CN106348884A - Production method of bacillus megaterium and humic acid containing liquid water-soluble fertilizer - Google Patents
Production method of bacillus megaterium and humic acid containing liquid water-soluble fertilizer Download PDFInfo
- Publication number
- CN106348884A CN106348884A CN201610776115.9A CN201610776115A CN106348884A CN 106348884 A CN106348884 A CN 106348884A CN 201610776115 A CN201610776115 A CN 201610776115A CN 106348884 A CN106348884 A CN 106348884A
- Authority
- CN
- China
- Prior art keywords
- product
- bacillus megaterium
- mass ratio
- liquid
- temperature
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Pest Control & Pesticides (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Fertilizers (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to a production method of a bacillus megaterium and humic acid containing liquid water-soluble fertilizer. The production method comprises the following steps: grinding dried algae into powder, adding the alga powder into water according to a certain ratio, putting into a fermenting tank, and sterilizing; inoculating a beef extract-peptone liquid culture medium with a fermenting strain YIC-BM1, and culturing overnight to obtain seed liquid; adding the seed liquid into the fermenting tank according to a certain mass ratio, fermenting, adjusting a fermenting condition, keeping for a certain time, adjusting the fermenting condition again and supplementing the seed liquid, adjusting for three times to obtain bacillus megaterium, adding water, bacillus megaterium liquid, a nitrogen fertilizer, a phosphate fertilizer, a potassium fertilizer and a humic acid-containing organic matter into a stirring tank, and stirring for dissolving completely to obtain the bacillus megaterium and humic acid containing liquid water-soluble fertilizer. By the production method, the fermenting process is stable and controllable, the fermenting cycle is short, and the product efficacy is remarkable; the production method is suitable for large-scale production.
Description
Technical field
The present invention relates to a kind of production method of liquid Water soluble fertilizer.
Background technology
Marine alga fertilizer, with natural seaweed as raw material, is processed through special biochemical process, extracts and retain marine alga natural activity
Composition, a kind of novel organic fertilizer material that refine concentrates and formed.Marine alga fertilizer has raising crop yield and quality, resists disease pest
Effect that evil and poor environment and improved soil conserve water and soil, it is applied to agricultural production the huge market demand and good
Development prospect.But, the quality of marine alga fertilizer to be ensured, it is necessary for getting hold of Sargassum raw material and the crucial ring of extraction process two
Section.
First, the quality of marine alga fertilizer quality depends on raw material used.At present, the raw material used by alga fertilizer manufacturing enterprise
Mainly based on Brown algae, including yellow tang, Thallus Laminariae (Thallus Eckloniae), Thallus Laminariae (Thallus Eckloniae), Alga Sgrgassi Enervess etc..Other algae such as Fucus Vesiculosus, Entermorpha, ulva, Kappa
Zao Dengyeyou minority enterprise with.External alga fertilizer enterprise is most to be raw material using Brown algae, is wherein especially applied with yellow tang
The most extensive such as famous brand name such as seasol, maxicrop, practical function effect also indicates that yellow tang is the Sargassum raw material of high-quality.
But, China coast does not have yellow tang to be distributed, and the raw material type that domestic enterprise adopts is numerous and diverse, and quality is irregular to differ, and causes Sargassum
Fertilizer product quality is very different, or even some producers are turned on the camouflage of " alga fertilizer ", directly blends alginic acid, fulvic acid, nitrogen phosphorus
Potassium etc. pretends to be alga fertilizer, have impact on alga fertilizer quality, has upset alga fertilizer market.
Secondly, the second factor of decision alga fertilizer quality is extraction process.Different extraction processes is to alga fertilizer activity
The species and content impact of composition is huge.At present, the extracting method of Sargassum active component mainly has chemical method, Physical and life
Thing method.Chemical method mainly uses acid, alkali and organic solvent and processes alginic cell, so that cell is cleared up or endogenous substance solubilising, should
Method is simple to operate, easily realizes, and is also the method that most alga fertilizer manufacturing enterprises adopt both at home and abroad.But, chemistry examination
Agent is sizable to the destruction of the active material ingredients in alginic cell, and the organic reagent remaining is also potential to environment
Harm.The principle of Physical is control pressure, the physical environmental condition such as temperature, so that alginic cell is crushed using mechanical force, interior
Tolerant release.Although the method technique cleaning, environmental friendliness, the requirement for instrument and equipment is harsh, change in reaction conditions
Acutely, relatively costly it is difficult to realize large-scale production.Bioanalysises include two methods of enzymic degradation and fermentable, its master
Principle is wanted to be to destroy the small-molecule substance that alginic cell wall construction macromolecular substances of degrading easily absorb, the party for plant
Method reaction is gentle, product diversification, and whole production process safety and environmental protection is pollution-free, is preferable Sargassum fertilizer production method.
