CN106259830A - A kind of aquatic products composite fresh-keeping agent prescription - Google Patents
A kind of aquatic products composite fresh-keeping agent prescription Download PDFInfo
- Publication number
- CN106259830A CN106259830A CN201610663551.5A CN201610663551A CN106259830A CN 106259830 A CN106259830 A CN 106259830A CN 201610663551 A CN201610663551 A CN 201610663551A CN 106259830 A CN106259830 A CN 106259830A
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- Prior art keywords
- aquatic products
- spice
- extract
- products composite
- composite preservative
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B4/00—General methods for preserving meat, sausages, fish or fish products
- A23B4/14—Preserving with chemicals not covered by groups A23B4/02 or A23B4/12
- A23B4/18—Preserving with chemicals not covered by groups A23B4/02 or A23B4/12 in the form of liquids or solids
- A23B4/20—Organic compounds; Microorganisms; Enzymes
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B4/00—General methods for preserving meat, sausages, fish or fish products
- A23B4/14—Preserving with chemicals not covered by groups A23B4/02 or A23B4/12
- A23B4/18—Preserving with chemicals not covered by groups A23B4/02 or A23B4/12 in the form of liquids or solids
- A23B4/20—Organic compounds; Microorganisms; Enzymes
- A23B4/22—Microorganisms; Enzymes; Antibiotics
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
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Abstract
The present invention relates to a kind of aquatic products composite fresh-keeping agent prescription, it is characterized in that, containing following components in described every liter of distilled water of aquatic products composite preservative: lysozyme 0.4 0.6g, tea polyphenols 2.5 3.5g, carboxymethyl chitosan 16 20g, nisin 10 14g, potassium sorbate 25 35g, sodium alginate 17 23g, lactic acid bacteria 20 30g, propolis extract 1.5 2.5g, spice extract 10 14g；The raw material of described spice extracting solution is Rhizoma Alpiniae Officinarum, Bulbus Allii, Bulbus Allii Cepae, Cortex cinnamomi japonici (Ramulus Cinnamomi), Flos Caryophylli and Herba Rosmarini Officinalis.Aquatic products composite preservative of the present invention uses bio-preservative compounding to form, safety non-toxic, and preparation, application process are simple, and suppression microorganism effect highlights, and good refreshing effect can be greatly prolonged the shelf life of cold-storage water product, have important industrial value.
Description
Technical field
The present invention relates to a kind of aquatic products composite fresh-keeping agent prescription, belong to Aquatic product technical field of preservation of fresh, particularly to one
Use bio-preservative compounding to form, safety non-toxic, preparation, application process are simple, and suppression microorganism effect highlights, fresh-keeping effect
Good, the shelf life of cold-storage water product can be greatly prolonged, there is the aquatic products composite fresh-keeping agent prescription of important industrial value.
Background technology
Along with the growth of world population, food supply not only requested number is protected, to the quality of food, matter structure,
The requirement of nutrition, local flavor and shelf life is more and more higher.Than fowl poultry kind food, aquatic product food is as high protein, low fat
" plain boiled pork " of fat, obtains cognition in the world.China is the country that output of aquatic products is maximum in the world, consumption figure is most,
Have extremely abundant aquatic products resource, increase year by year recently as Export of Chinese Aquatic Products amount, the development of aquatic products industry
Space is more wide.
The provincialism of fish production and seasonality are very strong, and fishery is wide in variety and yield is high, are limited by storage, traffic condition
System, aquatic products are easily infected by putrefaction bacteria in storage and lose fresh rotten, and gradually losing nutritive value and commodity value causes
The wasting of resources greatly and huge economic loss, thus aquatic products is fresh-keeping particularly significant.Aquatic products storage technique
Exploitation, is cultivation, fishing activity continuity, the necessary guarantee of processing enterprise's extra earning, is that whole fishery field is indispensable
Ingredient.
Aquatic products generally use and either physically or chemically delay or suppress it corrupt, to keep its fresh state, maintain relatively
High quality, the most conventional preservation method has preservation by low temperature, controlled atmosphere and chemical preservative fresh-keeping.But these are fresh-keeping
Technology respectively has certain limitation, utilizes low temperature control enzymatic activity and suppression microbial reproduction to carry out fresh-keeping, can be because freezing speed
Aquatic products muscular tissue is damaged by the formation of degree, variations in temperature and big ice crystal；Controlled atmosphere storage is because of complex operation, investment
The reason such as big is restricted in small business uses；Chemical preservative is fresh-keeping may be because of remaining in environment and human body
Bring a certain degree of potential hazard.
