CN105062888B - Bar magnet pipe sleeve, multi-joint component and instrument for extracting nucleic acid - Google Patents
Bar magnet pipe sleeve, multi-joint component and instrument for extracting nucleic acid Download PDFInfo
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- CN105062888B CN105062888B CN201510556307.4A CN201510556307A CN105062888B CN 105062888 B CN105062888 B CN 105062888B CN 201510556307 A CN201510556307 A CN 201510556307A CN 105062888 B CN105062888 B CN 105062888B
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/02—Magnetic separation acting directly on the substance being separated
- B03C1/28—Magnetic plugs and dipsticks
- B03C1/284—Magnetic plugs and dipsticks with associated cleaning means, e.g. retractable non-magnetic sleeve
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/02—Magnetic separation acting directly on the substance being separated
- B03C1/025—High gradient magnetic separators
- B03C1/031—Component parts; Auxiliary operations
- B03C1/033—Component parts; Auxiliary operations characterised by the magnetic circuit
- B03C1/0332—Component parts; Auxiliary operations characterised by the magnetic circuit using permanent magnets
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/02—Magnetic separation acting directly on the substance being separated
- B03C1/28—Magnetic plugs and dipsticks
- B03C1/286—Magnetic plugs and dipsticks disposed at the inner circumference of a recipient, e.g. magnetic drain bolt
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C2201/00—Details of magnetic or electrostatic separation
- B03C2201/18—Magnetic separation whereby the particles are suspended in a liquid
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C2201/00—Details of magnetic or electrostatic separation
- B03C2201/26—Details of magnetic or electrostatic separation for use in medical applications
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Abstract
The present invention relates to a kind of bar magnet pipe sleeve, multi-joint component and instrument for extracting nucleic acid, instrument for extracting nucleic acid, including multi-joint component, fixing device and the actuating unit being connected with fixing device for driving the rotation of bar magnet pipe sleeve, the bar magnet pipe sleeve on multi-joint component is driven to be rotated at a high speed relative to supporting plate by actuating unit, the rotary speed of bar magnet pipe sleeve can be adjusted by the speed of motivation of adjustment mechanism, can meet the requirement of high viscosity magnetic bead flowing；By driving the rotation at a high speed of bar magnet pipe sleeve so that stirring structure high-speed stirred reagent solution, the phenomenon of reagent solution splashing is eliminated, so that it is wide to mix region, magnetic bead absorption, washing, elution are abundant, and nucleic acid yield is greatly improved, while also reducing the production cost to instrument for extracting nucleic acid.
Description
Technical field
The present invention relates to nucleic acid extraction technical field, more particularly to a kind of bar magnet pipe sleeve, multi-joint component and nucleic acid extraction
Instrument.
Background technology
Extract thin in multiple fields such as modern molecular biology research, clinical disease diagnosis, blood transfusion, food safety detections
Born of the same parents, and the bioactive substance such as protein, nucleic acid play an important roll.Wherein, nucleic acid by many nucleotide polymerizations into life
Active substances compound, is one of most basic material of life.Nucleic acid is widely present in all animals, plant cell, micro- life
In thing, in organism.Different according to chemical composition, nucleic acid can be divided into ribonucleic acid, abbreviation RNA and DNA, referred to as
DNA.The main matter basis that DNA is storage, replicates and conveys hereditary information, RNA plays important in protein building-up process
Effect.The development of biotechnology is maked rapid progress, with PCR (Polymerase Chain Reaction, polymerase chain reaction) skill
Art is in the application of every field, including disease detection, agricultural aspect detection GMOs and other are very many in terms of medical science
Using etc., it is necessary to high-throughout sample bioactive substance extract and full-automatic bioactive substance extract method and instrument.
But in its tangible actual application, often because in conventional sample much impurity mix with DNA, influence, disturb, even suppressing
DNA, RNA course of reaction so that experimental result is complicated.
