CN107318949A - Tuna specific complex antistaling agent - Google Patents
Tuna specific complex antistaling agent Download PDFInfo
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- CN107318949A CN107318949A CN201710544882.1A CN201710544882A CN107318949A CN 107318949 A CN107318949 A CN 107318949A CN 201710544882 A CN201710544882 A CN 201710544882A CN 107318949 A CN107318949 A CN 107318949A
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- 229910052700 potassium Inorganic materials 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 230000009182 swimming Effects 0.000 description 1
- 235000019166 vitamin D Nutrition 0.000 description 1
- 239000011710 vitamin D Substances 0.000 description 1
- 150000003710 vitamin D derivatives Chemical class 0.000 description 1
- 229940046008 vitamin d Drugs 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B4/00—General methods for preserving meat, sausages, fish or fish products
- A23B4/14—Preserving with chemicals not covered by groups A23B4/02 or A23B4/12
- A23B4/18—Preserving with chemicals not covered by groups A23B4/02 or A23B4/12 in the form of liquids or solids
- A23B4/20—Organic compounds; Microorganisms; Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B4/00—General methods for preserving meat, sausages, fish or fish products
- A23B4/14—Preserving with chemicals not covered by groups A23B4/02 or A23B4/12
- A23B4/18—Preserving with chemicals not covered by groups A23B4/02 or A23B4/12 in the form of liquids or solids
- A23B4/20—Organic compounds; Microorganisms; Enzymes
- A23B4/22—Microorganisms; Enzymes; Antibiotics
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/461—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from fish
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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Abstract
The invention discloses tuna specific complex antistaling agent, it is constituted and its parts by weight are：8 10 parts of Tea Polyphenols, 18 20 parts of chitosan, 0.8 1 parts of ε polylysines, 0.8 1 parts of nisin, 0.8 1 parts of active oligopeptide, 35 parts of biology enzyme, 8 10 parts of organic acid mixture, 8 10 parts of plant extraction liquid, 900 1000 parts of distilled water, active oligopeptide is obtained using composite protease hydrolysis tuna albumen, active oligopeptide is purified with ammonium sulfate precipitation method with centrifuging.Have the beneficial effect that：The inventive method raw materials materials are natural, inexpensive environmental protection, finished product has superpower biocidal property, inoxidizability, and the color and luster and freshness of tuna are not influenceed, possesses permanent fresh-keeping timeliness, freshness date by a couple of days of common antistaling agent can be extended to 50 70 days, be it is a kind of it is efficient, nontoxic, safely, the natural tuna specific complex antistaling agent that has no side effect.
Description
Technical field
The present invention relates to antistaling agent field, more particularly, to a kind of tuna specific complex antistaling agent.
Technical background
Tuna, also known as yaito tuna, tuna fish, are mainly distributed on the Mid-low latitude abysmal area such as the Pacific Ocean, the Indian Ocean, Atlantic Ocean
Domain, is a kind of large-scale pelagic important goods food fish, sees world Nuan Shui marine sites, and the nearly edge such as mackerel, chub mackerel, Spanish mackerel, generally same
Li Qing sections.The yellowish pink of tuna is red, because caused by containing substantial amounts of myoglobins in the muscle of tuna.Some
Tuna, such as bluefin tuna can utilize the metabolism for flesh of swimming；Make the temperature of internal blood higher than extraneous water temperature.This life
Reason function makes tuna can adapt to larger water temperature range, so as to survive in the relatively low waters of temperature.Tuna is long-term
Free of contamination abysmal area is moved in, is considered as green, safe and pollution-free food, with very high nutritive value, thus it is international
Nutritive issue is recommended as one of big nutrition fish in the world three.Its nutritional ingredient include egg from matter, lipid, carbohydrate, calcium, phosphorus, iron,
Sodium, potassium, magnesium, zinc, copper, VitAVitE, vitamin D, cholesterol etc..Compared with general fish and meat, tuna tool
Have low fat, low in calories and high protein the characteristics of.
