CN104311329A - Culture medium capable of increasing nutrient content of agrocybe cylindracea - Google Patents
Culture medium capable of increasing nutrient content of agrocybe cylindracea Download PDFInfo
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- CN104311329A CN104311329A CN201410556228.9A CN201410556228A CN104311329A CN 104311329 A CN104311329 A CN 104311329A CN 201410556228 A CN201410556228 A CN 201410556228A CN 104311329 A CN104311329 A CN 104311329A
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- substratum
- tea tree
- wood chip
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention relates to a culture medium capable increasing nutrient content of agrocybe cylindracea. The culture medium is prepared by adopting the following ingredients in parts by weight: 15 to 20 parts of peach wood chips, 20 to 25 parts of willow wood chips, 10 to 15 parts of camellia seed shell, 1 to 3 parts of soybean powder, 0.5 to 1.5 parts of monopotassium phosphate, 3 to 6 parts of camellia oil bran, 0.5 to 1 part of magnesium sulfate, 2 to 4 pats of agar strip, 1 to 3 parts of gypsum, 0.5 to 2 parts of defoaming agent, and the balance of pH adjusting agent and water. A process of culturing by use of the culture medium comprises the following specific steps: firstly adding a fermentation bed germ solution into the culture medium to be fermented, and turning the culture medium; charging the well-fermented culture medium into a single-opening plastic bag, inoculating and culturing the agrocybe cylindracea, and managing the fruiting. The aged peach and winnow wood chips are selected as the culture medium, polysaccharide and monosaccharide decomposed by the peach and winnow wood chips can be absorbed by the agrocybe cylindracea, the camellia oil bran and other nutritional ingredients are collectively used as the culture medium of the agrocybe cylindracea, so that a good ecological environment can be provided for the growth and propagation, and the yield and nutrient content of the agrocybe cylindracea can be obviously increased.
Description
Technical field
The present invention relates to a kind of fungi culture medium, be specifically related to a kind of substratum improving tea tree mushroom nutritive ingredient.
Background technology
Tea tree mushroom belongs to Basidiomycotina, Fen Xiu umbrella section, is a kind of edible mushrooms of development in recent years.Tea tree mushroom contains the multiple amino acids of needed by human body, as L-glutamic acid, aspartic acid and glycine, especially human body self 8 seed amino acids that can not synthesize, in addition, tea tree mushroom is also containing multi mineral prime elements such as the dextran of needed by human body, mycoprotein, vitamin B group, iron, potassium, zinc and selenium.Tea tree mushroom total reducing sugar reaches 54.36%, raw sugar reaches 29.96%, and crude protein reaches 27.51%, and protein content is up to 19.55%, and these effective constituents above-mentioned obviously exceed other mushrooms.Tea tree mushroom delicious flavour, gives off a strong fragrance, fresh and crisp, no matter cooks soup or cooking, is all a good selection.And theory of traditional Chinese medical science is thought, tea tree mushroom is put down, Gan Wen, nontoxic, has the effects such as kidney tonifying, diuresis, invigorating the spleen, antidiarrheal, to suffering from a deficiency of the kidney, frequent micturition, oedema and rheumatism and obese patient etc. all have desirable effect, also has auxiliary treatment function to children's's low-heat, bed-wetting etc.Because tea tree mushroom is of high nutritive value, mouthfeel is good, and therefore, tea tree mushroom has become the comparatively ideal protective foods of people at present, is one of delicious rare edible mushrooms.
Because tea tree mushroom has desirable edible and pharmaceutical use, people also increase day by day to its demand, and therefore, large quantities of artificial growth tea tree mushroom seems very necessary.At present, be main for the substratum of planting tea tree mushroom mainly with corn cob and cotton seed hulls etc., before cultivation, often need that substratum is carried out high temperature carry out bacterium 12-18h, and substratum material is often based on straw, nutritive ingredient is low, and often there are pesticide residue, therefore, the tea tree mushroom quality of cultivation is all poor.For this reason, the invention provides one and can improve tea tree mushroom output, improve the substratum of its nutritive ingredient.
