CN106134760A - A kind of hedgehog fungus bacterial compost and cultural method thereof - Google Patents

A kind of hedgehog fungus bacterial compost and cultural method thereof Download PDF

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Publication number
CN106134760A
CN106134760A CN201510203778.7A CN201510203778A CN106134760A CN 106134760 A CN106134760 A CN 106134760A CN 201510203778 A CN201510203778 A CN 201510203778A CN 106134760 A CN106134760 A CN 106134760A
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compost
temperature
culturing room
blender
bagasse
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丁海洋
姚璐晔
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Jiangsu Lv Shu Edible Mushroom Co Ltd
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Jiangsu Lv Shu Edible Mushroom Co Ltd
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Abstract

The invention discloses a kind of hedgehog fungus bacterial compost and cultural method thereof, containing following component, include by weight percentage, bagasse 30-35, soybean cake powder 28-35, thick wood flour 15-22, wheat bran 12-15, calcium hydroxide 3-5.The manufacture method of described compost is for sieve bagasse, and blender put into by the bagasse sifted out, then pours soybean cake powder and wheat bran into, stirs 10 ~ 15 minutes;In blender, add thick wood flour, continue stirring 20 minutes;After calcium hydroxide water dissolution, uniformly it is spilled in blender;Adding water in blender, water content is maintained at 64-65%;Compost machinery is packed, carries out sterilizing, sterilizing 4 hours at 0.1Mpa pressure, at a temperature of 125 DEG C, then naturally cool to 25 DEG C.Bacterial strain unsaturated fatty acid content of the present invention is at 60-67%, higher than having unsaturated fatty acid content in Hericium erinaceus (Bull. Ex Fr.) Pers. at present.

Description

A kind of hedgehog fungus bacterial compost and cultural method thereof
Technical field
The present invention relates to field of edible fungus culture, particularly to a kind of hedgehog fungus bacterial compost and cultural method thereof.
Background technology
Hericium erinaceus (Bull. Ex Fr.) Pers. [Hericium erinaceus (Bull.ex.Fr)]Taxonomy is subordinate to Eumycota, Basidiomycotina, Hericium erinaceus (Bull. Ex Fr.) Pers. section, Hericium erinaceus (Bull. Ex Fr.) Pers. belong to (Hericium).Whole sporophore shape, as the head of monkey, color yellowish-brown, as the hair of monkey, therefore is named " hedgehog hydnum ".Hericium erinaceus (Bull. Ex Fr.) Pers. enter people dietetic life long-standing, pass on from one to another as far back as the front Shang dynasty in 3000, someone pluck Hericium erinaceus (Bull. Ex Fr.) Pers. eat." border on the sea water and soil foreign body will ": " people are the best eats hedgehog hydnum thick soup, though five meat can not and it, its custom speech is said: peaceful negative thousand kekuioilplants, is reluctant negative hedgehog hydnum thick soup.Proverb among the people: " polyphagia monkey mushroom is recovered one's youthful vigour." but due to " that thing which is rare is dear " of Hericium erinaceus (Bull. Ex Fr.) Pers., this delicacy from mountain only has imperial palace, the mansion of a prince just can enjoy, the external world only knows that Hericium erinaceus (Bull. Ex Fr.) Pers. is gourmet food.Until nineteen fifty-nine, China is successfully made artificial culture to Hericium erinaceus (Bull. Ex Fr.) Pers., and Hericium erinaceus (Bull. Ex Fr.) Pers. becomes the culturing edible fungus that China is important, and occupies first of the world in 1979 annual productions.
