CN104673675B - A kind of Green microecological compound fertilizer and preparation method thereof - Google Patents
A kind of Green microecological compound fertilizer and preparation method thereof Download PDFInfo
- Publication number
- CN104673675B CN104673675B CN201310636693.9A CN201310636693A CN104673675B CN 104673675 B CN104673675 B CN 104673675B CN 201310636693 A CN201310636693 A CN 201310636693A CN 104673675 B CN104673675 B CN 104673675B
- Authority
- CN
- China
- Prior art keywords
- parts
- culture
- bacillus
- compound fertilizer
- bacillus subtilis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 239000003337 fertilizer Substances 0.000 title claims abstract description 34
- 150000001875 compounds Chemical class 0.000 title claims abstract description 16
- 238000002360 preparation method Methods 0.000 title abstract description 10
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 25
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 25
- 241000228245 Aspergillus niger Species 0.000 claims abstract description 22
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 17
- 241001513093 Aspergillus awamori Species 0.000 claims abstract description 13
- 240000006024 Lactobacillus plantarum Species 0.000 claims abstract description 11
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims abstract description 11
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims abstract description 11
- 239000000084 colloidal system Substances 0.000 claims abstract description 11
- 229940072205 lactobacillus plantarum Drugs 0.000 claims abstract description 11
- 229910052698 phosphorus Inorganic materials 0.000 claims abstract description 11
- 239000011574 phosphorus Substances 0.000 claims abstract description 11
- 241000193755 Bacillus cereus Species 0.000 claims abstract description 10
- 241000194107 Bacillus megaterium Species 0.000 claims abstract description 8
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 8
- 239000000203 mixture Substances 0.000 claims abstract description 7
- 238000000855 fermentation Methods 0.000 claims description 29
- 230000004151 fermentation Effects 0.000 claims description 29
- 238000001035 drying Methods 0.000 claims description 8
- 239000012530 fluid Substances 0.000 claims description 7
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 4
- 238000003892 spreading Methods 0.000 claims description 3
- 230000007480 spreading Effects 0.000 claims description 3
- 241000726221 Gemma Species 0.000 claims description 2
- 238000001914 filtration Methods 0.000 claims description 2
- 241000228212 Aspergillus Species 0.000 claims 2
- 244000025254 Cannabis sativa Species 0.000 claims 1
- 239000002689 soil Substances 0.000 abstract description 19
- 244000005700 microbiome Species 0.000 abstract description 11
- 230000009286 beneficial effect Effects 0.000 abstract description 9
- 238000004519 manufacturing process Methods 0.000 abstract description 8
- 235000015097 nutrients Nutrition 0.000 abstract description 2
- 239000005416 organic matter Substances 0.000 abstract description 2
- 235000013348 organic food Nutrition 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 28
- 241000894006 Bacteria Species 0.000 description 22
- 108090000790 Enzymes Proteins 0.000 description 20
- 102000004190 Enzymes Human genes 0.000 description 20
- 229940088598 enzyme Drugs 0.000 description 20
- 230000001580 bacterial effect Effects 0.000 description 14
- 238000011218 seed culture Methods 0.000 description 14
- 241000196324 Embryophyta Species 0.000 description 13
- 239000001963 growth medium Substances 0.000 description 13
- 239000002054 inoculum Substances 0.000 description 12
- 238000011534 incubation Methods 0.000 description 11
- 239000000047 product Substances 0.000 description 11
- 238000012216 screening Methods 0.000 description 10
- 238000011068 loading method Methods 0.000 description 9
- 239000002609 medium Substances 0.000 description 9
- 230000000813 microbial effect Effects 0.000 description 9
- 239000007787 solid Substances 0.000 description 9
- 210000003608 fece Anatomy 0.000 description 8
- 239000007788 liquid Substances 0.000 description 8
- KWGKDLIKAYFUFQ-UHFFFAOYSA-M lithium chloride Chemical compound [Li+].[Cl-] KWGKDLIKAYFUFQ-UHFFFAOYSA-M 0.000 description 8
- 239000010871 livestock manure Substances 0.000 description 8
- 239000000463 material Substances 0.000 description 8
- 238000000034 method Methods 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 7
- 238000006243 chemical reaction Methods 0.000 description 7
- 230000001954 sterilising effect Effects 0.000 description 7
- WTLKTXIHIHFSGU-UHFFFAOYSA-N 2-nitrosoguanidine Chemical compound NC(N)=NN=O WTLKTXIHIHFSGU-UHFFFAOYSA-N 0.000 description 6
- 102000004139 alpha-Amylases Human genes 0.000 description 6
- 108090000637 alpha-Amylases Proteins 0.000 description 6
- 239000012141 concentrate Substances 0.000 description 6
- 238000010790 dilution Methods 0.000 description 6
- 239000012895 dilution Substances 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 239000000725 suspension Substances 0.000 description 6
- 108010059892 Cellulase Proteins 0.000 description 5
- 229940106157 cellulase Drugs 0.000 description 5
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 4
- 229920002472 Starch Polymers 0.000 description 4
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 4
- VTYYLEPIZMXCLO-UHFFFAOYSA-L calcium carbonate Substances [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 4
- 229910000019 calcium carbonate Inorganic materials 0.000 description 4
- 230000008859 change Effects 0.000 description 4
- 239000002361 compost Substances 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 230000035772 mutation Effects 0.000 description 4
- 239000000843 powder Substances 0.000 description 4
- 239000011780 sodium chloride Substances 0.000 description 4
- 235000019698 starch Nutrition 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 3
- 239000001888 Peptone Substances 0.000 description 3
- 108010080698 Peptones Proteins 0.000 description 3
- 240000008042 Zea mays Species 0.000 description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 229940024171 alpha-amylase Drugs 0.000 description 3
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 3
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 3
- 235000011130 ammonium sulphate Nutrition 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 230000035558 fertility Effects 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 230000007062 hydrolysis Effects 0.000 description 3