In recent years, the new biological way of keeping fresh as raw material with natural extract or secretions risen, day by day cause people
Concern.Biological way of keeping fresh, compared with tradition preservation method, has a series of advantage, such as little, the sky to human body toxic and side effects
The most safely, the quality impact on aquatic products is little, fungistatic effect is prominent, so receiving the generally high praise of people.But, at present
Do not have any bio-preservative can effectively suppress and kill all microorganisms, thus be used in all aquatic products safely.
Single creature antistaling agent self has good antibacterial antioxygenic property, but antibiotic property emphasizes particularly on different fields a little.For aquatic products corruption mistake
The microbial community structure feature of journey, theoretical according to fence, the bio-preservative of difference in functionality is combined, there is different fresh-keeping spy
The bio-preservative of point be combined with each other and realizes having complementary advantages and Synergistic, forms a kind of efficient composite preservative, can be more
Keep aquatic products quality, Shelf-life comprehensively.
Summary of the invention
For solving technical problem present in background technology, the present invention proposes a kind of to use bio-preservative compounding to form,
Safety non-toxic, simply, suppression microorganism effect highlights for preparation, application process, and good refreshing effect can be greatly prolonged cold preservation Aquatic product
The shelf life of product, has the aquatic products composite fresh-keeping agent prescription of important industrial value.
It is an object of the invention to solve by the following technical programs:
A kind of aquatic products composite preservative, it is characterised in that containing following in described every liter of distilled water of aquatic products composite preservative
Component: lysozyme 0.4-0.6g, tea polyphenols 2.5-3.5g, carboxymethyl chitosan 16-20g, nisin 10-14g, Pyrusussuriensis
Acid potassium 25-35g, sodium alginate 17-23g, lactic acid bacteria 20-30g, propolis extract 1.5-2.5g, spice extract 10-14g.
Described aquatic products composite preservative optimum formula is containing following components in every liter of distilled water: lysozyme 0.5g, tea
Polyphenol 3g, carboxymethyl chitosan 18g, nisin 12g, potassium sorbate 30g, sodium alginate 20g, lactic acid bacteria 25g, propolis
Extract 2g, spice extract 12g.
The extracting method of described propolis extract is: by propolis freezing and pulverizing remove impurity, with supercritical extraction instrument supercritical CO2
Extraction, extracting pressure controls to be 30-35MPa, and extraction temperature controls as 35-40 DEG C, CO2Flow-control is 16-22L/h, extraction
Time controls to be 3.5-4.5h, and knockout tower discharging obtains the propolis extract of yellow paste.
The raw material of described spice extract be weight ratio be the Rhizoma Alpiniae Officinarum of 5-7:4-6:3-5:2-3:4-6:4-5, big
Bulbus Allii, Bulbus Allii Cepae, Cortex cinnamomi japonici (Ramulus Cinnamomi), Flos Caryophylli, Herba Rosmarini Officinalis spice combinations of substances.
The extracting method of described spice extract is: is put into by spice in air dry oven and dries, and tissue is smash at a high speed
Broken machine adds 95% ethanol after smashing to pieces, solid-liquid ratio controls to shake 12h for 1g:10ml, 100r/min constant-temperature table, with filter paper filtering,
Residue adds 95% ethanol by solid-liquid ratio 1g:4ml, crosses and filter filtering residue after bringing up again 12h, is merged by twice extracting solution, is placed in rotation and steams
Send out decompression distillation in instrument, obtain spice extract.
The temperature that described spice is dried, constant-temperature table shakes, Rotary Evaporators decompression is distilled is all less than 50 DEG C.