Tuna is easily putrid and deteriorated article, and corruption is easier compared with general food, so research tuna
Antistaling agent play the role of to the economic development for improving Tuna Fisheries it is huge, current tuna is fresh-keeping mainly have chilled method,
Preservation by partial, freezing process etc..And bio-preservative is harmful to human health, what is had also has certain nutritive value, is from now on
Study the Main way of antistaling agent.But due to causing the putrid and deteriorated bacterial species of the flesh of fish various, distinct, single kind of anti-corrosion
The bacteriostasis property of antistaling agent is limited, hardly results in preferable fresh-keeping effect.
At present, the preservation technology on tuna has many methods, and prior art is as authorized public number to be CN
103053672 B Chinese invention patent, discloses a kind of tuna preservation method, and tuna is first put in soak by this method
In, be placed in be mixed with the binary ice of the materials such as foaming agent, surfactant after then pulling out and stored, with make it is simple,
Easily operated advantage, but it understands the harmful substances such as remaining surface activating agent and foaming agent, and its preservation liquid only has
Lyase bacterium, therefore its fresh-keeping effect is limited, without promotional value.
The content of the invention
It is an object of the invention to provide a kind of fresh keeping time is long, nontoxic secondary material residual tuna specific complex is fresh-keeping
Agent.
The present invention is directed to the problem of being mentioned in background technology, and the technical scheme taken is：Tuna specific complex antistaling agent,
It is constituted and its parts by weight are：8-10 parts of Tea Polyphenols, 18-20 parts of chitosan, 0.8-1 parts of epsilon-polylysine, nisin
0.8-1 parts, 0.8-1 parts of active oligopeptide, 3-5 parts of biology enzyme, 8-10 parts of organic acid mixture, 8-10 parts of plant extraction liquid, distillation
900-1000 parts of water, the tuna specific complex antistaling agent has superpower biocidal property, inoxidizability, and the color of tuna is not influenceed
Pool and freshness, possess permanent fresh-keeping timeliness, and are a kind of natural, efficiently, safe golden rifles without malicious secondary material residual
Fish specific complex antistaling agent.
Preferably, tuna specific complex antistaling agent, its preparation method includes：
Enzymolysis：Weigh fresh tuna 100-120g mixing 150-200g distilled water stirring to crush to oppress slurry, inject close
Close in rustless steel container, it is 5-7 to adjust its pH with 0.1mol/L hydrochloric acid, 0.07-0.1g compound protease is added, in 50-
Reacted 2.5-3 hours at 60 DEG C, after be warming up to 90-100 DEG C of boiling and inactivate enzyme in 5-8 minutes, after be down to normal temperature and obtain enzymolysis liquid,
Tuna protein zymolyte contains a kind of active oligopeptide under the conditions of being somebody's turn to do, and the active oligopeptide can be by disturbing bacterial cell membrane just
Chang Gongneng, causes infiltration, nutrient loss and the film potential of cell membrane to decline, makes aqtocytolysis to cause pathogenic bacteria and spoilage organisms
The death of cell, the amino acid sequence of the active oligopeptide is：SCASRCKSRCRARRCGYYVRGRCYCRCLRC；
Thick purification：Enzymolysis liquid is placed in a centrifuge, 10-15 is centrifuged under the conditions of 4000-4500r/min in 35-38 DEG C of temperature
Minute, take supernatant；By supernatant at 4-6 DEG C refrigerated centrifuge, the lower centrifugation 10-15 under the conditions of rotating speed 7500-8000r/min
Minute, take supernatant；Finely ground ammonium sulfate is added in above-mentioned supernatant according to 55-60% saturation degree, preserved at 4-6 DEG C
Centrifuged 30-45 minutes, the distilled water for taking precipitation to add 2-3 times stirs to obtain crude extract, applied with 9500-10000r/min after overnight
Ammonium sulfate purification can effectively increase the accounting of active oligopeptide, be prepared to be further purified；
Purifying：Gel chromatography is carried out to crude extract from glucose gel, it is first that glucose gel is small with distilled water immersion 45-48
When it is fully swelled and remove floating gel particle, using known molecular amount for 3.354 kDa standard items lactobacillus peptide and
5.8 kDa insulin obtains the relation of elution time and molecular weight, by pretreated crude extract upper prop, collects molecular weight
