CN106172146A - A kind of artificial insemination method of rainbow trout - Google Patents
A kind of artificial insemination method of rainbow trout Download PDFInfo
- Publication number
- CN106172146A CN106172146A CN201610838487.XA CN201610838487A CN106172146A CN 106172146 A CN106172146 A CN 106172146A CN 201610838487 A CN201610838487 A CN 201610838487A CN 106172146 A CN106172146 A CN 106172146A
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- rainbow trout
- germ cell
- artificial insemination
- water
- ovum
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- 238000000034 method Methods 0.000 title claims abstract description 47
- 230000009027 insemination Effects 0.000 title claims abstract description 27
- 241001327682 Oncorhynchus mykiss irideus Species 0.000 title 1
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 38
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 38
- 210000004602 germ cell Anatomy 0.000 claims abstract description 37
- 241000277275 Oncorhynchus mykiss Species 0.000 claims abstract description 36
- 241000251468 Actinopterygii Species 0.000 claims abstract description 35
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 31
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 16
- 238000005213 imbibition Methods 0.000 claims abstract description 16
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 16
- 239000001301 oxygen Substances 0.000 claims abstract description 16
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 claims abstract 2
- 235000014103 egg white Nutrition 0.000 claims abstract 2
- 210000000969 egg white Anatomy 0.000 claims abstract 2
- 238000003756 stirring Methods 0.000 claims description 9
- LGZXYFMMLRYXLK-UHFFFAOYSA-N mercury(2+);sulfide Chemical compound [S-2].[Hg+2] LGZXYFMMLRYXLK-UHFFFAOYSA-N 0.000 claims description 8
- 238000002156 mixing Methods 0.000 claims description 2
- 241000277331 Salmonidae Species 0.000 claims 1
- 230000004720 fertilization Effects 0.000 abstract description 11
- 238000001816 cooling Methods 0.000 abstract description 3
- 239000013589 supplement Substances 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 210000004681 ovum Anatomy 0.000 description 36
- 229910052573 porcelain Inorganic materials 0.000 description 21
- 210000001015 abdomen Anatomy 0.000 description 14
- 230000012447 hatching Effects 0.000 description 14
- 210000000582 semen Anatomy 0.000 description 14
- 230000035800 maturation Effects 0.000 description 12
- 239000013505 freshwater Substances 0.000 description 11
- 241000543821 Oestrus Species 0.000 description 7
- 230000003187 abdominal effect Effects 0.000 description 7
- 230000012173 estrus Effects 0.000 description 7
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 208000028804 PERCHING syndrome Diseases 0.000 description 1
- 230000004596 appetite loss Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 238000005352 clarification Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 235000021266 loss of appetite Nutrition 0.000 description 1
- 208000019017 loss of appetite Diseases 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 235000020681 well water Nutrition 0.000 description 1
- 239000002349 well water Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses the artificial insemination method of a kind of rainbow trout, step includes that parent fish selects, dry method is inseminated, washed by egg white and imbibition, after mainly by inseminating dry method, using temperature is 9 15 DEG C, and dissolved oxygen amount is that germ cell is rinsed by the cold spring water of more than 7mg/L.Hinge structure of the present invention, fertilization water is made without extra cooling, oxygen supplement operation, can effectively improve rate of fertilization, it is possible to decrease manual labor's intensity and production cost, and the large-scale promotion for rainbow trout cultivation provides basis.
Description
Technical field
The present invention relates to the cultural technique field of freshwater fish culturing technical field, especially fresh water rainbow trout, be specifically related to one
Plant the artificial insemination method of rainbow trout.
Background technology
Rainbow trout, originates in the pacific cold water fishes in state, North America, is famous economic freshwater Fish, generally lives
In north cold area, south such as the high and cold mountain area of northern Guangxi, like perching cool in water quality clarification, the water yield is sufficient, have sand
In upstream, rivers and creeks, tributary or streams at the bottom of gravel, it is generally divided into fall sea, lake type, land envelope type three types.This fish growth water
Temperature: 8～20 DEG C, less than 8 DEG C or higher than 20 DEG C of loss of appetite, poor growth, optimum water temperature is: 12～18 DEG C, is fertilized, hatches
Process water temperature is less than 13 DEG C, and more than 24 DEG C dead.
Land envelope type rainbow trout at present, i.e. can be with natural fertilization under fresh water rainbow trout, natural conditions, can not under the conditions of artificial cultivation
Natural fertilization, it is necessary to carry out artificial insemination, current rainbow trout artificial insemination method has tended to ripe, but there are still some not
Foot, the insemination water as used mostly is tap water, well water or reservoir water, and dissolved oxygen amount is not up to the oxygen demand of rainbow trout insemination, needs
First oxygen supplement, cooling could use after processing, and need to use (NaCl, KCl, CaCl2) etc. the penetrating fluid of salt wash germ cell, shadow
Ring rate of fertilization, improve cost.