Research using Production by Microorganism Fermentation marine alga fertilizer is relatively fewer, and only several researchs are from multiple micro-
Biology carries out similar compost fermentation to Sargassum raw material or aerobic fermentation is processed, and there is sweat poor controllability, fermentation period is long,
The problems such as target product is indefinite is it is difficult to meet the unification of industrialization production requirements, stable, controlled standard, the quality of product
It is difficult to ensure.
Evaluation index for agricultural micro organism mainly includes the microbial inoculum quantity in the purity and unit mass of microbial inoculum.Cause
This, the bacterial preparation process as prior art is usually: carries out once under the conditions of the temperature of the suitable existence of microorganism and ph
Fermentation technology, is equivalent to hot-house culture.
Because the acidifying of current soil compaction is serious, outside air temperature is changeable, and the microbial bacterial agent of hot-house culture is directly thrown
It is put in soil, the soil environment because not adapting to severe causes mortality, even if having surviving because population cannot be formed on a small quantity
Effect, also cannot play the effect of microbial bacterial agent.
Content of the invention
It is water-soluble that the technical problem to be solved is to provide a kind of liquid containing bacillus megaterium and humic acidss
Fertile production method, first, Sargassum of being degraded using the bacillus megaterium of function admirable, and simulate soil natural ecological environment,
Make the bacillus megaterium after fermentation after putting into soil, environment can be adapted to quickly it is ensured that survival rate；Secondth, provide and utilize institute
State the method that bacillus megaterium prepares liquid Water soluble fertilizer, the method sweat is stablized controlled, and fermentation period is short, product efficiency
Significantly, it is suitable to large-scale production.
In order to solve above-mentioned technical problem, the present invention employs the following technical solutions:
A kind of production method of the liquid Water soluble fertilizer containing bacillus megaterium and humic acidss is it is characterised in that according to following
Step is carried out: by water, nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, Organic substance containing humic acidss and bacillus megaterium filtrate according to mass ratio 30～
75:5～40:5～40:5～40:5～40:1～80 are added in stirred tank, after stirring and dissolving, obtain containing huge spore bar
The liquid Water soluble fertilizer of bacterium and humic acidss；
Wherein Organic substance containing humic acidss is the two of one of potassium humate, sodium humate and potassium fulvate or arbitrary proportion
More than kind；
Wherein bacillus megaterium filtrate is prepared from according to following steps:
1), prepare marine algae powder: the Sargassum after drying is clayed into power；
2), prepare product a: marine algae powder and water and load in fermentation tank according to mass ratio 1～10:100, at 100～150 DEG C
Under the conditions of sterilize 15～35min, obtain product a；
And prepare seed liquor: bacillus megaterium yic～bm1 is seeded to fluid medium, in 25～40 degree of cultures 20
Obtain seed liquor within～30 hours；
Volume ratio between bacillus megaterium and culture medium is 1～10:100；
3), prepare product b: seed liquor is added in product a and obtains product b, wherein the mass ratio of seed liquor and product a
For 1～10:100；
4), prepare product c: be 25 DEG C～40 DEG C in temperature, under conditions of ph5～9, product b fermented, during fermentation
Between be 6～10 days, obtain product c；
5), prepare product d: the ph to 5.0～6.0 of adjustment product c, temperature, to 3～10 DEG C, preserves 3h～72h, produced
Product d；
6), prepare product e: the temperature of adjustment product d to 25 DEG C～40 DEG C, ph to 5～9, then supplements seed liquor, supplements
The mass ratio of seed liquor and product d be 1～10:100, preserve 3h～72h, obtain product e；
7), prepare product f: the ph to 5.0～6.0 of adjustment product e, temperature, to 3～10 DEG C, preserves 3h～72h, produced
Product f；
8), prepare product g: the temperature of adjustment product f to 25 DEG C～40 DEG C, ph to 5～9, then supplements seed liquor, supplements
The mass ratio of seed liquor and product f be 1～10:100, preserve 3h～72h, obtain product g；
9), prepare bacillus megaterium filtrate: obtain bacillus megaterium filtrate by after product g filter pressing.
Described fluid medium is made into according to following mass ratio: 5 parts of sodium alginate, 5 parts of ammonium sulfate, 1 part of magnesium sulfate, phosphoric acid
2 parts of hydrogen dipotassium, 0.01 part of ferrous sulfate and 1000 parts of distilled water.
Step 2) mass ratio of described marine algae powder and water is 4:100；115 DEG C of sterilising temp, sterilization time 25min；System
During standby seed liquor, bacillus megaterium is seeded in fluid medium, and culture obtains seed liquor for 22 hours, bacillus megaterium and
The volume ratio of culture medium is 5:100；Step 5) and step 7) in, temperature is 5 DEG C, and the holding time is 12h.
