CN106107957A - A kind of mangosteen skin gingerol antioxidant and preparation method thereof - Google Patents
A kind of mangosteen skin gingerol antioxidant and preparation method thereof Download PDFInfo
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- CN106107957A CN106107957A CN201610372045.0A CN201610372045A CN106107957A CN 106107957 A CN106107957 A CN 106107957A CN 201610372045 A CN201610372045 A CN 201610372045A CN 106107957 A CN106107957 A CN 106107957A
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- weight portion
- mangosteen skin
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- extract
- gingerol
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- 240000006053 Garcinia mangostana Species 0.000 title claims abstract description 49
- 235000017048 Garcinia mangostana Nutrition 0.000 title claims abstract description 49
- NLDDIKRKFXEWBK-AWEZNQCLSA-N gingerol Chemical compound CCCCC[C@H](O)CC(=O)CCC1=CC=C(O)C(OC)=C1 NLDDIKRKFXEWBK-AWEZNQCLSA-N 0.000 title claims abstract description 26
- JZLXEKNVCWMYHI-UHFFFAOYSA-N gingerol Natural products CCCCC(O)CC(=O)CCC1=CC=C(O)C(OC)=C1 JZLXEKNVCWMYHI-UHFFFAOYSA-N 0.000 title claims abstract description 26
- 235000002780 gingerol Nutrition 0.000 title claims abstract description 26
- 239000003963 antioxidant agent Substances 0.000 title claims abstract description 19
- 230000003078 antioxidant effect Effects 0.000 title claims abstract description 19
- 235000006708 antioxidants Nutrition 0.000 title claims abstract description 19
- 238000002360 preparation method Methods 0.000 title claims abstract description 9
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 claims abstract description 18
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract description 18
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 16
- 239000000843 powder Substances 0.000 claims abstract description 16
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims abstract description 9
- 229930003268 Vitamin C Natural products 0.000 claims abstract description 9
- 229930003427 Vitamin E Natural products 0.000 claims abstract description 9
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims abstract description 9
- 235000019154 vitamin C Nutrition 0.000 claims abstract description 9
- 239000011718 vitamin C Substances 0.000 claims abstract description 9
- 235000019165 vitamin E Nutrition 0.000 claims abstract description 9
- 229940046009 vitamin E Drugs 0.000 claims abstract description 9
- 239000011709 vitamin E Substances 0.000 claims abstract description 9
- 210000000582 semen Anatomy 0.000 claims abstract description 8
- 239000011780 sodium chloride Substances 0.000 claims abstract description 8
- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 claims abstract description 6
- FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 claims abstract description 6
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 claims abstract description 6
- 235000019155 vitamin A Nutrition 0.000 claims abstract description 6
- 239000011719 vitamin A Substances 0.000 claims abstract description 6
- 229940045997 vitamin a Drugs 0.000 claims abstract description 6
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 24
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 24
- 239000003480 eluent Substances 0.000 claims description 24
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 16
- 238000001035 drying Methods 0.000 claims description 14
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 claims description 14
- 229920002538 Polyethylene Glycol 20000 Polymers 0.000 claims description 8
- 238000004440 column chromatography Methods 0.000 claims description 8
- 239000003208 petroleum Substances 0.000 claims description 8
- 238000001953 recrystallisation Methods 0.000 claims description 8
- 238000010992 reflux Methods 0.000 claims description 8
- 239000003463 adsorbent Substances 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 238000000605 extraction Methods 0.000 claims description 4
- 238000010936 aqueous wash Methods 0.000 claims description 3
- 238000000034 method Methods 0.000 claims description 2
- 239000004952 Polyamide Substances 0.000 claims 1
- 150000001412 amines Chemical class 0.000 claims 1
- 238000004458 analytical method Methods 0.000 claims 1
- 229910002114 biscuit porcelain Inorganic materials 0.000 claims 1
- 229920002647 polyamide Polymers 0.000 claims 1
- 230000003064 anti-oxidating effect Effects 0.000 abstract description 3
- 230000000259 anti-tumor effect Effects 0.000 abstract description 2