Summary of the invention
For solving above-mentioned technical problem, the invention provides the artificial insemination method of a kind of rainbow trout, by many with cold spring water
Secondary flushing germ cell, can effectively improve rate of fertilization.
The present invention is achieved by the following technical programs:
The artificial insemination method of a kind of rainbow trout, comprises the following steps:
1) parent fish selects
Select ripe female rainbow trout (average weight 1.5 kilograms) in 3 ages and ripe male fresh water rainbow trout (average body in 2 ages
Weigh 1.0 kilograms), start to adopt ovum, semen collection when parent fish oestruses.
2) dry method insemination
Using parent fish folder to be picked up by raun, dry moisture with gauze, the most parallel be placed on above porcelain basin, abdominal part is towards porcelain basin
Mouthful, use pressure abdomen method to squeeze in clean porcelain basin by female fish roe grain, every tail raun will obtain 1500-3500 a diameter of 4-not waited
The demersal egg of 7mm, picks up milter immediately, uses pressure abdomen method to be squeezed in basin on ovum grain by milter seminal fluid, and on band, rubber gloves is light
Light stirring, treats essence ovum mix homogeneously, and whole operation keeps room temperature≤20 DEG C.
3) germ cell is cleaned
After essence ovum mix homogeneously, being 9-15 DEG C by temperature, dissolved oxygen amount is that the cold spring water of more than 7mg/L rinses fertilization repeatedly
Ovum 5-8 time, until water becomes clear, the whole operating time is 3-5min.
4) imbibition
Stand 30-60min, allow the abundant imbibition of germ cell, choose remaining successful germ cell of being fertilized after the dead ovum of white
Go to hatching barrel hatching.
Further, step 1) parent fish select in female rainbow trout and the additional proportion of female rainbow trout be 1:1-3:1.
Further, step 2) described in dry method Process of Insemination in, control temperature at 10-15 DEG C.
Further, step 2) described in stirring, mixing speed is 50-60r/min.
Further, step 3) described in cold spring water, temperature is 10-12 DEG C, and dissolved oxygen amount is >=8mg/L.
Further, step 4) described in germ cell, successful germ cell of being fertilized is faint yellow or Chinese red.
The beneficial effects of the present invention is: after inseminating dry method, use temperature to be 9-15 DEG C, dissolved oxygen amount is 7mg/L
Germ cell is rinsed by above cold spring water, hinge structure of the present invention, fertilization water be made without extra cooling,
Oxygen supplement operates, and can effectively improve rate of fertilization, it is possible to decrease manual labor's intensity and production cost, for pushing away on a large scale of rainbow trout cultivation
Basis is extensively provided.
Detailed description of the invention
Below in conjunction with embodiment, technical scheme is further described, but claimed scope is not limited to
In described.
Embodiment 1
1) parent fish selects
Select female rainbow trout (average weight 1.5 kilograms) and the male fresh water rainbow trout of 6 tail 2 maturations in age of 6 tail 3 maturations in age
(average weight 1.0 kilograms), started to adopt ovum, semen collection when parent fish oestruses.
2) dry method insemination
Using parent fish folder to be picked up by raun, dry moisture with gauze, the most parallel be placed on above porcelain basin, abdominal part is towards porcelain basin
Mouthful, use pressure abdomen method to squeeze in clean porcelain basin by female fish roe grain, every tail raun will obtain 1500-3500 a diameter of 4-not waited
The demersal egg of 7mm, picks up milter immediately, uses pressure abdomen method to be squeezed in basin on ovum grain by milter seminal fluid, and on band, rubber gloves is light
Light stirring, treats essence ovum mix homogeneously, and whole operation keeps room temperature≤20 DEG C.
3) germ cell is cleaned
After essence ovum mix homogeneously, being 15 DEG C by temperature, dissolved oxygen amount is that the cold spring water of 7mg/L rinses germ cell 5 times repeatedly,
Until water becomes clear, the whole operating time is 3min.
4) imbibition
Standing 30min, allow the abundant imbibition of germ cell, after choosing the dead ovum of white, remaining successful germ cell of being fertilized is
For faint yellow or Chinese red, go to hatching barrel hatching.
Embodiment 2
1) parent fish selects
Select female rainbow trout (average weight 1.5 kilograms) and the male fresh water rainbow trout of 2 tail 2 maturations in age of 6 tail 3 maturations in age
(average weight 1.0 kilograms), started to adopt ovum, semen collection when parent fish oestruses.