Step 4), step 6) and step 8) in, temperature be 25 DEG C, ph be 6.5～7.0；Step 4) fermentation time be 8 days；
Step 6) and step 8) holding time be 12h；Step 6) in the mass ratio of fermentation seed liquid and product d and step 8) in fermentation
Seed liquor is 5:100 with the mass ratio of product f.
Step 5), step 6) step 7) and step 8) in, adjustment temperature be per hour adjust 3 DEG C.
Described Sargassum refer to two kinds of sargassum thunbergii, Thallus Laminariae (Thallus Eckloniae), opotism, one of Sargassum phyllocystum Tseng et Lu,Sargassum horneri (Turn.) C. Ag. (Fucus horneri (Turn.)C.Ag.,Spongocarpus horneri Kutz.) and Alga Sgrgassi Enervess or arbitrary proportion with
On.
Described bacillus megaterium is bacillus megaterium yic～bm1, and preserving number is cgmcc no.12156.
Invent and there are following Advantageous Effects:
1st, sweat of the present invention is stablized controlled, and fermentation period is short, and product efficiency is notable, is suitable to large-scale production.
2nd, the fermentation liquid that the present invention obtains has good water solubility it is easy to plant absorption utilizes, simultaneously as a kind of important
Signaling molecule, is capable of growth regulating and the induction resistance mechanism of involved in plant, promotes the growth of plant, improve plant to bad ring
Border and the resistance of pest and disease damage.
3rd, the present invention makes the bacterium that fermentation goes out possess the ability adapting to severe soil environment by adjusting fermentation condition, more has
Performance beneficial to bacterium function.
4th, the present invention is by slow temperature adjustment, makes the change of bacillus megaterium gradually adaptive temperature, it is to avoid temperature
Change is too fast, and bacillus megaterium does not adapt to and mortality.
Brief description
Fig. 1 is the Gram’s staining of microexamination yic～bm1 bacterial strain and assembles agglomerating phenomenon figure.
Yic～bm1 bacterial strain phylogenetic tree schematic diagram based on 16srdna sequence construct for the Fig. 2.
Fig. 3 is the schematic diagram of the impact to yic～bm1 strain enzyme-producing for the different temperatures.
Fig. 4 is the impact schematic diagram to yic～bm1 strain enzyme-producing for the different ph.
Specific embodiment
Further illustrate the present invention with reference to instantiation.
Embodiment 1
Fresh sargassum thunbergii after clean drying is worn into 50～60 mesh powder；
Marine algae powder loads in fermentation tank with water according to mass ratio 2:100, and sterilize under the conditions of 115 DEG C 35min, is produced
Product a；And prepare seed liquor: bacillus megaterium yic～bm1 is seeded to fluid medium and cultivates 20 hours；Described huge bud
The preserving number of spore bacillus yic～bm1 is cgmcc no.12156；Described fluid medium is: sodium alginate 5g, ammonium sulfate 5g,
Magnesium sulfate 1g, the mixture of dipotassium hydrogen phosphate 2g, ferrous sulfate 0.01g and distilled water 1000ml, ph7.2～7.4；Huge spore
The volume ratio of bacillus yic～bm1 and culture medium is 2:100.Seed liquor is added in product a, wherein seed liquor and product a
Mass ratio be 1:100, obtain product b；It is 20 DEG C in temperature, under conditions of ph5.5～6.0, product b is fermented, fermentation
Time is 7 days, obtains product c；The ph to 5.0～6.0 of adjustment product c, and temperature is adjusted with per hour 20 DEG C of temperature change
To 5 DEG C, preserve 24h, obtain product d；The temperature of product d is adjusted to 20 DEG C with per hour 20 DEG C of temperature change, ph to 5.5～
6.0, then supplement seed liquor, the seed liquor supplemented is 1:100 with the mass ratio of product d, preserves 24h, obtains product e；Adjustment
The ph of product e to 5.0～6.0, and with per hour 20 DEG C of temperature change adjustment temperature to 5 DEG C, preserve 24h, obtain product f；
The temperature of product f is adjusted to 20 DEG C with per hour 20 DEG C of temperature change, ph to 5.5～6.0, then supplements seed
Liquid, the seed liquor supplemented is 1:100 with the mass ratio of product f, preserves 24h, obtains product g；Huge by obtaining after product g filter pressing
Bacillus cereuss yic～bm1 filtrate；By water, nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, Organic substance containing humic acidss and bacillus megaterium yic～
Alg3 filtrate is added to according to mass ratio 50:22:8:8:6:6 after dissolving completely in stirred tank, obtains containing bacillus megaterium
Liquid Water soluble fertilizer with humic acidss.