- 238000006460 hydrolysis reaction Methods 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 3
- 235000019796 monopotassium phosphate Nutrition 0.000 description 3
- 235000019319 peptone Nutrition 0.000 description 3
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
- 239000008107 starch Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 238000009423 ventilation Methods 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 229920001353 Dextrin Polymers 0.000 description 2
- 239000004375 Dextrin Substances 0.000 description 2
- 241000209140 Triticum Species 0.000 description 2
- 235000021307 Triticum Nutrition 0.000 description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 210000000481 breast Anatomy 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 235000005822 corn Nutrition 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 235000019425 dextrin Nutrition 0.000 description 2
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 230000033001 locomotion Effects 0.000 description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 2
- 235000019341 magnesium sulphate Nutrition 0.000 description 2
- 230000002503 metabolic effect Effects 0.000 description 2
- 238000002703 mutagenesis Methods 0.000 description 2
- 231100000350 mutagenesis Toxicity 0.000 description 2
- 239000003895 organic fertilizer Substances 0.000 description 2
- 230000000704 physical effect Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000011343 solid material Substances 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 238000012549 training Methods 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- 244000153158 Ammi visnaga Species 0.000 description 1
- 235000010585 Ammi visnaga Nutrition 0.000 description 1
- 240000006439 Aspergillus oryzae Species 0.000 description 1
- 235000002247 Aspergillus oryzae Nutrition 0.000 description 1
- 229930192334 Auxin Natural products 0.000 description 1
- 101710130006 Beta-glucanase Proteins 0.000 description 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- 235000005979 Citrus limon Nutrition 0.000 description 1
- 244000131522 Citrus pyriformis Species 0.000 description 1
- 229920002261 Corn starch Polymers 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- -1 Endo-β-glucanase Proteins 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 229930191978 Gibberellin Natural products 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 229920000715 Mucilage Polymers 0.000 description 1
- 108090000913 Nitrate Reductases Proteins 0.000 description 1
- 102000015439 Phospholipases Human genes 0.000 description 1
- 108010064785 Phospholipases Proteins 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 1
- ZGBSOTLWHZQNLH-UHFFFAOYSA-N [Mg].S(O)(O)(=O)=O Chemical compound [Mg].S(O)(O)(=O)=O ZGBSOTLWHZQNLH-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 241001148470 aerobic bacillus Species 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 description 1
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 239000002363 auxin Substances 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 1
- 108010047754 beta-Glucosidase Proteins 0.000 description 1
- 102000006995 beta-Glucosidase Human genes 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000012267 brine Substances 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 230000009514 concussion Effects 0.000 description 1
- IQFVPQOLBLOTPF-HKXUKFGYSA-L congo red Chemical compound [Na+].[Na+].C1=CC=CC2=C(N)C(/N=N/C3=CC=C(C=C3)C3=CC=C(C=C3)/N=N/C3=C(C4=CC=CC=C4C(=C3)S([O-])(=O)=O)N)=CC(S([O-])(=O)=O)=C21 IQFVPQOLBLOTPF-HKXUKFGYSA-L 0.000 description 1
- 239000008120 corn starch Substances 0.000 description 1
- 229940099112 cornstarch Drugs 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 150000004985 diamines Chemical class 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000013401 experimental design Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 235000013312 flour Nutrition 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 description 1
- 239000003448 gibberellin Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 239000003864 humus Substances 0.000 description 1
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 235000009973 maize Nutrition 0.000 description 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 1
- 229910000357 manganese(II) sulfate Inorganic materials 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 238000005360 mashing Methods 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 239000013028 medium composition Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000011056 performance test Methods 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 108010009004 proteose-peptone Proteins 0.000 description 1
- 238000013341 scale-up Methods 0.000 description 1
- 238000012807 shake-flask culturing Methods 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 238000004513 sizing Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical compound O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 description 1
- 238000010563 solid-state fermentation Methods 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- 235000015099 wheat brans Nutrition 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2101/00—Agricultural use
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Virology (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Mycology (AREA)
- Botany (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Soil Sciences (AREA)
- Materials Engineering (AREA)
- Fertilizers (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of Green microecological compound fertilizer and preparation method thereof, Green microecological compound fertilizer composition is：Aspergillus niger culture, colloid bacillus cereus microbial inoculum, phosphorus decomposing bacillus megaterium microbial inoculum, bacillus subtilis culture, aspergillus awamori culture, Lactobacillus plantarum agent.The Fertilizer nutrient of the present invention is enriched, and the content of organic matter is high.It disclosure satisfy that the production needs of organic food.The fertilizer of the present invention is formed by compound microorganism ferments, can improve the micro-ecological environment of soil, beneficial conditions are provided for the growth of crops.
Description
Technical field
The present invention relates to a kind of Green microecological compound fertilizer, belongs to agricultural fertilizer technical field.
