CN106258482A - A kind of Lentinus Edodes collagen cultivation matrix utilizing cinder to prepare - Google Patents
A kind of Lentinus Edodes collagen cultivation matrix utilizing cinder to prepare Download PDFInfo
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- CN106258482A CN106258482A CN201610645877.5A CN201610645877A CN106258482A CN 106258482 A CN106258482 A CN 106258482A CN 201610645877 A CN201610645877 A CN 201610645877A CN 106258482 A CN106258482 A CN 106258482A
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- powder
- fermentation
- cinder
- lentinus edodes
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Medicines Containing Plant Substances (AREA)
- Mushroom Cultivation (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a kind of Lentinus Edodes collagen cultivation matrix utilizing cinder to prepare, be prepared by the raw materials in: cinder 46 48, furfural dregs 22 26, Os Sus domestica powder 9 11, wood vinegar 46, magnesium sulfate 12, Borax 34, ground phosphate rock 23, Corii Sus domestica waste material 43 46, sodium chloride powder 23, pepsin preparation 23,1 (3 dimethylamino-propyl) 3 ethyl-carbodiimide hydrochlorides, N N-Hydroxysuccinimide 1.8 2.0,1% sodium chloride solution are appropriate 0.4 0.6, sodium hydroxide solution is appropriate, water is appropriate；The mushroom cultivation substrate of the present invention uses cinder as primary raw material, not only there is substantial amounts of nitrogen, carbon source, the cultivation matrix simultaneously built has the physical and chemical indexs such as preferable retentiveness, breathability, the Lentinus Edodes not only yield of cultivation is high, and regularity is preferable, it is easy to batch production operation, can be in the large scale application of cultivating champignon field.
Description
Technical field
The present invention relates to fungus growing technique field, particularly relate to a kind of Lentinus Edodes collagen cultivation base utilizing cinder to prepare
Matter.
Background technology
Lentinus Edodes is commonly called as China mushroom, is that mycophagy medical material is cultivated in a kind of famous using, has long cultivation in China
Historical tradition, the nutritive value abundant because of it and medical value and deeply liked by consumer, become the second adult in the world
The edible fungi of work cultivation, due to the fast development of industry, the raw material of Lentinus Edodes substituting stuff cultivation traditional matrices is the most exhausted, needs actively
Explore the approach substituting substrate.On the other hand, along with the raising of people's living standard, it is desirable to Lentinus Edodes nutritive value and medicinal
It is worth and also can be improved, so needing to provide the mushroom cultivation substrate of a kind of high-quality.
Collagen protein as a kind of biomacromolecule, have natural hypotoxicity, hypoimmunity, bootable mycelial growth with
And the preferable advantage such as biocompatibility, its extracting method is simple simultaneously, and raw material sources extensively and cheap, are discarded by slaughterhouse
Corii Sus domestica can carry out scale extraction, it has a very high potential as the New-type matrix material of cultivating champignon, but Non-crosslinked
The collagen protein of moditied processing often has that degradation rate is too fast, deformation temperature is low, easily shrink deformation and mechanical performance
The shortcomings such as deficiency, need by the cross-linking modified mechanical performance improving collagen protein and anti-degradation capability.
At present, the method that collagen-stabilizedization processes mainly is cross-linked by method physically or chemically.Wherein,
Physical method has vacuum dehydrothermal, ultraviolet and C ray crosslinking etc., and the advantage of these methods is not introduce toxic chemical substance,
Can keep the biocompatibility that collagen is good, but individually use physical crosslinking tend not to obtain homogeneous, preferably crosslinking strong
Degree, therefore Chemical Crosslinking Methods is more widely applied.
