CN102301914B - Technology for culturing Pleurotus eryngii - Google Patents
Technology for culturing Pleurotus eryngii Download PDFInfo
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- CN102301914B CN102301914B CN 201110198641 CN201110198641A CN102301914B CN 102301914 B CN102301914 B CN 102301914B CN 201110198641 CN201110198641 CN 201110198641 CN 201110198641 A CN201110198641 A CN 201110198641A CN 102301914 B CN102301914 B CN 102301914B
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Abstract
The invention relates to the culture field of edible fungus, and discloses a technology for culturing Pleurotus eryngii. In the technology for culturing Pleurotus eryngii, ultraviolet light of which the intensity is 1.5-2.5mu w/cm<2> is used for irradiating Pleurotus eryngii fungus inoculated on a culture medium for 5-15min every 2.5-3.5h until the culture medium is completely covered by the Pleurotus eryngi hypha, and then, inorganic zinc nutrient solution is added into the culture medium until the Pleurotus eryngii is mature. The culturing technology adopts the ultraviolet induction technology to cause the Pleurotus eryngii to fully convert inorganic zinc into organic zinc, thereby obviously improving organic zinc content, and improving the nutrient quality of the Pleurotus eryngii.
Description
Technical field
The present invention relates to field of edible fungus culture, be specifically related to a kind of Xingbao mushroom and culture technique thereof.
Background technology
Xingbao mushroom is called perverse celery and is picked up the ears, and is under the jurisdiction of the Basidiomycetes in the Eumycota, is that the rare edible mushroom new varieties edible, medicinal, dietotherapy that integrate are successfully cultivated in the China recent years exploitation.Xingbao mushroom has almond flavor, not only meat plumpness, fresh and tender, the taste delicate fragrance of mouthfeel, and also nutritious.Studies show that Xingbao mushroom is rich in mineral matters such as protein, carbohydrate, vitamin and calcium, magnesium, copper.In addition, Xingbao mushroom also have reducing blood lipid, norcholesterol, promotion gastro-intestinal digestion, strengthen body immunity, anticancer, prevent effects such as cardiovascular disease and beauty treatment.
In order further to improve the quality of Xingbao mushroom; enrich its nutrient component; in the cultivation process of present stage Xingbao mushroom, can add the nutrient solution that some contain trace element usually, utilize the biological transformation of Xingbao mushroom then, the trace element in the nutrient solution is converted into self own.Because zinc is one of teleorganic trace element, it is the catalysis factor of the many activated acids of human body, participate in planting surplus in the of 300 in the body composition of acid and functional protein, has biochemical functions widely, so when Xinbao mushroom culturing, generally add the inorganic zinc nutrient solution at present, utilize the metabolism of Xingbao mushroom to transform then, inorganic zinc is attached on self the macromolecular substances (as polysaccharide and protein) and forms organic zinc and carry out enrichment.But present culture technique can't impel Xingbao mushroom to absorb conversion zinc element efficiently, makes that the content of the interior organic zinc of its body is lower, can't further improve the nutritional quality of Xingbao mushroom.
Summary of the invention
In view of this, the object of the present invention is to provide a kind of Xingbao mushroom and culture technique thereof, make this culture technique can improve organic zinc content in the Xingbao mushroom.
For achieving the above object, the invention provides following technical scheme:
A kind of culture technique of Xingbao mushroom is with 1.5-2.5 μ w/cm
2The ultraviolet light of intensity, the Xingbao mushroom bacterium 5-15min that is inoculated on the medium every 2.5-3.5h irradiation is covered by the Xingbao mushroom mycelia entirely to medium, adds the inorganic zinc nutrient solution to the Xingbao mushroom maturation then in medium.
Wherein, judge whether medium is covered by the Xingbao mushroom mycelia entirely and whether Xingbao mushroom is ripe, is known to those skilled in the art.The method of inoculation Xingbao mushroom bacterium is this area routine techniques means, and the Xingbao mushroom bacterial classification can be by commercially available acquisition.
Xingbao mushroom can form some novel proteins or make that some albumen is synthetic to be strengthened when being subjected to containing the coercing of zinc element mineral salt, these albumen are called the salt stress protein, and they can form zinc albuminate in conjunction with zinc ion.But existing culture technique can't make the salt stress protein efficiently in conjunction with zinc ion, makes that organic zinc (the being zinc albuminate) content in the Xingbao mushroom is lower.