The nutritional labeling of Hericium erinaceus (Bull. Ex Fr.) Pers. is the highest, and in dry product, every hectogram contains 26.3 grams of protein, is two times of Lentinus Edodes.It contains up to 17 kinds of aminoacid, wherein needed by human body account for 8 kinds.Every fatty 4.2 grams of hectogram hedgehog hydnum, is genuine high protein, low-fat food, the most also rich in various vitamin and inorganic salt (Zhang Lichen, 2006).The main active substances of Hericium erinaceus (Bull. Ex Fr.) Pers. is polysaccharide, terpenoid, phytosterin compound, sugar alcohols compound, lipid, alkaloids and organic acid etc..The domestic research to Hericium erinaceus (Bull. Ex Fr.) Pers. nutritive and health protection components is concentrated mainly on Hericium Erinaceus Polysaccharide, and research to unsaturated fatty acid is the most less, research to the unsaturated fatty acid of Hericium erinaceus (Bull. Ex Fr.) Pers. at present finds, the unsaturated fatty acid contained in Hericium erinaceus (Bull. Ex Fr.) Pers. is linoleic acid, ethyl palmitate and ethyl linoleic acid, its ratio accounting is respectively 87.18%, 12.67% and 0.54%, research to hedgehog hydnum also indicates that, hericium mycelium also contains unsaturated fatty acid, the component that accounting is bigger is linoleic acid, palmitic acid and oleic acid etc., relative amount is respectively 80.89%, 14.77% and 4.34%.And unsaturated fatty acid can reduce blood cholesterol and triglyceride to have had the report of research to show at present, reducing blood viscosity, improve blood microcirculation, improve the activity of brain cell, memory reinforcing and ability of thinking etc. act on.The most how to improve unsaturated fatty acid content in Hericium erinaceus (Bull. Ex Fr.) Pers., economy and the edibility of expanding Hericium erinaceus (Bull. Ex Fr.) Pers. are the blank that current Hericium erinaceus (Bull. Ex Fr.) Pers. is cultivated.
Summary of the invention
In order to overcome drawbacks described above, the invention provides a kind of Hericium erinaceus (Bull. Ex Fr.) Pers. compost, it is provided that the source of Hericium erinaceus (Bull. Ex Fr.) Pers. unsaturated fatty acid, enable bacterial strain rich in unsaturated fatty acid.
The present invention is achieved through the following technical solutions:
A kind of hedgehog fungus bacterial compost and cultural method thereof, containing following component, include by weight percentage,
Bagasse 30-35;
Soybean cake powder 28-35;
Thick wood flour 15-22;
Wheat bran 12-15;
Calcium hydroxide 3-5.
The a length of 2-3cm of described bagasse particles, the vexed fermentation of heap more than 1 year, color is brown.
Described thick wood flour is poplar or Metasequoia glyptostroboides Hu et Cheng wood, and its width is 1-2mm, a length of 2-3cm, and the vexed fermentation of heap is no less than 60 days.
The pH value of described compost is 5.0-6.0.
The manufacture method of described compost is:
A. bagasse sieves, and blender put into by the bagasse sifted out, then pours soybean cake powder and wheat bran into, stirs 10 ~ 15 minutes;
B. in blender, add thick wood flour, continue stirring 20 minutes;
C. by after calcium hydroxide water dissolution, uniformly it is spilled in blender;
D. adding water in blender, water content is maintained at 64-65%;
E. compost machinery is packed, carry out sterilizing, sterilizing 4 hours at 0.1Mpa pressure, at a temperature of 125 DEG C, then naturally cool to 25 DEG C.