Common chemical cross-linking agent has aldehydes, Carbodiimides, bis-epoxy class material and the different hydrochlorate of cyanogen etc..Wherein,
Glutaraldehyde is the cross-linking agent that a class is widely used in collagen base biological material, can reduce the antigenicity of collagen, but glutaraldehyde is handed over
There is the reduction collagenous biological compatibility and be easily caused the potential danger such as collagen calcification in connection agent.Research shows, even if glutaraldehyde is residual
When staying mass concentration as little as 310mg/L the most toxic, its functional group not reacted or collagen drop under the effect of enzyme
These functional groups discharged during solution all may cause cell-cytotoxic reaction, works the mischief to cultivating bacterium.And 1-(3-dimethylamino
Propyl group)-3-ethyl-carbodiimide hydrochloride/N-hydroxy-succinamide cross-linking agent has spy nontoxic, that biocompatibility is good
Point, applies and can show the most excellent performance in the modification of collagen protein.
The application for a patent for invention of Publication No. 102617213A discloses a kind of utilization compression straw and prepares cultivating champignon base
The method of matter, this culture matrix uses weight ratio to be the thick wood flour of 25-35%, the thin wood flour of 15-25%, the pressure being impregnated with of 25-35%
Contracting stalk particle, the wheat bran of 10-15%, the Gypsum Fibrosum of 1.0-1.5% are mixed with and form, and this invention culture matrix content of lignin is relatively
The utilization of height, beneficially mushroom mycelium, uses agricultural production waste material as cultivation matrix raw material simultaneously, improves environment and saving
Cost, but the cultivation matrix of this disclosure of the invention uses raw material the most single, easily causes nutrient unbalanced, needs extra nutritional thing
The interpolation of matter, simultaneously the highest with the biocompatibility of Lentinus Edodes, the extension with mycelia is improved without promoting for mushroom growth microenvironment
Enter effect, without the raising of notable surcharge, limit the scale application of invention substrate.
Summary of the invention
The object of the invention is contemplated to make up the defect of prior art, it is provided that a kind of Lentinus Edodes collagen utilizing cinder to prepare is planted
Training substrate.
The present invention is achieved by the following technical solutions:
A kind of Lentinus Edodes collagen cultivation matrix utilizing cinder to prepare, is prepared by the raw materials in: cinder 46-48, furfural
Slag 22-26, Os Sus domestica powder 9-11, wood vinegar 4-6, magnesium sulfate 1-2, Borax 3-4, ground phosphate rock 2-3, Corii Sus domestica waste material 43-46, sodium chloride
Powder 2-3, pepsin preparation 2-3,1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride, N-hydroxysuccinimidyl acyl
Imines 1.8-2.0,1% sodium chloride solution appropriate 0.4-0.6, pH2.5 acetum is appropriate, sodium hydroxide solution is appropriate, water is suitable
Amount.
Specifically comprising the following steps that of the described Lentinus Edodes collagen cultivation matrix preparation method utilizing cinder to prepare
(1) being sufficiently mixed with furfural dregs, Os Sus domestica powder after cinder being ground into particulate, add water loose to material humidity, retting is fermented
20-22 days, fermentation material squeezed and filters, obtaining fermentation liquid and fermentation siccative, then fermentation liquid is individually taken out, receive the most afterwards
Collection bacterium mud precipitation and high fermentation clear liquid, make lyophilized powder by bacterium mud lyophilization, i.e. obtain fermentation siccative, fermentation clear liquid and freeze
Dry powder is standby；
(2) in step 1 gained fermentation clear liquid, add wood vinegar, magnesium sulfate, be heated to 70-75 DEG C, be stirred continuously chelating 20-30
Minute, it being cooled to room temperature afterwards, add Borax, stirring mixing, to uniformly, obtains nutritional solution standby；
(3) Corii Sus domestica waste material unhairing afterwash is shredded, join in 1% sodium chloride solution by the solid-to-liquid ratio of 1:5-6g/mL, soak
Clean with clear water after 5-6 hour, then it is molten that by the solid-to-liquid ratio of 1:15-18g/mL, gained skin bit is joined the acetic acid that pH value is 2.5
In liquid, and soak 8-10 hour under condition of ice bath, after completing, skin bit is smashed, now add the above-mentioned acetic acid of same volume
Solution, and add pepsin preparation, enzymolysis 12-15 hour, the most per hour stirring 5-7 minute, enzymolysis uses bilayer after terminating
Filtered through gauze, collects filtrate, is added thereto to sodium hydroxide solution regulation PH to 7-8, adds sodium chloride powder, stand 10-
12 hours, afterwards that solution is centrifugal 15-18 minute with 5000-5500 rev/min, collect precipitation, after drying, obtain collagen protein powder
Standby；
(4) by step 1 gained fermentation siccative, lyophilized powder, step 2 gained nutritional solution, step 3 gained collagen protein powder and phosphorus
After breeze all mixes and is sufficiently stirred for, it is placed under 2-4 DEG C of environment, now adds 1-(3-dimethylamino-propyl)-3-ethyl carbon
Diimmonium salt hydrochlorate and N-hydroxy-succinamide, cross-link 10-12 hour, be warmed to room temperature after completing, and obtains Lentinus Edodes of the present invention and plants
Training substrate.