To sample cell, DNA, RNA, the extraction of albumen and the pure processing of purifying, so that its product is tested for all kinds of downstreams
Interpretation of result, is a key link for solving round pcr practical application.The classical way of purification has：Boiling lysis, phenol chlorine
Imitative extraction method, silicon fiml absorption method, Beads enrichment method etc..Wherein, Beads enrichment method utilization being capable of specific recognition cell, protein
Or the magnetic bead of nucleic acid extracts bioactive substance.Magnetic bead can specifically be combined with nucleic acid molecules, then in the effect in magnetic field
Under, separated from the active macromolecules in blood, animal tissue, food, pathogenic microorganism equal samples.Beads enrichment method is that have
Four basic process links are constituted：(1) crack：Discharge the DNA in sample；(2) adsorb：Adjust specific be adsorbed onto outside magnetic bead
On coated pellosil；(3) wash：The magnet that magnetic bead is isolated outside sleeve pipe is fixed, and the removal of impurity is gone in washing；(4) elute：By changing
Become the conditions such as pH value, chaotropic salt to elute nucleic acid from the pellosil of magnetic bead expoeridium.
Beads enrichment method automatic extracting instrument is divided into bar magnet method and suction method.Suction method is entered by automatic pipetting device
OK.By taking paramagnetic particle method nucleic acid purification instrument as an example, the absorption of cracking process magnetic bead can be added by automatic pipetting device；Take solution away, plus
Enter rinsing liquid, magnetic bead absorption takes rinsing liquid away, adds elution buffer.Due to not taken out when suction method, which exists, removes waste liquid
Magnetic bead is walked, liquid-transfering device can not lean on magnetic bead too near, the problem of being taken out to prevent magnetic bead together with waste liquid, influence elution efficiency and pure
Degree.
Bar magnet method extraction method is the technology that developed recently gets up, and its principle is that magnetic separation equipment is immersed into liquid,
The particle that can be attracted magnetically in mixture is set to concentrate on separation equipment surface by magnetic fields.In one way in which, band
The separation equipment of magnetic is a sleeve pipe, makes sleeve pipe produce magnetic by inserting bar magnet, attracts to be attracted magnetically in mixture
Granular absorption to sleeve surface, when bar magnet is detached, sleeve pipe loses magnetism, and the particle that is attracted magnetically departs from sleeve pipe.Band
The mechanism of dynamic bar magnet can drive bar magnet to be moved up and down in sleeve pipe, contribute to the cracking, rinsing and purifying of extraction process.
But it is dynamic to simulate reagent mixing that nucleic acid purification instrument in the market all covers the OQ t of isolation using bar magnet TIP
Make, but can all occur mixing insufficient phenomenon, so that causing Beads enrichment to test nucleic acid yield can not actually approach
100%.
The content of the invention
Based on this, it is necessary to for above-mentioned nucleic acid extraction yield it is low the problem of there is provided a kind of bar magnet pipe sleeve.
A kind of bar magnet pipe sleeve, including side wall and bottom plate, the side wall and bottom plate surround one have opening be used for accommodate
Also including for stirring the stirring structure of reagent solution and for dynamic with instrument for extracting nucleic acid on the cavity of bar magnet, the bar magnet pipe sleeve
The docking structure of force mechanisms docking, the stirring structure is on the side wall and/or bottom plate, and the docking structure is located at described
On the wall of side.
In one of the embodiments, the stirring structure is on the outer surface of the side wall and/or bottom plate.
In one of the embodiments, the stirring structure including being on the bottom plate and/or wall outer surface
The projection of sheet.
In one of the embodiments, offered on one end relative with bottom plate on the side wall for supporting the bar magnet
The extension of pipe sleeve.
In one of the embodiments, the extension is the convex portion for protruding from the wall outer surface.
In one of the embodiments, the docking structure includes the butt hole matched with actuating unit, the docking
Hole is opened on the extension.
In one of the embodiments, the bottom plate is made up or the bottom plate in conical or described bottom plate of hydrophobic material
Hydrophobic coating is coated on outer surface.
Above-mentioned bar magnet pipe sleeve, rotation at a high speed is realized by the actuating unit of docking structure and instrument for extracting nucleic acid to fetching,
Reagent solution is stirred by stirring structure while rotation, the phenomenon of reagent solution splashing is eliminated so that region is mixed extensively, magnetic bead is inhaled
Attached, washing, elution are abundant, and nucleic acid yield is greatly improved, while also reducing the production cost to instrument for extracting nucleic acid.
For above-mentioned nucleic acid extraction yield it is low the problem of, a kind of multi-joint component is also provided.
A kind of multi-joint component, including supporting plate and several above-mentioned bar magnet pipe sleeves, the supporting plate are sheathed on several
On the bar magnet pipe sleeve, the bar magnet pipe sleeve can be rotated against with the supporting plate.
