CN106133524A - 通过质谱细胞术对组织样品的多重成像 - Google Patents

通过质谱细胞术对组织样品的多重成像 Download PDF

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Publication number
CN106133524A
CN106133524A CN201580018145.7A CN201580018145A CN106133524A CN 106133524 A CN106133524 A CN 106133524A CN 201580018145 A CN201580018145 A CN 201580018145A CN 106133524 A CN106133524 A CN 106133524A
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CN
China
Prior art keywords
tissue sample
cell
labelling
imaging
plume
Prior art date
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CN201580018145.7A
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English (en)
Chinese (zh)
Inventor
贝恩德·博登米勒
浩·王
德特勒夫·巩特尔
夏洛特·吉森
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eidgenoessische Technische Hochschule Zurich ETHZ
Universitaet Zuerich
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Eidgenoessische Technische Hochschule Zurich ETHZ
Universitaet Zuerich
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Application filed by Eidgenoessische Technische Hochschule Zurich ETHZ, Universitaet Zuerich filed Critical Eidgenoessische Technische Hochschule Zurich ETHZ
Publication of CN106133524A publication Critical patent/CN106133524A/zh
Pending legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/60—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances involving radioactive labelled substances
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/0004—Imaging particle spectrometry
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/02—Details
    • H01J49/10—Ion sources; Ion guns
    • H01J49/105—Ion sources; Ion guns using high-frequency excitation, e.g. microwave excitation, Inductively Coupled Plasma [ICP]
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/26—Mass spectrometers or separator tubes
    • H01J49/34—Dynamic spectrometers
    • H01J49/40—Time-of-flight spectrometers

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Electron Tubes For Measurement (AREA)
CN201580018145.7A 2014-02-28 2015-02-27 通过质谱细胞术对组织样品的多重成像 Pending CN106133524A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB201403625A GB201403625D0 (en) 2014-02-28 2014-02-28 Multiplexed imaging of tissue samples by mass cytometry
GB1403625.5 2014-02-28
PCT/EP2015/054196 WO2015128490A1 (en) 2014-02-28 2015-02-27 Multiplexed imaging of tissue samples by mass cytometry with subcellular resolution

Publications (1)

Publication Number Publication Date
CN106133524A true CN106133524A (zh) 2016-11-16

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Family Applications (1)

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CN201580018145.7A Pending CN106133524A (zh) 2014-02-28 2015-02-27 通过质谱细胞术对组织样品的多重成像

Country Status (6)

Country Link
EP (1) EP3111464A1 (ja)
JP (1) JP2017512985A (ja)
CN (1) CN106133524A (ja)
CA (1) CA2940553A1 (ja)
GB (1) GB201403625D0 (ja)
WO (1) WO2015128490A1 (ja)

Cited By (10)

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CN107505252A (zh) * 2017-08-14 2017-12-22 浙江普罗亭健康科技有限公司 一种用于质谱流式细胞技术的新型Barcoding 89Y试剂
CN108918369A (zh) * 2018-06-27 2018-11-30 太原理工大学 一种用于检测贴壁细胞浓度的微流控芯片及其制备方法
CN108918370A (zh) * 2018-06-27 2018-11-30 太原理工大学 一种用于检测贴壁细胞浓度的方法及其装置
CN109919924A (zh) * 2019-02-28 2019-06-21 江南大学 一种适用于大批量he染色图片细胞数字化处理的方法
CN110412286A (zh) * 2019-07-11 2019-11-05 上海宸安生物科技有限公司 一种利用质谱流式***对肿瘤样本进行单细胞检测的方法
CN110412287A (zh) * 2019-07-11 2019-11-05 上海宸安生物科技有限公司 一种基于单细胞的免疫细胞分型定量分析方法
CN110763752A (zh) * 2019-11-27 2020-02-07 清华大学 单细胞萃取电喷雾质谱分析***和方法
CN112970092A (zh) * 2018-09-10 2021-06-15 富鲁达加拿大公司 高速调制样本成像设备和方法
CN112997064A (zh) * 2018-09-10 2021-06-18 富鲁达加拿大公司 用于成像质谱分析法的基于融合的参考颗粒的归一化
CN113678227A (zh) * 2018-06-18 2021-11-19 富鲁达加拿大公司 高分辨率成像设备和方法

