CN106316576A - Mushroom collagen culture substrate for facilitating hypha growth - Google Patents
Mushroom collagen culture substrate for facilitating hypha growth Download PDFInfo
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- CN106316576A CN106316576A CN201610645691.XA CN201610645691A CN106316576A CN 106316576 A CN106316576 A CN 106316576A CN 201610645691 A CN201610645691 A CN 201610645691A CN 106316576 A CN106316576 A CN 106316576A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
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Abstract
The invention discloses a mushroom collagen culture substrate for facilitating hypha growth, which is prepared from, by weight, 15-18 parts of edible rice vinegar liquid, 2-3 parts of glucose powder, 1-2 parts of calcium carbonate, 42-46 parts of earthworm casts, 13-15 parts of soya residues, 2-4 parts of calcium acetate, 1-2 parts of zinc sulfate, 3-4 parts of ammonium dihydrogen phosphate, 3-5 parts of pyrolusite, 45-47 parts of pig skin scrap material, 2-3 parts of sodium chloride powder, appropriate amount of sodium chloride solution with mass percentage of 3.1%, appropriate amount of acetum at PH2.5, appropriate amount of sodium hydroxide solution, and appropriate amount of water. The mushroom collagen culture substrate has comprehensive nutritional contents, can extend mushroom fruiting peak through the use of edible rice vinegar liquid, and with the advantage that carbon source and calcium ion in materials such as glucose powder and calcium carbonate can be easily absorbed by the hypha, the mushroom collagen culture substrate can improve the richness of the mushroom sporocarp and benefit the growth of the hyphal; meanwhile, the mushroom collagen culture substrate cultivates robust mycelium and sporocarp, strengthens disease resistance, and improves product quality of the mushroom.
Description
Technical field
The present invention relates to fungus growing technique field, particularly relate to a kind of Lentinus Edodes collagen cultivation base promoting mycelial growth
Matter.
Background technology
Lentinus Edodes is commonly called as China mushroom, is that mycophagy medical material is cultivated in a kind of famous using, has long cultivation in China
Historical tradition, the nutritive value abundant because of it and medical value and deeply liked by consumer, become the second adult in the world
The edible fungi of work cultivation, due to the fast development of industry, the raw material of Lentinus Edodes substituting stuff cultivation traditional matrices is the most exhausted, needs actively
Explore the approach substituting substrate.On the other hand, along with the raising of people's living standard, it is desirable to Lentinus Edodes nutritive value and medicinal
It is worth and also can be improved, so needing to provide the mushroom cultivation substrate of a kind of high-quality.
Collagen protein as a kind of biomacromolecule, have natural hypotoxicity, hypoimmunity, bootable mycelial growth with
And the preferable advantage such as biocompatibility, its extracting method is simple simultaneously, and raw material sources extensively and cheap, are discarded by slaughterhouse
Corii Sus domestica can carry out scale extraction, it has a very high potential as the New-type matrix material of cultivating champignon, but Non-crosslinked
The collagen protein of moditied processing often has that degradation rate is too fast, deformation temperature is low, easily shrink deformation and mechanical performance
The shortcomings such as deficiency, need by the cross-linking modified mechanical performance improving collagen protein and anti-degradation capability.
At present, the method that collagen-stabilizedization processes mainly is cross-linked by method physically or chemically.Wherein,
Physical method has vacuum dehydrothermal, ultraviolet and C ray crosslinking etc., and the advantage of these methods is not introduce toxic chemical substance,
Can keep the biocompatibility that collagen is good, but individually use physical crosslinking tend not to obtain homogeneous, preferably crosslinking strong
Degree, therefore Chemical Crosslinking Methods is more widely applied.