For 3-5kDa component, by the active oligopeptide solution of purifying in vacuum 0.1-0.2kPa, temperature is progress under the conditions of 65-70 DEG C
Drying is concentrated in vacuo, active oligopeptide solid is produced, by purifying, the content of active oligopeptide is greatly improved, and not only increases whole production
The fresh-keeping effect of thing antistaling agent, moreover it is possible to cost-effective and work efficiency；
It is compound：Weigh 8-10 parts of Tea Polyphenols, 18-20 parts of chitosan, 0.8-1 parts of epsilon-polylysine, nisin 0.8-1
Part, 0.8-1 parts of active oligopeptide, 3-5 parts of biology enzyme, 8-10 parts of organic acid, 8-10 parts of plant extraction liquid, distilled water 900-1000
Part, produce within uniform stirring 15-20 minutes at a temperature of 30-35 DEG C composite preservative, natural, the cheap ring of this composite preservative materials
Protect, freshness date by a couple of days of common antistaling agent can be extended to 50-70 days, and composite preservative easily divides after entrance human body
Solution, has no toxic side effect to human body, be it is a kind of it is efficient, nontoxic, safely, the natural tuna specific complex antistaling agent that has no side effect.
Preferably, biology enzyme is：Low-temperature protease or lysozyme or glucose oxidase or isoamylase or cellulose
Enzyme.
Preferably, organic acid is：Caffeic acid or tannic acid and MAP.
Preferably, plant extraction liquid is：Acupuncture needle mushroom extracting solution or wild marjoram extract solution or corn essential oil.
Compared with prior art, the advantage of the invention is that：Natural, the inexpensive environmental protection of materials, active monster and nisin
Both derivatives of rhzomorph can be broken down into amino acid after human body is entered and be absorbed and used, therefore without the secondary material of any poison
Residual；With superpower biocidal property, inoxidizability, the color and luster and freshness of tuna are not influenceed, possess permanent fresh-keeping timeliness,
Freshness date by a couple of days of common antistaling agent can be extended to 50-70 days, be it is a kind of it is efficient, nontoxic, safely, the day that has no side effect
Right tuna specific complex antistaling agent.
Embodiment
The present invention program is described further below by embodiment：
Embodiment 1：
Tuna specific complex antistaling agent, it is constituted and its parts by weight are：Weigh 8 parts of Tea Polyphenols, 18 parts of chitosan, ε-poly- bad ammonia
0.8 part of acid, 0.8 part of nisin, 0.8 part of active oligopeptide, 1 part of lysozyme, 2 parts of isoamylase, 4 parts of caffeic acid, tannic acid 4
Part, 4 parts of acupuncture needle mushroom extracting solution, 4 parts of corn essential oil, 900 parts of distilled water, its preparation comprises the following steps：
1）Weigh fresh tuna 100g mixing 150g distilled water stirring to crush to oppress slurry, inject closed rustless steel container
In, it is 5 that its pH is adjusted with 0.1mol/L hydrochloric acid, adds 0.07g compound protease, is reacted 2.5 hours at 50 DEG C, rear to rise
Temperature inactivates enzyme in 5 minutes to 90 DEG C of boilings, after be down to normal temperature and obtain enzymolysis liquid, tuna protein zymolyte contains one under this condition
Kind of active oligopeptide, the active oligopeptide can cause infiltration, the nutrient flow of cell membrane by disturbing the normal function of bacterial cell membrane
Film potential of becoming estranged declines, and makes aqtocytolysis to cause the death of pathogenic bacteria and spoilage organisms cell；2）Enzymolysis liquid is placed in centrifugation
In machine, centrifuged in 35 DEG C of temperature under the conditions of 4000r/min 10 minutes, take supernatant；By supernatant at 4 DEG C refrigerated centrifuge,
Lower centrifugation 10 minutes, take supernatant under the conditions of rotating speed 7500r/min；Finely ground ammonium sulfate is added according to 55% saturation degree
Into above-mentioned supernatant, centrifuged 30 minutes with 9500r/min after being preserved overnight at 4 DEG C, the distilled water stirring for taking precipitation to add 2 times
Crude extract is obtained, can effectively increase the accounting of active oligopeptide using ammonium sulfate purification, be prepared to be further purified；3）By grape
Sugared gel is made it fully be swelled for 45 hours and is removed floating gel particle with distilled water immersion, is come using lactobacillus peptide and insulin