Embodiment 2
Fresh Sargassum phyllocystum Tseng et Lu,Sargassum horneri (Turn.) C. Ag. (Fucus horneri (Turn.)C.Ag.,Spongocarpus horneri Kutz.) after clean drying and new fresh sargassum are compounded according to mass ratio 1:1, wears into 50～60 mesh powder；
Marine algae powder loads in fermentation tank with water according to mass ratio 6:100, and sterilize under the conditions of 115 DEG C 25min, is produced
Product a；And prepare seed liquor: the bacillus megaterium yic～bm1 of cellulase-producing is seeded to fluid medium, trains at 35 DEG C
Support 24 hours；The preserving number of the bacillus megaterium yic～bm1 of described cellulase-producing is cgmcc no.12156；Produce fiber
The volume ratio of the bacillus megaterium yic～bm1 of plain enzyme and culture medium is 5:100.Seed liquor is added in product a, its
Middle seed liquor is 10:100 with the mass ratio of product a, obtains product b；It is 35 DEG C in temperature, to product under conditions of ph6.0～6.5
Product b is fermented, and fermentation time is 8 days, obtains product c；The ph to 5.5～6.0 of adjustment product c, and with per hour 3 DEG C of temperature
Degree change adjustment temperature, to 5 DEG C, preserves 12h, obtains product d；With the temperature of per hour 3 DEG C of temperature change adjustment product d extremely
35 DEG C, ph to 6.0～6.5, then supplement seed liquor, the seed liquor supplemented is 6:100 with the mass ratio of product d, preserves 12h,
Obtain product e；The ph to 5.5～6.0 of adjustment product e, and with per hour 3 DEG C of temperature change adjustment temperature to 5 DEG C, preserve
12h, obtains product f；The temperature of product f is adjusted to 35 DEG C with per hour 3 DEG C of temperature change, ph to 6.0～6.5, then mends
Fill seed liquor, the seed liquor supplemented is 6:100 with the mass ratio of product f, preserves 12h, obtains product g；To obtain after product g filter pressing
To bacillus megaterium yic～bm1 filtrate；By water, nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, Organic substance containing humic acidss and bacillus megaterium
Yic～bm1 filtrate is added to according to mass ratio 50:22:8:8:6:6 after dissolving completely in stirred tank, obtains containing huge spore
The liquid Water soluble fertilizer of bacillus and humic acidss.
Embodiment 3
New fresh sargassum after clean drying is worn into 50～60 mesh powder；
Marine algae powder loads in fermentation tank with water according to mass ratio 6:100, and sterilize under the conditions of 125 DEG C 35min, is produced
Product a；And prepare seed liquor: bacillus megaterium yic～bm1 is seeded to fluid medium and cultivates 24 hours；Described huge bud
The preserving number of spore bacillus yic～bm1 is cgmcc no.12156；Described fluid medium is: sodium alginate 5g, ammonium sulfate 5g,
Magnesium sulfate 1g, the mixture of dipotassium hydrogen phosphate 2g, ferrous sulfate 0.01g and distilled water 1000ml, ph7.2～7.4；Huge spore
The volume ratio of bacillus yic～bm1 and culture medium is 8:100.Seed liquor is added in product a, wherein seed liquor and product a
Mass ratio be 4:100, obtain product b；It is 30 DEG C in temperature, under conditions of ph7～7.5, product b is fermented, during fermentation
Between be 9 days, obtain product c；The ph to 5.0～6.0 of adjustment product c, and with per hour 1 DEG C of temperature change adjustment temperature to 5
DEG C, preserve 18h, obtain product d；The temperature of product d is adjusted to 30 DEG C with per hour 8 DEG C of temperature change, ph to 7～7.5, so
Supplement seed liquor afterwards, the seed liquor supplemented is 4:100 with the mass ratio of product d, preserves 18h, obtains product e；Adjustment product e's
Ph to 5.0～6.0, and with per hour 1 DEG C of temperature change adjustment temperature to 5 DEG C, preserve 18h, obtain product f；
The temperature of product f is adjusted to 30 DEG C with per hour 8 DEG C of temperature change, ph to 7.0～7.5, then supplements seed
Liquid, the seed liquor supplemented is 4:100 with the mass ratio of product f, preserves 18h, obtains product g；Huge by obtaining after product g filter pressing
Bacillus cereuss yic～bm1 filtrate；By water, nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, Organic substance containing humic acidss and bacillus megaterium yic～bm1
Filtrate is added to according to mass ratio 50:22:8:8:6:6 after dissolving completely in stirred tank, obtains containing bacillus megaterium and corruption
The liquid Water soluble fertilizer of phytic acid.