The present invention has the following advantages compared to existing technology:
The aquatic products composite preservative of the present invention, preferably lysozyme, tea polyphenols, carboxymethyl chitosan, nisin, mountain
Potassium sorbate, sodium alginate, lactic acid bacteria, propolis extract.Wherein lysozyme can destroy in cell wall N-acetyl Fructus Vitis viniferae in Peptidoglycan
β between osamine and-acetylmuramic acid-Isosorbide-5-Nitrae glycosidic bond, and then under ICP effect, make cell rupture, especially to leather
Bacillus subtilis in Lan Shi positive bacteria, micrococcus radiodurans have stronger decomposition, to escherichia coli, P. vulgaris and pair
The gram negative bacterias such as hemolytic vibrio also have a certain degree of dissolution, and lysozyme is in selectively killing microorganism
Time, not acting on other materials in food, this guarantees the original nutritional labeling of food and do not suffer a loss, human body is with dynamic simultaneously
Thing cell is acellular wall construction is also without Peptidoglycan, therefore lysozyme is to the effect of human non-toxic's property；Tea polyphenols mainly contains catechin,
The important active substances such as anthocyanidin, phenolic acid and flavone compound, it is possible to effectively remove free radical, using the teaching of the invention it is possible to provide Hydrogen Proton, from
And the oxidation reaction suppressing fat free chain reaction to cause, reaching fresh-keeping effect, tea polyphenols also has scavenging capacity in addition
Oxygen, chelated metal ions and combine the effect such as oxidase；The cell membrane effect that carboxymethyl chitosan is electronegative with antibacterial, absorption
With coagulation antibacterial, penetration cell wall enters intracellular simultaneously, upsets the metabolism of antibacterial and synthesis and has antibacterial action, right
Staphylococcus aureus, escherichia coli, small intestinal knot point Yale Salmonella, Salmonella typhimurium and Liszt monokaryon hypertrophy bacterium, all have
Stronger inhibitory action, additionally, carboxymethyl chitosan also has blood fat reducing and prevents and treats arteriosclerosis, antiviral, antitumor, anticoagulant
Blood, blood sugar lowering, radioprotective isoreactivity, have prominent health-care effect；Nisin can be with bactoprenol-Jiao on cell membrane
Phosphoric acid--acetylmuramic acid-pentapeptide-N-Acetyl-D-glucosamine molecule combines, and forms hole complex, on the one hand, hole is combined
Thing hinders the normal synthesis of cell wall after being formed, cause cell death, and on the other hand, hole complex can be with cell membrane effect
Form perforation lanes, make intracellular matter run off, thus cause cell death, to gram positive bacteria, including staphylococcus, chain
Coccus, bar bacterium, Li Site bacterium and lactobacillus have inhibitory action, also have obvious antibacterial activity to bacterial spore；Sorbic acid
Potassium is internationally recognized low toxicity, efficient acid type preservative, can effectively suppress the work of mycete, yeast and aerobic bacteria
Property, thus reach effectively to extend the holding time of food, and keep the local flavor of original food；Sodium alginate low heat value, nontoxic
Property, easily expand, suppleness high, there is good thickening property, stability, gelation, foam stability and promote the effects such as cohesion,
Sodium alginate is dissolved in hot water and cold water forms sticky shape colloid solution, is the hydrophilic gel that a kind of hydratability is the strongest,
To the water holding of aquatic products is fresh-keeping, there is good effect, it addition, it or a kind of dietary fiber, there is reduction cholesterol, prevention
The absorption of the metal ions such as harmful element strontium cadmium, treatment hypertension, coronary heart disease and the health-care effect of diabetes；Lactic acid bacteria has good
Good biological antagonist activity, has the breeding of suppression putrefaction bacteria, clears up the antibacterial activities such as the toxin that putrefaction bacteria produces, be also improved
The antioxidant activity such as SOD enzyme activity, elimination human free radical, lactic acid bacteria is applied to biological antiseptic, is possible not only to suppress pathogenic bacterium
With the growth of putrefaction bacteria, and under normal cooling condition of storage, nor affect on the organoleptic attribute of food；Propolis is to adopt through Apis
Collection processing a kind of natural, safe colloidal solid material, containing multiple bioactive composition, have antibacterial, antioxidation and
The functions such as food fresh keeping, can significantly inhibit aquatic products bacterial number, highlight aquatic products antiseptic effect so that it is during preservation by low temperature
Between significantly extend.Compounding and the dosage optimization of multiple antibacterial component, can reduce production cost, can significantly widen again antimicrobial spectrum,
Multiple-microorganism in aquatic products decay process and enzyme are played and comprehensively suppresses and kill effect.