A kind of hedgehog fungus bacterial cultural method:
Strain is planted rapidly in the strain bag that claim 5 obtains by aseptic inoculation method the most routinely, the culturing room that postvaccinal bacterium bag is sent to sterilize is carried out lucifuge cultivation, the humid control of culturing room is between 65-70%, culturing room's temperature is maintained at 22 1 24 DEG C, the temperature of the 1st week culturing room should control between 24-23 DEG C, the temperature of the 2nd week culturing room should control between 21-22 DEG C, the temperature of the 3rd Zhou Shi culturing room should control between 18-20 DEG C, 27-28 days, mycelia covers with pocket, and culturing room requires lucifuge, ventilation;
G. after mycelia covers with pocket, continue to cultivate 7-8 days in the culturing room that temperature is 18-22 DEG C, then the temperature of culturing room is down to 12-15 DEG C, humidity increases to 80-85%, and irradiates by scattered light, strengthens air circulation, keep 4-5 days, form mushroom flower bud, solve opened mouth, then pocket is moved to mushroom room fruiting;
H. after mycelia covers with pocket, condition change process should be carried out, strengthen the temperature difference, giving scattered light to irradiate, and carry out stand fruiting, the strict mushroom room temperature that controls is 14 DEG C-19 DEG C, humidity is about 90%, keep mushroom house flash illumination bright by scattered light, ventilation 2-3 time of sooner or later windowing every day, ventilate about half an hour every time;
I. hedgehog hydnum grows 7-10 days, gathers in time when Hericium erinaceus (Bull. Ex Fr.) Pers. stings about 0.5cm, is taken off by sporophore with pocket knife when gathering, handle stays 1-2 centimetre, after hedgehog hydnum of once gathering, and cleaning bacterium bag mushroom root and old mycoderma, tighten bag mouth, continue cultivation and can form second batch mushroom in about 10 days.
Beneficial effects of the present invention:
Bagasse in the present invention is the vexed fermentation of heap more than 1 year, can be that hericium erinaceus strain provides enough sugars, Semen sojae atricolor is up to 85% as a kind of raw material rich in unsaturated fatty acid, the content of its unsaturated fatty acid, the most in the present invention as Hericium erinaceus (Bull. Ex Fr.) Pers. bacterial strain the source of unsaturated fatty acid.Thick wood flour, both as the carbon source of bacterial strain, can increase again the breathability of bacterium bag.Wheat bran provides nitrogen source for bacterial strain, and calcium hydroxide may be used for regulating culturing rack acid-base value.
The content of the Hericium erinaceus (Bull. Ex Fr.) Pers. bacterial strain unsaturated fatty acid that compost of the present invention is cultivated is up to 68%, higher than the 15-20% of current Hericium erinaceus (Bull. Ex Fr.) Pers. unsaturated fatty acid content.
Detailed description of the invention
Embodiment 1
Take bagasse 30, soybean cake powder 32, thick wood flour 20, wheat bran 15, calcium hydroxide 3 by weight percentage.
The manufacture method of compost is:
A. bagasse sieves, and blender put into by the bagasse sifted out, then pours soybean cake powder and wheat bran into, stirs 10 ~ 15 minutes;
B. in blender, add thick wood flour, continue stirring 20 minutes;
C. by after calcium hydroxide water dissolution, uniformly it is spilled in blender;
D. adding water in blender, water content is maintained at 64-65%;
E. compost machinery is packed, carry out sterilizing, sterilizing 4 hours at 0.1Mpa pressure, at a temperature of 125 DEG C, then naturally cool to 25 DEG C.
In the present embodiment, a length of 2-3cm of this bagasse particles, the vexed fermentation of heap more than 1 year, color is brown.
This thick wood flour is poplar or Metasequoia glyptostroboides Hu et Cheng wood, and its width is 1-2mm, a length of 2-3cm, and the vexed fermentation of heap is no less than 60 days.
The pH value of compost is 5.0-6.0.