Embodiment 4
New fresh sargassum after clean drying is worn into 50～60 mesh powder；
Marine algae powder loads in fermentation tank with water according to mass ratio 9:100, and sterilize under the conditions of 140 DEG C 15min, is produced
Product a；And prepare seed liquor: bacillus megaterium yic～bm1 is seeded to fluid medium and cultivates 20 hours；Described huge bud
The preserving number of spore bacillus yic～bm1 is cgmcc no.12156；Described fluid medium is: sodium alginate 5g, ammonium sulfate 5g,
Magnesium sulfate 1g, the mixture of dipotassium hydrogen phosphate 2g, ferrous sulfate 0.01g and distilled water 1000ml, ph7.2～7.4；Huge spore
The volume ratio of bacillus yic～bm1 and culture medium is 5:100.Seed liquor is added in product a, wherein seed liquor and product a
Mass ratio be 8:100, obtain product b；It is 35 DEG C in temperature, under conditions of ph6.5～7.0, product b is fermented, fermentation
Time obtained product c for 9 days；The ph to 5.0～6.0 of adjustment product c, and with per hour 3 DEG C of temperature change adjustment temperature to 5
DEG C, preserve 12h, obtain product d；The temperature of product d is adjusted to 35 DEG C with per hour 3 DEG C of temperature change, ph to 6.5～7.0,
Then supplement seed liquor, the seed liquor supplemented is 8:100 with the mass ratio of product d, preserves 12h, obtains product e；Adjustment product e
Ph to 5.0～6.0, and with per hour 3 DEG C of temperature change adjustment temperature to 5 DEG C, preserve 12h, obtain product f；
The temperature of product f is adjusted to 35 DEG C with per hour 3 DEG C of temperature change, ph to 6.5～7.0, then supplements seed
Liquid, the seed liquor supplemented is 8:100 with the mass ratio of product f, preserves 12h, obtains product g；Huge by obtaining after product g filter pressing
Bacillus cereuss yic～bm1 filtrate；By water, nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, Organic substance containing humic acidss and bacillus megaterium yic～bm1
Filtrate is added to according to mass ratio 50:22:8:8:6:6 after dissolving completely in stirred tank, obtains containing bacillus megaterium and corruption
The liquid Water soluble fertilizer of phytic acid.
Comparative example 1
Do not adjust the temperature in sweat and ph value, keep the original temperature of environment and the ph value of material itself, other
Condition and material proportion consistent with embodiment 2, operating procedure is with embodiment 2.
Comparative example 2
Obstructed everfermentation, without microbial inoculum, substitutes microbial bacterial agent with Sargassum extract, by water, nitrogenous fertilizer, phosphate fertilizer, potash fertilizer,
Organic substance containing humic acidss and solubility Sargassum extract are added in stirred tank according to mass ratio 50:22:8:8:6:6 and dissolve completely
Afterwards, obtain the seaweed liquid Water soluble fertilizer of humic acidss.
Embodiment 1 to 4, in comparative example 1 to 2, described nitrogenous fertilizer is carbamide, and phosphate fertilizer is industrial grade monoammonium phosphate, and potash fertilizer is sulfur
Sour potassium, Organic substance containing humic acidss is potassium humate.Described Sargassum extract is obstructed everfermentation technique, is dissolved by immersion
The soluble organic fraction that mode is extracted from Sargassum.
Product checking: according to People's Republic of China (PRC) agricultural industry criteria ny/t 798-2015, People's Republic of China's agriculture
Industry industry standard ny/t 1106-2010 detects, testing result is shown in Table 1 to product indices.
Table 1: the quality measurements of the embodiment of the present invention and comparative example
Note: effectively viable bacteria refers to the bacillus megaterium of present invention employing.
Result above shows, the microbial manure indices that the present invention produces are obviously higher than national standard, Er Qiejing
Everfermentation is processed, and in fertilizer, the content of heavy metal substantially reduces, and miscellaneous bacteria rate substantially reduces, and the fermentation technology that the present invention adopts is permissible
Living bacteria count in product is greatly improved, with the obvious advantage in like product.
Embodiments of the invention and comparative example related fertilizer are imposed on pepper planting plot, mu consumption is 40kg.Planting season
For 1 year, soil property specific performance index was shown in Table 2.
Table 2: the property indices after soil improvement
Note: effectively viable bacteria refers to the bacillus megaterium of present invention employing.
Result shows: the present invention adopt strain survive in soil and grows, soil function be improved significantly, ventilate
Porosity is 12.9%-14.6%, soil ph 6.4-6.6, soil weight 1.38-1.41g/cm3, content of beary metal effectively drops
Low.
Apply the embodiment and comparative example product impact situation to Pepper crops in the present invention, with the bacterium applying market purchase
Fertile class product contrast, fertilizer efficiency the results are shown in Table 4.