Summary of the invention
For above-mentioned the deficiencies in the prior art, the invention provides a kind of substratum improving tea tree mushroom output and rich in nutrition content.
Technical scheme of the present invention is as follows:
Improve a substratum for tea tree mushroom nutritive ingredient, described substratum is made up of the component comprising following weight part:
Peach wood chip 15-20,
Willow wood chip 20-25,
Camellia shell 10-15,
Analysis for soybean powder 1-3,
Potassium primary phosphate 0.5-1.5,
Camellia oil bran 3-6,
Magnesium sulfate 0.5-1,
Agar strip 2-4,
Gypsum 1-3,
Defoamer 0.5-2,
PH conditioning agent,
Water surplus.
Preferably, described peach wood chip and willow wood chip are through the aging wood chip of 30-40d Exposure to Sunlight.
Preferably, described peach wood chip and the weight ratio of willow wood chip are 1:1.5.
Preferably, described peach wood chip and the length of willow wood chip are 0.5-0.9cm.
Preferably, described camellia shell is pulverizing rear surface diameter is the solid particulate of 0.3-0.8cm.
Preferably, described water is the distilled water after high-temperature boiling 15-30min.
Preferably, the water content of described agar strip is 5-8%, and the water content of described substratum is 60-65%.
Preferably, described PH conditioning agent is selected from the one in the aqueous sodium hydroxide solution of 0.1-0.3mol/L or the aqueous hydrochloric acid of 0.02-0.05mol/L, and the PH of described substratum is 7-7.5.
Adopt the method for substratum cultivate agrocybe mushroom as above, it is characterized in that, comprise the following steps:
(1) carry out fermentation 1-2d for the ratio of 500:6 adds commercially available agriculture Citroen zx fermentation bed bacterium liquid in mass ratio in the medium, after turning, continue fermentation, stop fermentation when temperature reaches 55-60 DEG C, obtain the substratum fermented;
(3) substratum fermented obtained in step (1) is loaded in single port plastics bag, carry out tea tree mushroom inoculation, at room temperature cultivate 40-50d and eat up material in mycelia, carry out management of producing mushroom.
The concrete grammar of the management of producing mushroom described in step (2) is: after tea tree mushroom mycelia covers with bag, separate opened mouth, newspaper on sack upper cover, to ground and space water spray, improve relative air humidity and reach 85-90%, light control 500-1000Lux, temperature controls at 10-26 DEG C, bacterium bag surface mycelia overstrike patch after 5-7d, above adularescent hypha body, can gather after 8-12d.
The present invention compared with prior art, beneficial effect be embodied in following some:
1. the present invention selects the Peach sawdust after burin-in process and willow wood chip as one of main raw material of substratum, Peach sawdust after burin-in process and willow wood chip easily resolve into polysaccharide and monose, be easy to be absorbed by tea tree mushroom, camellia is the edible of Southwestern China area and medicinal plant, tea oil bran remaining after seed oil expression is containing the thick saponin of oil tea, inhibited to multiple bacterium in vitro, the present invention using itself and other nutritive ingredient according to a certain ratio as the substratum of tea tree mushroom, for its growth and breeding has entered to supply a good ecotope.
2. the various compositions needed for tea tree mushroom growth combine by the present invention according to a certain ratio, the water content of substratum is pressed built in 60-65%, adopt PH conditioning agent to be adjusted to 7.0-7.5 the pH value of substratum, be more conducive to the growth and breeding of tea tree mushroom, thus improve the output of tea tree mushroom.
3. the present invention adds a certain amount of agar strip in the medium, liquid nutrient medium is transformed into semisolid or solid medium, can obviously improve substratum ventilation problem, also be beneficial to the observation of tea tree mushroom growth and breeding.
Embodiment
Below in conjunction with embodiment, the present invention will be further described.
Embodiment 1
Improve a substratum for tea tree mushroom nutritive ingredient, described substratum is made up of the component comprising following weight part:
Peach wood chip 15,
Willow wood chip 20,
Camellia shell 10,
Analysis for soybean powder 1,
Potassium primary phosphate 0.5,
Camellia oil bran 3,
Magnesium sulfate 0.5
Agar strip 2,
Gypsum 1,
Defoamer 0.5,
PH conditioning agent,
Water surplus.