The present invention further preferably spice extract, spice extract by the Rhizoma Alpiniae Officinarum of certain part by weight proportioning, Bulbus Allii,
Bulbus Allii Cepae, Cortex cinnamomi japonici (Ramulus Cinnamomi), Flos Caryophylli, Herba Rosmarini Officinalis use ethanol immersion to extract.Wherein Rhizoma Alpiniae Officinarum is rich in flavonoid, volatile oil, Phenylpropanoid Glycosides
Class, diaryl heptane class, glucosides class active substance, can eliminate free radical, anti-lipid peroxidation, avoid lipid peroxidation to machine
The antioxidant activity of the damage of body, in addition, Rhizoma Alpiniae Officinarum also has the effects such as antibacterial, antiviral, antitumor, blood sugar lowering；Greatly
Bulbus Allii, rich in garlicin, has broad spectrum antibiotic activity, is referred to as " natural broad ectrum antibiotic ", and garlicin can also suppress parasite raw
Long, there is stronger antiviral activity；Rich in phytocide in Bulbus Allii Cepae, there is the strongest antibacterial antisepsis；Cortex cinnamomi japonici (Ramulus Cinnamomi) rich in
Oleum Cinnamomi, is mainly composed of cinnamic aldehyde and a small amount of acetic acid Cortex cinnamomi japonici (Ramulus Cinnamomi) ester, phenylpropyl acetate etc., has aobvious to antibacterial, mycete and yeast
The inhibitory action write；The effective ingredient of Flos Caryophylli extract is Oleum Caryophylli, mainly contains eugenol, acetyleugenol, β-stone
The compositions such as bamboo alkene, methyl salicylate, chavicol, have antibacterial, anthelmintic, the effect such as stomach invigorating, fungus is killed effect it is further preferred that；Fan
The most fragrant extract, rich in materials such as terpenoid, flavone, phenols, is a kind of singlet oxygen inhibitor, has preferable anti-oxidation characteristics.
Spice extract can also give food with local flavor, makes flavour of food products coordinate, and strengthens typical case's local flavor, plays and help digest and inhale
The effect received.
The multiple crude antistaling agent with different antibacterial feature is compounded by the present invention according to fence theory, and constantly adjusts
Try its collocation kind and concentration, a kind of composite preservative being exclusively used in preservation of fishery of final acquisition.Protect for aquatic products
Fresh test finds, the aquatic products composite preservative contaminating microorganisms multiple to aquatic products of the present invention has potent resistance of wide spectrum,
Rotten microorganism such as antibacterial, fungus, virus, parasite etc. are respectively provided with strong suppression and kill effect in cause common to aquatic products, protect
Fresh effective, can be greatly prolonged the shelf life of cold-storage water product, and pure natural of the present invention, safety non-toxic, abundance, one-tenth
This is suitable, is suitable for extensive Industry Promotion.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described in detail, it should be appreciated that embodiment is served only for this is described
Bright rather than be used for limiting the invention, any made on the basis of the present invention amendment, equivalent etc. is all at this
In bright protection domain.
Embodiment 1
Formula preparation aquatic products composite preservative according to following:
Containing following components in every liter of distilled water: lysozyme 0.5g, tea polyphenols 3g, carboxymethyl chitosan 18g, nisin
12g, potassium sorbate 30g, sodium alginate 20g, lactic acid bacteria 25g, propolis extract 2g, spice extract 12g.
The raw material of spice extract be weight ratio be the Rhizoma Alpiniae Officinarum of 5-7:4-6:3-5:2-3:4-6:4-5, Bulbus Allii, ocean
Herba Alii fistulosi, Cortex cinnamomi japonici (Ramulus Cinnamomi), Flos Caryophylli, Herba Rosmarini Officinalis spice combinations of substances.
Propolis extract, spice extract extracting method the most identical, be not provided with comparative test.
Embodiment 2
Formula preparation aquatic products composite preservative (lysozyme adds the preferred of concentration) according to following:
Containing following components in every liter of distilled water: the addition of lysozyme arranges the gradient of 0.1g, 0.2g, 0.3g, 0.4g, 0.6g
Test, tea polyphenols 3g, carboxymethyl chitosan 18g, nisin 12g, potassium sorbate 30g, sodium alginate 20g, lactic acid bacteria
25g, propolis extract 2g, spice extract 12g.
The raw material of spice extract be weight ratio be the Rhizoma Alpiniae Officinarum of 5-7:4-6:3-5:2-3:4-6:4-5, Bulbus Allii, ocean
Herba Alii fistulosi, Cortex cinnamomi japonici (Ramulus Cinnamomi), Flos Caryophylli, Herba Rosmarini Officinalis spice combinations of substances.