Elution time and the relation of molecular weight are obtained, by pretreated crude extract upper prop, the component that molecular weight is 3-5kDa is collected, will
The active oligopeptide solution of purifying is in vacuum 0.1kPa, and temperature is progress vacuum concentration drying under the conditions of 65 DEG C, produces active widow
Peptide solid, by purifying, the content of active oligopeptide is greatly improved, and not only increases the fresh-keeping effect of end-product antistaling agent, moreover it is possible to
Cost-effective and work efficiency；4）Weigh 8 parts of Tea Polyphenols, 18 parts of chitosan, 0.8 part of epsilon-polylysine, 0.8 part of nisin,
0.8 part of active oligopeptide, 1 part of lysozyme, 2 parts of isoamylase, 4 parts of caffeic acid, 4 parts of tannic acid, 4 parts of acupuncture needle mushroom extracting solution, precision corn
Oily 4 parts, 900 parts of distilled water, uniform stirring produces composite preservative, this composite preservative materials day for 15 minutes at a temperature of 30 DEG C
So, cheap environmental protection, by a couple of days of common antistaling agent can extend to 50-70 days by freshness date, and composite preservative is in entrance
Easily decomposed after human body, human body had no toxic side effect, be it is a kind of it is efficient, nontoxic, safely, the natural tuna that has no side effect it is special multiple
Close antistaling agent.
Embodiment 2：
Tuna specific complex antistaling agent, it is constituted and its parts by weight are：10 parts of Tea Polyphenols, 20 parts of chitosan, epsilon-polylysine 1
Part, 1 part of nisin, 1 part of active oligopeptide, 2 parts of low-temperature protease, 2 parts of glucose oxidase, 1 part of cellulase, coffee
10 parts of acid, 5 parts of wild marjoram extract solution, 5 parts of corn essential oil, 1000 parts of distilled water, its preparation comprises the following steps：
1）Weigh fresh tuna 120g mixing 200g distilled water stirring to crush to oppress slurry, inject closed rustless steel container
In, it is 7 that its pH is adjusted with 0.1mol/L hydrochloric acid, adds 0.1g compound protease, is reacted 3 hours at 60 DEG C, rear heating
Inactivate enzyme within 8 minutes to 100 DEG C of boilings, after be down to normal temperature and obtain enzymolysis liquid, tuna protein zymolyte contains one under this condition
Kind of active oligopeptide, the active oligopeptide can cause infiltration, the nutrient flow of cell membrane by disturbing the normal function of bacterial cell membrane
Film potential of becoming estranged declines, and makes aqtocytolysis to cause the death of pathogenic bacteria and spoilage organisms cell；
2）Enzymolysis liquid is placed in a centrifuge, is centrifuged in 38 DEG C of temperature under the conditions of 4500r/min 15 minutes, takes supernatant；Will
Supernatant refrigerated centrifuge at 6 DEG C, lower centrifugation 15 minutes, take supernatant under the conditions of rotating speed 8000r/min；By finely ground sulfuric acid
Ammonium is added in above-mentioned supernatant according to 560% saturation degree, is centrifuged 45 minutes, taken with 10000r/min after being preserved overnight at 6 DEG C
The distilled water that precipitation adds 3 times stirs to obtain crude extract, can effectively increase the accounting of active oligopeptide using ammonium sulfate purification, to enter
The purifying of one step is prepared；
3）Glucose gel is made it fully be swelled for 48 hours and removed floating gel particle with distilled water immersion, using lactic acid bacteria
Peptide and insulin obtain the relation of elution time and molecular weight, and by pretreated crude extract upper prop, collection molecular weight is 3-
5kDa component, by the active oligopeptide solution of purifying in vacuum 0.2kPa, temperature is dry to carry out being concentrated in vacuo under the conditions of 70 DEG C
It is dry, active oligopeptide solid is produced, by purifying, the content of active oligopeptide is greatly improved, and not only increases end-product antistaling agent
Fresh-keeping effect, moreover it is possible to cost-effective and work efficiency；
4）Weigh 10 parts of Tea Polyphenols, it is 20 parts of chitosan, 1 part of epsilon-polylysine, 1 part of nisin, 1 part of active oligopeptide, low
Warm 2 parts of protease, 2 parts of glucose oxidase, 1 part of cellulase, 10 parts of caffeic acid, 5 parts of wild marjoram extract solution, 5 parts of corn essential oil,