- 239000008280 blood Substances 0.000 abstract description 2
- 210000004369 blood Anatomy 0.000 abstract description 2
- 230000002708 enhancing effect Effects 0.000 abstract description 2
- 239000000463 material Substances 0.000 abstract description 2
- 231100000957 no side effect Toxicity 0.000 abstract description 2
- 230000000050 nutritive effect Effects 0.000 abstract description 2
- 230000010100 anticoagulation Effects 0.000 abstract 1
- 239000000126 substance Substances 0.000 description 5
- 239000000654 additive Substances 0.000 description 4
- 230000000996 additive effect Effects 0.000 description 4
- 235000019261 food antioxidant Nutrition 0.000 description 4
- 239000012141 concentrate Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000023555 blood coagulation Effects 0.000 description 1
- 229910001424 calcium ion Inorganic materials 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000004118 muscle contraction Effects 0.000 description 1
- 230000003387 muscular Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000036542 oxidative stress Effects 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 235000020795 whole food diet Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Medicines Containing Plant Substances (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses a kind of mangosteen skin gingerol antioxidant and preparation method thereof.The mangosteen skin gingerol antioxidant of the present invention includes the component of following weight portion: mangosteen skin extract 20 30 weight portion, gingerol 35 weight portion, sodium chloride 0.01 0.02 weight portion, vitamin A 0.1 0.2 weight portion, vitamin E 0.1 0.5 weight portion and vitamin C 38 weight portion, Semen phaseoli radiati powder 0.1 0.2 weight portion, Thallus Porphyrae powder 0.01 0.02 weight portion.The present invention draws materials natural, is of high nutritive value, and has no side effect, it is possible to anticoagulation and blood fat reducing, enhancing immunocyte, antitumor, has good antioxidation, applied range.
Description
Technical field
The invention belongs to technical field of food additives, be specifically related to a kind of mangosteen skin gingerol antioxidant and system thereof
Preparation Method.
Background technology
Existing food oxydating resistance additive mainly has chemical food antioxidant and wholefood antioxidation to add
The big class of agent two, wherein, chemical food antioxidant is respond well, but there is the shortcomings such as toxic and side effects in various degree,
Health is caused damage by life-time service or excessive amount.Artificial chemistry food oxydating resistance additive refers to use artificial chemosynthesis
Obtained by method.Meanwhile, substantial amounts of research report is pointed out, almost all of chemical food antioxidant all can not
Thering is provided nutrient substance to human body, some chemical food antioxidant even can be detrimental to health.
Summary of the invention
It is an object of the invention to overcome prior art defect, it is provided that a kind of mangosteen skin gingerol antioxidant.
Another object of the present invention is to provide the preparation method of this mangosteen skin gingerol antioxidant.
Technical scheme is as follows:
A kind of mangosteen skin gingerol antioxidant, including the component of following weight portion:
Mangosteen skin extract 20-30 weight portion
Gingerol 3-5 weight portion
Sodium chloride 0.01-0.02 weight portion
Vitamin A 0.1-0.2 weight portion
Vitamin E 0.1-0.5 weight portion
Vitamin C 3-8 weight portion
Semen phaseoli radiati powder 0.1-0.2 weight portion.
Thallus Porphyrae powder 0.01-0.02 weight portion
Another technical scheme of the present invention is as follows:
The preparation method of a kind of above-mentioned mangosteen skin gingerol antioxidant, comprises the steps:
(1) by mangosteen skin successively through ethanol water hot reflux extraction, petroleum ether extraction, PEG 20000 chromatographic column chromatography,
Silon chromatography, eluant ethanol recrystallization and dried, prepare described mangosteen skin extract；
(2) by above-mentioned mangosteen skin extract 20-30 weight portion, gingerol 3-5 weight portion, sodium chloride 0.01-0.02 weight portion, dimension life
Element A0.1-0.2 weight portion, vitamin E 0.1-0.5 weight portion and vitamin C 3-8 weight portion, Semen phaseoli radiati powder 0.1-0.2 weight portion,
Thallus Porphyrae powder 0.01-0.02 weight portion mixing and stirring, the most i.e. obtains described mangosteen skin gingerol antioxidant.
In a preferred embodiment of the invention, described step (1) comprises the steps:
A, extract adding ethanol water after above-mentioned mangosteen skin drying and crushing 40-60 minute in 50-60 DEG C of hot reflux, and mistake
Filter, obtains lixiviating solution；
B, above-mentioned lixiviating solution petroleum ether is extracted, obtain extract；
C, above-mentioned extract PEG 20000 post is carried out column chromatography, then be the ethanol aqueous wash of 5-10% by concentration
De-, obtain first order eluent；
D, above-mentioned first order eluent Silon is chromatographed as adsorbent, then use aqueous acetic acid eluting, obtain
Two grades of eluents；
E, above-mentioned second level eluent ethanol is carried out recrystallization, after drying, obtain described mangosteen skin extract.In the present invention
A preferred embodiment in, in step a of described step (1), the concentration of ethanol water is 10-20%, and this ethanol is water-soluble
Liquid weight is 4-5 times of the weight of the mangosteen skin after drying and crushing.
In a preferred embodiment of the invention, in step d of described step (1), described Silon is 300
Mesh, the concentration of described aqueous acetic acid is 1-3%.
The invention has the beneficial effects as follows:
1, containing mangosteen skin in the antioxidant of the present invention, draw materials natural, be of high nutritive value, have no side effect, it is possible to be anti-
Blood coagulation and blood fat reducing, enhancing immunocyte, antitumor, have good antioxidation, applied range；
2, important polyphenoils and membrane stabilizer in the vitamin E in the antioxidant of the present invention is cell membrane, maintain
The normal mechanism of muscular tissue and metabolism, particularly the energy supply during muscle contraction have with calcium ion picked-up and release
Important effect；
3, the vitamin C in the antioxidant of the present invention can obviously reduce exercise induced oxidative stress.
Detailed description of the invention
Below by way of detailed description of the invention technical scheme it is further detailed and describes.
Embodiment 1
(1) mangosteen skin extract is prepared:
A, 4kg10% ethanol water will be added after 1kg mangosteen skin drying and crushing, and extract 40 minutes in 50 DEG C of hot refluxs, and filter
Concentrate, obtain lixiviating solution；
B, above-mentioned lixiviating solution petroleum ether is extracted, obtain extract；
C, above-mentioned extract PEG 20000 post is carried out column chromatography, then with the ethanol water eluting that concentration is 5%,
First order eluent；
D, the above-mentioned first order eluent Silon of 300 mesh is chromatographed as adsorbent, then use 1% aqueous acetic acid
Eluting, obtains second level eluent；
E, above-mentioned second level eluent ethanol is carried out recrystallization, after drying, obtain described mangosteen skin extract；
(2) by above-mentioned mangosteen skin extract 20 grams, gingerol 3 grams, 0.01 gram of sodium chloride, vitamin A 0.1 gram, vitamin E 0.1 gram,
Vitamin C 3 grams, Semen phaseoli radiati powder 0.1 gram, 0.01 gram of mixing and stirring of Thallus Porphyrae powder, the most i.e. obtain described mangosteen skin gingerol antioxygen
Change additive.