Described peach wood chip and willow wood chip are through the aging wood chip of 30d Exposure to Sunlight.
Described peach wood chip and the length of willow wood chip are 0.59cm.
Described camellia shell is pulverizing rear surface diameter is the solid particulate of 0.3cm.
Described water is the distilled water surplus after high-temperature boiling 15min.
Described agar strip containing water surplus amount be 5%, the water content of described substratum is 60%.
Described PH conditioning agent is the aqueous sodium hydroxide solution of 0.1mol/L, and the PH of described substratum is 7.
Adopt the method for substratum cultivate agrocybe mushroom as above, it is characterized in that, comprise the following steps:
(1) carry out fermentation 1d for the ratio of 500:6 adds commercially available agriculture Citroen zx fermentation bed bacterium liquid in mass ratio in the medium, after turning, continue fermentation, stop fermentation when temperature reaches 55 DEG C, obtain the substratum fermented;
(2) substratum fermented obtained in step (1) is loaded in single port plastics bag, carry out tea tree mushroom inoculation, at room temperature cultivate 40d and eat up material in mycelia, carry out management of producing mushroom.
The concrete grammar of the management of producing mushroom described in step (2) is: after tea tree mushroom mycelia covers with bag, separate opened mouth, newspaper on sack upper cover, to ground and space water spray, improve relative air humidity and reach 85%, light control 500Lux, temperature controls at 10 DEG C, bacterium bag surface mycelia overstrike patch after 5d, above adularescent hypha body, can gather after 8d.
Embodiment 1
Improve a substratum for tea tree mushroom nutritive ingredient, described substratum is made up of the component comprising following weight part:
Peach wood chip 20,
Willow wood chip 25,
Camellia shell 15,
Analysis for soybean powder 3,
Potassium primary phosphate 1.5,
Camellia oil bran 6,
Magnesium sulfate 1,
Agar strip 4,
Gypsum 3,
Defoamer 2,
PH conditioning agent,
Water surplus.
Described peach wood chip and willow wood chip are through the aging wood chip of 40d Exposure to Sunlight.
Described peach wood chip and the length of willow wood chip are 0.5-0.9cm.
Described camellia shell is pulverizing rear surface diameter is the solid particulate of 0.8cm.
Described water surplus is the distilled water surplus after high-temperature boiling 30min.
Described agar strip containing water surplus amount be 8%, the water content of described substratum is 65%.
Described PH conditioning agent is the aqueous sodium hydroxide solution of 0.3mol/L, and the PH of described substratum is 7.5.
Adopt the method for substratum cultivate agrocybe mushroom as above, it is characterized in that, comprise the following steps:
(1) carry out fermentation 2d for the ratio of 500:6 adds commercially available agriculture Citroen zx fermentation bed bacterium liquid in mass ratio in the medium, after turning, continue fermentation, stop fermentation when temperature reaches 60 DEG C, obtain the substratum fermented;
(2) substratum fermented obtained in step (1) is loaded in single port plastics bag, carry out tea tree mushroom inoculation, at room temperature cultivate 50d and eat up material in mycelia, carry out management of producing mushroom.
The concrete grammar of the management of producing mushroom described in step (2) is: after tea tree mushroom mycelia covers with bag, separate opened mouth, newspaper on sack upper cover, to ground and space water spray, improve relative air humidity and reach 90%, light control 1000Lux, temperature controls at 26 DEG C, bacterium bag surface mycelia overstrike patch after 7d, above adularescent hypha body, can gather after 12d.
Embodiment 1
Improve a substratum for tea tree mushroom nutritive ingredient, described substratum is made up of the component comprising following weight part:
Peach wood chip 18,
Willow wood chip,
Camellia shell 12,
Analysis for soybean powder 2,
Potassium primary phosphate 1.0,
Camellia oil bran 4.5,
Magnesium sulfate 0.8,
Agar strip 3,
Gypsum 2,
Defoamer 1,
PH conditioning agent,
Water surplus.
Described peach wood chip and willow wood chip are through the aging wood chip of 355d Exposure to Sunlight.