Embodiment 3
Formula preparation aquatic products composite preservative (tea polyphenols adds the preferred of concentration) according to following:
Containing following components in every liter of distilled water: lysozyme 0.5g, the addition of tea polyphenols arranges the gradient examination of 1g, 2g, 4g, 5g
Testing, carboxymethyl chitosan 18g, nisin 12g, potassium sorbate 30g, sodium alginate 20g, lactic acid bacteria 25g, propolis extracts
Thing 2g, spice extract 12g.
The raw material of spice extract be weight ratio be the Rhizoma Alpiniae Officinarum of 5-7:4-6:3-5:2-3:4-6:4-5, Bulbus Allii, ocean
Herba Alii fistulosi, Cortex cinnamomi japonici (Ramulus Cinnamomi), Flos Caryophylli, Herba Rosmarini Officinalis spice combinations of substances.
Embodiment 4
Formula preparation aquatic products composite preservative (carboxymethyl chitosan adds the preferred of concentration) according to following:
Containing following components in every liter of distilled water: lysozyme 0.5g, tea polyphenols 3g, the addition of carboxymethyl chitosan arrange 6g,
The gradient test of 12g, 24g, 30g, nisin 12g, potassium sorbate 30g, sodium alginate 20g, lactic acid bacteria 25g, propolis
Extract 2g, spice extract 12g.
The raw material of spice extract be weight ratio be the Rhizoma Alpiniae Officinarum of 5-7:4-6:3-5:2-3:4-6:4-5, Bulbus Allii, ocean
Herba Alii fistulosi, Cortex cinnamomi japonici (Ramulus Cinnamomi), Flos Caryophylli, Herba Rosmarini Officinalis spice combinations of substances.
Embodiment 5
Formula preparation aquatic products composite preservative (nisin adds the preferred of concentration) according to following:
Containing following components in every liter of distilled water: lysozyme 0.5g, tea polyphenols 3g, carboxymethyl chitosan 18g, nisin
Addition the gradient test of 4g, 8g, 16g, 20g is set, potassium sorbate 30g, sodium alginate 20g, lactic acid bacteria 25g, propolis carries
Take thing 2g, spice extract 12g.
The raw material of spice extract be weight ratio be the Rhizoma Alpiniae Officinarum of 5-7:4-6:3-5:2-3:4-6:4-5, Bulbus Allii, ocean
Herba Alii fistulosi, Cortex cinnamomi japonici (Ramulus Cinnamomi), Flos Caryophylli, Herba Rosmarini Officinalis spice combinations of substances.
Embodiment 6
Formula preparation aquatic products composite preservative (sodium alginate adds the preferred of concentration) according to following:
Containing following components in every liter of distilled water: lysozyme 0.5g, tea polyphenols 3g, carboxymethyl chitosan 18g, nisin
12g, potassium sorbate 30g, the addition of sodium alginate arranges the gradient test of 5g, 10g, 15g, 25g, 30g, lactic acid bacteria 25g, honeybee
Glue extract 2g, spice extract 12g.
The raw material of spice extract be weight ratio be the Rhizoma Alpiniae Officinarum of 5-7:4-6:3-5:2-3:4-6:4-5, Bulbus Allii, ocean
Herba Alii fistulosi, Cortex cinnamomi japonici (Ramulus Cinnamomi), Flos Caryophylli, Herba Rosmarini Officinalis spice combinations of substances.
Embodiment 7
Formula preparation aquatic products composite preservative (lactic acid bacteria adds the preferred of concentration) according to following:
Containing following components in every liter of distilled water: lysozyme 0.5g, tea polyphenols 3g, carboxymethyl chitosan 18g, nisin
12g, potassium sorbate 30g, sodium alginate 20g, the addition of lactic acid bacteria arranges the gradient test of 5g, 10g, 15g, 20g, 30g, honeybee
Glue extract 2g, spice extract 12g.
The raw material of spice extract be weight ratio be the Rhizoma Alpiniae Officinarum of 5-7:4-6:3-5:2-3:4-6:4-5, Bulbus Allii, ocean
Herba Alii fistulosi, Cortex cinnamomi japonici (Ramulus Cinnamomi), Flos Caryophylli, Herba Rosmarini Officinalis spice combinations of substances.