Background technology
Microorganism fertilizer is a kind of to cause crops to obtain the micro- of specific fertilizer effect with microbial life activity and its product
Biological living product, it improves chemical fertilizer utilization ratio in culture fertility, suppresses the harmful substance such as crops heavy metal and agricultural chemicals
Absorb, purification and rehabilitating soil, promote the decomposed utilization of agricultural crop straw and municipal refuse, improving quality of agricultural product etc. has
Irreplaceable effect.The effect of microbial manure, is played mainly by the synergistic effect to traditional fertilizer, organic fertilizer, right
The improvement activation of soil, and the mode such as physiological action of microorganism are realized.Therefore, microbial manure just has change
Fertilizer, organic fertilizer, a variety of fertility and effect of beneficial organism bacterium, it is activated fertilizer nutrient, improves effect of fertilizer, Crop Improvement product
Matter, the preferable fertilizer for promoting agricultural production efficiency.Microbial manure is live body fertilizer, and its effect contains a large amount of mainly by it
Beneficial microorganism vital movement is completed.Only when these beneficial microbes are in vigorous breeding and metabolic situation
Under, material conversion and beneficial metabolic product could be formed constantly.Therefore, beneficial microorganism species, life in microbial manure
It is the basis of its validity that whether activity vigorous, is in the form of the essential elements such as nitrogen, phosphorus, potassium and how many rather than other fertilizer
Based on.Just because of microbial manure is preparation living, so its fertilizer efficiency and the close phase of number of viable, intensity and ambient environmental conditions
Close, including temperature, moisture, acid-base value, nutritional condition and original live in indigenous microorganism repulsive interaction in soil and have one to be fixed
Ring.
Due to China, research lacks input in terms of microbial manure for a long time so that the microbial manure industry in China is still
Have that integral level is not high, technological innovation is insufficient, product quality and application effect show understable problem.It is sustainable in agricultural
Development has turned into today of human consensus, and these problems are into microorganism fertilizer industry letter " bottleneck " to be got through.
The content of the invention
The technical problems to be solved by the invention are overcome the deficiencies in the prior art, there is provided a kind of Green microecological is compound
Fertilizer：
Parts by weight form：Aspergillus niger culture 5-10 parts, colloid bacillus cereus microbial inoculum 3-8 parts, the huge gemma of phosphorus decomposing
Bacillus microbial inoculum 3-8 parts, bacillus subtilis culture 5-12, aspergillus awamori culture 4-7, Lactobacillus plantarum agent 2-4 parts.
Aspergillus niger (Aspergillus niger) Li-2013-03 preserving numbers are CGMCC NO.7927.
It is prepared by the bacillus subtilis culture：Transferred from inclined-plane and cultivate bacillus subtilis, the kind after spreading cultivation step by step
Sub- liquid is transferred in fermentation tank, and fermentation finishes zymotic fluid and obtains bacillus subtilis culture after plate-frame filtering, drying.
The preparation method of Lactobacillus plantarum agent：Transferred from inclined-plane and cultivate Lactobacillus plantarum, the seed liquor after spreading cultivation step by step turns
Access in fermentation tank, fermentation finishes zymotic fluid is concentrated in vacuo to original volume through low-temperature negative-pressure 45%, obtains bacterium concentrate.Addition
Carrier：The carrier mixed is added into concentrate, is well mixed；The weight of concentrate and carrier ratio is 0.5-0.6：1, carrier
Form and be：CaCO320-30 parts, dextrin 10-15 parts.Fluidized bed drying, 50 DEG C of drying temperature.
It is prepared by aspergillus awamori culture：Spawn incubation, solid fermentation culture：Spore liquid is inoculated into the training of aspergillus oryzae solid state fermentation
In nutriment, 26-33 DEG C of culture covers with compost to mycelia, and low temperature fluidized bed dry, pulverize dried object.
It is prepared by aspergillus niger culture：Spawn incubation, prepared using conventional solid fermentation culture method：Spore liquid is inoculated into solid
In state fermented and cultured material, 26-33 DEG C of culture covers with compost to mycelia, dry, pulverize dried object.
Beneficial effect
Baoding Rui Gu bio tech ltd provides colloid bacillus cereus bacterium powder；
The fertilizer of the present invention can strengthen the drought-resistant ability of crop.Colloid bacillus cereus energy in the composite bacteria of the present invention
Secrete the growth promoting substance such as auxin material and gibberellin material, extraneous root strong sprout；Decompose element silicon in soil to utilize for plant, make
The wax coat for obtaining plant thickens, and improves plant water keeping ability；Soil granular structure can be promoted to be formed, kept soil from packing together；Destroy
Soil capillarity, prevents soil water evaporation.
The fertilizer of the present invention is formed by compound microorganism ferments, can improve the micro-ecological environment of soil, is crops
Growth provides beneficial conditions.The fertilizer of the present invention being capable of improved soil.Beneficial microbe can produce glucide in fertilizer, account for soil
The 0.1% of earth organic matter, with plant mucilage, mineral idiosome and organic colloid are combined together, and can improve soil granular structure,
Strengthen the physical property of soil and reduce the loss of soil particle, under certain conditions, moreover it is possible to participate in humus and formed.So
It can improve soil physical property using microbial manure, be advantageous to increase soil fertility.
This product application method is simple, and every mu of 0.3-1 kilograms of usage amount in ground, cost is only 80-100 members/mu, seed dressing or raw
Earth's surface is sprayed before pouring water for a long time.