2) dry method insemination
Using parent fish folder to be picked up by raun, dry moisture with gauze, the most parallel be placed on above porcelain basin, abdominal part is towards porcelain basin
Mouthful, use pressure abdomen method to squeeze in clean porcelain basin by female fish roe grain, every tail raun will obtain 1500-3500 a diameter of 4-not waited
The demersal egg of 7mm, picks up milter immediately, uses pressure abdomen method to be squeezed in basin on ovum grain by milter seminal fluid, and on band, rubber gloves is light
Light stirring, treats essence ovum mix homogeneously, and whole operation keeps room temperature≤20 DEG C.
3) germ cell is cleaned
After essence ovum mix homogeneously, being 9 DEG C by temperature, dissolved oxygen amount is that the cold spring water of 7mg/L rinses germ cell 8 times repeatedly,
Until water becomes clear, the whole operating time is 5min.
4) imbibition
Standing 60min, allow the abundant imbibition of germ cell, after choosing the dead ovum of white, remaining successful germ cell of being fertilized is
For faint yellow or Chinese red, go to hatching barrel hatching.
Embodiment 3
1) parent fish selects
Select female rainbow trout (average weight 1.5 kilograms) and the male fresh water rainbow trout of 4 tail 2 maturations in age of 6 tail 3 maturations in age
(average weight 1.0 kilograms), started to adopt ovum, semen collection when parent fish oestruses.
2) dry method insemination
Using parent fish folder to be picked up by raun, dry moisture with gauze, the most parallel be placed on above porcelain basin, abdominal part is towards porcelain basin
Mouthful, use pressure abdomen method to squeeze in clean porcelain basin by female fish roe grain, every tail raun will obtain 1500-3500 a diameter of 4-not waited
The demersal egg of 7mm, picks up milter immediately, uses pressure abdomen method to be squeezed in basin on ovum grain by milter seminal fluid, and on band, rubber gloves is light
Light stirring, treats essence ovum mix homogeneously, and whole operation keeps room temperature to carry out at 15 DEG C.
3) germ cell is cleaned
After essence ovum mix homogeneously, being 10 DEG C by temperature, dissolved oxygen amount is that the cold spring water of 8mg/L rinses germ cell 6 times repeatedly,
Until water becomes clear, the whole operating time is 4min.
4) imbibition
Standing 40min, allow the abundant imbibition of germ cell, after choosing the dead ovum of white, remaining successful germ cell of being fertilized is
For faint yellow or Chinese red, go to hatching barrel hatching.
Embodiment 4
1) parent fish selects
Select female rainbow trout (average weight 1.5 kilograms) and the male fresh water rainbow trout of 6 tail 5 maturations in age of 6 tail 3 maturations in age
(average weight 1.0 kilograms), started to adopt ovum, semen collection when parent fish oestruses.
2) dry method insemination
Using parent fish folder to be picked up by raun, dry moisture with gauze, the most parallel be placed on above porcelain basin, abdominal part is towards porcelain basin
Mouthful, use pressure abdomen method to squeeze in clean porcelain basin by female fish roe grain, every tail raun will obtain 1500-3500 a diameter of 4-not waited
The demersal egg of 7mm, picks up milter immediately, uses pressure abdomen method to be squeezed in basin on ovum grain by milter seminal fluid, and on band, rubber gloves is light
Light stirring, treats essence ovum mix homogeneously, and whole operation keeps room temperature to carry out at 10 DEG C.
3) germ cell is cleaned
After essence ovum mix homogeneously, being 12 DEG C by temperature, dissolved oxygen amount is that the cold spring water of more than 7mg/L rinses germ cell repeatedly
7 times, until water becomes clear, whole operation 5min.
4) imbibition
Standing 55min, allow the abundant imbibition of germ cell, after choosing the dead ovum of white, remaining successful germ cell of being fertilized is
For faint yellow or Chinese red, go to hatching barrel hatching.
Embodiment 5
1) parent fish selects
Select female rainbow trout (average weight 1.5 kilograms) and the male fresh water rainbow trout of 6 tail 6 maturations in age of 6 tail 3 maturations in age
(average weight 1.0 kilograms), started to adopt ovum, semen collection when parent fish oestruses.
2) dry method insemination
Using parent fish folder to be picked up by raun, dry moisture with gauze, the most parallel be placed on above porcelain basin, abdominal part is towards porcelain basin
Mouthful, use pressure abdomen method to squeeze in clean porcelain basin by female fish roe grain, every tail raun will obtain 1500-3500 a diameter of 4-not waited
The demersal egg of 7mm, picks up milter immediately, uses pressure abdomen method to be squeezed in basin on ovum grain by milter seminal fluid, and on band, rubber gloves is light
Light stirring, treats essence ovum mix homogeneously, and whole operation keeps room temperature≤20 DEG C.