1000 parts of distilled water, produces composite preservative, this composite preservative materials for uniform stirring 15-20 minutes at a temperature of 30-35 DEG C
Naturally, cheap environmental protection, by a couple of days of common antistaling agent can extend to 50-70 days by freshness date, and composite preservative is entering
Enter and easily decomposed after human body, human body is had no toxic side effect, be it is a kind of it is efficient, nontoxic, safely, the natural tuna that has no side effect it is special
Composite preservative.
Embodiment 3：
Tuna specific complex antistaling agent, it is constituted and its parts by weight are：10 parts of Tea Polyphenols, 20 parts of chitosan, epsilon-polylysine 1
Part, 1 part of nisin, 1 part of active oligopeptide, 2 parts of lysozyme, 2 parts of cellulase, 3 parts of caffeic acid, 5 parts of tannic acid, asparagus
3 parts of extract solution, 5 parts of wild marjoram extract solution, 1000 parts of distilled water, its preparation comprises the following steps：
Enzymolysis：Weigh fresh tuna 100g mixing 150g distilled water stirring to crush to oppress slurry, inject closed stainless steel
In container, it is 6 that its pH is adjusted with 0.1mol/L hydrochloric acid, adds 0.07g compound protease, is reacted 2.5 hours at 50 DEG C,
After be warming up to 95 DEG C of boilings and inactivate enzyme in 5 minutes, after be down to normal temperature and obtain enzymolysis liquid, tuna protein zymolyte contains under this condition
There is a kind of active oligopeptide, the active oligopeptide can cause permeating, supporting for cell membrane by disturbing the normal function of bacterial cell membrane
Film potential decline of becoming estranged is shunted, makes aqtocytolysis to cause the death of pathogenic bacteria and spoilage organisms cell；
Thick purification：Enzymolysis liquid is placed in a centrifuge, is centrifuged in 35 DEG C of temperature under the conditions of 4000r/min 10 minutes, takes supernatant
Liquid；By supernatant at 4 DEG C refrigerated centrifuge, lower centrifugation 10 minutes, take supernatant under the conditions of rotating speed 7500r/min；Will be finely ground
Ammonium sulfate be added to according to 60% saturation degree in above-mentioned supernatant, at 4 DEG C preserve overnight after with 10000r/min centrifuge 30 points
Clock, the distilled water for taking precipitation to add 3 times stirs to obtain crude extract, and accounting for for active oligopeptide can be effectively increased using ammonium sulfate purification
Than being prepared to be further purified；
Purifying：Gel chromatography is carried out to crude extract from glucose gel, first by glucose gel distilled water immersion 48 hours
It is fully swelled and remove floating gel particle, using the standard items lactobacillus peptide and 5.8 that known molecular amount is 3.354 kDa
KDa insulin obtains the relation of elution time and molecular weight, by pretreated crude extract upper prop, and collection molecular weight is 3-
5kDa component, by the active oligopeptide solution of purifying in vacuum 0.2kPa, temperature is dry to carry out being concentrated in vacuo under the conditions of 70 DEG C
It is dry, active oligopeptide solid is produced, by purifying, the content of active oligopeptide is greatly improved, and not only increases end-product antistaling agent
Fresh-keeping effect, moreover it is possible to cost-effective and work efficiency；
It is compound：Weigh 10 parts of Tea Polyphenols, 20 parts of chitosan, 1 part of epsilon-polylysine, 1 part of nisin, 1 part of active oligopeptide,
2 parts of lysozyme, 2 parts of cellulase, 3 parts of caffeic acid, 5 parts of tannic acid, 3 parts of acupuncture needle mushroom extracting solution, 5 parts of wild marjoram extract solution, distilled water
1000 parts, composite preservative is produced within uniform stirring 15-20 minutes at a temperature of 30-35 DEG C, this composite preservative materials are natural, honest and clean
Valency environmental protection, by a couple of days of common antistaling agent can extend to 50-70 days by freshness date, and composite preservative is after entrance human body
Easily decompose, human body had no toxic side effect, be it is a kind of it is efficient, nontoxic, safely, the natural tuna specific complex that has no side effect it is fresh-keeping
Agent.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail embodiment described above, it should be understood that it is described above only
For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should be included in the scope of the protection.