Embodiment 8
Formula preparation aquatic products composite preservative (propolis extract adds the preferred of concentration) according to following:
Containing following components in every liter of distilled water: lysozyme 0.5g, tea polyphenols 3g, carboxymethyl chitosan 18g, nisin
12g, potassium sorbate 30g, sodium alginate 20g, lactic acid bacteria 25g, the addition of propolis extract arranges the gradient examination of 1g, 3g, 4g
Test, spice extract 12g.
The raw material of spice extract be weight ratio be the Rhizoma Alpiniae Officinarum of 5-7:4-6:3-5:2-3:4-6:4-5, Bulbus Allii, ocean
Herba Alii fistulosi, Cortex cinnamomi japonici (Ramulus Cinnamomi), Flos Caryophylli, Herba Rosmarini Officinalis spice combinations of substances.
Embodiment 9
Formula preparation aquatic products composite preservative (spice extract adds the preferred of concentration) according to following:
Containing following components in every liter of distilled water: lysozyme 0.5g, tea polyphenols 3g, carboxymethyl chitosan 18g, nisin
12g, potassium sorbate 30g, sodium alginate 20g, lactic acid bacteria 25g, propolis extract 2g, the addition of spice extract is arranged
The gradient test of 4g, 8g, 16g, 20g.
The raw material of spice extract be weight ratio be the Rhizoma Alpiniae Officinarum of 5-7:4-6:3-5:2-3:4-6:4-5, Bulbus Allii, ocean
Herba Alii fistulosi, Cortex cinnamomi japonici (Ramulus Cinnamomi), Flos Caryophylli, Herba Rosmarini Officinalis spice combinations of substances.
Embodiment 10
Formula preparation aquatic products composite preservative (spice extract combination preferred) according to following:
Containing following components in every liter of distilled water: lysozyme 0.5g, tea polyphenols 3g, carboxymethyl chitosan 18g, nisin
12g, potassium sorbate 30g, sodium alginate 20g, lactic acid bacteria 25g, propolis extract 2g, spice extract 12g.
The raw material of spice extract be weight ratio be the Bulbus Allii of 4-6:3-5:2-3:4-6:4-5, Bulbus Allii Cepae, Cortex cinnamomi japonici (Ramulus Cinnamomi), fourth
Fragrant, Herba Rosmarini Officinalis spice combinations of substances.
Embodiment 11
Formula preparation aquatic products composite preservative (spice extract combination preferred) according to following:
Containing following components in every liter of distilled water: lysozyme 0.5g, tea polyphenols 3g, carboxymethyl chitosan 18g, nisin
12g, potassium sorbate 30g, sodium alginate 20g, lactic acid bacteria 25g, propolis extract 2g, spice extract 12g.
The raw material of spice extract be weight ratio be the Rhizoma Alpiniae Officinarum of 5-7:3-5:2-3:4-6:4-5, Bulbus Allii Cepae, Cortex cinnamomi japonici (Ramulus Cinnamomi),
Flos Caryophylli, Herba Rosmarini Officinalis spice combinations of substances.
Embodiment 12
Formula preparation aquatic products composite preservative (spice extract combination preferred) according to following:
Containing following components in every liter of distilled water: lysozyme 0.5g, tea polyphenols 3g, carboxymethyl chitosan 18g, nisin
12g, potassium sorbate 30g, sodium alginate 20g, lactic acid bacteria 25g, propolis extract 2g, spice extract 12g.
The raw material of spice extract be weight ratio be the Rhizoma Alpiniae Officinarum of 5-7:4-6:2-3:4-6:4-5, Bulbus Allii, Cortex cinnamomi japonici (Ramulus Cinnamomi),
Flos Caryophylli, Herba Rosmarini Officinalis spice combinations of substances.
Embodiment 13
Formula preparation aquatic products composite preservative (spice extract combination preferred) according to following:
Containing following components in every liter of distilled water: lysozyme 0.5g, tea polyphenols 3g, carboxymethyl chitosan 18g, nisin
12g, potassium sorbate 30g, sodium alginate 20g, lactic acid bacteria 25g, propolis extract 2g, spice extract 12g.
The raw material of spice extract be weight ratio be the Rhizoma Alpiniae Officinarum of 5-7:4-6:3-5:2-3:4-6:4-5, Bulbus Allii, ocean
Herba Alii fistulosi, Flos Caryophylli, Herba Rosmarini Officinalis spice combinations of substances.