In one of the embodiments, the through hole passed through for the bar magnet pipe sleeve is offered in the supporting plate and institute is available for
The tooth mouthful that stirring structure passes through is stated, the through hole is communicated with the tooth mouthful.
Above-mentioned multi-joint component, including supporting plate and bar magnet pipe sleeve, supporting plate are used to support bar magnet pipe sleeve, and bar magnet pipe sleeve is dynamic
Relative support plate rotates at a high speed under the drive of force mechanisms.Bar magnet pipe sleeve stirs reagent solution while rotation by stirring structure,
Eliminate the phenomenon of reagent solution splashing so that mix region extensively, magnetic bead absorption, washing, elution are abundant, and nucleic acid yield obtains very big
Lifting, while also reducing the production cost to instrument for extracting nucleic acid.
For above-mentioned nucleic acid extraction yield it is low the problem of, a kind of instrument for extracting nucleic acid is also provided.
A kind of multi-joint component and the drive bar magnet pipe sleeve including fixing device, above-mentioned rotates relative to the supporting plate
The actuating unit being fixedly connected with the fixing device.
Above-mentioned instrument for extracting nucleic acid, including multi-joint component, fixing device and drive connecting with fixing device for bar magnet pipe sleeve rotation
The actuating unit connect, drives the bar magnet pipe sleeve on multi-joint component to be rotated at a high speed relative to supporting plate, bar magnet pipe by actuating unit
The rotary speed of set can be adjusted by the speed of motivation of adjustment mechanism, can meet the requirement of high viscosity magnetic bead flowing；It is logical
Cross the rotation at a high speed of drive bar magnet pipe sleeve so that stirring structure high-speed stirred reagent solution, eliminate the phenomenon of reagent solution splashing so that
Mix region wide, magnetic bead absorption, washing, elution are abundant, and nucleic acid yield is greatly improved, while also reducing to nucleic acid extraction
The production cost of instrument.
Brief description of the drawings
Fig. 1 is the structural representation of present pre-ferred embodiments bar magnet pipe sleeve；
Fig. 2 is present pre-ferred embodiments bar magnet sleeve pipe structure diagrammatic top view；
Fig. 3 is present pre-ferred embodiments bar magnet sleeve pipe structure schematic isometric；
Fig. 4 is the supporting plate structure schematic diagram of the multi-joint component of present pre-ferred embodiments；
Fig. 5 is the structural representation of the multi-joint component of present pre-ferred embodiments；
Fig. 6 is present pre-ferred embodiments instrument for extracting nucleic acid part body schematic diagram.
Embodiment
Fig. 1~Fig. 3 is refer to, present pre-ferred embodiments provide a kind of bar magnet pipe sleeve 100.Bar magnet pipe sleeve 100 includes side
Wall 110 and bottom plate 120.Side wall 110 surrounds a cavity 111 for being used to accommodate bar magnet with opening with bottom plate 120.Bar magnet pipe
Also include what is be used for the stirring structure 130 for stirring reagent solution and docked for the actuating unit 310 with instrument for extracting nucleic acid on set 100
Docking structure.Stirring structure 130 is on side wall 110 and/or bottom plate 120.Docking structure is on side wall 110.The bar magnet pipe
Set 100 coordinates bar magnet, relative by the actuating unit 310 of docking structure and instrument for extracting nucleic acid, so that in the drive of dynamic structure
It is lower to rotate, reagent solution is stirred by stirring structure 130, so as to reach the effect of Beads enrichment.
Side wall 110 is around the formation cavity 111 of bottom plate 120, at the opening of cavity 111, i.e., with the phase of bottom plate 120 on side wall 110
To one end on offer extension 150.When carrying out Beads enrichment experiment using the bar magnet pipe sleeve 100, pass through the extension
150 are engaged with supporting plate 210, can position the bar magnet pipe sleeve 100, make the bar magnet pipe sleeve 100 can be with normal work.Specifically
, the extension 150 is the convex portion for protruding from the outer surface of side wall 110.So, bar magnet pipe sleeve 100 is directly passed through into supporting plate 210
During placement, you can realize the positioning in supporting plate 210, such positioning method is simple in construction.The convex portion is in outside side wall 110
Can be in an annulus on surface, i.e. convex portion is arranged in the whole circumference of side wall 110；Also may be used on the outer surface of side wall 110 convex portion
With in scattered projection.Projection at least two, and each projection is respectively positioned on same cross section, so just be can guarantee that and is worked as bar magnet
It is stable that pipe sleeve 100, which is positioned at the position positioned when in supporting plate 210,.