Described peach wood chip and the weight ratio of willow wood chip are 1:1.5.
Described peach wood chip and the length of willow wood chip are 0.7cm.
Described camellia shell is pulverizing rear surface diameter is the solid particulate of 0.5cm.
Described water surplus is the distilled water surplus after high-temperature boiling 20min.
Described agar strip containing water surplus amount be 6%, the water content of described substratum is 62%.
Described PH conditioning agent is the aqueous hydrochloric acid of 0.02mol/L, and the PH of described substratum is 7.2.
Adopt the method for substratum cultivate agrocybe mushroom as above, it is characterized in that, comprise the following steps:
(1) carry out fermentation 1d for the ratio of 500:6 adds commercially available agriculture Citroen zx fermentation bed bacterium liquid in mass ratio in the medium, after turning, continue fermentation, stop fermentation when temperature reaches 58 DEG C, obtain the substratum fermented;
(2) substratum fermented obtained in step (1) is loaded in single port plastics bag, carry out tea tree mushroom inoculation, at room temperature cultivate 45d and eat up material in mycelia, carry out management of producing mushroom.
The concrete grammar of the management of producing mushroom described in step (2) is: after tea tree mushroom mycelia covers with bag, separate opened mouth, newspaper on sack upper cover, to ground and space water spray, improve relative air humidity and reach 88%, light control 800Lux, temperature controls at 20 DEG C, bacterium bag surface mycelia overstrike patch after 6d, above adularescent hypha body, can gather after 10d.
Above-described embodiment is only the preferred embodiment of the present invention, and should not be construed as limitation of the invention, and those skilled in the art, according to announcement of the present invention, do not depart from improvement that scope makes and amendment all should within protection scope of the present invention.
Claims (10)
1. can improve a substratum for tea tree mushroom nutritive ingredient, it is characterized in that, described substratum is made up of the component comprising following weight part:
Peach wood chip 15-20,
Willow wood chip 20-25,
Camellia shell 10-15,
Analysis for soybean powder 1-3,
Potassium primary phosphate 0.5-1.5,
Camellia oil bran 3-6,
Magnesium sulfate 0.5-1,
Agar strip 2-4,
Gypsum 1-3,
Defoamer 0.5-2,
PH conditioning agent,
Water surplus.
2. a kind of substratum improving tea tree mushroom nutritive ingredient according to claim 1, is characterized in that, described peach wood chip and willow wood chip are through the aging wood chip of 30-40d Exposure to Sunlight.
3. a kind of substratum improving tea tree mushroom nutritive ingredient according to claim 1, is characterized in that, described peach wood chip and the weight ratio of willow wood chip are 1:1.5.
4. a kind of substratum improving tea tree mushroom nutritive ingredient according to claim 1, is characterized in that, described peach wood chip and the length of willow wood chip are 0.5-0.9cm.
5. a kind of substratum improving tea tree mushroom nutritive ingredient according to claim 1, is characterized in that, described camellia shell is pulverizing rear surface diameter is the solid particulate of 0.3-0.8cm.
6. a kind of substratum improving tea tree mushroom nutritive ingredient according to claim 1, is characterized in that, described water is the distilled water after high-temperature boiling 15-30min.
7. a kind of substratum improving tea tree mushroom nutritive ingredient according to claim 1, is characterized in that, the water content of described agar strip is 5-8%, and the water content of described substratum is 60-65%.
8. a kind of substratum improving tea tree mushroom nutritive ingredient according to claim 1, it is characterized in that, described PH conditioning agent is selected from the one in the aqueous sodium hydroxide solution of 0.1-0.3mol/L or the aqueous hydrochloric acid of 0.02-0.05mol/L, and the PH of described substratum is 7-7.5.
9. adopt as arbitrary in claim 1-8 as described in the method for substratum cultivate agrocybe mushroom, it is characterized in that, comprise the following steps:
(1) carry out fermentation 1-2d for the ratio of 500:6 adds commercially available agriculture Citroen zx fermentation bed bacterium liquid in mass ratio in the medium, after turning, continue fermentation, stop fermentation when temperature reaches 55-60 DEG C, obtain the substratum fermented;
(2) substratum fermented obtained in step (1) is loaded in single port plastics bag, carry out tea tree mushroom inoculation, at room temperature cultivate 40-50d and eat up material in mycelia, carry out management of producing mushroom.
