CN106107635A - Utilize the method that Concha Ostreae fresh meat prepares Concha Ostreae oligopeptide - Google Patents
Utilize the method that Concha Ostreae fresh meat prepares Concha Ostreae oligopeptide Download PDFInfo
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- CN106107635A CN106107635A CN201610488510.7A CN201610488510A CN106107635A CN 106107635 A CN106107635 A CN 106107635A CN 201610488510 A CN201610488510 A CN 201610488510A CN 106107635 A CN106107635 A CN 106107635A
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Abstract
The invention discloses a kind of method utilizing Concha Ostreae fresh meat to prepare Concha Ostreae oligopeptide, concrete steps take the pulverizing of fresh Carnis ostreae, slurrying, obtain Concha Ostreae serosity；Add water in Concha Ostreae serosity, be warming up to 40～55 DEG C, regulate PH to 2.0～5.0；Add compound protease enzymolysis, be warming up to 80～95 DEG C of enzyme denaturing；Centrifugation, collects liquid phase；Liquid phase is used nanofiltration membrane separation, collects permeate；Will transmit through liquid and use electrodialysis desalination；Add beta-cyclodextrin and activated carbon refines, filter decarburization through filter press, collect liquid phase, be refined liquid；Refined liquid is concentrated by membrance concentration equipment, is spray-dried, obtains the oligomeric Gly-His-Lys of Concha Ostreae.The method that the present invention utilizes Concha Ostreae fresh meat to prepare Concha Ostreae oligopeptide improves Concha Ostreae added value, in products obtained therefrom, Concha Ostreae oligopeptide content is high, molecular weight is at below 1000Da, it is possible to fully absorbed by human body, has extremely important realistic meaning to promoting China's marine industries to develop rapidly.
Description
Technical field
The present invention relates to Concha Ostreae oligopeptide preparation field.More particularly, to one with Concha Ostreae fresh meat as raw material, utilize multiple
The method that hop protein enzyme enzymolysis prepares Concha Ostreae oligopeptide.
Background technology
Concha Ostreae is the first big cultivated shellfish, one of big culturing economic shellfish of Ye Shi China famous 4 in the world.Concha Ostreae software contains
There are the nutritional labelings such as rich in protein, glycogen, taurine, aminoacid, there is huge edible and medical value.Peptide is albumen
Matter hydrolysis intermediate product, generally, by by 2～10 Amino acid profiles be referred to as oligopeptide, will be by 11～50 aminoacid groups
Become is referred to as polypeptide.On material is constituted, peptide and protein have homogeneity, but have the uniqueness being different from amino acid and protein
Physiological function, especially oligopeptide.Scientific investigations showed that, the principal mode of absorption of human body protein is to inhale with the form of peptide
Receive.Oligopeptide absolutely can be absorbed by the body with complete form.
The technique being obtained oyster peptide in prior art with Concha Ostreae for raw material by enzymolysis, gained peptide molecular weight is big, be difficult to by
Absorption of human body, and with bitterness, fishy smell, mouthfeel extreme difference.
Summary of the invention
It is an object of the invention to exploitation a kind of with Concha Ostreae fresh meat as raw material, preparation can be fully absorbed by human body, without bitter
Taste, the method for oligopeptide of fishy smell so that prepared Concha Ostreae oligopeptide directly can eat as nutrient protein supplement.
For reaching above-mentioned purpose, the present invention provides a kind of method utilizing Concha Ostreae fresh meat to prepare Concha Ostreae oligopeptide, specifically walks
Rapid as follows:
S1, pretreatment of raw material: take the pulverizing of fresh Carnis ostreae, slurrying, obtain Concha Ostreae serosity, use kjeldahl determination to record described
Total protein content in Concha Ostreae serosity；
S2, to step S1 prepare Concha Ostreae serosity in add described Concha Ostreae serosity 2～the water of 5 times of quality, be warming up to 40～
After 55 DEG C, add the hydrochloric acid solution regulation PH to 2.0～5.0 of 2.5mol/L；Add account for described Concha Ostreae serous protein quality 1.0～
The compound protease of 5.0%, after stirring enzymolysis 3～6h, is warming up to 80～95 DEG C of enzyme denaturing 10～25min, obtains enzymolysis solution；By institute
State enzymolysis solution centrifugation, collect liquid phase；
The quality proportioning of described compound protease is: pepsin: aspergillus niger As3.350 protease: the U.S. inulinase 537 of space assistant
Protease=2～4:3～5:3～5；
S3, liquid phase step S2 obtained use the nanofiltration membrane separation of 2000～8000Da, collect permeate；
S4, permeate step S3 collected use electrodialysis desalination；Add beta-cyclodextrin and activated carbon, add
The hydrochloric acid of 2.5mol/L corrects described permeate PH to 5～7, is warming up to 50～80 DEG C, after reaction 30～45min carries out refining,
Filter decarburization through filter press, collect liquid phase, be refined liquid；
The addition of described beta-cyclodextrin is the 0.01～0.05% of described permeate quality, the addition of described activated carbon
Amount is the 0.1～1.0% of described permeate quality；
S5, refined liquid step S4 prepared are concentrated into soluble solid content by membrance concentration equipment, and to account for concentrated solution total
Quality 20～40%, is spray-dried, obtains the oligomeric Gly-His-Lys of Concha Ostreae.