Embodiment 14
Formula preparation aquatic products composite preservative (spice extract combination preferred) according to following:
Containing following components in every liter of distilled water: lysozyme 0.5g, tea polyphenols 3g, carboxymethyl chitosan 18g, nisin
12g, potassium sorbate 30g, sodium alginate 20g, lactic acid bacteria 25g, propolis extract 2g, spice extract 12g.
The raw material of spice extract be weight ratio be the Rhizoma Alpiniae Officinarum of 5-7:4-6:3-5:2-3:4-5, Bulbus Allii, Bulbus Allii Cepae,
Cortex cinnamomi japonici (Ramulus Cinnamomi), Herba Rosmarini Officinalis spice combinations of substances.
Embodiment 15
Formula preparation aquatic products composite preservative (spice extract combination preferred) according to following:
Containing following components in every liter of distilled water: lysozyme 0.5g, tea polyphenols 3g, carboxymethyl chitosan 18g, nisin
12g, potassium sorbate 30g, sodium alginate 20g, lactic acid bacteria 25g, propolis extract 2g, spice extract 12g.
The raw material of spice extract be weight ratio be the Rhizoma Alpiniae Officinarum of 5-7:4-6:3-5:2-3:4-6, Bulbus Allii, Bulbus Allii Cepae,
Cortex cinnamomi japonici (Ramulus Cinnamomi), the spice combinations of substances of Flos Caryophylli.
On June 18th, 2015, fish for company limited's buying tuna and oriental prawn from Lianyungang safety deep-sea fishing,
Select bright without the tuna block of muscle congestion and oriental prawn, transport laboratory under bubble chamber heat-retaining condition back real immediately
Test.
Experimental technique: tuna and oriental prawn are placed under 10 DEG C of air natural defrosting 3h, then tuna are cut into
Fritter, takes equal in quality tuna block and each random packet of oriental prawn, tuna block and oriental prawn examination in embodiment 1-15
Test each gradient test of group and two repetitions are respectively set, tuna block and oriental prawn are placed in the different formulations of embodiment 1-15
Aquatic product fresh keeping agent in respectively impregnate 2.5min, pull after draining under the conditions of 0 DEG C loading valve bag out, and in 0 DEG C of storage, 15 days
Rear mensuration tuna block and the total plate count of oriental prawn respectively.Total plate count assay method is with reference to " GB47892-2010 food
Microbiological Test total plate count measures ", observe by the naked eye colonial morphology, observe in conjunction with microscope and differentiate bacterium colony kind, knot
Fruit is such as following table 1-9.
From table 1-4 and table 6-8 it can be seen that use the aquatic products composite preservative of the present invention to aquatic products tuna and east
Side prawn fresh-keeping, preferable to the suppression of microorganism and killing effect, after 15 days, the quantity of microorganism is relatively low and kind is more miscellaneous,
Reflect that the initial contaminating bacteria entrained by aquatic products is the most significantly bred.The aquatic products composite preservative of the present invention is than it
Its any contrast test all significantly suppress the breeding of microorganism, and reducing certain component all can affect antistaling agent and press down microorganism
System or killing effect, and increase one-component and can not significantly improve the fresh-keeping effectiveness of antistaling agent, and the increase of constituent content can carry
The production cost of high-preserved dose.Therefore, by the test of table 1-4 and table 6-8 it is found that the aquatic products composite fresh-keeping of the present invention
Agent is the optimum combination combining production cost and fresh-keeping effect.
As can be found from Table 5, sodium alginate primarily serves effect of water conservation water holding in the present invention, increases alginic acid equally
The addition of sodium can not significantly improve Water-saving effect, makes antistaling agent excessively thickness on the contrary, it has not been convenient to uses, but also increases life
Produce cost.
By the spice extract of table 9 combine preferably it is found that in the aquatic products composite preservative of the present invention
Spice extract can preferably make up the short slab to individual microbial killing effect of the antistaling agent composition in addition, finally
Play for aquatic products contaminating microorganisms and putrefaction bacteria and comprehensive splendid kill effect.