Embodiment
The present invention is described below by specific embodiment.Unless stated otherwise, technological means used in the present invention
It is method known in those skilled in the art.In addition, embodiment is interpreted as illustrative, it is not intended to limit the present invention
Scope, the spirit and scope of the invention are limited only by the claims that follow.To those skilled in the art, without departing substantially from this
The various changes carried out on the premise of invention spirit and scope to the material component in these embodiments and dosage or change
Belong to protection scope of the present invention.
Aspergillus niger strain (Aspergillus niger) Li-2013-03 provided by the invention is by the one of Laboratories Accession
The more wheel nitrosoguanidine mutagenesis of aspergillus niger (Aspergillus niger) Li-2010 warps of strain cellulase-producing production, then to mutation
Strain step-sizing is eliminated, and finally the fermented performance test of strain excellent is screened to obtain the black-koji mould for producing high activity cellulase
Strain (Aspergillus niger) Li-2013-03.
The bacterial strain of the high activity cellulase of production provided by the invention is specially aspergillus niger (Aspergillus niger) Li-
2013-03.The bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms on July 15th, 2013
(abbreviation CGMCC, address are the heart：City of BeiJing, China Chaoyang District North Star West Road 1 institute 3, postcode 100101), protecting a surname number is
CGMCCNO.7927。
The screening technique of high activity cellulase bacterial strain is produced, is comprised the following steps：
1) inclined-plane culture：By original Aspergillus niger strain (Aspergillus niger) Li-2010 streak inoculation inclined-plane cultures
Base, 30 DEG C of 2~3d of culture, until mycelia body maturation, a large amount of black spores of production.The slant medium composition is as follows：12OBrix
Brewer's wort 1000mL, pH value is naturally, 121 DEG C of sterilizing 20min；
2) spore suspension is prepared (following steps aseptically operate)：15mL sterilized waters are added to test tube slant,
Spore is scraped, filtered with filter paper, filtered solution is poured into and sterilized and added with the 150mL triangular flasks of 5-10 grain sterile glass beads
In, triangular flask is put into shaking table vibration 10-15min, disperses spore.
3) nitrosoguanidine (NTG) mutagenesis
A. spore suspension is adjusted to sterilized water be diluted to 106-107Individual/mL.
B. take 10mL bacteria suspensions to be transferred in 100mL triangular flasks, add 10mg NTG, be configured to final concentration of 10mg/mL
NTG solution, and 4-5 drop acetone is added, so that NTG dissolves.
C. the 200rpm oscillating reactions 30min at 30 DEG C, 5000rpm centrifugation 10min collect thalline, use sterile saline
Shen Di for several times, stopped reaction.
D. suitably dilute and spore concentration is adjusted to 103Individual/mL, takes the bacterium solution 0.2mL of last dilution factor, dilution spread in
On cellulose-Congo red plate screening culture medium.Picking transparent circle/larger bacterial strain of colony diameter after being cultivated 2~3 days at 30 DEG C
200.(cellulose-Congo red plate screening culture medium composition is as follows：Cellulose powder 10g, Congo red 0.2g, ammonium sulfate
5g, magnesium sulfate 0.25g, potassium dihydrogen phosphate 1g, sodium chloride 0.1g, gelatin 2g, agar 20g, running water constant volume 1000mL, pH value 5-
6,121 DEG C of sterilizing 20min).
E. secondary screening：200 plants of bacterium of acquisition are inoculated in slant medium with sterile toothpick respectively, 30 DEG C of cultures to spore are spread
Full inclined-plane.Spore is fermented with being inoculated under sterile washing in the 250mL triangular flasks equipped with 50mL secondary screening culture mediums respectively,
Inoculum concentration 10% (v/v), 30 DEG C, 100r/min culture 96h, determines the cellulase activity of each bacterial strain respectively.(the secondary screening training
It is as follows to support base composition：Cellulose powder 50g, ammonium sulfate 5g, magnesium sulfate 0.25g, potassium dihydrogen phosphate 1g, sodium chloride 0.1g, running water
Constant volume 1000mL, pH value 5-6,121 DEG C of sterilizing 20min).Choose cellulose enzyme vigor highest bacterial strain and be amplified experiment.
4) genetic stability is tested
Continuous ten passages on inclined-plane by Li-2013-03 bacterial strains, and with after each passage of method detection of shaking flask secondary screening
Fermentation situation.Experiment finds that continuous ten passages, the strain character do not have significant change, property indices on inclined-plane
It is all normal, illustrate that the genetic stability of the strain is stronger.
5) scale-up
1. seed culture：Cellulose enzyme vigor highest strains A spergillus nigerLi-2013-03 are accessed
In 500mL triangular flasks, 100 milliliters of seed culture medium loading amount, 30 DEG C, 150rpm shaking table cultures 72-96h.
3. seed tank culture：By seed liquor with 10L fermentation tank of 10% (v/v) inoculum concentration access equipped with 7.5L zymotic fluids
In, constant control ph is 6.0 ± 0.2,30 ± 0.1 DEG C, mixing speed 300rpm of cultivation temperature, ventilation (v/v) 1：0.8-
1.2, incubation time 96h, dissolved oxygen 20-30%.The fermentation medium forms：Cellulose powder 100g, ammonium sulfate 5g, sulfuric acid
Magnesium 0.25g, potassium dihydrogen phosphate 1g, sodium chloride 0.1g, running water constant volume 1000mL, pH value 5-6,121 DEG C of sterilizing 20min.