Under optimal way, step S1 particularly as follows: take fresh Carnis ostreae, utilize meat grinder by described Carnis ostreae rub 10～
20min, obtains Concha Ostreae serosity.
Under optimal way, the condition of centrifugation described in step S2 is: using rocking type channel separator, rotating speed is
10000～16000r/min.
Under optimal way, described in step S4, electrodialytic operating condition is: voltage 10～100V, enters to separate flow velocity 1.0
～1.55 ton hour, concentrated stream speed 1.0～1.60 ton hour, cooling of electrode water flow velocity 0.5～1.0 ton hour.
Under optimal way, the operating condition of membrance concentration described in step S5 is: the nanofiltration using separating ranges to be 10～100Da
Level, hollow-fibre membrane, operation pressure is 1.5～1.8Mpa.
Under optimal way, the condition being spray-dried described in step S5 is: inlet temperature 140 DEG C, leaving air temp 85 DEG C.
The present invention having the beneficial effects that compared to prior art:
1, the inventive method prepare Concha Ostreae oligomeric Gly-His-Lys more than molecular weight 2000Da account for 0.03%～0.1%,
2000Da-1000Da accounts for 0.3%～0.4%, and 1000Da-500Da accounts for 8%～9%, 500Da-180Da account for 57%～
58%, below 180Da account for 33%～34%.The molecular weight product that the inventive method the prepares Concha Ostreae oligopeptide less than 1000Da
Content is higher, it is possible to fully absorbed by human body.
2, the oligomeric Gly-His-Lys of Concha Ostreae that the inventive method prepares is without bitterness, fishy smell, directly can eat as nutrient protein supplement
With.
3, the present invention direct enzymolysis of Concha Ostreae fresh meat, effectively make use of the autolytic enzyme of Concha Ostreae fresh meat self, reduces multiple
The addition of hop protein enzyme, has saved product cost.
To sum up, the method that the present invention utilizes Concha Ostreae fresh meat to prepare Concha Ostreae oligopeptide improves Concha Ostreae added value, products obtained therefrom
Middle Concha Ostreae oligopeptide content is high, and molecular weight is at below 1000Da, it is possible to fully absorbed by human body, to promoting China's marine industries
Develop rapidly and there is extremely important realistic meaning.
Detailed description of the invention
Following instance is to further illustrate the present invention.
Embodiment 1
(1) Concha Ostreae fresh meat 1000kg meat grinder is pulverized 15min, enter colloid mill defibrination after rubbing and obtain serosity, utilize
Kjeldahl determination records protein content in Concha Ostreae serosity, determines albumen gross mass in Concha Ostreae serosity, and then determines enzyme concentration.
(2) serosity is imported 5m3Enzymatic vessel, described 5m3Enzymatic vessel is sandwich-type reacting by heating still, adds 3 times of Concha Ostreae serosity
The pure water of quality, adjusts system temperature 50 DEG C, adjusts PH to 2.0 with the HCL solution of 2.5mol/L, adds the described total egg of Concha Ostreae serosity
The compound protease of white matter amount 2.5%；The quality proportioning of compound protease is: pepsin: aspergillus niger As3.350 protease:
The U.S. inulinase 537 protease=3:4:4 of space assistant, stirs enzymolysis 4h, and heat up 80 DEG C of enzyme denaturing 20min, uses tube centrifuge
16000rpm is centrifugal carries out solid-liquid separation, collects centrifugal clear liquid, imports 5m3Clear liquid storage tank.
(3)5m3Separate liquid storage tank to be connected with membrane separation device, gained clear liquid is separated through 5000Da nanofiltration membrane separation device
Obtain permeate；
(4) membrane separation device is connected with electrodialysis plant, by gained permeate after inorganic ions is removed in electrodialysis, imports
5m3Treatment tank is refined through row.Electrodialytic voltage is 75V, enters to separate flow velocity 1.5 ton hour, concentrated stream speed 1.52 tons/little
Time, cooling of electrode water flow velocity 0.8 ton hour.
(5) described 5m3Treatment tank is sandwich-type reacting by heating still, and refined condition is: add the β of permeate quality 0.02%
Cyclodextrin, add 0.8% activated carbon of permeate quality, correct with the HCL of 2.5mol/L and separate liquid PH to 6, be warmed to 65 DEG C,
Reaction 40min.
(6) described 5m3Treatment tank is connected with filter press, through filter press decarburization, obtains refined liquid.
(8) described filter press is connected with membrance concentration equipment, refined liquid is concentrated through 50Da NF membrane, uses
1.6mpa pressure, is concentrated into solubility solid content and accounts for concentrated solution quality 30%, import and concentrate storage tank.
(9) described concentration storage tank, is provided with discharging opening, concentration feed liquid imports after discharging opening takes out spray drying tower and enters
Row is spray-dried, and during spray drying, inlet temperature maintains 140 DEG C, and leaving air temp maintains 85 DEG C, obtains the oligomeric Gly-His-Lys of Concha Ostreae.
The oligomeric Gly-His-Lys of Concha Ostreae 59.1 kilograms that the present embodiment prepares, color is milky, and non-metallic ion remains, and kelvin is fixed
It is 8.21% that the method for nitrogen surveys protein content in Concha Ostreae serosity, according to Tot Prot calculated yield in raw material then 59.1/
(8.21%*1000)=0.72=72%；Yield 72%.Wherein kjeldahl determination survey Concha Ostreae oligomeric Gly-His-Lys total nitrogen content can reach
12.4%, what high performance liquid chromatography recorded Concha Ostreae oligopeptide more than molecular weight 2000Da accounts for 0.05%, 2000Da-1000Da's
Accounting for 0.37%, 1000Da-500Da accounts for 8.21%, and 500Da-180Da accounts for 57.44%, and below 180Da accounts for 33.93%.
DNS method surveys contents of monosaccharides 2.8%, without fishy smell, without bitterness, is the Concha Ostreae oligopeptide of high-quality.
The above, the only present invention preferably detailed description of the invention, but protection scope of the present invention is not limited thereto,
Any those familiar with the art in the technical scope of present disclosure, according to technical scheme and
Inventive concept equivalent or change in addition, all should contain within protection scope of the present invention.
Claims (7)
1. one kind utilizes the method that Concha Ostreae fresh meat prepares Concha Ostreae oligopeptide, it is characterised in that specifically comprise the following steps that
S1, pretreatment of raw material: take the pulverizing of fresh Carnis ostreae, slurrying, obtain Concha Ostreae serosity, use kjeldahl determination to record described Concha Ostreae
Total protein content in serosity；
S2, to step S1 prepare Concha Ostreae serosity in add described Concha Ostreae serosity 2～the water of 5 times of quality, be warming up to 40～55 DEG C
After, add the hydrochloric acid solution regulation PH to 2.0～5.0 of 2.5mol/L；Add account for described Concha Ostreae serous protein quality 1.0～
The compound protease of 5.0%, after stirring enzymolysis 3～6h, is warming up to 80～95 DEG C of enzyme denaturing 10～25min, obtains enzymolysis solution；By institute
State enzymolysis solution centrifugation, collect liquid phase；
The quality proportioning of described compound protease is: pepsin: aspergillus niger As3.350 protease: U.S. inulinase 537 albumen of space assistant
Enzyme=2～4:3～5:3～5；
S3, liquid phase step S2 obtained use the nanofiltration membrane separation of 2000～8000Da, collect permeate；
S4, permeate step S3 collected use electrodialysis desalination；Add beta-cyclodextrin and activated carbon, add 2.5mol/L
Hydrochloric acid correct described permeate PH to 5～7, be warming up to 50～80 DEG C, reaction 30～45min carry out refined after, through sheet frame pressure
Filter filters decarburization, collects liquid phase, is refined liquid；
The addition of described beta-cyclodextrin is the 0.01～0.05% of described permeate quality, and the addition of described activated carbon is
The 0.1～1.0% of described permeate quality；
S5, the refined liquid that step S4 prepares by membrance concentration equipment, is concentrated into soluble solid content and accounts for concentrated solution gross mass
20～40%, it is spray-dried, obtains the oligomeric Gly-His-Lys of Concha Ostreae.
Utilize the method that Concha Ostreae fresh meat prepares Concha Ostreae oligopeptide the most according to claim 1, it is characterised in that step S1 is concrete
For: take fresh Carnis ostreae, utilize meat grinder that described Carnis ostreae is rubbed 10～20min, obtain Concha Ostreae serosity.