SEQUENCE LISTING
<110>Pujiang County Ou Li Bioisystech Co., Ltd
<120>Tuna specific complex antistaling agent
<130> 5
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 30
<212> PRT
<213>It is artificial synthesized
<400> 1
Ser Cys Ala Ser Arg Cys Lys Ser Arg Cys Arg Ala Arg Arg Cys Gly
1 5 10 15
Tyr Tyr Val Arg Gly Arg Cys Tyr Cys Arg Cys Leu Arg Cys
20 25 30
Claims (10)
1. tuna specific complex antistaling agent, it is characterised in that：The composition and its parts by weight of described composite preservative be：Tea is more
8-10 parts of phenol, 18-20 parts of chitosan, 0.8-1 parts of epsilon-polylysine, 0.8-1 parts of nisin, 0.8-1 parts of active oligopeptide,
3-5 parts of biology enzyme, 8-10 parts of organic acid, 8-10 parts of plant extraction liquid, 900-1000 parts of distilled water.
2. tuna specific complex antistaling agent according to claim 1, it is characterised in that：Described biology enzyme is：Low temperature
Protease or lysozyme or glucose oxidase or isoamylase or cellulase.
3. tuna specific complex antistaling agent according to claim 1, it is characterised in that：Described organic acid is：Coffee
Acid or tannic acid or MAP.
4. tuna specific complex antistaling agent according to claim 1, it is characterised in that：Described plant extraction liquid is：
Acupuncture needle mushroom extracting solution or wild marjoram extract solution or corn essential oil.
5. tuna specific complex antistaling agent according to claim 1, it is characterised in that：The amino of described active oligopeptide
Acid sequence is：SCASRCKSRCRARRCGYYVRGRCYCRCLRC.
6. tuna specific complex antistaling agent according to claim 1, it is characterised in that：Described preparation method include with
Lower step：
1）Enzymolysis：Digest tuna protein and obtain active oligopeptide；
2）Thick purification：With centrifugation active oligopeptide is purified with ammonium sulfate precipitation；
3）Purifying：Active oligopeptide is further purified with glucose gel chromatography；
4）It is compound：Prepare composite preservative.
7. tuna specific complex antistaling agent according to claim 6, it is characterised in that：Described enzymolysis step is：Press
According to mass ratio 1:1.5-2 weighs fresh tuna and crushes to oppress slurry with distilled water stirring, injects closed rustless steel container
In, it is 5-7 to adjust its pH with 0.1mol/L hydrochloric acid, adds 0.07-0.1g compound protease, is reacted at 50-60 DEG C
2.5-3 hours, after be warming up to 90-100 DEG C of boiling and inactivate enzyme in 5-8 minutes, after be down to normal temperature and obtain enzymolysis liquid.