Common chemical cross-linking agent has aldehydes, Carbodiimides, bis-epoxy class material and the different hydrochlorate of cyanogen etc..Wherein,
Glutaraldehyde is the cross-linking agent that a class is widely used in collagen base biological material, can reduce the antigenicity of collagen, but glutaraldehyde is handed over
There is the reduction collagenous biological compatibility and be easily caused the potential danger such as collagen calcification in connection agent.Research shows, even if glutaraldehyde is residual
When staying mass concentration as little as 310mg/L the most toxic, its functional group not reacted or collagen drop under the effect of enzyme
These functional groups discharged during solution all may cause cell-cytotoxic reaction, works the mischief to cultivating bacterium.And 1-(3-dimethylamino
Propyl group)-3-ethyl-carbodiimide hydrochloride/N-hydroxy-succinamide cross-linking agent has spy nontoxic, that biocompatibility is good
Point, applies and can show the most excellent performance in the modification of collagen protein.
The application for a patent for invention of Publication No. 102617213A discloses a kind of utilization compression straw and prepares cultivating champignon base
The method of matter, this culture matrix uses weight ratio to be the thick wood flour of 25-35%, the thin wood flour of 15-25%, the pressure being impregnated with of 25-35%
Contracting stalk particle, the wheat bran of 10-15%, the Gypsum Fibrosum of 1.0-1.5% are mixed with and form, and this invention culture matrix content of lignin is relatively
The utilization of height, beneficially mushroom mycelium, uses agricultural production waste material as cultivation matrix raw material simultaneously, improves environment and saving
Cost, but the cultivation matrix of this disclosure of the invention uses raw material the most single, easily causes nutrient unbalanced, needs extra nutritional thing
The interpolation of matter, simultaneously the highest with the biocompatibility of Lentinus Edodes, the extension with mycelia is improved without promoting for mushroom growth microenvironment
Enter effect, without the raising of notable surcharge, limit the scale application of invention substrate.
Summary of the invention
The object of the invention is contemplated to make up the defect of prior art, it is provided that a kind of Lentinus Edodes collagen promoting mycelial growth is planted
Training substrate.
The present invention is achieved by the following technical solutions:
A kind of Lentinus Edodes collagen cultivation matrix promoting mycelial growth, is prepared by the raw materials in: edible rice vinegar liquid 15-
18, glucose powder 2-3, calcium carbonate 1-2, earthworm excrement 42-46, soy sauce residue 13-15, calcium acetate 2-4, zinc sulfate 1-2, ammonium dihydrogen phosphate
3-4, pyrolusite powder 3-5, Corii Sus domestica waste material 45-47, sodium chloride powder 2-3, pepsin preparation 2-3,1-(3-dimethylamino third
Base)-3-ethyl-carbodiimide hydrochloride 1.5-1.8, N-hydroxy-succinamide 0.3-0.4,1% sodium chloride solution be appropriate,
PH2.5 acetum is appropriate, sodium hydroxide solution is appropriate, water is appropriate.
Specifically comprising the following steps that of the described Lentinus Edodes collagen cultivation matrix preparation method promoting mycelial growth
(1) add water after edible rice vinegar liquid, earthworm excrement, soy sauce residue being sufficiently mixed loose to material humidity, in retting fermentation 2-3 week, will send out
Ferment material squeezes and filters, and obtains fermentation liquid and fermentation siccative, is more individually taken out by fermentation liquid, collect the most afterwards bacterium mud precipitation and
High fermentation clear liquid, makes lyophilized powder by bacterium mud lyophilization, i.e. obtains fermentation siccative, fermentation clear liquid and lyophilized powder standby；
(2) in step 1 gained fermentation clear liquid, add calcium acetate, zinc sulfate, glucose powder, be heated to 74-78 DEG C, constantly stir
Mixing chelating 15-20 minute, be cooled to room temperature afterwards, add ammonium dihydrogen phosphate, calcium carbonate, stirring mixing, to uniformly, obtains nutrition
Liquid is standby；
(3) Corii Sus domestica waste material unhairing afterwash is shredded, join in 1% sodium chloride solution by the solid-to-liquid ratio of 1:5-6g/mL, soak
Clean with clear water after 5-6 hour, then it is molten that by the solid-to-liquid ratio of 1:15-18g/mL, gained skin bit is joined the acetic acid that pH value is 2.5
In liquid, and soak 8-10 hour under condition of ice bath, after completing, skin bit is smashed, now add the above-mentioned acetic acid of same volume
Solution, and add pepsin preparation, enzymolysis 12-15 hour, the most per hour stirring 5-7 minute, enzymolysis uses bilayer after terminating
Filtered through gauze, collects filtrate, is added thereto to sodium hydroxide solution regulation PH to 7-8, adds sodium chloride powder, stand 10-
12 hours, afterwards that solution is centrifugal 15-18 minute with 5000-5500 rev/min, collect precipitation, after drying, obtain collagen protein powder
Standby；
(4) by step 1 gained fermentation siccative, lyophilized powder, step 2 gained nutritional solution, step 3 gained collagen protein powder and soft
After manganese ore powder all mixes and is sufficiently stirred for, it is placed under 2-4 DEG C of environment, now adds 1-(3-dimethylamino-propyl)-3-second
Base carbodiimide hydrochloride and N-hydroxy-succinamide, cross-link 10-12 hour, be warmed to room temperature after completing, and obtains the present invention fragrant
Mushroom cultivation matrix.