Embodiment 2
(1) mangosteen skin extract is prepared:
A, 4.5kg15% ethanol water will be added after 1kg mangosteen skin drying and crushing, extract 50 minutes in 55 DEG C of hot refluxs, and mistake
Filter concentrates, and obtains lixiviating solution；
B, above-mentioned lixiviating solution petroleum ether is extracted, obtain extract；
C, above-mentioned extract PEG 20000 post is carried out column chromatography, then with the ethanol water eluting that concentration is 8%,
First order eluent；
D, the above-mentioned first order eluent Silon of 300 mesh is chromatographed as adsorbent, then use 2% aqueous acetic acid
Eluting, obtains second level eluent；
E, above-mentioned second level eluent ethanol is carried out recrystallization, after drying, obtain described mangosteen skin extract；
(2) by above-mentioned mangosteen skin extract 25 grams, gingerol 4 grams, 0.015 gram of sodium chloride, vitamin A 0.15 gram, vitamin E 0.3
Gram, vitamin C 5 grams, Semen phaseoli radiati powder 0.15 gram, 0.015 gram of mixing and stirring of Thallus Porphyrae powder, the most i.e. obtain described mangosteen skin gingerol
Antioxidant.
Embodiment 3
(1) mangosteen skin extract is prepared:
A, 5kg20% ethanol water will be added after 1kg mangosteen skin drying and crushing, and extract 60 minutes in 60 DEG C of hot refluxs, and filter
Concentrate, obtain lixiviating solution；
B, above-mentioned lixiviating solution petroleum ether is extracted, obtain extract；
C, above-mentioned extract PEG 20000 post is carried out column chromatography, then with the ethanol water eluting that concentration is 10%,
Obtain first order eluent；
D, the above-mentioned first order eluent Silon of 300 mesh is chromatographed as adsorbent, then use 3% aqueous acetic acid
Eluting, obtains second level eluent；
E, above-mentioned second level eluent ethanol is carried out recrystallization, after drying, obtain described mangosteen skin extract；
(2) by above-mentioned mangosteen skin extract 30 grams, gingerol 5 grams, 0.02 gram of sodium chloride, vitamin A 0.2 gram, vitamin E 0.5 gram,
Vitamin C 8 grams, Semen phaseoli radiati powder 0.2 gram, 0.02 gram of mixing and stirring of Thallus Porphyrae powder, the most i.e. obtain described mangosteen skin gingerol antioxygen
Change additive.
The above, only presently preferred embodiments of the present invention, therefore the scope that the present invention implements can not be limited according to this, i.e. according to this
The equivalence change that patent of invention scope and description are made with modify, all should still belong in the range of the present invention contains.
Claims (4)
1. a mangosteen skin gingerol antioxidant, it is characterised in that: include the component of following weight portion:
Mangosteen skin extract 20-30 weight portion
Gingerol 3-5 weight portion
Sodium chloride 0.01-0.02 weight portion
Vitamin A 0.1-0.2 weight portion
Vitamin E 0.1-0.5 weight portion
Vitamin C 3-8 weight portion
Semen phaseoli radiati powder 0.1-0.2 weight portion
Thallus Porphyrae powder 0.01-0.02 weight portion
The extracting method of above-mentioned mangosteen skin extract is as follows:
A, extract adding ethanol water after above-mentioned mangosteen skin drying and crushing 40-60 minute in 50-60 DEG C of hot reflux, and mistake
Filter, obtains lixiviating solution；
B, above-mentioned lixiviating solution petroleum ether is extracted, obtain extract；
C, above-mentioned extract PEG 20000 post is carried out column chromatography, then be the ethanol aqueous wash of 5-10% by concentration
De-, obtain first order eluent；
D, above-mentioned first order eluent Silon is chromatographed as adsorbent, then use aqueous acetic acid eluting, obtain
Two grades of eluents；
E, above-mentioned second level eluent ethanol is carried out recrystallization, after drying, obtain described mangosteen skin extract.