After fermentation ends, fermented supernatant fluid (crude enzyme liquid) is taken to carry out enzyme activity detection after measured, strains A spergillus
Niger Li-2013-03 circumscribed 1,4 beta-glucanase, Endo-β-glucanase, beta-glucosidase and filter paper enzyme activity difference
Reach 620U/mL, 1289U/mL, 456U/mL and 732U/mL, respectively than starting strain Aspergillus niger Li-2010
Improve 9.21 times, 7.43 times, 8.15 times and 10.31 times.
Phosphorus decomposing bacillus megaterium is a kind in bacillus (Bacillus).Motion, pod membrane is formed, it is aerobic.
Acid is produced from glucose, also often from arabinose and mannitol production acid.Hydrolysis starch, does not produce lecithinase, and VP is negative.It can decompose
Organophosphor in soil turns into the available rapid available phosphorus of plant.
The agent of phosphorus decomposing bacillus megaterium is provided by the prosperous hair biotechnology research institute in Cangzhou, address：Transport Hebei China Cangzhou City
River reach liberation West Road is nourished and the area 807-812 of international business affairs center Building A 1.
Baoding Rui Gu bio tech ltd provides colloid bacillus cereus bacterium powder.
Bacillus subtilis (Bacillus subtilis) Li-2013-02 provided by the invention.The bacterial strain is in 2013
On July 15, in is preserved in Chinese microorganism strain Bao Zang administration committees common micro-organisms center, and (abbreviation CGMCC, address are：In
State's Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3), preserving number is CGMCC No.7926.
The bacterial strain feature is as follows：
Bacterial strain colony colour on solid plate is milky, and dry tack free is opaque, neat in edge, for fortune
The aerobic bacteria of dynamic property.Microscopy is elongated rod shape, and Gram's staining is positive.The bacterium can utilize citrate, and nitrate reductase, V-P are real
Test into the positive.
Bacillus subtilis (Bacillus subtilis) Li-2013-02 is by one plant of production for being preserved in this laboratory
The bacillus subtilis Li-2013 of Thermostable α-Amylase obtains through UV-LiCl-dithyl sulfate Mutation screening
, specific screening step is as follows：
(1) preparation of bacteria suspension
By in the Li-2013 single bacterium colonies access seed culture medium grown after plate streaking separates, 100r/min, 40 DEG C are trained
After supporting 12h, take after 1mL medium centrifugals with brine twice, and be resuspended with 9mL physiological saline.
(2) UV-LiCl-dithyl sulfate complex mutation
Bacteria suspension is placed in aseptic flat board, is 30cm in distance, irradiation 100s is stirred under power 15w uviol lamp.Will
Bacterium solution by irradiation is coated on lithium chloride flat board after gradient dilution, and applies flat board with the bacterium solution dilution without ultraviolet irradiation and do
Control.By the uniform flat board of above-mentioned coating, wrapped with the cloth or newspaper of black, put 40 DEG C of culture 48h, growing the flat board of bacterium colony
On filter out hydrolysis circle with colony diameter ratio the maximum choose to inclined-plane preserve, bacteria suspension is configured to after purification, through gradient dilution
It is sufficiently mixed with dithyl sulfate stoste, and in 40 DEG C of concussion processing 40min, treated bacterium solution is applied after gradient dilution afterwards
It is distributed in lithium chloride flat board.
(3) primary dcreening operation of high-yield strains
By the uniform flat board of above-mentioned coating, 40 DEG C of culture 48h are put, primary dcreening operation goes out hydrolysis circle and bacterium on the flat board for grow bacterium colony
Fall diameter ratio the greater to choose to inclined-plane preservation, obtain three plants of bacterium Li-2013-01, Li-2013-02, Li-2013-03 after purification
(4) shake flask fermentation secondary screening
Three plants of bacterium Li-2013-01, Li-2013-02, Li-2013-03 of acquisition are being contained into 30mL fermentation mediums
Carry out shake flask fermentation in 250mL shaking flasks, seed inoculum concentration 10% (V/V), 40 DEG C, 100r/min culture 72h, in centrifuging and taking fermentation
Crude enzyme liquid is made in clear liquid.
(5) enzyme activity determination
The definition of enzyme-activity unit：1mL crude enzyme liquids, under the conditions of 105 DEG C, pH4.2,1min liquefaction 1mg soluble starches, i.e.,
For 1 enzyme activity unit, represented with U/mL.
After measured, bacterial strain Li-2013-02, for stable most superior strain, and enzyme activity reaches 30000U/mL, compares original bacteria
Strain enzyme activity improves 1.6 times.
The lithium chloride flat board：Starch 1%, peptone 1%, (NH)2SO40.4%, K2HPO40.8%, CaCl20.2%,
Lithium chloride 0.9%, agar 2%.
Described seed culture medium：Dusty yeast 0.5%, peptone 1%, soluble starch 1%, NaCl1%.
Described fermentation medium：Corn flour 5%~15%, beancake powder 4%~10%, (NH) 2SO40.4%,
K2HPO40.8%, CaCl20.2%.
Described shake flask culture conditions：The bacterium is in the 250mL shaking flasks containing 30mL fermentation mediums, inoculum concentration 10%
(V/V), 100r/min, 40 DEG C of fermented and cultured 72h.