A kind of hedgehog fungus bacterial cultural method, comprises the steps:
Strain is planted rapidly in the strain bag that claim 5 obtains by aseptic inoculation method the most routinely, the culturing room that postvaccinal bacterium bag is sent to sterilize is carried out lucifuge cultivation, the humid control of culturing room is between 65-70%, culturing room's temperature is maintained at 22 1 24 DEG C, the temperature of the 1st week culturing room should control between 24-23 DEG C, the temperature of the 2nd week culturing room should control between 21-22 DEG C, the temperature of the 3rd Zhou Shi culturing room should control between 18-20 DEG C, 27-28 days, mycelia covers with pocket, and culturing room requires lucifuge, ventilation;
G. after mycelia covers with pocket, continue to cultivate 7-8 days in the culturing room that temperature is 18-22 DEG C, then the temperature of culturing room is down to 12-15 DEG C, humidity increases to 80-85%, and irradiates by scattered light, strengthens air circulation, keep 4-5 days, form mushroom flower bud, solve opened mouth, then pocket is moved to mushroom room fruiting;
H. after mycelia covers with pocket, condition change process should be carried out, strengthen the temperature difference, giving scattered light to irradiate, and carry out stand fruiting, the strict mushroom room temperature that controls is 14 DEG C-19 DEG C, humidity is about 90%, keep mushroom house flash illumination bright by scattered light, ventilation 2-3 time of sooner or later windowing every day, ventilate about half an hour every time;
I. hedgehog hydnum grows 7-10 days, gathers in time when Hericium erinaceus (Bull. Ex Fr.) Pers. stings about 0.5cm, is taken off by sporophore with pocket knife when gathering, handle stays 1-2 centimetre, after hedgehog hydnum of once gathering, and cleaning bacterium bag mushroom root and old mycoderma, tighten bag mouth, continue cultivation and can form second batch mushroom in about 10 days.
Embodiment 2
Take bagasse 32, soybean cake powder 35, thick wood flour 15, wheat bran 13, calcium hydroxide 5 by weight percentage.
The manufacture method of compost is:
A. bagasse sieves, and blender put into by the bagasse sifted out, then pours soybean cake powder and wheat bran into, stirs 10 ~ 15 minutes;
B. in blender, add thick wood flour, continue stirring 20 minutes;
C. by after calcium hydroxide water dissolution, uniformly it is spilled in blender;
D. adding water in blender, water content is maintained at 64-65%;
E. compost machinery is packed, carry out sterilizing, sterilizing 4 hours at 0.1Mpa pressure, at a temperature of 125 DEG C, then naturally cool to 25 DEG C.
In the present embodiment, a length of 2-3cm of this bagasse particles, the vexed fermentation of heap more than 1 year, color is brown.
This thick wood flour is poplar or Metasequoia glyptostroboides Hu et Cheng wood, and its width is 1-2mm, a length of 2-3cm, and the vexed fermentation of heap is no less than 60 days.
The pH value of compost is 5.0-6.0.
A kind of hedgehog fungus bacterial cultural method, comprises the steps:
Strain is planted rapidly in the strain bag that claim 5 obtains by aseptic inoculation method the most routinely, the culturing room that postvaccinal bacterium bag is sent to sterilize is carried out lucifuge cultivation, the humid control of culturing room is between 65-70%, culturing room's temperature is maintained at 22 1 24 DEG C, the temperature of the 1st week culturing room should control between 24-23 DEG C, the temperature of the 2nd week culturing room should control between 21-22 DEG C, the temperature of the 3rd Zhou Shi culturing room should control between 18-20 DEG C, 27-28 days, mycelia covers with pocket, and culturing room requires lucifuge, ventilation;
G. after mycelia covers with pocket, continue to cultivate 7-8 days in the culturing room that temperature is 18-22 DEG C, then the temperature of culturing room is down to 12-15 DEG C, humidity increases to 80-85%, and irradiates by scattered light, strengthens air circulation, keep 4-5 days, form mushroom flower bud, solve opened mouth, then pocket is moved to mushroom room fruiting;
H. after mycelia covers with pocket, condition change process should be carried out, strengthen the temperature difference, giving scattered light to irradiate, and carry out stand fruiting, the strict mushroom room temperature that controls is 14 DEG C-19 DEG C, humidity is about 90%, keep mushroom house flash illumination bright by scattered light, ventilation 2-3 time of sooner or later windowing every day, ventilate about half an hour every time;
I. hedgehog hydnum grows 7-10 days, gathers in time when Hericium erinaceus (Bull. Ex Fr.) Pers. stings about 0.5cm, is taken off by sporophore with pocket knife when gathering, handle stays 1-2 centimetre, after hedgehog hydnum of once gathering, and cleaning bacterium bag mushroom root and old mycoderma, tighten bag mouth, continue cultivation and can form second batch mushroom in about 10 days.