8. tuna specific complex antistaling agent according to claim 6, it is characterised in that：Described thick purification step is：
Enzymolysis liquid is placed in a centrifuge, is centrifuged in 35-38 DEG C of temperature under the conditions of 4000-4500r/min 10-15 minutes, takes supernatant
Liquid；By supernatant at 4-6 DEG C refrigerated centrifuge, lower centrifugation 10-15 minutes, take supernatant under the conditions of rotating speed 7500-8000r/min
Liquid；Finely ground ammonium sulfate is added in above-mentioned supernatant according to 55-60% saturation degree, used after being preserved overnight at 4-6 DEG C
9500-10000r/min is centrifuged 30-45 minutes, and the distilled water for taking precipitation to add 2-3 times stirs to obtain crude extract.
9. tuna specific complex antistaling agent according to claim 6, it is characterised in that：Distilled in described purification step
The time of water logging pickled grape sugar gel is 45-48 hours, and the vacuum for being concentrated in vacuo dried active oligopeptides is 0.1-0.2kPa, temperature
Spend for 65-70 DEG C.
10. tuna specific complex antistaling agent according to claim 6, it is characterised in that：Described composite steps are：Claim
Take 8-10 parts of Tea Polyphenols, 18-20 parts of chitosan, 0.8-1 parts of epsilon-polylysine, 0.8-1 parts of nisin, active oligopeptide
0.8-1 parts, 3-5 parts of biology enzyme, 8-10 parts of organic acid, 8-10 parts of plant extraction liquid, 900-1000 parts of distilled water, at 30-35 DEG C
At a temperature of uniform stirring produce composite preservative within 15-20 minutes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710544882.1A CN107318949A (en) | 2017-07-06 | 2017-07-06 | Tuna specific complex antistaling agent |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710544882.1A CN107318949A (en) | 2017-07-06 | 2017-07-06 | Tuna specific complex antistaling agent |
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| Publication Number | Publication Date |
|---|---|
| CN107318949A true CN107318949A (en) | 2017-11-07 |
Family
ID=60195812
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710544882.1A Pending CN107318949A (en) | 2017-07-06 | 2017-07-06 | Tuna specific complex antistaling agent |
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| CN (1) | CN107318949A (en) |
Cited By (6)
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| CN108077392A (en) * | 2017-12-04 | 2018-05-29 | 浙江海洋大学 | A kind of tuna freeze preservation method |
| CN108719441A (en) * | 2018-05-09 | 2018-11-02 | 河南科技学院 | A kind of complex biological preservative and its application for broiler carcass bacteria reducing processing |
| CN109122841A (en) * | 2018-08-04 | 2019-01-04 | 天津科技大学 | The color protecting method of gadus polypeptide anti-enzymatic regent and potato fresh slices |
| CN109479948A (en) * | 2018-12-10 | 2019-03-19 | 湖北楚玉莱信克食品科技有限公司 | A kind of single-freeze fillets of ictalurus punctatus processing method for keeping fresh |
| CN113575881A (en) * | 2021-08-03 | 2021-11-02 | 佛山市顺德万汇美食工业研究有限公司 | Method for making raw fish |
| CN115707381A (en) * | 2022-10-25 | 2023-02-21 | 武汉轻工大学 | Corn bait fresh-keeping composition, fresh-keeping method and fresh-keeping corn bait |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108077392A (en) * | 2017-12-04 | 2018-05-29 | 浙江海洋大学 | A kind of tuna freeze preservation method |
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| CN109122841A (en) * | 2018-08-04 | 2019-01-04 | 天津科技大学 | The color protecting method of gadus polypeptide anti-enzymatic regent and potato fresh slices |
| CN109479948A (en) * | 2018-12-10 | 2019-03-19 | 湖北楚玉莱信克食品科技有限公司 | A kind of single-freeze fillets of ictalurus punctatus processing method for keeping fresh |
| CN113575881A (en) * | 2021-08-03 | 2021-11-02 | 佛山市顺德万汇美食工业研究有限公司 | Method for making raw fish |
| CN113575881B (en) * | 2021-08-03 | 2023-08-04 | 佛山市顺德万汇美食工业研究有限公司 | Method for making raw fish |
| CN115707381A (en) * | 2022-10-25 | 2023-02-21 | 武汉轻工大学 | Corn bait fresh-keeping composition, fresh-keeping method and fresh-keeping corn bait |
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