Table 4: fertilizer efficiency comparing result
Result shows: in the case that dose is suitable, the microbiological compound fertilizer product using the present invention is made hence it is evident that being lifted
Article matter, greatly reduces effect pest and disease damage, effectively strengthens the resistance of crop, can raising effect yield 10.1%-10.9%.
The preserving number of described bacillus megaterium yic～bm1 is cgmcc no.12156；Depositary institution's title is: China
Microbiological Culture Collection administration committee common micro-organisms center；Depositary institution address is: BeiChen West Road, Chaoyang District, BeiJing City 1
Institute 3；Preservation date: on March 2nd, 2016；Latin Classification And Nomenclature is: bacillus megaterium.
The biological nature of described bacillus megaterium yic～bm1 is as follows:
Cellulose culture medium: Carboxymethyl cellulose sodium 15g, ammonium nitrate 1g, magnesium sulfate 1g, potassium dihydrogen phosphate 2g, agar
20g, distilled water 1000ml.
Liquid fermentation medium: Carboxymethyl cellulose sodium 10g, peptone 3g, yeast powder 0.5g, ammonium nitrate 2g, magnesium sulfate
0.3g, potassium dihydrogen phosphate 2g, calcium chloride 0.3g, distilled water 1000ml, ph7.0-7.4.
After yic～bm1 bacterial strain grows 48h on cellulose culture medium flat plate, bacterium colony is circular, white, surfacing.Micro-
Sem observation bacterial strain is Gram-positive, shaft-like, can move, and produces spore.20～40 DEG C of growth temperature range, ph scope 6-10,
Nacl concentration range 0～20%, 37 DEG C of bacterial strain optimum growth temperature, the most suitable growth ph 7.5.Catalase, oxidase, galactoside
Enzyme positive, indole, vp experiment is negative, does not go back orthonitric acid；Can utilize glucose, mannose, Lactose, Raffinose, sucrose, I
Uncle's sugar, xylose, Mannitol, Sorbitol.According to its physiological and biochemical property, this identification of strains is bacillus megaterium bacillus
megaterium.
Fig. 1 finds out, yic～bm1 bacterial strain is gram positive bacteria；
Fig. 2 finds out, the sibship of yic～bm1 bacterial strain is nearest with bacillus megaterium；
Fig. 3 finds out, the most suitable producing enzyme temperature of yic～bm1 bacterial strain is 35 DEG C；
Fig. 4 finds out, optimal producing enzyme ph of yic～bm1 bacterial strain is 6.0.
Claims (8)
1. a kind of production method of the liquid Water soluble fertilizer containing bacillus megaterium and humic acidss is it is characterised in that according to following step
Suddenly carry out: by water, nitrogenous fertilizer, phosphate fertilizer, potash fertilizer, Organic substance containing humic acidss and bacillus megaterium filtrate according to mass ratio 30～75:5
～40:5～40:5～40:5～40:1～80 are added in stirred tank, after stirring and dissolving, obtain containing bacillus megaterium and
The liquid Water soluble fertilizer of humic acidss；
Wherein Organic substance containing humic acidss be two kinds of one of potassium humate, sodium humate and potassium fulvate or arbitrary proportion with
On；
Wherein bacillus megaterium filtrate is prepared from according to following steps:
1), prepare marine algae powder: the Sargassum after drying is clayed into power；
2), prepare product a: marine algae powder and water and load in fermentation tank according to mass ratio 1～10:100, in 100～150 DEG C of conditions
Lower sterilizing 15～35min, obtains product a；
And prepare seed liquor: bacillus megaterium yic～bm1 is seeded to fluid medium, in 25～40 degree of cultures 20～30
Hour obtains seed liquor；
Volume ratio between bacillus megaterium and culture medium is 1～10:100；
3), prepare product b: seed liquor is added in product a and obtains product b, wherein seed liquor and the mass ratio of product a are 1
～10:100；
4), prepare product c: be 25 DEG C～40 DEG C in temperature, under conditions of ph5～9, product b fermented, fermentation time is 6
～10 days, obtain product c；
5), prepare product d: the ph to 5.0～6.0 of adjustment product c, temperature, to 3～10 DEG C, preserves 3h～72h, obtains product d；
6), prepare product e: the temperature of adjustment product d to 25 DEG C～40 DEG C, ph to 5～9, then supplements seed liquor, the kind supplemented
Sub- liquid is 1～10:100 with the mass ratio of product d, preserves 3h～72h, obtains product e；
7), prepare product f: the ph to 5.0～6.0 of adjustment product e, temperature, to 3～10 DEG C, preserves 3h～72h, obtains product f；
8), prepare product g: the temperature of adjustment product f to 25 DEG C～40 DEG C, ph to 5～9, then supplements seed liquor, the kind supplemented
Sub- liquid is 1～10:100 with the mass ratio of product f, preserves 3h～72h, obtains product g；
9), prepare bacillus megaterium filtrate: obtain bacillus megaterium filtrate by after product g filter pressing.
