CN104756920A - Huso dauricus female fish and amur sturgeon male fish hybridization fish breeding method - Google Patents
Huso dauricus female fish and amur sturgeon male fish hybridization fish breeding method Download PDFInfo
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Abstract
The invention discloses a huso dauricus female fish and amur sturgeon male fish hybridization fish breeding method and belongs to the technical field of aquaculture. The method includes the steps that firstly, parent selection is conducted, wherein a huso dauricus at age 14+ is selected as a female parent, and an amur sturgeon at age 8+ is selected as a male parent; secondly, parent fish breeding and reproductive regulation are conducted, wherein S1, the parent fishes are selected, S2, feeds of the parent fishes are selected, the parent fishes are fed and nutrient enrichment is performed, S3, water temperature and reproductive regulation is performed, and S4, breeding condition management and daily management are performed; thirdly, artificial fertilization and incubation are conducted, S1, egg stripping and semen collection are performed, S2, artificial fertilization is performed, S3, egg washing is performed, and S4, fertilized ovum incubation is performed; fourthly, larval fish and juvenile fish breeding is conducted. The hybridized sturgeon fry bred through the method are high in fertilization rate and incubation rate, and have the advantages of being excellent in growth trait, and specifically speaking, the hybridized sturgeon fry are quick in growing, wide in adaptability, high in stress resistance and the like, and therefore the method is suitable for breeding of sturgeons in different regions of China.
Description
Technical field
The invention belongs to technical field of aquaculture, relate to a kind of Huso huso raun and Amur Sturgeon milter hybrid breeding method.
Background technology
At present, China has become Yang Xun big country of the world, and annual production is about 21000 tons, accounts for 89% of world's sturgeon cultivation output.Along with the fast development of China's sturgeon cultivation industry, hybridization breed variety is chaotic, there is no the problems such as excellent Cultivars becomes increasingly conspicuous, and seriously constrains the sound development of China's sturgeon industry.At present, China's sturgeon cultivation kind mostly is wild and raises and train kind, and in production, breed variety is chaotic, and mostly be the chaotic crossbreed of some little plants, culture performance is low.Except China there is no sturgeon improved seeds, other sturgeon cultivation big country is as countries such as the former Soviet Union, Germany, the U.S. and Iran, and the master all cultivating applicable national breeding environment supports kind.Such as, the little huso sturgeon sturgeon (Europe huso sturgeon Husohuso ♀ × sterlet Acipenserruthenus ♂) of the former Soviet Union, the siberia platform of Germany, height first sturgeon and the paddlefish etc. of the U.S. all have in the breeding environment in native country that growth is fast, premunition is strong and the advantages such as early sexual maturity.Therefore, the sturgeon new varieties cultivating the native country cultivation of applicable China are badly in need of in China, optimize and improve the structure of China's sturgeon cultivation kind.
Amur sturgeon, sturgeon are Amur Sturgeon and Huso huso, are the special product in Heilungkiang and important economic fish, are only distributed in Heillongjiang River system.Wherein Amur Sturgeon is the native country sturgeon cultivation kind of China's independent development the earliest, because it has the advantages such as individual large, growth is fast and meat is good, and be subject to the welcome of raiser, account for more than 15% of China's cultured output, but it has the shortcoming not easily accepting artificial feed.Huso huso build is very large, propagate condition artificially under growth vigor obvious, but its sexual maturity is later than other sturgeons, is about 16-20, these cultivation shortcomings all make they cultivation, promote and be subject to certain restrictions.Heilungkiang hybrid sturgeon is the filial generation of Heilungkiang Huso huso and Amur Sturgeon, has the individuality of some to exist in the natural water area in Heilungkiang.After the Heilungkiang hybrid sturgeon sexual maturity to propagate artificially for 2007, with other artificial sturgeon breeding variety protection, show higher reproductive performance.And the cultivation experience of many raisers also shows, the growth performance of Heilungkiang hybrid sturgeon is better.Therefore, Huso huso raun and the hybrid new breed production of hybrid seeds of Amur Sturgeon milter and seed rearing are to promoting that the sound development of China's sturgeon industry has important Research Significance.