Open up position of the docking structure on bar magnet pipe sleeve 100 is not specifically limited, and in the present embodiment, docking structure is located at
On extension 150.Docking structure includes the butt hole matched with the actuating unit 310 on instrument for extracting nucleic acid.That is the butt hole
Match with actuating unit 310, when actuating unit 310 works, pass through the cooperation of actuating unit 310 and butt hole, engine
Structure 310 can drive bar magnet pipe sleeve 100 to operate.In other embodiments, it can also pass through between actuating unit 310 and docking structure
Other modes are docked, and such as side tooth, fastener, are not specifically limited herein, as long as can realize that drive bar magnet pipe sleeve 100 rotates
Function.
Stirring structure 130 is on the outer surface of the side wall 110 of bar magnet pipe sleeve 100 and/or bottom plate 120, projection in the form of sheets.
When bar magnet pipe sleeve 100 is rotated, the stirring structure 130 on side wall 110 and the outer surface of bottom plate 120 can be stirred reagent solution
Mix, and the projection that stirring structure 130 is set into sheet can be during bar magnet pipe sleeve 100 be rotated so that stirring is more square
Just, uniformly, similar quant is stirred continuously in water.
Bottom plate 120 is designed as the profile of easy water conservancy diversion, such as taper, curved surface or opens up guiding gutter, so, when bar magnet pipe sleeve
When 100 rotations stop, the reagent solution for remaining in the outer surface of bar magnet pipe sleeve 100 (outer surface of side wall 110 and bottom plate 120) can be suitable
Outer surface to flow back into Reagent Tube.Hydrophobic coating or bottom plate 120 can certainly be coated on the outer surface of bottom plate 120
Body is just made up of hydrophobic material.It is such to can be designed so that reagent solution adhered on bottom plate 120 etc. smoothly be flow back into
In Reagent Tube.
Referring to Fig. 4~Fig. 5, a preferred embodiment of the invention also provides a kind of multi-joint component 200.This is multi-joint
Component 200 includes bar magnet pipe sleeve 100 and supporting plate 210.Supporting plate 210 is sheathed on several identical bar magnet pipe sleeves 100, and
Each bar magnet pipe sleeve 100 can be rotated against with supporting plate 210.Bar magnet pipe sleeve 100 is above-mentioned bar magnet pipe sleeve 100, and its is specific
Architectural feature is not repeated herein.
The through hole 211 passed through for bar magnet pipe sleeve 100 is offered in supporting plate 210.Because bar magnet pipe sleeve 100 is provided with stirring
Structure 130, tooth mouthful 212 is further opened with order that obtaining stirring structure 130 and can pass through in through hole 211, supporting plate 210.Tooth
Mouth 212 is communicated with through hole 211.Specifically, the number of tooth mouthful 212 is specifically to open up position, to enable to bar magnet pipe sleeve 100
Through hole 211 is passed through to be defined.When bar magnet pipe sleeve 100 passes through the through hole 211 in supporting plate 210, stirring structure 130 is aligned
Tooth mouthful 212, you can ensure that bar magnet pipe sleeve 100 can pass through through hole 211.
The extension 150 set on bar magnet pipe sleeve 100 is so that supporting plate 210 is sheathed on bar magnet pipe sleeve 100 without going out
Now depart from, that is, realize and bar magnet pipe sleeve 100 is positioned in supporting plate 210.
Referring to Fig. 6, a preferred embodiment of the invention also provides a kind of instrument for extracting nucleic acid.The instrument for extracting nucleic acid
Including the bar magnet pipe sleeve 100 on fixing device, bar magnet bar 320, multi-joint component 200 and the multi-joint component 200 of drive relative to support
The actuating unit 310 being fixedly connected with fixing device that plate 210 rotates.Multi-joint component 200 is above-mentioned multi-joint component 200, and its is right
Here is omitted for the structure answered.
Supporting plate 210 is fixedly connected in fixing device.Bar magnet pipe sleeve 100 is sheathed in supporting plate 210.Actuating unit
310 are connected with bar magnet pipe sleeve 100, and bar magnet pipe sleeve 100 can be driven to be rotated relative to supporting plate 210.Bar magnet is placed in the bar magnet
In pipe sleeve 100, and it can be moved up and down in bar magnet pipe sleeve 100.