The invention have the advantage that
Substrate of the present invention by extracting the glue by having reduced immunogenicity, biodegradability to collagen protein in Corii Sus domestica waste material
Former albumen is applied in the preparation of cultivation matrix, use simultaneously 1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride/
N-hydroxy-succinamide carries out crosslinking Treatment as cross-linking agent to collagen, makes denaturation temperature and the resistance to enzymolysis ability of collagen protein
Improving, water absorption rate and swelling ratio reduce, and improve its heat stability and structural stability, can not only provide certain propping up for Lentinus Edodes
Support and growth interface, the porous network structure that the interlaced arrangement of collagenous fiber bundle within collagen protein is formed simultaneously, also have
It is beneficial to beneficial microorganism carry and nutritional labeling fixing, the most beneficially the adhesion of mushroom mycelium and moving into, for Lentinus Edodes
Growth provide environment suitable, that nutrient is sufficient.
The mushroom cultivation substrate nutritional labeling of the present invention is comprehensive, and the use of edible rice vinegar liquid can extend Lentinus Edodes fruiting peak
Phase, coordinate the carbon source in the raw materials such as glucose powder, calcium carbonate, calcium ion easily to be absorbed by mycelia, mushroom fruiting body can be promoted
Plumpness, and the growth of beneficially mycelia, thus cultivate mycelium and the sporophore of stalwartness, enhance Lentinus Edodes resistance against diseases, improve
The product quality of Lentinus Edodes.
Detailed description of the invention
A kind of Lentinus Edodes collagen cultivation matrix promoting mycelial growth, is made up of the raw material of following weight portion (kg): edible rice
Vinegar liquid 15, glucose powder 2, calcium carbonate 1, earthworm excrement 42, soy sauce residue 13, calcium acetate 2, zinc sulfate 1, ammonium dihydrogen phosphate 3, pyrolusite powder
End 3, Corii Sus domestica waste material 45, sodium chloride powder 2, pepsin preparation 2,1-(3-dimethylamino-propyl)-3-ethyl carbodiimide salt
Hydrochlorate 1.5, N-hydroxy-succinamide 0.3,1% sodium chloride solution are appropriate, pH2.5 acetum is appropriate, sodium hydroxide solution is fitted
Amount, water are appropriate.
Specifically comprising the following steps that of the described Lentinus Edodes collagen cultivation matrix preparation method promoting mycelial growth
(1) adding water after edible rice vinegar liquid, earthworm excrement, soy sauce residue being sufficiently mixed loose to material humidity, retting is fermented 2 weeks, will fermentation
Material squeezing is also filtered, and obtains fermentation liquid and fermentation siccative, is more individually taken out by fermentation liquid, collect the most afterwards bacterium mud precipitation and on
Layer fermentation clear liquid, makes lyophilized powder by bacterium mud lyophilization, i.e. obtains fermentation siccative, fermentation clear liquid and lyophilized powder standby；
(2) in step 1 gained fermentation clear liquid, add calcium acetate, zinc sulfate, glucose powder, be heated to 74 DEG C, be stirred continuously
Chelating 15 minutes, be cooled to room temperature afterwards, add ammonium dihydrogen phosphate, calcium carbonate, stirring mixing, to uniformly, obtains nutritional solution standby
With；
(3) Corii Sus domestica waste material unhairing afterwash is shredded, join in 1% sodium chloride solution by the solid-to-liquid ratio of 1:5g/mL, soak 5 little
Shi Houyong clear water is cleaned, then is joined in the acetum that pH value is 2.5 by the solid-to-liquid ratio of 1:15g/mL by gained skin bit, and
Soak 8 hours under condition of ice bath, after completing, skin bit is smashed, now add the above-mentioned acetum of same volume, and add
Pepsin preparation, enzymolysis 12 hours, the most per hour stirring 5 minutes, enzymolysis filters with double gauze after terminating, collects filter
Liquid, be added thereto to sodium hydroxide solution regulation PH to 7, add sodium chloride powder, stand 10 hours, afterwards by solution with
5000 revs/min are centrifuged 15 minutes, collect precipitation, obtain collagen protein powder standby after drying；
(4) by step 1 gained fermentation siccative, lyophilized powder, step 2 gained nutritional solution, step 3 gained collagen protein powder and soft
After manganese ore powder all mixes and is sufficiently stirred for, it is placed under 2 DEG C of environment, now adds 1-(3-dimethylamino-propyl)-3-ethyl
Carbodiimide hydrochloride and N-hydroxy-succinamide, cross-link 10 hours, be warmed to room temperature, obtain cultivating champignon of the present invention after completing
Substrate.
In order to further illustrate the using value of the present invention, inventor uses substrate of the present invention and commercially available generic media respectively
Carrying out the cultivating and growing of Lentinus Edodes, in addition to using substrate difference, other management methods are the most identical, and data measured is substrate of the present invention institute
The more conventional substrate of biological transformation ratio of Lentinus Edodes improves 5.2%, and the output increased of finished product mushroom 16.8%, massee fruiting bodies simultaneously
Stem is pure white, and mushroom shape is preferable, illustrates that Lentinus Edodes substrate of the present invention has preferable using effect.
Claims (2)
1. the Lentinus Edodes collagen cultivation matrix promoting mycelial growth, it is characterised in that be prepared by the raw materials in: food
With rice vinegar liquid 15-18, glucose powder 2-3, calcium carbonate 1-2, earthworm excrement 42-46, soy sauce residue 13-15, calcium acetate 2-4, zinc sulfate 1-
2, ammonium dihydrogen phosphate 3-4, pyrolusite powder 3-5, Corii Sus domestica waste material 45-47, sodium chloride powder 2-3, pepsin preparation 2-3,1-
(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride 1.5-1.8, N-hydroxy-succinamide 0.3-0.4,1% sodium chloride
Solution is appropriate, pH2.5 acetum is appropriate, sodium hydroxide solution is appropriate, water is appropriate.
2. according to the Lentinus Edodes collagen cultivation matrix promoting mycelial growth described in claims 1, it is characterised in that preparation method
Specifically comprise the following steps that
(1) add water after edible rice vinegar liquid, earthworm excrement, soy sauce residue being sufficiently mixed loose to material humidity, in retting fermentation 2-3 week, will send out
Ferment material squeezes and filters, and obtains fermentation liquid and fermentation siccative, is more individually taken out by fermentation liquid, collect the most afterwards bacterium mud precipitation and