Summary of the invention
The object of this invention is to provide a kind of Huso huso raun and Amur Sturgeon milter hybrid breeding method, the method can be cultivated sturgeon new varieties and be realized scale seed breeding.Hybrid seedlings insemination rate, the incubation rate cultivated through the method are high.
The object of the invention is to be achieved through the following technical solutions:
A kind of Huso huso raun and Amur Sturgeon milter hybrid breeding method, comprising: one, Juvenile stage, and two, parent fish rearing and genital regulating, three, artificial insemination and hatching, four, larva and juvenile and juvenile fish cultivation, concrete steps are as follows:
One, Juvenile stage: female parent selects 14
+age Huso huso, male parent selects 8
+age Amur Sturgeon.
Two, parent fish rearing and genital regulating: by temperature control and fortification, promote that the sexual maturity of male and female parent population is synchronous, specific requirement is as follows:
1, parent population is preferred: the female fish parent fish of Huso huso that selection form, body colour are normally, individuality is large, healthy nothing hinders 14 ages more than, and total length reaches 190 ~ 220cm, body weight 60 ~ 90kg; Amur Sturgeon milter reaches 8 ages more than, total length 155 ~ 175cm, and body weight reaches 18 ~ 23kg; Preferred parent population is put in batches 4 × 25m parent fish rearing pond, male and female quantitative proportion is ♀: ♂=1: 1 ~ 1.5; Stocking density 20 ~ 25kg/m
3;
2, parent population feed and fortification cultivate: feeding of parent population: bait is sturgeon parent population expanded pellet diet, throw something and feed day 2 times, day feeding volume be 0.5 ~ 1wt.% of fish body body weight; Parent population fortification is cultivated: when primiparity sturgeon parent population and multiparity sturgeon parent population gonad development are to IV, start to carry out parent population fortification cultivation, and cultivate before incubating to parent population product, gonad development is to the V phase; Multiparity parent population, produce after incubating and namely to throw something and feed feed, throw something and feed every day 2 times, feeding volume is 1 ~ 1.5wt.% of parent population body weight, bait: in per kilogram foundation bait, add the chilled fish of 20wt.% and 3wt.% cellulose;
3, water temperature genital regulating: when natural temperature is down to 3 DEG C, maintains 3 DEG C of water temperature 50d; Then water temperature is from 3 DEG C of bottom outs, and every day slowly raises 1 DEG C, until when being elevated to 15 ± 0.5 DEG C, after this water temperature remains unchanged, and routine observation parent population gonad development situation;
4, breeding condition and daily management: continuous charge, running water technology, water is changed in the clear pond of timing; Condition of water quality: DO >=6.0mg/L, pH7.0 ~ 8.0, hardness 10.0mg/l; The residual bait of timely removing, blowdown.
Three, artificial insemination and hatching: squeeze ovum, semen collection → artificial insemination → wash ovum → incubating oosperm, wherein: artificial insemination, fertilization rate reached is to 90%; Hatching water temperature (15 ± 0.5) DEG C, hatches prelarva after fertilized egg 168h, incubation rate more than 85%.
Four, larva and juvenile and juvenile fish are cultivated: feedstuff series is water silk earthworm → water silk earthworm+manufactured feed → manufactured feed.Membrane prelarva 1 ~ 7 sky and water silk earthworm live bait minces rear cultivation 7 days, and cultivate water temperature (18 ± 0.5) DEG C, number of times of throwing something and feeding day is 8 times; 7 when throwing something and feeding afterwards at every turn, in water silk earthworm, mixing need be carried out in 5 ~ 7.5wt.% ratio interpolation manufactured feed to throw something and feed, within 3 days, stop afterwards adding manufactured feed, change live bait and mixed bait alternately the mode of throwing something and feeding carry out prelarva acclimatization culture, start to throw something and feed every day and coordinate number of times 1 ~ 2 time, live bait 6 ~ 7 times, within later every 1 ~ 2 day, reduce by a live bait and to throw something and feed number of times, corresponding increase manufactured feed throw something and feed number of times live, keep throw something and feed day sum constant; The manufactured feed acclimatization culture of prelarva can be completed through 7 ~ 10 days.The hybrid sturgeon seed fertilization rate of this method cultivation, incubation rate are high, and (insemination rate reaches more than 90%, incubation rate reaches more than 85%), and there is the excellent feature of the growth traits such as growth is fast, wide adaptability, strong stress resistance, be suitable for the cultivation of China different regions sturgeon.