2. the production method of the liquid Water soluble fertilizer containing bacillus megaterium and humic acidss as claimed in claim 1, its feature
It is: described fluid medium is made into according to following mass ratio: 5 parts of sodium alginate, 5 parts of ammonium sulfate, 1 part of magnesium sulfate, phosphoric acid hydrogen
2 parts of dipotassium, 0.01 part of ferrous sulfate and 1000 parts of distilled water.
3. the production method of the liquid Water soluble fertilizer containing bacillus megaterium and humic acidss as claimed in claim 1, its feature
It is: step 2) mass ratio of described marine algae powder and water is 4:100；115 DEG C of sterilising temp, sterilization time 25min；Preparation kind
During sub- liquid, bacillus megaterium is seeded in fluid medium, and culture obtains seed liquor, bacillus megaterium and culture in 22 hours
The volume ratio of base is 5:100；In step 5) and step 7), temperature is 5 DEG C, and the holding time is 12h.
4. the production method of the liquid Water soluble fertilizer containing bacillus megaterium and humic acidss as claimed in claim 1, its feature
It is: in step 4), step 6) and step 8), temperature is 25 DEG C, ph is 6.5～7.0；Step 4) fermentation time is 8 days；Step
6) and the step 8) holding time be 12h；Fermentation seed in the mass ratio of fermentation seed liquid and product d and step 8) in step 6)
Liquid is 5:100 with the mass ratio of product f.
5. the production method of the liquid Water soluble fertilizer containing bacillus megaterium and humic acidss as claimed in claim 1, its feature
It is: in step 5), step 6) step 7) and step 8), adjustment temperature is and adjusts 3 DEG C per hour.
6. the production of the liquid Water soluble fertilizer containing bacillus megaterium and humic acidss as described in any one of claim 1-5
Method it is characterised in that: described Sargassum refers to sargassum thunbergii, Thallus Laminariae (Thallus Eckloniae), opotism, one of Sargassum phyllocystum Tseng et Lu,Sargassum horneri (Turn.) C. Ag. (Fucus horneri (Turn.)C.Ag.,Spongocarpus horneri Kutz.) and Alga Sgrgassi Enervess or arbitrary proportion
Two or more.
7. the production of the liquid Water soluble fertilizer containing bacillus megaterium and humic acid as described in any one of claim 1-5
Side
Method it is characterised in that: described bacillus megaterium is bacillus megaterium yic～bm1, preserving number be gmcc
no.12156.
8. the production method of the liquid Water soluble fertilizer containing bacillus megaterium and humic acid as claimed in claim 6, its feature
?
In: described bacillus megaterium is bacillus megaterium yic～bm1, and preserving number is cgmcc no.12156.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610776115.9A CN106348884A (en) | 2016-08-31 | 2016-08-31 | Production method of bacillus megaterium and humic acid containing liquid water-soluble fertilizer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610776115.9A CN106348884A (en) | 2016-08-31 | 2016-08-31 | Production method of bacillus megaterium and humic acid containing liquid water-soluble fertilizer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106348884A true CN106348884A (en) | 2017-01-25 |
Family
ID=57857448
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610776115.9A Pending CN106348884A (en) | 2016-08-31 | 2016-08-31 | Production method of bacillus megaterium and humic acid containing liquid water-soluble fertilizer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106348884A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107935770A (en) * | 2017-11-25 | 2018-04-20 | 贵阳开磷化肥有限公司 | A kind of functional form soil remediation Water soluble fertilizer and preparation method thereof |
| CN108727082A (en) * | 2018-07-10 | 2018-11-02 | 安徽六国化工股份有限公司 | A kind of bacillus bacterium solution stablizes store method and bio-feritlizer |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102372517A (en) * | 2011-08-15 | 2012-03-14 | 上海理工大学 | Preparation method and application method of seaweed industrial solid waste organic fertilizer |
| CN102391042A (en) * | 2011-08-03 | 2012-03-28 | 青岛丰泰海洋生物科技有限公司 | Production method of seaweed biological liquid fertilizer |
| CN104262008A (en) * | 2014-09-30 | 2015-01-07 | 青岛嘉瑞生物技术有限公司 | Process for preparing liquid seaweed fertilizer through enzymolysis and fermentation |
| CN104446767A (en) * | 2014-11-17 | 2015-03-25 | 苏州市新巷农艺科技园 | Method for preparing seaweed-containing special fertilizer for flowers and plants |
| CN104447008A (en) * | 2014-12-05 | 2015-03-25 | 中国科学院烟台海岸带研究所 | Method for preparing liquid seaweed fertilizer |