A kind of high-temperature resistant alpha-amylase is obtained by bacterial strain Li-2013-02 fermentations, its zymologic property is as follows：
(1) the enzyme Acclimation temperature wider range, optimum temperature preserve between 105-115 DEG C below 110 DEG C
Temperature stability it is preferable, and more than 115 DEG C preserve long-time temperature stabilities it is poor.
(2) the enzyme optimal reaction pH value is 4.2.There is high enzyme vigor between pH value 3.0-7.0, be 3.0 in pH value
When enzyme activity complete stability.
(3) enzymatic activity：By mutant strain Li-2013-02 provided by the present invention, the Thermostable α-Amylase enzyme activity of preparation
For 30000-35000U/ml.
1st, the present invention obtains a plant height using the method for UV-LiCl-dithyl sulfate complex mutation and produces resistance to height
The characteristics of bacillus subtilis Li-2013-02 of warm alpha-amylase, the bacterial strain have strong acidproof, heat resistance, and producing enzyme vigor is high.
2nd, the Thermostable α-Amylase enzyme activity for having bacterial strain production gained is up to 30000-35000u/ml,；Applicable temperature
Scope is 25-115 DEG C, 110 DEG C of optimal reactive temperature, in 110 DEG C of enzyme activity complete stabilities；It is 3.0- to be applicable pH value in reaction scope
7.0, the enzyme activity complete stability when pH value is 3.0, optimal reaction pH value 4.2, than existing Thermostable α-Amylase enzyme activity
Height, enzyme effect optimum pH scope is wide in range, and resistance to temperature is high, is particularly suitable for high reaction temperature, liquefaction process and Mashing process and deposits
Industrialization demand.
Embodiment 1
A kind of Green microecological compound fertilizer is provided：Parts by weight form：6 parts of aspergillus niger culture, colloid bacillus cereus
5 parts of microbial inoculum, 5 parts of phosphorus decomposing bacillus megaterium microbial inoculum, bacillus subtilis culture 10, aspergillus awamori culture 6, plant breast bar
3 parts of microbial inoculum.
The strain of use is as follows：
Bacillus subtilis (Bacillus subtilis subsp) CGMCC7926
Lactobacillus plantarum (Lactobacillus plantarum) CICC20764
Aspergillus awamori (Aspergillus awamori) CGMCC3.6484
Aspergillus niger (Aspergillus niger) Li-2013-03 preserving numbers are CGMCC NO.7927.
The preparation method of aspergillus awamori microbial inoculum：
Technical scheme is as follows：
Slant strains activation culture：Aspergillus awamori slant strains are transferred on slant medium, 27 DEG C are cultivated 3 days.
Solid first order seed culture：500 milliliter triangles of the picking aspergillus awamori slant strains access equipped with 100 grams of culture mediums
Seed culture is carried out in bottle, 30 DEG C are cultivated 3 days.
Solid secondary seed culture：Above-mentioned cultured solid first order seed stirring is equipped with 1000 grams to be added after fragment
Seed culture, condition of culture are carried out in 5000 milliliters of triangular flasks of culture medium：30 DEG C are cultivated 3 days.
Solid fermentation culture：Second-level shake flask seed is crushed, adds in fermentation vat or pallet equipped with sterilising medium and mixes
Cultivated after closing uniformly, material cultivation temperature is controlled at 26-35 DEG C, humidity 80-90%, during culture every 10 hours stirrings once
Between 5-7 days；The culture of solid material is using conventional material culture technique；Cultivate, cultivate to the end of compost covers with mycelia
Base is in advance through thermophilic digestion sterilization treatment, sterilising conditions 121 DEG C of temperature of control, 1 hour time.
Drying and crushing：Fermentation ends compost is dried on fluid bed or other drying equipments, drying temperature control
At 60 DEG C, be dried to moisture below 10%, then crushed solid culture medium, crushing material aperture 60 mesh with
On.
Culture medium forms：Solid material：Wheat bran 80%, beancake powder 10%, cornstarch 10%, add equivalent running water；
Initial pH is natural.
The preparation method of bacillus subtilis culture：
1. the acquisition of zymotic fluid：Spread cultivation step by step using slant strains and obtain bacillus subtilis fermentation liquor；
(1) first order seed culture：Bacillus subtilis slant strains are accessed in 500 milliliters of shaking flasks, culture medium loading amount 100
Milliliter, 180 revs/min of rotary shaker, 30 DEG C of cultivation temperature, incubation time 24 hours；
(2) secondary seed culture：First order seed is accessed in 500 milliliters of secondary seed shaking flasks according to 10% inoculum concentration,
Condition of culture is identical with first order seed；
(3) three-level seed culture：Secondary seed is accessed in 5000 milliliters of three-level seed flasks with 10% inoculum concentration, culture
1000 milliliters of base loading amount, 100 revs/min of rotary shaker, 30 DEG C of cultivation temperature, incubation time 24 hours；
(4) first class seed pot culture：Three-level seed is accessed into first class seed pot of the total measurement (volume) as 150L using 10% inoculum concentration,
Fermentation medium loading amount 100L, 28 DEG C of cultivation temperature, 100 revs/min of mixing speed, ventilation (V/V) 1：0.5, tank pressure
0.05Mpa, incubation time 24 hours；
(5) fermented and cultured：First class seed pot strain is accessed into total measurement (volume) as 1.5 tons of secondary seed tanks using 10% inoculum concentration,
1 ton of fermentation medium loading amount, 28 DEG C of condition of culture cultivation temperature, 100 revs/min of mixing speed, ventilation (V/V) 1：0.5, tank pressure
0.05Mpa, incubation time 24 hours.