Embodiment
Below technical scheme of the present invention is further described, but is not limited thereto, everyly technical solution of the present invention is modified or equivalent to replace, and do not depart from the spirit and scope of technical solution of the present invention, all should be encompassed in protection scope of the present invention.
A kind of Huso huso raun and Amur Sturgeon milter crossbreeding seedling-cultivating method, comprise the following steps:
One, Juvenile stage:
Female parent select 14 age above Huso huso raun, male parent select 8 age above Amur Sturgeon milter.
Two, parent fish rearing and genital regulating:
Comprise: 1, parent population is preferred; 2, parent population feed, feed and fortification; 3, water temperature genital regulating; 4, breeding condition and daily management, particular content is as follows:
1, parent population is preferred: the female fish parent fish of Huso huso that selection form, body colour are normally, individuality is large, healthy nothing hinders 14 ages more than, and total length reaches 190 ~ 220cm, body weight 60 ~ 90kg; Amur Sturgeon milter reaches 8 ages more than, total length 155 ~ 175cm, and body weight reaches 18 ~ 23kg; Preferred parent population is put in batches 4 × 25m parent fish rearing pond, male and female quantitative proportion is ♀: ♂=1: 1 ~ 1.5; Stocking density 20kg/m
3;
2, parent population feed, feed and fortification cultivate: the feed of parent population: bait is sturgeon parent population expanded pellet diet; Feeding of parent population: throw something and feed day 2 times, day feeding volume be 0.5 ~ 1wt.% of fish body body weight; Parent population fortification is cultivated: automatic control temperature control starts, in per kilogram foundation bait (sturgeon commercial feed powder), add the chilled fish of 20wt.% and 3wt.% cellulose; When primiparity sturgeon parent population and multiparity sturgeon parent population gonad development are to IV, start to carry out parent population fortification cultivation, cultivate before incubating to parent population product, gonad development, to the V phase, is thrown something and fed 2 times every day, and feeding volume is 1 ~ 1.5wt.% of parent population body weight.
3, water temperature genital regulating: water temperature regulate and control: when natural temperature is down to 3 DEG C, maintains 3 DEG C of water temperature 50d; Then water temperature is from 3 DEG C of bottom outs, and every day slowly raises 1 DEG C, until when being elevated to 15 ± 0.5 DEG C, after this water temperature remains unchanged, and routine observation parent population gonad development situation;
4, breeding condition and daily management: daily management: during parent fish rearing, continuous charge, running water technology, water is changed in the clear pond of timing; Condition of water quality: DO >=6.0mg/L, pH7.0 ~ 8.0, hardness 10.0mg/l; The residual bait of timely removing, blowdown.
Three, artificial insemination and hatching:
Comprise: 1, squeeze ovum, semen collection; 2, artificial insemination; 3, ovum is washed; 4, incubating oosperm, particular content is as follows:
Cultivate through 50d temperature control, control light and fortification, the female fish parent fish gonad development of Huso huso is to the IV phase to the V phase, and it is soft that outward appearance can be observed the full expansion of raun belly, and meanwhile, Amur Sturgeon milter sexual gland is also ripe;
1, squeeze ovum, semen collection: first fish for Huso huso parent population, after M-222 anesthesia, get ovum with Minimally Invasive Surgery, squeeze in ovum inflow washbowl and wait for fertilization; In addition pull Amur Sturgeon milter limit squish lip suction pipe collecting semen in advance out and instill another sky beaker; Within every 2 hours, gather once, 4 DEG C of Refrigerator stores are for subsequent use, carry out seminal fluid microscopy before artificial insemination, select the good seminal fluid of sperm viability and carry out artificial insemination;
2, artificial insemination: adopt dry method fertilization, 500ml cold water is added in the beaker filling seminal fluid 15ml, stir and activate sperm, the sperm that is activated is poured into rapidly to be contained in ovum container, quick stirring 5min, then add to Sheng ovum container the pure and fresh fresh water that 2000ml water temperature is 15 ± 0.5 DEG C, stir 1min with feather, whole fertilization process requires to complete in 10min;
3, wash ovum: after the 15min that has been fertilized, fertilized egg is poured into bolting silk and makes and specially wash in the fishing net of ovum, wash ovum 3 times with the pure and fresh fresh water of 15 ± 0.5 DEG C;
4, unsticking: washed fertilized egg is poured into and fills in 5 liter of 20% talcous unsticking cylinder in advance, unsticking 20 minutes;
5, incubating oosperm: hatching adopts vial-type incubator, 30cm × 60cm; Place fertilized egg 1.5L in each hatching bottle, hatching water temperature is 15 ± 0.5 DEG C, and pH7 ~ 8, fertilized egg is hatched through 168h, and prelarva demoulding is hatched.