10. cultural method according to claim 9, it is characterized in that the concrete grammar of the management of producing mushroom described in step (2) is: after tea tree mushroom mycelia covers with bag, separate opened mouth, newspaper on sack upper cover, to ground and space water spray, improve relative air humidity and reach 85-90%, light control 500-1000Lux, temperature controls bacterium bag surface mycelia overstrike patch after 10-26 DEG C, 5-7d, adularescent hypha body above, can gather after 8-12d.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104671993A (en) * | 2015-01-30 | 2015-06-03 | 吴晖 | Special culture medium for shii-take |
| CN104987179A (en) * | 2015-06-16 | 2015-10-21 | 王金生 | Agrocybe cylindracea cultivation medium |
| CN106416746A (en) * | 2016-08-31 | 2017-02-22 | 成都玉麟农业开发有限公司 | Cultivation method capable of increasing nutrition content of agrocybe cylindracea |
| CN107176878A (en) * | 2017-06-15 | 2017-09-19 | 柳城新天地生态农业发展有限公司 | The method for improving elegant precious mushroom cultivating rate |
| CN108575564A (en) * | 2018-06-19 | 2018-09-28 | 贵州省贵福菌业发展有限公司 | a kind of oil tea mushroom culture medium |
| CN111758490A (en) * | 2020-08-17 | 2020-10-13 | 湖南和平生物科技有限公司 | Agrocybe cylindracea cultivation method |
| CN112997805A (en) * | 2021-03-31 | 2021-06-22 | 广东雄达实业发展有限公司 | Agrocybe cylindracea cultivation method taking stem raw material of camellia fruit tree as culture medium |
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| CN1745617A (en) * | 2004-09-06 | 2006-03-15 | 黄永红 | Tea mushroom cultivation with non-broken tea seed shells |
| CN103304341A (en) * | 2013-07-16 | 2013-09-18 | 王龙 | Agrocybe cylindracea culture medium and preparation method thereof |
| CN103787700A (en) * | 2014-01-27 | 2014-05-14 | 韦承梭 | Culture medium for agrocybe cylindracea and cultivation method of agrocybe cylindracea |
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2014
- 2014-10-20 CN CN201410556228.9A patent/CN104311329A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1745617A (en) * | 2004-09-06 | 2006-03-15 | 黄永红 | Tea mushroom cultivation with non-broken tea seed shells |
| CN103304341A (en) * | 2013-07-16 | 2013-09-18 | 王龙 | Agrocybe cylindracea culture medium and preparation method thereof |
| CN103787700A (en) * | 2014-01-27 | 2014-05-14 | 韦承梭 | Culture medium for agrocybe cylindracea and cultivation method of agrocybe cylindracea |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104671993A (en) * | 2015-01-30 | 2015-06-03 | 吴晖 | Special culture medium for shii-take |
| CN104987179A (en) * | 2015-06-16 | 2015-10-21 | 王金生 | Agrocybe cylindracea cultivation medium |
| CN106416746A (en) * | 2016-08-31 | 2017-02-22 | 成都玉麟农业开发有限公司 | Cultivation method capable of increasing nutrition content of agrocybe cylindracea |
| CN107176878A (en) * | 2017-06-15 | 2017-09-19 | 柳城新天地生态农业发展有限公司 | The method for improving elegant precious mushroom cultivating rate |
| CN108575564A (en) * | 2018-06-19 | 2018-09-28 | 贵州省贵福菌业发展有限公司 | a kind of oil tea mushroom culture medium |
| CN111758490A (en) * | 2020-08-17 | 2020-10-13 | 湖南和平生物科技有限公司 | Agrocybe cylindracea cultivation method |
| CN112997805A (en) * | 2021-03-31 | 2021-06-22 | 广东雄达实业发展有限公司 | Agrocybe cylindracea cultivation method taking stem raw material of camellia fruit tree as culture medium |
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