When cleaning swab 25 arrives the most advanced and sophisticated position of loading pin, close the second valving 27, and open the first valving 17, now, the inwall of flow chamber 14 and loading pin, flow detection meter 13 is cleaned, cleaning waste liquid by waste drains pump 31 through the 3rd valving 30 pump drainage to waste liquid barrel 28.This kind of cleaning structure and method can with relative few consumption liquid measure and the high-efficiency washings completed pin consuming time.
This embodiment additionally provides a kind of flow cytometric analysis, comprises step:
Loading pressure and sheath hydraulic coupling are provided, and control described loading pressure and be greater than described sheath hydraulic coupling, to make sample liquid flow in sheath fluid, form sample stream;
Regulate the original pressure of loading pressure, make loading pressure remain in setting range, realize the coarse adjustment of loading pressure;
The real-time traffic and the target setting value that sample stream are flow to flow chamber compare, if described real-time traffic is greater than described target setting value, then control described loading pressure drop, level off to described target setting value to make described real-time traffic; If described real-time traffic is less than described target setting value, then control described loading pressure increase, level off to described target setting value to make described real-time traffic;
Different time in each step above-mentioned obtains the real-time traffic that sample liquid flow to flow chamber;
The flow summation that sample liquid flow to flow chamber is calculated, to calculate cell concentration by the computing method of integration.
So, the cell analysis method provided according to this embodiment, can ensure the stability of the flow of sample liquid, and the flow summation adopting the method for integration to calculate is more accurate, and then ensure that the degree of accuracy of cell concentration.
To the above-mentioned explanation of the disclosed embodiments, professional and technical personnel in the field are realized or uses the present invention.To be apparent for those skilled in the art to the multiple amendment of these embodiments, General Principle as defined herein can without departing from the spirit or scope of the present invention, realize in other embodiments.Therefore, the present invention can not be restricted to these embodiments shown in this article, but will meet the widest scope consistent with principle disclosed herein and features of novelty.
Claims (5)
1. a flow cytometry device, is characterized in that, comprising:
Sample detection device, described sample detection device comprises for accommodating sample liquid and the sample container of being undertaken sealing by seal, loading pipeline that one end is connected with described sample container, the flow chamber that is arranged at the flow detection meter on described loading pipeline and is connected with the other end of described loading pipeline, sample liquid forms sample stream in described flow chamber, and described flow chamber is provided with sample flow analysis device;
Sheath liquid device, the first valving that described sheath liquid device comprises sheath liquid container, sheath liquid pipeline that one end is connected with described sheath liquid container and is arranged on described sheath liquid pipeline, the other end of described sheath liquid pipeline is connected with described flow chamber, forms described sample stream to make sheath fluid and sample liquid in described flow chamber;
Pressure supply unit, the second pneumatic circuit that described pressure supply unit comprises source of the gas, is connected to the first pneumatic circuit between described source of the gas and described sample container and is connected between described source of the gas and described sheath liquid container, described first pneumatic circuit is provided with loading pressure adjustment assembly and loading pressure transducer, and described second pneumatic circuit is provided with sheath fluid pressure adjustment assembly and sheath fluid pressure transducer.
2. flow cytometry device as claimed in claim 1, is characterized in that, described seal is cleaning swab, and described loading pipeline passes described cleaning swab by loading pin, and described cleaning swab and described loading pin are that slipper seal coordinates; Described flow cytometry device also comprises cleaning device, and described cleaning device comprises the detergent line be connected between described cleaning swab and described sheath liquid container, and described detergent line is provided with the second valving.
3. flow cytometry device as claimed in claim 2, it is characterized in that, the 3rd valving and the waste drains pump also comprising waste liquid recovery apparatus, described waste liquid recovery apparatus comprises waste liquid barrel, be connected to the first waste-solution line between described cleaning swab and described waste liquid barrel, be arranged at described first waste-solution line.
4. flow cytometry device as claimed in claim 3, it is characterized in that, described waste liquid recovery apparatus also comprises waste liquid pool, be connected to the second waste-solution line between described flow chamber and described waste liquid pool and be arranged at the 4th valving of described second waste-solution line, described second waste-solution line is connected with described first waste-solution line, and tie point is between described 3rd valving and described waste drains pump.
5. a flow cytometric analysis, is characterized in that, comprises step:
Loading pressure and sheath hydraulic coupling are provided, and control described loading pressure and be greater than described sheath hydraulic coupling, to make sample liquid flow in sheath fluid, form sample stream;
Regulate the original pressure of loading pressure, make loading pressure remain in setting range, realize the coarse adjustment of loading pressure;
The real-time traffic and the target setting value that sample stream are flow to flow chamber compare, if described real-time traffic is greater than described target setting value, then control described loading pressure drop, level off to described target setting value to make described real-time traffic; If described real-time traffic is less than described target setting value, then control described loading pressure increase, level off to described target setting value to make described real-time traffic;
Different time in each step above-mentioned obtains the real-time traffic that sample liquid flow to flow chamber;
The flow summation that sample liquid flow to flow chamber is calculated, to calculate cell concentration by the computing method of integration.
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| CN108663303A (en) * | 2018-07-27 | 2018-10-16 | 北京指真生物科技有限公司 | A kind of blood analyser |
| CN110031386A (en) * | 2019-05-16 | 2019-07-19 | 重庆博奥新景医学科技有限公司 | A kind of flow cytometer liquid channel system and its detection method |
| CN110031386B (en) * | 2019-05-16 | 2023-12-26 | 重庆博奥新景医学科技有限公司 | Flow cytometer liquid path system and detection method thereof |
| CN110118717A (en) * | 2019-05-28 | 2019-08-13 | 北京唯公医疗技术有限公司 | The liquid channel system and its application method and flow cytometer of flow cytometer |
| CN110118716A (en) * | 2019-05-28 | 2019-08-13 | 北京唯公医疗技术有限公司 | Liquid fluid system, air-tightness detection method and the flow cytometer of flow cytometer |
| CN110118718A (en) * | 2019-05-28 | 2019-08-13 | 深圳唯公生物科技有限公司 | Liquid fluid system, test method, the regulation method of negative pressure device and flow cytometer |
| CN110749535A (en) * | 2019-10-15 | 2020-02-04 | 迈克医疗电子有限公司 | Flow cytometry detection system and method |
| CN110749535B (en) * | 2019-10-15 | 2022-02-01 | 迈克医疗电子有限公司 | Flow cytometry detection system and method |
| CN113640194A (en) * | 2021-08-06 | 2021-11-12 | 天津迈科隆生物科技有限公司 | Blood analysis device |
| CN116337729A (en) * | 2023-05-31 | 2023-06-27 | 深圳市帝迈生物技术有限公司 | Blood cell analysis equipment |
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