Four, the cultivation of larva and juvenile and juvenile fish:
Support the initial stage temporarily, breeding density controls at 3 000 ~ 5 000 tails/m
2, grow breeding density before sinking to fry and drop to 2 000 ~ 3 000 tails/m
2; Membrane prelarva 1 ~ 7 sky and water silk earthworm live bait minces rear cultivation 7 days (round glass jar tank 2m × 0.4m), and cultivate water temperature (18 ± 0.5) DEG C, number of times of throwing something and feeding day is 8 times; 7 when throwing something and feeding afterwards at every turn, in water silk earthworm, mixing need be carried out in 5 ~ 7.5wt.% ratio interpolation manufactured feed (sturgeon particulate open-mouthed bait) to throw something and feed, within 3 days, stop afterwards adding manufactured feed, change live bait and mixed bait alternately the mode of throwing something and feeding carry out prelarva acclimatization culture, start to throw something and feed every day and coordinate number of times 1 ~ 2 time, live bait 6 ~ 7 times, within later every 1 ~ 2 day, reduce by a live bait and to throw something and feed number of times, corresponding increase manufactured feed throw something and feed number of times live, keep throw something and feed day sum constant.The manufactured feed acclimatization culture of prelarva can be completed through 7 ~ 10 days; Juvenile fish cultivates pond has the cement pit of the facility that shelters from heat or light or fiberglass pond to cultivate based on erection.Fry breeding density every square metre of water surface can put fry 500 ~ 1 000 tail of about 3cm in a suitable place to breed.
Claims (4)
1. Huso huso raun and an Amur Sturgeon milter hybrid breeding method, is characterized in that described method step is as follows:
One, Juvenile stage: female parent selects 14
+age Huso huso, male parent selects 8
+age Amur Sturgeon;
Two, parent fish rearing and genital regulating:
(1) parent population is preferred: the female fish parent fish of Huso huso that selection form, body colour are normally, individuality is large, healthy nothing hinders 14 ages more than, and total length reaches 190 ~ 220cm, body weight 60 ~ 90kg; Amur Sturgeon milter reaches 8 ages more than, total length 155 ~ 175cm, and body weight reaches 18 ~ 23kg; Preferred parent population is put in batches 4 × 25m parent fish rearing pond, male and female quantitative proportion is
stocking density 20 ~ 25kg/m
3;
(2) parent population feed and fortification cultivate: feeding of parent population: throw something and feed day 2 times, day feeding volume be 0.5 ~ 1wt.% of fish body body weight; Parent population fortification is cultivated: primiparity sturgeon parent population and multiparity sturgeon parent population, when gonad development is to IV, start to carry out parent population fortification cultivation, and cultivate before incubating to parent population product, gonad development, to the V phase, is thrown something and fed 2 times every day, and feeding volume is 1 ~ 1.5wt.% of parent population body weight;
(3) water temperature genital regulating: when natural temperature is down to 3 DEG C, maintains 3 DEG C of water temperature 50d; Then water temperature is from 3 DEG C of bottom outs, and every day slowly raises 1 DEG C, until when being elevated to 15 ± 0.5 DEG C, after this water temperature remains unchanged, and routine observation parent population gonad development situation;
(4) breeding condition and daily management: continuous charge, running water technology, water is changed in the clear pond of timing; Condition of water quality: DO >=6.0mg/L, pH7.0 ~ 8.0, hardness 10.0mg/l; The residual bait of timely removing, blowdown;
Three, artificial insemination and hatching: squeeze ovum, semen collection → artificial insemination → wash ovum → incubating oosperm;
Four, larva and juvenile and juvenile fish are cultivated: membrane prelarva 1 ~ 7 sky and water silk earthworm live bait minces rear cultivation 7 days, and cultivate water temperature (18 ± 0.5) DEG C, number of times of throwing something and feeding day is 8 times; 7 when throwing something and feeding afterwards at every turn, in water silk earthworm, mixing need be carried out in 5 ~ 7.5wt.% ratio interpolation manufactured feed to throw something and feed, within 3 days, stop afterwards adding manufactured feed, change live bait and mixed bait alternately the mode of throwing something and feeding carry out prelarva acclimatization culture, start to throw something and feed every day and coordinate number of times 1 ~ 2 time, live bait 6 ~ 7 times, within later every 1 ~ 2 day, reduce by a live bait and to throw something and feed number of times, corresponding increase manufactured feed throw something and feed number of times live, keep throw something and feed day sum constant; The manufactured feed acclimatization culture of prelarva can be completed through 7 ~ 10 days.