Embodiment 16
In order to the antistaling agent that relatively aquatic products composite preservative provided by the present invention and forefathers use is to preservation of fishery effect
Difference, we find three kinds of aquatic products composite fresh-keeping agent prescriptions from technical literature, after preparing, have cooked antistaling agent simultaneously
Fresh-keeping effect contrast test.Three kinds of antistaling agent prescriptions are as follows with source:
Antistaling agent prescription: (documents derives from the " tea of Xiong Qing etc. to 8g/L tea polyphenols+5g/L citric acid+2g/LL-cysteine
The impact on cold preservation Penaeus vannamei quality of the polyphenol composite preservative ")；
Antistaling agent prescription: (documents derives from Wu Xue to 0.2% tea polyphenols+1.5% carboxymethyl chitosan+0.3% propolis extract
" complex biological preservative effect to scallop cold storing and fresh-keeping " of beautiful grade)；
Antistaling agent prescription: (documents derives from the shell of blue luxuriant green grass or young crops etc. to 10g/L chitosan+10g/L lysozyme+30g/L tea polyphenols
The polysaccharide complex biological preservative proportion optimizing to cold preservation Trichiurus haumela fresh-keeping effect ").
Choosing aquatic products tuna and oriental prawn equally as fresh-keeping test material, operational approach, investigation method are with real
Execute example 1-15, result such as table 10 below.
From the point of view of the fresh-keeping effect of different antistaling agents, aquatic products composite preservative provided by the present invention uses than forefathers
To the fresh-keeping effect of aquatic products more preferably, and the aquatic products composite preservative of the present invention is to use bio-preservative to compound to antistaling agent
Form, safety non-toxic, preparation, application process are simple, and suppression microorganism effect highlights, and good refreshing effect can be greatly prolonged cold
Hide the shelf life of aquatic products, there is important industrial value.
Those skilled in the art can be according to present disclosure and the art technology grasped in the present invention
Holding and make replacement or modification, but these are replaced or modification is all not regarded as a departure from present inventive concept, these are replaced or modification
All in claimed interest field.
Claims (6)
1. an aquatic products composite preservative, it is characterised in that in described every liter of distilled water of aquatic products composite preservative containing with
Lower component: lysozyme 0.4-0.6g, tea polyphenols 2.5-3.5g, carboxymethyl chitosan 16-20g, nisin 10-14g, mountain
Potassium sorbate 25-35g, sodium alginate 17-23g, lactic acid bacteria 20-30g, propolis extract 1.5-2.5g, spice extract 10-
14g。
Aquatic products composite preservative the most according to claim 1, it is characterised in that described aquatic products composite preservative is optimal
Formula is containing following components in every liter of distilled water: lysozyme 0.5g, tea polyphenols 3g, carboxymethyl chitosan 18g, streptococcus acidi lactici
Element 12g, potassium sorbate 30g, sodium alginate 20g, lactic acid bacteria 25g, propolis extract 2g, spice extract 12g.
3. according to the aquatic products composite preservative described in claim 1 and 2, it is characterised in that the extraction of described propolis extract
Method is: by propolis freezing and pulverizing remove impurity, with supercritical extraction instrument supercritical CO2Extraction, extracting pressure controls to be 30-35MPa,
Extraction temperature controls as 35-40 DEG C, CO2Flow-control is 16-22L/h, and extraction time controls as 3.5-4.5h, knockout tower discharging
Obtain the propolis extract of yellow paste.
4. according to the aquatic products composite preservative described in claim 1 and 2, it is characterised in that described spice extract former
Material be weight ratio be the Rhizoma Alpiniae Officinarum of 5-7:4-6:3-5:2-3:4-6:4-5, Bulbus Allii, Bulbus Allii Cepae, Cortex cinnamomi japonici (Ramulus Cinnamomi), Flos Caryophylli, Herba Rosmarini Officinalis perfume (or spice)
Pungent material combinations of substances.
5. according to the aquatic products composite preservative described in claim 1,2 and 4, it is characterised in that described spice extract
Extracting method is: being put into by spice in air dry oven and dry, high-speed tissue mashing machine adds 95% ethanol, feed liquid after smashing to pieces
Than controlling to shake 12h for 1g:10ml, 100r/min constant-temperature table, with filter paper filtering, residue is pressed solid-liquid ratio 1g:4ml and is added 95%
Ethanol, crosses after bringing up again 12h and filters filtering residue, is merged by twice extracting solution, is placed in decompression distillation in Rotary Evaporators, obtains spice
Extract.
Aquatic products composite preservative the most according to claim 5, it is characterised in that described spice is dried, constant-temperature table
The temperature that concussion, Rotary Evaporators decompression are distilled is all less than 50 DEG C.
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