Specifically, actuating unit 310 drives bar magnet pipe sleeve 100 not done relative to the kind of drive that supporting plate 210 rotates and had
Body is limited.Actuating unit 310 is motor.The motor shaft of motor directly can be relatively fixed with the docking structure on bar magnet pipe sleeve 100
Connection, so when motor works, motor shaft can drive bar magnet pipe sleeve 100 to rotate.Because motor shaft can rotate at a high speed, bar magnet
Pipe sleeve 100 can also rotate at a high speed under the drive of motor shaft.By on bar magnet pipe sleeve 100 in the rotary course of bar magnet pipe sleeve 100
Stirring structure 130 stirs reagent solution, substantially increases nucleic acid yield.
Certainly, in other embodiments, transmission mechanism 330 can also be increased between motor and bar magnet pipe sleeve 100.Transmission
Mechanism 330 includes the transmission belt being connected with motor shaft and the rotating cylinder being connected with transmission belt.Rotating cylinder is sheathed on outside bar magnet, by electricity
Arbor drives rotation.And the rotation of rotating cylinder has no effect on moving up and down for bar magnet bar 320.Rotating cylinder is gone back and docking structure simultaneously
It is connected, when being rotated under the drive of motor shaft, drives bar magnet pipe sleeve 100 to rotate.For transmission shape of the motor to bar magnet pipe sleeve 100
Formula is not specifically limited herein, as long as transmission can be realized.
Due to being provided with several bar magnet pipe sleeves 100 in multi-joint component 200, and each bar magnet pipe sleeve 100 can be in engine
Relative support plate 210 is rotated under the drive of structure 310.Separate it can be rotated between each bar magnet pipe sleeve 100, also can be mutual
Transmission rotation.When each 100 separate rotation of bar magnet pipe sleeve, each bar magnet pipe sleeve 100 is connected with actuating unit 310,
Directly rotated by the drive of actuating unit 310., can be by wherein one when mutually transmission rotation between each bar magnet pipe sleeve 100
Individual bar magnet pipe sleeve 100 is connected with actuating unit 310, is directly rotated by the drive of actuating unit 310, it is bar magnet that remaining is remaining
Pipe sleeve 100 is connected two-by-two, and mutually transmission rotation, specific form is not specifically limited herein.
When carrying out nucleic acid extraction using instrument for extracting nucleic acid, it will first placed several bar magnet pipe sleeves 100 and be fixed to fixed fill
Put.Wherein, position of the number of specific bar magnet pipe sleeve 100 i.e. in supporting plate 210 on instrument for extracting nucleic acid with needing to carry
Take the Reagent Tube of nucleic acid corresponding.The Reagent Tube for extracting nucleic acid is each needed to correspond to a bar magnet pipe sleeve 100.Start nucleic acid
Extraction apparatus, bar magnet is moved downward to stopping in bar magnet pipe sleeve 100.Motor starts slow work, during slow work, with
Docking structure docking on bar magnet pipe sleeve 100.After mating operation is completed, motor starts rapid operation, to drive bar magnet pipe
Set 100 rotates at a high speed.After nucleic acid extraction is completed, instrument for extracting nucleic acid is closed, motor is stopped, bar magnet removes bar magnet pipe sleeve
100。
Above-mentioned instrument for extracting nucleic acid, the bar magnet pipe sleeve 100 on multi-joint component 200 is driven relative to branch by actuating unit 310
Fagging 210 is rotated, and the rotary speed of bar magnet pipe sleeve 100 can be adjusted by the speed of motivation of adjustment mechanism 310, can be met
The requirement of high viscosity magnetic bead flowing；By the rotation of bar magnet pipe sleeve 100, the phenomenon of reagent solution splashing is essentially eliminated, area is mixed
Domain is wide, and magnetic bead absorption, washing, elution are abundant, and nucleic acid yield is greatly improved, and tends to more than 99%, while also reducing pair
The production cost of instrument for extracting nucleic acid.
Each technical characteristic of embodiment described above can be combined arbitrarily, to make description succinct, not to above-mentioned reality
Apply all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, the scope of this specification record is all considered to be.
Embodiment described above only expresses the several embodiments of the present invention, and it describes more specific and detailed, but simultaneously
Can not therefore it be construed as limiting the scope of the patent.It should be pointed out that coming for one of ordinary skill in the art
Say, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection of the present invention
Scope.Therefore, the protection domain of patent of the present invention should be determined by the appended claims.