Therefore, the present invention is shone Xingbao mushroom with ultraviolet ray earlier, and then adds the inorganic zinc nutrient solution, is preferably by mass volume ratio 1kg:4mL, and add the inorganic zinc nutrient solution one time every day in medium.Wherein, described inorganic zinc nutrient solution comprises that mass fraction is that 0.4-0.5% zinc sulphate and mass fraction are the 7-9% medical stone, and surplus is water, is preferably to comprise that mass fraction is that 0.45% zinc sulphate and mass fraction are 8.6% medical stone, and surplus is water.
Ultraviolet ray can make Xingbao mushroom strengthen synthetic salt adverse circumstance in conjunction with the zinc ion ability, and then adds the inorganic zinc nutrient solution, impels zinc albuminate synthetic in a large number, makes Xingbao mushroom effectively absorb the zinc element, and is converted into organic zinc and carries out enrichment.Can damage mycelia because uitraviolet intensity is too high, cross the low poor effect that zinc ion is absorbed of then inducing, the strength control of described ultraviolet light of the present invention is at 1.5-2.5 μ w/cm
2, be preferably 2 μ w/cm
2In the specific implementation, the present invention can adopt the uviol lamp of 40w, is suspended on 20 bag culture mediums, and distance is 40cm.Wherein, the irradiation frequency of described ultraviolet light is preferably every 3h irradiation 10min.
Medium of the present invention is that 39.4% water, mass fraction are that 36.4% cotton seed hulls, corncob, straw or wood chip, mass fraction are that 12.1% wheat bran, corn flour or rice bran and mass fraction are that 12.1% cottonseed oil meal or sunflower oil meal are formed by mass fraction preferably, is that 39.4% water, mass fraction are that 36.4% cotton seed hulls, mass fraction are that 12.1% wheat bran and mass fraction are that 12.1% cottonseed oil meal is formed by mass fraction more preferably.
The present invention also provides a kind of Xingbao mushroom of being planted out by culture technique of the present invention.Xingbao mushroom of the present invention is through the organic zinc content detection, be the conventional cultivation technology Xingbao mushroom of planting out organic zinc content 36-40 doubly, show that culture technique of the present invention can significantly improve Xingbao mushroom organic zinc content.
By above technical scheme as can be known, culture technique of the present invention is by adopting the uv induction technology, and making Xingbao mushroom fully transform inorganic zinc is organic zinc, has significantly improved its organic zinc content, has improved the nutritional quality of Xingbao mushroom.
Embodiment:
The invention discloses a kind of Xingbao mushroom and culture technique thereof, those skilled in the art can use for reference this paper content, suitably improve technological parameter and realize.Special needs to be pointed out is that all similarly replace and change apparent to those skilled in the art, they all are regarded as being included in the present invention.Culture technique of the present invention is described by preferred embodiment, the related personnel obviously can change or suitably change and combination methods and applications as herein described in not breaking away from content of the present invention, spirit and scope, realizes and use the technology of the present invention.
Be described further with regard to a kind of Xingbao mushroom provided by the invention and culture technique thereof below.
Embodiment 1: culture technique of the present invention
Take by weighing zinc sulphate 5kg, medical stone 95kg, water 1000kg, adopt the homogenizer high speed shear, it is standby to be prepared into the inorganic zinc nutrient solution;
Take by weighing cotton seed hull 60kg, wheat bran 20kg, cottonseed oil meal 20kg, and add water and mix the preparation medium, make moisture content in medium control simultaneously about 65%, adopt packed year medium of high density polyethylene (HDPE) bacterium of 23 * 46 * 0.005cm then, every sacked material amount is 3kg;
With the high-temperature sterilization respectively of the medium after inorganic zinc nutrient solution and the pack, 100 ℃ of sterilising temps, sterilization time are pressurize 10 hours behind the last gas; After treating that bag temperature drop to 28 ℃ is down, inoculate operation, with the medium splitting, punch, inoculation, inoculation method carries out routinely;
Cultivate, relative moisture is controlled about 70%, and temperature is 22-24 ℃; Unlatching is installed in the ultraviolet lamp of cultivating in the canopy, and ultraviolet lamp 40w, every lamp be according to 20 bag culture mediums, apart from lamp apart from 40cm, every 3 hours photographs 10 minutes; After treating that mycelia is covered with bag, inject the inorganic zinc nutrient solution, adopt the drip irrigation mode, apart from the edges at two ends 1/3 bacterium bag length place of bacterium bag, insert 2 tubules perpendicular to the bacterium bag respectively, drip irrigation is injected in the medium, inject every day once and gather in the crops until the Xingbao mushroom maturation, each every bag splashes into inorganic zinc nutrient solution 12mL.