Culture medium forms：Glucose 6%, yeast extract 1%, peptone 0.2%, CaCO31%, pH6.8.
The preparation method of Lactobacillus plantarum agent：
(1) first order seed culture：Lactobacillus plantarum strain is accessed in 500 milliliters of shaking flasks, 100 milliliters of culture medium loading amount,
30 DEG C of cultivation temperature, incubation time 24 hours；
(2) secondary seed culture：First order seed is accessed in 500 milliliters of secondary seed shaking flasks according to 10% inoculum concentration,
Condition of culture is identical with first order seed；
(3) three-level seed culture：Secondary seed is accessed in 5000 milliliters of three-level seed flasks with 10% inoculum concentration, culture
1000 milliliters of base loading amount, 30 DEG C of cultivation temperature, incubation time 24 hours；
(4) first class seed pot culture：Three-level seed is accessed into first class seed pot of the total measurement (volume) as 150L using 5% inoculum concentration,
Fermentation medium loading amount 100L, 30 DEG C of cultivation temperature, tank pressure 0.05Mpa, incubation time 18 hours；
(5) fermentation tank culture：First class seed pot strain is accessed into total measurement (volume) as 3 tons of secondary seed tanks using 5% inoculum concentration, hair
2 tons of ferment culture medium loading amount, 30 DEG C of condition of culture cultivation temperature, tank pressure 0.05Mpa, incubation time 22 hours.Fermentation finishes fermentation
Liquid is concentrated in vacuo to the 45% of original volume through low-temperature negative-pressure, obtains bacterium concentrate.Add carrier：Add and mix into concentrate
Carrier, be well mixed；The weight of concentrate and carrier ratio is 0.5：1, vehicle group turns into：CaCO325 parts, 12 parts of dextrin.Stream
Change bed to dry, 50 DEG C of drying temperature.
Culture medium forms：Casein peptone 1%, beef extract 1%, yeast extract 0.5%, glucose 0.5%, second
Sour sodium 0.5%, lemon acid diamine 0.2%, Tween800.1%, K2HPO40.2%, MgSO4.7H2O0.02%,
MnSO4.H2O0.005%, CaCO32%, pH6.8.
Embodiment 2
Substantially with embodiment 1
A kind of Green microecological compound fertilizer is provided：Parts by weight form：5 parts of aspergillus niger culture, colloid bacillus cereus
7 parts of microbial inoculum, 6 parts of phosphorus decomposing bacillus megaterium microbial inoculum, bacillus subtilis culture 12, aspergillus awamori culture 6, plant breast bar
2 parts of microbial inoculum.
Product effect experiment
Selection experimental field and experimental design：Test in March in 2009 20 days-September 30 days in Yanchi county Ningxia dapple pond
Town Ba Bao villages are carried out.
Experimental plot reaches 10 mu of field maize planting, uses 0.7 kilogram of every mu of product of the present invention, emergence 1 in plantation respectively
0.6 kilogram of invention product was used by loose ground mode in individual month or so, control group uses common fertilizer.
Invention product reaches 650 kilograms using milpa corn yield, and control group reaches 510 kilograms；The plot was in the 2nd year
Plant spring wheat, spring wheat production have reached 400 kilograms, and 20% is improved than control group per unit area yield.And experimental plot soil texture is good
It is good, no bulk and hardened.
Claims (4)
1. a kind of Green microecological compound fertilizer, parts by weight composition are：Aspergillus niger culture 5-10 parts, colloid bacillus cereus microbial inoculum
3-8 parts, phosphorus decomposing bacillus megaterium microbial inoculum 3-8 parts, bacillus subtilis culture 5-12 parts, aspergillus awamori culture 4-7 parts,
Lactobacillus plantarum agent 2-4 parts；Aspergillus niger (Aspergillus niger) preserving number is CGMCC NO.7927, the withered grass gemma
Bacillus (Bacillus subtilis) is CGMCC NO.7926.
2. Green microecological compound fertilizer according to claim 1, prepared by the bacillus subtilis culture：Transferred from inclined-plane
Cultivate bacillus subtilis, the seed liquor after spreading cultivation step by step transfers in fermentation tank, fermentation finish zymotic fluid by plate-frame filtering,
Bacillus subtilis culture is obtained after drying.
3. Green microecological compound fertilizer according to claim 1, the Green microecological compound fertilizer parts by weight composition are：It is black
6 parts of aspergillus culture, 5 parts of colloid bacillus cereus microbial inoculum, 5 parts of phosphorus decomposing bacillus megaterium microbial inoculum, bacillus subtilis culture
10 parts, 6 parts of aspergillus awamori culture, 3 parts of Lactobacillus plantarum agent.