| CN104692880A (en) * | 2013-12-04 | 2015-06-10 | 青岛根源生物技术集团有限公司 | Method for treating alga through microbial fermentation to produce alga fertilizers |
-
2016
- 2016-08-31 CN CN201610776115.9A patent/CN106348884A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102391042A (en) * | 2011-08-03 | 2012-03-28 | 青岛丰泰海洋生物科技有限公司 | Production method of seaweed biological liquid fertilizer |
| CN102372517A (en) * | 2011-08-15 | 2012-03-14 | 上海理工大学 | Preparation method and application method of seaweed industrial solid waste organic fertilizer |
| CN104692880A (en) * | 2013-12-04 | 2015-06-10 | 青岛根源生物技术集团有限公司 | Method for treating alga through microbial fermentation to produce alga fertilizers |
| CN104262008A (en) * | 2014-09-30 | 2015-01-07 | 青岛嘉瑞生物技术有限公司 | Process for preparing liquid seaweed fertilizer through enzymolysis and fermentation |
| CN104446767A (en) * | 2014-11-17 | 2015-03-25 | 苏州市新巷农艺科技园 | Method for preparing seaweed-containing special fertilizer for flowers and plants |
| CN104447008A (en) * | 2014-12-05 | 2015-03-25 | 中国科学院烟台海岸带研究所 | Method for preparing liquid seaweed fertilizer |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107935770A (en) * | 2017-11-25 | 2018-04-20 | 贵阳开磷化肥有限公司 | A kind of functional form soil remediation Water soluble fertilizer and preparation method thereof |
| CN108727082A (en) * | 2018-07-10 | 2018-11-02 | 安徽六国化工股份有限公司 | A kind of bacillus bacterium solution stablizes store method and bio-feritlizer |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US8778048B2 (en) | Biochemical humic acid product prepared from kitchen waste and the method of preparing the same | |
| US20200224153A1 (en) | Hyperthermophilic aerobic fermentation inoculant prepared by using municipal sewage sludge and its method | |
| CN105936881B (en) | One kind is for alignic thermophilic sugared bacillus and its application method of degrading | |
| CN106278493B (en) | It is classified the method that enzymatic isolation method prepares the seaweed organic fertilizer containing oligosaccharides | |
| CN105964680B (en) | A kind of beach saline land continuous cropping cotton soil ecology renovation agent and the preparation method and application thereof | |
| CN104263679A (en) | High-efficiency phosphate-solubilizing bacteria and application thereof | |
| CN106495827A (en) | The method that alkali carries and classification enzymolysis joint prepare the organic liquid fertilizer of ocean containing oligosaccharide | |
| CN106348887A (en) | Method for preparing amino acid liquid water-soluble fertilizer through compound stepped fermentation of seaweed | |
| CN104673675B (en) | A kind of Green microecological compound fertilizer and preparation method thereof | |
| CN106348873A (en) | Method for preparing microelement liquid water-soluble fertilizer through alga composite graded fermentation | |
| CN107628894A (en) | Composite bacteria agent increase soil fertility and its preparation method and application | |
| CN104611270A (en) | Streptomyces strain and application thereof | |
| CN106348884A (en) | Production method of bacillus megaterium and humic acid containing liquid water-soluble fertilizer | |
| CN104805029B (en) | A kind of preparation method of fertilizer | |
| CN106396865B (en) | The preparation method of ureaformaldehyde slow release fertilizer containing composite bacteria agent | |
| CN115594549A (en) | Microbial organic fertilizer for improving saline-alkali soil plough layer structure and preparation method thereof | |
| CN106365709A (en) | Preparation method of seaweed organic-inorganic fertilizer containing bacillus megatherium fungicide | |
| CN106348867A (en) | Method for preparing medium element liquid water-soluble fertilizer by seaweed combined hierarchical fermentation | |
| CN106187615B (en) | Method for producing water-soluble fertilizer containing glycotropic bacillus and amino acid | |
| CN106365802A (en) | Production method of liquid water-soluble fertilizer containing bacillus megatherium fungicide and amino acid | |
| CN106348885A (en) | Method for preparing organic liquid water-soluble fertilizer through combination of alkaline extraction and fermentation | |
| CN113481111A (en) | Efficient biological straw fermentation inoculant and preparation method thereof | |
| CN106047775A (en) | Stain of bacillus megatherium for producing cellulase and application method thereof | |
| CN106518280A (en) | Production method for special organic seaweed fertilizer for cherry | |
| CN106396907A (en) | Production method of water-soluble fertilizer containing Bacillusmegaterium fungicide and amino acids |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170125 |