The invention have the advantage that
Substrate of the present invention by extracting the glue by having reduced immunogenicity, biodegradability to collagen protein in Corii Sus domestica waste material
Former albumen is applied in the preparation of cultivation matrix, use simultaneously 1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride/
N-hydroxy-succinamide carries out crosslinking Treatment as cross-linking agent to collagen, makes denaturation temperature and the resistance to enzymolysis ability of collagen protein
Improving, water absorption rate and swelling ratio reduce, and improve its heat stability and structural stability, can not only provide certain propping up for Lentinus Edodes
Support and growth interface, the porous network structure that the interlaced arrangement of collagenous fiber bundle within collagen protein is formed simultaneously, also have
It is beneficial to beneficial microorganism carry and nutritional labeling fixing, the most beneficially the adhesion of mushroom mycelium and moving into, for Lentinus Edodes
Growth provide environment suitable, that nutrient is sufficient.
The mushroom cultivation substrate of the present invention uses cinder as primary raw material, not only has substantial amounts of nitrogen, carbon source, simultaneously structure
The cultivation matrix built has the physical and chemical indexs such as preferable retentiveness, breathability, and the Lentinus Edodes of cultivation not only yield is high, and regularity
Preferably, it is simple to batch production operates, can be in the large scale application of cultivating champignon field.
Detailed description of the invention
A kind of Lentinus Edodes collagen cultivation matrix utilizing cinder to prepare, is made up of the raw material of following weight portion (kg): cinder 46,
Furfural dregs 22, Os Sus domestica powder 9, wood vinegar 4, magnesium sulfate 1, Borax 3, ground phosphate rock 2, Corii Sus domestica waste material 43, sodium chloride powder 2, pepsin
Enzyme preparation 2,1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride, N-hydroxy-succinamide 1.8,1% sodium chloride
Solution is appropriate 0.4, pH2.5 acetum is appropriate, sodium hydroxide solution is appropriate, water is appropriate.