4. Green microecological compound fertilizer according to claim 1, the Green microecological compound fertilizer parts by weight composition are：It is black
5 parts of aspergillus culture, 7 parts of colloid bacillus cereus microbial inoculum, 6 parts of phosphorus decomposing bacillus megaterium microbial inoculum, bacillus subtilis culture
12 parts, 6 parts of aspergillus awamori culture, 2 parts of Lactobacillus plantarum agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310636693.9A CN104673675B (en) | 2013-11-28 | 2013-11-28 | A kind of Green microecological compound fertilizer and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310636693.9A CN104673675B (en) | 2013-11-28 | 2013-11-28 | A kind of Green microecological compound fertilizer and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104673675A CN104673675A (en) | 2015-06-03 |
| CN104673675B true CN104673675B (en) | 2018-01-26 |
Family
ID=53309270
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310636693.9A Expired - Fee Related CN104673675B (en) | 2013-11-28 | 2013-11-28 | A kind of Green microecological compound fertilizer and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104673675B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105234168A (en) * | 2015-11-20 | 2016-01-13 | 福建康德贝生物科技有限公司 | Application of bacillus subtilis |
| CN105924238A (en) * | 2016-04-20 | 2016-09-07 | 义乌市锦钰信息科技有限公司 | Microbial compound fertilizer and preparation method thereof |
| CN108947673A (en) * | 2018-08-14 | 2018-12-07 | 侯希波 | A kind of soil improvement Natural Circulation microbial bacterial agent and preparation method thereof |
| CN111154661B (en) * | 2020-01-16 | 2021-09-21 | 中化农业（临沂）研发中心有限公司 | Complex microbial inoculant and application thereof |
| CN114806893A (en) * | 2022-04-29 | 2022-07-29 | 辽宁锌硒旺生物科技有限公司 | Composite microbial soil conditioner and production method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1140159A (en) * | 1996-04-29 | 1997-01-15 | 刘文治 | Method for producing organic and inorganic mixed microbe fertilizer |
| CN101186879A (en) * | 2007-12-05 | 2008-05-28 | 中国科学院南京土壤研究所 | Agriculture castoff compost ternary microorganism composite microbial inoculum |
| CN102964155A (en) * | 2012-11-09 | 2013-03-13 | 大连施倍得生物技术有限公司 | Method for preparing internal growth nitrogen-fixing bacteria organic fertilizer |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001199782A (en) * | 2000-01-12 | 2001-07-24 | Marutomo:Kk | Method for producing fertilizer and compost having crumb structure |
| JP2008178841A (en) * | 2007-01-26 | 2008-08-07 | Tokiwa Reitou Shokuhin Kk | Method for treating yu (tofu whey) and liquid fertilizer and deodorization liquid produced by this method |
-
2013
- 2013-11-28 CN CN201310636693.9A patent/CN104673675B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1140159A (en) * | 1996-04-29 | 1997-01-15 | 刘文治 | Method for producing organic and inorganic mixed microbe fertilizer |
| CN101186879A (en) * | 2007-12-05 | 2008-05-28 | 中国科学院南京土壤研究所 | Agriculture castoff compost ternary microorganism composite microbial inoculum |
| CN102964155A (en) * | 2012-11-09 | 2013-03-13 | 大连施倍得生物技术有限公司 | Method for preparing internal growth nitrogen-fixing bacteria organic fertilizer |
Non-Patent Citations (2)
| Title |
|---|
| Plant–microbes interactions in enhanced fertilizer-use efficiency;Anthony O et al.;《Appl Microbiol Biotechnol》;20090826;第85卷;第1-12页 * |
| 微生物有机复合肥在温室黄瓜番茄上应用效果研究;张淑莲;《陕西农业科学》;20051231;第3卷;44-45,96 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104673675A (en) | 2015-06-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103739315B (en) | A kind of preparation method of Green microecological compound fertilizer | |
| CN104673675B (en) | A kind of Green microecological compound fertilizer and preparation method thereof | |
| CN104478532A (en) | Efficient micro-ecological bio-fertilizer and preparation method thereof | |
| CN103739321B (en) | A kind of Green microecological compound fertilizer | |
| CN103739319B (en) | A kind of microbial fertilizer | |
| CN103773720B (en) | A kind of preparation method of microbial fertilizer | |
| CN103739317B (en) | A kind of Microecological compound fertilizer | |
| CN103773719B (en) | A kind of preparation method of Microecological compound fertilizer | |
| CN103626531B (en) | Compound fertilizer and preparation method thereof | |
| CN103739322B (en) | A kind of preparation method of composite bio-fertilizer | |
| CN104805029B (en) | A kind of preparation method of fertilizer | |
| CN103773718B (en) | A kind of preparation method of Novel micro-ecological fertilizer | |
| CN103773698B (en) | A kind of preparation method of high-effective microorganism fertilizer | |
| CN103773717B (en) | A kind of high-effective microorganism is fertile | |
| CN103740616B (en) | A kind of composite bio-fertilizer | |
| CN105907397A (en) | Composite microbial fertilizer and preparation of same | |
| CN103739318B (en) | Preparation method of microbial compound fertilizer | |
| CN103739316B (en) | Microbial compound fertilizer | |
| CN104805028A (en) | Micro-ecological fertilizer | |
| CN104311169A (en) | Compound biological fertilizer and preparation method thereof | |
| CN103739320B (en) | A kind of Novel micro-ecological fertilizer | |
| CN103739323B (en) | A kind of preparation method of composite bio-fertilizer | |
| CN103773716B (en) | A kind of high-efficiency composite biofertilizer | |
| CN105924238A (en) | Microbial compound fertilizer and preparation method thereof | |
| CN104671848A (en) | Microbial compound fertilizer and production method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20210414 Address after: 158100 Yindong village, Jidong Town, Jidong County, Jixi City, Heilongjiang Province Patentee after: Heilongjiang Chenguang bio organic fertilizer manufacturing Co.,Ltd. Address before: 300160 Tianjin City Hedong District Forest Road No. 63 Patentee before: TIANJIN POLYTECHNIC University |
|
| TR01 | Transfer of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180126 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |