CN106116893A

CN106116893A - A kind of Fructus Fragariae Ananssae culture matrix of air permeable humidity retaining

Info

Publication number: CN106116893A
Application number: CN201610499530.4A
Authority: CN
Inventors: 张殿兴; 火明勤
Original assignee: Fuyang City Dian Xing Agricultural Science And Technology Co Ltd
Current assignee: Fuyang City Dian Xing Agricultural Science And Technology Co Ltd
Priority date: 2016-06-28
Filing date: 2016-06-28
Publication date: 2016-11-16

Abstract

The invention discloses the Fructus Fragariae Ananssae culture matrix of a kind of air permeable humidity retaining, be prepared by the raw materials in: kelp residue 22 25, acidic cellulase 1.0 1.2, rice chaff ash 16 20, sodium alginate 23, ammonium molybdate 56, lentinan 23, attapulgite 8 10, sunflower plate granulated slag 36 38, Periostracum Serpentis waste material 33 35, pepsin preparation 23,1 (3 dimethylamino-propyl) 3 ethyl-carbodiimide hydrochloride 1.4 1.8, N N-Hydroxysuccinimide 0.6 0.7,4% sodium chloride solution are appropriate, 0.15mol/L citric acid solution is appropriate, water is appropriate；The culture matrix of the present invention utilizes enzymic degradation plant organism, promote the fast decoupled of cellulose, coordinate the use of attapulgite etc., sponginess and the breathability of substrate is improve while increasing its content adsorption area, enhancing the absorption to moisture and holding to stay, preferable physicochemical property and the savings of comprehensive nutrient meet the industry development needs of presentization Fructus Fragariae Ananssae substrate culture.

Description

A kind of Fructus Fragariae Ananssae culture matrix of air permeable humidity retaining

Technical field

The present invention relates to nutrient matrix technical field, particularly relate to the Fructus Fragariae Ananssae culture matrix of a kind of air permeable humidity retaining.

Background technology

Substrate culture is a kind of important cultural method in the current agriculture of China, woods, flower planting industry, is that one passes through base Matter absorbs nutritional solution and a kind of Soilless Culture Methods of oxygen, owing to it is low to soil environment requirement, equipment simple, water-saving saves fertilizer etc. Advantage, is adopted application by vast plant development person.And continuous along with the development of China's plant husbandry and cultivated area Expanding, the usage amount of cultivation matrix the most constantly increases, and traditional uses the single raw material such as the peat composed of rotten mosses, Vermiculitum or be simply mixed preparation Substrate can not meet the demand of plantation, and the universal nutrition of present stage substrate simultaneously is single, causes sending out of plant in cultivation Bud rate is low, survival rate is low, the physical instability of substrate during simultaneously using, and water absorption is poor, easily subside, surcharge Low, it is impossible to realize higher using value for specific environment, affect the growth of plantation economy benefit.

Collagen protein, as a kind of biomacromolecule, has good pore structure after being modified, the most raw Thing degradability, mechanical strength and biocompatibility, have good cell growth interface, can become a kind of cultivation matrix and build In quality raw materials.China Serpentis aboundresources simultaneously, owing to the decorative pattern of Periostracum Serpentis outward appearance is the most gorgeous, pattern is unique, is used for making The artware of various finenesses, but due to the backwardness of Periostracum Serpentis Technology, the course of processing creates a large amount of waste material, account for complete The 20-30% of Periostracum Serpentis, if not being effectively treated, not only polluting environment, also wasting substantial amounts of raw material, containing substantial amounts of in Periostracum Serpentis Collagen protein, this also applies for it and provides cheap raw material basis in substrate preparation.

The application for a patent for invention of Publication No. CN1702061A discloses the full nutrient matrix of a kind of botanical seedling culturing and cultivation, This nutrient matrix use weight ratio be the peat of 50-70%, the perlite of 20-25%, the Vermiculitum of 10-15 and active substance, Nutrient substance mixes, and has more comprehensively nutrients structure, but the cultivation matrix of this disclosure of the invention is due to size not One, easily cause sedimentation after watering, cause the blocking of air in substrate, the crude fiber content in substrate is low simultaneously, needs extra battalion Support the interpolation of material, improve production cost, and not for the additional use value of the real needs raising substrate cultivated, limit Make the scale application of product.

Summary of the invention

The object of the invention is contemplated to make up the defect of prior art, it is provided that the Fructus Fragariae Ananssae culture matrix of a kind of air permeable humidity retaining.

The present invention is achieved by the following technical solutions:

The Fructus Fragariae Ananssae culture matrix of a kind of air permeable humidity retaining, is prepared by the raw materials in: kelp residue 22-25, acid fibre Dimension element enzyme 1.0-1.2, rice chaff ash 16-20, sodium alginate 2-3, ammonium molybdate 5-6, lentinan 2-3, attapulgite 8-10, Xiang Certain herbaceous plants with big flowers dish granulated slag 36-38, Periostracum Serpentis waste material 33-35, pepsin preparation 2-3,1-(3-dimethylamino-propyl)-3-ethyl carbodiimide Hydrochlorate 1.4-1.8, N-hydroxy-succinamide 0.6-0.7,4% sodium chloride solution are appropriate, 0.15mol/L citric acid solution is fitted Amount, water are appropriate.

Specifically comprising the following steps that of the Fructus Fragariae Ananssae culture matrix preparation method of described air permeable humidity retaining

(1) kelp residue, acidic cellulase, rice chaff ash mixing are placed in fermentation vat, add water to ferment heap maximum water holding capacity 78-82%, ferment 24-25 days, by fermentation material squeeze and filter after having fermented, obtain fermentation liquid and ferment slag is standby, simultaneously will be to Day certain herbaceous plants with big flowers dish cuts off, it is standby to pulverize as smalls；

(2) 4% sodium chloride solution is added by 1:16-20g/mL solid-to-liquid ratio after being shredded by Periostracum Serpentis waste material, after soaking 6-8 hour Centrifugal 12-15 minute with 5800-6200 rev/min, take precipitation clear water clean and at 30-32 DEG C dry, then will precipitation with 0.15mol/L citric acid solution presses 1:8-10g/mL solid-to-liquid ratio mix homogeneously, adds pepsin preparation, is heated to 30-32 DEG C, Insulation is extracted 18-20 hour, completes post-heating and is warming up to 85-95 DEG C, is incubated 12 minutes, then is centrifuged with 5000-5500 rev/min 10-12 minute, collect the supernatant, obtain collagen solution standby；

(3) in step 1 gained fermentation liquid, add sodium alginate, ammonium molybdate, lentinan, be heated to 58-60 DEG C, reaction After 25-30 minute cool down, then with step 1 gained ferment slag, sunflower plate granulated slag, attapulgite mixing for standby use；

(4) step 2 gained collagen solution is joined in step 3 gained material, is placed under 4-5 DEG C of low temperature environment, After stirring 5-10 minute add 1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride, N-hydroxy-succinamide with And other leftover materials, cross-linking reaction 8-10 hour, after completing, material is warming up to 28-30 DEG C of drying, obtains substrate of the present invention.

The invention have the advantage that

Substrate of the present invention is by the extraction to Periostracum Serpentis, and by having reduced immunogenicity, the collagen protein of biodegradability should Use in the preparation of cultivation matrix, utilize the mechanical performance of cross-linking modified raising albumen, it is achieved its support in substrate builds Effect, can not only provide certain support and growth interface, the collagenous fiber bundle phase within collagen protein simultaneously for seed seedling Be staggered porous network structure mutually that formed, is also beneficial to beneficial microorganism and carries and the extension of root cell, and is It provides sufficient oxygen and nutrient substance, promotes the growth of plant, and culture matrix plays fixation, convenient transport.

The culture matrix of the present invention utilizes enzymic degradation plant organism, promotes the fast decoupled of cellulose, coordinates concavo-convex The use of rod powder etc., improves sponginess and the breathability of substrate while increasing its content adsorption area, and it is right to enhance The absorption of moisture and holding is stayed, and preferable physicochemical property and the savings of comprehensive nutrient meet the industry of presentization Fructus Fragariae Ananssae substrate culture Development need.

Detailed description of the invention

The Fructus Fragariae Ananssae culture matrix of a kind of air permeable humidity retaining, is made up of the raw material of following weight portion (kg): kelp residue 22, acid fibre Dimension element enzyme 1.0, rice chaff ash 16, sodium alginate 2, ammonium molybdate 5, lentinan 2, attapulgite 8, sunflower plate granulated slag 36, Periostracum Serpentis Waste material 33, pepsin preparation 2,1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride 1.4, N hydroxysuccinimidyl acyl are sub- Amine 0.6,4% sodium chloride solution is appropriate, 0.15mol/L citric acid solution is appropriate, water is appropriate.