Specifically comprising the following steps that of the described Lentinus Edodes collagen cultivation matrix preparation method utilizing cinder to prepare
(1) being sufficiently mixed with furfural dregs, Os Sus domestica powder after cinder being ground into particulate, add water loose to material humidity, retting is fermented
20 days, fermentation material squeezed and filters, obtaining fermentation liquid and fermentation siccative, then fermentation liquid is individually taken out, collect the most afterwards
Bacterium mud precipitation and high fermentation clear liquid, make lyophilized powder by bacterium mud lyophilization, i.e. obtains ferment siccative, fermentation clear liquid and lyophilizing
Powder is standby；
(2) in step 1 gained fermentation clear liquid, add wood vinegar, magnesium sulfate, be heated to 70 DEG C, be stirred continuously chelating 20 minutes,
Being cooled to room temperature afterwards, add Borax, stirring mixing, to uniformly, obtains nutritional solution standby；
(3) Corii Sus domestica waste material unhairing afterwash is shredded, join in 1% sodium chloride solution by the solid-to-liquid ratio of 1:5g/mL, soak 5 little
Shi Houyong clear water is cleaned, then is joined in the acetum that pH value is 2.5 by the solid-to-liquid ratio of 1:15g/mL by gained skin bit, and
Soak 8 hours under condition of ice bath, after completing, skin bit is smashed, now add the above-mentioned acetum of same volume, and add
Pepsin preparation, enzymolysis 12 hours, the most per hour stirring 5 minutes, enzymolysis filters with double gauze after terminating, collects filter
Liquid, be added thereto to sodium hydroxide solution regulation PH to 7, add sodium chloride powder, stand 10 hours, afterwards by solution with
5000 revs/min are centrifuged 15 minutes, collect precipitation, obtain collagen protein powder standby after drying；
(4) by step 1 gained fermentation siccative, lyophilized powder, step 2 gained nutritional solution, step 3 gained collagen protein powder and phosphorus
After breeze all mixes and is sufficiently stirred for, it is placed under 2 DEG C of environment, now adds 1-(3-dimethylamino-propyl)-3-ethyl carbon two
Inferior amine salt hydrochlorate and N-hydroxy-succinamide, cross-link 10 hours, be warmed to room temperature after completing, obtain cultivating champignon base of the present invention
Matter.
In order to further illustrate the using value of the present invention, inventor uses substrate of the present invention and commercially available generic media respectively
Carrying out the cultivating and growing of Lentinus Edodes, in addition to using substrate difference, other management methods are the most identical, and data measured is substrate of the present invention institute
The more conventional substrate of biological transformation ratio of Lentinus Edodes improves 5.7%, and the output increased of finished product mushroom 13.3%, massee fruiting bodies simultaneously
Stem is pure white, and mushroom shape is preferable, illustrates that Lentinus Edodes substrate of the present invention has preferable using effect.
Claims (2)
1. the Lentinus Edodes collagen cultivation matrix that a kind utilizes cinder to prepare, it is characterised in that be prepared by the raw materials in: coal
Slag 46-48, furfural dregs 22-26, Os Sus domestica powder 9-11, wood vinegar 4-6, magnesium sulfate 1-2, Borax 3-4, ground phosphate rock 2-3, Corii Sus domestica waste material
43-46, sodium chloride powder 2-3, pepsin preparation 2-3,1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride,
N-hydroxy-succinamide 1.8-2.0,1% sodium chloride solution appropriate 0.4-0.6, pH2.5 acetum is appropriate, sodium hydroxide is molten
Liquid is appropriate, water is appropriate.