3) germ cell is cleaned
After essence ovum mix homogeneously, being 12 DEG C by temperature, dissolved oxygen amount is that the cold spring water of more than 8mg/L rinses germ cell repeatedly
6 times, until water becomes clear, whole operation 4min.
4) imbibition
Standing 35min, allow the abundant imbibition of germ cell, after choosing the dead ovum of white, remaining successful germ cell of being fertilized is
For faint yellow or Chinese red, go to hatching barrel hatching.
Embodiment 6
1) parent fish selects
Select female rainbow trout (average weight 1.5 kilograms) and the male fresh water rainbow trout of 4 tail 2 maturations in age of 6 tail 3 maturations in age
(average weight 1.0 kilograms), started to adopt ovum, semen collection when parent fish oestruses.
2) dry method insemination
Using parent fish folder to be picked up by raun, dry moisture with gauze, the most parallel be placed on above porcelain basin, abdominal part is towards porcelain basin
Mouthful, use pressure abdomen method to squeeze in clean porcelain basin by female fish roe grain, every tail raun will obtain 1500-3500 a diameter of 4-not waited
The demersal egg of 7mm, picks up milter immediately, uses pressure abdomen method to be squeezed in basin on ovum grain by milter seminal fluid, and on band, rubber gloves is light
Light stirring, treats essence ovum mix homogeneously, and whole operation keeps room temperature at 13 DEG C.
3) germ cell is cleaned
After essence ovum mix homogeneously, being 14 DEG C by temperature, dissolved oxygen amount is that the cold spring water of more than 7mg/L rinses germ cell repeatedly
8 times, until water becomes clear, whole operation 4min.
4) imbibition
Standing 50min, allow the abundant imbibition of germ cell, after choosing the dead ovum of white, remaining successful germ cell of being fertilized is
For faint yellow or Chinese red, go to hatching barrel hatching.
91.8%, 91.0% being added up the rate of fertilization of embodiment 1-embodiment 6 germ cell, rate of fertilization is followed successively by:,
93.2%, 92.2%, 91.5%, 92.1%；Operate according to the method for artificial insemination in patent CN200910089546.8, be subject to
Essence rate is 80%.
Claims (6)
1. an artificial insemination method for rainbow trout, step mainly includes that parent fish selects, dry method is inseminated, washed by egg white and absorb water swollen
It is swollen, it is characterised in that: germ cell is cleaned and is cleaned with cold spring water.
The artificial insemination method of a kind of rainbow trout the most according to claim 1, it is characterised in that: the female rainbow in parent fish selection
The additional proportion of trout and female rainbow trout is 1:1-3:1.
The artificial insemination method of a kind of rainbow trout the most according to claim 1, it is characterised in that: in dry method Process of Insemination, control
Temperature processed is at 10-15 DEG C.
The artificial insemination method of a kind of rainbow trout the most according to claim 1, it is characterised in that: in dry method Process of Insemination, stir
Mixing speed is 50-60r/min.
The artificial insemination method of a kind of rainbow trout the most according to claim 1, it is characterised in that: the cold spring that germ cell is cleaned
Water, temperature is 10-12 DEG C, and dissolved oxygen amount is >=8mg/L.
The artificial insemination method of a kind of rainbow trout the most according to claim 1, it is characterised in that: during imbibition, it is subject to
Essence successfully germ cell is faint yellow or Chinese red.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610838487.XA CN106172146B (en) | 2016-09-21 | 2016-09-21 | A kind of artificial insemination method of rainbow trout |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610838487.XA CN106172146B (en) | 2016-09-21 | 2016-09-21 | A kind of artificial insemination method of rainbow trout |
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| Publication Number | Publication Date |
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| CN106172146A true CN106172146A (en) | 2016-12-07 |
| CN106172146B CN106172146B (en) | 2019-05-14 |
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| CN201610838487.XA Expired - Fee Related CN106172146B (en) | 2016-09-21 | 2016-09-21 | A kind of artificial insemination method of rainbow trout |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110612930A (en) * | 2019-10-15 | 2019-12-27 | 南宁学院 | Artificial breeding method for rainbow trout |
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| JP2007097518A (en) * | 2005-10-06 | 2007-04-19 | Shizuoka Prefecture | Method for rearing, breeding or preservation of rainbow trout and rainbow trout reared, bred or preserved by the method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110612930A (en) * | 2019-10-15 | 2019-12-27 | 南宁学院 | Artificial breeding method for rainbow trout |
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| CN106172146B (en) | 2019-05-14 |
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