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3043891B1 (en) 2013-09-13 2019-01-16 The Board of Trustees of The Leland Stanford Junior University Multiplexed imaging of tissues using mass tags and secondary ion mass spectrometry
EP3274719B1 (en) 2015-03-25 2021-02-24 The Board of Trustees of the Leland Stanford Junior University Single cell analysis using secondary ion mass spectrometry
US9797879B2 (en) 2015-04-23 2017-10-24 The Board Of Trustees Of The Leland Stanford Junior University Method for multiplexed sample analysis by photoionizing secondary sputtered neutrals
GB201513167D0 (en) 2015-07-27 2015-09-09 Thermo Fisher Scient Bremen Elemental analysis of organic samples
CN110050071A (zh) 2016-12-09 2019-07-23 乌尔蒂维尤股份有限公司 用于使用标记的核酸成像剂进行多路复用成像的改进方法
CN117551741A (zh) 2017-03-31 2024-02-13 乌尔蒂维尤股份有限公司 Dna-抗原交换和扩增
AU2018252185A1 (en) 2017-04-13 2019-10-31 European Molecular Biology Laboratory Single-cell imaging mass spectrometry
CN107796748B (zh) * 2017-09-28 2020-06-26 上海交通大学 一种用于单细胞质谱流式细胞术的检测方法
JP7226538B2 (ja) * 2019-05-17 2023-02-21 株式会社島津製作所 細胞外小胞の検出方法
US20220336058A1 (en) * 2019-09-25 2022-10-20 Cedars-Sinai Medical Center Analysis methods for multiplex tissue imaging including imaging mass cytometry data
CN115066609A (zh) 2020-01-17 2022-09-16 苏黎世大学 肿瘤样品的单细胞病理分析
JP7375640B2 (ja) * 2020-03-23 2023-11-08 株式会社島津製作所 イメージング質量分析システム、及び、イメージング質量分析を利用した分析方法
CN117110175A (zh) * 2023-09-02 2023-11-24 上海凯来仪器有限公司 一种飞秒激光剥蚀质谱流式一体机及其使用方法

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JP6230693B2 (ja) * 2013-04-17 2017-11-15 フリューダイム カナダ インコーポレイテッド マス・サイトメトリーのための試料分析

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CHARLOTTE GIESEN ET AL.: "Multiplexed Immunohistochemical Detection of Tumor Markers in Breast Cancer Tissue Using Laser Ablation Inductively Coupled Plasma Mass Spectrometry", 《ANAL.CHEM.》 *
HAOA.O.WANG ET AL.: "Fast Chemical Imaging at High Spatial Resolution by Laser Ablation Inductively Coupled Plasma Mass Spectrometry", 《ANAL.CHEM.》 *
J.SABINE BECKER ET AL.: "BIOIMAGING OF METALS BY LASER ABLATION INDUCTIVELY COUPLED PLASMA MASS SPECTROMETRY(LA-ICP-MS）", 《MASS SPECTROMETRY REVIEWS》 *

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107505252A (zh) * 2017-08-14 2017-12-22 浙江普罗亭健康科技有限公司 一种用于质谱流式细胞技术的新型Barcoding 89Y试剂
CN113678227A (zh) * 2018-06-18 2021-11-19 富鲁达加拿大公司 高分辨率成像设备和方法
CN108918369A (zh) * 2018-06-27 2018-11-30 太原理工大学 一种用于检测贴壁细胞浓度的微流控芯片及其制备方法
CN108918370A (zh) * 2018-06-27 2018-11-30 太原理工大学 一种用于检测贴壁细胞浓度的方法及其装置
CN108918369B (zh) * 2018-06-27 2020-10-09 太原理工大学 一种用于检测贴壁细胞浓度的微流控芯片及其制备方法
CN108918370B (zh) * 2018-06-27 2020-10-09 太原理工大学 一种用于检测贴壁细胞浓度的方法及其装置
CN112970092A (zh) * 2018-09-10 2021-06-15 富鲁达加拿大公司 高速调制样本成像设备和方法
CN112997064A (zh) * 2018-09-10 2021-06-18 富鲁达加拿大公司 用于成像质谱分析法的基于融合的参考颗粒的归一化
CN109919924A (zh) * 2019-02-28 2019-06-21 江南大学 一种适用于大批量he染色图片细胞数字化处理的方法
CN110412286A (zh) * 2019-07-11 2019-11-05 上海宸安生物科技有限公司 一种利用质谱流式***对肿瘤样本进行单细胞检测的方法
CN110412287A (zh) * 2019-07-11 2019-11-05 上海宸安生物科技有限公司 一种基于单细胞的免疫细胞分型定量分析方法
CN110763752A (zh) * 2019-11-27 2020-02-07 清华大学 单细胞萃取电喷雾质谱分析***和方法

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Publication number Publication date
WO2015128490A1 (en) 2015-09-03
EP3111464A1 (en) 2017-01-04
GB201403625D0 (en) 2014-04-16
JP2017512985A (ja) 2017-05-25
CA2940553A1 (en) 2015-09-03

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Application publication date: 20161116