Utilize the method that Concha Ostreae fresh meat prepares Concha Ostreae oligopeptide the most according to claim 1, it is characterised in that step S2 adds
The hydrochloric acid solution regulation PH to 3.0 of 2.5mol/L.
Utilize the method that Concha Ostreae fresh meat prepares Concha Ostreae oligopeptide the most according to claim 1, it is characterised in that described in step S2
The condition of centrifugation is: using rocking type channel separator, rotating speed is 10000～16000r/min.
Utilize the method that Concha Ostreae fresh meat prepares Concha Ostreae oligopeptide the most according to claim 1, it is characterised in that described in step S4
Electrodialytic operating condition is: voltage 10～100V, enters to separate flow velocity 1.0～1.55 ton hour, concentrated stream speed 1.0～
1.60 ton hour, cooling of electrode water flow velocity 0.5～1.0 ton hour.
Utilize the method that Concha Ostreae fresh meat prepares Concha Ostreae oligopeptide the most according to claim 1, it is characterised in that described in step S5
The operating condition of membrance concentration is: use separating ranges be 10～100Da nanofiltration level, hollow-fibre membrane, operation pressure be 1.5～
1.8Mpa。
Utilize the method that Concha Ostreae fresh meat prepares Concha Ostreae oligopeptide the most according to claim 1, it is characterised in that described in step S5
The condition being spray-dried is: inlet temperature 140 DEG C, leaving air temp 85 DEG C.
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| CN107319400A (en) * | 2017-06-15 | 2017-11-07 | 大连豪翔生物酶工程有限公司 | Bioactive ingredients method for integrated extraction in oyster |
| CN107691760A (en) * | 2017-09-21 | 2018-02-16 | 青岛海洋生物医药研究院股份有限公司 | A kind of oyster peptide of enhancing development and its preparation method and application |
| CN107893095A (en) * | 2017-12-19 | 2018-04-10 | 大连深蓝肽科技研发有限公司 | A kind of method that marine source albumen prepares high F value oligopeptide |
| CN107997184A (en) * | 2017-12-13 | 2018-05-08 | 国药肽谷有限公司 | The preparation method of one seed oyster oligopeptide product |
| CN108060151A (en) * | 2017-12-19 | 2018-05-22 | 大连深蓝肽科技研发有限公司 | Oyster oligopeptide prepares specific complex protease preparation and its application method in high yield |
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| CN111659648A (en) * | 2020-05-24 | 2020-09-15 | 海南华肽生物科技有限公司 | Oyster peptide processing device and processing method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107319400A (en) * | 2017-06-15 | 2017-11-07 | 大连豪翔生物酶工程有限公司 | Bioactive ingredients method for integrated extraction in oyster |
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| CN107997184A (en) * | 2017-12-13 | 2018-05-08 | 国药肽谷有限公司 | The preparation method of one seed oyster oligopeptide product |
| CN107893095A (en) * | 2017-12-19 | 2018-04-10 | 大连深蓝肽科技研发有限公司 | A kind of method that marine source albumen prepares high F value oligopeptide |
| CN108060151A (en) * | 2017-12-19 | 2018-05-22 | 大连深蓝肽科技研发有限公司 | Oyster oligopeptide prepares specific complex protease preparation and its application method in high yield |
| CN108129552A (en) * | 2017-12-22 | 2018-06-08 | 大连深蓝肽科技研发有限公司 | The antioxidant activity peptide fragment and extracting method in a kind of sea cucumber source |
| CN108085355A (en) * | 2017-12-22 | 2018-05-29 | 大连深蓝肽科技研发有限公司 | A kind of preparation method of microencapsulation oyster oligopeptide |
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| WO2020224058A1 (en) * | 2019-05-09 | 2020-11-12 | 烟台嘉惠海洋生物科技有限公司 | Industrialized production method for preparing oyster peptide by means of enzymatic method |
| CN110959562A (en) * | 2019-12-25 | 2020-04-07 | 上海海洋大学 | Application of oyster peptide in promoting adhesion of Shewanella maritima induced Mytilus coruscus larvae |
| CN111659648A (en) * | 2020-05-24 | 2020-09-15 | 海南华肽生物科技有限公司 | Oyster peptide processing device and processing method |
| CN111903970A (en) * | 2020-08-13 | 2020-11-10 | 安徽盛美诺生物技术有限公司 | Method for preparing Concha Ostreae extract for improving male sexual function and relieving fatigue |
| CN114395594A (en) * | 2022-01-30 | 2022-04-26 | 漳州卫生职业学院 | Preparation method and application of oyster small molecular peptide |
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