Claims (5)
1. a kind of bar magnet pipe sleeve, it is characterised in that including side wall and bottom plate, the side wall surrounds one with bottom plate has opening
Also include being used to stir the stirring structure of reagent solution and for carrying with nucleic acid on cavity for accommodating bar magnet, the bar magnet pipe sleeve
The docking structure for taking the actuating unit of instrument to dock, the stirring structure is on the side wall and/or bottom plate, the docking structure
On the side wall；
The extension for supporting the bar magnet pipe sleeve is offered on the side wall on one end relative with bottom plate；The extension
To protrude from the convex portion of the wall outer surface；
The stirring structure includes the projection in the form of sheets on the wall outer surface；
Open up jagged on the extension；The breach is correspondingly arranged with the stirring structure；
The docking structure includes the butt hole matched with actuating unit, and the butt hole is opened on the extension.
2. bar magnet pipe sleeve according to claim 1, it is characterised in that the bottom plate is in conical or described bottom plate by hydrophobic
Material be made or the plate outer surface on coat hydrophobic coating.
3. a kind of multi-joint component, it is characterised in that including supporting plate and several magnetic as described in any one of claim 1~2
Rod pipe sleeve, the supporting plate is sheathed on several described bar magnet pipe sleeves, and the bar magnet pipe sleeve can relative rotation with the supporting plate
Turn.
4. multi-joint component according to claim 3, it is characterised in that offered in the supporting plate for the bar magnet pipe sleeve
The through hole passed through and the tooth mouthful for being available for the stirring structure to pass through, the through hole are communicated with the tooth mouthful.
5. a kind of instrument for extracting nucleic acid, it is characterised in that multi-joint component and band including fixing device, as described in claim 3 or 4
Move the actuating unit being fixedly connected with the fixing device that the bar magnet pipe sleeve rotates relative to the supporting plate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510556307.4A CN105062888B (en) | 2015-09-02 | 2015-09-02 | Bar magnet pipe sleeve, multi-joint component and instrument for extracting nucleic acid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510556307.4A CN105062888B (en) | 2015-09-02 | 2015-09-02 | Bar magnet pipe sleeve, multi-joint component and instrument for extracting nucleic acid |
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| CN105062888A CN105062888A (en) | 2015-11-18 |
| CN105062888B true CN105062888B (en) | 2017-09-15 |
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Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105543090B (en) * | 2016-01-19 | 2018-08-10 | 山西振东基赛生物科技有限公司 | A kind of dehydration device and method |
| CN105420074B (en) * | 2016-01-19 | 2018-06-29 | 山西振东基赛生物科技有限公司 | A kind of multifunctional bio material disconnecting gear |
| KR20180090543A (en) * | 2017-02-03 | 2018-08-13 | 주식회사 미코바이오메드 | Apparatus and Process For Isolating or Purifying Target Material From Biological Sample |
| CN106906121B (en) * | 2017-04-17 | 2019-05-21 | 新疆出入境检验检疫局检验检疫技术中心 | The nucleic acid separator and separation method of magnet control |
| CN109207340B (en) * | 2017-06-30 | 2022-08-12 | 开启基因股份有限公司 | Nucleic acid extraction assembly |
| CN110042054A (en) * | 2018-01-16 | 2019-07-23 | 青岛益柏生物科技有限公司 | A kind of automation magnetic bead separating device |
| CN112823202B (en) * | 2018-08-24 | 2023-12-19 | 深圳华大生命科学研究院 | Cell and biomolecule separation and extraction device and test system |
| CN113234591B (en) * | 2021-05-28 | 2024-03-22 | 宁波康程德诺生物医药有限公司 | Integrated nucleic acid quick-lifting test tube, quick-lifting detection device and method |
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| KR100483684B1 (en) * | 2003-01-29 | 2005-04-18 | (주)바이오넥스 | Kit for separating and purifying nucleic acids or various biological materials, and system for automatically performing separation or purification of biological materials using the same |
| CN103756871B (en) * | 2013-12-30 | 2015-04-22 | 西安天隆科技有限公司 | Single sample nucleic acid enclosed extractor |
| CN203923174U (en) * | 2014-06-26 | 2014-11-05 | 广州冠科生物科技有限公司 | A kind of nucleic acid extraction evenly mixing device |
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