Embodiment 3
Take bagasse 35, soybean cake powder 28, thick wood flour 21, wheat bran 12, calcium hydroxide 4 by weight percentage.
The manufacture method of compost is:
A. bagasse sieves, and blender put into by the bagasse sifted out, then pours soybean cake powder and wheat bran into, stirs 10 ~ 15 minutes;
B. in blender, add thick wood flour, continue stirring 20 minutes;
C. by after calcium hydroxide water dissolution, uniformly it is spilled in blender;
D. adding water in blender, water content is maintained at 64-65%;
E. compost machinery is packed, carry out sterilizing, sterilizing 4 hours at 0.1Mpa pressure, at a temperature of 125 DEG C, then naturally cool to 25 DEG C.
In the present embodiment, a length of 2-3cm of this bagasse particles, the vexed fermentation of heap more than 1 year, color is brown.
This thick wood flour is poplar or Metasequoia glyptostroboides Hu et Cheng wood, and its width is 1-2mm, a length of 2-3cm, and the vexed fermentation of heap is no less than 60 days.
The pH value of compost is 5.0-6.0.
A kind of hedgehog fungus bacterial cultural method, comprises the steps:
Strain is planted rapidly in the strain bag that claim 5 obtains by aseptic inoculation method the most routinely, the culturing room that postvaccinal bacterium bag is sent to sterilize is carried out lucifuge cultivation, the humid control of culturing room is between 65-70%, culturing room's temperature is maintained at 22 1 24 DEG C, the temperature of the 1st week culturing room should control between 24-23 DEG C, the temperature of the 2nd week culturing room should control between 21-22 DEG C, the temperature of the 3rd Zhou Shi culturing room should control between 18-20 DEG C, 27-28 days, mycelia covers with pocket, and culturing room requires lucifuge, ventilation;
G. after mycelia covers with pocket, continue to cultivate 7-8 days in the culturing room that temperature is 18-22 DEG C, then the temperature of culturing room is down to 12-15 DEG C, humidity increases to 80-85%, and irradiates by scattered light, strengthens air circulation, keep 4-5 days, form mushroom flower bud, solve opened mouth, then pocket is moved to mushroom room fruiting;
H. after mycelia covers with pocket, condition change process should be carried out, strengthen the temperature difference, giving scattered light to irradiate, and carry out stand fruiting, the strict mushroom room temperature that controls is 14 DEG C-19 DEG C, humidity is about 90%, keep mushroom house flash illumination bright by scattered light, ventilation 2-3 time of sooner or later windowing every day, ventilate about half an hour every time;
I. hedgehog hydnum grows 7-10 days, gathers in time when Hericium erinaceus (Bull. Ex Fr.) Pers. stings about 0.5cm, is taken off by sporophore with pocket knife when gathering, handle stays 1-2 centimetre, after hedgehog hydnum of once gathering, and cleaning bacterium bag mushroom root and old mycoderma, tighten bag mouth, continue cultivation and can form second batch mushroom in about 10 days.
The unsaturated fatty acid content determination data of three embodiment Hericium erinaceus (Bull. Ex Fr.) Pers. bacterial strains:
Embodiment 1 Embodiment 2 Embodiment 3
61.06% 67.82% 67.78%

Claims (6)

1. a hedgehog fungus bacterial compost and cultural method thereof, it is characterised in that: containing following component, include by weight percentage,
Bagasse 30-35;
Soybean cake powder 28-35;
Thick wood flour 15-22;
Wheat bran 12-15;
Calcium hydroxide 3-5.
A kind of hedgehog fungus bacterial compost the most according to claim 1 and cultural method thereof, it is characterised in that: a length of 2-3cm of described bagasse particles, the vexed fermentation of heap more than 1 year, color is brown.