2. Huso huso raun according to claim 1 and Amur Sturgeon milter hybrid breeding method, is characterized in that in described step 2, and parent population bait is sturgeon parent population expanded pellet diet.
3. Huso huso raun according to claim 1 and Amur Sturgeon milter hybrid breeding method, is characterized in that in described step 2, and bait is cultivated in fortification: in per kilogram parent population bait, add the chilled fish of 20wt.% and 3wt.% cellulose.
4. Huso huso raun according to claim 1 and Amur Sturgeon milter hybrid breeding method, is characterized in that, in described step 3, the fertilization rate reached of artificial insemination is to 90%; Hatching water temperature (15 ± 0.5) DEG C, hatches prelarva after fertilized egg 168h, incubation rate more than 85%.
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Cited By (5)
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| CN105557586A (en) * | 2016-01-13 | 2016-05-11 | 杭州千岛湖鲟龙科技股份有限公司 | Hybrid breeding method for female Siberian sturgeon and male Amur sturgeon |
| CN106069899A (en) * | 2016-06-15 | 2016-11-09 | 中国水产科学研究院长江水产研究所 | Artificial regulatory realizes the method that acipenser dabryanus natural mating is laid eggs |
| CN107018935A (en) * | 2017-05-23 | 2017-08-08 | 中国水产科学研究院黑龙江水产研究所 | A kind of wild makeup of chum salmon seedling is put |
| CN107549059A (en) * | 2017-08-15 | 2018-01-09 | 广东省生物资源应用研究所 | A kind of Amur Sturgeon raun and paddlefish milter hybrid breeding method |
| CN109804951A (en) * | 2019-03-26 | 2019-05-28 | 海南晨海水产有限公司 | A kind of artificial hybridization breeding method of indigo plant body big spot grouper and analysis of Cromileptes altivelis |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105557586A (en) * | 2016-01-13 | 2016-05-11 | 杭州千岛湖鲟龙科技股份有限公司 | Hybrid breeding method for female Siberian sturgeon and male Amur sturgeon |
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| CN107018935B (en) * | 2017-05-23 | 2023-07-21 | 中国水产科学研究院黑龙江水产研究所 | Wild device of marijuana fry |
| CN107549059A (en) * | 2017-08-15 | 2018-01-09 | 广东省生物资源应用研究所 | A kind of Amur Sturgeon raun and paddlefish milter hybrid breeding method |
| CN107549059B (en) * | 2017-08-15 | 2020-01-07 | 广东省生物资源应用研究所 | Hybrid breeding method for female sturgeon and male paddlefish |
| CN109804951A (en) * | 2019-03-26 | 2019-05-28 | 海南晨海水产有限公司 | A kind of artificial hybridization breeding method of indigo plant body big spot grouper and analysis of Cromileptes altivelis |
| CN109804951B (en) * | 2019-03-26 | 2022-02-18 | 海南晨海水产有限公司 | Artificial crossbreeding method for large-body mackerel and camel-back bass |
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