(1) kelp residue, acidic cellulase, rice chaff ash mixing are placed in fermentation vat, add water to ferment heap maximum water holding capacity 78%, ferment 24 days, by fermentation material squeeze and filter after having fermented, obtain fermentation liquid and ferment slag is standby, simultaneously by sunflower plate Cut off, pulverize as smalls standby；

(2) add 4% sodium chloride solution by 1:16g/mL solid-to-liquid ratio after being shredded by Periostracum Serpentis waste material, after soaking 6 hours with 5800 revs/min are centrifuged 12 minutes, take precipitation clear water and clean and dry at 30 DEG C, then will precipitation and 0.15mol/L citric acid Solution presses 1:8g/mL solid-to-liquid ratio mix homogeneously, adds pepsin preparation, is heated to 30 DEG C, and insulation is extracted 18 hours, after completing It is heated to 85 DEG C, is incubated 12 minutes, then be centrifuged 10 minutes with 5000 revs/min, collect the supernatant, obtain collagen solution Standby；

(3) in step 1 gained fermentation liquid, add sodium alginate, ammonium molybdate, lentinan, be heated to 58 DEG C, react 25 After minute cool down, then with step 1 gained ferment slag, sunflower plate granulated slag, attapulgite mixing for standby use；

(4) step 2 gained collagen solution is joined in step 3 gained material, be placed under 4 DEG C of low temperature environments, stir After mixing 5 minutes add 1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride, N N-Hydroxysuccinimide and other Leftover materials, cross-linking reaction 8 hours, after completing, material is warming up to 28 DEG C of drying, obtains substrate of the present invention.

In order to further illustrate the using value of the present invention, inventor have chosen respectively substrate of the present invention and commercially available commonly Culture matrix carries out the cultivating and growing of Fructus Fragariae Ananssae, and during plantation, management method is identical, and during plantation, the physical and chemical index to substrate is carried out Detection, result is as follows:

	Full nitrogen (%)	Full phosphorus (%)	Full potassium (%)	Total porosity (%)	Saturation moisture content (%)
						Substrate of the present invention	1.091	0.10	0.46	90.34	861
Commercially available generic media	0.798	0.09	0.43	85.10	456

Substrate the most of the present invention plantation gained Yield of Strawberry relatively generic media improves more than 10%, it can be seen that the present invention Substrate relatively generic media is the most sufficient, simultaneously stable in physicochemical property, and structure is excellent, has preferable prospect of the application.

Claims

1. the Fructus Fragariae Ananssae culture matrix of an air permeable humidity retaining, it is characterised in that be prepared by the raw materials in: kelp residue 22- 25, acidic cellulase 1.0-1.2, rice chaff ash 16-20, sodium alginate 2-3, ammonium molybdate 5-6, lentinan 2-3, attapulgite 8-10, sunflower plate granulated slag 36-38, Periostracum Serpentis waste material 33-35, pepsin preparation 2-3,1-(3-dimethylamino-propyl)-3-second Base carbodiimide hydrochloride 1.4-1.8, N-hydroxy-succinamide 0.6-0.7,4% sodium chloride solution are appropriate, 0.15mol/L lemon Lemon acid solution is appropriate, water is appropriate.

2. according to the Fructus Fragariae Ananssae culture matrix of the air permeable humidity retaining described in claims 1, it is characterised in that the concrete step of preparation method Rapid as follows:

(1) kelp residue, acidic cellulase, rice chaff ash mixing are placed in fermentation vat, add water to ferment heap maximum water holding capacity 78-82%, ferments 24-25 days, by fermentation material squeeze and filter after having fermented, obtains fermentation liquid and ferment slag is standby, simultaneously by Helianthi Dish cuts off, it is standby to pulverize as smalls；

(2) add 4% sodium chloride solution by 1:16-20g/mL solid-to-liquid ratio after being shredded by Periostracum Serpentis waste material, after soaking 6-8 hour with 5800-6200 rev/min is centrifugal 12-15 minute, take precipitation clear water clean and at 30-32 DEG C dry, then will precipitation with 0.15mol/L citric acid solution presses 1:8-10g/mL solid-to-liquid ratio mix homogeneously, adds pepsin preparation, is heated to 30-32 DEG C, Insulation is extracted 18-20 hour, completes post-heating and is warming up to 85-95 DEG C, is incubated 12 minutes, then is centrifuged with 5000-5500 rev/min 10-12 minute, collect the supernatant, obtain collagen solution standby；

(3) in step 1 gained fermentation liquid, add sodium alginate, ammonium molybdate, lentinan, be heated to 58-60 DEG C, react 25- After 30 minutes cool down, then with step 1 gained ferment slag, sunflower plate granulated slag, attapulgite mixing for standby use；

(4) step 2 gained collagen solution is joined in step 3 gained material, be placed under 4-5 DEG C of low temperature environment, stirring After 5-10 minute add 1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride, N-hydroxy-succinamide and its His leftover materials, cross-linking reaction 8-10 hour, after completing, material is warming up to 28-30 DEG C of drying, obtains substrate of the present invention.