A kind of hedgehog fungus bacterial compost the most according to claim 1 and cultural method thereof, it is characterised in that: described thick wood flour is poplar or Metasequoia glyptostroboides Hu et Cheng wood, and its width is 1-2mm, a length of 2-3cm, and the vexed fermentation of heap is no less than 60 days.
A kind of hedgehog fungus bacterial compost the most according to claim 1 and cultural method thereof, it is characterised in that: the pH value of described compost is 5.0-6.0.
A kind of hedgehog fungus bacterial compost the most according to claim 1 and cultural method thereof, it is characterised in that: the manufacture method of described compost is,
A. bagasse sieves, and blender put into by the bagasse sifted out, then pours soybean cake powder and wheat bran into, stirs 10 ~ 15 minutes;
B. in blender, add thick wood flour, continue stirring 20 minutes;
C. by after calcium hydroxide water dissolution, uniformly it is spilled in blender;
D. adding water in blender, water content is maintained at 64-65%;
E. compost machinery is packed, carry out sterilizing, sterilizing 4 hours at 0.1Mpa pressure, at a temperature of 125 DEG C, then naturally cool to 25 DEG C.
6. a hedgehog fungus bacterial cultural method, it is characterised in that:
Strain is planted rapidly in the strain bag that claim 5 obtains by aseptic inoculation method the most routinely, the culturing room that postvaccinal bacterium bag is sent to sterilize is carried out lucifuge cultivation, the humid control of culturing room is between 65-70%, culturing room's temperature is maintained at 22 1 24 DEG C, the temperature of the 1st week culturing room should control between 24-23 DEG C, the temperature of the 2nd week culturing room should control between 21-22 DEG C, the temperature of the 3rd Zhou Shi culturing room should control between 18-20 DEG C, 27-28 days, mycelia covers with pocket, and culturing room requires lucifuge, ventilation;
G. after mycelia covers with pocket, continue to cultivate 7-8 days in the culturing room that temperature is 18-22 DEG C, then the temperature of culturing room is down to 12-15 DEG C, humidity increases to 80-85%, and irradiates by scattered light, strengthens air circulation, keep 4-5 days, form mushroom flower bud, solve opened mouth, then pocket is moved to mushroom room fruiting;
H. after mycelia covers with pocket, condition change process should be carried out, strengthen the temperature difference, giving scattered light to irradiate, and carry out stand fruiting, the strict mushroom room temperature that controls is 14 DEG C-19 DEG C, humidity is about 90%, keep mushroom house flash illumination bright by scattered light, ventilation 2-3 time of sooner or later windowing every day, ventilate about half an hour every time;
I. hedgehog hydnum grows 7-10 days, gathers in time when Hericium erinaceus (Bull. Ex Fr.) Pers. stings about 0.5cm, is taken off by sporophore with pocket knife when gathering, handle stays 1-2 centimetre, after hedgehog hydnum of once gathering, and cleaning bacterium bag mushroom root and old mycoderma, tighten bag mouth, continue cultivation and can form second batch mushroom in about 10 days.
CN201510203778.7A 2015-04-27 2015-04-27 A kind of hedgehog fungus bacterial compost and cultural method thereof Pending CN106134760A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107371801A (en) * 2017-08-14 2017-11-24 佛山推启农业研究院(普通合伙) A kind of artificial cultivation method for improving hickory chick unsaturated fatty acid content
CN109496687A (en) * 2019-01-25 2019-03-22 长沙地晟农业科技有限公司 A kind of hedgehog hydnum eats implantation methods

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107371801A (en) * 2017-08-14 2017-11-24 佛山推启农业研究院(普通合伙) A kind of artificial cultivation method for improving hickory chick unsaturated fatty acid content
CN109496687A (en) * 2019-01-25 2019-03-22 长沙地晟农业科技有限公司 A kind of hedgehog hydnum eats implantation methods

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