Embodiment 2: culture technique of the present invention
Take by weighing zinc sulphate 4.4kg, medical stone 77kg, water 1018.6kg, adopt the homogenizer high speed shear, it is standby to be prepared into the inorganic zinc nutrient solution;
Take by weighing corncob 60kg, corn flour 20kg, sunflower oil meal 20kg, and add water and mix the preparation medium, make moisture content in medium control simultaneously about 65%, adopt packed year medium of high density polyethylene (HDPE) bacterium of 23 * 46 * 0.005cm then, every sacked material amount is 3kg;
With the high-temperature sterilization respectively of the medium after inorganic zinc nutrient solution and the pack, 100 ℃ of sterilising temps, sterilization time are pressurize 8 hours behind the last gas; After treating that bag temperature drop to 28 ℃ is down, inoculate operation, with the medium splitting, punch, inoculation, inoculation method carries out routinely;
Cultivate, relative moisture is controlled about 70%, and temperature is 22-24 ℃; Unlatching is installed in the ultraviolet lamp of cultivating in the canopy, and ultraviolet lamp 30w, every lamp be according to 20 bag culture mediums, apart from lamp apart from 40cm, every 3.5 hours photographs 15 minutes; After treating that mycelia is covered with bag, inject the inorganic zinc nutrient solution, adopt the drip irrigation mode, apart from the edges at two ends 1/3 bacterium bag length place of bacterium bag, insert 2 tubules perpendicular to the bacterium bag respectively, drip irrigation is injected in the medium, inject every day once and gather in the crops until the Xingbao mushroom maturation, each every bag splashes into inorganic zinc nutrient solution 12mL.
Embodiment 3: culture technique of the present invention
Take by weighing zinc sulphate 5.5kg, medical stone 99kg, water 995.5kg, adopt the homogenizer high speed shear, it is standby to be prepared into the inorganic zinc nutrient solution;
Take by weighing straw 60kg, rice bran 20kg, cottonseed oil meal 20kg, and add water and mix the preparation medium, make moisture content in medium control simultaneously about 65%, adopt packed year medium of high density polyethylene (HDPE) bacterium of 23 * 46 * 0.005cm then, every sacked material amount is 3kg;
With the high-temperature sterilization respectively of the medium after inorganic zinc nutrient solution and the pack, 100 ℃ of sterilising temps, sterilization time are pressurize 12 hours behind the last gas; After treating that bag temperature drop to 28 ℃ is down, inoculate operation, with the medium splitting, punch, inoculation, inoculation method carries out routinely;
Cultivate, relative moisture is controlled about 70%, and temperature is 22-24 ℃; Unlatching is installed in the ultraviolet lamp of cultivating in the canopy, and ultraviolet lamp 50w, every lamp be according to 20 bag culture mediums, apart from lamp apart from 40cm, every 2.5 hours photographs 5 minutes; After treating that mycelia is covered with bag, inject the inorganic zinc nutrient solution, adopt the drip irrigation mode, apart from the edges at two ends 1/3 bacterium bag length place of bacterium bag, insert 2 tubules perpendicular to the bacterium bag respectively, drip irrigation is injected in the medium, inject every day once and gather in the crops until the Xingbao mushroom maturation, each every bag splashes into inorganic zinc nutrient solution 12mL.
Embodiment 4: the comparative trial of culture technique of the present invention
Adopt existing planting almond abalone mushroom technology plantation (namely without uv induction), Xingbao mushroom organic zinc content (unit: ppm is detected in ripe back, namely account for the concentration that the part per million of complete soln quality is represented with the solute quality, also claim ppm, the quality of contained solute in the popular solution that is interpreted as 1,000,000 parts of weight), and with the Xingbao mushroom contrast of adopting embodiment 1-3 culture technique to plant out, the results are shown in Table 1.
The comparative analysis of table 1 organic zinc content
| ? | Embodiment 1 | Embodiment 2 | Embodiment 3 | Existing culture technique |
| Organic zinc content | 175ppm | 160ppm | 167ppm | 4.41ppm |
By table 1 result as can be known, culture technique of the present invention can significantly improve Xingbao mushroom organic zinc content, be the 36-40 of the existing culture technique Xingbao mushroom of planting out doubly.