2. the preparation method of the mangosteen skin gingerol antioxidant described in a claim 1, it is characterised in that: include as follows
Step:
(1) by mangosteen skin successively through ethanol water hot reflux extraction, petroleum ether extraction, PEG 20000 column chromatography, polyamides
The analysis of amine bisque, eluant ethanol recrystallization and dried, prepare described mangosteen skin extract, and this step comprises the steps:
A, extract adding ethanol water after above-mentioned mangosteen skin drying and crushing 40-60 minute in 50-60 DEG C of hot reflux, and mistake
Filter, obtains lixiviating solution；
B, above-mentioned lixiviating solution petroleum ether is extracted, obtain extract；
C, above-mentioned extract PEG 20000 post is carried out column chromatography, then be the ethanol aqueous wash of 5-10% by concentration
De-, obtain first order eluent；
D, above-mentioned first order eluent Silon is chromatographed as adsorbent, then use aqueous acetic acid eluting, obtain
Two grades of eluents；
E, above-mentioned second level eluent ethanol is carried out recrystallization, after drying, obtain described mangosteen skin extract；
(2) by above-mentioned mangosteen skin extract 20-30 weight portion, gingerol 3-5 weight portion, sodium chloride 0.01-0.02 weight portion, dimension life
Element A0.1-0.2 weight portion, vitamin E 0.1-0.5 weight portion and vitamin C 3-8 weight portion, Semen phaseoli radiati powder 0.1-0.2 weight portion,
Thallus Porphyrae powder 0.01-0.02 weight portion mixing and stirring, the most i.e. obtains described mangosteen skin gingerol antioxidant.
The preparation method of a kind of mangosteen skin gingerol antioxidant the most as claimed in claim 2, it is characterised in that: described step
Suddenly in step a of (1), the concentration of ethanol water is 10-20%, and this ethanol water gross weight is the Garcinia mangostana after drying and crushing
4-5 times of tare weight amount.
The preparation method of a kind of mangosteen skin gingerol antioxidant the most as claimed in claim 2, it is characterised in that: described step
Suddenly in step d of (1), described Silon is 300 mesh, and the concentration of described aqueous acetic acid is 1-3%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610372045.0A CN106107957A (en) | 2016-05-31 | 2016-05-31 | A kind of mangosteen skin gingerol antioxidant and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610372045.0A CN106107957A (en) | 2016-05-31 | 2016-05-31 | A kind of mangosteen skin gingerol antioxidant and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106107957A true CN106107957A (en) | 2016-11-16 |
Family
ID=57270641
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610372045.0A Pending CN106107957A (en) | 2016-05-31 | 2016-05-31 | A kind of mangosteen skin gingerol antioxidant and preparation method thereof |
Country Status (1)
| Country | Link |
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| CN (1) | CN106107957A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106912950A (en) * | 2017-03-27 | 2017-07-04 | 湖北文理学院 | A kind of durian peel cyclodextrin antioxidant and preparation method thereof |
| CN106954840A (en) * | 2017-03-27 | 2017-07-18 | 湖北文理学院 | A kind of gingerol compound stabilizer containing Rhizoma Gastrodiae extract |
| CN108283239A (en) * | 2017-05-25 | 2018-07-17 | 湖北文理学院 | A kind of soft sweets of the extract of slag containing pawpaw |
| CN111869872A (en) * | 2019-12-04 | 2020-11-03 | 湖北文理学院 | Laver antioxidant additive and preparation method thereof |
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| CN103211850A (en) * | 2013-04-23 | 2013-07-24 | 吉林化工学院 | Method for extracting flavone from mangosteen skin |
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| CN103211850A (en) * | 2013-04-23 | 2013-07-24 | 吉林化工学院 | Method for extracting flavone from mangosteen skin |
| CN105558927A (en) * | 2015-12-30 | 2016-05-11 | 湖北文理学院 | Apple seed oxidation preventing additive and preparation method thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106912950A (en) * | 2017-03-27 | 2017-07-04 | 湖北文理学院 | A kind of durian peel cyclodextrin antioxidant and preparation method thereof |
| CN106954840A (en) * | 2017-03-27 | 2017-07-18 | 湖北文理学院 | A kind of gingerol compound stabilizer containing Rhizoma Gastrodiae extract |
| CN108283239A (en) * | 2017-05-25 | 2018-07-17 | 湖北文理学院 | A kind of soft sweets of the extract of slag containing pawpaw |
| CN111869872A (en) * | 2019-12-04 | 2020-11-03 | 湖北文理学院 | Laver antioxidant additive and preparation method thereof |
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