2. according to utilizing Lentinus Edodes collagen cultivation matrix prepared by cinder described in claims 1, it is characterised in that preparation method
Specifically comprise the following steps that
(1) being sufficiently mixed with furfural dregs, Os Sus domestica powder after cinder being ground into particulate, add water loose to material humidity, retting is fermented
20-22 days, fermentation material squeezed and filters, obtaining fermentation liquid and fermentation siccative, then fermentation liquid is individually taken out, receive the most afterwards
Collection bacterium mud precipitation and high fermentation clear liquid, make lyophilized powder by bacterium mud lyophilization, i.e. obtain fermentation siccative, fermentation clear liquid and freeze
Dry powder is standby；
(2) in step 1 gained fermentation clear liquid, add wood vinegar, magnesium sulfate, be heated to 70-75 DEG C, be stirred continuously chelating 20-
30 minutes, being cooled to room temperature afterwards, add Borax, stirring mixing, to uniformly, obtains nutritional solution standby；
(3) Corii Sus domestica waste material unhairing afterwash is shredded, join in 1% sodium chloride solution by the solid-to-liquid ratio of 1:5-6g/mL, soak
Clean with clear water after 5-6 hour, then it is molten that by the solid-to-liquid ratio of 1:15-18g/mL, gained skin bit is joined the acetic acid that pH value is 2.5
In liquid, and soak 8-10 hour under condition of ice bath, after completing, skin bit is smashed, now add the above-mentioned acetic acid of same volume
Solution, and add pepsin preparation, enzymolysis 12-15 hour, the most per hour stirring 5-7 minute, enzymolysis uses bilayer after terminating
Filtered through gauze, collects filtrate, is added thereto to sodium hydroxide solution regulation PH to 7-8, adds sodium chloride powder, stand 10-
12 hours, afterwards that solution is centrifugal 15-18 minute with 5000-5500 rev/min, collect precipitation, after drying, obtain collagen protein powder
Standby；
(4) by step 1 gained fermentation siccative, lyophilized powder, step 2 gained nutritional solution, step 3 gained collagen protein powder and
After ground phosphate rock all mixes and is sufficiently stirred for, it is placed under 2-4 DEG C of environment, now adds 1-(3-dimethylamino-propyl)-3-ethyl
Carbodiimide hydrochloride and N-hydroxy-succinamide, cross-link 10-12 hour, be warmed to room temperature after completing, obtain Lentinus Edodes of the present invention
Cultivation matrix.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610645877.5A CN106258482A (en) | 2016-08-09 | 2016-08-09 | A kind of Lentinus Edodes collagen cultivation matrix utilizing cinder to prepare |
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| CN201610645877.5A CN106258482A (en) | 2016-08-09 | 2016-08-09 | A kind of Lentinus Edodes collagen cultivation matrix utilizing cinder to prepare |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115281054A (en) * | 2022-08-05 | 2022-11-04 | 中国农业科学院都市农业研究所 | Solid matrix manufacturing method and application thereof |
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| CN1915437A (en) * | 2005-08-15 | 2007-02-21 | 上海其胜生物制剂有限公司 | Technique for preparing sponge produced from collagen |
| CN103627761A (en) * | 2013-10-22 | 2014-03-12 | 浙江省海洋开发研究院 | Method for preparing collagen peptide rich in hydroxyproline |
| CN103961749A (en) * | 2014-05-07 | 2014-08-06 | 无锡贝迪生物工程有限公司 | Method for preparing collagen protein/silica membrane double-layer stent |
| CN104016773A (en) * | 2014-05-07 | 2014-09-03 | 合肥福泉现代农业科技有限公司 | Shiitake culture medium prepared from tea seed husk and preparation method thereof |
| CN104447092A (en) * | 2014-12-03 | 2015-03-25 | 大新县生产力促进中心 | Special composite fertilizer for broadleaf holly leaves and production method of composite fertilizer |
-
2016
- 2016-08-09 CN CN201610645877.5A patent/CN106258482A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1915437A (en) * | 2005-08-15 | 2007-02-21 | 上海其胜生物制剂有限公司 | Technique for preparing sponge produced from collagen |
| CN103627761A (en) * | 2013-10-22 | 2014-03-12 | 浙江省海洋开发研究院 | Method for preparing collagen peptide rich in hydroxyproline |
| CN103961749A (en) * | 2014-05-07 | 2014-08-06 | 无锡贝迪生物工程有限公司 | Method for preparing collagen protein/silica membrane double-layer stent |
| CN104016773A (en) * | 2014-05-07 | 2014-09-03 | 合肥福泉现代农业科技有限公司 | Shiitake culture medium prepared from tea seed husk and preparation method thereof |
| CN104447092A (en) * | 2014-12-03 | 2015-03-25 | 大新县生产力促进中心 | Special composite fertilizer for broadleaf holly leaves and production method of composite fertilizer |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115281054A (en) * | 2022-08-05 | 2022-11-04 | 中国农业科学院都市农业研究所 | Solid matrix manufacturing method and application thereof |
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Application publication date: 20170104 |