The above only is preferred embodiment of the present invention; should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the principle of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (7)
1. the culture technique of an Xingbao mushroom is characterized in that, with 1.5-2.5 μ w/cm
2The ultraviolet light of intensity, the Xingbao mushroom bacterium 5-15min that is inoculated on the medium every 2.5-3.5h irradiation is covered by the Xingbao mushroom mycelia entirely to medium, adds the inorganic zinc nutrient solution to the Xingbao mushroom maturation then in medium; Wherein, the distance of described ultraviolet light and medium is 40cm, and described inorganic zinc nutrient solution comprises that mass fraction is that 0.4-0.5% zinc sulphate and mass fraction are the 7-9% medical stone, and surplus is water.
2. according to the described culture technique of claim 1, it is characterized in that described ultraviolet light intensity is 2 μ w/cm
2
3. according to the described culture technique of claim 1, it is characterized in that the irradiation frequency of described ultraviolet light is every 3h irradiation 10min.
4. according to the described culture technique of claim 1, it is characterized in that described medium is that 65% water, mass fraction are that 21% cotton seed hulls, corncob or straw, mass fraction are that 7% wheat bran, corn flour or rice bran and mass fraction are that 7% cottonseed oil meal or sunflower oil meal are formed by mass fraction.
5. according to the described culture technique of claim 1, it is characterized in that the described inorganic zinc nutrient solution that adds in the medium is specially:
Press mass volume ratio 1kg:4mL, add the inorganic zinc nutrient solution one time every day in medium.
6. according to the described culture technique of claim 1, it is characterized in that described inorganic zinc nutrient solution comprises that mass fraction is that 0.45% zinc sulphate and mass fraction are 8.6% medical stone, surplus is water.
7. any Xingbao mushroom that described culture technique is planted out of claim 1-5.
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103387446B (en) * | 2013-07-08 | 2015-11-25 | 安徽金麒麟农业科技有限公司 | A kind of planting almond abalone mushroom base and cultivating method thereof |
| CN103626553B (en) * | 2013-12-03 | 2015-03-25 | 邬金梅 | Method utilizing sunflower by-products to manufacture white fungus cultivation material |
| CN104756751B (en) * | 2014-01-07 | 2020-01-07 | 陕西省微生物研究所 | Edible fungus bag cultivation method for simulating natural rainfall |
| CN104303832A (en) * | 2014-09-28 | 2015-01-28 | 铜陵市香江食用菌种植有限责任公司 | Method for cultivating coprinus comatus |
| CN104737775A (en) * | 2015-03-07 | 2015-07-01 | 彭阳县福泰菌业有限责任公司 | Organic pleurotus eryngii production process |
| CN109601860A (en) * | 2019-01-11 | 2019-04-12 | 中南林业科技大学 | A method of sprouted unpolished rice, which is dried, using radio-frequency technique prepares full fat rice bran |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3996916B2 (en) * | 2004-07-12 | 2007-10-24 | 社団法人長野県農村工業研究所 | New eringi strain |
| CN101182266A (en) * | 2007-10-31 | 2008-05-21 | 杭州农发科技开发有限公司 | Method for planting selenium-enriched pleurotus eryngii and culture medium thereof |
| CN101755615A (en) * | 2009-12-31 | 2010-06-30 | 张寿禄 | Cultivation method of high trace element edible and medical fungi |
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| JPS5747423A (en) * | 1980-09-03 | 1982-03-18 | Kyowa Hakko Kogyo Kk | Cultivation of jew's-ear |
| JPH07184473A (en) * | 1993-12-28 | 1995-07-25 | Masakichi Matsui | Method for artificially cultivating mushroom of genus pleurotus |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3996916B2 (en) * | 2004-07-12 | 2007-10-24 | 社団法人長野県農村工業研究所 | New eringi strain |
| CN101182266A (en) * | 2007-10-31 | 2008-05-21 | 杭州农发科技开发有限公司 | Method for planting selenium-enriched pleurotus eryngii and culture medium thereof |
| CN101755615A (en) * | 2009-12-31 | 2010-06-30 | 张寿禄 | Cultivation method of high trace element edible and medical fungi |
Non-Patent Citations (1)
| Title |
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| 王振河等.杏鲍菇菌丝营养生理特性研究.《河南师范大学学报（自然科学版）》.2007,第35卷(第4期), * |
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