High fermentation clear liquid, makes lyophilized powder by bacterium mud lyophilization, i.e. obtains fermentation siccative, fermentation clear liquid and lyophilized powder standby；
(2) in step 1 gained fermentation clear liquid, add calcium acetate, zinc sulfate, glucose powder, be heated to 74-78 DEG C, constantly
Stirring chelating 15-20 minute, is cooled to room temperature afterwards, adds ammonium dihydrogen phosphate, calcium carbonate, and stirring mixing, to uniformly, obtains battalion
Nutrient solution is standby；
(3) Corii Sus domestica waste material unhairing afterwash is shredded, join in 1% sodium chloride solution by the solid-to-liquid ratio of 1:5-6g/mL, soak
Clean with clear water after 5-6 hour, then it is molten that by the solid-to-liquid ratio of 1:15-18g/mL, gained skin bit is joined the acetic acid that pH value is 2.5
In liquid, and soak 8-10 hour under condition of ice bath, after completing, skin bit is smashed, now add the above-mentioned acetic acid of same volume
Solution, and add pepsin preparation, enzymolysis 12-15 hour, the most per hour stirring 5-7 minute, enzymolysis uses bilayer after terminating
Filtered through gauze, collects filtrate, is added thereto to sodium hydroxide solution regulation PH to 7-8, adds sodium chloride powder, stand 10-
12 hours, afterwards that solution is centrifugal 15-18 minute with 5000-5500 rev/min, collect precipitation, after drying, obtain collagen protein powder
Standby；
(4) by step 1 gained fermentation siccative, lyophilized powder, step 2 gained nutritional solution, step 3 gained collagen protein powder and
After pyrolusite powder all mixes and is sufficiently stirred for, it is placed under 2-4 DEG C of environment, now adds 1-(3-dimethylamino-propyl)-3-
Ethyl-carbodiimide hydrochloride and N-hydroxy-succinamide, cross-link 10-12 hour, be warmed to room temperature, obtain the present invention after completing
Mushroom cultivation substrate.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610645691.XA CN106316576A (en) | 2016-08-09 | 2016-08-09 | Mushroom collagen culture substrate for facilitating hypha growth |
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| CN201610645691.XA CN106316576A (en) | 2016-08-09 | 2016-08-09 | Mushroom collagen culture substrate for facilitating hypha growth |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107047213A (en) * | 2017-05-27 | 2017-08-18 | 界首市大自然养殖专业合作社 | A kind of implantation methods for improving North-Anhui district large-scale planting lycium ruthenicum nutritive value |
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| CN101434642A (en) * | 2007-11-15 | 2009-05-20 | 黑龙江大学 | Method for preparing non-denaturation hogskin insoluble collagen powder |
| CN103961749A (en) * | 2014-05-07 | 2014-08-06 | 无锡贝迪生物工程有限公司 | Method for preparing collagen protein/silica membrane double-layer stent |
| CN104945118A (en) * | 2015-06-16 | 2015-09-30 | 王金生 | Method for preparing mushroom culture medium |
| CN105218239A (en) * | 2015-10-16 | 2016-01-06 | 淮北市振兴食药用菌研究所 | A kind of mushroom cultivation substrate |
| CN105461437A (en) * | 2015-12-17 | 2016-04-06 | 凤台县星展食用菌有限公司 | Hericium erinaceus culture medium and preparation method thereof |
| CN105693382A (en) * | 2016-01-25 | 2016-06-22 | 苗娥 | Special organic fertilizer for calliandra haematocephala and preparation method thereof |
-
2016
- 2016-08-09 CN CN201610645691.XA patent/CN106316576A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101434642A (en) * | 2007-11-15 | 2009-05-20 | 黑龙江大学 | Method for preparing non-denaturation hogskin insoluble collagen powder |
| CN103961749A (en) * | 2014-05-07 | 2014-08-06 | 无锡贝迪生物工程有限公司 | Method for preparing collagen protein/silica membrane double-layer stent |
| CN104945118A (en) * | 2015-06-16 | 2015-09-30 | 王金生 | Method for preparing mushroom culture medium |
| CN105218239A (en) * | 2015-10-16 | 2016-01-06 | 淮北市振兴食药用菌研究所 | A kind of mushroom cultivation substrate |
| CN105461437A (en) * | 2015-12-17 | 2016-04-06 | 凤台县星展食用菌有限公司 | Hericium erinaceus culture medium and preparation method thereof |
| CN105693382A (en) * | 2016-01-25 | 2016-06-22 | 苗娥 | Special organic fertilizer for calliandra haematocephala and preparation method thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107047213A (en) * | 2017-05-27 | 2017-08-18 | 界首市大自然养殖专业合作社 | A kind of implantation methods for improving North-Anhui district large-scale planting lycium ruthenicum nutritive value |
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